Technical Notes

System Architecture

Core Components

  • Dual Stress Sensors: σ32 (cytosolic) and σE/Cpx (envelope)
  • ProductionMode Gate: (Scyt ∨ Senv) ∧ PM logic
  • Actuator & Latch: Flp(ssrA) recombinase with FRT cassette
  • Translation Certificate: Ribosomal re-initiation mechanism
  • Antidote: Dual transcriptional and sRNA inhibition

Partitioning Strategy

The system is divided between genome-integrated components (sensors, gate, actuator, latch) and plasmid-based elements (target protein, certificate, ProductionMode).

Implementation Details

Stress Pathway Integration

The system leverages native E. coli stress response pathways without requiring genetic modifications to the host chassis.

Quality Control Metrics

  • Target enrichment: ≥10×
  • False commit risk: ≤0.001
  • ProductionMode threshold: tunable parameter

References

  • σ32 heat shock response pathway
  • σE/Cpx envelope stress sensing
  • Flp/FRT recombination system
  • Ribosomal re-initiation mechanisms