[ { "id": "chunk0", "chunk": "激 光 生 物 学 报 ACTA LASER BIOLOGY SINICA Vol. 33 No. 5 Oct. 2024 第33 卷第5 期 2024 年10 月 收稿日期:2024-03-15", "method": "semantic_chunk", "vector": [ -0.0210746917873621, -0.01830998994410038, -0.035340938717126846, -0.007285625208169222, -0.05234237015247345, -0.018073860555887222, -0.008225229568779469, 0.07249221205711365, 0.0020538477692753077, 0.06513279676437378, 0.022058570757508278, 0.007379093673080206, -0.016273360699415207, 0.05663208290934563, -0.01982516422867775, -0.0396503284573555, -0.0253447275608778, -0.044117141515016556, -0.008348214440047741, -0.01972677744925022, -0.08032388985157013, 0.03740708529949188, -0.040378399193286896, 0.038331929594278336, 0.04128357023000717, 0.07005219161510468, 0.008259665220975876, -0.03504577651619911, 0.011255577206611633, -0.02156663127243519, 0.02111404575407505, -0.055254653096199036, 0.02739119529724121, -0.04049646481871605, 0.020012101158499718, -0.05135849118232727, -0.03274349868297577, 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Feifei2, WANG Fang2* (1. Guangxi University of Chinese Medicine, Nanning 530200, China; 2. Intensive Care Unit, Shenzhen Key Laboratory of Microbiology in Genomic Modification & Editing and Application, Shenzhen Institute of Translational Medicine, Shenzhen Second People′s Hospital, Shenzhen 518035, China) Abstract: Water pollution is one of the most serious environment problems that affect people′s life quality and sustainable economic development worldwide. The degradation capability of microorganisms endows them advantages in environmental pollutants elimination and water environments repairment, with the utilization of diverse bioremediation technologies. However, due to the complex situation of water pollution environment, traditional microbiological treatment methodology is restricted and limited. With the engineering principle Design-Build-Test-Learn in synthetic biology, the designed and modified synthetic mi- croorganisms with efficient and broad-spectrum degradation ability for pollutants has attracted the researchers′ attention. The synthetic microorganisms could not only meet the wastewater treatment requirements within complex pollutants, but also could achieve the green, efficient circular treatment goal. Therefore, design and construction of synthetic microorganisms for water pollution control has becoming one of the future development and application direction of synthetic biology. Herein, we intro- duced the current situation of water pollution, the treatment technologies classically utilized, and the synthetic microorganisms′ application in water pollution control. This review focuses on different types of microbial chassis in synthetic biology and the 419 第5 期 水是生命的起源,是生命赖以生存的根本,是 经济生产不可替代的基础,是维护生态环境的要 素", "method": "semantic_chunk", "vector": [ -0.03467245399951935, 0.0021695250179618597, -0.04210226610302925, -0.0283211637288332, -0.021370695903897285, -0.0741383358836174, -0.06483110040426254, 0.07753368467092514, 0.017166459932923317, 0.08795938640832901, -0.04929240792989731, 0.008698073215782642, 0.016297651454806328, 0.024905847385525703, 0.00585197564214468, -0.05628282204270363, -0.054565176367759705, 0.0011465527350082994, -0.01567850075662136, -0.043340567499399185, -0.0019473297288641334, -0.05108994245529175, 0.010815169662237167, -0.00801900401711464, 0.08468388020992279, 0.09115500003099442, 0.04437914490699768, -0.02059176377952099, 0.015698473900556564, -0.04298106208443642, 0.026983000338077545, -0.018944023177027702, 0.0405244305729866, -0.002455383073538542, 0.02838108316063881, -0.08963708579540253, -0.005832002963870764, 