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README.md

@@ -1,12 +1,118 @@
----
-title: Crispr Gpt
-emoji: 🐢
-colorFrom: pink
-colorTo: yellow
-sdk: gradio
-sdk_version: 5.43.1
-app_file: app.py
-pinned: false
----
+<div align="center">
+
+# CRISPR-GPT for agentic automation of gene-editing experiments
+
+<div>
+🧬 Multi-Agent System for Automated CRISPR Experiment Design and Analysis 🔬
+</div>
+</div>
+
+<br>
+<div align="center">
+<!-- Buttons -->
+<a href="https://www.nature.com/articles/s41551-025-01463-z" style="text-decoration:none;">
+  <img src="https://img.shields.io/badge/Paper-Nature%20BME-red?style=for-the-badge&logo=readthedocs&logoColor=white" 
+       style="height:30px; margin:10px;">
+<br>
+
+<a href="https://genomics.stanford.edu" style="text-decoration:none;">
+  <img src="https://img.shields.io/badge/Website-genomics.stanford.edu-blue?style=for-the-badge&logo=google-chrome&logoColor=white" 
+       style="height:30px; margin:10px;">
+<br>
+</a>
+<br>
+<p style="font-size:20px; font-weight:bold; margin-top:20px;">
+  Please sign-up for beta access to CRISPR-GPT:
+</p>
+<a href="https://www.surveymonkey.com/r/G9GCMJV" style="text-decoration:none;">
+  <img src="https://img.shields.io/badge/Early%20Access-Sign%20Up-green?style=for-the-badge&logo=check-circle&logoColor=white" 
+       style="height:30px; margin:10px;">
+</a>
+</div>
+
+</div>
+
+## Overview
+
+<p align="center">
+<img width="600" alt="CRISPR-GPT Overview" src="assets/fig1.png">
+</p>
+
+CRISPR-GPT is an innovative Large Language Model (LLM) agent designed to automate and streamline the process of designing gene-editing experiments. By leveraging the power of advanced language models, CRISPR-GPT assists researchers in planning, executing, and analyzing CRISPR-based gene editing tasks with unprecedented efficiency and accuracy.
+
+CRISPR-GPT supports four primary gene-editing modalities: **knockout**, **base-editing**, **prime-editing**, and **epigenetic editing**. The system automates 22 individual tasks including CRISPR/Cas system selection, sgRNA design, off-target prediction, delivery method optimization, and experimental validation protocols. Users can interact with CRISPR-GPT through three distinct modes: (1) **Meta mode** for step-by-step guidance on pre-defined gene-editing workflows, (2) **Auto mode** for customized guidance on free-style user requests, and (3) **QA mode** for real-time answers to ad hoc questions. These modes accommodate different user expertise levels and experimental requirements, from novice researchers seeking structured guidance to experienced scientists needing flexible, custom workflows or quick troubleshooting support.
+
+**Related Work**: This project builds upon our [Genome-Bench](https://github.com/mingyin0312/RL4GenomeBench) research, which developed RL fine-tuning methods to inject expert knowledge from 10+ years of genomics discussions into language models.
+
+## Architecture
+
+<img width="830" alt="CRISPR-GPT Architecture" src="assets/fig2.png">
+
+The backbone of CRISPR-GPT involves multi-agent collaboration between four core components:
+
+1. **LLM Planner Agent**: Configures tasks and performs automatic task decomposition and planning based on user requests
+2. **Task Executor Agent**: Implements the chain of state machines from the Planner Agent and manages workflow execution
+3. **LLM User-Proxy Agent**: Interfaces with the Task Executor on behalf of users, enabling monitoring and corrections
+4. **Tool Providers**: Support diverse external tools and connect to search engines or databases via API calls
+
+## Getting Started
+
+### Release Information
 
-Check out the configuration reference at https://huggingface.co/docs/hub/spaces-config-reference
+Due to safety concerns regarding AI applications in biological research, the complete CRISPR-GPT codebase (including full data, code, and prompts) will not be publicly released until the development of comprehensive US regulations governing artificial intelligence and its scientific applications.
+
+While the full code is not freely available, **it has undergone rigorous peer review** as part of our Nature Biomedical Engineering publication process.
+
+### Light Version (Developer Preview)
+
+We are releasing a **light version** of CRISPR-GPT on GitHub that includes:
+
+- ✅ Core infrastructure and framework
+- ✅ Basic chat interface
+- ✅ Limited task/functionality support
+
+### Installation (Light Version)
+
+```bash
+# Clone the repository
+git clone https://github.com/cong-lab/crispr-gpt-pub.git
+cd crispr-gpt-pub
+
+# Create conda environment
+conda create -n crispr-gpt-new python=3.11 -y
+conda activate crispr-gpt-new
+
+# Install dependencies
+pip install -r requirements.txt
+
+# Set up OpenAI API key
+echo "OPENAI_KEY=your_openai_api_key_here" > .env
+
+# Run the application
+python main.py
+```
+
+## Citation
+
+```bibtex
+@article{qu2025crisprGPT,
+  title={CRISPR-GPT for agentic automation of gene-editing experiments},
+  author={Qu, Yuanhao and Huang, Kaixuan and Yin, Ming and others},
+  journal={Nature Biomedical Engineering},
+  year={2025},
+  doi={10.1038/s41551-025-01463-z},
+  url={https://doi.org/10.1038/s41551-025-01463-z}
+}
+```
+
+## Contact
+
+For questions, suggestions, or collaborations, please contact:
+
+**CRISPR-GPT Team**:
+- Yuanhao Qu: [yhqu@stanford.edu](mailto:yhqu@stanford.edu)  
+- Kaixuan Huang: [kaixuanh@princeton.edu](mailto:kaixuanh@princeton.edu)
+- Mengdi Wang: [mengdiw@princeton.edu](mailto:mengdiw@princeton.edu)
+- Le Cong: [congle@stanford.edu](mailto:congle@stanford.edu)
+
+---

crisprgpt/act_rep.py

@@ -0,0 +1,14 @@
+from .logic import BaseState, Result_ProcessUserInput, BaseUserInputState
+ 
+from util import get_logger
+
+logger = get_logger(__name__)
+
+ERROR_MESSAGE="CRISPR-GPT-Lite does not support this function. "
+
+class StateError(BaseState):
+    request_user_input = False
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(response=ERROR_MESSAGE), None

crisprgpt/apis/parse_sgRNA_library.py

@@ -0,0 +1,163 @@
+from util import get_logger
+import pandas as pd
+from llm import OpenAIChat
+import uuid
+import os
+
+logger = get_logger(__name__)
+
+
+class sgRNALibraryReader:
+    def __init__(self):
+        self.ROOT = "resources/"
+        self.library = {}
+
+    def _process_txt(self, filename):
+        if filename not in self.library:
+
+            if not os.path.exists(self.ROOT + "/" + filename):
+                self.library[filename] = []
+                return
+
+            self.library[filename] = pd.read_csv(
+                self.ROOT + "/" + filename, delimiter="\t"
+            )
+ 
+    def parse_knockout_library(self, system, species):
+        f_sys = {"SpCas9": "SP", "SaCas9": "SA", "enCas12a": "EN"}.get(system, "")
+        f_spec = {"human": "human", "mouse": "mouse"}.get(species, "")
+        filename = f"KO_{f_sys}_{f_spec}.txt"
+        return self.library[filename]
+
+
+
+sgRNA_library_reader = sgRNALibraryReader()
+
+filenames = [
+    "KO_EN_human.txt",
+    "KO_EN_mouse.txt",
+    "KO_SA_human.txt",
+    "KO_SA_mouse.txt",
+    "KO_SP_human.txt",
+    "KO_SP_mouse.txt",
+]
+
+for filename in filenames:
+    sgRNA_library_reader._process_txt(filename)
+
+
+def subset_value(df, column_name, values):
+    """
+    Subset the rows where the specified column matches any of the given values.
+
+    Parameters:
+    df (pd.DataFrame): The DataFrame to subset.
+    column_name (str): The name of the column to match.
+    values (list): A list of values to match in the specified column.
+
+    Returns:
+    pd.DataFrame: A subset of the original DataFrame with rows where the column matches any of the values.
+    """
+    temp_column = df[column_name].astype(str).str.lower()
+    values = [val.lower() for val in values]
+    return df[temp_column.isin(values)]
+
+
+def sort_table(df, column_name, ascending=True):
+    """
+    Sort the DataFrame based on values in the specified column.
+
+    Parameters:
+    df (pd.DataFrame): The DataFrame to sort.
+    column_name (str): The name of the column to sort by.
+    ascending (bool): Whether to sort in ascending order (default is True).
+
+    Returns:
+    pd.DataFrame: The sorted DataFrame.
+    """
+    return df.sort_values(by=column_name, ascending=ascending)
+
+
+def get_top_n_rows(df, n):
+    """
+    Get the top N rows of the DataFrame.
+
+    Parameters:
+    df (pd.DataFrame): The DataFrame to get the top N rows from.
+    n (int): The number of top rows to return.
+
+    Returns:
+    pd.DataFrame: The top N rows of the DataFrame.
+    """
+    return df.head(n)
+
+
+def subset_between(df, column_name, x=None, y=None):
+    """
+    Subset the rows where the specified column's values are between x and y (inclusive).
+    If x is None, it subsets only based on y. If y is None, it subsets only based on x.
+
+    Parameters:
+    df (pd.DataFrame): The DataFrame to subset.
+    column_name (str): The name of the column to match.
+    x (str or int or float, optional): The lower bound value. Defaults to None.
+    y (str or int or float, optional): The upper bound value. Defaults to None.
+
+    Returns:
+    pd.DataFrame: A subset of the original DataFrame with rows where the column's values are between x and y.
+    """
+    if x is not None and y is not None:
+        return df[(df[column_name] >= x) & (df[column_name] <= y)]
+    elif x is not None:
+        return df[df[column_name] >= x]
+    elif y is not None:
+        return df[df[column_name] <= y]
+    else:
+        raise ValueError("At least one of x or y must be provided.")
+
+
+def extract_info(user_input, prompt_template, df):
+    try:
+        dataframe = df
+        prompt = prompt_template.format(user_message=user_input)
+        print(prompt)
+        result = OpenAIChat.chat(prompt, use_GPT4_turbo=True)
+        print(result)
+        for item in result["Actions"]:
+            function = item["called_function"]
+            print(function)
+            if function == "subset_value":
+                column_name = item["column_name"]
+                values = [val.strip() for val in item["matching_value"].split(",")]
+                dataframe = subset_value(dataframe, column_name, values)
+            if function == "sort":
+                column_name = item["column_name"]
+                ascending = item["ascending"]
+                if ascending == "TRUE":
+                    dataframe = sort_table(dataframe, column_name, ascending=True)
+                else:
+                    dataframe = sort_table(dataframe, column_name, ascending=False)
+            if function == "get":
+                num_rows = dataframe.shape[0]
+                n = int(item["n"])
+                print(n)
+                if num_rows == 0:
+                    print("error")
+                if num_rows < n:
+                    n = num_rows
+                dataframe = get_top_n_rows(dataframe, n)
+            if function == "subset_between":
+                column_name = item["column_name"]
+                x = None
+                y = None
+                if x != "NA":
+                    x = int(item["x"])
+                if y != "NA":
+                    y = int(item["y"])
+                dataframe = subset_between(df, column_name, x=x, y=y)
+        return dataframe, ""
+    except Exception as ex:
+        print(ex)
+        print("could not extract relevant info")
+        dataframe = pd.DataFrame()
+        return dataframe, ""

crisprgpt/automation.py

@@ -0,0 +1,194 @@
+from .logic import BaseState, Result_ProcessUserInput, BaseUserInputState
+from llm import OpenAIChat, IdentifiableGeneError
+from . import base_editing, knockout, prime_editing, act_rep, off_target
+from .logic import StateFinal, gradio_state_machine, EmptyState, EmptyStateFinal
+
+from dataclasses import dataclass
+from typing import Optional, List
+from util import get_logger
+logger = get_logger(__name__)
+
+@dataclass
+class ExecutorState:
+    user_prompt: Optional[str] = None
+    cached_message: Optional[List] = None  
+    flag_user_ack: bool = False 
+    cached_user_input: Optional[str] = None
+
+
+
+PROMPT_PROCESS_AUTOMATE = """
+Please act as an expert in CRISPR technology. Given the user input, think step by step and generate a list of tasks for execution. First refer to the task description table below, and try to figure out if the user needs to directly jump into a task, or the user needs to complete several tasks. Make sure to respect the task notes.
+
+Please do not include unnecessary steps and there are no dependencies within the steps. Please make sure the generated list of tasks are in the order indicated in the task description table. Please format your response and make sure it is parsable by JSON.
+
+## Task Description Table
+
+For knockout
+
+task name: task descriptions: notes
+knockout.StateStep1: Cas System selection for knockout : none
+knockout.StateStep3: guideRNA design for knockout : none
+
+For base editing
+
+task name: task descriptions: notes
+base_editing.StateError: the error-handling state for all tasks about base editing : CRISPR-GPT-Lite version does not support base editing. 
+
+For prime editing
+
+task name: task descriptions: notes
+prime_editing.StateError: the error-handling state for all tasks about prime editing : CRISPR-GPT-Lite version does not support prime editing. 
+
+For CRISPRa/CRISPRi
+
+task name: task descriptions: notes
+act_rep.StateError: the error-handling state for all tasks about CRISPRa/CRISPRi : CRISPR-GPT-Lite version does not support CRISPRa/CRISPRi. 
+
+For Off-Target Prediction
+
+task name: task descriptions: dependency
+off_target.OffTarget: Off-target search/prediction using CRISPRitz: none
+
+## Demonstrations:
+If the user only needs to design guideRNA for knockout, then return ['knockout.StateStep3']. Reason: this directly matches knockout.StateStep3.
+
+
+User Input:
+
+{user_message}
+
+Response format:
+
+{{
+"Thoughts": "<thoughts>",
+"Tasks": ["<task1>", "<task2>"]  ## a list of task names 
+}}"""
+
+class StateAutomate(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_AUTOMATE
+    request_message = "Please directly type in your need."
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message = user_message) 
+        response = OpenAIChat.chat(prompt, use_GPT4=True)
+        tasks = response['Tasks']
+        logger.info(tasks)
+        tasks = [eval(x) for x in tasks]
+        tasks.insert(0, EmptyState)
+        tasks.append(EmptyStateFinal) 
+        executor = gradio_state_machine(task_list=tasks) 
+        kwargs['memory']['executor'] = executor
+        executor.memory['flag_human_heritable_editing_ack'] = True  
+        kwargs['memory']['executor_state'] = ExecutorState(user_prompt=user_message, cached_message=[], flag_user_ack = True)
+
+        return Result_ProcessUserInput(status='success', thoughts= response['Thoughts'] , result=user_message, response=str(response)),  StateAutomateStep
+
+class StateAutomateYesNo(BaseUserInputState):  
+    
+    @classmethod
+    def step(cls, user_message, **kwargs): 
+        if user_message.strip() != "":
+            kwargs['memory']['executor_state'].cached_user_input = user_message
+        return Result_ProcessUserInput(), StateAutomateStep
+
+
+class StateAutomateStep(BaseState):  
+    @classmethod
+    def FallbackState(cls):
+        return StateFinal
+    
+    @classmethod
+    def step(cls, user_message, **kwargs):  
+        executor = kwargs['memory']['executor']
+        executor_state = kwargs['memory']['executor_state']
+        user_message = executor_state.cached_user_input
+        cached_message = executor_state.cached_message
+        meta_prompt = executor_state.user_prompt
+
+
+        if len(cached_message) > 0: 
+            # display message one by one
+            executor_state.flag_user_ack = False
+            return Result_ProcessUserInput(response=cached_message.pop(0)), StateAutomateStep
+        else: 
+            if executor_state.flag_user_ack: 
+                # we've got user acknowledgement, loop and get to next state.
+
+                list_bot_message = executor.loop(user_message, email=kwargs["email"])
+                executor_state.cached_message = list_bot_message[:]
+
+                if not executor.current_state.isFinal:   
+                    generated_response = cls.gen_response(meta_prompt=meta_prompt, system_message=list_bot_message)
+                    generated_answer = str(generated_response['Answer'])
+                    generated_rational = generated_response['Thoughts']
+
+                    executor_state.cached_user_input = generated_answer
+                    generated_answer = "Here is the GPT-suggested answer: \n\n" + generated_answer + '\n\n\n Reason: \n' + generated_rational
+                    executor_state.cached_message.append(generated_answer)  
+
+                executor_state.flag_user_ack = False
+                return Result_ProcessUserInput(), StateAutomateStep
+            else:
+                if not executor.current_state.isFinal:  
+                    # Send to user for acknowledgement.
+                    executor_state.flag_user_ack = True
+                    return Result_ProcessUserInput(response=RESPONSE_CHECK_USER), StateAutomateYesNo
+                else:
+                    return Result_ProcessUserInput(), StateFinal
+
+                
+                
+    @classmethod
+    def gen_response(cls, meta_prompt, system_message):  
+        system_message = [msg for msg in system_message if isinstance(msg, str)]
+        system_message = '\n\n'.join(system_message)
+        try:
+            prompt = PROMPT_GENERATE_USER_RESPONSE.format(meta_prompt= meta_prompt, system_message = system_message)
+            logger.info(prompt)
+            response = OpenAIChat.chat(prompt, use_GPT4=True) 
+            logger.info(response)
+            return response 
+        except IdentifiableGeneError as ex:
+            logger.info(["Not supported in Automated Mode"])
+            return dict(Thoughts="Input/Output may contain sensitive sequences. This is not supported in Automation mode! Please manually enter your response!", Answer="I don't know")
+        except Exception as ex:
+            logger.info(["Error occured", ex]) 
+            return dict(Thoughts="error occured" + ex + "Please manually enter your response!", Answer="I don't know")
+
+
+
+RESPONSE_CHECK_USER = "Press Enter to continue with the generated answer. Enter other text to correct answer"
+
+ 
+
+PROMPT_GENERATE_USER_RESPONSE="""
+Please act as you are using the CRISPR design tool. Given the user meta request, the current inquiry provided by the tool, think step by step and generate an answer to the questions. Please format your response and make sure it is parsable by JSON.
+
+Rules:
+
+1. Answer the inquiry directly on behalf of the user. Don't raise any additional question to the user.
+2. If the inquiry is a multiple-choice question, then directly output one choice. 
+3. If the inquiry asks you to supply any gene sequence, then answer the question with "I don't know" and let the user take manual control.
+
+User Meta Request:
+
+"{meta_prompt}"
+
+Current Inquiry:
+
+"{system_message}"
+
+Response format:
+
+{{
+"Thoughts": "<thoughts>",
+"Answer": "<response string>"
+}}"""
+ 
+
+class AutomationEntryState(BaseState):
+    @classmethod 
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(), StateAutomate

crisprgpt/base_editing.py

@@ -0,0 +1,14 @@
+from .logic import BaseState, Result_ProcessUserInput, BaseUserInputState
+ 
+from util import get_logger
+
+logger = get_logger(__name__)
+
+ERROR_MESSAGE="CRISPR-GPT-Lite does not support this function. "
+
+class StateError(BaseState):
+    request_user_input = False
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(response=ERROR_MESSAGE), None

crisprgpt/entry.py

@@ -0,0 +1,155 @@
+from .logic import BaseState, Result_ProcessUserInput, BaseUserInputState, StateFinal
+from llm import OpenAIChat
+from . import base_editing, knockout, prime_editing, act_rep
+from .automation import StateAutomate
+
+PROMPT_REQUEST_ENTRY = """Welcome to CRISPR-GPT. I can help with the following tasks. Please select 
+one to continue.
+
+1. Meta-Mode (Step-by-Step Guidance on Pre-defined Meta-Task)
+2. Auto-Mode (Customized Guidance on Free-style User Request)
+
+Note: Chat with ChatGPT augmented with our up-to-date knowledge base 
+directly by entering Questions followed by "Q:". For example, "Q: What is 
+CRISPR?"
+"""
+
+PROMPT_PROCESS_ENTRY = """
+Please act as an expert in CRISPR technology. Given the user instruction and user input, think step by step and generate a choice for the user. Please format your response and make sure it is parsable by JSON.
+
+User Instructions:
+
+"Welcome to CRISPR-GPT. I can help with the following tasks. Please select 
+one to continue.
+
+1. Meta-Mode (Step-by-Step Guidance on Pre-defined Meta-Task)
+2. Auto-Mode (Customized Guidance on Free-style User Request)
+
+Note: Chat with ChatGPT augmented with our up-to-date knowledge base 
+directly by entering Questions followed by "Q:". For example, "Q: What is 
+CRISPR?"
+"
+
+User Input:
+
+"{user_message}"
+
+Response format:
+
+{{
+"Thoughts": "<thoughts>",
+"Choice": "<choice>"  ## output number only
+}}"""
+
+
+class EntryState(BaseState):
+    request_user_input = False
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(response=PROMPT_REQUEST_ENTRY), EntryStateChoice
+
+
+class EntryStateChoice(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_ENTRY
+
+    @classmethod
+    def NextState(cls, choice):
+        if choice.lower() in ["1", "i", "(1)", "(i)"]:
+            return MetaStateEntry
+        elif choice.lower() in ["2", "ii", "(2)", "(ii)"]:
+            return StateAutomate
+        else:
+            return cls
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message=user_message)
+        response = OpenAIChat.chat(prompt)
+        return Result_ProcessUserInput(
+            status="success",
+            thoughts=response["Thoughts"],
+            result=response["Choice"],
+            response=str(response),
+        ), cls.NextState(response["Choice"])
+
+
+PROMPT_REQUEST_META = """Please select the general gene editing scenarios to continue.
+1. Generating a Knockout Using CRISPR.
+2. CRISPR Base Editing Without Double-Strand Breaks. (Not Supported in Lite version)
+3. Generating Small Insertion/deletion/base editing through Prime Editing. (Not Supported in Lite version)
+4. Activation or Repression of Target Genes Using CRISPR. (Not Supported in Lite version)
+"""
+
+PROMPT_PROCESS_META = """
+Please act as an expert in CRISPR technology. Given the user instruction and user input, think step by step and generate a choice for the user. Please format your response and make sure it is parsable by JSON.
+
+User Instructions:
+
+"Please select the general gene editing scenarios to continue.
+1. Generating a Knockout Using CRISPR.
+2. CRISPR Base Editing Without Double-Strand Breaks.
+3. Generating Small Insertion/deletion/base editing through Prime Editing.
+4. Activation or Repression of Target Genes Using CRISPR.
+"
+
+User Input:
+
+"{user_message}"
+
+Response format:
+
+{{
+"Thoughts": "<thoughts>",
+"Choice": "<choice>"  ## output number only
+}}"""
+
+
+class MetaStateEntry(BaseState):
+    request_user_input = False
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(response=PROMPT_REQUEST_META), MetaStateChoice
+
+
+class MetaStateChoice(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_META
+
+    @classmethod
+    def NextState(cls, choice):
+        if choice.lower() in ["1", "i", "(1)", "(i)"]:
+            return [
+                knockout.StateEntry,
+                knockout.StateStep1, 
+                knockout.StateStep3, 
+                StateFinal,
+            ]
+        elif choice.lower() in ["2", "ii", "(2)", "(ii)"]:
+            return [
+                base_editing.StateError,
+                StateFinal,
+            ]
+        elif choice.lower() in ["3", "iii", "(3)", "(iii)"]:
+            return [
+                prime_editing.StateError,
+                StateFinal,
+            ]
+        elif choice.lower() in ["4", "iv", "(4)", "(iv)"]:
+            return [
+                act_rep.StateError,
+                StateFinal,
+            ]
+        else:
+            return cls
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message=user_message)
+        response = OpenAIChat.chat(prompt)
+        return Result_ProcessUserInput(
+            status="success",
+            thoughts=response["Thoughts"],
+            result=response["Choice"],
+            response=str(response),
+        ), cls.NextState(response["Choice"])

crisprgpt/knockout.py

@@ -0,0 +1,91 @@
+from .logic import BaseState, Result_ProcessUserInput, BaseUserInputState
+from llm import OpenAIChat
+from .knockout_constant import *
+from .off_target import OffTarget
+from .knockout_sgRNA_selection import SgRNASelection
+import numpy
+import re
+import requests
+import os
+from util import get_logger
+
+logger = get_logger(__name__)
+
+
+class StateEntry(BaseState):
+    request_user_input = False
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(response=PROMPT_REQUEST_ENTRY), None
+
+
+class StateStep1(BaseState):
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(response=PROMPT_STEP1), StateStep1Inquiry
+
+
+class StateStep1Inquiry(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_STEP1_INQUIRY
+    request_message = PROMPT_REQUEST_STEP1_INQUIRY
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message=user_message)
+        response = OpenAIChat.chat(prompt, use_GPT4=True)
+        text_response = str(response)
+        result = response["Answer"]
+        text_response += f" Final Result {result}"
+        return (
+            Result_ProcessUserInput(
+                status="success",
+                thoughts=response["Thoughts"],
+                result=result,
+                response=text_response,
+            ),
+            None,
+        )
+
+
+
+class StateStep1Easy(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_EASY
+    request_message = PROMPT_REQUEST_EASY
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message=user_message)
+        response = OpenAIChat.chat(prompt)
+        text_response = str(response)
+        result = response["Answer"]
+        text_response += f" Final Result {result}"
+        return (
+            Result_ProcessUserInput(
+                status="success",
+                thoughts=response["Thoughts"],
+                result=result,
+                response=text_response,
+            ),
+            StateStep3,
+        )
+
+
+class StateStep3(BaseState):
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        if kwargs["is_automation"]:
+            if "StateStep1Easy" in kwargs["memory"]:
+                return Result_ProcessUserInput(), SgRNASelection
+            else:
+                return (
+                    Result_ProcessUserInput(response=PROMPT_REQUEST_STEP3),
+                    StateStep1Easy,
+                )
+        else:
+            return (
+                Result_ProcessUserInput(response=PROMPT_REQUEST_STEP3),
+                SgRNASelection,
+            )
+
+ 

crisprgpt/knockout_constant.py

@@ -0,0 +1,166 @@
+PROMPT_REQUEST_EASY = """
+In order to complete the current task, we would need you to answer the following questions. 
+
+If you are not sure how to select, we recommend you use the Meta-mode where we could generate case-specific guidance based on your needs, please open a new chat and enter meta-mode (select option 1 for the initial prompt).
+
+1. Which Cas system are you planning to use? (We currently support SpCas9, enCas12a, SaCas9, SpRYCas9)
+"""
+
+PROMPT_PROCESS_EASY = """Please act as an expert in CRISPR technology. Given the instruction and user input, think step by step and give the response. Please format your response following response format and make sure it is parsable by JSON.
+
+Instruction:
+
+Given the user input, identify the single most relevant Cas system below.
+
+1. SpCas9 (from Streptococcus pyogenes)
+Pros:
+High efficiency in a wide range of organisms.
+Extensive research and documentation available.
+Cons:
+Large protein size can complicate delivery mechanisms.
+Potential for off-target effects.
+Applications:
+Broad, including basic genetic research, gene therapy, and development of genetically modified organisms (GMOs).
+
+2. enCas12a (engineered Cas12a variant)
+Pros:
+Smaller than SpCas9, allowing for easier delivery in certain vectors.
+Generates staggered DNA double-strand breaks, potentially facilitating precise genome editing.
+Has a lower off-target rate, offering increased genome editing fidelity.
+Unique ability to simultaneously cut multiple sites, allowing for the efficient targeting and editing of several genomic locations in a single experiment.
+Cons:
+Sometimes exhibits lower editing efficiency compared to SpCas9.
+Requires distinct PAM sequences, which might limit target sites.
+Applications:
+Ideal for applications requiring higher precision, reduced off-target and multiplexed editing, like therapeutic gene editing and complex genome engineering tasks.
+
+3. SaCas9 (from Staphylococcus aureus)
+Pros:
+More compact than SpCas9, enhancing its suitability for certain delivery methods such as AAV vectors.
+Cons:
+Limited by a narrower PAM compatibility, restricting its targeting scope.
+Applications:
+Primarily used in in vivo therapeutic applications where delivery vector size is a limiting factor, such as gene therapy for inherited genetic disorders.
+
+4. SpRYCas9 (engineered variant of SpCas9)
+Pros:
+Broadened PAM recognition, greatly expanding genomic targeting capabilities.
+Cons:
+Increased potential for off-target effects due to broader specificity.
+Applications:
+Suitable for complex genome editing applications targeting genomic regions previously inaccessible, including research and therapeutic contexts where high specificity is not as critical.
+
+User Input:
+
+{user_message}
+
+Response format:
+{{
+"Thoughts": "<thoughts>", # Think step by step to figure out which Cas system is most relevant to user's input.
+"Answer": "<answer>" # Choose the single most relevant Cas system. Don't put additional words here. Only put a single name (SpCas9, enCas12a, SaCas9, SpRYCas9).
+}}"""
+
+
+
+PROMPT_REQUEST_ENTRY = """Now, let's start designing and planning your knockout experiment using CRISPR system, we will go through a step by step process as listed below:
+1. Selecting a CRISPR/Cas system.
+2. Selecting a delivery approach for CRISPR/Cas system. (Not Supported in Lite version)
+3. Designing guideRNA for gene knockout.
+4. Collecting appropriate experimantal protocols and validating the editing outcomes. (Not Supported in Lite version)
+"""
+
+PROMPT_STEP1 = """
+Step 1. Selecting a CRISPR/Cas system 
+
+We support four types of CRISPR/Cas system for CRISPR knockout, SpCas9, enCas12a, SaCas9, and SpRYCas9, representing distinct variants with unique properties and applications.
+
+1. SpCas9  (Jinek et al.,Science,2012;Cong et al.,Science,2013;Mali et al.,Science,2013;Jinek et al.,eLife,2013)
+- Pros: Highly effective, well-studied, proven applications in many cell types/scenarios.  
+- Cons: Large, potential off-target effects.  
+- Uses: Genetic research, gene therapy, genetically modified models/organisms (GMMs/GMOs).
+
+2. enCas12a  (Kleinstiver et al.,Nat Biotechnol.,2019;DeWeirdt et al.,Nat Biotechnol.,2020)
+- Pros: Smaller, precise, low off-target rate, multi-target editing capability using a single guideRNA array.  
+- Cons: May be less efficient than SpCas9 when using a single guideRNA (mitigated if using multi-guide array), limited target sites vs. SpCas9 due to more restrictive PAM.  
+- Uses: High-precision and multiplexed genome editing (for genetic interaction, target redundant genes/pathways).
+
+3. SaCas9  (Ran et al.,Nature,2015)
+- Pros: Compact, suitable for AAV vectors (knockout genes in primary cells or in vivo).  
+- Cons: limited target sites vs. SpCas9 due to more restrictive PAM.  
+- Uses: In vivo gene therapy and some primary cells when using AAV delivery.
+
+4. SpRYCas9  (Walton et al.,Science,2020)
+- Pros: Wide PAM range, targets more genomic regions vs. typical SpCas9.  
+- Cons: Higher off-target risks.  
+- Uses: editing a specific target site where canonical PAMs for SpCas9/SaCas9/enCas12a are not available, and where specificity/off-target concerns are less critical.
+
+*Please cite the corresponding papers if you decide to use the system.
+"""
+
+PROMPT_REQUEST_STEP1_INQUIRY = """
+Please select the CRISPR-Cas system you would like to use. Or please briefly describe your need (exp: I want to edit mouse liver/I want low off-target rate/I want to do multiplexed editing) and we could recommend the CRSIPR-Cas system to you.
+"""
+
+PROMPT_PROCESS_STEP1_INQUIRY = """Please act as an expert in CRISPR technology. Given the instruction and user input, think step by step and give the response. Please format your response following response format and make sure it is parsable by JSON.
+
+Instruction:
+
+Given the user input, identify the single most relevant Cas system below.
+
+1. SpCas9 (from Streptococcus pyogenes)
+Pros:
+High efficiency in a wide range of organisms.
+Extensive research and documentation available.
+Cons:
+Large protein size can complicate delivery mechanisms.
+Potential for off-target effects.
+Applications:
+Broad, including basic genetic research, gene therapy, and development of genetically modified organisms (GMOs).
+
+2. enCas12a (engineered Cas12a variant)
+Pros:
+Smaller than SpCas9, allowing for easier delivery in certain vectors.
+Generates staggered DNA double-strand breaks, potentially facilitating precise genome editing.
+Has a lower off-target rate, offering increased genome editing fidelity.
+Unique ability to simultaneously cut multiple sites, allowing for the efficient targeting and editing of several genomic locations in a single experiment.
+Cons:
+Sometimes exhibits lower editing efficiency compared to SpCas9.
+Requires distinct PAM sequences, which might limit target sites.
+Applications:
+Ideal for applications requiring higher precision, reduced off-target and multiplexed editing, like therapeutic gene editing and complex genome engineering tasks.
+
+3. SaCas9 (from Staphylococcus aureus)
+Pros:
+More compact than SpCas9, enhancing its suitability for certain delivery methods such as AAV vectors.
+Cons:
+Limited by a narrower PAM compatibility, restricting its targeting scope.
+Applications:
+Primarily used in in vivo therapeutic applications where delivery vector size is a limiting factor, such as gene therapy for inherited genetic disorders.
+
+4. SpRYCas9 (engineered variant of SpCas9)
+Pros:
+Broadened PAM recognition, greatly expanding genomic targeting capabilities.
+Cons:
+Increased potential for off-target effects due to broader specificity.
+Applications:
+Suitable for complex genome editing applications targeting genomic regions previously inaccessible, including research and therapeutic contexts where high specificity is not as critical.
+
+User Input:
+
+{user_message}
+
+Response format:
+{{
+"Thoughts": "<thoughts>", # Think step by step to figure out which Cas system is most relevant to user's input.
+"Answer": "<answer>" # Choose the single most relevant Cas system. Don't put additional words here. Only put a single name (SpCas9, enCas12a, SaCas9, SpRYCas9).
+}}"""
+
+
+PROMPT_REQUEST_STEP3="""
+Step 3. Designing guideRNA for gene knockout
+
+Next, we would like to guide you through the design of guideRNA for your target gene. Designing an effective and specific single-guide RNA (sgRNA) is crucial for successful CRISPR/Cas9-mediated genome editing.There are several factors to consider in this process, including the selection of target sites that are unique to your gene of interest and minimizing potential off-target effects. 
+
+"""
+ 
+ 

crisprgpt/knockout_sgRNA_selection.py

@@ -0,0 +1,200 @@
+import pandas as pd
+from .knockout_sgRNA_selection_constant import *
+from .logic import BaseState, Result_ProcessUserInput, BaseUserInputState
+from llm import OpenAIChat
+from .off_target import OffTarget
+from .apis.parse_sgRNA_library import extract_info, sgRNA_library_reader
+import time
+ 
+
+class SgRNASelection(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_AGENT1
+    request_message = PROMPT_REQUEST_AGENT1
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message=user_message)
+        response = OpenAIChat.chat(prompt, use_GPT4=True)
+        response["original_request"] = user_message
+        if response["Specified"].lower() == "yes":
+            return (
+                Result_ProcessUserInput(
+                    status="success",
+                    result=response,
+                ),
+                StateStepOriginal,
+            )
+        else:
+            text_response = "Sugessted exon/exons: " + str(
+                {
+                    "Exon/exons": response["target exon"],
+                    "Reason": {response["rationale"]},
+                }
+            )
+            return (
+                Result_ProcessUserInput(
+                    status="success",
+                    result=response,
+                    response=text_response,
+                ),
+                StateStepQuestion,
+            )
+
+
+class StateStepQuestion(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_QUESTION
+    request_message = PROMPT_REQUEST_QUESTION
+    prev_state_name = "SgRNASelection"
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prev_result = kwargs["memory"][cls.prev_state_name].result
+        target_exon = prev_result["target exon"]
+        rationale = prev_result["rationale"]
+        prompt = cls.prompt_process.format(
+            user_message=user_message, target_exon=target_exon, rationale=rationale
+        )
+        response = OpenAIChat.chat(prompt, use_GPT4=True)
+        choice = response["Choice"]
+        if choice.lower() == "yes":
+            return (
+                Result_ProcessUserInput(
+                    status="success", thoughts=response["Thoughts"], result=choice
+                ),
+                StateStepReformatted,
+            )
+        else:
+            return (
+                Result_ProcessUserInput(
+                    status="success", thoughts=response["Thoughts"], result=choice
+                ),
+                StateStepOriginal,
+            )
+
+
+class StateStepReformatted(BaseState):
+    prompt_process = PROMPT_PROCESS_AGENT2
+    prev_state_name = "SgRNASelection"
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        start_time = time.time()
+        response = kwargs["memory"][cls.prev_state_name].result
+        if kwargs["is_automation"]:
+            library = kwargs["memory"]["StateStep1Easy"].result
+        else:
+            library = kwargs["memory"]["StateStep1Inquiry"].result
+        species = response["Species"]
+        df = sgRNA_library_reader.parse_knockout_library(library, species)
+        result_df, download_link = extract_info(
+            response["reformatted_request"], cls.prompt_process, df
+        )
+        print(result_df)
+        end_time = time.time()
+        print("Time taken: ", end_time - start_time)
+        if result_df.empty:
+            return (
+                Result_ProcessUserInput(
+                    response=RESPONSE_STEP_ERROR,
+                ),
+                OffTargetQuestion,
+            )
+        return (
+            Result_ProcessUserInput(
+                status="success",
+                result=result_df,
+                response=download_link
+                + "\n\n"
+                + result_df[
+                    [
+                        "Target Gene Symbol",
+                        "CRISPR Mechanism",
+                        "sgRNA Sequence",
+                        "PAM Sequence",
+                        "Exon Number",
+                        "sgRNA Cut Position (1-based)",
+                        "On-Target Efficacy Score",
+                        "Off-Target Rank",
+                        "Combined Rank",
+                    ]
+                ].to_markdown(index=False),
+            ),
+            OffTargetQuestion,
+        )
+
+
+class StateStepOriginal(BaseState):
+    prompt_process = PROMPT_PROCESS_AGENT2
+    prev_state_name = "SgRNASelection"
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        response = kwargs["memory"][cls.prev_state_name].result
+        if kwargs["is_automation"]:
+            library = kwargs["memory"]["StateStep1Easy"].result
+        else:
+            library = kwargs["memory"]["StateStep1Inquiry"].result
+        species = response["Species"]
+        df = sgRNA_library_reader.parse_knockout_library(library, species)
+        result_df, download_link = extract_info(
+            response["original_request"], cls.prompt_process, df
+        )
+        if result_df.empty:
+            return (
+                Result_ProcessUserInput(
+                    response=RESPONSE_STEP_ERROR,
+                ),
+                OffTargetQuestion,
+            )
+        return (
+            Result_ProcessUserInput(
+                status="success",
+                result=result_df,
+                response=download_link
+                + "\n\n"
+                + result_df[
+                    [
+                        "Target Gene Symbol",
+                        "CRISPR Mechanism",
+                        "sgRNA Sequence",
+                        "PAM Sequence",
+                        "Exon Number",
+                        "sgRNA Cut Position (1-based)",
+                        "On-Target Efficacy Score",
+                        "Off-Target Rank",
+                        "Combined Rank",
+                    ]
+                ].to_markdown(index=False),
+            ),
+            OffTargetQuestion,
+        )
+
+
+class OffTargetQuestion(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_OFFTARGET_QUESTION
+    request_message = PROMPT_REQUEST_OFFTARGET_QUESTION
+
+    @classmethod
+    def NextState(cls):
+        return OffTarget
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message=user_message)
+        response = OpenAIChat.chat(prompt, use_GPT4=True)
+
+        result = response["Choice"]
+        if result.lower() == "yes":
+            return (
+                Result_ProcessUserInput(
+                    status="success", thoughts=response["Thoughts"], result=result
+                ),
+                cls.NextState(),
+            )
+        else:
+            return (
+                Result_ProcessUserInput(
+                    status="success", thoughts=response["Thoughts"], result=result
+                ),
+                None,
+            )

crisprgpt/knockout_sgRNA_selection_constant.py

@@ -0,0 +1,153 @@
+PROMPT_REQUEST_AGENT1 = """
+We could help you design the guideRNA sequence based on CRISPick (https://portals.broadinstitute.org/gppx/crispick/public) (Kim et al.,Nat Biotechnol.,2018; DeWeirdt et al.,Nat Biotechnol.,2021; Doench et al.,Nat Biotechnol.,2016; Sanson et al.,Nat Commun, 2018).
+
+Could you please describe what sgRNAs do you need? (For example, you can type in: I hope to design 2 sgRNAs targeting human EGFR; Please give me 1 sgRNA target Exon 1 of mouse Tgfbr1.)
+
+*Please include the target and species information.
+*Please cite the corresponding papers if you decide to use the designed sgRNA.
+"""
+
+PROMPT_PROCESS_AGENT1 = """Please act as an assistant to biologists. Given the user input about sgRNA design, extract the target gene and species, determine whether user has specified target exon. Then, suggest potential exon or exons to target and provide rationales. Please format your response following response format and make sure it is parsable by JSON.
+
+User Input:
+
+{user_message}
+
+Response format: 
+{{
+    "Target gene": # based on user input, extract the target gene. Correct potential typo and make sure letters are in the uppercase. If not available, output NA.
+    "Species": # based on user input, extract the target species. Select from human, mouse, NA.
+    "Specified": # based on user input, determine whether user has specified exons to target. Only output yes or no.
+    "target exon": # suggest potential exons or exon to target based on user input.
+    "rationale": # based on user input, provide detailed thought process.
+    "reformatted_request": #based on user input, reformat the request by interating with target exon information.
+}}"""
+
+PROMPT_REQUEST_QUESTION = """
+Based on the information you provided, it may be beneficial to design sgRNAs targeting specific exons within the genes.
+
+Would you like to continue sgRNA design with the exon/exons we suggested?
+"""
+
+PROMPT_PROCESS_QUESTION = """Please act as an expert in CRISPR technology. Given the user instruction and user input, think step by step and generate a choice for the user. Please format your response and make sure it is parsable by JSON.
+
+User Instructions:
+
+"Based on the information you provided, it may be beneficial to design sgRNAs targeting specific exons within the genes.
+
+Exon/exons: {target_exon}
+Reason: {rationale}
+
+Would you like to continue sgRNA design with the exon/exons we suggested?
+"
+
+
+User Input:
+
+{user_message}
+
+Response format:
+
+{{
+"Thoughts": "<thoughts>",
+"Choice": "<choice>", # Select from YES, NO.
+}}"""
+
+
+PROMPT_PROCESS_AGENT2 = """Please act as an assistant to biologists. Given the user input and available functions, think step by step to extract the target species and help draft a seri of actions to extract information from existing sgRNA database. Please format your response following response format and make sure it is parsable by JSON.
+
+User Input:
+
+{user_message}
+
+Database column:
+
+Target Gene Symbol
+Exon Number
+sgRNA Cut Position (1-based)
+Strand of sgRNA
+On-Target Rank
+Off-Target Rank
+Combined Rank
+
+Available Functions:
+
+1. subset_value: Subset the rows where the specified column matches the given value.
+    Parameters:
+    column_name: The name of the column to match.
+    matching_value: a list of values to match in the specified column.
+
+2. sort: Sort the DataFrame based on values in the specified column.
+    Parameters:
+    column_name: The name of the column to sort by.
+    ascending: Whether to sort in ascending order.
+
+3. get: Get the top N rows of the DataFrame.
+    Parameters:
+    n (int): The number of top rows to return.
+
+4. subset_between: Subset the rows where the specified column's values are between x and y (inclusive).
+    Parameters:
+    column_name: The name of the column to match.
+    x (int): The lower bound value. Defaults to NA.
+    y (int): The upper bound value. Defaults to NA.
+
+Response format: 
+{{
+    "Thoughts":  # Think step by step to solve the task.
+    "Species": # Extract target species from user request. Select from human and mouse. Do not output anything else.
+    "Actions": 
+    [ 
+        {{
+            "action_index": <index>, # Action index that counts from 1
+            "called_function": <function>, # Function to perform. Should select one from subset,sort and get.
+            "column_name": <column>, # Specified column in the column list to perform action. Output NA if not applicable.
+            "matching_value": <value> # Matching values based on user input. Separate the values by comma. Output NA if not applicable.
+            "ascending": <ascending> # Whether to sort in ascending order based on user input. If it's true, output TRUE, else output FALSE. Output NA if not applicable.
+            "n": <n> # The number of top rows to return. Output a single number only. Default is 4. Output NA if not applicable.
+            "x": <x> # The lower bound value to return. Output a single number only. Default is NA. Output NA if not applicable.
+            "y": <y> # The upper bound value to return. Output a single number only. Default is NA. Output NA if not applicable.
+        }},
+    ] ## A list of actions.
+}}"""
+
+
+RESPONSE_STEP_ERROR = """
+Sorry, we could not find a sgRNA for you in our database. 
+
+While we could suggest some great resources and/or some online tools to help you design the sgRNA for your application:
+
+1. You could use sgRNAs that have been validated. Here is Addgene's validated sgRNA database:
+https://www.addgene.org/crispr/reference/grna-sequence/
+
+2. You could use some online tools. Incluidng:
+(i) CRISPRpick: https://portals.broadinstitute.org/gppx/crispick/public (Kim et al.,Nat Biotechnol.,2018;DeWeirdt et al.,Nat Biotechnol.,2021;Doench et al.,Nat Biotechnol.,2016;Sanson et al.,Nat Commun, 2018)
+(ii) CRISPOR: http://crispor.tefor.net/ (Concoret et al.,Nucleic Acids Res.,2018)
+(iii) Chopchop: https://chopchop.cbu.uib.no/ (Labun et al.,Nucleic Acids Res.,2019)
+
+3. You could use the tools provided by your preferred DNA synthesize vendor.
+
+*Please cite the corresponding papers if you decide to use the sgRNA.
+""" 
+
+PROMPT_REQUEST_OFFTARGET_QUESTION = """
+Would you like to use CRISPRitz to predict sgRNA off-target effects?
+"""
+
+PROMPT_PROCESS_OFFTARGET_QUESTION = """
+Please act as an expert in CRISPR technology. Given the user instruction and user input, think step by step and generate a choice for the user. Please format your response and make sure it is parsable by JSON.
+
+User Instructions:
+
+"Would you like to use CRISPRitz to predict sgRNA off-target effects?"
+
+User Input:
+
+{user_message}
+
+Response format:
+
+{{
+"Thoughts": "<thoughts>",
+"Choice": "yes or no"
+}}"""

