import gradio as gr
import joblib
from huggingface_hub import hf_hub_download
import pandas as pd
import numpy as np
from collections import Counter
import os
os.environ["GRADIO_SSR_MODE"] = "false"
os.environ["GRADIO_ANALYTICS_ENABLED"] = "false"

import time
print("STARTING APP...")
time.sleep(2)
print("APP READY")

# ➕ ADDED: placeholders (instead of loading at startup)
model_dict = None
feature_columns = None
model_package = None

# ❌ REMOVED: direct model loading at startup
# repo_id = "Ym420/Peptide-Function"
# model_filename = "xgb_multilabel_model_full.pkl"
# model_path = hf_hub_download(repo_id=repo_id, filename=model_filename)
# model_package = joblib.load(model_path)
# model_dict = model_package['model']
# feature_columns = model_package['feature_columns']

# ➕ ADDED: lazy loader function
def init_model():
    global model_dict, feature_columns, model_package
    
    if model_dict is None:
        repo_id = "Ym420/Peptide-Function"
        model_filename = "xgb_multilabel_model_full.pkl"

        model_path = hf_hub_download(repo_id=repo_id, filename=model_filename)
        model_package = joblib.load(model_path)

        model_dict = model_package['model']
        feature_columns = model_package['feature_columns']

        # 🔁 MOVED: metadata loading (was global before)
        global aa_list, dipeptides, hydrophobicity_scale, eisenberg_scale
        global aa_mass, aa_charge, aa_boman, aa_flexibility
        global aa_polarizability, aa_aliphatic, aa_deltaG, aa_pucker

        aa_list = model_package.get('aa_list', [])
        dipeptides = model_package.get('dipeptides', [])
        hydrophobicity_scale = model_package.get('hydrophobicity_scale', {})
        eisenberg_scale = model_package.get('eisenberg_scale', {})
        aa_mass = model_package.get('aa_mass', {})
        aa_charge = model_package.get('aa_charge', {})
        aa_boman = model_package.get('aa_boman', {})
        aa_flexibility = model_package.get('aa_flexibility', {})
        aa_polarizability = model_package.get('aa_polarizability', {})
        aa_aliphatic = model_package.get('aa_aliphatic', {})
        aa_deltaG = model_package.get('aa_deltaG', {})
        aa_pucker = model_package.get('aa_pucker', {})

# --- Target cells ---
TARGET_CELLS = ["Gram+", "Fungus", "Mammalian Cell", "Cancer", "Gram-"]

# --- Feature extraction ---
def extract_features_app(seq: str) -> pd.DataFrame:
    seq = seq.upper()
    
    count = Counter([seq[i:i+2] for i in range(len(seq)-1)])
    total = max(len(seq)-1, 1)
    dipep_features = [count.get(dp, 0) / total for dp in dipeptides]

    def g(aa, table): return table.get(aa, 0)
    def h(dp, table): return (g(dp[0], table) + g(dp[1], table)) / 2.0

    dipeptides_seq = [seq[i:i+2] for i in range(len(seq)-1)]
    
    if len(seq) < 2:
        physchem_features = [0]*13
    else:
        mw = np.mean([h(dp, aa_mass) for dp in dipeptides_seq])
        charge = np.mean([h(dp, aa_charge) for dp in dipeptides_seq])
        hydro = np.mean([h(dp, hydrophobicity_scale) for dp in dipeptides_seq])
        aromatic = np.mean([(dp[0] in 'FWY') + (dp[1] in 'FWY') for dp in dipeptides_seq]) / 2.0
        pI = np.mean([h(dp, {aa: 7 + (int(aa in 'KRH') - int(aa in 'DE')) for aa in aa_list}) for dp in dipeptides_seq])
        instability = np.mean([((dp[0] in 'DEKR') + (dp[1] in 'DEKR')) / 2.0 for dp in dipeptides_seq])
        hydro_moment = np.sqrt(np.mean([(h(dp, eisenberg_scale))**2 for dp in dipeptides_seq]))
        aliphatic = np.mean([h(dp, aa_aliphatic) for dp in dipeptides_seq])
        boman = np.mean([h(dp, aa_boman) for dp in dipeptides_seq])
        flexibility = np.mean([h(dp, aa_flexibility) for dp in dipeptides_seq])
        polarizability = np.mean([h(dp, aa_polarizability) for dp in dipeptides_seq])
        deltag = np.mean([h(dp, aa_deltaG) for dp in dipeptides_seq])
        pucker = np.mean([h(dp, aa_pucker) for dp in dipeptides_seq])

        physchem_features = [mw, charge, hydro, aromatic, pI, instability,
                             hydro_moment, aliphatic, boman, flexibility, polarizability, deltag, pucker]
    
    features = dipep_features + physchem_features
    
    df = pd.DataFrame([features], columns=feature_columns)
    df = df.astype('float32')
    return df

# --- Prediction function ---
def predict_peptide(sequence: str):
    init_model()   # ➕ ADDED: ensures model loads only when needed

    seq = "".join(sequence.split()).upper()
    if not seq:
        return []

    X = extract_features_app(seq)
    
    table = []
    for target in TARGET_CELLS:
        clf = model_dict.get(target)
        if clf is not None:
            prob = clf.predict_proba(X)[0][1]
            table.append([target, round(float(prob), 4)])
        else:
            table.append([target, None])

    return table

# --- Gradio Interface ---
custom_css = """
footer, .footer {display:none !important;}
"""

#with gr.Blocks(css=custom_css, theme="default") as demo:
with gr.Blocks(css=custom_css) as demo:
    gr.Markdown("## AMP Spectrum")
    
    seq_input = gr.Textbox(label="Enter Peptide Sequence")
    
    with gr.Row():
        predict_btn = gr.Button("Predict", variant="primary")
        clear_btn = gr.Button("Clear")
    
    table_output = gr.Dataframe(
        headers=["Target", "Confidence"],
        datatype=["str","number"],
        interactive=False
    )
    
    predict_btn.click(fn=predict_peptide, inputs=seq_input, outputs=table_output)
    clear_btn.click(fn=lambda: ("", []), outputs=[seq_input, table_output])
    
    gr.api(predict_peptide, api_name="predict_peptide")

if __name__ == "__main__": 
    demo.launch(
    server_name="0.0.0.0",
    server_port=7860,
    root_path="/",
    show_error=True,
    ssr_mode=False
)