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This review will facilitate the performance optimi- zation of pollutant degradation and wastewater treatment via synthetic biology and provide reference for the efficient treatment of wastewater environment. Key words: synthetic microorganisms; microbial chassis; water pollution; bioremediation technology; environmental gover- nance (Acta Laser Biology Sinica, 2024, 33(5): 418-431) 林雅倩等:合成微生物与水污染治理应用 林雅倩等:合成微生物与水污染治理应用 激 光 生 物 学 报 420 第 33 卷 1.3 医疗废水 医疗废水主要来源于医院,其成分十分复杂, 包含各种病原微生物、抗生素、有毒有机化合物、放 射性元素和离子污染物等[13]", "method": "semantic_chunk", "vector": [ -0.014829135499894619, -0.0013926768442615867, -0.04550470784306526, -0.04163113608956337, 0.002392861060798168, -0.07911480963230133, -0.04722629487514496, 0.09742624312639236, -0.010818617418408394, 0.07981909066438675, -0.03368835151195526, 0.02770191989839077, 0.009322009049355984, 0.026058586314320564, -0.005189219024032354, -0.07363702356815338, -0.0616641603410244, -0.03302319347858429, -0.01243260595947504, -0.04112248495221138, -0.0007959900540299714, -0.04554383456707001, 0.01683439500629902, -0.005047383718192577, 0.08091464638710022, 0.09813053160905838, 0.019377650693058968, -0.04660026356577873, 0.02601945959031582, 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References E. coli BL221-AI Insertion of pheA1, pheA2, catA, catB, catC, catD, pcaI, pcaJ and pcaF Degradation of phenol [50] E. coli BL21-AI Insertion of tfdA, tfdB, tfdC, tfdD, tfdE, tfdF, pcaI, pcaJ and pcaF Degradation of 2, 4-D [51] E. coli BL21-AI Insertion of DntAa, DntAb, DntAc, DntAd, DntB, DntD, DntG and DntE Degradation of 2, 4-DNT [106] E. coli TOP10 Insertion of the salicylicacid (SA) detection, degradation, and suicide pathway genes Pfic‐TAT‐P1XX‐RBS Detection and degradation of SA [109] E. coli TOP10 Insertion of the Cd sensing pathway gene pPcad-ind Detection of Cd [113] E. coli TOP10 Insertion of the Hg and Cd sensory elements and the separate reporters pR-Pcad-Pmer-G Enhanced detection of Hg and Cd [114] P. putida KT2440 Insertion of mpd, pnpA, pnpB, gfp, linA, linB, linC, linD and vgb Degradation of MP and γ-HCH [58] P. putida KT2440 Deletion of GIs, insertion of the degradation pathway genes of γ-HCH and TCP Enhanced degradation of γ-HCH and TCP [59] P. putida KT2440 Deletion of hsdRMS, insertion of linA, linB, linC, linD, dhaA31, hheC and echA Enhanced degradation of γ-HCH and TCP [105] P. putida KT2440 Insertion of mpd, pytH, mcd, cehA, gfp and vgb Degradation of multiple pesticides [107] B. subtilis Design and Insertion of metal sensor modules MerRZntR Detection of heavy metal ions [61] B. subtilis ZN0871v11 Deletion of yqfY and spo0A Adsorption of organic molecules and ions [63] B. subtilis N4 Insertion of nha and ami Degradation of nitriles [67] B. subtilis 1935 Deletion of epsA~O and bslA, and expression of mtagBFP, eGFP, mCherry and TasA-MT Detection and adsorption of mul- tiple heavy metal ions [68] S. cerevisiae Insertion of CTR1 and CUP1 Detection of Cu [74] S. cerevisiae W303a Insertion of JLP1-lacZ Detection of Cd [75] S. cerevisiae BY4741 Insertion of myrGFP-MTx Adsorption of heavy metal ions (Cu, Ni and Mn) [76] S. cerevisiae EBY100 Insertion of 4R-PdBP and aga2, overexpression of aga1 Adsorption of Pd [77] H. Bluephagenesi WZY278 Overexpression of phaCABCn and linkage with ompW Recovery of food wastes [87] Halomonas sp. EF43 Insertion of the 2, 4-D, MCPA and 3-chlorobenzoate acid degradation pathway genes Degradation of 2, 4-D and 3-chlorobenzoate acid [88] Acinetobacter sp. ADP1 Insertion of luxCDABE Detection of multiple metal ions [93] Acinetobacter sp. 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