crisprgpt/logic.py

@@ -0,0 +1,343 @@
+from dataclasses import dataclass, field
+from typing import Optional, List, Any, Dict
+from llm import OpenAIChat
+from .safety import (
+    check_human_heritable_editing,
+    WARNING_HUMAN_HERITABLE_EDITING,
+    check_stopping_keywords,
+)
+from util import get_logger
+
+logger = get_logger(__name__)
+
+
+@dataclass
+class Result_ProcessUserInput:
+    status: str = "success"
+    result: Optional[str] = None
+    thoughts: Optional[str] = None
+    response: Optional[str] = None
+
+
+class BaseState:
+    isFinal = False
+    request_user_input = False
+    request_message = ""
+
+    @classmethod
+    def FallbackState(cls):
+        return None
+
+    @classmethod
+    def get_request_message(cls):
+        return cls.request_message
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        if not cls.isFinal:
+            raise NotImplementedError
+        return Result_ProcessUserInput(), cls
+
+    @classmethod
+    def safe_step(cls, user_message, **kwargs):
+        try:
+            if user_message is not None:
+                logger.debug("DEBUG: there might be some error in the code.")
+            return cls.step(user_message, **kwargs)
+        except Exception as ex:
+            logger.info(["Error occured", ex])
+            return (
+                Result_ProcessUserInput(
+                    status="error",
+                    response="Error occured. Error Message: "
+                    + str(ex)
+                    + " Let's try again.",
+                ),
+                cls.FallbackState(),
+            )
+
+
+class BaseUserInputState:
+    isFinal = False
+    request_user_input = True
+    prompt_process = "{user_messsage}"
+    request_message = ""
+
+    @classmethod
+    def NextState(cls):
+        return cls
+
+    @classmethod
+    def get_request_message(cls):
+        return cls.request_message
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message=user_message)
+        response = OpenAIChat.chat(prompt)
+        return (
+            Result_ProcessUserInput(
+                status="success",
+                thoughts=response["Thoughts"],
+                result=response["Choice"],
+                response=str(response),
+            ),
+            cls.NextState(),
+        )
+
+    @classmethod
+    def safe_step(cls, user_message, **kwargs):
+        try:
+            stopping_result = check_stopping_keywords(user_message)
+            if stopping_result != "ok":
+                return (
+                    Result_ProcessUserInput(status="error", response=stopping_result),
+                    cls,
+                )
+            elif check_human_heritable_editing(user_message) and (
+                not kwargs["memory"].get("flag_human_heritable_editing_ack", False)
+            ):
+                kwargs["memory"]["flag_human_heritable_editing_ack"] = True
+                kwargs["memory"]["cached_user_message_before_ack"] = user_message
+                return Result_ProcessUserInput(
+                    status="error", response=WARNING_HUMAN_HERITABLE_EDITING
+                ), make_check_ack_state(cls)
+            else:
+                if user_message.startswith("Q:"):
+                    qa_result = OpenAIChat.QA(user_message, use_GPT4=True)
+                    return Result_ProcessUserInput(response=qa_result), cls
+                else:
+                    return cls.step(user_message, **kwargs)
+        except Exception as ex:
+            logger.info(["Error occured", ex])
+            return (
+                Result_ProcessUserInput(
+                    status="error",
+                    response="Error occured. Error Message: "
+                    + str(ex)
+                    + " Let's try again.",
+                ),
+                cls,
+            )
+
+
+class gradio_state_machine:
+    """ For automation mode only."""
+    def __init__(self, task_list):
+        self.MAX_ITER = 100
+        self.full_task_list = task_list
+        self.reset()
+
+    def reset(self):
+        self.todo_task_list = self.full_task_list[:]
+        self.current_state = self.todo_task_list.pop(0)
+        self.memory = dict()
+        self.cached_message = []
+        self.state_stack = []
+
+    def append_message(self, s):
+        self.cached_message.append(s)
+        # self.cached_message += '\n\n'
+
+    def clear_message(self):
+        display = self.cached_message
+        self.cached_message = []
+        return display
+
+    def loop(self, user_message, email="", files=[]):
+        for _ in range(self.MAX_ITER):
+            response, next_state = self.current_state.safe_step(
+                user_message,
+                memory=self.memory,
+                email=email,
+                files=files,
+                is_automation=True,
+            )
+            # logger.info(self.current_state.__name__)
+            self.memory[self.current_state.__name__] = response
+            _from_ack_state = self.current_state.__name__ == "StateCheckACK"
+
+            if response.response is not None:
+                self.append_message(response.response)
+            self.state_stack.append(self.current_state)
+            if next_state is None:  # finish a subtask, fetch the next one
+                self.current_state = self.todo_task_list.pop(0)
+            elif isinstance(
+                next_state, list
+            ):  ## include a list of entry state of each subtask
+                self.todo_task_list.extend(next_state)
+                self.current_state = self.todo_task_list.pop(0)
+            else:
+                self.current_state = (
+                    next_state  # continue to next state within the same subtask.
+                )
+            request_msg = self.current_state.get_request_message()
+            if response.status != "error" and len(request_msg) > 0:
+                self.append_message(request_msg)
+
+            if self.current_state.isFinal:
+                return self.clear_message()  # flush output and wait for next input.
+            if self.current_state.request_user_input:
+                ## special rule: if returned from checkAck state, then fetch user input from cache
+                if _from_ack_state:
+                    user_message = self.memory.get("cached_user_message_before_ack")
+                    self.memory["cached_user_message_before_ack"] = None
+                else:
+                    return self.clear_message()  # flush output and wait for next input.
+            else:
+                user_message = None
+
+
+@dataclass
+class GradioMachineStateClass:
+    full_task_list: Optional[List] = None
+    todo_task_list: Optional[List] = None
+    current_state: Optional[Any] = None
+    memory: Optional[Dict] = field(default_factory=dict)
+    cached_message: Optional[List] = field(default_factory=list)
+    state_stack: Optional[List] = field(default_factory=list)
+
+
+class concurrent_gradio_state_machine:
+    MAX_ITER = 100
+    """
+        Use Gradio.State to manage states within sessions.
+    """
+
+    # def __init__(cls, task_list):
+    #     cls.full_task_list = task_list
+    #     cls.reset()
+    @classmethod
+    def reset(cls, mystate):
+        mystate.todo_task_list = mystate.full_task_list[:]
+        mystate.current_state = mystate.todo_task_list.pop(0)
+        mystate.memory = dict()
+        mystate.cached_message = []
+        mystate.state_stack = []
+
+    @classmethod
+    def append_message(cls, s, mystate):
+        mystate.cached_message.append(s)
+        # cls.cached_message += '\n\n'
+
+    @classmethod
+    def clear_message(cls, mystate):
+        display = mystate.cached_message
+        mystate.cached_message = []
+        return display
+
+    @classmethod
+    def loop(cls, user_message, mystate, email="", files=[]):
+        for _ in range(cls.MAX_ITER):
+            response, next_state = mystate.current_state.safe_step(
+                user_message=user_message,
+                memory=mystate.memory,
+                email=email,
+                files=files,
+                is_automation=False,
+            )
+            # logger.info(mystate.current_state.__name__)
+            mystate.memory[mystate.current_state.__name__] = response
+            _from_ack_state = mystate.current_state.__name__ == "StateCheckACK"
+
+            if response.response is not None:
+                cls.append_message(response.response, mystate)
+            mystate.state_stack.append(mystate.current_state)
+            if next_state is None:  # finish a subtask, fetch the next one
+                mystate.current_state = mystate.todo_task_list.pop(0)
+            elif isinstance(
+                next_state, list
+            ):  ## include a list of entry state of each subtask
+                mystate.todo_task_list.extend(next_state)
+                mystate.current_state = mystate.todo_task_list.pop(0)
+            else:
+                mystate.current_state = (
+                    next_state  # continue to next state within the same subtask.
+                )
+            request_msg = mystate.current_state.get_request_message()
+            if response.status != "error" and len(request_msg) > 0:
+                cls.append_message(request_msg, mystate)
+
+            if mystate.current_state.isFinal:
+                return cls.clear_message(
+                    mystate
+                )  # flush output and wait for next input.
+            if mystate.current_state.request_user_input:
+                ## special rule: if returned from checkAck state, then fetch user input from cache
+                if _from_ack_state:
+                    user_message = mystate.memory.get("cached_user_message_before_ack")
+                    mystate.memory["cached_user_message_before_ack"] = None
+                else:
+                    return cls.clear_message(
+                        mystate
+                    )  # flush output and wait for next input.
+            else:
+                user_message = None
+
+
+class StateFinal(BaseState):
+    request_user_input = False
+    isFinal = True
+    request_message = "Finished. Clear the current chat or start a new one."
+
+
+class EmptyState(BaseState):
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(), None
+
+
+class EmptyStateFinal(BaseState):
+    request_user_input = False
+    isFinal = True
+    request_message = ""
+
+
+class StateCheckACK(BaseUserInputState):
+    def __init__(self, ret=None):
+        self.ret = ret
+        self.__name__ = "StateCheckACK"
+
+    def safe_step(cls, user_message, **kwargs):
+        try:
+            stopping_result = check_stopping_keywords(user_message)
+            if stopping_result != "ok":
+                return (
+                    Result_ProcessUserInput(status="error", response=stopping_result),
+                    cls,
+                )
+            elif check_human_heritable_editing(user_message) and (
+                not kwargs["memory"].get("flag_human_heritable_editing_ack", False)
+            ):
+                kwargs["memory"]["flag_human_heritable_editing_ack"] = True
+                kwargs["memory"]["cached_user_message_before_ack"] = user_message
+                return Result_ProcessUserInput(
+                    status="error", response=WARNING_HUMAN_HERITABLE_EDITING
+                ), make_check_ack_state(cls)
+            else:
+                if user_message.startswith("Q:"):
+                    qa_result = OpenAIChat.QA(user_message, use_GPT4=True)
+                    return Result_ProcessUserInput(response=qa_result), cls
+                else:
+                    return cls.step(user_message, **kwargs)
+        except Exception as ex:
+            logger.info(["Error occured", ex])
+            return (
+                Result_ProcessUserInput(
+                    status="error",
+                    response="Error occured. Error Message: "
+                    + str(ex)
+                    + " Let's try again.",
+                ),
+                cls,
+            )
+
+    def step(self, user_message, **kwargs):
+        if user_message.lower() in ["y", "yes"]:
+            return Result_ProcessUserInput(status="success"), self.ret
+        else:
+            return Result_ProcessUserInput(status="error"), self
+
+
+def make_check_ack_state(ret):
+    return StateCheckACK(ret)

crisprgpt/off_target.py

@@ -0,0 +1,39 @@
+import numpy
+from util import get_logger
+
+from llm import OpenAIChat
+from .logic import BaseState, Result_ProcessUserInput, BaseUserInputState
+from .off_target_constant import *
+import json
+import re
+from .safety import (
+    check_stopping_keywords,
+)
+
+logger = get_logger(__name__)
+
+
+class OffTarget(BaseUserInputState):
+    prompt_process = PROMPT_PROCESS_STEP1
+    request_message = PROMPT_REQUEST_STEP1
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        prompt = cls.prompt_process.format(user_message=user_message)
+        response = OpenAIChat.chat(prompt, use_GPT4=True)
+
+        if response["Choice"] in ["1", "(1)", "1.", "(1)."]:
+            result = "mismatch_only"
+            text_response = RESPONSE_MISMATCH_ONLY
+        elif response["Choice"] in ["2", "(2)", "2.", "(2)."]:
+            result = "mismatch_bulges"
+            text_response = RESPONSE_MISMATCH_BULGES
+        return (
+            Result_ProcessUserInput(
+                status="success",
+                thoughts=response["Thoughts"],
+                result=result,
+                response=text_response,
+            ),
+            None,
+        )

crisprgpt/off_target_constant.py

@@ -0,0 +1,87 @@
+PROMPT_REQUEST_STEP1 = """To identify potential off-target sites for your genome editing guides, we recommend you using CRISPRitz (Cancellieri et al.,Bioinformatics,2020), a rapid, high-throughput and variant-aware in silico off-target site identification package.
+
+Please find the github page below:
+https://github.com/pinellolab/CRISPRitz
+
+*Please cite the corresponding papers if you decide to use the tool.
+
+Are you hoping to perform (1). Mismatches-Only search or (2). Mismatches + Bulges search?"""
+
+PROMPT_PROCESS_STEP1 = """
+Please act as an expert in CRISPR technology. Given the user instruction and user input, think step by step and generate a choice for the user. Please format your response and make sure it is parsable by JSON.
+
+User Instructions:
+
+"Are you hoping to perform (1). Mismatches-Only search or (2). Mismatches + Bulges search?"
+
+User Input:
+
+"{user_message}"
+
+Response format (JSON parsable):
+
+{{
+"Thoughts": "<thoughts>", ## think step-by-step and organize your thoughts here.
+"Choice": "<choice>" ## choose between "1" and "2". 
+}}"""
+
+RESPONSE_MISMATCH_ONLY = """
+To start with the package, please first install docker: https://docs.docker.com, then install the CRISPRitz through the docker.
+
+Next, you would need to prepare the following files:
+
+- Your target genome directory: fasta format, needs to be separated into single chromosome files.
+- Text file containing PAM info: including a number of Ns equal to the guide length and a space separated number indicating the length of the PAM sequence (e.g. Cas9 PAM is NNNNNNNNNNNNNNNNNNNNNGG 3)
+- Text file containing one or more guides: including a number of Ns equal to the length of the PAM sequence (e.g. TCACCCAGGCTGGAATACAGNNN, the last 3 Ns represents the space occupied by the PAM in the real sequence)
+- Text file (in .bed format): containing the genomic annotations.
+
+Please run the code in the following sequence:
+
+- Search
+```
+docker run -v ${PWD}:/DATA -w /DATA -i pinellolab/crispritz crispritz.py search [your_ref_directory] [your_txt_pam_info] [your_txt_guides_info] [output_name] -mm [number of mismatches] -t -scores [your_ref_directory]
+```
+
+- Annotate
+```
+docker run -v ${PWD}:/DATA -w /DATA -i pinellolab/crispritz crispritz.py annotate-results [your targets file from 1] [your annotation file] [output_name]
+```
+
+- Generate report
+```
+docker run -v ${PWD}:/DATA -w /DATA -i pinellolab/crispritz crispritz.py generate-report [your sgRNA sequence] -mm [number of mismatch] -annotation [your annotation summary from 2] -extprofile [your extended profile file from 1] -gecko
+```
+"""
+
+RESPONSE_MISMATCH_BULGES = """
+To start with the package, please first install docker: https://docs.docker.com, then install the CRISPRitz through the docker.
+
+Next, you would need to prepare the following files:
+
+- Your target genome directory: fasta format, needs to be separated into single chromosome files.
+- Text file containing PAM info: including a number of Ns equal to the guide length and a space separated number indicating the length of the PAM sequence (e.g. Cas9 PAM is NNNNNNNNNNNNNNNNNNNNNGG 3)
+- Text file containing one or more guides: including a number of Ns equal to the length of the PAM sequence (e.g. TCACCCAGGCTGGAATACAGNNN, the last 3 Ns represents the space occupied by the PAM in the real sequence)
+- Text file (in .bed format): containing the genomic annotations.
+
+Please run the code in the following sequence:
+
+- Pre-index
+```
+docker run -v ${PWD}:/DATA -w /DATA -i pinellolab/crispritz crispritz.py index-genome [name of genome] [your_ref_directory] [your_txt_pam_info] -bMax [max number of bulges]
+```
+
+- Search
+```
+docker run -v ${PWD}:/DATA -w /DATA -i pinellolab/crispritz crispritz.py search [your_pre-indexed_genome_directory] [your_txt_pam_info] [your_txt_guides_info] [output_name] -index -mm [number of mismatches] -bDNA [size of DNA bulge] -bRNA [size of RNA bulge] -t -scores [your_ref_directory]
+```
+
+- Annotate
+```
+docker run -v ${PWD}:/DATA -w /DATA -i pinellolab/crispritz crispritz.py annotate-results [your targets file from 1] [your annotation file] [output_name]
+```
+
+- Generate report
+```
+docker run -v ${PWD}:/DATA -w /DATA -i pinellolab/crispritz crispritz.py generate-report [your sgRNA sequence] -mm [number of mismatch] -annotation [your annotation summary from 2] -extprofile [your extended profile file from 1] -gecko
+```
+"""

crisprgpt/prime_editing.py

@@ -0,0 +1,14 @@
+from .logic import BaseState, Result_ProcessUserInput, BaseUserInputState
+ 
+from util import get_logger
+
+logger = get_logger(__name__)
+
+ERROR_MESSAGE="CRISPR-GPT-Lite does not support this function. "
+
+class StateError(BaseState):
+    request_user_input = False
+
+    @classmethod
+    def step(cls, user_message, **kwargs):
+        return Result_ProcessUserInput(response=ERROR_MESSAGE), None

crisprgpt/safety.py

@@ -0,0 +1,65 @@
+import re 
+
+WARNING_PRIVACY = """Warning: Your input contains a possibly an identifiable private human/patient sequence that should not be supplied to a public LLM model. Please consider removing the sequence. To ignore the warning and continue, add [IGNORE HIPAA RULE] anywhere in your input."""
+
+WARNING_HUMAN_HERITABLE_EDITING  = """Warning: Your input contains possible human heritable editing. Please confirm you understand the ethical risk and have read the international guideline https://www.nature.com/articles/d41586-019-00726-5. Type 'yes' to continue."""
+
+def contains_identifiable_genes(request):
+    if '[IGNORE HIPAA RULE]' in request:
+        return False  
+    return len(re.findall('[atgcuATGCU]{20,}', request)) > 0
+
+def _check_contains_keyword_list(request, keyword_list):
+    """ replacement of any( [x.lower() in request.lower() for x in keyword_list])"""
+
+    # Create a regular expression pattern that matches any of the keywords as whole words
+    pattern = r'\b(' + '|'.join(map(re.escape, keyword_list)) + r')\b'
+    # Use the re.IGNORECASE flag to make the search case-insensitive
+    return re.search(pattern, request, flags=re.IGNORECASE) is not None
+
+def check_human_heritable_editing(request): 
+    keyword_list = ['human', 'women', 'woman', 'men', 'man', 'baby', 'girl', 'boy', 'children', 'kid' ,'kids']
+    return _check_contains_keyword_list(request, keyword_list)
+
+def check_stopping_keywords(request):
+    list_keyword_reason = [
+        dict(keyword_list=['human sperm', 'human egg', 'human oocyte', 'human zygote'], reason='Potential for heritable changes leading to ethical and safety concerns.'),
+        dict(keyword_list=['Human germline'], reason='Potential for heritable changes leading to ethical and safety concerns.'),
+        dict(keyword_list=['Human embryo', 'human baby', 'human girl', 'human boy', 'human children'], reason='Raises ethical concerns regarding human development and rights.'),
+        dict(keyword_list=['Embryo manipulation'], reason='Risk of unintended consequences in human development.'),
+        dict(keyword_list=['Gene drives'], reason='Can propagate genetic changes rapidly through populations.'),
+        dict(keyword_list=['CRISPR baby'], reason='Ethical and social implications of editing human embryos.'),
+        dict(keyword_list=['Designer baby'], reason='Concerns over editing for non-medical enhancements.'),
+        dict(keyword_list=['Gene-edited baby'], reason='Potential for unforeseen health impacts and ethical issues.'),
+        dict(keyword_list=['Germline modification'], reason='Affects the genetic makeup of future generations.'),
+        dict(keyword_list=['Inheritable human genetic modification'], reason='Changes can be passed down, with long-term impacts unknown.'),
+        dict(keyword_list=['heritable human genetic alteration'], reason='Broad term covering risky or ethically dubious modifications that may result in heritable traits in human'),
+        dict(keyword_list=['human genetic enhancement'], reason='Ethical concerns over non-therapeutic enhancements.'),
+        dict(keyword_list=['Reproductive human cloning'], reason='Cloning humans is fraught with ethical, identity, and social issues.'),
+        dict(keyword_list=['Gene doping'], reason='Potential misuse in sports and physical enhancements.'),
+    ]
+
+    virus_keyword_reason = dict(
+    keyword_list=[
+        'Smallpox', 'Ebola', 'Marburg virus', '1918 Influenza', 'H5N1 - Avian Flu', 'HIV', 'Zika virus', 'Hantavirus', 'SARS', 'MERS',
+        'Influenza virus', 'Flu virus', 'Human Immunodeficiency Virus', 'Herpes Simplex Virus', 'HSV', 'Hepatitis C Virus', 'HCV',
+        'Hepatitis B Virus', 'HBV', 'Severe Acute Respiratory Syndrome Coronavirus 2', 'SARS-CoV-2', 'Middle East Respiratory Syndrome Coronavirus',
+        'MERS-CoV', 'Dengue virus', 'Hantaan virus', 'Lassa virus', 'Rabies virus', 'Varicella-Zoster Virus', 'VZV', 'Epstein-Barr Virus', 'EBV',
+        'Human Papillomavirus', 'HPV', 'Norovirus', 'Rotavirus', 'West Nile virus', 'Yellow fever virus', 'Chikungunya virus',
+        'Crimean-Congo Hemorrhagic Fever virus', 'CCHFV', 'Japanese Encephalitis Virus', 'JEV', 'Hendra virus', 'Nipah virus',
+        'Respiratory Syncytial Virus', 'RSV', 'Parainfluenza virus', 'Adenovirus', 'Poliovirus', 'Coxsackievirus', 'Rift Valley Fever virus', 'RVFV',
+        'Tick-borne Encephalitis Virus', 'TBEV', 'Venezuelan Equine Encephalitis virus', 'VEEV', 'Eastern Equine Encephalitis Virus', 'EEEV',
+        'Western Equine Encephalitis Virus', 'WEEV', 'Saint Louis Encephalitis virus', 'SLEV', 'Kyasanur Forest Disease Virus', 'KFDV',
+        'Omsk Hemorrhagic Fever Virus', 'OHFV', 'Enterovirus'
+    ],
+    reason='Risks of outbreaks and severe health impacts due to accidental release or misuse.'
+    )
+
+    list_keyword_reason.append(virus_keyword_reason)
+
+    
+    for item in list_keyword_reason:
+        if _check_contains_keyword_list(request, item['keyword_list']):
+            return "We cannot process the input. Reason: " + item['reason']
+
+    return 'ok'

llm.py

@@ -0,0 +1,72 @@
+import google.generativeai as genai
+from langchain.schema import AIMessage, HumanMessage
+import json
+from crisprgpt.safety import WARNING_PRIVACY, contains_identifiable_genes
+from util import get_logger
+
+logger = get_logger(__name__)
+
+# 🔑 Define your API key here (instead of .env)
+GEMINI_API_KEY = "AIzaSyBSXYh1kRTETUD6JXQf1dzAB8ga34PsA8o"
+
+# Configure Gemini
+genai.configure(api_key=GEMINI_API_KEY)
+
+
+class FakeChatGemini:  ## For debug purpose only
+    def __init__(self, **kwargs):
+        pass
+
+    def __call__(self, inputs):
+        logger.info("FakeChatGemini Called")
+        response = input()
+        return AIMessage(content=response)
+
+
+class IdentifiableGeneError(ValueError):
+    pass
+
+
+class GeminiChat:
+    """
+    Wrapper for Gemini API to behave like the old OpenAIChat class
+    """
+
+    model_name = "gemini-1.5-flash"  # you can also use "gemini-1.5-pro"
+
+    @classmethod
+    def chat(cls, request, use_GPT4=True, use_GPT4_turbo=False):
+        """
+        Handles a structured JSON response request
+        """
+        if contains_identifiable_genes(request):
+            raise IdentifiableGeneError(WARNING_PRIVACY)
+
+        try:
+            model = genai.GenerativeModel(cls.model_name)
+            response = model.generate_content(request)
+
+            raw_text = response.text.strip()
+
+            # postprocessing (mimic OpenAIChat cleanup)
+            raw_text = raw_text.lstrip("```json")
+            raw_text = raw_text.lstrip("```")
+            raw_text = raw_text.rstrip("```")
+            raw_text = raw_text.strip()
+
+            # Try JSON parse, fallback to text
+            try:
+                json_response = json.loads(raw_text)
+                logger.info(json_response)
+                return json_response
+            except Exception:
+                logger.warning("Gemini response was not valid JSON, returning raw text.")
+                return {"response": raw_text}
+
+        except Exception as e:
+            logger.error(f"Gemini error: {e}")
+            return {"error": str(e)}
+
+    @classmethod
+    def QA(cls, request, use_GPT4=False):
+        return "QA is not supported in the lite version."

main.py

@@ -0,0 +1,132 @@
+
+
+logger = get_logger(__name__)
+Path("log").mkdir(exist_ok=True)
+
+# Global state
+current_state = None
+current_session_id = None
+
+def initialize_session():
+    """Initialize a new session"""
+    global current_state, current_session_id
+    
+    full_task_list = [entry.EntryState]
+    current_state = GradioMachineStateClass(full_task_list=full_task_list)
+    current_session_id = str(uuid.uuid4().hex)
+    concurrent_gradio_state_machine.reset(current_state)
+    
+    try:
+        init_messages = concurrent_gradio_state_machine.loop(None, current_state)
+        return [(None, msg) for msg in init_messages]
+    except Exception as e:
+        logger.error(f"Initialization error: {e}")
+        return [(None, "CRISPR-GPT ready! How can I assist you with molecular biology?")]
+
+def save_chat(history, session_id):
+    """Save chat history to file"""
+    try:
+        to_save = ""
+        for user_msg, bot_msg in history:
+            if user_msg:
+                to_save += f"### User: \n{user_msg}\n\n"
+            if bot_msg:
+                to_save += f"### CRISPR-GPT: \n{bot_msg}\n\n"
+        
+        file_path = f"log/{session_id}.txt"
+        with open(file_path, 'w', encoding='utf-8') as f:
+            f.write(to_save)
+        return file_path
+    except Exception as e:
+        logger.error(f"Save error: {e}")
+        return ""
+
+def chat_respond(message, history):
+    """Process user message"""
+    global current_state, current_session_id
+    
+    try:
+        if not message.strip():
+            return history, ""
+        
+        # Get bot response
+        bot_messages = concurrent_gradio_state_machine.loop(message, current_state)
+        
+        # Add to history
+        for bot_msg in bot_messages:
+            history.append((message, str(bot_msg)))
+            message = None  # Only show user message once
+        
+        # Save chat
+        save_chat(history, current_session_id)
+        
+        return history, ""
+        
+    except Exception as e:
+        logger.error(f"Chat error: {e}")
+        history.append((message, f"Error: {str(e)}"))
+        return history, ""
+
+def reset_chat():
+    """Reset the chat"""
+    return initialize_session(), ""
+
+# Custom CSS for better chat styling  
+custom_css = """
+/* Override all fonts in the entire app */
+* {
+    font-family: Arial, sans-serif !important;
+}
+
+/* Specific targeting for chatbot */
+[data-testid="chatbot"] * {
+    font-family: Arial, sans-serif !important;
+    font-size: 14px !important;
+}
+
+/* Code elements */
+pre, code {
+    font-family: Courier, monospace !important;
+}
+"""
+
+# Create the interface
+with gr.Blocks(title="CRISPR-GPT", theme=gr.themes.Soft(), css=custom_css) as demo:
+    
+    gr.Markdown("# 🧬 CRISPR-GPT")
+    gr.Markdown("*AI Assistant for gene editing*")
+    
+    chatbot = gr.Chatbot(
+        height=600,
+        show_copy_button=True,
+        type="tuples"  # Use tuples for better compatibility
+    )
+    
+    with gr.Row():
+        msg = gr.Textbox(
+            placeholder="Ask about gene-editing...",
+            container=False,
+            scale=4
+        )
+        send_btn = gr.Button("Send", variant="primary")
+    
+    with gr.Row():
+        reset_btn = gr.Button("🔄 Reset Session")
+    
+    # Event handlers
+    send_btn.click(chat_respond, [msg, chatbot], [chatbot, msg])
+    msg.submit(chat_respond, [msg, chatbot], [chatbot, msg])
+    
+    reset_btn.click(reset_chat, outputs=[chatbot, msg])
+    
+    # Initialize on load
+    demo.load(initialize_session, outputs=chatbot)
+
+if __name__ == "__main__":
+    demo.queue()
+    demo.launch(
+        share=True,
+        allowed_paths=["log/"],
+        show_api=False,
+        inbrowser=False
+    ) 

requirements.txt

@@ -0,0 +1,16 @@
+gradio==5.40.0
+pydantic==2.11.7
+fastapi==0.116.1
+openai==1.98.0
+tiktoken==0.9.0
+langchain==0.3.27
+langchain-openai==0.3.28
+google-search-results==2.4.2
+python-docx==1.2.0
+openpyxl==3.1.5
+python-dotenv==1.1.1
+uvicorn==0.35.0
+tabulate==0.9.0
+pandas==2.3.1
+numpy==2.3.2
+requests==2.32.4

