""" Improved larva counting application for Hugging Face Spaces. This version exposes additional parameters through Gradio sliders to allow the user to tune the preprocessing and contour‑filtering steps. It also modularises some of the hard‑coded constants in the original implementation. Parameters exposed via the UI: * **Umbral**: Threshold value for binary segmentation. Setting 0 triggers Otsu's automatic threshold. * **Área mínima / máxima**: Rejects contours outside this size range. * **Forma mínima / máxima**: Controls the acceptable ellipse axis ratio of detected contours; values between 0 and 1. Useful for eliminating elongated or highly circular artefacts. * **Solidez mínima**: Rejects contours with low solidity (area divided by convex hull area), which helps to discard irregular flour particles. * **Kernel morfológico**: Size of the structuring element used during the morphological opening step; larger kernels remove more noise but can merge nearby larvae. * **Iteraciones morfológicas**: Number of times the morphological opening is applied. * **CLAHE clipLimit** and **tileGridSize**: Adjust the contrast limited adaptive histogram equalisation used to emphasise bright dots in the image. * **Gauss blur (fondo)**: Kernel size for the Gaussian blur that estimates the background. Larger kernels remove broader illumination gradients. * **Median blur**: Kernel size for the median filter used to smooth the preprocessed image. * **Border recorte**: Number of pixels trimmed from each edge of the resized image. Adjust if the frame contains noise or if larvae are close to the border. The counting logic remains similar to the original: after thresholding and morphological filtering, contours are filtered by area, shape and solidity. For contours larger than the maximum single‑larva area, the estimated number of larvae is computed by dividing by the median area of small contours. """ import gradio as gr import cv2 import numpy as np import statistics # ----- Constants ----- # Target image size; images are resized to this resolution for processing. IMG_W = 2047 IMG_H = 1148 # Default parameters used when sliders are at their initial positions. DEFAULT_BORDER = 6 DEFAULT_CLIP = 2.5 DEFAULT_TILE = 8 DEFAULT_BG_BLUR = 25 DEFAULT_MEDIAN_BLUR = 3 DEFAULT_SHAPE_MIN = 0.55 DEFAULT_SHAPE_MAX = 0.95 DEFAULT_MIN_SOLIDITY = 0.7 # Global state for accumulated count. In a production system you could use # gr.State or another mechanism to avoid globals. global_count = 0 median_single_area = None def ellipse_ratio(cnt: np.ndarray) -> float | None: """Return the minor/major axis ratio of the best‑fit ellipse for a contour. If the contour has fewer than 5 points (required by cv2.fitEllipse), returns ``None``. Args: cnt: Contour as returned by ``cv2.findContours``. Returns: float between 0 and 1, or ``None`` if fitting fails. """ if len(cnt) < 5: return None try: (_, _), (MA, ma), _ = cv2.fitEllipse(cnt) except cv2.error: return None # ratio of minor axis to major axis return min(MA, ma) / max(MA, ma) def contour_solidity(cnt: np.ndarray) -> float: """Compute the solidity of a contour (area divided by convex hull area).""" area = cv2.contourArea(cnt) if area <= 0: return 0.0 hull = cv2.convexHull(cnt) hull_area = cv2.contourArea(hull) if hull_area == 0: return 0.0 return float(area) / float(hull_area) def preprocess( image_bgr: np.ndarray, clip_limit: float = DEFAULT_CLIP, tile_grid_size: int = DEFAULT_TILE, bg_blur: int = DEFAULT_BG_BLUR, median_blur: int = DEFAULT_MEDIAN_BLUR, border: int = DEFAULT_BORDER, ) -> tuple[np.ndarray, np.ndarray]: """Resize and enhance the input image. The function performs the following steps: 1. Resize the image to ``IMG_W`` × ``IMG_H`` using bilinear interpolation. 2. Crop ``border`` pixels from each side. 3. Convert to grayscale. 4. Apply CLAHE to emphasise bright points. 5. Subtract a blurred background to remove gradients. 6. Normalise to full 0‑255 range. 