try to save memory

app.py

@@ -278,7 +278,6 @@ def get_heatmaps(gene, locus1, locus2, heatmap_range):
 
 
 def perturb(gene, locus1, locus2, use_max_gene, tf_knockout):
-    in_rna = rna.copy()
     if tf_knockout:
         guidance_hvf = prior.copy()
         try:
@@ -287,8 +286,6 @@ def perturb(gene, locus1, locus2, use_max_gene, tf_knockout):
             pass
         # find index of tf and set all counts and values to zero
         gene_idx = gene_idx_map[gene]
-        in_rna.layers['counts'][:, gene_idx] = 0
-        in_rna.X[:, gene_idx] = 0
         a1 = get_closest_peak_to_gene(gene, rna, peaks)
         try:
             guidance_hvf.remove_node(a1)
@@ -367,7 +364,7 @@ def perturb(gene, locus1, locus2, use_max_gene, tf_knockout):
         except:
             pass
     
-    perterbed_rna_recon = glue.decode_data("rna", "rna", in_rna, guidance_hvf)
+    perterbed_rna_recon = glue.decode_data("rna", "rna", rna, guidance_hvf)
     ism = np.log2((perterbed_rna_recon + eps) / (rna_recon + eps))
     if tf_knockout:
         max_gene_idxs = np.abs(np.mean(ism, axis=0)).argsort()[::-1][:10]