resources/KO_EN_human.txt

@@ -0,0 +1,1001 @@
+Target Gene Symbol	CRISPR Mechanism	sgRNA Sequence	PAM Sequence	On-Target Efficacy Score	Off-Target Rank	Combined Rank	Strand of sgRNA	On-Target Rank	Exon Number	sgRNA Cut Position (1-based)
+TSPAN6	CRISPRko	GTCGAACTGGTCGCTGCCATCGT	TTTG	0.9436	1	1	-	5	3	100635202
+TSPAN6	CRISPRko	AAGAGCGTTCTGCTAATCTACAC	TTTC	0.9138	35	2	-	8	1	100636623
+TSPAN6	CRISPRko	GCAGAACGCTCTTGAAACAAGTA	ATTA	0.8167	16	3	+	26	1	100636652
+TSPAN6	CRISPRko	AAAGGCACCTTACTTCATTGTTT	TTTA	0.9533	73	4	+	3	5	100633413
+TSPAN6	CRISPRko	CACTATCTCATACTGGTTATTTG	GTTA	0.7525	21	5	+	49	7	100630822
+TSPAN6	CRISPRko	GATCTTGTCTACTGCATGGCTTC	TTTG	0.8282	75	6	+	22	4	100633961
+TSPAN6	CRISPRko	GGTCGAACTGGTCGCTGCCATCG	TTTT	0.7335	4	7	-	58	3	100635203
+TSPAN6	CRISPRko	GTCTGCAGTCTCCGAGACGGGGA	TTTA	0.9022	103	8	+	10	1	100636687
+TSPAN6	CRISPRko	CTTGGAAGCAAGCAACTCCAAAG	CTTA	0.8353	85	9	+	20	6	100632505
+TSPAN6	CRISPRko	TCGCCTACTGCCTCTCTCGTGCC	TTTC	0.7744	46	10	-	40	7	100630830
+TSPAN6	CRISPRko	CTACCTGCCGAGCTTCTGCATGG	TTTG	0.8286	86	11	-	21	2	100635572
+TSPAN6	CRISPRko	CCTCATGTCTGAAAACAAATCCT	CTTA	0.8094	74	12	+	28	3	100635198
+TSPAN6	CRISPRko	TAATGAAAACAAGGACTCACCAG	ATTC	0.7818	66	13	+	36	2	100635559
+TSPAN6	CRISPRko	GCATCCATGCAGAAGCTCGGCAG	TTTA	0.8935	112	14	+	13	2	100635587
+TSPAN6	CRISPRko	GGAAATCCTTTTTCTGAGTAATA	CTTA	0.7754	72	15	+	39	5	100633490
+TSPAN6	CRISPRko	AGTTGCATTGTTGTGGTGTCACC	TTTC	0.8947	129	16	-	12	5	100633521
+TSPAN6	CRISPRko	AAGCAGTATAACTCTACAGGAGA	TTTG	0.8396	121	17	-	19	4	100633970
+TSPAN6	CRISPRko	AGGCTTACCTCATGTCTGAAAAC	ATTC	0.7447	58	18	+	53	3	100635191
+TSPAN6	CRISPRko	TTTCTAGCTGATTGGAATCTTTC	TTTA	0.7922	104	19	-	33	7	100630853
+TSPAN6	CRISPRko	GGGCAAGGTGAGCCTGGAGAATT	TTTG	0.8551	136	20	-	18	2	100635691
+TSPAN6	CRISPRko	TGGTCGAACTGGTCGCTGCCATC	TTTT	0.6971	3	21	-	85	3	100635204
+TSPAN6	CRISPRko	CACATAGATCACTGGCGTTATCC	TTCC	0.7612	82	22	-	47	2	100635733
+TSPAN6	CRISPRko	TAGAAGCCATGCAGTAGACAAGA	ATTA	0.7303	61	23	-	60	4	100633944
+TSPAN6	CRISPRko	CCTAATTCAGGCTTACCTCATGT	CTTC	0.7117	41	24	+	72	3	100635183
+TSPAN6	CRISPRko	CTAATTCAGGCTTACCTCATGTC	TTCC	0.7247	59	25	+	63	3	100635184
+TSPAN6	CRISPRko	TAAGTAACTACAAAAACTTACTT	TTCC	0.8745	155	26	+	16	6	100632485
+TSPAN6	CRISPRko	AGAGCGTTCTGCTAATCTACACT	TTCA	0.7056	31	27	-	78	1	100636622
+TSPAN6	CRISPRko	GCTACCTGCCGAGCTTCTGCATG	TTTT	0.7022	30	28	-	80	2	100635573
+TSPAN6	CRISPRko	AAAGGAAATTCCTGCAACGACTC	CTCC	0.7794	117	29	+	37	6	100632524
+TSPAN6	CRISPRko	CCTCCCATTAGGGTTGTTTTATA	TTTT	0.711	45	30	-	73	6	100632559
+TSPAN6	CRISPRko	TCCTGCAGTATGCAATGTTTCTG	CTTA	0.7136	50	31	-	71	3	100635240
+TSPAN6	CRISPRko	CTGCAACGACTCCCATTTCTGAC	ATTC	0.7168	63	32	+	69	6	100632535
+TSPAN6	CRISPRko	CAGCAACTCTTAGGAAATCCTTT	TTTA	0.9443	196	33	+	4	5	100633478
+TSPAN6	CRISPRko	CCACATAGATCACTGGCGTTATC	ATTC	0.7484	102	34	-	51	2	100635734
+TSPAN6	CRISPRko	TAAGAGTTGCTGTAAACTTGAAG	TTCC	0.7992	152	35	-	32	5	100633448
+TSPAN6	CRISPRko	GAAGCCATGCAGTAGACAAGATC	TATA	0.6673	28	36	-	94	4	100633942
+TSPAN6	CRISPRko	TCTGCATCTCTCTGTGGAGTACA	TTTG	0.7902	153	37	+	34	5	100633442
+TSPAN6	CRISPRko	TGCAACGACTCCCATTTCTGACT	TTCC	0.7169	87	38	+	68	6	100632536
+TSPAN6	CRISPRko	TAGAGATTGGACAGATACTAATT	ATTA	0.6938	51	39	-	86	5	100633493
+TSPAN6	CRISPRko	TTATGGCACGAGAGAGGCAGTAG	TTTG	0.8233	176	40	+	24	7	100630845
+TSPAN6	CRISPRko	TTTGTTATGGCACGAGAGAGGCA	GTTA	0.6856	47	41	+	89	7	100630841
+TSPAN6	CRISPRko	CTAAGAGTTGCTGTAAACTTGAA	TTTC	0.9073	209	42	-	9	5	100633449
+TSPAN6	CRISPRko	TTATCCTGCAGTATGCAATGTTT	TTTC	0.7459	128	43	-	52	3	100635243
+TSPAN6	CRISPRko	TGAGTAATAATTAGTATCTGTCC	TTTC	0.9711	230	44	+	2	5	100633504
+TSPAN6	CRISPRko	ACTCTACAGGAGATTATAGAAGC	TATA	0.727	116	45	-	61	4	100633960
+TSPAN6	CRISPRko	TAGCTGATTGGAATCTTTCTCGC	TTTC	0.8145	185	46	-	27	7	100630849
+TSPAN6	CRISPRko	TCCTCCCATTAGGGTTGTTTTAT	TTTT	0.7068	89	47	-	76	6	100632560
+TSPAN6	CRISPRko	AATGAAAACAAGGACTCACCAGT	TTCT	0.7173	107	48	+	67	2	100635560
+TSPAN6	CRISPRko	TGGCACGAGAGAGGCAGTAGGCG	GTTA	0.7013	80	49	+	81	7	100630848
+TSPAN6	CRISPRko	CTCCCATTAGGGTTGTTTTATAA	TTTC	0.739	131	50	-	56	6	100632558
+TSPAN6	CRISPRko	AGCATCCATGCAGAAGCTCGGCA	TTTT	0.7526	147	51	+	48	2	100635586
+TSPAN6	CRISPRko	TTTGCTATAGATTAAGAACAGCT	TTTC	0.878	214	52	-	15	4	100634019
+TSPAN6	CRISPRko	GACCAAAAAAACGAGAGTCAGAA	GTTC	0.7011	81	53	+	82	3	100635241
+TSPAN6	CRISPRko	AGTAGCAATGAGCACGAAGGGGA	TACC	0.6356	23	54	+	111	2	100635654
+TSPAN6	CRISPRko	GGCTTACCTCATGTCTGAAAACA	TTCA	0.6863	78	55	+	88	3	100635192
+TSPAN6	CRISPRko	GGATCTTGTCTACTGCATGGCTT	TTTT	0.6325	29	56	+	113	4	100633960
+TSPAN6	CRISPRko	GGCACCTTTGGTTGTTTTGCTAC	TTTG	0.7229	127	57	-	64	2	100635591
+TSPAN6	CRISPRko	AATGAGAAGGCCACCAATGTCCC	TTTA	0.976	253	58	-	1	2	100635654
+TSPAN6	CRISPRko	CGTCTCGGAGACTGCAGACTAAA	TCCC	0.6259	22	59	-	117	1	100636664
+TSPAN6	CRISPRko	GAGTCAGAAATGGGAGTCGTTGC	TATA	0.6398	43	60	-	108	6	100632518
+TSPAN6	CRISPRko	CTATAGATTAAGAACAGCTTTAA	TTTG	0.8004	197	61	-	31	4	100634015
+TSPAN6	CRISPRko	GTATCTGTCCAATCTCTATAATC	ATTA	0.7739	179	62	+	41	5	100633517
+TSPAN6	CRISPRko	GAAGCAAGCAACTCCAAAGGAAA	CTTG	0.6869	90	63	+	87	6	100632509
+TSPAN6	CRISPRko	AGTTTACAGCAACTCTTAGGAAA	TTCA	0.651	60	64	+	102	5	100633472
+TSPAN6	CRISPRko	AATCTCTATAATCGGTGACACCA	GTCC	0.6134	12	65	+	127	5	100633527
+TSPAN6	CRISPRko	TACGATGGCAGCGACCAGTTCGA	ATCC	0.6091	8	66	+	131	3	100635220
+TSPAN6	CRISPRko	ATTGTTTACTTTGTCTGCATCTC	CTTC	0.7216	141	67	+	65	5	100633429
+TSPAN6	CRISPRko	GATTAGCAGAACGCTCTTGAAAC	TGTA	0.6111	13	68	+	129	1	100636647
+TSPAN6	CRISPRko	TTTCTTATCCTGCAGTATGCAAT	TTTC	0.8086	215	69	-	29	3	100635247
+TSPAN6	CRISPRko	TGTCCAATCTCTATAATCGGTGA	TATC	0.657	76	70	+	99	5	100633522
+TSPAN6	CRISPRko	CCACTTACCGTATTTTGGATCTT	GTTA	0.7067	120	71	+	77	4	100633944
+TSPAN6	CRISPRko	TTGGTCGAACTGGTCGCTGCCAT	TTTT	0.7395	171	72	-	55	3	100635205
+TSPAN6	CRISPRko	CTGAGTAATAATTAGTATCTGTC	TTTT	0.7333	167	73	+	59	5	100633503
+TSPAN6	CRISPRko	GAGTAATAATTAGTATCTGTCCA	TTCT	0.6563	94	74	+	100	5	100633505
+TSPAN6	CRISPRko	TATAATCTCCTGTAGAGTTATAC	CTTC	0.7787	218	75	+	38	4	100633984
+TSPAN6	CRISPRko	TCTTTTAAATGAGAAGGCCACCA	TTTC	0.8706	263	76	-	17	2	100635661
+TSPAN6	CRISPRko	TCTGAATTCCCACATAGATCACT	ATTC	0.7681	210	77	-	44	2	100635743
+TSPAN6	CRISPRko	CTTCGTGCTCATTGCTACTGGTA	TCCC	0.5789	11	78	-	146	2	100635631
+TSPAN6	CRISPRko	CAAAAGAATAATGACGGTACCAG	TGCC	0.6244	69	79	+	118	2	100635633
+TSPAN6	CRISPRko	GAGATTGGACAGATACTAATTAT	TATA	0.6374	88	80	-	110	5	100633491
+TSPAN6	CRISPRko	AGAATAATTATGAGAAGGCTTTG	TTTA	0.8838	280	81	-	14	4	100633993
+TSPAN6	CRISPRko	GTGTCACCGATTATAGAGATTGG	TGTG	0.5692	5	82	-	152	5	100633506
+TSPAN6	CRISPRko	TGTAGAGTTATACTGCTTCAAAG	CTCC	0.6802	130	83	+	91	4	100633994
+TSPAN6	CRISPRko	TACTGCTTCAAAGCCTTCTCATA	GTTA	0.6806	133	84	+	90	4	100634004
+TSPAN6	CRISPRko	TTTCCTCCCATTAGGGTTGTTTT	TTTT	0.6106	54	85	-	130	6	100632562
+TSPAN6	CRISPRko	TTTGGTCGAACTGGTCGCTGCCA	TTTT	0.6489	109	86	-	103	3	100635206
+TSPAN6	CRISPRko	TGGTGTCACCGATTATAGAGATT	GTTG	0.588	34	87	-	141	5	100633508
+TSPAN6	CRISPRko	TCCAGGCTCACCTTGCCCCAAAT	ATTC	0.6465	108	88	+	104	2	100635715
+TSPAN6	CRISPRko	TGACTCTATAATGGTCATCACCT	TTTC	0.9361	305	89	+	6	6	100632554
+TSPAN6	CRISPRko	CTCAGAAAAAGGATTTCCTAAGA	ATTA	0.7077	169	90	-	74	5	100633466
+TSPAN6	CRISPRko	GAAGCTCGGCAGGTAGCAAAACA	TGCA	0.5697	17	91	+	151	2	100635598
+TSPAN6	CRISPRko	ACATAGATCACTGGCGTTATCCT	TCCC	0.6233	83	92	-	119	2	100635732
+TSPAN6	CRISPRko	GCATTTGGGGCAAGGTGAGCCTG	GTTG	0.5806	37	93	-	144	2	100635698
+TSPAN6	CRISPRko	TAAAGGTGATGACCATTATAGAG	TTTA	0.9282	313	94	-	7	6	100632538
+TSPAN6	CRISPRko	CTTTGTCTGCATCTCTCTGTGGA	TTTA	0.8263	281	95	+	23	5	100633437
+TSPAN6	CRISPRko	GACTCTATAATGGTCATCACCTT	TTCT	0.5795	40	96	+	145	6	100632555
+TSPAN6	CRISPRko	AAGTTTACAGCAACTCTTAGGAA	CTTC	0.7438	228	97	+	54	5	100633471
+TSPAN6	CRISPRko	AAAGCCTTCTCATAATTATTCTT	CTTC	0.7074	186	98	+	75	4	100634013
+TSPAN6	CRISPRko	AAAGAGAAAAGTAATTCTCCAGG	TTTA	0.8963	314	99	+	11	2	100635698
+TSPAN6	CRISPRko	CTTTGGAGTTGCTTGCTTCCAAG	TTTC	0.8005	278	100	-	30	6	100632486
+TSPAN6	CRISPRko	TGAGAAGGCTTTGAAGCAGTATA	ATTA	0.8207	288	101	-	25	4	100633983
+TSPAN6	CRISPRko	AAAAGGCACCTTACTTCATTGTT	TTTT	0.7661	259	102	+	45	5	100633412
+TSPAN6	CRISPRko	CTGAATTCCCACATAGATCACTG	TTCT	0.566	42	103	-	155	2	100635742
+TSPAN6	CRISPRko	GTTCCTATTTTATTTCTAGCTGA	TTTT	0.7255	231	104	-	62	7	100630865
+TSPAN6	CRISPRko	GTTGTTTTGCTACCTGCCGAGCT	TTTG	0.6973	188	105	-	84	2	100635581
+TSPAN6	CRISPRko	TTTGGAGTTGCTTGCTTCCAAGT	TTCC	0.7209	226	106	-	66	6	100632485
+TSPAN6	CRISPRko	TGACTCTCGTTTTTTTGGTCGAA	TTTC	0.7688	277	107	-	43	3	100635219
+TSPAN6	CRISPRko	CAGTTGCATTGTTGTGGTGTCAC	TTTT	0.6406	150	108	-	107	5	100633522
+TSPAN6	CRISPRko	GGTGACACCACAACAATGCAACT	AATC	0.5372	24	109	+	173	5	100633540
+TSPAN6	CRISPRko	TCTACTGCATGGCTTCTATAATC	CTTG	0.5947	99	110	+	136	4	100633968
+TSPAN6	CRISPRko	TCTGAGTAATAATTAGTATCTGT	TTTT	0.6386	154	111	+	109	5	100633502
+TSPAN6	CRISPRko	GTCTCTCACCCAGAAAATAAAAG	CTTC	0.616	124	112	+	125	1	100636620
+TSPAN6	CRISPRko	AAGGTGATGACCATTATAGAGTC	TATA	0.6795	193	113	-	92	6	100632536
+TSPAN6	CRISPRko	AGAACAGCTTTAAGAATAATTAT	ATTA	0.7841	307	114	-	35	4	100634005
+TSPAN6	CRISPRko	ATAAAGGTGATGACCATTATAGA	TTTT	0.7636	287	115	-	46	6	100632539
+TSPAN6	CRISPRko	TATTTTATTTCTAGCTGATTGGA	TTCC	0.7721	296	116	-	42	7	100630860
+TSPAN6	CRISPRko	TCTCTCACCCAGAAAATAAAAGT	TTCG	0.6201	145	117	+	120	1	100636621
+TSPAN6	CRISPRko	TTCCTCCCATTAGGGTTGTTTTA	TTTT	0.7167	249	118	-	70	6	100632561
+TSPAN6	CRISPRko	TCCCATTAGGGTTGTTTTATAAA	TTCC	0.7051	233	119	-	79	6	100632557
+TSPAN6	CRISPRko	TTGGGTTTTTCAGTTGCATTGTT	TTTG	0.7343	283	120	-	57	5	100633532
+TSPAN6	CRISPRko	CCAGGCTCACCTTGCCCCAAATG	TTCT	0.6578	201	121	+	98	2	100635716
+TSPAN6	CRISPRko	ACCGATTATAGAGATTGGACAGA	TGTC	0.6153	148	122	-	126	5	100633501
+TSPAN6	CRISPRko	TTCCTATTTTATTTCTAGCTGAT	TTTG	0.7524	302	123	-	50	7	100630864
+TSPAN6	CRISPRko	GCTAATCTACACTTTTATTTTCT	TTCT	0.5958	135	124	-	134	1	100636612
+TSPAN6	CRISPRko	TATCCTGCAGTATGCAATGTTTC	TTCT	0.6278	175	125	-	115	3	100635242
+TSPAN6	CRISPRko	CGCCTACTGCCTCTCTCGTGCCA	TTCT	0.5216	38	126	-	187	7	100630829
+TSPAN6	CRISPRko	CCAAATGCCAACTGCAAGAAGGA	TGCC	0.5649	98	127	+	157	2	100635732
+TSPAN6	CRISPRko	ATAACAAATAACCAGTATGAGAT	TGCC	0.6769	227	128	-	93	7	100630807
+TSPAN6	CRISPRko	ACTTACCGTATTTTGGATCTTGT	TACC	0.6453	205	129	+	105	4	100633946
+TSPAN6	CRISPRko	GAATCTTTCTCGCCTACTGCCTC	ATTG	0.5056	19	130	-	200	7	100630839
+TSPAN6	CRISPRko	TCAGTTGCATTGTTGTGGTGTCA	TTTT	0.6656	229	131	-	96	5	100633523
+TSPAN6	CRISPRko	GATTAAGAACAGCTTTAAGAATA	TATA	0.5269	64	132	-	179	4	100634010
+TSPAN6	CRISPRko	GTTGGCATTTGGGGCAAGGTGAG	TGCA	0.524	55	133	-	184	2	100635702
+TSPAN6	CRISPRko	CAGTTGGCATTTGGGGCAAGGTG	CTTG	0.5688	118	134	-	154	2	100635704
+TSPAN6	CRISPRko	CATTGTTGTGGTGTCACCGATTA	GTTG	0.5006	20	135	-	204	5	100633516
+TSPAN6	CRISPRko	GGGTCTCGGGCTAGTCATGGCGT	TGCA	0.4866	6	136	-	213	1	100636690
+TSPAN6	CRISPRko	TTACTCAGAAAAAGGATTTCCTA	ATTA	0.7007	272	137	-	83	5	100633469
+TSPAN6	CRISPRko	AGGCTCACCTTGCCCCAAATGCC	CTCC	0.5898	160	138	+	140	2	100635718
+TSPAN6	CRISPRko	TTGGGTTACACTATCTCATACTG	TACA	0.5596	122	139	+	160	7	100630814
+TSPAN6	CRISPRko	ATTTCTAGCTGATTGGAATCTTT	TTTT	0.667	254	140	-	95	7	100630854
+TSPAN6	CRISPRko	TGCATCTCTCTGTGGAGTACAAT	TGTC	0.528	95	141	+	176	5	100633444
+TSPAN6	CRISPRko	TTCTTATCCTGCAGTATGCAATG	TTCT	0.5433	105	142	-	171	3	100635246
+TSPAN6	CRISPRko	AACTGCAAGAAGGATAACGCCAG	TGCC	0.551	115	143	+	166	2	100635741
+TSPAN6	CRISPRko	ATGGCGTCCCCGTCTCGGAGACT	AGTC	0.4805	7	144	-	220	1	100636674
+TSPAN6	CRISPRko	GGCCCACAGATACATTGGGTTAC	AATA	0.4906	32	145	+	208	7	100630800
+TSPAN6	CRISPRko	CCGTATTTTGGATCTTGTCTACT	CTTA	0.6198	206	146	+	121	4	100633951
+TSPAN6	CRISPRko	GTCTCGGAGACTGCAGACTAAAC	CCCC	0.4813	10	147	-	219	1	100636663
+TSPAN6	CRISPRko	TGCAGTATGCAATGTTTCTGACT	ATCC	0.5144	67	148	-	191	3	100635237
+TSPAN6	CRISPRko	TTTTATAAAGGTGATGACCATTA	GTTG	0.5629	134	149	-	158	6	100632543
+TSPAN6	CRISPRko	GATTATAGAGATTGGACAGATAC	CACC	0.5035	48	150	-	202	5	100633498
+TSPAN6	CRISPRko	CTGCAGTATGCAATGTTTCTGAC	TATC	0.5719	157	151	-	149	3	100635238
+TSPAN6	CRISPRko	TACTCCACAGAGAGATGCAGACA	ATTG	0.5917	183	152	-	137	5	100633421
+TSPAN6	CRISPRko	AGAAGGCTTTGAAGCAGTATAAC	TATG	0.5784	164	153	-	147	4	100633981
+TSPAN6	CRISPRko	AAACAAGTAATGACTGGTTTAGT	CTTG	0.619	217	154	+	122	1	100636666
+TSPAN6	CRISPRko	GTTGCATTGTTGTGGTGTCACCG	TTCA	0.6334	243	155	-	112	5	100633520
+TSPAN6	CRISPRko	GGGTTGTTTTATAAAGGTGATGA	ATTA	0.4921	56	156	-	207	6	100632549
+TSPAN6	CRISPRko	ACTACAAAAACTTACTTGGAAGC	AGTA	0.5297	123	157	+	175	6	100632491
+TSPAN6	CRISPRko	TCATGTCTGAAAACAAATCCTAC	TACC	0.6524	271	158	+	101	3	100635200
+TSPAN6	CRISPRko	AGGCGCCTTCGTCTCTCACCCAG	CCCC	0.4776	27	159	+	223	1	100636610
+TSPAN6	CRISPRko	CCCGTCTCGGAGACTGCAGACTA	CGTC	0.5269	111	160	-	181	1	100636666
+TSPAN6	CRISPRko	ATCTCTATAATCGGTGACACCAC	TCCA	0.4713	15	161	+	230	5	100633528
+TSPAN6	CRISPRko	CCTTCGTGCTCATTGCTACTGGT	GTCC	0.507	84	162	-	197	2	100635632
+TSPAN6	CRISPRko	ACAGAGAGATGCAGACAAAGTAA	CTCC	0.6168	239	163	-	123	5	100633415
+TSPAN6	CRISPRko	GGTTACACTATCTCATACTGGTT	ATTG	0.46	14	164	+	238	7	100630817
+TSPAN6	CRISPRko	TTGTTGTGGTGTCACCGATTATA	TGCA	0.4714	36	165	-	228	5	100633514
+TSPAN6	CRISPRko	GCACGAGAGAGGCAGTAGGCGAG	TATG	0.5558	173	166	+	163	7	100630850
+TSPAN6	CRISPRko	GTAGACAAGATCCAAAATACGGT	TGCA	0.5689	195	167	-	153	4	100633931
+TSPAN6	CRISPRko	CTATTTTATTTCTAGCTGATTGG	GTTC	0.6451	290	168	-	106	7	100630861
+TSPAN6	CRISPRko	CTTTTCTCTTTTAAATGAGAAGG	ATTA	0.6655	308	169	-	97	2	100635667
+TSPAN6	CRISPRko	AAATGAGAAGGCCACCAATGTCC	TTTT	0.565	191	170	-	156	2	100635655
+TSPAN6	CRISPRko	CTTTTAAATGAGAAGGCCACCAA	TTCT	0.6164	255	171	-	124	2	100635660
+TSPAN6	CRISPRko	GTATTTTGGATCTTGTCTACTGC	TACC	0.5869	219	172	+	143	4	100633953
+TSPAN6	CRISPRko	TTGCTATAGATTAAGAACAGCTT	TTCT	0.5615	187	173	-	159	4	100634018
+TSPAN6	CRISPRko	ATGCAGTAGACAAGATCCAAAAT	AGCC	0.5435	170	174	-	169	4	100633936
+TSPAN6	CRISPRko	ATGCAGAAGCTCGGCAGGTAGCA	ATCC	0.4863	79	175	+	215	2	100635593
+TSPAN6	CRISPRko	ATAATCTCCTGTAGAGTTATACT	TTCT	0.4661	44	176	+	233	4	100633985
+TSPAN6	CRISPRko	GTGCTCATTGCTACTGGTACCGT	CTTC	0.5487	178	177	-	167	2	100635627
+TSPAN6	CRISPRko	TTTTTTTCCTCCCATTAGGGTTG	TTTG	0.6313	286	178	-	114	6	100632566
+TSPAN6	CRISPRko	CTGTAAACTTGAAGATTGTACTC	GTTG	0.6121	258	179	-	128	5	100633439
+TSPAN6	CRISPRko	AAGCCTTCTCATAATTATTCTTA	TTCA	0.5948	251	180	+	135	4	100634014
+TSPAN6	CRISPRko	ACCAAAAAAACGAGAGTCAGAAA	TTCG	0.6263	289	181	+	116	3	100635242
+TSPAN6	CRISPRko	AAGATTGTACTCCACAGAGAGAT	CTTG	0.5978	256	182	-	133	5	100633428
+TSPAN6	CRISPRko	TCTGTCCAATCTCTATAATCGGT	AGTA	0.4864	97	183	+	214	5	100633520
+TSPAN6	CRISPRko	TGAAAACAAATCCTACGATGGCA	TGTC	0.5911	250	184	+	138	3	100635207
+TSPAN6	CRISPRko	GAGCTTCTGCATGGATGCTAAAA	TGCC	0.4475	33	185	-	247	2	100635563
+TSPAN6	CRISPRko	CCGATTATAGAGATTGGACAGAT	GTCA	0.4338	18	186	-	256	5	100633500
+TSPAN6	CRISPRko	TAATTCAGGCTTACCTCATGTCT	TCCC	0.5993	269	187	+	132	3	100635185
+TSPAN6	CRISPRko	ATTTCTGACTCTATAATGGTCAT	TCCC	0.5072	142	188	+	196	6	100632549
+TSPAN6	CRISPRko	TGGCGTCCCCGTCTCGGAGACTG	GTCA	0.4268	9	189	-	263	1	100636673
+TSPAN6	CRISPRko	AGTTTTAGCATCCATGCAGAAGC	CACC	0.4879	119	190	+	212	2	100635580
+TSPAN6	CRISPRko	ATCGGTGACACCACAACAATGCA	TATA	0.4835	110	191	+	217	5	100633537
+TSPAN6	CRISPRko	TTTTGCTACCTGCCGAGCTTCTG	GTTG	0.449	53	192	-	246	2	100635577
+TSPAN6	CRISPRko	ATGGTCATCACCTTTATAAAACA	TATA	0.5278	192	193	+	177	6	100632564
+TSPAN6	CRISPRko	TCTCTCGTGCCATAACAAATAAC	TGCC	0.5245	181	194	-	183	7	100630818
+TSPAN6	CRISPRko	AAATGCCAACTGCAAGAAGGATA	CCCC	0.5459	211	195	+	168	2	100635734
+TSPAN6	CRISPRko	GACAGATACTAATTATTACTCAG	ATTG	0.5749	252	196	-	148	5	100633484
+TSPAN6	CRISPRko	CCCTTCGTGCTCATTGCTACTGG	TGTC	0.5269	190	197	-	180	2	100635633
+TSPAN6	CRISPRko	TAGAGTCAGAAATGGGAGTCGTT	ATTA	0.5107	163	198	-	194	6	100632520
+TSPAN6	CRISPRko	ATCTCCTGTAGAGTTATACTGCT	TATA	0.4706	93	199	+	231	4	100633988
+TSPAN6	CRISPRko	ATCGTAGGATTTGTTTTCAGACA	TGCC	0.4515	71	200	-	243	3	100635184
+TSPAN6	CRISPRko	CCTTGCCCCAAATGCCAACTGCA	CTCA	0.501	151	201	+	203	2	100635725
+TSPAN6	CRISPRko	GTTGGGTTTTTCAGTTGCATTGT	TTTT	0.5321	220	202	-	174	5	100633533
+TSPAN6	CRISPRko	GAGTTATACTGCTTCAAAGCCTT	TGTA	0.4291	52	203	+	258	4	100633998
+TSPAN6	CRISPRko	AAAGTGTAGATTAGCAGAACGCT	AATA	0.5588	246	204	+	161	1	100636639
+TSPAN6	CRISPRko	CTCCACAGAGAGATGCAGACAAA	TGTA	0.5571	245	205	-	162	5	100633419
+TSPAN6	CRISPRko	TACTGCATGGCTTCTATAATCTC	TGTC	0.5196	198	206	+	188	4	100633970
+TSPAN6	CRISPRko	TTTTGGGCACCTTTGGTTGTTTT	ATTC	0.5704	275	207	-	150	2	100635596
+TSPAN6	CRISPRko	CTTCTTGCAGTTGGCATTTGGGG	TATC	0.4613	106	208	-	236	2	100635711
+TSPAN6	CRISPRko	ATTAGGGTTGTTTTATAAAGGTG	TCCC	0.5416	235	209	-	172	6	100632553
+TSPAN6	CRISPRko	CATTTCTGACTCTATAATGGTCA	CTCC	0.5875	297	210	+	142	6	100632548
+TSPAN6	CRISPRko	AACTTGAAGATTGTACTCCACAG	TGTA	0.4279	65	211	-	260	5	100633434
+TSPAN6	CRISPRko	TGCAGTCTCCGAGACGGGGACGC	AGTC	0.4008	25	212	+	281	1	100636690
+TSPAN6	CRISPRko	TTTTTCTGAGTAATAATTAGTAT	ATCC	0.5904	310	213	+	139	5	100633498
+TSPAN6	CRISPRko	GTGGCCTTCTCATTTAAAAGAGA	ATTG	0.4286	70	214	+	259	2	100635682
+TSPAN6	CRISPRko	CTGCTTCAAAGCCTTCTCATAAT	TATA	0.5557	261	215	+	164	4	100634006
+TSPAN6	CRISPRko	TTCGTGCTCATTGCTACTGGTAC	CCCC	0.4675	125	216	-	232	2	100635630
+TSPAN6	CRISPRko	TGTTTCTTTGCTATAGATTAAGA	ATTG	0.4458	100	217	-	249	4	100634025
+TSPAN6	CRISPRko	ATGACGGTACCAGTAGCAATGAG	AATA	0.5196	222	218	+	189	2	100635643
+TSPAN6	CRISPRko	TTCGTCTCTCACCCAGAAAATAA	CGCC	0.4157	68	219	+	269	1	100636617
+TSPAN6	CRISPRko	AATGTCCCCTTCGTGCTCATTGC	CACC	0.4362	101	220	-	254	2	100635639
+TSPAN6	CRISPRko	CTATCTCATACTGGTTATTTGTT	TACA	0.5266	248	221	+	182	7	100630824
+TSPAN6	CRISPRko	TCTCACCCAGAAAATAAAAGTGT	CGTC	0.522	241	222	+	186	1	100636623
+TSPAN6	CRISPRko	GTCTCCGAGACGGGGACGCCATG	TGCA	0.3231	2	223	+	306	1	100636694
+TSPAN6	CRISPRko	TTGCTACTGGTACCGTCATTATT	CTCA	0.4713	159	224	-	229	2	100635620
+TSPAN6	CRISPRko	ATAATTAGTATCTGTCCAATCTC	AGTA	0.4815	184	225	+	218	5	100633510
+TSPAN6	CRISPRko	CTACGATGGCAGCGACCAGTTCG	AATC	0.4728	166	226	+	227	3	100635219
+TSPAN6	CRISPRko	GTCATTATTCTTTTGGGCACCTT	TACC	0.3934	49	227	-	286	2	100635606
+TSPAN6	CRISPRko	TTGTGGTGTCACCGATTATAGAG	ATTG	0.4471	126	228	-	248	5	100633511
+TSPAN6	CRISPRko	GCAACTCTTAGGAAATCCTTTTT	TACA	0.4904	204	229	+	210	5	100633480
+TSPAN6	CRISPRko	CCCCAAATGCCAACTGCAAGAAG	CTTG	0.4244	96	230	+	265	2	100635730
+TSPAN6	CRISPRko	GGTTTTTCAGTTGCATTGTTGTG	GTTG	0.5435	292	231	-	170	5	100633529
+TSPAN6	CRISPRko	TGCTAATCTACACTTTTATTTTC	GTTC	0.5272	276	232	-	178	1	100636613
+TSPAN6	CRISPRko	GACTAAACCAGTCATTACTTGTT	TGCA	0.326	26	233	-	303	1	100636648
+TSPAN6	CRISPRko	GACTCTCGTTTTTTTGGTCGAAC	TTCT	0.4272	114	234	-	261	3	100635218
+TSPAN6	CRISPRko	TTGTTTACTTTGTCTGCATCTCT	TTCA	0.506	242	235	+	199	5	100633430
+TSPAN6	CRISPRko	TGCTCATTGCTACTGGTACCGTC	TTCG	0.4736	189	236	-	226	2	100635626
+TSPAN6	CRISPRko	TCATTTAAAAGAGAAAAGTAATT	CTTC	0.555	311	237	+	165	2	100635691
+TSPAN6	CRISPRko	ATCTTCAAGTTTACAGCAACTCT	TACA	0.4406	140	238	+	251	5	100633465
+TSPAN6	CRISPRko	CTGGCGTTATCCTTCTTGCAGTT	ATCA	0.4405	138	239	-	252	2	100635722
+TSPAN6	CRISPRko	GAGTACAATCTTCAAGTTTACAG	TGTG	0.3678	57	240	+	293	5	100633458
+TSPAN6	CRISPRko	TTTGGGCACCTTTGGTTGTTTTG	TTCT	0.5045	247	241	-	201	2	100635595
+TSPAN6	CRISPRko	ACGACTCCCATTTCTGACTCTAT	TGCA	0.316	39	242	+	307	6	100632540
+TSPAN6	CRISPRko	TTCTCTGAATTCCCACATAGATC	TGTA	0.5118	267	243	-	193	2	100635746
+TSPAN6	CRISPRko	AGCTGATTGGAATCTTTCTCGCC	TTCT	0.4906	237	244	-	209	7	100630848
+TSPAN6	CRISPRko	TAACTCTACAGGAGATTATAGAA	AGTA	0.3589	62	245	-	297	4	100633962
+TSPAN6	CRISPRko	CCGTCTCGGAGACTGCAGACTAA	GTCC	0.4519	172	246	-	242	1	100636665
+TSPAN6	CRISPRko	TGGAGAATTACTTTTCTCTTTTA	AGCC	0.5095	268	247	-	195	2	100635677
+TSPAN6	CRISPRko	CTCTTTTAAATGAGAAGGCCACC	TTTT	0.5234	298	248	-	185	2	100635662
+TSPAN6	CRISPRko	GGGCACCTTTGGTTGTTTTGCTA	TTTT	0.38	91	249	-	289	2	100635592
+TSPAN6	CRISPRko	ATTAGTATCTGTCCAATCTCTAT	AATA	0.4498	182	250	+	245	5	100633513
+TSPAN6	CRISPRko	TACTGCCTCTCTCGTGCCATAAC	CGCC	0.4758	224	251	-	225	7	100630825
+TSPAN6	CRISPRko	GAAATGGGAGTCGTTGCAGGAAT	GTCA	0.4795	232	252	-	221	6	100632512
+TSPAN6	CRISPRko	TCTATAATCGGTGACACCACAAC	AATC	0.3515	77	253	+	300	5	100633531
+TSPAN6	CRISPRko	GTTGCAGGAATTTCCTTTGGAGT	AGTC	0.5069	284	254	-	198	6	100632500
+TSPAN6	CRISPRko	GAAAAAGGATTTCCTAAGAGTTG	CTCA	0.5158	301	255	-	190	5	100633462
+TSPAN6	CRISPRko	TGCAGAAGCTCGGCAGGTAGCAA	TCCA	0.4304	168	256	+	257	2	100635594
+TSPAN6	CRISPRko	TCATAATTATTCTTAAAGCTGTT	CTTC	0.5142	300	257	+	192	4	100634022
+TSPAN6	CRISPRko	TCATACTGGTTATTTGTTATGGC	TATC	0.4861	257	258	+	216	7	100630829
+TSPAN6	CRISPRko	ATTCTCCAGGCTCACCTTGCCCC	AGTA	0.4538	208	259	+	241	2	100635711
+TSPAN6	CRISPRko	CATTAGGGTTGTTTTATAAAGGT	CTCC	0.41	146	260	-	273	6	100632554
+TSPAN6	CRISPRko	TCGGAGACTGCAGACTAAACCAG	CGTC	0.4272	174	261	-	262	1	100636660
+TSPAN6	CRISPRko	GGAGATTATAGAAGCCATGCAGT	TACA	0.4064	149	262	-	275	4	100633952
+TSPAN6	CRISPRko	CAATCTCTATAATCGGTGACACC	TGTC	0.4109	161	263	+	272	5	100633526
+TSPAN6	CRISPRko	CTTCATTGTTTACTTTGTCTGCA	CTTA	0.4949	303	264	+	206	5	100633425
+TSPAN6	CRISPRko	AGTCATTACTTGTTTCAAGAGCG	AACC	0.4896	293	265	-	211	1	100636639
+TSPAN6	CRISPRko	ATTATTCTTTTGGGCACCTTTGG	CGTC	0.2992	92	266	-	312	2	100635603
+TSPAN6	CRISPRko	CAGAAAATAAAAGTGTAGATTAG	CACC	0.4962	309	267	+	205	1	100636630
+TSPAN6	CRISPRko	TAATTATTCTTAAAGCTGTTCTT	CTCA	0.4549	240	268	+	240	4	100634025
+TSPAN6	CRISPRko	GGAATTTCCTTTGGAGTTGCTTG	TGCA	0.4258	194	269	-	264	6	100632494
+TSPAN6	CRISPRko	AAGGAAATTCCTGCAACGACTCC	TCCA	0.3776	144	270	+	290	6	100632525
+TSPAN6	CRISPRko	AAAGGTGCCCAAAAGAATAATGA	AACC	0.451	236	271	+	244	2	100635624
+TSPAN6	CRISPRko	AGAAATGGGAGTCGTTGCAGGAA	AGTC	0.4072	177	272	-	274	6	100632513
+TSPAN6	CRISPRko	CTTGTTTCAAGAGCGTTCTGCTA	ATTA	0.3968	162	273	-	283	1	100636631
+TSPAN6	CRISPRko	ATGACTGGTTTAGTCTGCAGTCT	AGTA	0.4647	260	274	+	234	1	100636675
+TSPAN6	CRISPRko	TTTCTCGCCTACTGCCTCTCTCG	AATC	0.356	132	275	-	299	7	100630834
+TSPAN6	CRISPRko	TTTCTTTGCTATAGATTAAGAAC	TGTG	0.4768	282	276	-	224	4	100634023
+TSPAN6	CRISPRko	TTCTTGCAGTTGGCATTTGGGGC	ATCC	0.3946	165	277	-	285	2	100635710
+TSPAN6	CRISPRko	GGCTCACCTTGCCCCAAATGCCA	TCCA	0.2913	113	278	+	313	2	100635719
+TSPAN6	CRISPRko	CCCAGAAAATAAAAGTGTAGATT	CTCA	0.4633	270	279	+	235	1	100636628
+TSPAN6	CRISPRko	TGCCGAGCTTCTGCATGGATGCT	TACC	0.411	199	280	-	271	2	100635567
+TSPAN6	CRISPRko	TACTGGTTATTTGTTATGGCACG	CTCA	0.423	212	281	+	266	7	100630832
+TSPAN6	CRISPRko	CCAGTTTTAGCATCCATGCAGAA	CTCA	0.4605	273	282	+	237	2	100635578
+TSPAN6	CRISPRko	TTTCAAGAGCGTTCTGCTAATCT	CTTG	0.4355	238	283	-	255	1	100636627
+TSPAN6	CRISPRko	CAATCTTCAAGTTTACAGCAACT	AGTA	0.3804	180	284	+	288	5	100633463
+TSPAN6	CRISPRko	CAATGTTTCTGACTCTCGTTTTT	TATG	0.4212	225	285	-	267	3	100635228
+TSPAN6	CRISPRko	TTTAAAAGAGAAAAGTAATTCTC	CTCA	0.4781	317	286	+	222	2	100635694
+TSPAN6	CRISPRko	TTCAAGTTTACAGCAACTCTTAG	AATC	0.3032	143	287	+	310	5	100633468
+TSPAN6	CRISPRko	ATTACTTGTTTCAAGAGCGTTCT	AGTC	0.2804	137	288	-	314	1	100636635
+TSPAN6	CRISPRko	TGCAATGTTTCTGACTCTCGTTT	AGTA	0.2431	139	289	-	315	3	100635230
+TSPAN6	CRISPRko	TTGCCCCAAATGCCAACTGCAAG	CACC	0.4372	265	290	+	253	2	100635727
+TSPAN6	CRISPRko	TCTCTCTGTGGAGTACAATCTTC	TGCA	0.31	158	291	+	308	5	100633448
+TSPAN6	CRISPRko	TGCAGTTGGCATTTGGGGCAAGG	TTCT	0.3009	156	292	-	311	2	100635706
+TSPAN6	CRISPRko	ATGTTTCTGACTCTCGTTTTTTT	TGCA	0.3996	216	293	-	282	3	100635226
+TSPAN6	CRISPRko	TTACTTCATTGTTTACTTTGTCT	CACC	0.3961	221	294	+	284	5	100633422
+TSPAN6	CRISPRko	CATTTAAAAGAGAAAAGTAATTC	TTCT	0.4567	312	295	+	239	2	100635692
+TSPAN6	CRISPRko	CTTTTTCTGAGTAATAATTAGTA	AATC	0.4407	291	296	+	250	5	100633497
+TSPAN6	CRISPRko	GTATGCAATGTTTCTGACTCTCG	TGCA	0.3614	202	297	-	296	3	100635233
+TSPAN6	CRISPRko	TTACTTGTTTCAAGAGCGTTCTG	GTCA	0.3641	207	298	-	295	1	100636634
+TSPAN6	CRISPRko	TCCTTCTTGCAGTTGGCATTTGG	GTTA	0.4014	244	299	-	280	2	100635713
+TSPAN6	CRISPRko	GGCGAGAAAGATTCCAATCAGCT	AGTA	0.3327	203	300	+	302	7	100630867
+TSPAN6	CRISPRko	CTACTGGTACCGTCATTATTCTT	ATTG	0.3666	223	301	-	294	2	100635617
+TSPAN6	CRISPRko	CATAATTATTCTTAAAGCTGTTC	TTCT	0.4057	262	302	+	276	4	100634023
+TSPAN6	CRISPRko	TGGCTTCTATAATCTCCTGTAGA	TGCA	0.3383	213	303	+	301	4	100633977
+TSPAN6	CRISPRko	TCCTGTAGAGTTATACTGCTTCA	AATC	0.4047	264	304	+	277	4	100633991
+TSPAN6	CRISPRko	GCAATGAGCACGAAGGGGACATT	AGTA	0.3063	200	305	+	309	2	100635658
+TSPAN6	CRISPRko	TTTGGTTGTTTTGCTACCTGCCG	CACC	0.403	266	306	-	278	2	100635585
+TSPAN6	CRISPRko	CAGAGAGATGCAGACAAAGTAAA	TCCA	0.4119	285	307	-	270	5	100633414
+TSPAN6	CRISPRko	ATTATGAGAAGGCTTTGAAGCAG	AATA	0.4025	279	308	-	279	4	100633987
+TSPAN6	CRISPRko	AGAAAATAAAAGTGTAGATTAGC	ACCC	0.4158	306	309	+	268	1	100636631
+TSPAN6	CRISPRko	TTATTCTTTTGGGCACCTTTGGT	GTCA	0.1456	234	310	-	317	2	100635602
+TSPAN6	CRISPRko	TTGCAGTTGGCATTTGGGGCAAG	CTTC	0.358	274	311	-	298	2	100635707
+TSPAN6	CRISPRko	TTCTCATAATTATTCTTAAAGCT	AGCC	0.3805	304	312	+	287	4	100634019
+TSPAN6	CRISPRko	TGTCTGAAAACAAATCCTACGAT	CTCA	0.3716	299	313	+	291	3	100635203
+TSPAN6	CRISPRko	CAGGAATTTCCTTTGGAGTTGCT	GTTG	0.3684	316	314	-	292	6	100632496
+TSPAN6	CRISPRko	TTCTTTTGGGCACCTTTGGTTGT	ATTA	0.3251	294	315	-	304	2	100635599
+TSPAN6	CRISPRko	AAAACTTACTTGGAAGCAAGCAA	TACA	0.3232	295	316	+	305	6	100632497
+TSPAN6	CRISPRko	TTTACTTTGTCTGCATCTCTCTG	ATTG	0.2012	315	317	+	316	5	100633433
+TNMD	CRISPRko	CTAGGCTACTACCCATATCCATA	TTTA	0.8913	33	1	+	11	7	100599634
+TNMD	CRISPRko	AAGACCCACGAAGTAGATGCCAG	TTTG	0.9001	39	2	-	8	4	100594270
+TNMD	CRISPRko	GTATCCTGGCCCTAACTCTAATT	TTTG	0.875	18	3	+	19	2	100585313
+TNMD	CRISPRko	GGGGAGCAAGCACTTCTGGCCGG	TTTG	0.8849	56	4	+	14	2	100585347
+TNMD	CRISPRko	TAGGGATACACTGGCATCTACTT	TTTA	0.9819	84	5	+	1	4	100594279
+TNMD	CRISPRko	AGCTCAAACCTCCTATTAGACCC	TTTG	0.8512	38	6	-	25	7	100599712
+TNMD	CRISPRko	CAAGGCATGATGACACGACAGAT	GTTA	0.8201	26	7	-	36	7	100599660
+TNMD	CRISPRko	AGTTCTGGTCACAGGATCAATTT	TTTC	0.8835	85	8	-	15	3	100593955