7. Apply median filtering to reduce noise. Args: image_bgr: Original image in BGR colour space. clip_limit: CLAHE clip limit; higher values increase local contrast. tile_grid_size: Size of the grid for CLAHE (in pixels). The same value is used for both dimensions. bg_blur: Kernel size (odd integer) for the Gaussian blur used to estimate the background. median_blur: Kernel size (odd integer) for the median filter. border: Number of pixels to trim from each edge after resizing. Returns: A tuple ``(sub, img)`` where ``sub`` is the processed grayscale image and ``img`` is the resized colour image (for overlaying detections). """ # Resize to a consistent working resolution img = cv2.resize(image_bgr, (IMG_W, IMG_H), interpolation=cv2.INTER_LINEAR) # Crop the border region if border > 0: roi = img[border : IMG_H - border, border : IMG_W - border] else: roi = img.copy() # Convert to grayscale gray = cv2.cvtColor(roi, cv2.COLOR_BGR2GRAY) # Apply CLAHE (Contrast Limited Adaptive Histogram Equalization) tile_size = max(1, int(tile_grid_size)) clahe = cv2.createCLAHE(clipLimit=float(clip_limit), tileGridSize=(tile_size, tile_size)) gray = clahe.apply(gray) # Estimate background via Gaussian blur # Ensure the blur kernel is odd and at least 3 bg_blur = int(bg_blur) if int(bg_blur) % 2 == 1 else int(bg_blur) + 1 bg = cv2.GaussianBlur(gray, (bg_blur, bg_blur), 0) # Subtract background and normalise sub = cv2.subtract(gray, bg) sub = cv2.normalize(sub, None, 0, 255, cv2.NORM_MINMAX) # Median blur to reduce noise from flour granules m_size = int(median_blur) if int(median_blur) % 2 == 1 else int(median_blur) + 1 sub = cv2.medianBlur(sub, m_size) return sub, img def detect_larvas( image_bgr: np.ndarray, thresh_value: int = 10, min_area: int = 6, max_area_single: int = 40, shape_min: float = DEFAULT_SHAPE_MIN, shape_max: float = DEFAULT_SHAPE_MAX, min_solidity: float = DEFAULT_MIN_SOLIDITY, morph_kernel: int = 3, morph_iter: int = 1, clip_limit: float = DEFAULT_CLIP, tile_grid_size: int = DEFAULT_TILE, bg_blur: int = DEFAULT_BG_BLUR, median_blur: int = DEFAULT_MEDIAN_BLUR, border: int = DEFAULT_BORDER, ) -> tuple[np.ndarray, int]: """Detect and count larvae in the input image. Applies preprocessing, thresholding, morphological filtering and contour analysis. Contours are filtered by area, ellipse axis ratio and solidity. Large contours are divided by the median area of single larvae to estimate the number of larvae they contain. Args: image_bgr: Original image in BGR colour space. thresh_value: Threshold for binarisation; 0 triggers Otsu's method. min_area: Minimum contour area to accept (in pixels²). max_area_single: Maximum area considered as one larva (in pixels²). shape_min, shape_max: Acceptable range of ellipse axis ratio. min_solidity: Minimum solidity to accept a contour. morph_kernel: Size of the morphological kernel (odd integer). morph_iter: Number of morphological opening iterations. clip_limit, tile_grid_size, bg_blur, median_blur, border: Parameters passed to ``preprocess``. Returns: A tuple ``(output_image, total)`` where ``output_image`` is the colour image with contours drawn and ``total`` is the estimated number of larvae. """ global median_single_area gray_proc, base_img = preprocess( image_bgr, clip_limit=clip_limit, tile_grid_size=tile_grid_size, bg_blur=bg_blur, median_blur=median_blur, border=border, ) # Thresholding if thresh_value == 0: # Otsu's threshold _, th = cv2.threshold(gray_proc, 0, 255, cv2.THRESH_BINARY + cv2.THRESH_OTSU) else: _, th = cv2.threshold(gray_proc, int(thresh_value), 255, cv2.THRESH_BINARY) # Morphological opening to remove small noise k_size = int(morph_kernel) # Ensure kernel size is odd and >= 1 if k_size < 1: k_size = 1 if k_size % 2 == 0: k_size += 1 kernel = np.ones((k_size, k_size), np.uint8) iters = max(1, int(morph_iter)) th = cv2.morphologyEx(th, cv2.MORPH_OPEN, kernel, iterations=iters) # Find external contours contours, _ = cv2.findContours(th, cv2.RETR_EXTERNAL, cv2.CHAIN_APPROX_SIMPLE) good = [] areas_all = [] areas_single = [] for c in contours: area = cv2.contourArea(c) # Discard extremely large regions (limit at 5000 px² as in original) if area < min_area or area > 5000: continue # Shape ratio filter ratio = ellipse_ratio(c) if ratio is None or not (shape_min <= ratio <= shape_max): continue # Solidity filter sol = contour_solidity(c) if sol < min_solidity: continue good.append(c) areas_all.append(area) if