+TNMD	CRISPRko	ACAGACGCGGCGGCAATAGCGGT	GTTC	0.779	4	9	-	57	7	100599583
+TNMD	CRISPRko	GGTACCTCCGGCCAGAAGTGCTT	TTTG	0.7884	15	10	-	52	2	100585335
+TNMD	CRISPRko	GAGTTAGGGCCAGGATACCAAAC	ATTA	0.7931	24	11	-	48	2	100585289
+TNMD	CRISPRko	GGGCCAGGATACCAAACACCAGT	GTTA	0.8268	52	12	-	34	2	100585283
+TNMD	CRISPRko	AAGTCGTGCACTTCCAATGTTTC	TTTA	0.8614	80	13	-	21	3	100594008
+TNMD	CRISPRko	TGAGTTACAAGACTTTGAGGAGG	TTTC	0.8868	101	14	+	12	6	100599041
+TNMD	CRISPRko	GTTGGCAGGAAAGTGAAGATCTT	TTTC	0.9528	118	15	-	4	6	100599051
+TNMD	CRISPRko	TGATAACGTGACCATGTATTGGA	TTTG	0.8317	62	16	+	32	5	100597635
+TNMD	CRISPRko	GAAGACCCACGAAGTAGATGCCA	TTTT	0.7887	30	17	-	51	4	100594271
+TNMD	CRISPRko	TGCTGGGACCCAAATCACTGACT	TTTC	0.9461	123	18	-	5	5	100597537
+TNMD	CRISPRko	GAATGTGACAGTCTTCACAATTC	TTTA	0.886	110	19	-	13	1	100585041
+TNMD	CRISPRko	ATCAGTGCCATTTCCGCTTCTGA	TTTC	0.8058	54	20	-	42	3	100593985
+TNMD	CRISPRko	TGAAGACCCACGAAGTAGATGCC	TTTT	0.792	41	21	-	49	4	100594272
+TNMD	CRISPRko	TGGACTGGTGTTTGGTATCCTGG	TTTG	0.8193	66	22	+	37	2	100585299
+TNMD	CRISPRko	GAAGCGGAAATGGCACTGATGAA	TTCA	0.7666	25	23	+	63	3	100594007
+TNMD	CRISPRko	TCAAAACTCAGATTAAAGTGATT	TTTA	0.8966	132	24	+	10	4	100594325
+TNMD	CRISPRko	CTGCCGTCGAGGCAACCGCTATT	TTTA	0.843	103	25	+	27	7	100599588
+TNMD	CRISPRko	TGGCCGGAGGTACCCAAAAAAGT	CTTC	0.7681	50	26	+	62	2	100585363
+TNMD	CRISPRko	GTTTCTGAGTTACAAGACTTTGA	CTTA	0.779	70	27	+	56	6	100599036
+TNMD	CRISPRko	CATGTAAATCTTCTTCTTCTCTC	TTTC	0.8803	151	28	-	17	3	100593931
+TNMD	CRISPRko	TGGGTACCTCCGGCCAGAAGTGC	TTTT	0.7787	81	29	-	58	2	100585337
+TNMD	CRISPRko	GACCCTCCCCAGCATGCGGGCCA	ATTA	0.7169	11	30	-	99	7	100599694
+TNMD	CRISPRko	GGGTACCTCCGGCCAGAAGTGCT	TTTT	0.7179	16	31	-	97	2	100585336
+TNMD	CRISPRko	GAGTGGGATTGATCCAATACATG	ATTA	0.7339	51	32	-	80	5	100597628
+TNMD	CRISPRko	GGGGGAGCAAGCACTTCTGGCCG	TTTT	0.726	58	33	+	84	2	100585346
+TNMD	CRISPRko	CTTGAGGGACCACCCACTGTTCA	TTCA	0.7241	65	34	-	86	6	100599097
+TNMD	CRISPRko	ATAGGGATACACTGGCATCTACT	TTTT	0.8563	191	35	+	23	4	100594278
+TNMD	CRISPRko	CCTCGACGGCAGTAAATACAACA	GTTG	0.6958	12	36	-	113	7	100599558
+TNMD	CRISPRko	AAAGTCGTGCACTTCCAATGTTT	TTTT	0.8299	173	37	-	33	3	100594009
+TNMD	CRISPRko	CTCTGTTCTCCCAGGCCTATGAC	TTTC	0.7991	154	38	+	45	3	100593902
+TNMD	CRISPRko	TCGTTGGCAGGAAAGTGAAGATC	TTTT	0.727	95	39	-	82	6	100599053
+TNMD	CRISPRko	ACTTGAGGGACCACCCACTGTTC	TTTC	0.8831	229	40	-	16	6	100599098
+TNMD	CRISPRko	GGTCCCAGCAGAAAAGCCTATTG	TTTG	0.8564	222	41	+	22	5	100597569
+TNMD	CRISPRko	AGAAGCGGAAATGGCACTGATGA	ATTC	0.7494	120	42	+	73	3	100594006
+TNMD	CRISPRko	CATGGAAATTGATCCTGTGACCA	TTTA	0.8994	248	43	+	9	3	100593969
+TNMD	CRISPRko	TCCCAGGCCTATGACATGGAGCA	GTTC	0.72	88	44	+	95	3	100593910
+TNMD	CRISPRko	TTGGAAGTTCTTCCTCACTTGCT	TTTA	0.7607	152	45	-	66	6	100599151
+TNMD	CRISPRko	AATGTTTCATCAGTGCCATTTCC	TTCC	0.7247	116	46	-	85	3	100593993
+TNMD	CRISPRko	TGCCAACGAAAAAAAAGGGATTG	TTCC	0.7619	157	47	+	65	6	100599086
+TNMD	CRISPRko	ATCCCATGCTGGATGAGAGAGGT	TTTG	0.8378	232	48	+	29	7	100599551
+TNMD	CRISPRko	ACTTTCCTGCCAACGAAAAAAAA	CTTC	0.7646	163	49	+	64	6	100599079
+TNMD	CRISPRko	AATAGGCTTTTCTGCTGGGACCC	TTTC	0.9131	280	50	-	6	5	100597549
+TNMD	CRISPRko	GCTTCTGAATATTTCAGTTCTGG	TTCC	0.7533	153	51	-	71	3	100593970
+TNMD	CRISPRko	GTCTGTTTTATAGGGATACACTG	TTTT	0.7109	91	52	+	103	4	100594269
+TNMD	CRISPRko	CTGGCCCTAACTCTAATTGTCCT	TATC	0.6805	57	53	+	122	2	100585318
+TNMD	CRISPRko	TCAGTGCCATTTCCGCTTCTGAA	TTCA	0.7399	144	54	-	79	3	100593984
+TNMD	CRISPRko	TCTGTTTTATAGGGATACACTGG	TTTG	0.8447	251	55	+	26	4	100594270
+TNMD	CRISPRko	TCGACGGCAGTAAATACAACAAT	TGCC	0.6484	14	56	-	146	7	100599556
+TNMD	CRISPRko	TCTGGCGTGACGGGTCTTCTCTA	CTTG	0.6392	8	57	-	152	6	100599126
+TNMD	CRISPRko	AAGTGATTCCTGAATTTTCTGAA	ATTA	0.6892	77	58	+	119	4	100594340
+TNMD	CRISPRko	GTGGGTCTTCAAAAATGTTTTAT	CTTC	0.7845	209	59	+	53	4	100594303
+TNMD	CRISPRko	ATCTGAGTTTTGATAAAACATTT	TTTA	0.8361	257	60	-	31	4	100594296
+TNMD	CRISPRko	AATCCAAGAAAATATGTAAATCA	TTTA	0.9732	316	61	+	2	2	100585265
+TNMD	CRISPRko	CTGCCAACGAAAAAAAAGGGATT	TTTC	0.8021	235	62	+	43	6	100599085
+TNMD	CRISPRko	ATCCAATACATGGTCACGTTATC	ATTG	0.6451	23	63	-	149	5	100597617
+TNMD	CRISPRko	TCTGTTCTCCCAGGCCTATGACA	TTCC	0.8067	245	64	+	41	3	100593903
+TNMD	CRISPRko	TAAGAAAATCTCGGTTTTCAATA	TTTT	0.8008	240	65	-	44	5	100597568
+TNMD	CRISPRko	TGTGACCAGAACTGAAATATTCA	ATCC	0.7132	126	66	+	101	3	100593984
+TNMD	CRISPRko	GTTCTGGTCACAGGATCAATTTC	TTCA	0.7407	172	67	-	78	3	100593954
+TNMD	CRISPRko	CTTTTTTGGGTACCTCCGGCCAG	CTTA	0.6548	48	68	-	141	2	100585343
+TNMD	CRISPRko	GATCAATCCCACTCTAATATCAG	ATTG	0.6305	21	69	+	158	5	100597656
+TNMD	CRISPRko	AATACATGGTCACGTTATCACAA	ATCC	0.6922	105	70	-	116	5	100597613
+TNMD	CRISPRko	CAGACGCGGCGGCAATAGCGGTT	TTCA	0.6177	5	71	-	168	7	100599582
+TNMD	CRISPRko	CTTACTTTTTTGGGTACCTCCGG	TTTA	0.7587	207	72	-	68	2	100585347
+TNMD	CRISPRko	CCACAACTTTCTTTGAACAGTCA	ATTA	0.7097	137	73	+	105	5	100597538
+TNMD	CRISPRko	CTTGTCTGGCGTGACGGGTCTTC	CTTG	0.6161	9	74	-	170	6	100599130
+TNMD	CRISPRko	GACTGAAAATGGAATAGAATTTG	TTCA	0.6918	117	75	+	117	7	100599528
+TNMD	CRISPRko	CGTTGGCAGGAAAGTGAAGATCT	TTTT	0.724	175	76	-	88	6	100599052
+TNMD	CRISPRko	CGCTTCTGAATATTTCAGTTCTG	TTTC	0.7111	148	77	-	102	3	100593971
+TNMD	CRISPRko	GTTAGGCAGAAGCTTTTAAATCC	TTTG	0.812	279	78	+	39	2	100585247
+TNMD	CRISPRko	CTTGGATTTAAAAGCTTCTGCCT	TTTT	0.6625	94	79	-	134	2	100585231
+TNMD	CRISPRko	ATCAAAACTCAGATTAAAGTGAT	TTTT	0.7239	185	80	+	89	4	100594324
+TNMD	CRISPRko	GAACCAGAAGAGGAAATAGATGA	TTCT	0.679	119	81	+	123	4	100594360
+TNMD	CRISPRko	ATAGGCTTTTCTGCTGGGACCCA	TTCA	0.6473	73	82	-	147	5	100597548
+TNMD	CRISPRko	GCCGCGTCTGTGAACCTTTACTA	TGCC	0.602	3	83	+	184	7	100599614
+TNMD	CRISPRko	TACAGCAATGGAGAGAAGAAGAA	TTTC	0.7904	273	84	+	50	3	100593940
+TNMD	CRISPRko	AGACTGAAAATGGAATAGAATTT	TTTC	0.8658	337	85	+	20	7	100599527
+TNMD	CRISPRko	ATGCTGGATGAGAGAGGTTATTG	TCCC	0.6388	72	86	+	153	7	100599556
+TNMD	CRISPRko	CAGGCCTATGACATGGAGCACAC	CTCC	0.6739	121	87	+	129	3	100593913
+TNMD	CRISPRko	GCTGGGACCCAAATCACTGACTG	TTCT	0.7062	169	88	-	107	5	100597536
+TNMD	CRISPRko	GATATGGGTAGTAGCCTAGTAAA	TATG	0.599	10	89	-	187	7	100599611
+TNMD	CRISPRko	ATTGCTGTAGAAAGTGTGCTCCA	CTCC	0.6771	135	90	-	125	3	100593907
+TNMD	CRISPRko	GGCAGAAGCTTTTAAATCCAAGA	GTTA	0.7264	219	91	+	83	2	100585251
+TNMD	CRISPRko	ATACTGCTACCAAGGAGGACGAG	ATCC	0.6697	124	92	+	131	7	100599654
+TNMD	CRISPRko	GGAGGAAAACATCCAACTTACGT	CTTA	0.7016	167	93	-	111	3	100594036
+TNMD	CRISPRko	TGTGAACCTTTACTAGGCTACTA	CGTC	0.6271	64	94	+	163	7	100599622
+TNMD	CRISPRko	CAATAGGCTTTTCTGCTGGGACC	TTTT	0.6938	162	95	-	114	5	100597550
+TNMD	CRISPRko	AGGAATCACTTTAATCTGAGTTT	ATTC	0.7527	249	96	-	72	4	100594309
+TNMD	CRISPRko	CTCTTCTGGTTCAGAAAATTCAG	TTTC	0.7955	302	97	-	47	4	100594330
+TNMD	CRISPRko	TTAGTTTCTGAGTTACAAGACTT	TATC	0.6295	75	98	+	161	6	100599033
+TNMD	CRISPRko	ACTGGTGGGTGGCCCGCATGCTG	TGTA	0.597	20	99	+	189	7	100599704
+TNMD	CRISPRko	CCGCCGCGTCTGTGAACCTTTAC	ATTG	0.5842	6	100	+	196	7	100599612
+TNMD	CRISPRko	TATTTACTGCCGTCGAGGCAACC	GTTG	0.602	31	101	+	185	7	100599582
+TNMD	CRISPRko	CATAAACATAACTCACATAGTCA	TTTA	0.9697	395	102	-	3	6	100599178
+TNMD	CRISPRko	TTCGTTGGCAGGAAAGTGAAGAT	TTTT	0.6999	177	103	-	112	6	100599054
+TNMD	CRISPRko	TAAAGTCGTGCACTTCCAATGTT	TTTT	0.717	205	104	-	98	3	100594010
+TNMD	CRISPRko	TTATCTTAGTTTCTGAGTTACAA	TTTA	0.8376	342	105	+	30	6	100599028
+TNMD	CRISPRko	TGAATTTTCTGAACCAGAAGAGG	TTCC	0.6786	155	106	+	124	4	100594350
+TNMD	CRISPRko	CATATTTTCTTGGATTTAAAAGC	TTTA	0.8517	355	107	-	24	2	100585239
+TNMD	CRISPRko	CATTTAGAATGTGACAGTCTTCA	CTTA	0.7457	255	108	-	75	1	100585047
+TNMD	CRISPRko	TCTCTGTTCTTTGGTTAGGCAGA	ATTC	0.6588	128	109	+	139	2	100585234
+TNMD	CRISPRko	AGAATGAAGAAATTACCACAACT	TTTC	0.8388	352	110	+	28	5	100597523
+TNMD	CRISPRko	AAACACCAGTCCACAAATCTTAA	TACC	0.6458	114	111	-	148	2	100585270
+TNMD	CRISPRko	TGAACCAGAAGAGGAAATAGATG	TTTC	0.9035	397	112	+	7	4	100594359
+TNMD	CRISPRko	GGAATCACTTTAATCTGAGTTTT	TTCA	0.7201	223	113	-	94	4	100594308
+TNMD	CRISPRko	ATAAAACATTTTTGAAGACCCAC	TTTG	0.8755	376	114	-	18	4	100594284
+TNMD	CRISPRko	ATGTAAATCTTCTTCTTCTCTCC	TTCC	0.7812	304	115	-	54	3	100593930
+TNMD	CRISPRko	TGATGTGTATTTACTTACTTTTT	ATTA	0.7411	259	116	-	77	2	100585360
+TNMD	CRISPRko	ATCCTGTGACCAGAACTGAAATA	ATTG	0.6321	109	117	+	156	3	100593980
+TNMD	CRISPRko	TCCCTCCTCCTCAAAGTCTTGTA	CTTC	0.6936	193	118	-	115	6	100599027
+TNMD	CRISPRko	AAAATTCTGGAGATTTGTGATAA	TTCC	0.7968	333	119	+	46	5	100597618
+TNMD	CRISPRko	AGTGATTTACATATTTTCTTGGA	CTTA	0.7781	312	120	-	59	2	100585248
+TNMD	CRISPRko	AGAAAATCTCGGTTTTCAATAGG	TTTA	0.8143	356	121	-	38	5	100597566
+TNMD	CRISPRko	CAAGACTTTGAGGAGGAGGGAGA	GTTA	0.7586	303	122	+	69	6	100599048
+TNMD	CRISPRko	TGGTCACAGGATCAATTTCCATG	GTTC	0.722	261	123	-	92	3	100593950
+TNMD	CRISPRko	CTGAACCAGAAGAGGAAATAGAT	TTTT	0.7724	326	124	+	61	4	100594358
+TNMD	CRISPRko	AACAGTCAGTGATTTGGGTCCCA	TTTG	0.6761	200	125	+	126	5	100597553
+TNMD	CRISPRko	TTGGGTACCTCCGGCCAGAAGTG	TTTT	0.5572	34	126	-	209	2	100585338
+TNMD	CRISPRko	GAATTTTTAAGAAAATCTCGGTT	TTTG	0.7331	291	127	-	81	5	100597575
+TNMD	CRISPRko	AAAGAAAGTTGTGGTAATTTCTT	GTTC	0.8106	374	128	-	40	5	100597510
+TNMD	CRISPRko	TCCGGCCAGAAGTGCTTGCTCCC	TACC	0.6647	188	129	-	133	2	100585329
+TNMD	CRISPRko	GAATGAAGAAATTACCACAACTT	TTCA	0.724	281	130	+	87	5	100597524
+TNMD	CRISPRko	TCTTCTGGTTCAGAAAATTCAGG	TTCC	0.7806	345	131	-	55	4	100594329
+TNMD	CRISPRko	CAATGTTTCATCAGTGCCATTTC	CTTC	0.6117	107	132	-	175	3	100593994
+TNMD	CRISPRko	CATACTGCTACCAAGGAGGACGA	TATC	0.6106	104	133	+	177	7	100599653
+TNMD	CRISPRko	AGGGACCACCCACTGTTCATTTT	CTTG	0.6744	203	134	-	128	6	100599093
+TNMD	CRISPRko	CGTTTTTAAAGTCGTGCACTTCC	CTTA	0.6487	170	135	-	145	3	100594016
+TNMD	CRISPRko	AGGAGGAGGGAGAAGATCTTCAC	TTTG	0.777	346	136	+	60	6	100599058
+TNMD	CRISPRko	TTAAAAATTCCAAAATTCTGGAG	TTTC	0.821	398	137	+	35	5	100597607
+TNMD	CRISPRko	GGGATACACTGGCATCTACTTCG	TATA	0.6227	134	138	+	167	4	100594281
+TNMD	CRISPRko	AAGAAAATCTCGGTTTTCAATAG	TTTT	0.7462	321	139	-	74	5	100597567
+TNMD	CRISPRko	ACGAAGTAGATGCCAGTGTATCC	ACCC	0.5513	35	140	-	217	4	100594263
+TNMD	CRISPRko	TTGGATCAATCCCACTCTAATAT	TGTA	0.6099	112	141	+	179	5	100597653
+TNMD	CRISPRko	GGAATTTTTAAGAAAATCTCGGT	TTTT	0.7022	253	142	-	110	5	100597576
+TNMD	CRISPRko	TGAAGACTGTCACATTCTAAATG	ATTG	0.6368	165	143	+	154	1	100585066
+TNMD	CRISPRko	TCTTAGTTTCTGAGTTACAAGAC	ATTA	0.6555	196	144	+	140	6	100599031
+TNMD	CRISPRko	CTGCTGGGACCCAAATCACTGAC	TTTT	0.6163	141	145	-	169	5	100597538
+TNMD	CRISPRko	CTCCCCCAAAACAGGACAATTAG	CTTG	0.5809	93	146	-	197	2	100585311
+TNMD	CRISPRko	TGGCGTGACGGGTCTTCTCTACT	TGTC	0.564	78	147	-	205	6	100599124
+TNMD	CRISPRko	GATAAAACATTTTTGAAGACCCA	TTTT	0.7598	354	148	-	67	4	100594285
+TNMD	CRISPRko	TGGCCCTAACTCTAATTGTCCTG	ATCC	0.63	176	149	+	160	2	100585319
+TNMD	CRISPRko	GAAGTGCACGACTTTAAAAACGT	ATTG	0.5491	59	150	+	219	3	100594036
+TNMD	CRISPRko	TTGGCAGGAAAGTGAAGATCTTC	TTCG	0.6905	265	151	-	118	6	100599050
+TNMD	CRISPRko	TGGTCACGTTATCACAAATCTCC	TACA	0.5345	37	152	-	233	5	100597607
+TNMD	CRISPRko	CATACCTCATCTATTTCCTCTTC	CTTA	0.6713	243	153	-	130	4	100594347
+TNMD	CRISPRko	GAAAATTCAGGAATCACTTTAAT	TTCA	0.6547	221	154	-	142	4	100594317
+TNMD	CRISPRko	TCTCCATTGCTGTAGAAAGTGTG	CTTC	0.705	289	155	-	108	3	100593912
+TNMD	CRISPRko	TTGTTGTATTTACTGCCGTCGAG	GTTA	0.7165	313	156	+	100	7	100599576
+TNMD	CRISPRko	ATACCTGATATTAGAGTGGGATT	TGTC	0.6039	147	157	-	183	5	100597641
+TNMD	CRISPRko	TGATATTAGAGTGGGATTGATCC	TACC	0.6003	146	158	-	186	5	100597636
+TNMD	CRISPRko	AGAAAATTCAGGAATCACTTTAA	GTTC	0.6836	276	159	-	121	4	100594318
+TNMD	CRISPRko	AGATTTGTGGACTGGTGTTTGGT	CTTA	0.6841	285	160	+	120	2	100585292
+TNMD	CRISPRko	TCACTTGCTTGTCTGGCGTGACG	TTCC	0.6317	215	161	-	157	6	100599137
+TNMD	CRISPRko	CATTTATTATCTTAGTTTCTGAG	TTTG	0.7449	380	162	+	76	6	100599022
+TNMD	CRISPRko	AAGAAAGTTGTGGTAATTTCTTC	TTCA	0.7235	350	163	-	91	5	100597509
+TNMD	CRISPRko	TCCAGAGAATTGTGAAGACTGTC	ATCC	0.5392	74	164	+	230	1	100585054
+TNMD	CRISPRko	TTTGAACAGTCAGTGATTTGGGT	TTTC	0.7235	357	165	+	90	5	100597549
+TNMD	CRISPRko	TTCTTTCAGAATGAAGAAATTAC	TTTC	0.7578	403	166	+	70	5	100597516
+TNMD	CRISPRko	AAAATTCCAAAATTCTGGAGATT	CTTA	0.7108	338	167	+	104	5	100597610
+TNMD	CRISPRko	TCTACTTTCACTTGAGGGACCAC	CTTC	0.6614	275	168	-	137	6	100599107
+TNMD	CRISPRko	GGTCACAGGATCAATTTCCATGT	TTCT	0.6097	189	169	-	180	3	100593949
+TNMD	CRISPRko	CTGAATTTTCTGAACCAGAAGAG	ATTC	0.5899	164	170	+	193	4	100594349
+TNMD	CRISPRko	AAATCCAAGAAAATATGTAAATC	TTTT	0.6608	278	171	+	138	2	100585264
+TNMD	CRISPRko	TTCAATCCCTTTTTTTTCGTTGG	TTTG	0.6624	288	172	-	135	6	100599069
+TNMD	CRISPRko	CAAAATTCTGGAGATTTGTGATA	ATTC	0.6663	296	173	+	132	5	100597617
+TNMD	CRISPRko	TTTGGTTAGGCAGAAGCTTTTAA	GTTC	0.7205	379	174	+	93	2	100585243
+TNMD	CRISPRko	TTTCGTTGGCAGGAAAGTGAAGA	TTTT	0.6158	224	175	-	171	6	100599055
+TNMD	CRISPRko	AGTCCACAAATCTTAAGTGATTT	CACC	0.5519	136	176	-	215	2	100585263
+TNMD	CRISPRko	GAGAAGACCCGTCACGCCAGACA	AGTA	0.4786	2	177	+	283	6	100599147
+TNMD	CRISPRko	TGGTTCAGAAAATTCAGGAATCA	CTTC	0.6756	314	178	-	127	4	100594324
+TNMD	CRISPRko	TATTAGACCCTCCCCAGCATGCG	CTCC	0.4963	40	179	-	266	7	100599699
+TNMD	CRISPRko	TGGAGATTTGTGATAACGTGACC	ATTC	0.7062	366	180	+	106	5	100597625
+TNMD	CRISPRko	AATCAACTTACATTTAGAATGTG	TATG	0.5789	186	181	-	198	1	100585057
+TNMD	CRISPRko	CCCAGGCCTATGACATGGAGCAC	TTCT	0.6128	237	182	+	173	3	100593911
+TNMD	CRISPRko	CAGCAGAAAAGCCTATTGAAAAC	GTCC	0.5315	113	183	+	236	5	100597574
+TNMD	CRISPRko	TAAAAATTCCAAAATTCTGGAGA	TTCT	0.5474	140	184	+	223	5	100597608
+TNMD	CRISPRko	GTCGAGGCAACCGCTATTGCCGC	TGCC	0.4717	7	185	+	290	7	100599593
+TNMD	CRISPRko	CATGCTGGATGAGAGAGGTTATT	ATCC	0.6282	263	186	+	162	7	100599555
+TNMD	CRISPRko	GGAGATTTGTGATAACGTGACCA	TTCT	0.5732	187	187	+	200	5	100597626
+TNMD	CRISPRko	TTCTTTCAGACTGAAAATGGAAT	CTTC	0.6258	258	188	+	165	7	100599520
+TNMD	CRISPRko	TCAAGTGAAAGTAGAGAAGACCC	TCCC	0.6305	271	189	+	159	6	100599134
+TNMD	CRISPRko	TTTACTAGGCTACTACCCATATC	AACC	0.4847	45	190	+	276	7	100599630
+TNMD	CRISPRko	ATAACGTGACCATGTATTGGATC	TGTG	0.5436	143	191	+	227	5	100597637
+TNMD	CRISPRko	AAAAATGTTTTATCAAAACTCAG	CTTC	0.7184	406	192	+	96	4	100594313
+TNMD	CRISPRko	GAGTTACAAGACTTTGAGGAGGA	TTCT	0.5981	225	193	+	188	6	100599042
+TNMD	CRISPRko	ATACATGGTCACGTTATCACAAA	TCCA	0.4843	47	194	-	277	5	100597612
+TNMD	CRISPRko	CTGAGAGACCATGGCAAAGAATC	TGTG	0.564	190	195	+	206	1	100585030
+TNMD	CRISPRko	CCTCTGTTCTCCCAGGCCTATGA	TTTT	0.7034	386	196	+	109	3	100593901
+TNMD	CRISPRko	CTTTCCTGCCAACGAAAAAAAAG	TTCA	0.5519	180	197	+	214	6	100599080
+TNMD	CRISPRko	CCCAAAACAGGACAATTAGAGTT	CTCC	0.5126	111	198	-	254	2	100585307
+TNMD	CRISPRko	GACTGGTGTTTGGTATCCTGGCC	TGTG	0.5034	97	199	+	261	2	100585301
+TNMD	CRISPRko	TTCTTACATACCTCATCTATTTC	ATTC	0.6054	256	200	-	182	4	100594353
+TNMD	CRISPRko	AAGGTTCACAGACGCGGCGGCAA	AGTA	0.4508	1	201	-	310	7	100599590
+TNMD	CRISPRko	ACAAATCTTAAGTGATTTACATA	GTCC	0.5523	197	202	-	213	2	100585258
+TNMD	CRISPRko	AATCCCTTTTTTTTCGTTGGCAG	GTTC	0.5475	183	203	-	222	6	100599066
+TNMD	CRISPRko	AGCAGAAAAGCCTATTGAAAACC	TCCC	0.6101	272	204	+	178	5	100597575
+TNMD	CRISPRko	ACAATTCTCTGGAGGATTCTTTG	CTTC	0.5552	204	205	-	212	1	100585025
+TNMD	CRISPRko	AGGCATGATGACACGACAGATGA	TACA	0.543	171	206	-	229	7	100599658
+TNMD	CRISPRko	TAGTAAAGGTTCACAGACGCGGC	AGCC	0.4684	42	207	-	294	7	100599595
+TNMD	CRISPRko	CTCACTTGCTTGTCTGGCGTGAC	CTTC	0.6082	268	208	-	181	6	100599138
+TNMD	CRISPRko	CTCCTCCTCAAAGTCTTGTAACT	CTCC	0.5148	127	209	-	252	6	100599024
+TNMD	CRISPRko	GATTAAAGTGATTCCTGAATTTT	CTCA	0.5244	149	210	+	241	4	100594335
+TNMD	CRISPRko	AAAACTCAGATTAAAGTGATTCC	TATC	0.6127	286	211	+	174	4	100594327
+TNMD	CRISPRko	TAACTGGTGGGTGGCCCGCATGC	CTTG	0.4537	17	212	+	309	7	100599702
+TNMD	CRISPRko	TTTACTGCCGTCGAGGCAACCGC	TGTA	0.5338	168	213	+	234	7	100599584
+TNMD	CRISPRko	TCTCTCATCCAGCATGGGATCAA	AACC	0.4929	98	214	-	269	7	100599529
+TNMD	CRISPRko	AAGAAAATATGTAAATCACTTAA	ATCC	0.6615	371	215	+	136	2	100585270
+TNMD	CRISPRko	GGTAGTAGCCTAGTAAAGGTTCA	TATG	0.5919	267	216	-	191	7	100599605
+TNMD	CRISPRko	GAAGTTCTTCCTCACTTGCTTGT	ATTG	0.5558	228	217	-	211	6	100599148
+TNMD	CRISPRko	TTACATACCTCATCTATTTCCTC	CTTC	0.6111	298	218	-	176	4	100594350
+TNMD	CRISPRko	CATATCCATACTGCTACCAAGGA	TACC	0.468	61	219	+	295	7	100599647
+TNMD	CRISPRko	ACAGCAATGGAGAGAAGAAGAAG	TTCT	0.6405	351	220	+	150	3	100593941
+TNMD	CRISPRko	TCTGTCGTGTCATCATGCCTTGT	GTCA	0.4634	53	221	+	300	7	100599680
+TNMD	CRISPRko	ATATCCATACTGCTACCAAGGAG	ACCC	0.4715	71	222	+	291	7	100599648
+TNMD	CRISPRko	CTTAAAAATTCCAAAATTCTGGA	TTTT	0.6545	369	223	+	143	5	100597606
+TNMD	CRISPRko	GAAAGTGTGCTCCATGTCATAGG	TGTA	0.4984	125	224	-	265	3	100593898
+TNMD	CRISPRko	TCCTCACTTGCTTGTCTGGCGTG	TTCT	0.5382	194	225	-	231	6	100599140
+TNMD	CRISPRko	TACTGCTACCAAGGAGGACGAGT	TCCA	0.4578	43	226	+	307	7	100599655
+TNMD	CRISPRko	GTCACGCCAGACAAGCAAGTGAG	ACCC	0.4498	36	227	+	311	6	100599157
+TNMD	CRISPRko	TTCTCTCTGTTCTTTGGTTAGGC	TTTA	0.6515	372	228	+	144	2	100585231
+TNMD	CRISPRko	GTAGCAGTATGGATATGGGTAGT	CTTG	0.4673	69	229	-	296	7	100599622
+TNMD	CRISPRko	TTCTCTCCATTGCTGTAGAAAGT	CTTC	0.613	322	230	-	172	3	100593915
+TNMD	CRISPRko	ACTTAAGATTTGTGGACTGGTGT	AATC	0.5159	166	231	+	251	2	100585287
+TNMD	CRISPRko	AACCTCCTATTAGACCCTCCCCA	CTCA	0.4938	133	232	-	268	7	100599706
+TNMD	CRISPRko	CCAGCATGCGGGCCACCCACCAG	CTCC	0.432	28	233	-	323	7	100599686
+TNMD	CRISPRko	GTGCACTTCCAATGTTTCATCAG	AGTC	0.4273	22	234	-	327	3	100594003
+TNMD	CRISPRko	CTCAAGTGAAAGTAGAGAAGACC	GTCC	0.4728	102	235	+	288	6	100599133
+TNMD	CRISPRko	CAATTCTCTGGAGGATTCTTTGC	TTCA	0.4956	150	236	-	267	1	100585024
+TNMD	CRISPRko	TCTTACATACCTCATCTATTTCC	TTCT	0.5335	216	237	-	235	4	100594352
+TNMD	CRISPRko	AAGGAGGACGAGTCATCTGTCGT	TACC	0.5431	231	238	+	228	7	100599665
+TNMD	CRISPRko	ACAACTTTCTTTGAACAGTCAGT	TACC	0.5202	195	239	+	246	5	100597540
+TNMD	CRISPRko	AACAAAATGAACAGTGGGTGGTC	ATTG	0.5881	301	240	+	194	6	100599109
+TNMD	CRISPRko	GTTCAATCCCTTTTTTTTCGTTG	TTTT	0.5933	310	241	-	190	6	100599070
+TNMD	CRISPRko	TTTCAGACTGAAAATGGAATAGA	CTTC	0.64	389	242	+	151	7	100599523
+TNMD	CRISPRko	ATCCCTTTTTTTTCGTTGGCAGG	TTCA	0.549	252	243	-	220	6	100599065
+TNMD	CRISPRko	TGAATATTTCAGTTCTGGTCACA	CTTC	0.6259	365	244	-	164	3	100593965
+TNMD	CRISPRko	ACAATAACCTCTCTCATCCAGCA	TACA	0.5068	178	245	-	258	7	100599538
+TNMD	CRISPRko	CAACAATAACCTCTCTCATCCAG	AATA	0.4655	100	246	-	299	7	100599540
+TNMD	CRISPRko	TGTTTTGGGGGAGCAAGCACTTC	GTCC	0.4918	156	247	+	271	2	100585340
+TNMD	CRISPRko	TACCTGATATTAGAGTGGGATTG	GTCA	0.4281	49	248	-	326	5	100597640
+TNMD	CRISPRko	ACTTACATTTAGAATGTGACAGT	ATCA	0.5162	201	249	-	250	1	100585052
+TNMD	CRISPRko	ATTTTGTTCAATCCCTTTTTTTT	GTTC	0.6357	394	250	-	155	6	100599075
+TNMD	CRISPRko	ACTTACGTTTTTAAAGTCGTGCA	TCCA	0.4547	92	251	-	308	3	100594021
+TNMD	CRISPRko	TTGTAACTGGTGGGTGGCCCGCA	TGCC	0.5095	199	252	+	256	7	100599699
+TNMD	CRISPRko	ACAAATCTCCAGAATTTTGGAAT	TATC	0.5906	327	253	-	192	5	100597594
+TNMD	CRISPRko	ACTTTAATCTGAGTTTTGATAAA	AATC	0.5176	218	254	-	248	4	100594302
+TNMD	CRISPRko	ATTTCCGCTTCTGAATATTTCAG	TGCC	0.5307	241	255	-	237	3	100593976
+TNMD	CRISPRko	CTCTGTTCTTTGGTTAGGCAGAA	TTCT	0.4821	158	256	+	279	2	100585235
+TNMD	CRISPRko	TTGAACAGTCAGTGATTTGGGTC	TTCT	0.5017	192	257	+	263	5	100597550
+TNMD	CRISPRko	TAAGCTACTTTCTTCTTTCAGAA	ACCC	0.4624	122	258	+	301	5	100597504
+TNMD	CRISPRko	GAATTTGATCCCATGCTGGATGA	AATA	0.4167	60	259	+	335	7	100599544
+TNMD	CRISPRko	TTTCAGAATGAAGAAATTACCAC	CTTC	0.6254	400	260	+	166	5	100597519
+TNMD	CRISPRko	CTGACTGTTCAAAGAAAGTTGTG	ATCA	0.4623	131	261	-	302	5	100597520
+TNMD	CRISPRko	TAAATCACTTAAGATTTGTGGAC	TATG	0.5563	320	262	+	210	2	100585281
+TNMD	CRISPRko	TAAACATAACTCACATAGTCATT	TACA	0.5712	339	263	-	202	6	100599176
+TNMD	CRISPRko	AGACAAGCAAGTGAGGAAGAACT	CGCC	0.562	329	264	+	207	6	100599165
+TNMD	CRISPRko	TCATGCCTTGTAACTGGTGGGTG	GTCA	0.4318	96	265	+	324	7	100599692
+TNMD	CRISPRko	GTGTCATCATGCCTTGTAACTGG	TGTC	0.4251	87	266	+	329	7	100599686
+TNMD	CRISPRko	GAGGCAACCGCTATTGCCGCCGC	CGTC	0.3881	29	267	+	359	7	100599596
+TNMD	CRISPRko	TGGGTCTTCAAAAATGTTTTATC	TTCG	0.566	341	268	+	204	4	100594304
+TNMD	CRISPRko	TCCTTGGTAGCAGTATGGATATG	GTCC	0.4143	76	269	-	337	7	100599628
+TNMD	CRISPRko	CAGCATGCGGGCCACCCACCAGT	TCCC	0.4147	82	270	-	336	7	100599685
+TNMD	CRISPRko	TATTGAAAACCGAGATTTTCTTA	AGCC	0.547	306	271	+	224	5	100597587
+TNMD	CRISPRko	GCATGGGATCAAATTCTATTCCA	TCCA	0.461	145	272	-	305	7	100599518
+TNMD	CRISPRko	TTCTTCTTACATACCTCATCTAT	ATTA	0.5378	294	273	-	232	4	100594356
+TNMD	CRISPRko	ACGCCAGACAAGCAAGTGAGGAA	CGTC	0.3553	13	274	+	373	6	100599160
+TNMD	CRISPRko	AGGCCTATGACATGGAGCACACT	TCCC	0.4863	213	275	+	275	3	100593914
+TNMD	CRISPRko	AATCACTTAAGATTTGTGGACTG	TGTA	0.4901	220	276	+	272	2	100585283
+TNMD	CRISPRko	TGGATATGGGTAGTAGCCTAGTA	AGTA	0.4001	67	277	-	350	7	100599613
+TNMD	CRISPRko	GCGTCTGTGAACCTTTACTAGGC	CGCC	0.3718	32	278	+	368	7	100599617
+TNMD	CRISPRko	CTTTTTTTTCGTTGGCAGGAAAG	ATCC	0.4324	130	279	-	319	6	100599061
+TNMD	CRISPRko	ATGTGTATTTACTTACTTTTTTG	TATG	0.578	370	280	-	199	2	100585358
+TNMD	CRISPRko	GCCTAGTAAAGGTTCACAGACGC	AGTA	0.3926	63	281	-	354	7	100599598
+TNMD	CRISPRko	AGCATGCGGGCCACCCACCAGTT	CCCC	0.4051	83	282	-	345	7	100599684
+TNMD	CRISPRko	GCAGGAAAGTGAAGATCTTCTCC	GTTG	0.4747	202	283	-	286	6	100599047
+TNMD	CRISPRko	TTTTGTCTGTTTTATAGGGATAC	GTTG	0.5876	384	284	+	195	4	100594265
+TNMD	CRISPRko	CTGTTTTGGGGGAGCAAGCACTT	TGTC	0.4883	233	285	+	273	2	100585339
+TNMD	CRISPRko	TTGGTTAGGCAGAAGCTTTTAAA	TTCT	0.5612	364	286	+	208	2	100585244
+TNMD	CRISPRko	GCTATTGCCGCCGCGTCTGTGAA	AACC	0.3278	19	287	+	382	7	100599605
+TNMD	CRISPRko	CCAAAACAGGACAATTAGAGTTA	TCCC	0.5303	307	288	-	238	2	100585306
+TNMD	CRISPRko	TCGGTTTTCAATAGGCTTTTCTG	AATC	0.4119	106	289	-	339	5	100597558
+TNMD	CRISPRko	CTGTGTGCTGAGAGACCATGGCA	AGTA	0.4813	226	290	+	281	1	100585023
+TNMD	CRISPRko	CTTCCAATGTTTCATCAGTGCCA	TGCA	0.4383	161	291	-	315	3	100593998
+TNMD	CRISPRko	ACTGACTGTTCAAAGAAAGTTGT	AATC	0.527	315	292	-	239	5	100597521
+TNMD	CRISPRko	AGAAAATATGTAAATCACTTAAG	TCCA	0.5517	361	293	+	216	2	100585271
+TNMD	CRISPRko	AAAACCGAGATTTTCTTAAAAAT	ATTG	0.5505	358	294	+	218	5	100597592
+TNMD	CRISPRko	CTTTAATCTGAGTTTTGATAAAA	ATCA	0.505	274	295	-	260	4	100594301
+TNMD	CRISPRko	CTTAAGATTTGTGGACTGGTGTT	ATCA	0.4601	182	296	+	306	2	100585288
+TNMD	CRISPRko	TTCTTCTCTCCATTGCTGTAGAA	CTTC	0.4866	247	297	-	274	3	100593918
+TNMD	CRISPRko	TGCTGAGAGACCATGGCAAAGAA	TGTG	0.4113	115	298	+	340	1	100585028
+TNMD	CRISPRko	CAAGAAAATATGTAAATCACTTA	AATC	0.5664	390	299	+	203	2	100585269
+TNMD	CRISPRko	ACAGTCTTCACAATTCTCTGGAG	TGTG	0.4798	234	300	-	282	1	100585034
+TNMD	CRISPRko	AAATCACTGACTGTTCAAAGAAA	ACCC	0.5188	305	301	-	247	5	100597526
+TNMD	CRISPRko	TTTTGTTCAATCCCTTTTTTTTC	TTCA	0.5722	399	302	-	201	6	100599074
+TNMD	CRISPRko	ATTTCCATGTAAATCTTCTTCTT	ATCA	0.4242	142	303	-	331	3	100593936
+TNMD	CRISPRko	GCGGTTGCCTCGACGGCAGTAAA	AATA	0.382	79	304	-	363	7	100599565
+TNMD	CRISPRko	TCCTATTAGACCCTCCCCAGCAT	AACC	0.3834	90	305	-	361	7	100599702
+TNMD	CRISPRko	CTACTTTCACTTGAGGGACCACC	TTCT	0.3805	86	306	-	365	6	100599106
+TNMD	CRISPRko	TGGAAATTGATCCTGTGACCAGA	TACA	0.462	212	307	+	303	3	100593971
+TNMD	CRISPRko	TGTTTTATAGGGATACACTGGCA	TGTC	0.521	332	308	+	244	4	100594272
+TNMD	CRISPRko	GAATATTTCAGTTCTGGTCACAG	TTCT	0.3536	68	309	-	376	3	100593964
+TNMD	CRISPRko	AATCTTCTTCTTCTCTCCATTGC	TGTA	0.4768	250	310	-	285	3	100593925
+TNMD	CRISPRko	CAGGATCAATTTCCATGTAAATC	GTCA	0.5134	319	311	-	253	3	100593944
+TNMD	CRISPRko	ATCATGCCTTGTAACTGGTGGGT	TGTC	0.4772	260	312	+	284	7	100599691
+TNMD	CRISPRko	ACCAGTTACAAGGCATGATGACA	ACCC	0.2854	44	313	-	395	7	100599668
+TNMD	CRISPRko	CTTGCTTGTCTGGCGTGACGGGT	CTCA	0.3096	55	314	-	390	6	100599134
+TNMD	CRISPRko	CGCCAGACAAGCAAGTGAGGAAG	GTCA	0.1794	27	315	+	405	6	100599161
+TNMD	CRISPRko	TTCTTCTTTCAGACTGAAAATGG	CTTC	0.5118	330	316	+	255	7	100599517
+TNMD	CRISPRko	TCCTGTTTTGGGGGAGCAAGCAC	ATTG	0.5034	317	317	+	262	2	100585337
+TNMD	CRISPRko	CTGTAGAAAGTGTGCTCCATGTC	ATTG	0.423	181	318	-	332	3	100593903
+TNMD	CRISPRko	CACCAGTTACAAGGCATGATGAC	CACC	0.2579	46	319	-	400	7	100599669
+TNMD	CRISPRko	AAAATGTTTTATCAAAACTCAGA	TTCA	0.5485	405	320	+	221	4	100594314
+TNMD	CRISPRko	TGTAAATCACTTAAGATTTGTGG	AATA	0.4816	292	321	+	280	2	100585279
+TNMD	CRISPRko	TCTTTCAGAATGAAGAAATTACC	TTCT	0.5469	404	322	+	225	5	100597517
+TNMD	CRISPRko	AACCTTTACTAGGCTACTACCCA	TGTG	0.3351	99	323	+	380	7	100599626
+TNMD	CRISPRko	TTGTTTTGTCTGTTTTATAGGGA	ATTG	0.546	407	324	+	226	4	100594262
+TNMD	CRISPRko	TTTTCGTTGGCAGGAAAGTGAAG	TTTT	0.3801	129	325	-	366	6	100599056
+TNMD	CRISPRko	CATGGTCACGTTATCACAAATCT	AATA	0.3929	159	326	-	353	5	100597609
+TNMD	CRISPRko	TTGGTAGCAGTATGGATATGGGT	CTCC	0.4102	184	327	-	341	7	100599625
+TNMD	CRISPRko	CATAGTCATTTATTGGAAGTTCT	CTCA	0.5053	348	328	-	259	6	100599163
+TNMD	CRISPRko	AGAATTTTGGAATTTTTAAGAAA	CTCC	0.5227	382	329	-	243	5	100597584
+TNMD	CRISPRko	AAATTCTGGAGATTTGTGATAAC	TCCA	0.4418	246	330	+	312	5	100597619
+TNMD	CRISPRko	TTGTATTTACTGCCGTCGAGGCA	ATTG	0.5229	388	331	+	242	7	100599579
+TNMD	CRISPRko	TCACAAATCTCCAGAATTTTGGA	GTTA	0.499	344	332	-	264	5	100597596
+TNMD	CRISPRko	CTGGCATCTACTTCGTGGGTCTT	TACA	0.5258	392	333	+	240	4	100594289
+TNMD	CRISPRko	GGCCAGAAGTGCTTGCTCCCCCA	CTCC	0.301	89	334	-	393	2	100585326
+TNMD	CRISPRko	GAGAATTGTGAAGACTGTCACAT	TCCA	0.3218	108	335	+	384	1	100585058
+TNMD	CRISPRko	TCCTCAAAGTCTTGTAACTCAGA	CTCC	0.4008	179	336	-	349	6	100599020
+TNMD	CRISPRko	GTGATTTGGGTCCCAGCAGAAAA	GTCA	0.4661	282	337	+	298	5	100597561
+TNMD	CRISPRko	CAAAACAGGACAATTAGAGTTAG	CCCC	0.484	323	338	-	278	2	100585305
+TNMD	CRISPRko	TTTACTTACTTTTTTGGGTACCT	TGTA	0.5169	383	339	-	249	2	100585351
+TNMD	CRISPRko	TATTTTCTTGGATTTAAAAGCTT	TACA	0.5076	368	340	-	257	2	100585237
+TNMD	CRISPRko	AGACTTTGAGGAGGAGGGAGAAG	TACA	0.4612	277	341	+	304	6	100599050
+TNMD	CRISPRko	AACTTACATTTAGAATGTGACAG	AATC	0.4726	308	342	-	289	1	100585053
+TNMD	CRISPRko	ACATGGAGCACACTTTCTACAGC	TATG	0.4294	239	343	+	325	3	100593923
+TNMD	CRISPRko	TCCCCAGCATGCGGGCCACCCAC	ACCC	0.4255	236	344	-	328	7	100599689
+TNMD	CRISPRko	AAACTCAGATTAAAGTGATTCCT	ATCA	0.467	299	345	+	297	4	100594328
+TNMD	CRISPRko	ATCTGTCGTGTCATCATGCCTTG	AGTC	0.4089	210	346	+	342	7	100599679
+TNMD	CRISPRko	TATTTACTTACTTTTTTGGGTAC	TGTG	0.5202	408	347	-	245	2	100585353
+TNMD	CRISPRko	TTTAGAATGTGACAGTCTTCACA	TACA	0.4342	269	348	-	318	1	100585045
+TNMD	CRISPRko	AGAGAATTGTGAAGACTGTCACA	CTCC	0.4693	325	349	+	293	1	100585057
+TNMD	CRISPRko	CACAAATCTTAAGTGATTTACAT	AGTC	0.315	138	350	-	388	2	100585259
+TNMD	CRISPRko	CTCCTTGGTAGCAGTATGGATAT	CGTC	0.4077	230	351	-	343	7	100599629
+TNMD	CRISPRko	CTCCATTGCTGTAGAAAGTGTGC	TTCT	0.3448	160	352	-	378	3	100593911
+TNMD	CRISPRko	TCAAAGTCTTGTAACTCAGAAAC	CTCC	0.4348	284	353	-	317	6	100599017
+TNMD	CRISPRko	AACTTACGTTTTTAAAGTCGTGC	ATCC	0.4387	297	354	-	314	3	100594022
+TNMD	CRISPRko	TGAATCAACTTACATTTAGAATG	AATA	0.4928	387	355	-	270	1	100585059
+TNMD	CRISPRko	ATGTTTCATCAGTGCCATTTCCG	TCCA	0.4364	300	356	-	316	3	100593992
+TNMD	CRISPRko	ACCTCTCTCATCCAGCATGGGAT	AATA	0.2824	139	357	-	397	7	100599532
+TNMD	CRISPRko	TCATCTATTTCCTCTTCTGGTTC	TACC	0.4737	360	358	-	287	4	100594341
+TNMD	CRISPRko	ACCAGAACTGAAATATTCAGAAG	TGTG	0.3731	208	359	+	367	3	100593988
+TNMD	CRISPRko	TCTCTCCATTGCTGTAGAAAGTG	TTCT	0.3811	217	360	-	364	3	100593914
+TNMD	CRISPRko	TCCAGAATTTTGGAATTTTTAAG	AATC	0.4037	254	361	-	348	5	100597587
+TNMD	CRISPRko	GTGCCATTTCCGCTTCTGAATAT	ATCA	0.3137	174	362	-	389	3	100593981
+TNMD	CRISPRko	CGTTATCACAAATCTCCAGAATT	GTCA	0.4245	293	363	-	330	5	100597601
+TNMD	CRISPRko	TTCCTCACTTGCTTGTCTGGCGT	GTTC	0.422	290	364	-	334	6	100599141
+TNMD	CRISPRko	CCCTCCTCCTCAAAGTCTTGTAA	TTCT	0.3563	214	365	-	372	6	100599026
+TNMD	CRISPRko	TTAAGTGATTTACATATTTTCTT	AATC	0.4699	377	366	-	292	2	100585251
+TNMD	CRISPRko	AATACAACAATAACCTCTCTCAT	AGTA	0.3633	238	367	-	370	7	100599544
+TNMD	CRISPRko	AACGAAAAAAAAGGGATTGAACA	TGCC	0.4321	336	368	+	321	6	100599090
+TNMD	CRISPRko	AGTGATTTGGGTCCCAGCAGAAA	AGTC	0.3163	206	369	+	386	5	100597560
+TNMD	CRISPRko	AAAACAGGACAATTAGAGTTAGG	CCCC	0.4321	335	370	-	322	2	100585304
+TNMD	CRISPRko	TACATACCTCATCTATTTCCTCT	TTCT	0.3858	266	371	-	360	4	100594349
+TNMD	CRISPRko	CAAATCTTAAGTGATTTACATAT	TCCA	0.4138	311	372	-	338	2	100585257
+TNMD	CRISPRko	TTTATTATCTTAGTTTCTGAGTT	TGCA	0.4401	362	373	+	313	6	100599024
+TNMD	CRISPRko	AGTTACAAGGCATGATGACACGA	CACC	0.3832	270	374	-	362	7	100599665
+TNMD	CRISPRko	AGTGAAAGTAGAGAAGACCCGTC	CTCA	0.405	309	375	+	346	6	100599137
+TNMD	CRISPRko	GGCTTTTCTGCTGGGACCCAAAT	AATA	0.238	198	376	-	402	5	100597545
+TNMD	CRISPRko	TCCTCCTCAAAGTCTTGTAACTC	TCCC	0.3919	295	377	-	355	6	100599023
+TNMD	CRISPRko	TTGCTGTAGAAAGTGTGCTCCAT	TCCA	0.3062	227	378	-	391	3	100593906
+TNMD	CRISPRko	TGCCTTGTAACTGGTGGGTGGCC	ATCA	0.2226	211	379	+	404	7	100599695
+TNMD	CRISPRko	TGAGTTTTGATAAAACATTTTTG	AATC	0.4225	353	380	-	333	4	100594293
+TNMD	CRISPRko	TTCAGAAGCGGAAATGGCACTGA	AATA	0.3681	283	381	+	369	3	100594003
+TNMD	CRISPRko	ACAATTATTCTTCTTACATACCT	CACC	0.3317	262	382	-	381	4	100594363
+TNMD	CRISPRko	GGTTCAGAAAATTCAGGAATCAC	TTCT	0.4324	385	383	-	320	4	100594323
+TNMD	CRISPRko	CTCCAGAGAATTGTGAAGACTGT	AATC	0.3982	324	384	+	351	1	100585053
+TNMD	CRISPRko	ATTTATTGGAAGTTCTTCCTCAC	AGTC	0.4067	340	385	-	344	6	100599156
+TNMD	CRISPRko	TACCTCATCTATTTCCTCTTCTG	TACA	0.3219	264	386	-	383	4	100594345
+TNMD	CRISPRko	TGTAAATCTTCTTCTTCTCTCCA	TCCA	0.397	328	387	-	352	3	100593929
+TNMD	CRISPRko	TTTCAGTTCTGGTCACAGGATCA	AATA	0.2629	244	388	-	399	3	100593959
+TNMD	CRISPRko	TTCACAATTCTCTGGAGGATTCT	AGTC	0.1498	242	389	-	408	1	100585028
+TNMD	CRISPRko	CAAATCTCCAGAATTTTGGAATT	ATCA	0.3906	347	390	-	357	5	100597593
+TNMD	CRISPRko	TTTTTTTTCGTTGGCAGGAAAGT	TCCC	0.4041	373	391	-	347	6	100599060
+TNMD	CRISPRko	AGCATGGGATCAAATTCTATTCC	ATCC	0.3539	318	392	-	375	7	100599519
+TNMD	CRISPRko	ACTGTTCATTTTGTTCAATCCCT	ACCC	0.2933	287	393	-	394	6	100599082
+TNMD	CRISPRko	GAGATTTTCTTAAAAATTCCAAA	AACC	0.3903	378	394	+	358	5	100597598
+TNMD	CRISPRko	TCCAGCATGGGATCAAATTCTAT	CTCA	0.3189	334	395	-	385	7	100599522
+TNMD	CRISPRko	GCAATGGAGAGAAGAAGAAGATT	TACA	0.3151	331	396	+	387	3	100593944
+TNMD	CRISPRko	TCTTTCAGACTGAAAATGGAATA	TTCT	0.3917	396	397	+	356	7	100599521
+TNMD	CRISPRko	TTTTCCTCTGTTCTCCCAGGCCT	ATTG	0.3543	367	398	+	374	3	100593897
+TNMD	CRISPRko	TTCAGAATGAAGAAATTACCACA	TTCT	0.3028	349	399	+	392	5	100597520
+TNMD	CRISPRko	TTCAGACTGAAAATGGAATAGAA	TTCT	0.3573	393	400	+	371	7	100599524
+TNMD	CRISPRko	GAATTTTGGAATTTTTAAGAAAA	TCCA	0.3467	381	401	-	377	5	100597583
+TNMD	CRISPRko	CACTGTTCATTTTGTTCAATCCC	CACC	0.1618	343	402	-	407	6	100599083
+TNMD	CRISPRko	TTCTTCTTCTCTCCATTGCTGTA	AATC	0.2691	363	403	-	398	3	100593921
+TNMD	CRISPRko	TCTTCTCTCCATTGCTGTAGAAA	TTCT	0.3423	401	404	-	379	3	100593917
+TNMD	CRISPRko	TCTATTTCCTCTTCTGGTTCAGA	CTCA	0.2534	359	405	-	401	4	100594338
+TNMD	CRISPRko	TTTATTGGAAGTTCTTCCTCACT	GTCA	0.2343	375	406	-	403	6	100599155
+TNMD	CRISPRko	TCTTCTTTCAGACTGAAAATGGA	TTCT	0.2853	402	407	+	396	7	100599518
+TNMD	CRISPRko	CCTTTTTTTTCGTTGGCAGGAAA	AATC	0.1761	391	408	-	406	6	100599062
+DPM1	CRISPRko	CCTACCTACAACGAGCGCGAGAA	TTTA	0.9438	5	1	-	8	1	50958410
+DPM1	CRISPRko	GTGGAATCAACTATGAAATTATA	TTTA	0.9859	42	2	-	2	2	50955266
+DPM1	CRISPRko	ACCAGCAGCCACACGATGAGCGG	TTTC	0.8731	57	3	+	18	1	50958402
+DPM1	CRISPRko	CACGACTTACCTAGTCCCAACTT	ATTA	0.8147	20	4	+	37	3	50948640
+DPM1	CRISPRko	GAGATTACACGACTTACCTAGTC	GTTA	0.7878	22	5	+	48	3	50948633
+DPM1	CRISPRko	TCGCGCTCGTTGTAGGTAGGTAA	GTTC	0.7506	1	6	+	62	1	50958432
+DPM1	CRISPRko	TGTGGCATGTTTCATTCCATGAA	TTCC	0.851	76	7	+	25	4	50945910
+DPM1	CRISPRko	TTCTGTCGTGGACTGCGCACTTC	TTTG	0.7479	18	8	+	66	1	50958471
+DPM1	CRISPRko	TTAGTGGAATCAACTATGAAATT	TTTT	0.8108	83	9	-	38	2	50955269
+DPM1	CRISPRko	CCAGCAGCCACACGATGAGCGGC	TTCA	0.7473	27	10	+	67	1	50958403
+DPM1	CRISPRko	GGTGCTTTTACCTACCTACAACG	ATTC	0.776	60	11	-	51	1	50958420
+DPM1	CRISPRko	ATTCCATGAATATATGCAGTTCC	TTTC	0.9711	166	12	+	4	4	50945923
+DPM1	CRISPRko	GGAACTGCATATATTCATGGAAT	TTTA	0.9085	158	13	-	10	4	50945904
+DPM1	CRISPRko	TAACTTACAATTCTGTCTGACCC	CTTA	0.7515	66	14	+	61	2	50955199
+DPM1	CRISPRko	TAATCATAGATGATGGAAGCCCA	ATTA	0.7705	82	15	-	54	2	50955245
+DPM1	CRISPRko	ACCATAAACACGATCCACAAATG	ATTC	0.7781	99	16	+	50	9	50935225
+DPM1	CRISPRko	TTATTCCTACAGCTTCTAAGACC	TTTC	0.8659	161	17	-	20	3	50948654
+DPM1	CRISPRko	TGTCTGACCCATAGATCTTCTCC	ATTC	0.719	43	18	+	82	2	50955212
+DPM1	CRISPRko	AATATCATTTGTGGATCGTGTTT	TTCC	0.759	96	19	-	57	9	50935211
+DPM1	CRISPRko	CTGTGGCATGTTTCATTCCATGA	TTTC	0.8629	168	20	+	21	4	50945909
+DPM1	CRISPRko	ATGGAATGAAACATGCCACAGGA	ATTC	0.8571	167	21	-	24	4	50945888
+DPM1	CRISPRko	GCCATGGCCTCCTTGGAAGTCAG	TTCC	0.728	58	22	-	79	1	50958506
+DPM1	CRISPRko	GATATTGTCTCTGGAACTCGCTA	TTTT	0.7497	91	23	-	64	6	50942084
+DPM1	CRISPRko	AAATTTCAGCCAAAATTTATTCC	TTTC	0.8924	192	24	-	14	5	50945751
+DPM1	CRISPRko	TACAGCTTCTAAGACCACGAGAG	TTCC	0.7083	23	25	-	99	3	50948647
+DPM1	CRISPRko	GAAATAGTACCTACCTAATAAAT	ATTA	0.8479	172	26	+	27	5	50945744
+DPM1	CRISPRko	TAGTGGAATCAACTATGAAATTA	TTTT	0.7744	122	27	-	52	2	50955268
+DPM1	CRISPRko	CAATTCTGTCTGACCCATAGATC	CTTA	0.7177	62	28	+	84	2	50955206
+DPM1	CRISPRko	AATCCCAGCCATATACACCTCCA	TTCA	0.7394	92	29	+	71	6	50942071
+DPM1	CRISPRko	CCTAGTCCCAACTTTTTCTCTCG	CTTA	0.7125	48	30	+	93	3	50948649
+DPM1	CRISPRko	CTACAGCTTCTAAGACCACGAGA	ATTC	0.7018	33	31	-	103	3	50948648
+DPM1	CRISPRko	CAGCTCCCGCCGAGACCTGCGAG	CTTC	0.7163	72	32	+	89	1	50958494
+DPM1	CRISPRko	GGGCAAGACAGTTGAATTATACT	GTTC	0.7174	102	33	-	85	8	50936159
+DPM1	CRISPRko	CTTTTCAAATCCCAGCCATATAC	TTTT	0.7549	154	34	+	59	6	50942064
+DPM1	CRISPRko	TTCCTACAGCTTCTAAGACCACG	CTTA	0.7098	78	35	-	97	3	50948651
+DPM1	CRISPRko	TAAACTAGGTTCCAATATCATTT	TTTT	0.7433	135	36	-	69	9	50935224
+DPM1	CRISPRko	TGGATGCTGATCTCTCACACCAT	ATTA	0.7332	125	37	-	74	4	50945849
+DPM1	CRISPRko	TAACTCAGATCTTGCTGAGACCA	TTTT	0.731	126	38	-	75	7	50940896
+DPM1	CRISPRko	TACCGAAAAGAAGTTCTAGAGAA	ATTA	0.7201	118	39	-	81	8	50936238
+DPM1	CRISPRko	GGTAGGTAAAAGCACCGAATATT	TGTA	0.6592	21	40	+	130	1	50958446
+DPM1	CRISPRko	AATCAGATGCTCCTGGTCTCAGC	GTTA	0.7427	145	41	+	70	7	50940902
+DPM1	CRISPRko	ACCTACCTACAACGAGCGCGAGA	TTTT	0.6408	2	42	-	143	1	50958411
+DPM1	CRISPRko	AGCCAAAATTTATTCCTGAATTT	TTTC	0.8864	259	43	-	15	5	50945744
+DPM1	CRISPRko	GTGCTTTTACCTACCTACAACGA	TTCG	0.6643	40	44	-	125	1	50958419
+DPM1	CRISPRko	TTTGTAGCGAGTTCCAGAGACAA	TTCC	0.7248	132	45	+	80	6	50942099
+DPM1	CRISPRko	CATGGTGTGAGAGATCAGCATCC	CTTA	0.7916	204	46	+	47	4	50945867
+DPM1	CRISPRko	AACTGTCTTGCCCGAACAATCAT	ATTC	0.6944	90	47	+	107	8	50936188
+DPM1	CRISPRko	TAAGACCACGAGAGAAAAAGTTG	CTTC	0.8261	234	48	-	35	3	50948638
+DPM1	CRISPRko	GAGAAGATCTATGGGTCAGACAG	GTTG	0.6368	19	49	-	146	2	50955192
+DPM1	CRISPRko	CAGATGGAGATGATTGTTCGGGC	CTTC	0.7134	130	50	-	91	8	50936178
+DPM1	CRISPRko	ACTGTACCTGAAACTTCCTGTTA	TTTC	1.03	312	51	+	1	7	50940879
+DPM1	CRISPRko	ATTTGGCAGCCGTGGGGCCAATT	TTTT	0.7089	119	52	-	98	7	50940922
+DPM1	CRISPRko	AGAGACAATATCAAAATTACCCT	TTCC	0.8721	277	53	+	19	6	50942114
+DPM1	CRISPRko	CGCCATGGCCTCCTTGGAAGTCA	GTTC	0.6474	36	54	-	140	1	50958507
+DPM1	CRISPRko	GTCGTGGACTGCGCACTTCCAGC	TTCT	0.6237	7	55	+	156	1	50958475
+DPM1	CRISPRko	GTCTGACCCATAGATCTTCTCCA	TTCT	0.6348	24	56	+	148	2	50955213
+DPM1	CRISPRko	AGCAACATCCCTTGTTCCATCTG	GTTC	0.6399	35	57	+	144	2	50955243
+DPM1	CRISPRko	GCATCAGTTGCATACCTCGCCAA	TTTA	0.9234	306	58	+	9	8	50936154
+DPM1	CRISPRko	TCTAGAACTTCTTTTCGGTATAA	TTTC	0.9754	319	59	+	3	8	50936260
+DPM1	CRISPRko	ACTCAGATCTTGCTGAGACCAGG	TTTA	0.9466	315	60	-	6	7	50940894
+DPM1	CRISPRko	TAGGAACTGCATATATTCATGGA	TTTT	0.7537	208	61	-	60	4	50945906
+DPM1	CRISPRko	TGTAGTAGCAAAAAGAGTCAATA	TTTA	0.9643	320	62	+	5	9	50935156
+DPM1	CRISPRko	AAATCCCAGCCATATACACCTCC	TTTC	0.9457	316	63	+	7	6	50942070
+DPM1	CRISPRko	AGCTCCCGCCGAGACCTGCGAGG	TTCC	0.6322	28	64	+	151	1	50958495
+DPM1	CRISPRko	GGCATCTGGCTCAGTTCCGCCAT	TTCC	0.6589	68	65	-	131	1	50958524
+DPM1	CRISPRko	TCCCAACTTGGATTCACCATAAA	TTCC	0.6856	103	66	+	115	9	50935210
+DPM1	CRISPRko	TAAAGGCTACGTCTTCCAGATGG	TTTC	0.9062	311	67	-	11	8	50936194
+DPM1	CRISPRko	ATTTTTAGGAACTGCATATATTC	TTTC	0.8592	287	68	-	23	4	50945911
+DPM1	CRISPRko	TGGAATGAAACATGCCACAGGAA	TTCA	0.6542	69	69	-	134	4	50945887
+DPM1	CRISPRko	CGCGCTCGTTGTAGGTAGGTAAA	TTCT	0.6119	6	70	+	166	1	50958433
+DPM1	CRISPRko	TGGTGAATCCAAGTTGGGAGGAA	TTTA	0.9028	314	71	-	13	9	50935187
+DPM1	CRISPRko	ATCATAGATGATGGAAGCCCAGA	TATA	0.6291	34	72	-	153	2	50955243
+DPM1	CRISPRko	TACAGGAAGCAAAAGGAGGGTAA	ATTC	0.7299	191	73	-	76	6	50942111
+DPM1	CRISPRko	TTTAGTGGAATCAACTATGAAAT	TTTT	0.7581	227	74	-	58	2	50955270
+DPM1	CRISPRko	TGTCGTGGACTGCGCACTTCCAG	GTTC	0.7106	155	75	+	95	1	50958474
+DPM1	CRISPRko	TAACACATGTACCTACCTGATTA	GTTG	0.6659	101	76	+	124	6	50942036
+DPM1	CRISPRko	AGGAACTGCATATATTCATGGAA	TTTT	0.7605	237	77	-	56	4	50945905
+DPM1	CRISPRko	ATTTTTTTAGTGGAATCAACTAT	TTTA	0.8407	289	78	-	30	2	50955275
+DPM1	CRISPRko	CAATATCATTTGTGGATCGTGTT	GTTC	0.694	131	79	-	110	9	50935212
+DPM1	CRISPRko	TTACAGATTATACCGAAAAGAAG	TTTC	0.9052	327	80	-	12	8	50936248
+DPM1	CRISPRko	ACTGTCTTGCCCGAACAATCATC	TTCA	0.6595	94	81	+	129	8	50936189
+DPM1	CRISPRko	CTGTACCTGAAACTTCCTGTTAA	TTCA	0.717	178	82	+	87	7	50940880
+DPM1	CRISPRko	AACTAGGTTCCAATATCATTTGT	TTTA	0.8848	322	83	-	16	9	50935222
+DPM1	CRISPRko	CTAGAACTTCTTTTCGGTATAAT	TTCT	0.6809	121	84	+	117	8	50936261
+DPM1	CRISPRko	CAGATTATACCGAAAAGAAGTTC	CTTA	0.7473	220	85	-	68	8	50936245
+DPM1	CRISPRko	TCGCCAATAGTATAATTCAACTG	TACC	0.6584	93	86	+	132	8	50936170
+DPM1	CRISPRko	TACCTACAACGAGCGCGAGAACC	TACC	0.6004	4	87	-	177	1	50958408
+DPM1	CRISPRko	GCAACATCCCTTGTTCCATCTGG	TTCA	0.7127	175	88	+	92	2	50955244
+DPM1	CRISPRko	TTTTCAAATCCCAGCCATATACA	TTTC	0.8788	326	89	+	17	6	50942065
+DPM1	CRISPRko	ATAGAAAAATGTGTTTCTAAAGG	ATTA	0.8323	297	90	-	32	8	50936211
+DPM1	CRISPRko	CTTTGTAGCGAGTTCCAGAGACA	TTTC	0.8489	309	91	+	26	6	50942098
+DPM1	CRISPRko	CAGAGACAATATCAAAATTACCC	GTTC	0.7627	251	92	+	55	6	50942113
+DPM1	CRISPRko	GATTCACCATAAACACGATCCAC	CTTG	0.6511	89	93	+	137	9	50935220
+DPM1	CRISPRko	CTATTAATTTCTCTAGAACTTCT	TTTT	0.7148	183	94	+	90	8	50936249
+DPM1	CRISPRko	GCCAAAATTTATTCCTGAATTTA	TTCA	0.7066	164	95	-	101	5	50945743
+DPM1	CRISPRko	GGAGGAAATGAAATAGTATCTTT	GTTG	0.75	241	96	-	63	9	50935171
+DPM1	CRISPRko	AGTGGAATCAACTATGAAATTAT	TTTT	0.829	301	97	-	33	2	50955267
+DPM1	CRISPRko	AAGAAAGATACTATTTCATTTCC	TTTC	0.8622	325	98	+	22	9	50935187
+DPM1	CRISPRko	TTAAACTAGGTTCCAATATCATT	TTTT	0.7358	224	99	-	73	9	50935225
+DPM1	CRISPRko	CCCGAACAATCATCTCCATCTGG	CTTG	0.6602	115	100	+	128	8	50936198
+DPM1	CRISPRko	CTGTTAAATCAGATGCTCCTGGT	CTTC	0.6833	139	101	+	116	7	50940896
+DPM1	CRISPRko	ATATTGTCTCTGGAACTCGCTAC	TTTG	0.8469	318	102	-	28	6	50942083
+DPM1	CRISPRko	CTCCCAACTTGGATTCACCATAA	TTTC	0.8272	308	103	+	34	9	50935209
+DPM1	CRISPRko	AAACTAGGTTCCAATATCATTTG	TTTT	0.7819	281	104	-	49	9	50935223
+DPM1	CRISPRko	TTAACTCAGATCTTGCTGAGACC	TTTT	0.6778	147	105	-	118	7	50940897
+DPM1	CRISPRko	TTTGGCAGCCGTGGGGCCAATTT	TTTA	0.8195	313	106	-	36	7	50940921
+DPM1	CRISPRko	GAAACACATTTTTCTATTAATTT	TTTA	0.8425	328	107	+	29	8	50936236
+DPM1	CRISPRko	ATTTCTTACAGATTATACCGAAA	TTTA	0.8354	324	108	-	31	8	50936253
+DPM1	CRISPRko	TAATTTCTTACAGATTATACCGA	TTTT	0.7363	242	109	-	72	8	50936255
+DPM1	CRISPRko	AACTCAGATCTTGCTGAGACCAG	TTTT	0.6975	179	110	-	104	7	50940895
+DPM1	CRISPRko	ACGACAGAACAAATATTCGGTGC	GTCC	0.5935	26	111	-	181	1	50958438
+DPM1	CRISPRko	GCAGCCGTGGGGCCAATTTTTTA	TTTG	0.8044	307	112	-	41	7	50940917
+DPM1	CRISPRko	CATCATCTATGATTATAATTTCA	CTTC	0.6638	137	113	+	126	2	50955272
+DPM1	CRISPRko	CACTGTACCTGAAACTTCCTGTT	TTTT	0.6733	149	114	+	121	7	50940878
+DPM1	CRISPRko	TGTTAAATCAGATGCTCCTGGTC	TTCC	0.6486	113	115	+	139	7	50940897
+DPM1	CRISPRko	CATCTGGGCTTCCATCATCTATG	GTTC	0.6768	153	116	+	119	2	50955260
+DPM1	CRISPRko	ACAGCTAATTTATTCTACAGGAA	ATTG	0.6494	116	117	-	138	6	50942126
+DPM1	CRISPRko	TTATAACTTACAATTCTGTCTGA	GTTC	0.7489	266	118	+	65	2	50955196
+DPM1	CRISPRko	ATTTCCTCCCAACTTGGATTCAC	TTTC	0.7982	310	119	+	44	9	50935204
+DPM1	CRISPRko	TAGCGAGTTCCAGAGACAATATC	TTTG	0.8037	317	120	+	42	6	50942103
+DPM1	CRISPRko	TCGCAGGTCTCGGCGGGAGCTGG	GTCC	0.5679	10	121	-	196	1	50958474
+DPM1	CRISPRko	TGGATCGTGTTTATGGTGAATCC	TTTG	0.8061	323	122	-	40	9	50935200
+DPM1	CRISPRko	ATCTGGGCTTCCATCATCTATGA	TTCC	0.7283	248	123	+	78	2	50955261
+DPM1	CRISPRko	TCTGGAACTCGCTACAAAGGAAA	TGTC	0.6247	97	124	-	154	6	50942075
+DPM1	CRISPRko	TTTTATGTAGTAGCAAAAAGAGT	TTTC	0.8102	331	125	+	39	9	50935151
+DPM1	CRISPRko	CATACCTCGCCAATAGTATAATT	GTTG	0.6132	86	126	+	162	8	50936164
+DPM1	CRISPRko	AATTTCTTACAGATTATACCGAA	TTTT	0.7119	222	127	-	94	8	50936254
+DPM1	CRISPRko	TATTAATTTCTCTAGAACTTCTT	TTTC	0.7928	321	128	+	45	8	50936250
+DPM1	CRISPRko	CACCAGCAGCCACACGATGAGCG	TTTT	0.5973	56	129	+	178	1	50958401
+DPM1	CRISPRko	AATTTCAGCCAAAATTTATTCCT	TTCA	0.6916	190	130	-	112	5	50945750
+DPM1	CRISPRko	TAGAGAAATTAATAGAAAAATGT	GTTC	0.801	334	131	-	43	8	50936222
+DPM1	CRISPRko	ATTTCTCTAGAACTTCTTTTCGG	ATTA	0.6439	138	132	+	141	8	50936255
+DPM1	CRISPRko	TTCTACAGGAAGCAAAAGGAGGG	TTTA	0.7917	330	133	-	46	6	50942114
+DPM1	CRISPRko	TCTTTTCAAATCCCAGCCATATA	TTTT	0.7079	223	134	+	100	6	50942063
+DPM1	CRISPRko	AATATTAGAAATAGTACCTACCT	TTCA	0.7171	256	135	+	86	5	50945737
+DPM1	CRISPRko	TAGGTAGGTAAAAGCACCGAATA	GTTG	0.5561	25	136	+	202	1	50958444
+DPM1	CRISPRko	GGCAAGACAGTTGAATTATACTA	TTCG	0.6121	100	137	-	165	8	50936158
+DPM1	CRISPRko	TTGAAAGGATTATTGACTCTTTT	TTTC	0.7729	329	138	-	53	9	50935147
+DPM1	CRISPRko	CAAATCCCAGCCATATACACCTC	TTTT	0.6123	107	139	+	164	6	50942069
+DPM1	CRISPRko	GATGCTGATCTCTCACACCATGT	TATG	0.5799	61	140	-	188	4	50945847
+DPM1	CRISPRko	TTTTTAAACTAGGTTCCAATATC	TTTT	0.7293	286	141	-	77	9	50935228
+DPM1	CRISPRko	GTAGCAAAAAGAGTCAATAATCC	TGTA	0.6569	176	142	+	133	9	50935160
+DPM1	CRISPRko	TCCAACTGTTCAGCAACATCCCT	CTTC	0.6699	199	143	+	122	2	50955232
+DPM1	CRISPRko	TTTTTTAAACTAGGTTCCAATAT	TTTT	0.7185	279	144	-	83	9	50935229
+DPM1	CRISPRko	AAAGGCTACGTCTTCCAGATGGA	TTCT	0.6246	136	145	-	155	8	50936193
+DPM1	CRISPRko	ACTTACAATTCTGTCTGACCCAT	TATA	0.6101	110	146	+	168	2	50955201
+DPM1	CRISPRko	TTGACTCTTTTTGCTACTACATA	ATTA	0.6936	230	147	-	111	9	50935135
+DPM1	CRISPRko	TGGTCTCAGCAAGATCTGAGTTA	CTCC	0.6098	114	148	+	169	7	50940915
+DPM1	CRISPRko	ATTCAACTGTCTTGCCCGAACAA	TATA	0.6143	134	149	+	160	8	50936184
+DPM1	CRISPRko	CCATAAACACGATCCACAAATGA	TTCA	0.6909	231	150	+	113	9	50935226
+DPM1	CRISPRko	TATAACTTACAATTCTGTCTGAC	TTCT	0.5544	51	151	+	203	2	50955197
+DPM1	CRISPRko	TACCTAATAAATTCAGGAATAAA	TACC	0.5721	75	152	+	193	5	50945755
+DPM1	CRISPRko	TCTCTGGAACTCGCTACAAAGGA	ATTG	0.6387	173	153	-	145	6	50942077
+DPM1	CRISPRko	AGATGGAGATGATTGTTCGGGCA	TTCC	0.6635	210	154	-	127	8	50936177
+DPM1	CRISPRko	TTTAAACTAGGTTCCAATATCAT	TTTT	0.6943	250	155	-	108	9	50935226
+DPM1	CRISPRko	GCGAGTTCCAGAGACAATATCAA	TGTA	0.5798	88	156	+	189	6	50942105
+DPM1	CRISPRko	CTCGCAGGTCTCGGCGGGAGCTG	AGTC	0.5178	11	157	-	230	1	50958475
+DPM1	CRISPRko	ACAGGAAACTACATCATTATTAT	TGCC	0.6742	236	158	-	120	4	50945871
+DPM1	CRISPRko	TTCTTTTCAAATTTCAGCCAAAA	TTTA	0.7169	303	159	-	88	5	50945759
+DPM1	CRISPRko	TCTATTAATTTCTCTAGAACTTC	TTTT	0.6339	182	160	+	149	8	50936248
+DPM1	CRISPRko	TTTTAAACTAGGTTCCAATATCA	TTTT	0.6955	273	161	-	106	9	50935227
+DPM1	CRISPRko	ACACATGTACCTACCTGATTATT	TGTA	0.6016	133	162	+	176	6	50942038
+DPM1	CRISPRko	TGGTGTGAGAGATCAGCATCCAT	TACA	0.5283	44	163	+	221	4	50945869
+DPM1	CRISPRko	TTTTTTTAAACTAGGTTCCAATA	TTTT	0.7104	296	164	-	96	9	50935230
+DPM1	CRISPRko	CAGCCATATACACCTCCATTTCC	ATCC	0.6415	211	165	+	142	6	50942076
+DPM1	CRISPRko	AGAAAGATACTATTTCATTTCCT	TTCA	0.704	294	166	+	102	9	50935188
+DPM1	CRISPRko	TAGTAGCAAAAAGAGTCAATAAT	TATG	0.6039	152	167	+	174	9	50935158
+DPM1	CRISPRko	AGTCGTAGTCCTCGCAGGTCTCG	AGTC	0.499	16	168	-	242	1	50958485
+DPM1	CRISPRko	GCTGGGATTTGAAAAGAAAAATA	TATG	0.6324	201	169	-	150	6	50942038
+DPM1	CRISPRko	ATCTGGAAGACGTAGCCTTTAGA	CTCC	0.5954	144	170	+	180	8	50936215
+DPM1	CRISPRko	ACAGGAAGCAAAAGGAGGGTAAT	TTCT	0.6909	276	171	-	114	6	50942110
+DPM1	CRISPRko	TTCGGTGCTTTTACCTACCTACA	AATA	0.5074	30	172	-	238	1	50958423
+DPM1	CRISPRko	GTGGACTGCGCACTTCCAGCTCC	TGTC	0.4919	12	173	+	247	1	50958478
+DPM1	CRISPRko	TAGTCCCAACTTTTTCTCTCGTG	TACC	0.6044	162	174	+	173	3	50948651
+DPM1	CRISPRko	TTTTTAGGAACTGCATATATTCA	TTCA	0.6956	300	175	-	105	4	50945910
+DPM1	CRISPRko	AGAGATCAGCATCCATAATAATG	TGTG	0.607	169	176	+	171	4	50945876
+DPM1	CRISPRko	ATCATCTATGATTATAATTTCAT	TTCC	0.6525	239	177	+	136	2	50955273
+DPM1	CRISPRko	GATGCTCCTGGTCTCAGCAAGAT	ATCA	0.5529	105	178	+	204	7	50940907
+DPM1	CRISPRko	ATGTAGTAGCAAAAAGAGTCAAT	TTTT	0.6529	249	179	+	135	9	50935155
+DPM1	CRISPRko	CCAGCCATATACACCTCCATTTC	AATC	0.5261	74	180	+	223	6	50942075
+DPM1	CRISPRko	TGAAAGGATTATTGACTCTTTTT	TTCT	0.6124	200	181	-	163	9	50935146
+DPM1	CRISPRko	TTCGGGCAAGACAGTTGAATTAT	ATTG	0.6146	209	182	-	159	8	50936162
+DPM1	CRISPRko	TATTCCTACAGCTTCTAAGACCA	TTCT	0.5885	160	183	-	184	3	50948653
+DPM1	CRISPRko	CGCCGAGACCTGCGAGGACTACG	CTCC	0.5867	156	184	+	186	1	50958501
+DPM1	CRISPRko	GCTCCCGCCGAGACCTGCGAGGA	TCCA	0.5175	67	185	+	231	1	50958496
+DPM1	CRISPRko	GATCGTGTTTATGGTGAATCCAA	TGTG	0.524	87	186	-	225	9	50935198
+DPM1	CRISPRko	GATTATACCGAAAAGAAGTTCTA	TACA	0.5564	141	187	-	200	8	50936243
+DPM1	CRISPRko	GTCGTAGTCCTCGCAGGTCTCGG	GTCA	0.4954	54	188	-	244	1	50958484
+DPM1	CRISPRko	TTCCATGAATATATGCAGTTCCT	TTCA	0.666	299	189	+	123	4	50945924
+DPM1	CRISPRko	AAAATTACCCTCCTTTTGCTTCC	TATC	0.6199	233	190	+	157	6	50942126
+DPM1	CRISPRko	ATAGATCTTCTCCAACTGTTCAG	ACCC	0.4851	41	191	+	254	2	50955222
+DPM1	CRISPRko	GCCGAGACCTGCGAGGACTACGA	TCCC	0.4918	53	192	+	248	1	50958502
+DPM1	CRISPRko	AGAGAAATTAATAGAAAAATGTG	TTCT	0.6941	333	193	-	109	8	50936221
+DPM1	CRISPRko	AGCCATATACACCTCCATTTCCT	TCCC	0.5283	111	194	+	220	6	50942077
+DPM1	CRISPRko	CCTACCTAATAAATTCAGGAATA	AGTA	0.4855	45	195	+	253	5	50945753
+DPM1	CRISPRko	GCATCCATAATAATGATGTAGTT	ATCA	0.5364	127	196	+	212	4	50945884
+DPM1	CRISPRko	CGACTTACCTAGTCCCAACTTTT	TACA	0.456	14	197	+	269	3	50948642
+DPM1	CRISPRko	GTGAATCCAAGTTGGGAGGAAAT	TATG	0.5186	106	198	-	227	9	50935185
+DPM1	CRISPRko	TTTCCTCCCAACTTGGATTCACC	TTCA	0.6045	216	199	+	172	9	50935205
+DPM1	CRISPRko	GCTGCTGGTGAAAAGCTTCTCCG	TGTG	0.558	165	200	-	199	1	50958369
+DPM1	CRISPRko	ATAGATGATGGAAGCCCAGATGG	AATC	0.501	84	201	-	241	2	50955240
+DPM1	CRISPRko	ATTTGTGGATCGTGTTTATGGTG	TATC	0.6141	246	202	-	161	9	50935205
+DPM1	CRISPRko	GATGGAGATGATTGTTCGGGCAA	TCCA	0.573	184	203	-	192	8	50936176
+DPM1	CRISPRko	AAAGGATTATTGACTCTTTTTGC	CTTG	0.6194	257	204	-	158	9	50935144
+DPM1	CRISPRko	TCTCGGAGAAGCTTTTCACCAGC	TACC	0.4782	59	205	+	257	1	50958385
+DPM1	CRISPRko	CGACAGAACAAATATTCGGTGCT	TCCA	0.4416	17	206	-	278	1	50958437
+DPM1	CRISPRko	CTGAGACCAGGAGCATCTGATTT	CTTG	0.5908	212	207	-	182	7	50940882
+DPM1	CRISPRko	ACTTACATGGTGTGAGAGATCAG	TACC	0.6364	282	208	+	147	4	50945862
+DPM1	CRISPRko	GTACCTACCTAATAAATTCAGGA	AATA	0.4631	47	209	+	265	5	50945750
+DPM1	CRISPRko	TACCTCGCCAATAGTATAATTCA	TGCA	0.4452	31	210	+	276	8	50936166
+DPM1	CRISPRko	GTGGAAATTTTCACTGTACCTGA	AGTA	0.5696	194	211	+	195	7	50940867
+DPM1	CRISPRko	TTAGAAATAGTACCTACCTAATA	AATA	0.5439	171	212	+	208	5	50945741
+DPM1	CRISPRko	CCGAAAAGAAGTTCTAGAGAAAT	TATA	0.5854	214	213	-	187	8	50936236
+DPM1	CRISPRko	GAGACAATATCAAAATTACCCTC	TCCA	0.5256	140	214	+	224	6	50942115
+DPM1	CRISPRko	CAAGTTGGGAGGAAATGAAATAG	AATC	0.4833	80	215	-	255	9	50935178
+DPM1	CRISPRko	CGAACAATCATCTCCATCTGGAA	TGCC	0.5411	174	216	+	209	8	50936200
+DPM1	CRISPRko	GAAAAGAAGTTCTAGAGAAATTA	TACC	0.6295	290	217	-	152	8	50936234
+DPM1	CRISPRko	AAATTACCCTCCTTTTGCTTCCT	ATCA	0.5186	143	218	+	228	6	50942127
+DPM1	CRISPRko	GCTCATCGTGTGGCTGCTGGTGA	TGCC	0.4597	71	219	-	266	1	50958381
+DPM1	CRISPRko	ACTCTTTTTGCTACTACATAAAA	ATTG	0.5906	240	220	-	183	9	50935132
+DPM1	CRISPRko	GATCTTGCTGAGACCAGGAGCAT	CTCA	0.4899	104	221	-	251	7	50940889
+DPM1	CRISPRko	GTTCCGCCATGGCCTCCTTGGAA	CTCA	0.4515	64	222	-	272	1	50958511
+DPM1	CRISPRko	GTTGCATACCTCGCCAATAGTAT	ATCA	0.4718	85	223	+	263	8	50936160
+DPM1	CRISPRko	AAGTTGGGAGGAAATGAAATAGT	ATCC	0.6117	280	224	-	167	9	50935177
+DPM1	CRISPRko	CACCTCCATTTCCTTTGTAGCGA	TATA	0.5317	181	225	+	217	6	50942086
+DPM1	CRISPRko	TTGTTCCATCTGGGCTTCCATCA	TCCC	0.5203	163	226	+	226	2	50955254
+DPM1	CRISPRko	TCGTGTGGCTGCTGGTGAAAAGC	CTCA	0.4486	65	227	-	275	1	50958376
+DPM1	CRISPRko	TTTTCAAATTTCAGCCAAAATTT	ATTC	0.6028	271	228	-	175	5	50945756
+DPM1	CRISPRko	TGACCCATAGATCTTCTCCAACT	TGTC	0.4154	39	229	+	291	2	50955216
+DPM1	CRISPRko	TGCCGCTCATCGTGTGGCTGCTG	AACC	0.3968	15	230	-	303	1	50958385
+DPM1	CRISPRko	TACAACGAGCGCGAGAACCTGCC	TACC	0.3849	3	231	-	311	1	50958404
+DPM1	CRISPRko	GAAGTCAGTCGTAGTCCTCGCAG	CTTG	0.4035	29	232	-	298	1	50958491
+DPM1	CRISPRko	ATTCTGTCTGACCCATAGATCTT	TACA	0.4777	108	233	+	259	2	50955208
+DPM1	CRISPRko	GGTCTCGGCGGGAGCTGGAAGTG	CGCA	0.3899	13	234	-	308	1	50958469
+DPM1	CRISPRko	GAATATTTGTTCTGTCGTGGACT	CACC	0.4066	37	235	+	297	1	50958462
+DPM1	CRISPRko	CTGAACAGTTGGAGAAGATCTAT	GTTG	0.5717	244	236	-	194	2	50955203
+DPM1	CRISPRko	AATAGTATAATTCAACTGTCTTG	CGCC	0.5293	195	237	+	219	8	50936175
+DPM1	CRISPRko	AAGACCACGAGAGAAAAAGTTGG	TTCT	0.4126	49	238	-	293	3	50948637
+DPM1	CRISPRko	CCTACCTGATTATTTTTCTTTTC	TGTA	0.5785	255	239	+	191	6	50942047
+DPM1	CRISPRko	TACAGATTATACCGAAAAGAAGT	TTCT	0.5789	258	240	-	190	8	50936247
+DPM1	CRISPRko	TTGGAAGTCAGTCGTAGTCCTCG	CTCC	0.4258	70	241	-	284	1	50958494
+DPM1	CRISPRko	TTTCAAATCCCAGCCATATACAC	TTCT	0.5637	247	242	+	197	6	50942066
+DPM1	CRISPRko	TCTCCATCTGGAAGACGTAGCCT	ATCA	0.4506	98	243	+	273	8	50936210
+DPM1	CRISPRko	CCAACTGTTCAGCAACATCCCTT	TTCT	0.3922	32	244	+	306	2	50955233
+DPM1	CRISPRko	ACGAGCGCGAGAACCTGCCGCTC	TACA	0.3647	8	245	-	321	1	50958400
+DPM1	CRISPRko	ATAAACACGATCCACAAATGATA	CACC	0.5116	187	246	+	233	9	50935228
+DPM1	CRISPRko	AGCAGCCACACGATGAGCGGCAG	CACC	0.4026	55	247	+	300	1	50958405
+DPM1	CRISPRko	GCAAGATCTGAGTTAAAAAATTG	CTCA	0.5079	185	248	+	236	7	50940923
+DPM1	CRISPRko	TGAAACTTCCTGTTAAATCAGAT	TACC	0.535	232	249	+	213	7	50940887
+DPM1	CRISPRko	TTATGGATGCTGATCTCTCACAC	ATTA	0.4941	170	250	-	245	4	50945852
+DPM1	CRISPRko	TCTGGGCTTCCATCATCTATGAT	TCCA	0.5313	226	251	+	218	2	50955262
+DPM1	CRISPRko	TTTATGTAGTAGCAAAAAGAGTC	TTCT	0.5515	253	252	+	205	9	50935152
+DPM1	CRISPRko	CCTGAAACTTCCTGTTAAATCAG	TGTA	0.504	186	253	+	240	7	50940885
+DPM1	CRISPRko	TGGCTGGGATTTGAAAAGAAAAA	TATA	0.5636	270	254	-	198	6	50942040
+DPM1	CRISPRko	CAGATGGAACAAGGGATGTTGCT	AGCC	0.4558	128	255	-	270	2	50955224
+DPM1	CRISPRko	TATGGCTGGGATTTGAAAAGAAA	TGTA	0.5563	269	256	-	201	6	50942042
+DPM1	CRISPRko	GTAGTCCTCGCAGGTCTCGGCGG	AGTC	0.3378	9	257	-	331	1	50958481
+DPM1	CRISPRko	TTTCAAGAAAGATACTATTTCAT	ATCC	0.5406	252	258	+	210	9	50935183
+DPM1	CRISPRko	AATAATCCTTTCAAGAAAGATAC	AGTC	0.5328	245	259	+	215	9	50935175
+DPM1	CRISPRko	CAAATTTCAGCCAAAATTTATTC	TTTT	0.5326	243	260	-	216	5	50945752
+DPM1	CRISPRko	GTTTCCTGTGGCATGTTTCATTC	TGTA	0.4907	180	261	+	249	4	50945904
+DPM1	CRISPRko	GAGACCTGCGAGGACTACGACTG	CGCC	0.3814	52	262	+	314	1	50958505
+DPM1	CRISPRko	ATACAAATTTAATTTTTTTAGTG	ATTA	0.6096	342	263	-	170	2	50955286
+DPM1	CRISPRko	TCTGGAAGACGTAGCCTTTAGAA	TCCA	0.4219	112	264	+	287	8	50936216
+DPM1	CRISPRko	TTTCTAAAGGCTACGTCTTCCAG	TGTG	0.5104	217	265	-	235	8	50936198
+DPM1	CRISPRko	TTTCTTTTATGTAGTAGCAAAAA	TATC	0.5333	260	266	+	214	9	50935147
+DPM1	CRISPRko	GTATCTTTCTTGAAAGGATTATT	AATA	0.4223	117	267	-	286	9	50935156
+DPM1	CRISPRko	CACGACAGAACAAATATTCGGTG	AGTC	0.3556	38	268	-	326	1	50958439
+DPM1	CRISPRko	ACTGTTCAGCAACATCCCTTGTT	TCCA	0.363	46	269	+	323	2	50955236
+DPM1	CRISPRko	TTTTTAATTTCTTACAGATTATA	TATA	0.5963	335	270	-	179	8	50936259
+DPM1	CRISPRko	AGTTGGGAGGAAATGAAATAGTA	TCCA	0.5375	274	271	-	211	9	50935176
+DPM1	CRISPRko	ACTATGAAATTATAATCATAGAT	ATCA	0.5445	285	272	-	207	2	50955257
+DPM1	CRISPRko	GCTTCTAAGACCACGAGAGAAAA	TACA	0.3883	81	273	-	310	3	50948643
+DPM1	CRISPRko	TTCATGGAATGAAACATGCCACA	TATA	0.4903	203	274	-	250	4	50945891
+DPM1	CRISPRko	AACTGTTCAGCAACATCCCTTGT	CTCC	0.4981	218	275	+	243	2	50955235