area <= max_area_single: areas_single.append(area) # Estimate median area of single larvae if areas_single: median_single_area = statistics.median_low(areas_single) elif areas_all: median_single_area = statistics.median_low(areas_all) else: # No detections out = base_img.copy() cv2.putText(out, "LARVAS: 0", (40, 80), cv2.FONT_HERSHEY_SIMPLEX, 2, (0, 0, 255), 3) return out, 0 # Count larvae total = 0 for c in good: a = cv2.contourArea(c) if a <= max_area_single: total += 1 else: # Estimate number of larvae in a cluster est = int(round(a / median_single_area)) total += max(1, est) # Draw contours on the original‑sized image out = base_img.copy() for c in good: # Shift contour coordinates by the border offset if border > 0: c_shifted = c + np.array([[border, border]]) else: c_shifted = c cv2.drawContours(out, [c_shifted], -1, (0, 255, 0), 1) cv2.putText(out, f"LARVAS: {total}", (40, 80), cv2.FONT_HERSHEY_SIMPLEX, 2, (0, 0, 255), 3) return out, total def process( image: np.ndarray, thresh: int, min_a: int, max_a: int, shape_min: float, shape_max: float, sol_min: float, morph_kernel: int, morph_iter: int, clip_limit: float, tile_grid: int, bg_blur: int, med_blur: int, border: int, ) -> tuple[np.ndarray | None, str, str]: """Gradio wrapper for larva detection. Accumulates the total count across multiple calls via the global ``global_count`` variable. """ global global_count if image is None: return None, "No subiste imagen", f"Conteo total: {global_count}" img_bgr = cv2.cvtColor(np.array(image), cv2.COLOR_RGB2BGR) out_img_bgr, n = detect_larvas( img_bgr, thresh_value=int(thresh), min_area=int(min_a), max_area_single=int(max_a), shape_min=float(shape_min), shape_max=float(shape_max), min_solidity=float(sol_min), morph_kernel=int(morph_kernel), morph_iter=int(morph_iter), clip_limit=float(clip_limit), tile_grid_size=int(tile_grid), bg_blur=int(bg_blur), median_blur=int(med_blur), border=int(border), ) global_count += n out_img_rgb = cv2.cvtColor(out_img_bgr, cv2.COLOR_BGR2RGB) return out_img_rgb, f"Larvas en la imagen: {n}", f"Conteo total: {global_count}" def reset_count() -> str: """Reset the accumulated count and return a message.""" global global_count global_count = 0 return f"Conteo total: {global_count}" # ----- Gradio interface ----- with gr.Blocks() as demo: gr.Markdown("## Contador de larvas – versión mejorada") with gr.Row(): # Input column with gr.Column(scale=1): inp = gr.Image(label="Subí la foto") thresh = gr.Slider(0, 255, value=10, step=1, label="Umbral (0=Otsu auto)") min_area = gr.Slider(0, 300, value=6, step=1, label="Min área px²") max_area_single = gr.Slider(0, 5000, value=40, step=1, label="Máx área 1 larva px²") shape_min_s = gr.Slider(0.0, 1.0, value=DEFAULT_SHAPE_MIN, step=0.05, label="Forma mínima") shape_max_s = gr.Slider(0.0, 1.0, value=DEFAULT_SHAPE_MAX, step=0.05, label="Forma máxima") solidity_min_s = gr.Slider(0.0, 1.0, value=DEFAULT_MIN_SOLIDITY, step=0.05, label="Solidez mínima") morph_kernel_s = gr.Slider(3, 11, value=3, step=2, label="Kernel morfológico") morph_iter_s = gr.Slider(1, 3, value=1, step=1, label="Iteraciones morfológicas") cliplimit_s = gr.Slider(1.0, 5.0, value=DEFAULT_CLIP, step=0.5, label="CLAHE clipLimit") tilegrid_s = gr.Slider(4, 16, value=DEFAULT_TILE, step=2, label="CLAHE tileGridSize") bg_blur_s = gr.Slider(15, 55, value=DEFAULT_BG_BLUR, step=2, label="Gauss blur (fondo)") median_blur_s = gr.Slider(3, 11, value=DEFAULT_MEDIAN_BLUR, step=2, label="Median blur") border_s = gr.Slider(0, 20, value=DEFAULT_BORDER, step=1, label="Border recorte") btn = gr.Button("Procesar") btn_reset = gr.Button("Reset contador") # Output column with gr.Column(scale=1): out_img = gr.Image(label="Resultado") out_txt = gr.Textbox(label="Resultado individual") out_total = gr.Textbox(label="Resultado acumulado") # Bind button clicks btn.click( process, inputs=[ inp, thresh, min_area, max_area_single, shape_min_s, shape_max_s, solidity_min_s, morph_kernel_s, morph_iter_s, cliplimit_s, tilegrid_s, bg_blur_s, median_blur_s, border_s, ], outputs=[out_img, out_txt, out_total], ) btn_reset.click(reset_count, [], [out_total]) if __name__ == "__main__": demo.launch()