resources/KO_EN_mouse.txt

@@ -0,0 +1,1001 @@
+Target Gene Symbol	CRISPR Mechanism	sgRNA Sequence	PAM Sequence	On-Target Efficacy Score	Off-Target Rank	Combined Rank	Strand of sgRNA	On-Target Rank	Exon Number	sgRNA Cut Position (1-based)
+Gnai3	CRISPRko	ATGATGACATCCGTAACAGCATC	TTTA	0.9669	47	1	+	2	8	108109477
+Gnai3	CRISPRko	CTATGCCATCCTCTCAATAAAGC	TTTA	0.9308	60	2	+	5	8	108109430
+Gnai3	CRISPRko	AATACATACGAAGAGGCAGCTGC	TTCC	0.9375	64	3	-	4	8	108109587
+Gnai3	CRISPRko	CCTTTTACTATGCCATCCTCTCA	TTTA	0.8845	57	4	+	18	8	108109423
+Gnai3	CRISPRko	CATTCGTCCTCTGAATAGCCGTC	TTTA	0.8621	67	5	+	24	3	108123669
+Gnai3	CRISPRko	TACCCCAATCGTCAACATACCAT	TTTC	0.8273	56	6	+	38	6	108112474
+Gnai3	CRISPRko	ATCACGCCTGCTAGTTCTGAAGT	TTTA	0.9032	109	7	+	12	4	108118403
+Gnai3	CRISPRko	CCTGGACCTGCTAAAGCATGCCT	ATTC	0.8084	36	8	+	49	4	108118351
+Gnai3	CRISPRko	ACGATGGTACTTTTACCAGATTC	TTTC	0.919	116	9	+	10	2	108123828
+Gnai3	CRISPRko	GCTACCTAAATGATTTGGATAGA	TTTA	0.9378	136	10	-	3	5	108115872
+Gnai3	CRISPRko	TGGCGAGATGGCGGGGTACAGGC	TTTA	0.8693	102	11	-	20	4	108118351
+Gnai3	CRISPRko	CCCTTACTCAGAATGTTTGATGT	TTTC	0.847	88	12	-	31	6	108112593
+Gnai3	CRISPRko	TAATGATGACATCCGTAACAGCA	TTTT	0.8079	53	13	+	50	8	108109475
+Gnai3	CRISPRko	CTGGACCTGCTAAAGCATGCCTG	TTCC	0.8642	110	14	+	22	4	108118352
+Gnai3	CRISPRko	AGAACTAGCAGGCGTGATTAAAC	CTTC	0.7489	17	15	-	71	4	108118379
+Gnai3	CRISPRko	AAGAATCATTCATGAGGACGGCT	TTTG	0.8125	69	16	-	46	3	108123666
+Gnai3	CRISPRko	GGCGCTGGAGAATCTGGTAAAAG	TTTA	0.9207	145	17	-	8	2	108123818
+Gnai3	CRISPRko	AACCGTCCCATGGCTCGTATGAT	CTTC	0.7593	31	18	+	66	3	108123594
+Gnai3	CRISPRko	TCCCCGTCCTCCCGCAAGTTGCG	TTTC	0.795	59	19	+	53	1	108145944
+Gnai3	CRISPRko	TTCGGTTCAGATCTTCAAACTGG	TTTC	0.8152	84	20	+	43	8	108109571
+Gnai3	CRISPRko	AGCACTCCCAGCTAAAACAAATA	CTTC	0.7413	20	21	+	76	4	108118441
+Gnai3	CRISPRko	CTCATCCTCAGCCAGAACAAGGT	TTTC	0.8687	137	22	+	21	6	108112500
+Gnai3	CRISPRko	AGTCCATCATTGCAATCATACGA	ATTC	0.7568	48	23	-	67	3	108123589
+Gnai3	CRISPRko	GCAGGTCCAGGGAATATCAGCTC	TTTA	0.8569	143	24	-	27	4	108118320
+Gnai3	CRISPRko	GATAGAATATCCCAGACCAACTA	TTTG	0.92	180	25	-	9	5	108115855
+Gnai3	CRISPRko	GAGGGAGTGACAGCAATTATCTT	TTTT	0.8274	134	26	-	37	6	108112527
+Gnai3	CRISPRko	AACGTTTATGGCGAGATGGCGGG	ATTA	0.7147	26	27	-	94	4	108118359
+Gnai3	CRISPRko	TCAGAACCGAATGCATGAAAGCA	TTTT	0.8297	152	28	-	34	7	108112072
+Gnai3	CRISPRko	TGGATAACAGATTGTTAATGGAC	ATTC	0.764	96	29	+	63	7	108111966
+Gnai3	CRISPRko	TGAGGACGGCTATTCAGAGGACG	TTCA	0.7424	78	30	-	75	3	108123654
+Gnai3	CRISPRko	TCGTTCGGATCTTTGGCCACCTA	TTTT	0.6882	11	31	+	113	6	108112590
+Gnai3	CRISPRko	CCAGACCAACTACATTCCAACTC	TATC	0.7266	66	32	-	87	5	108115844
+Gnai3	CRISPRko	ACTGTTTTGAGGGAGTGACAGCA	ATTC	0.7386	90	33	-	78	6	108112535
+Gnai3	CRISPRko	TTTAGCTACCTAAATGATTTGGA	TTTA	0.8848	214	34	-	17	5	108115876
+Gnai3	CRISPRko	GGTTCAGATCTTCAAACTGGCAC	CTTC	0.7008	46	35	+	103	8	108109574
+Gnai3	CRISPRko	GAACTAGCAGGCGTGATTAAACG	TTCA	0.6724	16	36	-	123	4	108118378
+Gnai3	CRISPRko	CAGACACTTCAATCATTCTCTTT	TTTA	0.8578	212	37	-	26	7	108112010
+Gnai3	CRISPRko	GAGGACGAATGTAAACAGTATAA	TTCA	0.7566	129	38	-	68	3	108123638
+Gnai3	CRISPRko	AGAGGACGAATGTAAACAGTATA	ATTC	0.8947	240	39	-	14	3	108123639
+Gnai3	CRISPRko	TAGGCGCTGGAGAATCTGGTAAA	TTTT	0.8323	202	40	-	33	2	108123820
+Gnai3	CRISPRko	TCATCCTCAGCCAGAACAAGGTC	TTCC	0.7917	164	41	+	54	6	108112501
+Gnai3	CRISPRko	CTTGAAGGTGAAGTGGGTCTCCA	GTTC	0.8126	183	42	+	45	5	108115798
+Gnai3	CRISPRko	TGTGGCTCTCAGTGATTACGACC	TTTG	0.8388	211	43	-	32	6	108112501
+Gnai3	CRISPRko	AGGGAATATCAGCTCAATGATTC	GTCC	0.6971	71	44	-	105	4	108118312
+Gnai3	CRISPRko	GCTGGGAGTGCTGAAGAAGGAGT	TTTA	0.9708	284	45	-	1	4	108118411
+Gnai3	CRISPRko	CCTGTGTATTCTGGATAACAGAT	TTTA	0.8618	239	46	+	25	7	108111955
+Gnai3	CRISPRko	TTAGGCGCTGGAGAATCTGGTAA	TTTT	0.7609	162	47	-	65	2	108123821
+Gnai3	CRISPRko	AGAACCGAATGCATGAAAGCATG	TTTC	0.9243	279	48	-	7	7	108112070
+Gnai3	CRISPRko	AATGATGACATCCGTAACAGCAT	TTTT	0.8171	219	49	+	42	8	108109476
+Gnai3	CRISPRko	CGACCTTGTTCTGGCTGAGGATG	ATTA	0.6666	54	50	-	126	6	108112483
+Gnai3	CRISPRko	TTCGGACAAGAGTGAAGACTACA	GTTC	0.7984	204	51	-	51	5	108115809
+Gnai3	CRISPRko	TTGTTACAAATGCTGTCAAACAA	TTTG	0.8115	216	52	+	47	7	108112063
+Gnai3	CRISPRko	CCCCTTACTCAGAATGTTTGATG	TTTT	0.6705	65	53	-	124	6	108112594
+Gnai3	CRISPRko	GCTCCACCGCCGCCTTGTCCTCG	CTCC	0.6515	39	54	+	138	1	108145984
+Gnai3	CRISPRko	TAACAACAAATGGTTTACAGACA	TTTG	0.8557	263	55	-	28	7	108112027
+Gnai3	CRISPRko	ATGGCTCGTATGATTGCAATGAT	TCCC	0.6876	89	56	+	115	3	108123603
+Gnai3	CRISPRko	GCCGCTTTCTCCCCGTCCTCCCG	TTTG	0.7377	161	57	+	81	1	108145935
+Gnai3	CRISPRko	CATTCAGTGCCAGTTTGAAGATC	CTTA	0.7546	190	58	-	69	8	108109561
+Gnai3	CRISPRko	CCTTACTCAGAATGTTTGATGTA	TTCC	0.8186	246	59	-	41	6	108112592
+Gnai3	CRISPRko	ACTCTTGTCCGAAGAACATCTTG	CTTC	0.738	171	60	+	80	5	108115838
+Gnai3	CRISPRko	AAAGGCCCTGTGTGAACTTACTT	CTTA	0.8537	288	61	+	29	2	108123795
+Gnai3	CRISPRko	CAATACATACGAAGAGGCAGCTG	GTTC	0.693	127	62	-	111	8	108109588
+Gnai3	CRISPRko	AAACTGGCACTGAATGTAAGCAG	CTTC	0.7744	233	63	+	59	8	108109587
+Gnai3	CRISPRko	TGATGCTGTTACGGATGTCATCA	TTTT	0.7262	178	64	-	88	8	108109459
+Gnai3	CRISPRko	TACTGTTTACATTCGTCCTCTGA	TTTA	0.7442	208	65	+	73	3	108123660
+Gnai3	CRISPRko	AGATCTTCAAACTGGCACTGAAT	GTTC	0.6881	128	66	+	114	8	108109579
+Gnai3	CRISPRko	ATGGGACGGTTGAAGATTGATTT	AGCC	0.6158	23	67	-	169	3	108123563
+Gnai3	CRISPRko	GTTGGTCTGGGATATTCTATCCA	TGTA	0.6406	63	68	+	150	5	108115876
+Gnai3	CRISPRko	AGGGAGTGACAGCAATTATCTTT	TTTG	0.9021	339	69	-	13	6	108112526
+Gnai3	CRISPRko	TCGGCGCTCAACGTGCAGCCCAT	GTCC	0.6551	101	70	+	134	1	108146004
+Gnai3	CRISPRko	CTCGGCGCTCAACGTGCAGCCCA	TGTC	0.651	99	71	+	139	1	108146003
+Gnai3	CRISPRko	CGGGAGGACGGGGAGAAAGCGGC	CTTG	0.6331	70	72	-	155	1	108145916
+Gnai3	CRISPRko	CTATGAAGCAGAATCATTGAGCT	CTTA	0.7071	187	73	+	98	4	108118321
+Gnai3	CRISPRko	CCCAAAATCAATCTTCAACCGTC	ATTC	0.6972	176	74	+	104	3	108123578
+Gnai3	CRISPRko	AAGATCTGAACCGAAGAAAAGAT	TTTG	0.851	326	75	-	30	8	108109544
+Gnai3	CRISPRko	ATGCTGTTACGGATGTCATCATT	TTTG	0.7823	282	76	-	55	8	108109457
+Gnai3	CRISPRko	GCACTCCCAGCTAAAACAAATAA	TTCA	0.6934	174	77	+	110	4	108118442
+Gnai3	CRISPRko	GAACCGAATGCATGAAAGCATGA	TTCA	0.7665	273	78	-	62	7	108112069
+Gnai3	CRISPRko	CCAAAATCAATCTTCAACCGTCC	TTCC	0.7203	223	79	+	91	3	108123579
+Gnai3	CRISPRko	GTCCATCATTGCAATCATACGAG	TTCA	0.6529	133	80	-	137	3	108123588
+Gnai3	CRISPRko	GAAGAATCATTCATGAGGACGGC	TTTT	0.7723	290	81	-	60	3	108123667
+Gnai3	CRISPRko	ATTGCAATGATGGACTGAATAGT	TATG	0.6318	97	82	+	157	3	108123615
+Gnai3	CRISPRko	CAGCTAAAACAAATAACTGTCGG	CTCC	0.6533	140	83	+	136	4	108118449
+Gnai3	CRISPRko	TCCGAAGAACATCTTGCTGAGTT	CTTG	0.6597	151	84	+	131	5	108115845
+Gnai3	CRISPRko	CGATGGTACTTTTACCAGATTCT	TTCA	0.6651	160	85	+	127	2	108123829
+Gnai3	CRISPRko	GAACGAAAAAAGTGGATTCACTG	ATCC	0.6425	125	86	-	148	6	108112554
+Gnai3	CRISPRko	GTTCGGATCTTTGGCCACCTACA	TTTC	0.9154	410	87	+	11	6	108112592
+Gnai3	CRISPRko	CCTGTGCCACAGACACCAAAAAT	TTCA	0.6942	217	88	-	108	8	108109496
+Gnai3	CRISPRko	GATCTTCAAACTGGCACTGAATG	TTCA	0.7297	261	89	+	86	8	108109580
+Gnai3	CRISPRko	TGTACCCCGCCATCTCGCCATAA	TGCC	0.6258	115	90	+	160	4	108118373
+Gnai3	CRISPRko	GGACAAGAGTGAAGACTACAGGC	CTTC	0.6969	224	91	-	106	5	108115806
+Gnai3	CRISPRko	GTCTGGGATATTCTATCCAAATC	GTTG	0.6038	68	92	+	185	5	108115880
+Gnai3	CRISPRko	ACCCCAATCGTCAACATACCATT	TTCT	0.5798	33	93	+	206	6	108112475
+Gnai3	CRISPRko	GGTGGCCAAAGATCCGAACGAAA	TGTA	0.6225	117	94	-	164	6	108112569
+Gnai3	CRISPRko	CCGCCATCTCGCCATAAACGTTT	TACC	0.5594	5	95	+	222	4	108118379
+Gnai3	CRISPRko	TTAATAAGAAAGACCTTTTTGAG	TTTC	0.8108	356	96	-	48	7	108111986
+Gnai3	CRISPRko	TCGGTTCAGATCTTCAAACTGGC	TTCT	0.614	111	97	+	171	8	108109572
+Gnai3	CRISPRko	TCCTTTTTGAAGAATCATTCATG	TTTC	0.8191	374	98	-	40	3	108123675
+Gnai3	CRISPRko	ACCGTCCCATGGCTCGTATGATT	TTCA	0.5582	2	99	+	227	3	108123595
+Gnai3	CRISPRko	CAGACCAACTACATTCCAACTCA	ATCC	0.5949	75	100	-	191	5	108115843
+Gnai3	CRISPRko	TTTTTCAGAACCGAATGCATGAA	TTTG	0.7237	281	101	-	89	7	108112076
+Gnai3	CRISPRko	TCAGGATGATGCCCGACAGTTAT	TTTC	0.9301	448	102	-	6	4	108118442
+Gnai3	CRISPRko	ACCTTTTACTATGCCATCCTCTC	TTTT	0.6807	221	103	+	120	8	108109422
+Gnai3	CRISPRko	TCAAAAAGGTCTTTCTTATTAAG	TTCC	0.8287	389	104	+	36	7	108112006
+Gnai3	CRISPRko	ATGTAGGTGGCCAAAGATCCGAA	TTTG	0.8633	416	105	-	23	6	108112574
+Gnai3	CRISPRko	CTGCTCTGGCAGATTCCCCAAAA	CTTA	0.7227	283	106	+	90	3	108123562
+Gnai3	CRISPRko	CGGATGTCATCATTAAAAACAAC	GTTA	0.7033	264	107	-	101	8	108109448
+Gnai3	CRISPRko	GGATAACAGATTGTTAATGGACT	TTCT	0.6417	169	108	+	149	7	108111967
+Gnai3	CRISPRko	AAGGAGGTCTACACTCACTTCAC	TACC	0.7099	278	109	-	95	8	108109518
+Gnai3	CRISPRko	AACTCAGCAAGATGTTCTTCGGA	TTCC	0.686	234	110	-	117	5	108115826
+Gnai3	CRISPRko	AATGCTGTCAAACAATTTCATGC	TACA	0.592	81	111	+	194	7	108112071
+Gnai3	CRISPRko	GCGAGATGGCGGGGTACAGGCAT	TATG	0.5541	9	112	-	231	4	108118349
+Gnai3	CRISPRko	ACAGCATTTGTAACAACAAATGG	TTTG	0.8874	440	113	-	16	7	108112037
+Gnai3	CRISPRko	AGGAAAAAATAAAGAGGAGTCCA	TTTG	0.8905	444	114	-	15	7	108111965
+Gnai3	CRISPRko	GTGTCTGTGGCACAGGTGAAGTG	TTTG	0.874	439	115	+	19	8	108109522
+Gnai3	CRISPRko	CCTCAAAAAGGTCTTTCTTATTA	TTTT	0.7317	307	116	+	85	7	108112004
+Gnai3	CRISPRko	TTATTAAGAAAGAGAATGATTGA	TTTC	0.8221	400	117	+	39	7	108112021
+Gnai3	CRISPRko	CAAATGCTGTCAAACAATTTCAT	GTTA	0.7667	360	118	+	61	7	108112069
+Gnai3	CRISPRko	ACTTCTTTGGCCGCTTTCTCCCC	CTTC	0.5831	80	119	+	201	1	108145926
+Gnai3	CRISPRko	AGTTGTTTTTAATGATGACATCC	TTTA	0.7756	368	120	+	58	8	108109466
+Gnai3	CRISPRko	GAGGAAAAAATAAAGAGGAGTCC	TTTT	0.8145	401	121	-	44	7	108111966
+Gnai3	CRISPRko	GAAGTCATGACTCCTTCTTCAGC	TTCT	0.6396	193	122	+	151	4	108118421
+Gnai3	CRISPRko	CTCAAAAAGGTCTTTCTTATTAA	TTTC	0.8293	428	123	+	35	7	108112005
+Gnai3	CRISPRko	TCTACAGCAATACTATTCAGTCC	GTTG	0.6053	135	124	-	182	3	108123607
+Gnai3	CRISPRko	ACTGAGAGCCACACAAAAGATAA	AATC	0.5805	92	125	+	204	6	108112530
+Gnai3	CRISPRko	GTGCCAGTTTGAAGATCTGAACC	TTCA	0.7062	301	126	-	100	8	108109555
+Gnai3	CRISPRko	TGGACCTGCTAAAGCATGCCTGT	TCCC	0.6356	201	127	+	152	4	108118353
+Gnai3	CRISPRko	TCCCCTTACTCAGAATGTTTGAT	TTTT	0.6936	289	128	-	109	6	108112595
+Gnai3	CRISPRko	TCACGCCCTTTACCTGTGTATTC	CACC	0.5626	74	129	+	218	7	108111943
+Gnai3	CRISPRko	TTTTAGCTGGGAGTGCTGAAGAA	TTTG	0.7333	346	130	-	82	4	108118416
+Gnai3	CRISPRko	TTTAGGCGCTGGAGAATCTGGTA	TTTT	0.6854	274	131	-	118	2	108123822
+Gnai3	CRISPRko	CAGAACCGAATGCATGAAAGCAT	TTTT	0.7454	367	132	-	72	7	108112071
+Gnai3	CRISPRko	GATGCTGTTACGGATGTCATCAT	TTTT	0.7981	408	133	-	52	8	108109458
+Gnai3	CRISPRko	GTCGTCTGCCTCAGGTTCCAATA	GTTG	0.6818	275	134	-	119	8	108109606
+Gnai3	CRISPRko	ACCTTCAAGGAACTCTACTTCAA	CTTC	0.6567	253	135	-	133	5	108115765
+Gnai3	CRISPRko	CGCCGCCGCCATGGGCTGCACGT	CTCC	0.5425	24	136	-	249	1	108145995
+Gnai3	CRISPRko	TGTGTATTCTGGATAACAGATTG	TACC	0.6536	255	137	+	135	7	108111957
+Gnai3	CRISPRko	ATTTAGCTACCTAAATGATTTGG	TTTT	0.743	378	138	-	74	5	108115877
+Gnai3	CRISPRko	GTCCTCTGAATAGCCGTCCTCAT	ATTC	0.5376	19	139	+	254	3	108123674
+Gnai3	CRISPRko	TTGAAGGTGAAGTGGGTCTCCAC	TTCC	0.7822	417	140	+	56	5	108115799
+Gnai3	CRISPRko	AGCTGGGAGTGCTGAAGAAGGAG	TTTT	0.7195	347	141	-	92	4	108118412
+Gnai3	CRISPRko	TTTTTGATGCTGTTACGGATGTC	TTTG	0.7012	329	142	-	102	8	108109463
+Gnai3	CRISPRko	AGACCAACTACATTCCAACTCAG	TCCC	0.6489	249	143	-	142	5	108115842
+Gnai3	CRISPRko	CAATGATGGACTGAATAGTATTG	ATTG	0.6449	243	144	+	145	3	108123619
+Gnai3	CRISPRko	GCTCTCAGTGATTACGACCTTGT	TGTG	0.5253	12	145	-	261	6	108112497
+Gnai3	CRISPRko	AGCAGCTGCCTCTTCGTATGTAT	TGTA	0.55	61	146	+	237	8	108109605
+Gnai3	CRISPRko	TGAAGAATCATTCATGAGGACGG	TTTT	0.5604	95	147	-	221	3	108123668
+Gnai3	CRISPRko	TTCGGATCTTTGGCCACCTACAT	TTCG	0.5889	150	148	+	195	6	108112593
+Gnai3	CRISPRko	GGTGTCTGTGGCACAGGTGAAGT	TTTT	0.7631	414	149	+	64	8	108109521
+Gnai3	CRISPRko	ACAGACACCAAAAATGTGCAGTT	TGCC	0.6099	194	150	-	177	8	108109488
+Gnai3	CRISPRko	ATAAGAAAGACCTTTTTGAGGAA	CTTA	0.7408	394	151	-	77	7	108111983
+Gnai3	CRISPRko	CTCTTGTCCGAAGAACATCTTGC	TTCA	0.5324	30	152	+	259	5	108115839
+Gnai3	CRISPRko	TGTGCCACAGACACCAAAAATGT	CACC	0.5886	157	153	-	196	8	108109494
+Gnai3	CRISPRko	ATGGACTCCTCTTTATTTTTTCC	GTTA	0.7068	351	154	+	99	7	108111983
+Gnai3	CRISPRko	AGAAAGAGAATGATTGAAGTGTC	ATTA	0.732	382	155	+	84	7	108112027
+Gnai3	CRISPRko	TTTTTCCCCTTACTCAGAATGTT	TTTT	0.6348	244	156	-	153	6	108112599
+Gnai3	CRISPRko	AAGGAATGTGGGCTTTATTGAGA	CTTA	0.7811	441	157	-	57	8	108109422
+Gnai3	CRISPRko	AATCGTCAACATACCATTTCCTC	CCCC	0.5178	18	158	+	271	6	108112480
+Gnai3	CRISPRko	TCGGACAAGAGTGAAGACTACAG	TTCT	0.6431	270	159	-	146	5	108115808
+Gnai3	CRISPRko	TGTAGTCTTCACTCTTGTCCGAA	TGCC	0.5528	98	160	+	233	5	108115828
+Gnai3	CRISPRko	AAGTGTCTGTAAACCATTTGTTG	ATTG	0.6039	197	161	+	184	7	108112043
+Gnai3	CRISPRko	AGGCGCTGGAGAATCTGGTAAAA	TTTT	0.7541	425	162	-	70	2	108123819
+Gnai3	CRISPRko	CTGTCACTCCCTCAAAACAGTGA	ATTG	0.598	191	163	+	188	6	108112556
+Gnai3	CRISPRko	ACCTGTGCCACAGACACCAAAAA	CTTC	0.7381	412	164	-	79	8	108109497
+Gnai3	CRISPRko	TGAAGTCATGACTCCTTCTTCAG	GTTC	0.6159	235	165	+	168	4	108118420
+Gnai3	CRISPRko	GAAGAACATCTTGCTGAGTTGGA	GTCC	0.5948	192	166	+	192	5	108115848
+Gnai3	CRISPRko	CCCCGTCCTCCCGCAAGTTGCGG	TTCT	0.5173	32	167	+	272	1	108145945
+Gnai3	CRISPRko	AGTGCCAGTTTGAAGATCTGAAC	ATTC	0.5779	163	168	-	207	8	108109556
+Gnai3	CRISPRko	ACTTTTTTCGTTCGGATCTTTGG	ATCC	0.5234	50	169	+	265	6	108112583
+Gnai3	CRISPRko	CGGTCGATCATCTTGCTCCGCTC	GTTG	0.5493	104	170	+	238	1	108145965
+Gnai3	CRISPRko	TTTCCCCTTACTCAGAATGTTTG	TTTT	0.6145	241	171	-	170	6	108112597
+Gnai3	CRISPRko	GTTGTCTACAGCAATACTATTCA	AGTA	0.5669	148	172	-	217	3	108123611
+Gnai3	CRISPRko	CTGTAGACAACTACTTTATACTG	ATTG	0.6307	268	173	+	158	3	108123642
+Gnai3	CRISPRko	TTAAGAAAGAGAATGATTGAAGT	CTTA	0.7086	391	174	+	97	7	108112024
+Gnai3	CRISPRko	ATTAACAATCTGTTATCCAGAAT	GTCC	0.5424	87	175	-	250	7	108111943
+Gnai3	CRISPRko	TTTTTTCCTCAAAAAGGTCTTTC	TTTA	0.6917	366	176	+	112	7	108111998
+Gnai3	CRISPRko	ACTGTTTTTCTCAGGATGATGCC	CTTA	0.6577	328	177	-	132	4	108118452
+Gnai3	CRISPRko	CCTTCAAGGAACTCTACTTCAAG	TTCA	0.6346	285	178	-	154	5	108115764
+Gnai3	CRISPRko	TCTGTTTCACGATGGTACTTTTA	TTCA	0.6071	237	179	+	178	2	108123820
+Gnai3	CRISPRko	TTTAAGTTGTTTTTAATGATGAC	TTCC	0.7094	403	180	+	96	8	108109462
+Gnai3	CRISPRko	ATAAACGTTTAATCACGCCTGCT	CGCC	0.4951	15	181	+	291	4	108118392
+Gnai3	CRISPRko	ATGAGGACGGCTATTCAGAGGAC	ATTC	0.5488	122	182	-	239	3	108123655
+Gnai3	CRISPRko	GTCCTCCCGCAAGTTGCGGTCGA	CCCC	0.4879	10	183	+	295	1	108145949
+Gnai3	CRISPRko	TTAATGGACTCCTCTTTATTTTT	ATTG	0.6034	229	184	+	186	7	108111980
+Gnai3	CRISPRko	TTTGACAGCATTTGTAACAACAA	ATTG	0.6253	280	185	-	161	7	108112041
+Gnai3	CRISPRko	TGGTAAAAGTACCATCGTGAAAC	AATC	0.5472	121	186	-	242	2	108123804
+Gnai3	CRISPRko	TTTTTAATGATGACATCCGTAAC	GTTG	0.6104	256	187	+	176	8	108109471
+Gnai3	CRISPRko	TGGTGTCTGTGGCACAGGTGAAG	TTTT	0.6601	353	188	+	129	8	108109520
+Gnai3	CRISPRko	TGAGGAAAAAATAAAGAGGAGTC	TTTT	0.6799	369	189	-	121	7	108111967
+Gnai3	CRISPRko	CTTACTATGAAGCAGAATCATTG	ATCA	0.5254	93	190	+	260	4	108118317
+Gnai3	CRISPRko	TTTGGCCGCTTTCTCCCCGTCCT	CTTC	0.6698	363	191	+	125	1	108145931
+Gnai3	CRISPRko	CCGTCCTCCCGCAAGTTGCGGTC	CTCC	0.5382	107	192	+	253	1	108145947
+Gnai3	CRISPRko	TTACAAATGCTGTCAAACAATTT	GTTG	0.5544	155	193	+	230	7	108112066
+Gnai3	CRISPRko	CTCAGGATGATGCCCGACAGTTA	TTTT	0.7331	449	194	-	83	4	108118443
+Gnai3	CRISPRko	TGGCTCTCAGTGATTACGACCTT	TGTG	0.4983	41	195	-	288	6	108112499
+Gnai3	CRISPRko	ACTCCCTCAAAACAGTGAATCCA	TGTC	0.507	62	196	+	278	6	108112561
+Gnai3	CRISPRko	TCATTGCAATCATACGAGCCATG	TCCA	0.4767	14	197	-	302	3	108123583
+Gnai3	CRISPRko	TTCGTCCTCTGAATAGCCGTCCT	TACA	0.5451	124	198	+	247	3	108123671
+Gnai3	CRISPRko	AGTTTGAAGATCTGAACCGAAGA	TGCC	0.6066	262	199	-	180	8	108109550
+Gnai3	CRISPRko	CTGAGTTGGAATGTAGTTGGTCT	CTTG	0.5618	185	200	+	220	5	108115861
+Gnai3	CRISPRko	TTTTTAGGCGCTGGAGAATCTGG	TTTT	0.6221	296	201	-	165	2	108123824
+Gnai3	CRISPRko	GACAAGAGTGAAGACTACAGGCA	TTCG	0.5042	72	202	-	280	5	108115805
+Gnai3	CRISPRko	TCTCAGGATGATGCCCGACAGTT	TTTT	0.7173	450	203	-	93	4	108118444
+Gnai3	CRISPRko	ACCGCCGCCTTGTCCTCGGCGCT	CTCC	0.5463	146	204	+	245	1	108145989
+Gnai3	CRISPRko	AATCATTCTCTTTCTTAATAAGA	CTTC	0.6641	381	205	-	128	7	108112000
+Gnai3	CRISPRko	CGTTCGGATCTTTGGCCACCTAC	TTTT	0.6863	406	206	+	116	6	108112591
+Gnai3	CRISPRko	CGAAGAACATCTTGCTGAGTTGG	TGTC	0.5725	213	207	+	213	5	108115847
+Gnai3	CRISPRko	TCCTCTGAATAGCCGTCCTCATG	TTCG	0.5588	188	208	+	226	3	108123675
+Gnai3	CRISPRko	AAGAGGAGTCCATTAACAATCTG	AATA	0.5044	83	209	-	279	7	108111954
+Gnai3	CRISPRko	CCCCGCCATCTCGCCATAAACGT	TGTA	0.4798	44	210	+	299	4	108118377
+Gnai3	CRISPRko	ATCATTGCAATCATACGAGCCAT	GTCC	0.4576	22	211	-	315	3	108123584
+Gnai3	CRISPRko	CTTCATCTGTTTCACGATGGTAC	CTTA	0.6949	438	212	+	107	2	108123815
+Gnai3	CRISPRko	AAGTAGTTGTCTACAGCAATACT	TATA	0.6134	305	213	-	174	3	108123616
+Gnai3	CRISPRko	CAAAATCAATCTTCAACCGTCCC	TCCC	0.5507	182	214	+	236	3	108123580
+Gnai3	CRISPRko	CTCCTTTTTGAAGAATCATTCAT	TTTT	0.6429	364	215	-	147	3	108123676
+Gnai3	CRISPRko	CCTCTCCCGCCGCCGCCATGGGC	GTCC	0.5702	228	216	-	215	1	108146002
+Gnai3	CRISPRko	GTTCAGATCTTCAAACTGGCACT	TTCG	0.6055	302	217	+	181	8	108109575
+Gnai3	CRISPRko	ATCCTCTCAATAAAGCCCACATT	TGCC	0.49	77	218	+	294	8	108109437
+Gnai3	CRISPRko	TTTTAGGCGCTGGAGAATCTGGT	TTTT	0.6321	354	219	-	156	2	108123823
+Gnai3	CRISPRko	CCAATCGTCAACATACCATTTCC	TACC	0.4511	28	220	+	320	6	108112478
+Gnai3	CRISPRko	TTCCTCAAAAAGGTCTTTCTTAT	TTTT	0.6135	323	221	+	173	7	108112002
+Gnai3	CRISPRko	TTCCCCTTACTCAGAATGTTTGA	TTTT	0.6496	387	222	-	141	6	108112596
+Gnai3	CRISPRko	TACGAGCCATGGGACGGTTGAAG	ATCA	0.443	7	223	-	333	3	108123571
+Gnai3	CRISPRko	TGTAAACCATTTGTTGTTACAAA	TGTC	0.673	430	224	+	122	7	108112050
+Gnai3	CRISPRko	CTCAGAATGTTTGATGTAGGTGG	CTTA	0.5563	218	225	-	228	6	108112587
+Gnai3	CRISPRko	CTTACTCAGAATGTTTGATGTAG	TCCC	0.6115	324	226	-	175	6	108112591
+Gnai3	CRISPRko	ACTATGCCATCCTCTCAATAAAG	TTTT	0.6136	334	227	+	172	8	108109429
+Gnai3	CRISPRko	CATGGCTCGTATGATTGCAATGA	GTCC	0.5032	112	228	+	283	3	108123602
+Gnai3	CRISPRko	TCCTCAAAAAGGTCTTTCTTATT	TTTT	0.6598	421	229	+	130	7	108112003
+Gnai3	CRISPRko	GTTTTTTTTTTAGGCGCTGGAGA	TTTT	0.5966	306	230	-	189	2	108123830
+Gnai3	CRISPRko	GACCTCCTTGGTATCTTTTCTTC	TGTA	0.5109	131	231	+	277	8	108109551
+Gnai3	CRISPRko	TTTTTTTTTTAGGCGCTGGAGAA	TTTG	0.6509	405	232	-	140	2	108123829
+Gnai3	CRISPRko	CTCCGCTCCACCGCCGCCTTGTC	CTTG	0.547	200	233	+	244	1	108145980
+Gnai3	CRISPRko	AACAGTATAAAGTAGTTGTCTAC	TGTA	0.5854	295	234	-	198	3	108123625
+Gnai3	CRISPRko	CTGTTTTGAGGGAGTGACAGCAA	TTCA	0.596	313	235	-	190	6	108112534
+Gnai3	CRISPRko	CATTAACAATCTGTTATCCAGAA	AGTC	0.4727	86	236	-	304	7	108111944
+Gnai3	CRISPRko	CATTTTATTTAGCTACCTAAATG	TATG	0.544	198	237	-	248	5	108115883
+Gnai3	CRISPRko	TACGAAGAGGCAGCTGCTTACAT	TACA	0.5592	252	238	-	223	8	108109581
+Gnai3	CRISPRko	GATCTTTGGCCACCTACATCAAA	TTCG	0.6453	411	239	+	144	6	108112597
+Gnai3	CRISPRko	TTTTCCCCTTACTCAGAATGTTT	TTTT	0.6068	341	240	-	179	6	108112598
+Gnai3	CRISPRko	TCTTTTGTGTGGCTCTCAGTGAT	ATTA	0.6023	330	241	-	187	6	108112508
+Gnai3	CRISPRko	GCCCCCAGCCCCTCACCGAGCAG	CTCC	0.5163	156	242	+	274	1	108145893
+Gnai3	CRISPRko	ATTCATGAGGACGGCTATTCAGA	AATC	0.444	45	243	-	331	3	108123659
+Gnai3	CRISPRko	TTTTTTCCCCTTACTCAGAATGT	TTTT	0.6182	377	244	-	167	6	108112600
+Gnai3	CRISPRko	ACTTCACCTTCAAGGAACTCTAC	ACCC	0.5398	210	245	-	252	5	108115770
+Gnai3	CRISPRko	GAATGTAGTTGGTCTGGGATATT	GTTG	0.5938	336	246	+	193	5	108115869
+Gnai3	CRISPRko	GGATGATGCCCGACAGTTATTTG	CTCA	0.5235	199	247	-	262	4	108118439
+Gnai3	CRISPRko	TTCGTTCGGATCTTTGGCCACCT	TTTT	0.4315	38	248	+	345	6	108112589
+Gnai3	CRISPRko	GTGTGGCTCTCAGTGATTACGAC	TTTT	0.5807	325	249	-	202	6	108112502
+Gnai3	CRISPRko	CGACAGTTATTTGTTTTAGCTGG	TGCC	0.4872	138	250	-	296	4	108118429
+Gnai3	CRISPRko	AAGTAGAGTTCCTTGAAGGTGAA	CTTG	0.5778	315	251	+	208	5	108115787
+Gnai3	CRISPRko	TCAGCCAGAACAAGGTCGTAATC	ATCC	0.4701	120	252	+	306	6	108112507
+Gnai3	CRISPRko	GCTCAATGATTCTGCTTCATAGT	ATCA	0.5235	206	253	-	263	4	108118301
+Gnai3	CRISPRko	CTCTGAATAGCCGTCCTCATGAA	CGTC	0.4315	40	254	+	346	3	108123677
+Gnai3	CRISPRko	CAGGATGATGCCCGACAGTTATT	TTCT	0.6467	447	255	-	143	4	108118441
+Gnai3	CRISPRko	ATTGAGCTGATATTCCCTGGACC	AATC	0.5471	250	256	+	243	4	108118336
+Gnai3	CRISPRko	ACACAAAAGATAATTGCTGTCAC	AGCC	0.5374	227	257	+	255	6	108112540
+Gnai3	CRISPRko	ATCTGTTTCACGATGGTACTTTT	CTTC	0.624	420	258	+	162	2	108123819
+Gnai3	CRISPRko	ATACGAGCCATGGGACGGTTGAA	AATC	0.3991	1	259	-	373	3	108123572
+Gnai3	CRISPRko	TCTCCCGCCGCCGCCATGGGCTG	CCCC	0.4955	168	260	-	290	1	108146000
+Gnai3	CRISPRko	TGTGGCACAGGTGAAGTGAGTGT	TGTC	0.6264	431	261	+	159	8	108109527
+Gnai3	CRISPRko	GAAGAAAAGATACCAAGGAGGTC	AACC	0.5846	352	262	-	199	8	108109532
+Gnai3	CRISPRko	TACAGCAATACTATTCAGTCCAT	TGTC	0.5681	320	263	-	216	3	108123605
+Gnai3	CRISPRko	ACAATGCCTGTAGTCTTCACTCT	CTCC	0.6193	422	264	+	166	5	108115820
+Gnai3	CRISPRko	TTAACAATCTGTTATCCAGAATA	TCCA	0.4514	118	265	-	319	7	108111942
+Gnai3	CRISPRko	ATACATACGAAGAGGCAGCTGCT	TCCA	0.4937	173	266	-	292	8	108109586
+Gnai3	CRISPRko	CTGTTTACATTCGTCCTCTGAAT	TATA	0.5757	335	267	+	211	3	108123662
+Gnai3	CRISPRko	TTTGTTTTAGCTGGGAGTGCTGA	GTTA	0.5589	311	268	-	224	4	108118420
+Gnai3	CRISPRko	TCAGCACTCCCAGCTAAAACAAA	TTCT	0.5035	196	269	+	282	4	108118439
+Gnai3	CRISPRko	ACTCAGCAAGATGTTCTTCGGAC	TCCA	0.5192	225	270	-	268	5	108115825
+Gnai3	CRISPRko	AAGATTGATTTTGGGGAATCTGC	GTTG	0.5335	245	271	-	258	3	108123551
+Gnai3	CRISPRko	ACAATCTGTTATCCAGAATACAC	ATTA	0.5777	344	272	-	209	7	108111939
+Gnai3	CRISPRko	ATTTTGGGGAATCTGCCAGAGCA	ATTG	0.5758	345	273	-	210	3	108123544
+Gnai3	CRISPRko	TTTTACTATGCCATCCTCTCAAT	TACC	0.5523	298	274	+	234	8	108109425
+Gnai3	CRISPRko	GAGACCCACTTCACCTTCAAGGA	TGTG	0.4669	149	275	-	309	5	108115777
+Gnai3	CRISPRko	TAATAAGAAAGACCTTTTTGAGG	TTCT	0.5806	362	276	-	203	7	108111985
+Gnai3	CRISPRko	CTCAAAACAGTGAATCCACTTTT	CTCC	0.5187	231	277	+	269	6	108112566
+Gnai3	CRISPRko	ATGATGGACTGAATAGTATTGCT	TGCA	0.4925	186	278	+	293	3	108123621
+Gnai3	CRISPRko	GACAACTACTTTATACTGTTTAC	TGTA	0.4076	42	279	+	366	3	108123647
+Gnai3	CRISPRko	CGTCCTCCCGCAAGTTGCGGTCG	TCCC	0.441	100	280	+	337	1	108145948
+Gnai3	CRISPRko	AACTGGCACTGAATGTAAGCAGC	TTCA	0.6049	409	281	+	183	8	108109588
+Gnai3	CRISPRko	TTTTTTAGGCGCTGGAGAATCTG	TTTT	0.5535	312	282	-	232	2	108123825
+Gnai3	CRISPRko	GTTTTGTTTTTTTTTTAGGCGCT	TTTT	0.6233	452	283	-	163	2	108123835
+Gnai3	CRISPRko	TATTAAGAAAGAGAATGATTGAA	TTCT	0.5858	388	284	+	197	7	108112022
+Gnai3	CRISPRko	TCCCAGACCAACTACATTCCAAC	AATA	0.4222	73	285	-	355	5	108115846
+Gnai3	CRISPRko	GCAAGATGTTCTTCGGACAAGAG	CTCA	0.5163	238	286	-	273	5	108115820
+Gnai3	CRISPRko	CGCCATCTCGCCATAAACGTTTA	ACCC	0.3821	8	287	+	390	4	108118380
+Gnai3	CRISPRko	GGTTCCAATACATACGAAGAGGC	CTCA	0.3956	37	288	-	378	8	108109593
+Gnai3	CRISPRko	TCCCGCAAGTTGCGGTCGATCAT	GTCC	0.3635	4	289	+	396	1	108145953
+Gnai3	CRISPRko	GCCGCCGCCATGGGCTGCACGTT	TCCC	0.4472	144	290	-	326	1	108145994
+Gnai3	CRISPRko	GAGCAGCAGCAGCTTCACTTCTT	CACC	0.4454	147	291	+	329	1	108145910
+Gnai3	CRISPRko	GTCTTCACTCTTGTCCGAAGAAC	TGTA	0.3958	52	292	+	377	5	108115832
+Gnai3	CRISPRko	ACTTACTATGAAGCAGAATCATT	AATC	0.4985	232	293	+	287	4	108118316
+Gnai3	CRISPRko	CCATGGCTCGTATGATTGCAATG	CGTC	0.3905	35	294	+	386	3	108123601
+Gnai3	CRISPRko	CTTTCTTAATAAGAAAGACCTTT	TTCT	0.5623	371	295	-	219	7	108111991
+Gnai3	CRISPRko	TCTTTCTTAATAAGAAAGACCTT	ATTC	0.5802	399	296	-	205	7	108111992
+Gnai3	CRISPRko	GGATCTTTGGCCACCTACATCAA	GTTC	0.4713	203	297	+	305	6	108112596
+Gnai3	CRISPRko	AATCTTCAACCGTCCCATGGCTC	AATC	0.4398	132	298	+	341	3	108123587
+Gnai3	CRISPRko	TCAGGTTCCAATACATACGAAGA	TGCC	0.52	287	299	-	266	8	108109596
+Gnai3	CRISPRko	ATCATTAAAAACAACTTAAAGGA	TGTC	0.5003	248	300	-	286	8	108109440
+Gnai3	CRISPRko	GCAAGTTGCGGTCGATCATCTTG	TCCC	0.413	103	301	+	359	1	108145957
+Gnai3	CRISPRko	CTTTAAGTTGTTTTTAATGATGA	ATTC	0.5753	398	302	+	212	8	108109461
+Gnai3	CRISPRko	AACTTACTTCATCTGTTTCACGA	TGTG	0.4076	94	303	+	365	2	108123809
+Gnai3	CRISPRko	TCAGCTCAATGATTCTGCTTCAT	AATA	0.4408	153	304	-	338	4	108118304
+Gnai3	CRISPRko	ATCATTCTCTTTCTTAATAAGAA	TTCA	0.5588	383	305	-	225	7	108111999
+Gnai3	CRISPRko	ACAGCACCTCACGCCCTTTACCT	TCCA	0.3958	82	306	+	376	7	108111935
+Gnai3	CRISPRko	AGCTCAATGATTCTGCTTCATAG	TATC	0.5842	434	307	-	200	4	108118302
+Gnai3	CRISPRko	GAATGTTTGATGTAGGTGGCCAA	CTCA	0.4432	172	308	-	332	6	108112583
+Gnai3	CRISPRko	CTTCGGTTCAGATCTTCAAACTG	TTTT	0.3654	49	309	+	394	8	108109570
+Gnai3	CRISPRko	TGCCTCAGGTTCCAATACATACG	CGTC	0.448	189	310	-	324	8	108109600
+Gnai3	CRISPRko	ACTTGCGGGAGGACGGGGAGAAA	CGCA	0.336	13	311	-	412	1	108145921
+Gnai3	CRISPRko	ATAAAGCCCACATTCCTTTAAGT	CTCA	0.5234	310	312	+	264	8	108109446
+Gnai3	CRISPRko	GCCAGAACAAGGTCGTAATCACT	CTCA	0.3445	27	313	+	407	6	108112510
+Gnai3	CRISPRko	TCTGAATAGCCGTCCTCATGAAT	GTCC	0.4745	236	314	+	303	3	108123678
+Gnai3	CRISPRko	TTCAGCACTCCCAGCTAAAACAA	CTTC	0.4617	222	315	+	312	4	108118438
+Gnai3	CRISPRko	TTCTGGATAACAGATTGTTAATG	TGTA	0.5473	365	316	+	241	7	108111963
+Gnai3	CRISPRko	ATCATACGAGCCATGGGACGGTT	TGCA	0.4403	170	317	-	339	3	108123575
+Gnai3	CRISPRko	CCTTTTTGAAGAATCATTCATGA	TTCT	0.5483	370	318	-	240	3	108123674
+Gnai3	CRISPRko	TTTTTTTCCCCTTACTCAGAATG	TTTT	0.5455	361	319	-	246	6	108112601
+Gnai3	CRISPRko	GCAATACTATTCAGTCCATCATT	TACA	0.4111	130	320	-	362	3	108123601
+Gnai3	CRISPRko	AATATGCATTTTATTTAGCTACC	AACC	0.5507	385	321	-	235	5	108115889
+Gnai3	CRISPRko	ATGACTTCAGAACTAGCAGGCGT	AGTC	0.4476	207	322	-	325	4	108118387
+Gnai3	CRISPRko	ATGGGCTGCACGTTGAGCGCCGA	CGCC	0.3976	108	323	-	375	1	108145985
+Gnai3	CRISPRko	CAACTCAGCAAGATGTTCTTCGG	ATTC	0.4356	177	324	-	343	5	108115827
+Gnai3	CRISPRko	TAAATGATTTGGATAGAATATCC	TACC	0.5038	303	325	-	281	5	108115866
+Gnai3	CRISPRko	GCCATCTCGCCATAAACGTTTAA	CCCC	0.4286	167	326	+	349	4	108118381
+Gnai3	CRISPRko	CCATCCTCTCAATAAAGCCCACA	TATG	0.3638	85	327	+	395	8	108109435
+Gnai3	CRISPRko	ATGACTCCTTCTTCAGCACTCCC	AGTC	0.4286	179	328	+	348	4	108118427
+Gnai3	CRISPRko	CTCCCGCAAGTTGCGGTCGATCA	CGTC	0.2958	21	329	+	427	1	108145952
+Gnai3	CRISPRko	GTAACAGCATCAAAAACAAACTG	ATCC	0.4969	299	330	+	289	8	108109489
+Gnai3	CRISPRko	TTCTTCAGCACTCCCAGCTAAAA	CTCC	0.4577	251	331	+	314	4	108118435
+Gnai3	CRISPRko	TTTTGTTTTTTTTTTAGGCGCTG	TTTG	0.5706	451	332	-	214	2	108123834
+Gnai3	CRISPRko	ACGTACTTGAAGTAGAGTTCCTT	TATG	0.3352	55	333	+	413	5	108115778
+Gnai3	CRISPRko	CGCCTGCTAGTTCTGAAGTCATG	ATCA	0.4115	159	334	+	361	4	108118407
+Gnai3	CRISPRko	CGCAAGTTGCGGTCGATCATCTT	CTCC	0.2632	3	335	+	441	1	108145956
+Gnai3	CRISPRko	TCAAAACAGTGAATCCACTTTTT	TCCC	0.4567	254	336	+	316	6	108112567
+Gnai3	CRISPRko	AGCTGATATTCCCTGGACCTGCT	ATTG	0.4229	181	337	+	353	4	108118340
+Gnai3	CRISPRko	CAATCATACGAGCCATGGGACGG	ATTG	0.42	175	338	-	357	3	108123577
+Gnai3	CRISPRko	ACAACAAATGGTTTACAGACACT	TGTA	0.5563	433	339	-	229	7	108112025
+Gnai3	CRISPRko	TGACTCCTTCTTCAGCACTCCCA	GTCA	0.3949	141	340	+	380	4	108118428
+Gnai3	CRISPRko	TTACTCAGAATGTTTGATGTAGG	CCCC	0.4548	271	341	-	317	6	108112590
+Gnai3	CRISPRko	CATCATTGCAATCATACGAGCCA	AGTC	0.221	6	342	-	450	3	108123585
+Gnai3	CRISPRko	TTTTGTGTGGCTCTCAGTGATTA	TATC	0.5148	357	343	-	275	6	108112506
+Gnai3	CRISPRko	AGTTGCGGTCGATCATCTTGCTC	CGCA	0.2699	29	344	+	439	1	108145960
+Gnai3	CRISPRko	GTCCCATGGCTCGTATGATTGCA	AACC	0.2627	25	345	+	442	3	108123598
+Gnai3	CRISPRko	AAAACAACTTAAAGGAATGTGGG	ATTA	0.5194	376	346	-	267	8	108109433
+Gnai3	CRISPRko	CATACGAAGAGGCAGCTGCTTAC	AATA	0.3235	76	347	-	417	8	108109583
+Gnai3	CRISPRko	GACACTTCAATCATTCTCTTTCT	TACA	0.4093	184	348	-	363	7	108112008
+Gnai3	CRISPRko	AACATACCATTTCCTCATCCTCA	CGTC	0.4484	269	349	+	321	6	108112487
+Gnai3	CRISPRko	ATTTGTTGTTACAAATGCTGTCA	AACC	0.4801	317	350	+	298	7	108112058
+Gnai3	CRISPRko	ACGCCTGCTAGTTCTGAAGTCAT	AATC	0.363	119	351	+	397	4	108118406
+Gnai3	CRISPRko	CCGCCGCCTTGTCCTCGGCGCTC	TCCA	0.291	58	352	+	428	1	108145990
+Gnai3	CRISPRko	CGCCCTTTACCTGTGTATTCTGG	CTCA	0.2702	43	353	+	438	7	108111946
+Gnai3	CRISPRko	AGCTAAAACAAATAACTGTCGGG	TCCC	0.4579	293	354	+	313	4	108118450
+Gnai3	CRISPRko	GTATTGCTGTAGACAACTACTTT	AATA	0.5023	358	355	+	284	3	108123636
+Gnai3	CRISPRko	CACTTTTTTCGTTCGGATCTTTG	AATC	0.2404	34	356	+	447	6	108112582
+Gnai3	CRISPRko	TTCACTCTTGTCCGAAGAACATC	AGTC	0.3371	113	357	+	410	5	108115835
+Gnai3	CRISPRko	ATTTCCTCATCCTCAGCCAGAAC	TACC	0.5414	432	358	+	251	6	108112495
+Gnai3	CRISPRko	GAGGACAAGGCGGCGGTGGAGCG	CGCC	0.5369	424	359	-	256	1	108145964
+Gnai3	CRISPRko	CGTTGAGCGCCGAGGACAAGGCG	TGCA	0.3267	106	360	-	415	1	108145975
+Gnai3	CRISPRko	CTTTACCTGTGTATTCTGGATAA	CGCC	0.4859	343	361	+	297	7	108111950
+Gnai3	CRISPRko	TCACCGAGCAGCAGCAGCTTCAC	CCCC	0.4786	340	362	+	300	1	108145905
+Gnai3	CRISPRko	TTGCAATCATACGAGCCATGGGA	ATCA	0.2012	51	363	-	452	3	108123580
+Gnai3	CRISPRko	CTGAGAGCCACACAAAAGATAAT	ATCA	0.5358	442	364	+	257	6	108112531
+Gnai3	CRISPRko	TTGGTATCTTTTCTTCGGTTCAG	CTCC	0.3944	195	365	+	381	8	108109558
+Gnai3	CRISPRko	CTTTTTTCGTTCGGATCTTTGGC	TCCA	0.3566	154	366	+	404	6	108112584
+Gnai3	CRISPRko	GTCAACATACCATTTCCTCATCC	AATC	0.2703	91	367	+	437	6	108112484
+Gnai3	CRISPRko	TGACTTCAGAACTAGCAGGCGTG	GTCA	0.4283	265	368	-	350	4	108118386
+Gnai3	CRISPRko	CAATCGTCAACATACCATTTCCT	ACCC	0.4223	259	369	+	354	6	108112479
+Gnai3	CRISPRko	CGGTTTTCTCCTTTTTGAAGAAT	CTTG	0.4655	350	370	-	310	3	108123683
+Gnai3	CRISPRko	GCCGCCTTGTCCTCGGCGCTCAA	CACC	0.2812	114	371	+	430	1	108145992
+Gnai3	CRISPRko	AAAATCAATCTTCAACCGTCCCA	CCCC	0.446	319	372	+	328	3	108123581
+Gnai3	CRISPRko	GTATCTTTTCTTCGGTTCAGATC	CTTG	0.2265	79	373	+	449	8	108109561
+Gnai3	CRISPRko	CCTCACCGAGCAGCAGCAGCTTC	AGCC	0.3463	165	374	+	406	1	108145903
+Gnai3	CRISPRko	TCTCAATAAAGCCCACATTCCTT	ATCC	0.4125	258	375	+	360	8	108109441
+Gnai3	CRISPRko	GGGAATATCAGCTCAATGATTCT	TCCA	0.4381	297	376	-	342	4	108118311
+Gnai3	CRISPRko	TCTTTATTTTTTCCTCAAAAAGG	CTCC	0.5181	445	377	+	270	7	108111992
+Gnai3	CRISPRko	CTATTCAGTCCATCATTGCAATC	AATA	0.2795	123	378	-	432	3	108123595
+Gnai3	CRISPRko	CTTCTTTGGCCGCTTTCTCCCCG	TTCA	0.5128	436	379	+	276	1	108145927
+Gnai3	CRISPRko	AAGCAGAATCATTGAGCTGATAT	TATG	0.3907	220	380	+	385	4	108118326
+Gnai3	CRISPRko	TTGTCCTCGGCGCTCAACGTGCA	CGCC	0.3052	142	381	+	424	1	108145998
+Gnai3	CRISPRko	TGCTAGTTCTGAAGTCATGACTC	CGCC	0.3757	209	382	+	391	4	108118411
+Gnai3	CRISPRko	CCGAGCAGCAGCAGCTTCACTTC	CTCA	0.2764	126	383	+	433	1	108145908
+Gnai3	CRISPRko	TTTTCTTCGGTTCAGATCTTCAA	TATC	0.4416	321	384	+	336	8	108109566
+Gnai3	CRISPRko	GTGATTACGACCTTGTTCTGGCT	CTCA	0.4313	300	385	-	347	6	108112490
+Gnai3	CRISPRko	CTCTCCCGCCGCCGCCATGGGCT	TCCC	0.4426	327	386	-	334	1	108146001
+Gnai3	CRISPRko	GCCGCCATGGGCTGCACGTTGAG	CGCC	0.2448	105	387	-	446	1	108145991
+Gnai3	CRISPRko	GAGTTCCTTGAAGGTGAAGTGGG	AGTA	0.3952	242	388	+	379	5	108115792
+Gnai3	CRISPRko	TAAAGTAGTTGTCTACAGCAATA	AGTA	0.4052	266	389	-	367	3	108123618
+Gnai3	CRISPRko	CTCCCTCAAAACAGTGAATCCAC	GTCA	0.4018	267	390	+	369	6	108112562
+Gnai3	CRISPRko	AAAAATGTGCAGTTTGTTTTTGA	CACC	0.4644	384	391	-	311	8	108109479
+Gnai3	CRISPRko	TCTTGCTCCGCTCCACCGCCGCC	ATCA	0.4351	318	392	+	344	1	108145975
+Gnai3	CRISPRko	TCCTCAGCCAGAACAAGGTCGTA	CTCA	0.2759	139	393	+	434	6	108112504
+Gnai3	CRISPRko	CACATTCCTTTAAGTTGTTTTTA	AGCC	0.4157	294	394	+	358	8	108109454
+Gnai3	CRISPRko	GGCATTGTGGAGACCCACTTCAC	TACA	0.3157	166	395	-	422	5	108115786
+Gnai3	CRISPRko	TGAAAGCATGAAATTGTTTGACA	TGCA	0.4518	375	396	-	318	7	108112057
+Gnai3	CRISPRko	GAATGCATGAAAGCATGAAATTG	AACC	0.5004	446	397	-	285	7	108112064
+Gnai3	CRISPRko	GGCATGCTTTAGCAGGTCCAGGG	TACA	0.3991	272	398	-	372	4	108118331
+Gnai3	CRISPRko	CTTACTGCTCTGGCAGATTCCCC	AATA	0.4085	291	399	+	364	3	108123558
+Gnai3	CRISPRko	AGAAAGACCTTTTTGAGGAAAAA	AATA	0.3914	257	400	-	383	7	108111980
+Gnai3	CRISPRko	CAGCCCCTCACCGAGCAGCAGCA	CCCC	0.4779	423	401	+	301	1	108145898
+Gnai3	CRISPRko	AAGGTGAAGTGGGTCTCCACAAT	CTTG	0.4675	418	402	+	308	5	108115802
+Gnai3	CRISPRko	TTGCTGTAGACAACTACTTTATA	AGTA	0.4242	337	403	+	352	3	108123639
+Gnai3	CRISPRko	GCCATGGGCTGCACGTTGAGCGC	CGCC	0.2611	158	404	-	443	1	108145988
+Gnai3	CRISPRko	TCATTAAAAACAACTTAAAGGAA	GTCA	0.4483	402	405	-	322	8	108109439
+Gnai3	CRISPRko	CAGTTTGTTTTTGATGCTGTTAC	TGTG	0.4682	435	406	-	307	8	108109470
+Gnai3	CRISPRko	ATAACCATGTTTGTTTTTCAGAA	TGTC	0.4263	349	407	-	351	7	108112089
+Gnai3	CRISPRko	TTTTTGAAGAATCATTCATGAGG	CTCC	0.4398	372	408	-	340	3	108123672
+Gnai3	CRISPRko	TGAACTTACTTCATCTGTTTCAC	TGTG	0.316	215	409	+	421	2	108123807
+Gnai3	CRISPRko	GCTCCGCCCCCAGCCCCTCACCG	TCCA	0.4445	397	410	+	330	1	108145888
+Gnai3	CRISPRko	CTTCACCTGTGCCACAGACACCA	CTCA	0.4208	348	411	-	356	8	108109501
+Gnai3	CRISPRko	ACATACCATTTCCTCATCCTCAG	GTCA	0.3224	230	412	+	419	6	108112488
+Gnai3	CRISPRko	AGAACAAGGTCGTAATCACTGAG	AGCC	0.3912	304	413	+	384	6	108112513
+Gnai3	CRISPRko	TGGAGACCCACTTCACCTTCAAG	ATTG	0.4465	419	414	-	327	5	108115779
+Gnai3	CRISPRko	GCACAGGTGAAGTGAGTGTAGAC	TGTG	0.4023	338	415	+	368	8	108109531
+Gnai3	CRISPRko	TATTCTGGATAACAGATTGTTAA	TGTG	0.3747	292	416	+	392	7	108111961
+Gnai3	CRISPRko	TCCTCGGCGCTCAACGTGCAGCC	CTTG	0.4423	407	417	+	335	1	108146001
+Gnai3	CRISPRko	AGCGCCGAGGACAAGGCGGCGGT	GTTG	0.4481	437	418	-	323	1	108145970
+Gnai3	CRISPRko	TTGGCCGCTTTCTCCCCGTCCTC	TTCT	0.3561	277	419	+	405	1	108145932
+Gnai3	CRISPRko	ATCTCGCCATAAACGTTTAATCA	CGCC	0.3198	260	420	+	420	4	108118384
+Gnai3	CRISPRko	TTCCAACTCAGCAAGATGTTCTT	TACA	0.2181	205	421	-	451	5	108115830
+Gnai3	CRISPRko	TTCATGAGGACGGCTATTCAGAG	ATCA	0.3363	286	422	-	411	3	108123658
+Gnai3	CRISPRko	TTCTCTTTCTTAATAAGAAAGAC	ATCA	0.3599	309	423	-	400	7	108111995
+Gnai3	CRISPRko	TAACCATGTTTGTTTTTCAGAAC	GTCA	0.3626	314	424	-	398	7	108112088
+Gnai3	CRISPRko	TGCATTTTATTTAGCTACCTAAA	AATA	0.274	247	425	-	435	5	108115885
+Gnai3	CRISPRko	CCCAGCCCCTCACCGAGCAGCAG	CGCC	0.2331	226	426	+	448	1	108145896
+Gnai3	CRISPRko	TTGAGCTGATATTCCCTGGACCT	ATCA	0.3942	359	427	+	382	4	108118337
+Gnai3	CRISPRko	TTTTATTTAGCTACCTAAATGAT	TGCA	0.3604	331	428	-	399	5	108115881
+Gnai3	CRISPRko	AAAAGGTCTTTCTTATTAAGAAA	CTCA	0.3581	332	429	+	401	7	108112009
+Gnai3	CRISPRko	TTCTGGCTGAGGATGAGGAAATG	CTTG	0.3993	393	430	-	371	6	108112475
+Gnai3	CRISPRko	AACAATTTCATGCTTTCATGCAT	GTCA	0.3338	308	431	+	414	7	108112081
+Gnai3	CRISPRko	ATGTTTGTTTTTCAGAACCGAAT	AACC	0.4006	396	432	-	370	7	108112083
+Gnai3	CRISPRko	TTTTTTTTCCCCTTACTCAGAAT	TTTT	0.3988	392	433	-	374	6	108112602
+Gnai3	CRISPRko	AAACAATTTCATGCTTTCATGCA	TGTC	0.3744	355	434	+	393	7	108112080
+Gnai3	CRISPRko	ATTCTCTTTCTTAATAAGAAAGA	AATC	0.3409	333	435	-	408	7	108111996
+Gnai3	CRISPRko	TGTCTTAACTGTTTTTCTCAGGA	CCCC	0.3893	379	436	-	388	4	108118459
+Gnai3	CRISPRko	TTCAACCGTCCCATGGCTCGTAT	AATC	0.2511	276	437	+	445	3	108123591
+Gnai3	CRISPRko	AACCATTTGTTGTTACAAATGCT	TGTA	0.313	322	438	+	423	7	108112054
+Gnai3	CRISPRko	AGCCCCTCACCGAGCAGCAGCAG	CCCC	0.2727	316	439	+	436	1	108145899
+Gnai3	CRISPRko	ATCTTCAACCGTCCCATGGCTCG	ATCA	0.39	426	440	+	387	3	108123588
+Gnai3	CRISPRko	TTAACTGTTTTTCTCAGGATGAT	TGTC	0.3569	395	441	-	403	4	108118455
+Gnai3	CRISPRko	TTAAAAACAACTTAAAGGAATGT	ATCA	0.3402	386	442	-	409	8	108109436
+Gnai3	CRISPRko	TTCAGTGCCAGTTTGAAGATCTG	TACA	0.357	415	443	-	402	8	108109559
+Gnai3	CRISPRko	GTTTGTTTTTGATGCTGTTACGG	TGCA	0.3832	443	444	-	389	8	108109468
+Gnai3	CRISPRko	AAGCCCACATTCCTTTAAGTTGT	AATA	0.2968	373	445	+	426	8	108109449
+Gnai3	CRISPRko	CATTTTTGGTGTCTGTGGCACAG	TGCA	0.2604	342	446	+	444	8	108109514
+Gnai3	CRISPRko	AAACAGTGAATCCACTTTTTTCG	CTCA	0.3009	380	447	+	425	6	108112570
+Gnai3	CRISPRko	CAATGCCTGTAGTCTTCACTCTT	TCCA	0.3225	404	448	+	418	5	108115821
+Gnai3	CRISPRko	AAAACAAACTGCACATTTTTGGT	ATCA	0.2803	390	449	+	431	8	108109501
+Gnai3	CRISPRko	CCACAGACACCAAAAATGTGCAG	TGTG	0.3245	429	450	-	416	8	108109490
+Gnai3	CRISPRko	GCCTTGTCCTCGGCGCTCAACGT	CGCC	0.2847	427	451	+	429	1	108145995
+Gnai3	CRISPRko	CTCACTTCACCTGTGCCACAGAC	TACA	0.2693	413	452	-	440	8	108109505
+Pbsn	CRISPRko	CTTAGCTGCCAGTACCATGGAAA	TTTA	0.9154	33	1	-	6	2	77848068
+Pbsn	CRISPRko	AGACTCATCACACTGGAGACGCC	TTTG	0.8523	27	2	+	12	1	77853440
+Pbsn	CRISPRko	AGATTGACGGGCCTTGGCAAACA	TTCC	0.7778	1	3	-	32	2	77848096
+Pbsn	CRISPRko	CAGATTGACGGGCCTTGGCAAAC	TTTC	0.8615	59	4	-	10	2	77848097
+Pbsn	CRISPRko	AGGAAAGGGACTAAGTGCCAACT	TTTC	1.004	85	5	-	1	3	77845068
+Pbsn	CRISPRko	ACATGAATGCTATGCTCCCTTCA	TTTA	0.8333	63	6	+	15	4	77843005
+Pbsn	CRISPRko	CCAGATTGACGGGCCTTGGCAAA	TTTT	0.7319	4	7	-	46	2	77848098
+Pbsn	CRISPRko	GAGACTCATCACACTGGAGACGC	TTTT	0.7467	18	8	+	41	1	77853439
+Pbsn	CRISPRko	ATGGTACTGGCAGCTAAGTAAAT	TTCC	0.9311	94	9	+	4	2	77848092
+Pbsn	CRISPRko	CGTGTCCATGATACGCTGTACAT	TACC	0.7129	3	10	+	52	5	77842537
+Pbsn	CRISPRko	TATGTGTGACACTTACCCAAAAC	GTTC	0.8636	93	11	+	9	4	77842902
+Pbsn	CRISPRko	CCTATACACCGTGAAACGCAGCA	ATTC	0.7933	54	12	-	29	5	77842617
+Pbsn	CRISPRko	TGTGGGAGGAGATGCAACCAAGT	TTTG	0.9047	98	13	-	7	2	77847998
+Pbsn	CRISPRko	CTCACACTGTGCATAGTAAACTT	CTTA	0.7514	40	14	+	37	3	77845035
+Pbsn	CRISPRko	CTATACACCGTGAAACGCAGCAA	TTCC	0.7886	55	15	-	30	5	77842616
+Pbsn	CRISPRko	ACAAGAACAGAAGAAATGATGTC	TTTA	0.9794	118	16	-	2	4	77842921
+Pbsn	CRISPRko	CCTTCCTATGACTTTATACAGTT	TTTT	0.7505	48	17	+	38	3	77845068
+Pbsn	CRISPRko	CCTATCTCAGATGAAGGGAGCAT	CTTA	0.7682	62	18	-	35	4	77842997
+Pbsn	CRISPRko	ACGGGCCTTGGCAAACAATTTAC	ATTG	0.6765	5	19	-	65	2	77848090
+Pbsn	CRISPRko	TCCAGATTGACGGGCCTTGGCAA	TTTT	0.6665	2	20	-	68	2	77848099
+Pbsn	CRISPRko	TCATTTACCATCTTTAACATGAA	CTTC	0.7394	53	21	+	43	4	77842989
+Pbsn	CRISPRko	GTGTGGGAGGAGATGCAACCAAG	TTTT	0.7975	83	22	-	28	2	77847999
+Pbsn	CRISPRko	GTGTTTACTCTCCTTTAGACAAC	TTCC	0.8257	104	23	-	18	6	77841909
+Pbsn	CRISPRko	GGCATTGCTTACTCACACTGTGC	TTCC	0.6836	17	24	+	62	3	77845024
+Pbsn	CRISPRko	TATACACCGTGAAACGCAGCAAA	TCCC	0.6586	8	25	-	70	5	77842615
+Pbsn	CRISPRko	GTTGCATCTCCTCCCACACAAAA	CTTG	0.6919	38	26	+	57	2	77848022
+Pbsn	CRISPRko	GTGGAAGAAATGGGCATTGAGGA	CTTA	0.8117	109	27	-	23	5	77842546
+Pbsn	CRISPRko	CTTCCTATGACTTTATACAGTTG	TTTC	0.8667	142	28	+	8	3	77845069
+Pbsn	CRISPRko	CACTAAGTTCATGAACTCCGTCA	CTTC	0.6596	23	29	+	69	5	77842585
+Pbsn	CRISPRko	CAGGAAAGGGACTAAGTGCCAAC	TTTT	0.8428	141	30	-	13	3	77845069
+Pbsn	CRISPRko	CATGGTACTGGCAGCTAAGTAAA	TTTC	0.9448	166	31	+	3	2	77848091
+Pbsn	CRISPRko	GGAAAGGGACTAAGTGCCAACTG	TTCA	0.7767	114	32	-	33	3	77845067
+Pbsn	CRISPRko	CCATCTTTAACATGAATGCTATG	TTTA	0.8378	158	33	+	14	4	77842996
+Pbsn	CRISPRko	TTCCACTAAGTTCATGAACTCCG	TTTC	0.7876	128	34	+	31	5	77842582
+Pbsn	CRISPRko	TTCTGTTCTTGTTAAAATAATGA	TTTC	0.8263	157	35	+	17	4	77842951
+Pbsn	CRISPRko	AGTGTGATGAGTCTCAAAAAGAA	CTCC	0.7221	95	36	-	48	1	77853412
+Pbsn	CRISPRko	TATCTCAGATGAAGGGAGCATAG	TACC	0.6225	35	37	-	79	4	77842995
+Pbsn	CRISPRko	ATGAACTCCGTCATCTCCTCCTT	GTTC	0.68	67	38	+	64	5	77842595
+Pbsn	CRISPRko	GCTGCCAGTACCATGGAAAAGAT	CTTA	0.8277	164	39	-	16	2	77848064
+Pbsn	CRISPRko	ATGATACGCTGTACATTCTCTTC	GTCC	0.6104	32	40	+	83	5	77842544
+Pbsn	CRISPRko	ATGTGTGACACTTACCCAAAACT	TTCT	0.621	39	41	+	80	4	77842903
+Pbsn	CRISPRko	TCAGGAAAGGGACTAAGTGCCAA	TTTT	0.8111	154	42	-	24	3	77845070
+Pbsn	CRISPRko	ACACGAGTGGCTGGAGTTTTGGG	TGTC	0.5805	16	43	-	98	4	77842898
+Pbsn	CRISPRko	CCCAAAACTCCAGCCACTCGTGT	CTTA	0.7503	134	44	+	39	4	77842917
+Pbsn	CRISPRko	TCAATGCCCATTTCTTCCACTAA	TTCC	0.7091	105	45	+	54	5	77842568
+Pbsn	CRISPRko	ATTGCTGCTTTTTTTCCAGATTG	TTCA	0.7162	111	46	-	51	2	77848113
+Pbsn	CRISPRko	ACTAAGTTCATGAACTCCGTCAT	TTCC	0.6857	92	47	+	61	5	77842586
+Pbsn	CRISPRko	TTCCAGATTGACGGGCCTTGGCA	TTTT	0.5775	15	48	-	101	2	77848100
+Pbsn	CRISPRko	GATGAAGGGAGCATAGCATTCAT	CTCA	0.5635	6	49	-	107	4	77842988
+Pbsn	CRISPRko	CTACTATACCCGTGTCCATGATA	GTTG	0.6513	76	50	+	73	5	77842527
+Pbsn	CRISPRko	TACAGTTGGCACTTAGTCCCTTT	TTTA	0.7214	124	51	+	49	3	77845084
+Pbsn	CRISPRko	TGGGAGGAGATGCAACCAAGTCT	TGTG	0.5951	41	52	-	91	2	77847996
+Pbsn	CRISPRko	ATTATTTTAACAAGAACAGAAGA	TTTC	0.8606	203	53	-	11	4	77842930
+Pbsn	CRISPRko	GGAGGAGATGCAACCAAGTCTAC	TGTG	0.652	82	54	-	72	2	77847994
+Pbsn	CRISPRko	ATTGAGAACCTACTTCCGTCACA	CACC	0.5905	45	55	-	93	2	77848026
+Pbsn	CRISPRko	CTAGGCGTCTCCAGTGTGATGAG	TGTG	0.5572	13	56	-	109	1	77853424
+Pbsn	CRISPRko	GTCACATTTTGTGTGGGAGGAGA	TTCC	0.6091	65	57	-	84	2	77848009
+Pbsn	CRISPRko	TCTTTTCCATGGTACTGGCAGCT	TTTA	0.8104	183	58	+	25	2	77848084
+Pbsn	CRISPRko	ATGGAAAAGATAAATGAAGGCTC	TACC	0.7461	151	59	-	42	2	77848052
+Pbsn	CRISPRko	CGTGTTTACTCTCCTTTAGACAA	TTTC	0.9171	226	60	-	5	6	77841910
+Pbsn	CRISPRko	GAGGTAGACTTGGTTGCATCTCC	AATA	0.557	19	61	+	110	2	77848010
+Pbsn	CRISPRko	AAGATGGTAAATGAGAAGATATT	GTTA	0.8212	202	62	-	19	4	77842961
+Pbsn	CRISPRko	TCTGTTCTTGTTAAAATAATGAA	TTCT	0.6758	110	63	+	66	4	77842952
+Pbsn	CRISPRko	CCATGGTACTGGCAGCTAAGTAA	TTTT	0.5995	66	64	+	88	2	77848090
+Pbsn	CRISPRko	TTTTCCATGGTACTGGCAGCTAA	TATC	0.5825	51	65	+	96	2	77848086
+Pbsn	CRISPRko	CAAATGTCTTACCTATCTCAGAT	TATA	0.603	74	66	-	85	4	77843008
+Pbsn	CRISPRko	CATCTCCTCCCACACAAAATGTG	GTTG	0.6002	73	67	+	87	2	77848026
+Pbsn	CRISPRko	CACGTAGGTTGCTACTATACCCG	TACA	0.5308	14	68	+	118	5	77842516
+Pbsn	CRISPRko	ACGGAAGTAGGTTCTCAATGGTG	TGTG	0.5276	11	69	+	120	2	77848049
+Pbsn	CRISPRko	CTCAATGCCCATTTCTTCCACTA	CTTC	0.5991	72	70	+	90	5	77842567
+Pbsn	CRISPRko	TCAATGGTGAGCCTTCATTTATC	GTTC	0.6801	127	71	+	63	2	77848063
+Pbsn	CRISPRko	GTCTATATATATCTTACTTAATA	TTCT	0.5749	50	72	+	102	2	77847979
+Pbsn	CRISPRko	ATAAAAAAATAGAGGTAGACTTG	CTTA	0.8015	201	73	+	27	2	77847999
+Pbsn	CRISPRko	AAATAATGAAACAACAATATCTT	GTTA	0.8179	214	74	+	21	4	77842965
+Pbsn	CRISPRko	CTTAATAAAAAAATAGAGGTAGA	CTTA	0.7763	189	75	+	34	2	77847995
+Pbsn	CRISPRko	TTCCTATGACTTTATACAGTTGG	TTCC	0.7338	171	76	+	44	3	77845070
+Pbsn	CRISPRko	ACACAGGTCTGCTACCTTCTTTT	TTCC	0.6139	100	77	+	82	1	77853415
+Pbsn	CRISPRko	TATGACTTTATACAGTTGGCACT	TTCC	0.7101	161	78	+	53	3	77845074
+Pbsn	CRISPRko	CTCTCCTTTAGACAACTGTCCAA	TTTA	0.8124	224	79	-	22	6	77841902
+Pbsn	CRISPRko	CAAAACTCCAGCCACTCGTGTGA	TACC	0.5687	60	80	+	104	4	77842919
+Pbsn	CRISPRko	AATTGCTGCTTTTTTTCCAGATT	TTTC	0.8185	229	81	-	20	2	77848114
+Pbsn	CRISPRko	TGTTCTTGTTAAAATAATGAAAC	CTTC	0.7474	190	82	+	40	4	77842954
+Pbsn	CRISPRko	TGTCTATATATATCTTACTTAAT	GTTC	0.7606	200	83	+	36	2	77847978
+Pbsn	CRISPRko	TCCCACACAAAATGTGACGGAAG	CTCC	0.5233	28	84	+	123	2	77848033
+Pbsn	CRISPRko	GACAACTGTCCAAGCAAGATCAA	TTTA	0.8091	225	85	-	26	6	77841892
+Pbsn	CRISPRko	ATCTTTAACATGAATGCTATGCT	TACC	0.5425	52	86	+	113	4	77842998
+Pbsn	CRISPRko	TATCTTACTTAATAAAAAAATAG	TATA	0.6025	106	87	+	86	2	77847988
+Pbsn	CRISPRko	CGTCACATTTTGTGTGGGAGGAG	CTTC	0.652	136	88	-	71	2	77848010
+Pbsn	CRISPRko	TCCTTCCTATGACTTTATACAGT	TTTT	0.7224	185	89	+	47	3	77845067
+Pbsn	CRISPRko	AACAAGAACAGAAGAAATGATGT	TTTT	0.717	180	90	-	50	4	77842922
+Pbsn	CRISPRko	TTTTTCAGGAAAGGGACTAAGTG	TTTG	0.7321	192	91	-	45	3	77845074
+Pbsn	CRISPRko	CCCGTGTCCATGATACGCTGTAC	TATA	0.5008	7	92	+	139	5	77842535
+Pbsn	CRISPRko	CAGTTGGCACTTAGTCCCTTTCC	TATA	0.5993	108	93	+	89	3	77845086
+Pbsn	CRISPRko	ATGGTGAGCCTTCATTTATCTTT	CTCA	0.5282	49	94	+	119	2	77848066
+Pbsn	CRISPRko	TTACCTATCTCAGATGAAGGGAG	TGTC	0.5687	81	95	-	105	4	77843000
+Pbsn	CRISPRko	GTGTGAGCAGGAGGATGATGACC	TGCA	0.5144	31	96	+	131	1	77853474
+Pbsn	CRISPRko	TTCCTTCCTATGACTTTATACAG	TTTT	0.687	176	97	+	59	3	77845066
+Pbsn	CRISPRko	TCCACTAAGTTCATGAACTCCGT	TTCT	0.5656	84	98	+	106	5	77842583
+Pbsn	CRISPRko	TTGTTAAAATAATGAAACAACAA	GTTC	0.7075	191	99	+	55	4	77842959
+Pbsn	CRISPRko	TGTTAAAATAATGAAACAACAAT	TTCT	0.6886	187	100	+	58	4	77842960
+Pbsn	CRISPRko	AGTCACTAAGGTTTGATCTTGCT	GTTG	0.5336	79	101	+	117	6	77841899
+Pbsn	CRISPRko	TGAACTCCGTCATCTCCTCCTTA	TTCA	0.6237	160	102	+	78	5	77842596
+Pbsn	CRISPRko	CATGATACGCTGTACATTCTCTT	TGTC	0.6398	168	103	+	76	5	77842543
+Pbsn	CRISPRko	TTATTTTAACAAGAACAGAAGAA	TTCA	0.6954	209	104	-	56	4	77842929
+Pbsn	CRISPRko	CAATTGCTGCTTTTTTTCCAGAT	TTTT	0.6869	205	105	-	60	2	77848115
+Pbsn	CRISPRko	AGCCACTCGTGTGACATCATTTC	CTCC	0.5227	78	106	+	124	4	77842928
+Pbsn	CRISPRko	CAATGGTGAGCCTTCATTTATCT	TTCT	0.5928	144	107	+	92	2	77848064
+Pbsn	CRISPRko	ATGAACTTAGTGGAAGAAATGGG	GTTC	0.6401	181	108	-	75	5	77842555
+Pbsn	CRISPRko	CCATTGAGAACCTACTTCCGTCA	CTCA	0.4903	37	109	-	147	2	77848028
+Pbsn	CRISPRko	AGCAGGAGGATGATGACCCTCAT	TGTG	0.5169	75	110	+	129	1	77853479
+Pbsn	CRISPRko	CTATGACTTTATACAGTTGGCAC	CTTC	0.5814	140	111	+	97	3	77845073
+Pbsn	CRISPRko	TGTTAAAGATGGTAAATGAGAAG	TTCA	0.6672	207	112	-	67	4	77842966
+Pbsn	CRISPRko	CATTTCTTCCACTAAGTTCATGA	TGCC	0.4775	42	113	+	154	5	77842576
+Pbsn	CRISPRko	TTGAGTCACTAAGGTTTGATCTT	CTTG	0.5147	91	114	+	130	6	77841896
+Pbsn	CRISPRko	TGAACTTAGTGGAAGAAATGGGC	TTCA	0.5826	162	115	-	95	5	77842554
+Pbsn	CRISPRko	TAGCACATGCAGTGTGAGCAGGA	CGCC	0.4551	26	116	+	163	1	77853463
+Pbsn	CRISPRko	ATTCCGGCATTGCTTACTCACAC	ATTC	0.4848	57	117	+	148	3	77845019
+Pbsn	CRISPRko	GATTGACGGGCCTTGGCAAACAA	TCCA	0.5342	121	118	-	116	2	77848095
+Pbsn	CRISPRko	TTTCCAGATTGACGGGCCTTGGC	TTTT	0.4439	9	119	-	173	2	77848101
+Pbsn	CRISPRko	GTGTCCATGATACGCTGTACATT	ACCC	0.4827	56	120	+	150	5	77842538
+Pbsn	CRISPRko	AGTTCTATGTGTGACACTTACCC	TACA	0.5111	88	121	+	134	4	77842897
+Pbsn	CRISPRko	AAAACTCCAGCCACTCGTGTGAC	ACCC	0.4443	12	122	+	172	4	77842920
+Pbsn	CRISPRko	ATGTTAAAGATGGTAAATGAGAA	ATTC	0.6161	195	123	-	81	4	77842967
+Pbsn	CRISPRko	CACGAGTGGCTGGAGTTTTGGGT	GTCA	0.4517	24	124	-	168	4	77842897
+Pbsn	CRISPRko	CTTACTCACACTGTGCATAGTAA	ATTG	0.4592	44	125	+	160	3	77845031
+Pbsn	CRISPRko	CCGTGAAACGCAGCAAAAGGCAA	TACA	0.52	113	126	-	127	5	77842609
+Pbsn	CRISPRko	ATAGGAAGGAAAAAACAAGAAGT	AGTC	0.6396	213	127	-	77	3	77845035
+Pbsn	CRISPRko	AAGTCATAGGAAGGAAAAAACAA	TATA	0.6423	220	128	-	74	3	77845040
+Pbsn	CRISPRko	CGGCATTGCTTACTCACACTGTG	ATTC	0.4452	30	129	+	171	3	77845023
+Pbsn	CRISPRko	TCAGATGAAGGGAGCATAGCATT	TATC	0.4523	43	130	-	166	4	77842991
+Pbsn	CRISPRko	TTGTTTTTTCCTTCCTATGACTT	CTTC	0.5392	148	131	+	114	3	77845059
+Pbsn	CRISPRko	AGGAAGAGAATGTACAGCGTATC	ATTG	0.4716	64	132	-	157	5	77842527
+Pbsn	CRISPRko	ACACAAAATGTGACGGAAGTAGG	TCCC	0.5235	137	133	+	121	2	77848037
+Pbsn	CRISPRko	TGACACTTACCCAAAACTCCAGC	TGTG	0.4366	25	134	+	178	4	77842908
+Pbsn	CRISPRko	TGGTACTGGCAGCTAAGTAAATT	TCCA	0.5112	115	135	+	133	2	77848093
+Pbsn	CRISPRko	TGAGACTCATCACACTGGAGACG	TTTT	0.5902	194	136	+	94	1	77853438
+Pbsn	CRISPRko	CTAAGTTCATGAACTCCGTCATC	TCCA	0.4401	36	137	+	174	5	77842587
+Pbsn	CRISPRko	ATTTATCTTTTCCATGGTACTGG	CTTC	0.5143	120	138	+	132	2	77848079
+Pbsn	CRISPRko	TTTAGACAACTGTCCAAGCAAGA	CTCC	0.4971	103	139	-	141	6	77841896
+Pbsn	CRISPRko	CACCGTGAAACGCAGCAAAAGGC	TATA	0.4141	21	140	-	186	5	77842611
+Pbsn	CRISPRko	ACACTTACCCAAAACTCCAGCCA	TGTG	0.4652	77	141	+	158	4	77842910
+Pbsn	CRISPRko	TTGTTTCATTATTTTAACAAGAA	ATTG	0.5778	193	142	-	100	4	77842937
+Pbsn	CRISPRko	TCACACTGGAGACGCCTAGCACA	CTCA	0.4462	58	143	+	170	1	77853447
+Pbsn	CRISPRko	TTTATCTTTTCCATGGTACTGGC	TTCA	0.4973	119	144	+	140	2	77848080
+Pbsn	CRISPRko	AATTGTTTGCCAAGGCCCGTCAA	AGTA	0.4051	20	145	+	191	2	77848112
+Pbsn	CRISPRko	TTAAAATAATGAAACAACAATAT	CTTG	0.5794	206	146	+	99	4	77842962
+Pbsn	CRISPRko	AGTACCATGGAAAAGATAAATGA	TGCC	0.5216	155	147	-	125	2	77848058
+Pbsn	CRISPRko	TGATCTTGTTGAGTCACTAAGGT	ATCC	0.4928	117	148	+	145	6	77841888
+Pbsn	CRISPRko	ACTTTATACAGTTGGCACTTAGT	TATG	0.4078	29	149	+	190	3	77845078
+Pbsn	CRISPRko	AACTGTATAAAGTCATAGGAAGG	TGCC	0.5078	139	150	-	137	3	77845049
+Pbsn	CRISPRko	TTTTTTCCTTCCTATGACTTTAT	CTTG	0.5688	208	151	+	103	3	77845062
+Pbsn	CRISPRko	GTGAAACGCAGCAAAAGGCAAAC	CACC	0.5181	159	152	-	128	5	77842607
+Pbsn	CRISPRko	TCCTTATTCAGTTGTTTGCCTTT	CTCC	0.4582	97	153	+	161	5	77842613
+Pbsn	CRISPRko	CACACAAAATGTGACGGAAGTAG	CTCC	0.3826	22	154	+	200	2	77848036
+Pbsn	CRISPRko	GCAAACAATTTACTTAGCTGCCA	CTTG	0.4377	69	155	-	177	2	77848080
+Pbsn	CRISPRko	ATACGACATTTGTTTTTCAGGAA	TACA	0.4906	132	156	-	146	3	77845086
+Pbsn	CRISPRko	TCTCCTCCCACACAAAATGTGAC	TGCA	0.5211	172	157	+	126	2	77848028
+Pbsn	CRISPRko	TCTTACTTAATAAAAAAATAGAG	TATA	0.5584	210	158	+	108	2	77847990
+Pbsn	CRISPRko	TAGTAAACTTCTTGTTTTTTCCT	TGCA	0.4967	143	159	+	142	3	77845048
+Pbsn	CRISPRko	GCTCACACATGATGAGGGTCATC	AGTC	0.3428	10	160	-	209	1	77853471
+Pbsn	CRISPRko	TTCCGGCATTGCTTACTCACACT	TTCA	0.4773	122	161	+	155	3	77845020
+Pbsn	CRISPRko	TTTCATTATTTTAACAAGAACAG	GTTG	0.5447	212	162	-	112	4	77842934
+Pbsn	CRISPRko	TTCTGTCTATATATATCTTACTT	ATTG	0.5111	175	163	+	135	2	77847975
+Pbsn	CRISPRko	CTAAGGTTTGATCTTGCTTGGAC	GTCA	0.3795	47	164	+	201	6	77841904
+Pbsn	CRISPRko	ACTCGTGTGACATCATTTCTTCT	AGCC	0.3459	34	165	+	208	4	77842932
+Pbsn	CRISPRko	TTACTTAATAAAAAAATAGAGGT	TATC	0.5471	228	166	+	111	2	77847992
+Pbsn	CRISPRko	TAAAGTCATAGGAAGGAAAAAAC	TGTA	0.4781	145	167	-	153	3	77845042
+Pbsn	CRISPRko	CACTGCATGTGCTAGGCGTCTCC	CTCA	0.4387	101	168	-	175	1	77853435
+Pbsn	CRISPRko	TGATACGCTGTACATTCTCTTCC	TCCA	0.494	167	169	+	144	5	77842545
+Pbsn	CRISPRko	AATGTCTTACCTATCTCAGATGA	TACA	0.5345	227	170	-	115	4	77843006
+Pbsn	CRISPRko	AGGAGGAGATGACGGAGTTCATG	AATA	0.4771	147	171	-	156	5	77842575
+Pbsn	CRISPRko	TTTTAACAAGAACAGAAGAAATG	ATTA	0.5235	215	172	-	122	4	77842926
+Pbsn	CRISPRko	GTTCTTGTTAAAATAATGAAACA	TTCT	0.4536	131	173	+	164	4	77842955
+Pbsn	CRISPRko	CACACAGGTCTGCTACCTTCTTT	CTTC	0.4952	174	174	+	143	1	77853414
+Pbsn	CRISPRko	CATAGTAAACTTCTTGTTTTTTC	TGTG	0.4123	89	175	+	187	3	77845046
+Pbsn	CRISPRko	GTCATCTCCTCCTTATTCAGTTG	CTCC	0.3903	70	176	+	197	5	77842604
+Pbsn	CRISPRko	TGTGACACTTACCCAAAACTCCA	TATG	0.4481	126	177	+	169	4	77842906
+Pbsn	CRISPRko	CTTCCTCAATGCCCATTTCTTCC	TTCT	0.4803	163	178	+	152	5	77842563
+Pbsn	CRISPRko	ATGAAACAACAATATCTTCTCAT	AATA	0.4828	169	179	+	149	4	77842970
+Pbsn	CRISPRko	GCAAAAGGCAAACAACTGAATAA	CGCA	0.4627	150	180	-	159	5	77842597
+Pbsn	CRISPRko	CATTTACCATCTTTAACATGAAT	TTCT	0.4563	146	181	+	162	4	77842990
+Pbsn	CRISPRko	TCCTGCTCACACTGCATGTGCTA	ATCC	0.372	68	182	-	202	1	77853444
+Pbsn	CRISPRko	TTCTCATTTACCATCTTTAACAT	TATC	0.5107	204	183	+	136	4	77842986
+Pbsn	CRISPRko	AGAACCTACTTCCGTCACATTTT	ATTG	0.4114	99	184	-	189	2	77848022
+Pbsn	CRISPRko	TGCTCACACTGCATGTGCTAGGC	CTCC	0.3687	71	185	-	203	1	77853441
+Pbsn	CRISPRko	TCCAGTGTGATGAGTCTCAAAAA	CGTC	0.4518	152	186	-	167	1	77853415
+Pbsn	CRISPRko	TTTACCATCTTTAACATGAATGC	CTCA	0.436	129	187	+	180	4	77842992
+Pbsn	CRISPRko	GGTTCTCAATGGTGAGCCTTCAT	AGTA	0.4271	125	188	+	182	2	77848058
+Pbsn	CRISPRko	CACATGATGAGGGTCATCATCCT	CTCA	0.3875	102	189	-	198	1	77853466
+Pbsn	CRISPRko	ACTAAGGTTTGATCTTGCTTGGA	AGTC	0.3956	107	190	+	196	6	77841903
+Pbsn	CRISPRko	TAGGAAGGAAAAAACAAGAAGTT	GTCA	0.5043	223	191	-	138	3	77845034
+Pbsn	CRISPRko	CACTGGAGACGCCTAGCACATGC	ATCA	0.2941	61	192	+	219	1	77853450
+Pbsn	CRISPRko	CACTGTGCATAGTAAACTTCTTG	CTCA	0.4271	138	193	+	181	3	77845039
+Pbsn	CRISPRko	TGTGCTAGGCGTCTCCAGTGTGA	TGCA	0.3411	80	194	-	210	1	77853428
+Pbsn	CRISPRko	ATCTCCTCCTTATTCAGTTGTTT	CGTC	0.1235	46	195	+	229	5	77842607
+Pbsn	CRISPRko	GTGTGATGAGTCTCAAAAAGAAG	TCCA	0.4117	130	196	-	188	1	77853411
+Pbsn	CRISPRko	CTGCTTTTTTTCCAGATTGACGG	ATTG	0.436	149	197	-	179	2	77848109
+Pbsn	CRISPRko	CTGATCTTGTTGAGTCACTAAGG	AATC	0.3238	87	198	+	212	6	77841887
+Pbsn	CRISPRko	TATATATATCTTACTTAATAAAA	TGTC	0.4815	216	199	+	151	2	77847982
+Pbsn	CRISPRko	TACTTCCGTCACATTTTGTGTGG	AACC	0.3314	96	200	-	211	2	77848016
+Pbsn	CRISPRko	TTCTCTTCCTCAATGCCCATTTC	TACA	0.3114	90	201	+	215	5	77842559
+Pbsn	CRISPRko	CATTCTCTTCCTCAATGCCCATT	TGTA	0.397	133	202	+	194	5	77842557
+Pbsn	CRISPRko	ACATTTTGTGTGGGAGGAGATGC	CGTC	0.2813	86	203	-	221	2	77848006
+Pbsn	CRISPRko	CACAGGTCTGCTACCTTCTTTTT	TCCA	0.3832	135	204	+	199	1	77853416
+Pbsn	CRISPRko	ACCAAGTCTACCTCTATTTTTTT	TGCA	0.4271	173	205	-	183	2	77847982
+Pbsn	CRISPRko	ACATCATTTCTTCTGTTCTTGTT	TGTG	0.4146	170	206	+	185	4	77842941
+Pbsn	CRISPRko	TCTTCCTCAATGCCCATTTCTTC	ATTC	0.4204	179	207	+	184	5	77842562
+Pbsn	CRISPRko	CTTCCACACAGGTCTGCTACCTT	CCCC	0.4527	218	208	+	165	1	77853410
+Pbsn	CRISPRko	GCCACTCGTGTGACATCATTTCT	TCCA	0.3149	123	209	+	213	4	77842929
+Pbsn	CRISPRko	AAAAAATAGAGGTAGACTTGGTT	AATA	0.4047	165	210	+	192	2	77848002
+Pbsn	CRISPRko	TATATCTTACTTAATAAAAAAAT	TATA	0.4382	199	211	+	176	2	77847986
+Pbsn	CRISPRko	TCTCCTCCTTATTCAGTTGTTTG	GTCA	0.2773	116	212	+	222	5	77842608
+Pbsn	CRISPRko	CGACATTTGTTTTTCAGGAAAGG	AATA	0.2032	112	213	-	228	3	77845083
+Pbsn	CRISPRko	AAGTCTACCTCTATTTTTTTATT	AACC	0.4029	197	214	-	193	2	77847979
+Pbsn	CRISPRko	TTTTTGAGACTCATCACACTGGA	CTTC	0.3969	198	215	+	195	1	77853434
+Pbsn	CRISPRko	ATGCCCATTTCTTCCACTAAGTT	CTCA	0.2994	153	216	+	218	5	77842571
+Pbsn	CRISPRko	TCTTCTCATTTACCATCTTTAAC	AATA	0.3561	184	217	+	204	4	77842984
+Pbsn	CRISPRko	TGTTTTTTCCTTCCTATGACTTT	TTCT	0.355	188	218	+	205	3	77845060
+Pbsn	CRISPRko	TTTTGAGACTCATCACACTGGAG	TTCT	0.2605	156	219	+	223	1	77853435
+Pbsn	CRISPRko	TTATTCAGTTGTTTGCCTTTTGC	CTCC	0.3497	196	220	+	207	5	77842616
+Pbsn	CRISPRko	CCATGGAAAAGATAAATGAAGGC	AGTA	0.302	182	221	-	217	2	77848054
+Pbsn	CRISPRko	TCCTCCTGCTCACACTGCATGTG	ATCA	0.2535	178	222	-	224	1	77853447
+Pbsn	CRISPRko	TTCTTTTTGAGACTCATCACACT	TACC	0.2384	177	223	+	226	1	77853431
+Pbsn	CRISPRko	TCATCCTCCTGCTCACACTGCAT	GTCA	0.3504	217	224	-	206	1	77853450
+Pbsn	CRISPRko	CATTTTGTGTGGGAGGAGATGCA	GTCA	0.2244	186	225	-	227	2	77848005
+Pbsn	CRISPRko	TTTCTTCTGTTCTTGTTAAAATA	ATCA	0.3138	222	226	+	214	4	77842947
+Pbsn	CRISPRko	AACTTCTTGTTTTTTCCTTCCTA	AGTA	0.2939	211	227	+	220	3	77845053
+Pbsn	CRISPRko	TTCATTTATCTTTTCCATGGTAC	AGCC	0.3032	221	228	+	216	2	77848076
+Pbsn	CRISPRko	TTCCACACAGGTCTGCTACCTTC	CCCC	0.2446	219	229	+	225	1	77853411
+Cdc45	CRISPRko	GGTGGTTATGACGGGACACATGA	TTTC	0.9685	26	1	+	2	11	18795907
+Cdc45	CRISPRko	ACTACGAGCAGTATGAATATTAT	TTTG	0.8092	34	2	-	39	8	18801812
+Cdc45	CRISPRko	TAGGTATCCGCCGCTGTGGCTAT	GTTC	0.8141	40	3	-	37	2	18811566
+Cdc45	CRISPRko	GTCGGGCTTTTGAGAAGGCAGCA	TTTG	0.9097	98	4	-	12	18	18784854
+Cdc45	CRISPRko	ACGCTCAGCGGGGCAGCCATCAC	TTCC	0.7865	20	5	+	58	17	18785157
+Cdc45	CRISPRko	ACCTGGCGTGGATGATGTCCAAG	TTTG	0.8253	75	6	-	33	9	18798698
+Cdc45	CRISPRko	GTGTGACCACGTCCAGTATACGC	TTCA	0.7876	29	7	-	57	4	18810450
+Cdc45	CRISPRko	CCTTCAAGGAAACGTCCATGGAC	TTCC	0.8257	106	8	+	32	13	18794826
+Cdc45	CRISPRko	TGCTTGAACCCAAACTGAATGCT	TTTA	0.8885	139	9	+	17	14	18793310
+Cdc45	CRISPRko	TGGCCTCGGACGTGGATGCCTTG	TTTG	0.7533	30	10	-	76	3	18811328
+Cdc45	CRISPRko	ATACTTACCGATGTCCCATAATA	GTTC	0.7688	63	11	+	69	8	18801814
+Cdc45	CRISPRko	CGCAAGGAGTTCTACGAGACGGT	TTTC	0.8523	161	12	-	21	2	18811527
+Cdc45	CRISPRko	TCTGAAGGCTCTGACCCATCACC	CTTC	0.8261	152	13	+	31	6	18807323
+Cdc45	CRISPRko	ATCCTCATAAACTGTGGAGCCAA	TTTC	0.9003	203	14	-	13	5	18808837
+Cdc45	CRISPRko	GGTTCAAGCATAAATTCCTGGCC	TTTG	0.877	199	15	-	18	14	18793278
+Cdc45	CRISPRko	CACGCTCAGCGGGGCAGCCATCA	GTTC	0.7295	53	16	+	92	17	18785156
+Cdc45	CRISPRko	GTGACCGATTTCCGCAAGGAGTT	GTTC	0.6962	5	17	-	118	2	18811539
+Cdc45	CRISPRko	TAACAGATCAAATTGCTTATTAA	TTTA	0.9266	222	18	-	10	6	18807409
+Cdc45	CRISPRko	CTGGACGCTCTTGTGTCACTGCT	GTTC	0.7837	120	19	-	62	19	18781907
+Cdc45	CRISPRko	ATCCAGGCTCTCGATAGCCTCTC	CTTC	0.7085	31	20	-	109	14	18793183
+Cdc45	CRISPRko	ATCTCTAAAGATGTCGTCGTAGG	CTTC	0.7109	49	21	+	105	6	18807379
+Cdc45	CRISPRko	CAAGCCAGCATCCTTGAGTCTGC	TTTC	0.9385	249	22	-	6	16	18786806
+Cdc45	CRISPRko	CTGGCCAGTGATGTCGTCTTTGC	ATTC	0.8016	176	23	-	43	14	18793261
+Cdc45	CRISPRko	TAAGGTATTCAAAGCTCACCTGG	TTCC	0.7368	97	24	+	85	3	18811307
+Cdc45	CRISPRko	CTTGACCTTGGACCTAGCCTCTG	ATTC	0.7295	83	25	-	93	12	18795223
+Cdc45	CRISPRko	ATAGACAAAGAATCGACGATGCA	CTCC	0.6782	21	26	-	131	17	18785175
+Cdc45	CRISPRko	AACCTGGCTGCTGTGTAGCTGGT	CTTA	0.7533	134	27	+	77	12	18795173
+Cdc45	CRISPRko	CCACCATGTCTCTAATGGAGAGT	TTTG	0.7742	159	28	-	66	14	18793239
+Cdc45	CRISPRko	CCGATGTCCCATAATATTCATAC	CTTA	0.8	201	29	+	46	8	18801821
+Cdc45	CRISPRko	CAGGGCCCTTTTCTCTACTGCTC	TTCC	0.756	150	30	-	72	15	18786942
+Cdc45	CRISPRko	TACTTACCGATGTCCCATAATAT	TTCA	0.7032	77	31	+	111	8	18801815
+Cdc45	CRISPRko	GCTTTTTTGGTCGGGCTTTTGAG	TTTA	0.6894	57	32	-	123	18	18784863
+Cdc45	CRISPRko	TAACCGTGTGCGCTTCTCTGAAG	CTTC	0.7025	79	33	+	112	6	18807307
+Cdc45	CRISPRko	CCCTGAAGCAAGTCAAGCAGAAA	CTTC	0.7309	125	34	-	91	13	18794835
+Cdc45	CRISPRko	AGTCCATGGACGTTTCCTTGAAG	TTTC	0.928	293	35	-	8	13	18794808
+Cdc45	CRISPRko	ATAACAGATCAAATTGCTTATTA	TTTT	0.7512	151	36	-	79	6	18807410
+Cdc45	CRISPRko	TGACGGGACACATGACGCTGCAG	GTTA	0.7558	164	37	+	73	11	18795915
+Cdc45	CRISPRko	CCCCATGTCTGCAAGGAACTCCT	CTTA	0.745	147	38	+	82	12	18795115
+Cdc45	CRISPRko	TGGACGCTCTTGTGTCACTGCTG	TTCC	0.8128	237	39	-	38	19	18781906
+Cdc45	CRISPRko	TGGTCGGGCTTTTGAGAAGGCAG	TTTT	0.6994	84	40	-	115	18	18784856
+Cdc45	CRISPRko	GTGTTGTTACAGGAAAGACATCC	TTTT	0.7367	143	41	-	86	8	18801841
+Cdc45	CRISPRko	ATGGAGGGACCAGTGCTGATACA	TTTC	0.9275	298	42	+	9	12	18795211
+Cdc45	CRISPRko	AGTGTGACCACGTCCAGTATACG	TTTC	1.015	318	43	-	1	4	18810451
+Cdc45	CRISPRko	AGGAGTAACCTGGACAAGCTATA	TTTC	0.9664	320	44	-	4	15	18787050
+Cdc45	CRISPRko	AGGGCACTCCAGATGTCACCCTG	TTCC	0.8001	242	45	-	45	16	18786834
+Cdc45	CRISPRko	GTCGTAGGCAGGAACCTCAAGGT	TGTC	0.6634	50	46	+	141	6	18807394
+Cdc45	CRISPRko	TACGAGACGGTCCACAACCAGGT	GTTC	0.8163	262	47	-	36	2	18811515
+Cdc45	CRISPRko	GATGTCCCATAATATTCATACTG	TACC	0.7141	129	48	+	104	8	18801823
+Cdc45	CRISPRko	TCGCAGATTCCCCTTCAAGGAAA	TTTC	0.8296	283	49	+	28	13	18794815
+Cdc45	CRISPRko	CTTTCATTACTTCAGTAATTGAG	TTTC	0.8608	311	50	-	20	19	18781957
+Cdc45	CRISPRko	AAGGACCTGAATGACATGCTGTG	GTCC	0.6732	87	51	-	133	9	18798678
+Cdc45	CRISPRko	CAGAATGAAGTACGTGACTGATG	CTTC	0.7448	193	52	-	83	11	18795927
+Cdc45	CRISPRko	CTTGCAGTCGGCCATGGTGATGT	TTTC	0.8895	327	53	-	16	9	18798724
+Cdc45	CRISPRko	TGACCGATTTCCGCAAGGAGTTC	TTCG	0.6585	67	54	-	148	2	18811538
+Cdc45	CRISPRko	TATCCGAGCTATCAGTCTCTGGG	TTTC	0.7995	271	55	+	47	17	18785101
+Cdc45	CRISPRko	TTTGCAGATTCTTCAATCATTTC	TTTA	0.7948	260	56	+	54	13	18794792
+Cdc45	CRISPRko	GCAAGGAGTTCTACGAGACGGTC	TTCC	0.8327	323	57	-	26	2	18811526
+Cdc45	CRISPRko	GGTCCTTGGACATCATCCACGCC	TTCA	0.6664	102	58	+	139	9	18798713
+Cdc45	CRISPRko	GGAGTAACCTGGACAAGCTATAC	TTCA	0.7857	265	59	-	59	15	18787049
+Cdc45	CRISPRko	GAGAAGCGCACACGGTTAGAAGA	TTCA	0.6398	39	60	-	172	6	18807283
+Cdc45	CRISPRko	CTGGACGTGGTCACACTGAAACA	TATA	0.6688	110	61	+	137	4	18810476
+Cdc45	CRISPRko	ACAGATCAAATTGCTTATTAAAC	TATA	0.6832	142	62	-	126	6	18807407
+Cdc45	CRISPRko	CTGGCCTGTGGGTATCACACACA	TTCA	0.6826	140	63	+	128	5	18808785
+Cdc45	CRISPRko	GGGTGCAGTCTACAGAGAGCATG	ATCC	0.6445	70	64	+	166	11	18795865
+Cdc45	CRISPRko	TGCGCTACATACCACAGCATGTC	TATG	0.6271	25	65	+	189	9	18798685
+Cdc45	CRISPRko	CCCAAATTTATTTGCAGATTCTT	TTTA	0.8247	337	66	+	34	13	18794782
+Cdc45	CRISPRko	ATTGTACACATTCACAACGTTCA	TGTC	0.7162	209	67	+	103	5	18808762
+Cdc45	CRISPRko	ACGGGACACATGACGCTGCAGGA	TATG	0.6414	80	68	+	171	11	18795917
+Cdc45	CRISPRko	GTGGTTATGACGGGACACATGAC	TTCG	0.753	269	69	+	78	11	18795908
+Cdc45	CRISPRko	TCCAATTCATTAGTTCATACCTG	ATTC	0.6695	156	70	+	136	4	18810390
+Cdc45	CRISPRko	AAGCCAGCATCCTTGAGTCTGCT	TTCC	0.777	304	71	-	65	16	18786805
+Cdc45	CRISPRko	TTGACCTTGGACCTAGCCTCTGC	TTCC	0.6523	119	72	-	158	12	18795222
+Cdc45	CRISPRko	CCTGAAGCAAGTCAAGCAGAAAT	TTCC	0.795	330	73	-	53	13	18794834
+Cdc45	CRISPRko	TGTTGTTACAGGAAAGACATCCT	TTTG	0.8314	384	74	-	27	8	18801840
+Cdc45	CRISPRko	GTTTTATAACAGATCAAATTGCT	TTTT	0.7808	310	75	-	64	6	18807415
+Cdc45	CRISPRko	CTTACCCCATGTCTGCAAGGAAC	GTTA	0.709	225	76	+	107	12	18795111
+Cdc45	CRISPRko	CCGTCATAACCACCGAAACGAGG	TGTC	0.6127	22	77	-	209	11	18795879
+Cdc45	CRISPRko	GAGGTGCTTCTTGGCGAGCTCTA	CTTG	0.6613	160	78	+	143	15	18787038
+Cdc45	CRISPRko	ATTAGTTCATACCTGCTCTTTAT	ATTC	0.7001	218	79	+	114	4	18810398
+Cdc45	CRISPRko	CCAAGCCAGCATCCTTGAGTCTG	TTTT	0.7258	256	80	-	96	16	18786807
+Cdc45	CRISPRko	TTTGTCACTATTTCAGGAGATAG	TTTG	0.8423	406	81	-	22	7	18805433
+Cdc45	CRISPRko	GCCGCTGTGGCTATGTTCGTGAC	ATCC	0.6115	28	82	-	215	2	18811557
+Cdc45	CRISPRko	TGTGTGATACCCACAGGCCAGTG	TTTG	0.9222	440	83	-	11	5	18808764
+Cdc45	CRISPRko	TTGCAGTCGGCCATGGTGATGTT	TTCC	0.732	285	84	-	89	9	18798723
+Cdc45	CRISPRko	GTTTTCCTTGCAGTCGGCCATGG	TTTT	0.6594	173	85	-	146	9	18798730
+Cdc45	CRISPRko	CTTGAAGGGGAATCTGCGAGAAA	TTTC	0.9322	454	86	-	7	13	18794792
+Cdc45	CRISPRko	ATCAGGCTGAAGGATATCCAACA	CTTC	0.7692	342	87	+	68	5	18808825
+Cdc45	CRISPRko	ATTACTTCAGTAATTGAGCTGAA	TTTC	0.785	360	88	-	61	19	18781952
+Cdc45	CRISPRko	GACTGACAGACCAGTGGGTGCAT	GTTG	0.6371	130	89	-	176	10	18797365
+Cdc45	CRISPRko	CCAGGGCCCTTTTCTCTACTGCT	CTTC	0.7958	383	90	-	52	15	18786943
+Cdc45	CRISPRko	TGCCTACGACGACATCTTTAGAG	TTCC	0.7345	313	91	-	87	6	18807362
+Cdc45	CRISPRko	TCCTCATAAACTGTGGAGCCAAC	TTCA	0.7289	297	92	-	95	5	18808836
+Cdc45	CRISPRko	TGCTTGACTTGCTTCAGGGGAAG	TTTC	0.7678	350	93	+	70	13	18794858
+Cdc45	CRISPRko	TGTAGAGTCCGAGAGCTGGTGCT	ATTG	0.6296	122	94	+	185	18	18784847
+Cdc45	CRISPRko	GTGTGTGATACCCACAGGCCAGT	TTTT	0.796	393	95	-	51	5	18808765
+Cdc45	CRISPRko	TTGCGGAAATCGGTCACGAACAT	CTCC	0.5939	23	96	+	238	2	18811564
+Cdc45	CRISPRko	GCTGGTGTTGTACAGGCTTTCAT	TGTA	0.6588	208	97	+	147	12	18795190
+Cdc45	CRISPRko	TACTGCTCGTAGTCAAAGAGGAT	TTCA	0.6174	103	98	+	200	8	18801841
+Cdc45	CRISPRko	TTTTATAACAGATCAAATTGCTT	TTTG	0.7738	371	99	-	67	6	18807414
+Cdc45	CRISPRko	CAACGTTCACTGGCCTGTGGGTA	TTCA	0.6441	172	100	+	167	5	18808776
+Cdc45	CRISPRko	GTAATTGAGCTGAAGGCTGAGGA	TTCA	0.6125	89	101	-	210	19	18781943
+Cdc45	CRISPRko	TTGCCACCCAGAAACCGGAACCA	GTTC	0.653	195	102	+	157	4	18810446
+Cdc45	CRISPRko	TTGAAGGGGAATCTGCGAGAAAT	TTCC	0.8043	429	103	-	42	13	18794791
+Cdc45	CRISPRko	CTTGAACCCAAACTGAATGCTGA	TATG	0.6177	116	104	+	199	14	18793312
+Cdc45	CRISPRko	CATTACCTGGAGAGGCTATCGAG	TACC	0.602	59	105	+	231	14	18793193
+Cdc45	CRISPRko	CAAGGACCTGAATGACATGCTGT	TGTC	0.6932	281	106	-	120	9	18798679
+Cdc45	CRISPRko	TGGCCAGTGATGTCGTCTTTGCC	TTCC	0.7539	378	107	-	74	14	18793260
+Cdc45	CRISPRko	TGGGTGGCAAGAACTTGAAACTG	TTTC	0.9666	523	108	-	3	4	18810414
+Cdc45	CRISPRko	ATTAGAGACATGGTGGCAAAGAC	CTCC	0.6535	220	109	+	155	14	18793265
+Cdc45	CRISPRko	GGTGGGCCATTGTTGGACTGACA	TCCA	0.5899	46	110	-	242	10	18797380
+Cdc45	CRISPRko	TCACTATTTCAGGAGATAGTGGA	TTTG	0.8015	446	111	-	44	7	18805429
+Cdc45	CRISPRko	TTGGCGAGCTCTAGACCAAGGTA	CTTC	0.785	419	112	+	60	15	18787048
+Cdc45	CRISPRko	ACTATTTCAGGAGATAGTGGAGC	TGTC	0.6171	135	113	-	202	7	18805427
+Cdc45	CRISPRko	CCAGAGACTGATAGCTCGGATAG	CCCC	0.6079	109	114	-	219	17	18785081
+Cdc45	CRISPRko	TTCCAGAATGAAGTACGTGACTG	TTTC	0.7831	423	115	-	63	11	18795930
+Cdc45	CRISPRko	GTTCTCCTATTTCATCCTCATAA	TTCA	0.7337	377	116	-	88	5	18808850
+Cdc45	CRISPRko	TGGAGAGGCTATCGAGAGCCTGG	TACC	0.6632	277	117	+	142	14	18793200
+Cdc45	CRISPRko	GGTCGGGCTTTTGAGAAGGCAGC	TTTT	0.7407	401	118	-	84	18	18784855
+Cdc45	CRISPRko	AGAGAAGCGCACACGGTTAGAAG	CTTC	0.6552	267	119	-	152	6	18807284
+Cdc45	CRISPRko	TCCAGACTCACCTTCGGGCCTCC	CTTC	0.6243	191	120	+	191	7	18805392
+Cdc45	CRISPRko	ATACCTGCTCTTTATGTTCAAGA	GTTC	0.6868	324	121	+	125	4	18810406
+Cdc45	CRISPRko	TACCTGCTCTTTATGTTCAAGAT	TTCA	0.6489	250	122	+	162	4	18810407
+Cdc45	CRISPRko	CTTCAAGGAAACGTCCATGGACT	TCCC	0.596	108	123	+	235	13	18794827
+Cdc45	CRISPRko	CGTCATAACCACCGAAACGAGGC	GTCC	0.5597	4	124	-	287	11	18795878
+Cdc45	CRISPRko	TGCTGCCTTCTCAAAAGCCCGAC	TTTC	0.7972	480	125	+	50	18	18784873
+Cdc45	CRISPRko	GTTTGTCACTATTTCAGGAGATA	TTTT	0.6105	146	126	-	218	7	18805434
+Cdc45	CRISPRko	AACCCAAACTGAATGCTGAAAGT	CTTG	0.5906	112	127	+	240	14	18793316
+Cdc45	CRISPRko	CAGGGCACTCCAGATGTCACCCT	CTTC	0.5778	71	128	-	263	16	18786835
+Cdc45	CRISPRko	AGGTGGGCCATTGTTGGACTGAC	TTCC	0.7236	404	129	-	97	10	18797381
+Cdc45	CRISPRko	AGTTCTCCTATTTCATCCTCATA	TTTC	0.8971	569	130	-	15	5	18808851
+Cdc45	CRISPRko	GCTTGACTTGCTTCAGGGGAAGC	TTCT	0.6149	192	131	+	205	13	18794859
+Cdc45	CRISPRko	ATAATATTCATACTGCTCGTAGT	TCCC	0.6508	282	132	+	160	8	18801831
+Cdc45	CRISPRko	TTCCAGGTGGGCCATTGTTGGAC	TTCC	0.6036	149	133	-	228	10	18797385
+Cdc45	CRISPRko	AAAGCTCACCTGGAGGATCTTGC	ATTC	0.8407	561	134	+	23	3	18811317
+Cdc45	CRISPRko	TGTTTTCCTTGCAGTCGGCCATG	TTTT	0.632	244	135	-	183	9	18798731
+Cdc45	CRISPRko	CGCCGCTGTGGCTATGTTCGTGA	TATC	0.5496	2	136	-	304	2	18811558
+Cdc45	CRISPRko	TCAGTTCTCCTATTTCATCCTCA	TTTT	0.6966	381	137	-	117	5	18808853
+Cdc45	CRISPRko	CCCTCAGGACAGCAGTGACACAA	CTTA	0.7876	508	138	+	56	19	18781916
+Cdc45	CRISPRko	CTCCCTGGACAGGATGAAGGACA	CTTC	0.6728	353	139	-	134	14	18793331
+Cdc45	CRISPRko	GGAGATAGTGGAGCGAAACAGGA	TTCA	0.7235	425	140	-	98	7	18805418
+Cdc45	CRISPRko	TTTCTATCCGAGCTATCAGTCTC	CTTC	0.6439	286	141	+	168	17	18785097
+Cdc45	CRISPRko	ACGTTGGCTCCACAGTTTATGAG	GTCC	0.579	101	142	+	261	5	18808853
+Cdc45	CRISPRko	AAGTTCTTGCCACCCAGAAACCG	TTTC	0.8389	577	143	+	24	4	18810440
+Cdc45	CRISPRko	CCTGGAGAGGCTATCGAGAGCCT	ATTA	0.6077	189	144	+	220	14	18793198
+Cdc45	CRISPRko	AGTTCTTGCCACCCAGAAACCGG	TTCA	0.6799	369	145	+	130	4	18810441
+Cdc45	CRISPRko	TCCCTGGACAGGATGAAGGACAT	TTCC	0.6822	373	146	-	129	14	18793330
+Cdc45	CRISPRko	GCGAGCTCTAGACCAAGGTATAG	CTTG	0.6322	268	147	+	182	15	18787051
+Cdc45	CRISPRko	AAGCATAAATTCCTGGCCAGTGA	GTTC	0.7196	436	148	-	100	14	18793273
+Cdc45	CRISPRko	CAACAGGTCCACGTTGGCTCCAC	TATC	0.603	178	149	+	229	5	18808843
+Cdc45	CRISPRko	TGTGTGTGATACCCACAGGCCAG	TTTT	0.8375	587	150	-	25	5	18808766
+Cdc45	CRISPRko	AAGATATGCAGTTTCAAGTTCTT	GTTC	0.7295	450	151	+	94	4	18810425
+Cdc45	CRISPRko	TCCATGAAAGCCTGTACAACACC	TCCC	0.5851	141	152	-	249	12	18795174
+Cdc45	CRISPRko	CTGTTCCACGCTCAGCGGGGCAG	TGCC	0.5401	7	153	+	317	17	18785150
+Cdc45	CRISPRko	AAACTGCATATCTTGAACATAAA	CTTG	0.6548	336	154	-	153	4	18810397
+Cdc45	CRISPRko	GCTGAGCGTGGAACAGGGCACAG	CCCC	0.5762	113	155	-	268	17	18785124
+Cdc45	CRISPRko	AACAAGAGGATGACCTTGAGGTT	ATTA	0.6905	408	156	-	121	6	18807387
+Cdc45	CRISPRko	AGTAATTGAGCTGAAGGCTGAGG	CTTC	0.7093	439	157	-	106	19	18781944
+Cdc45	CRISPRko	GCCCATGCACAGACCAGAGCTTA	TTCT	0.5968	187	158	+	233	12	18795150
+Cdc45	CRISPRko	AGAATGAAGTACGTGACTGATGT	TTCC	0.7538	503	159	-	75	11	18795926
+Cdc45	CRISPRko	GGGCCTCCCACTCCCTGCGCTGC	CTTC	0.5713	107	160	+	273	7	18805407
+Cdc45	CRISPRko	ACAGCATGTCATTCAGGTCCTTG	TACC	0.6218	266	161	+	194	9	18798698
+Cdc45	CRISPRko	GTCGGCCATGGTGATGTTTGACC	TGCA	0.5395	19	162	-	318	9	18798718
+Cdc45	CRISPRko	CTGCCTACGACGACATCTTTAGA	GTTC	0.7189	453	163	-	101	6	18807363
+Cdc45	CRISPRko	GTCATAACCACCGAAACGAGGCG	TCCC	0.5447	38	164	-	311	11	18795877
+Cdc45	CRISPRko	CAGTTCTCCTATTTCATCCTCAT	TTTT	0.6493	338	165	-	161	5	18808852
+Cdc45	CRISPRko	GTTTGGGTTCAAGCATAAATTCC	TTCA	0.6581	363	166	-	149	14	18793283
+Cdc45	CRISPRko	CCTCCGCCTCGTTTCGGTGGTTA	TTCT	0.5906	180	167	+	241	11	18795892
+Cdc45	CRISPRko	AGGAGATAGTGGAGCGAAACAGG	TTTC	0.9435	652	168	-	5	7	18805419
+Cdc45	CRISPRko	AGAAGGCAGCAGAAAGCACCAGC	TTTG	0.8703	626	169	-	19	18	18784842
+Cdc45	CRISPRko	GGTTTCTGGGTGGCAAGAACTTG	TTCC	0.7974	566	170	-	49	4	18810420
+Cdc45	CRISPRko	TCAGGACTCTCCATTAGAGACAT	CTTC	0.6059	215	171	+	225	14	18793253
+Cdc45	CRISPRko	CCTTTCATTACTTCAGTAATTGA	TTTT	0.7504	509	172	-	80	19	18781958
+Cdc45	CRISPRko	TGGAGGGACCAGTGCTGATACAG	TTCA	0.7181	465	173	+	102	12	18795212
+Cdc45	CRISPRko	GACAGTGACGGTGATGGGTCAGA	TTCA	0.6891	421	174	-	124	6	18807313
+Cdc45	CRISPRko	AGCTCTGGTCTGTGCATGGGCAG	TTTA	0.8283	614	175	-	29	12	18795129
+Cdc45	CRISPRko	CCTTGCAGTCGGCCATGGTGATG	TTTT	0.6323	316	176	-	181	9	18798725
+Cdc45	CRISPRko	ATCCAAGTGCTCACCGAGTACAC	CTCC	0.606	233	177	+	224	16	18786776
+Cdc45	CRISPRko	TGTGCGCTACATACCACAGCATG	TGTA	0.6826	427	178	+	127	9	18798683
+Cdc45	CRISPRko	CGAGGCCACAAAGAGAAGGACCC	CGTC	0.5695	133	179	+	277	3	18811362
+Cdc45	CRISPRko	TGCCCATGCACAGACCAGAGCTT	TTTC	0.8998	661	180	+	14	12	18795149
+Cdc45	CRISPRko	AAGCTCACCTGGAGGATCTTGCA	TTCA	0.6392	343	181	+	174	3	18811318
+Cdc45	CRISPRko	TTACTTCAGTAATTGAGCTGAAG	TTCA	0.6335	334	182	-	179	19	18781951
+Cdc45	CRISPRko	AGACAGTGACGGTGATGGGTCAG	TTTC	0.8241	623	183	-	35	6	18807314
+Cdc45	CRISPRko	TCCTATTTCATCCTCATAAACTG	GTTC	0.6511	375	184	-	159	5	18808846
+Cdc45	CRISPRko	TGTTCAAGATATGCAGTTTCAAG	TTTA	0.8069	615	185	+	40	4	18810420
+Cdc45	CRISPRko	TCTCTAAAGATGTCGTCGTAGGC	TTCA	0.6548	388	186	+	154	6	18807380
+Cdc45	CRISPRko	AGACTCACCTTCGGGCCTCCCAC	CTCC	0.602	241	187	+	230	7	18805395
+Cdc45	CRISPRko	CTTCCAGGTGGGCCATTGTTGGA	TTTC	0.7007	478	188	-	113	10	18797386
+Cdc45	CRISPRko	ATGTGTCCCGTCATAACCACCGA	CGTC	0.5406	74	189	-	316	11	18795886
+Cdc45	CRISPRko	CAGGAAAGACATCCTCTTTGACT	GTTA	0.7987	612	190	-	48	8	18801832
+Cdc45	CRISPRko	AGCATTCAGTTTGGGTTCAAGCA	TTTC	0.7939	599	191	-	55	14	18793291
+Cdc45	CRISPRko	ATCCTCTGCTTCATCTCTAAAGA	CTTC	0.6569	412	192	+	150	6	18807367
+Cdc45	CRISPRko	AATCATTTCTCGCAGATTCCCCT	CTTC	0.6109	279	193	+	217	13	18794806
+Cdc45	CRISPRko	CTTCTACAGAGGGTCCTTCTCTT	TTTG	0.7311	533	194	-	90	3	18811353
+Cdc45	CRISPRko	GAGATGAAGCAGAGGATGAAGAT	TTTA	0.827	654	195	-	30	6	18807342
+Cdc45	CRISPRko	GACCGTCTCGTAGAACTCCTTGC	TGTG	0.5098	9	196	+	353	2	18811545
+Cdc45	CRISPRko	TCCAGAATGAAGTACGTGACTGA	TTCT	0.6062	273	197	-	223	11	18795929
+Cdc45	CRISPRko	TGTCTCCATAGACAAAGAATCGA	CTCC	0.616	312	198	-	204	17	18785182
+Cdc45	CRISPRko	CCACCCAGAAACCGGAACCAGCG	CTTG	0.5762	188	199	+	267	4	18810449
+Cdc45	CRISPRko	AGCATAAATTCCTGGCCAGTGAT	TTCA	0.6935	494	200	-	119	14	18793272
+Cdc45	CRISPRko	ATCCGAGCTATCAGTCTCTGGGG	TTCT	0.5313	82	201	+	329	17	18785102
+Cdc45	CRISPRko	CAGATTCTTCAATCATTTCTCGC	TTTG	0.7498	578	202	+	81	13	18794796
+Cdc45	CRISPRko	CACATTCACAACGTTCACTGGCC	TGTA	0.5324	86	203	+	327	5	18808768
+Cdc45	CRISPRko	ATACTGCTCGTAGTCAAAGAGGA	ATTC	0.8052	659	204	+	41	8	18801840
+Cdc45	CRISPRko	AACGTGGACCTGTTGGATATCCT	AGCC	0.5055	18	205	-	362	5	18808816
+Cdc45	CRISPRko	CCCTTCCAGGGCACTCCAGATGT	CTTC	0.6681	468	206	-	138	16	18786841
+Cdc45	CRISPRko	GAGCTATCAGTCTCTGGGGGGAT	ATCC	0.5961	278	207	+	234	17	18785106
+Cdc45	CRISPRko	TCAGATCTGTTCATACTTACCGA	TGTG	0.5873	254	208	+	246	8	18801802
+Cdc45	CRISPRko	CAAGCACACAAGGCATCCACGTC	CTTG	0.723	549	209	+	99	3	18811339
+Cdc45	CRISPRko	GCCAACCATGCTTTCTTCCAGAA	TTCA	0.6534	438	210	-	156	11	18795945
+Cdc45	CRISPRko	TCCGCCTCGTTTCGGTGGTTATG	CTCC	0.5562	168	211	+	293	11	18795894
+Cdc45	CRISPRko	CTCCGGTCCTCAGCCTTCAGCTC	CTTG	0.5643	194	212	+	282	19	18781956
+Cdc45	CRISPRko	GTATACGCTGGTTCCGGTTTCTG	TCCA	0.4974	1	213	-	379	4	18810435
+Cdc45	CRISPRko	TTTCATTACTTCAGTAATTGAGC	TTCC	0.7621	622	214	-	71	19	18781956
+Cdc45	CRISPRko	CCTGGTGATGGCTGCCCCGCTGA	TGCC	0.5113	60	215	-	352	17	18785142
+Cdc45	CRISPRko	AGTGCTCACCGAGTACACAAAGG	TCCA	0.5358	121	216	+	322	16	18786781
+Cdc45	CRISPRko	AGGTATCCGCCGCTGTGGCTATG	TTCT	0.6397	422	217	-	173	2	18811565
+Cdc45	CRISPRko	GATATCCTTCAGCCTGATGAAGA	GTTG	0.5758	231	218	-	269	5	18808801
+Cdc45	CRISPRko	AGTATACGCTGGTTCCGGTTTCT	GTCC	0.5007	27	219	-	371	4	18810436
+Cdc45	CRISPRko	TTGGACATCATCCACGCCAGGTC	GTCC	0.583	264	220	+	254	9	18798718
+Cdc45	CRISPRko	GCATCCTGCAGCGTCATGTGTCC	GTTG	0.5486	167	221	-	305	11	18795901
+Cdc45	CRISPRko	CCTCTTCTAACCGTGTGCGCTTC	CTCA	0.5068	62	222	+	358	6	18807300
+Cdc45	CRISPRko	TGGTGATGGCTGCCCCGCTGAGC	CCCC	0.5304	115	223	-	332	17	18785140
+Cdc45	CRISPRko	GGGACAGACCACTTCATCCAGGC	CTCA	0.5041	45	224	-	367	14	18793198
+Cdc45	CRISPRko	ACCACGTCCAGTATACGCTGGTT	TGTG	0.4943	11	225	-	385	4	18810445
+Cdc45	CRISPRko	CCATAATATTCATACTGCTCGTA	TGTC	0.6119	361	226	+	213	8	18801829
+Cdc45	CRISPRko	TCCTCCGCCTCGTTTCGGTGGTT	GTTC	0.5745	247	227	+	271	11	18795891
+Cdc45	CRISPRko	CTAAGGTATTCAAAGCTCACCTG	CTTC	0.5843	287	228	+	252	3	18811306
+Cdc45	CRISPRko	TGGACAAGCTATACCTTGGTCTA	AACC	0.498	41	229	-	376	15	18787040
+Cdc45	CRISPRko	CTTCCAGGGCACTCCAGATGTCA	TCCC	0.5733	252	230	-	272	16	18786839
+Cdc45	CRISPRko	AGCGTATACTGGACGTGGTCACA	AACC	0.5445	177	231	+	312	4	18810468
+Cdc45	CRISPRko	GCTGCCTTCTCAAAAGCCCGACC	TTCT	0.5531	210	232	+	297	18	18784874
+Cdc45	CRISPRko	GGTCCTCAGCCTTCAGCTCAATT	CTCC	0.5713	258	233	+	274	19	18781960
+Cdc45	CRISPRko	GAGTCCGAGAGCTGGTGCTTTCT	TGTA	0.562	238	234	+	284	18	18784851
+Cdc45	CRISPRko	TACAGGCTTTCATGGAGGGACCA	GTTG	0.5946	333	235	+	237	12	18795200
+Cdc45	CRISPRko	TGGCGAGCTCTAGACCAAGGTAT	TTCT	0.5012	68	236	+	370	15	18787049
+Cdc45	CRISPRko	GGATCGTGGAATGGGAGTCACCT	ATCA	0.5387	170	237	+	319	2	18811515
+Cdc45	CRISPRko	AGGAAACGTCCATGGACTGAAAT	TTCA	0.6278	433	238	+	188	13	18794832
+Cdc45	CRISPRko	TGAGTGTCATTGTACACATTCAC	TACC	0.6904	567	239	+	122	5	18808754
+Cdc45	CRISPRko	GAGACAGCTGGCGATGGTCTGTT	TACA	0.505	81	240	+	366	15	18787005
+Cdc45	CRISPRko	TGGACCGTCTCGTAGAACTCCTT	GTTG	0.585	319	241	+	251	2	18811543
+Cdc45	CRISPRko	CTGAAGCAAGTCAAGCAGAAATT	TCCC	0.6068	379	242	-	222	13	18794833
+Cdc45	CRISPRko	TCAGGCTGAAGGATATCCAACAG	TTCA	0.6134	407	243	+	208	5	18808826
+Cdc45	CRISPRko	GTCCATGGACGTTTCCTTGAAGG	TTCA	0.6122	403	244	-	211	13	18794807
+Cdc45	CRISPRko	CCCTTCAAGGAAACGTCCATGGA	ATTC	0.6636	545	245	+	140	13	18794825
+Cdc45	CRISPRko	TCTGTACCAACCTCGTCACTTCC	TGTC	0.5331	179	246	-	324	15	18786965
+Cdc45	CRISPRko	TCATGCACCCACTGGTCTGTCAG	CTTG	0.6184	431	247	+	198	10	18797382
+Cdc45	CRISPRko	TTAAACAAGAGGATGACCTTGAG	CTTA	0.6597	538	248	-	145	6	18807390
+Cdc45	CRISPRko	GCTTGCTCCCTCCAGGCTTCCCC	TACA	0.5024	93	249	-	368	13	18794855
+Cdc45	CRISPRko	CTGAGTGATCTTGTCATGCACCC	CTTA	0.708	613	250	+	110	10	18797369
+Cdc45	CRISPRko	CCTTCCAGGGCACTCCAGATGTC	TTCC	0.6974	602	251	-	116	16	18786840
+Cdc45	CRISPRko	AACAGGTCCACGTTGGCTCCACA	ATCC	0.492	54	252	+	391	5	18808844
+Cdc45	CRISPRko	TTGTGTGTGATACCCACAGGCCA	TTTT	0.7087	625	253	-	108	5	18808767
+Cdc45	CRISPRko	TCCCGTCATAACCACCGAAACGA	TGTG	0.522	162	254	-	340	11	18795881
+Cdc45	CRISPRko	AGGGCCCTTTTCTCTACTGCTCA	TCCC	0.6607	556	255	-	144	15	18786941
+Cdc45	CRISPRko	ACAGGCCAGTGAACGTTGTGAAT	ACCC	0.4999	100	256	-	372	5	18808752
+Cdc45	CRISPRko	GCGGGGCAGCCATCACCAGGGGC	CTCA	0.547	230	257	+	308	17	18785164
+Cdc45	CRISPRko	AGATCTGTTCATACTTACCGATG	TGTC	0.5791	331	258	+	260	8	18801804
+Cdc45	CRISPRko	AGGCCCTGTTTCAGTGTGACCAC	CTCC	0.6036	397	259	-	227	4	18810463
+Cdc45	CRISPRko	TTAGTTCATACCTGCTCTTTATG	TTCA	0.6754	595	260	+	132	4	18810399
+Cdc45	CRISPRko	TGAGCCATCCTTCTCAGGACTCT	TCCC	0.646	532	261	+	164	14	18793240
+Cdc45	CRISPRko	ACAGTTTATGAGGATGAAATAGG	CTCC	0.6568	558	262	+	151	5	18808864
+Cdc45	CRISPRko	TGTGGCTTGGAGGTGCTTCTTGG	GTTG	0.5691	306	263	+	278	15	18787029
+Cdc45	CRISPRko	CCTATTTCATCCTCATAAACTGT	TTCT	0.6144	452	264	-	206	5	18808845
+Cdc45	CRISPRko	TGCAAGGAACTCCTGGAGTCGTT	TGTC	0.5361	223	265	+	321	12	18795124
+Cdc45	CRISPRko	CCTGGTTGTGGACCGTCTCGTAG	GTCA	0.4924	88	266	+	389	2	18811535
+Cdc45	CRISPRko	CGGGTAAGGAAGTCTAACCTCCA	TGCC	0.5131	171	267	+	348	15	18786933
+Cdc45	CRISPRko	GACGATGCAAGCTGCTGCCCCTG	AATC	0.4774	36	268	-	416	17	18785161
+Cdc45	CRISPRko	GGGCACTCCAGATGTCACCCTGT	TCCA	0.4753	35	269	-	418	16	18786833
+Cdc45	CRISPRko	GTTCATACCTGCTCTTTATGTTC	ATTA	0.5054	144	270	+	364	4	18810402
+Cdc45	CRISPRko	TGTGCCTTTAGCTTTTTTGGTCG	GTTC	0.639	525	271	-	175	18	18784873
+Cdc45	CRISPRko	CCGAGTACACAAAGGACTTGAGC	CTCA	0.4932	99	272	+	388	16	18786789
+Cdc45	CRISPRko	CATGGACTGAAATTTCTGCTTGA	CGTC	0.5599	307	273	+	286	13	18794842
+Cdc45	CRISPRko	GACCTAGCCTCTGCCTGGTACTG	CTTG	0.4909	95	274	-	392	12	18795213
+Cdc45	CRISPRko	GGGTGGCAAGAACTTGAAACTGC	TTCT	0.6469	553	275	-	163	4	18810413
+Cdc45	CRISPRko	AGGTTCCTGCCTACGACGACATC	CTTG	0.4815	64	276	-	408	6	18807369
+Cdc45	CRISPRko	CCTGGAGGATCTTGCAAGCACAC	CTCA	0.5823	370	277	+	255	3	18811325
+Cdc45	CRISPRko	GGAACCAGCGTATACTGGACGTG	AACC	0.5124	183	278	+	350	4	18810462
+Cdc45	CRISPRko	ACTGGCCTGTGGGTATCACACAC	GTTC	0.6419	544	279	+	170	5	18808784
+Cdc45	CRISPRko	CCAATTCATTAGTTCATACCTGC	TTCT	0.5896	396	280	+	244	4	18810391
+Cdc45	CRISPRko	ACACAACAGACCATCGCCAGCTG	AGCC	0.4808	69	281	-	410	15	18786990
+Cdc45	CRISPRko	AGGTCAAACATCACCATGGCCGA	CGCC	0.5545	300	282	+	295	9	18798736
+Cdc45	CRISPRko	CTTATTAAACAAGAGGATGACCT	ATTG	0.5917	414	283	-	239	6	18807394
+Cdc45	CRISPRko	GCCTGATGAAGACAGTATATTTT	TTCA	0.6284	520	284	-	186	5	18808790
+Cdc45	CRISPRko	CAGGCTTTCATGGAGGGACCAGT	TGTA	0.5542	302	285	+	296	12	18795202
+Cdc45	CRISPRko	TCAATCATTTCTCGCAGATTCCC	TTCT	0.531	240	286	+	330	13	18794804
+Cdc45	CRISPRko	CGTTGGCTCCACAGTTTATGAGG	TCCA	0.4675	33	287	+	434	5	18808854
+Cdc45	CRISPRko	TACAGCTTGCTCCCTCCAGGCTT	TTCA	0.5325	253	288	-	326	13	18794859
+Cdc45	CRISPRko	GCCTCGGACGTGGATGCCTTGTG	TGTG	0.5019	169	289	-	369	3	18811326
+Cdc45	CRISPRko	CGGTTTCTGGGTGGCAAGAACTT	GTTC	0.6433	571	290	-	169	4	18810421
+Cdc45	CRISPRko	TGAGAAGGATGGCTCAGGGACAG	GTCC	0.632	542	291	-	184	14	18793214
+Cdc45	CRISPRko	TTTTGTTTGTCACTATTTCAGGA	TTTC	0.6705	640	292	-	135	7	18805438
+Cdc45	CRISPRko	ATGCACCCACTGGTCTGTCAGTC	TGTC	0.6334	551	293	+	180	10	18797384
+Cdc45	CRISPRko	ACTTCTCCAGACTCACCTTCGGG	ATCC	0.5992	447	294	+	232	7	18805387
+Cdc45	CRISPRko	ATGGACGTTTCCTTGAAGGGGAA	GTCC	0.6052	461	295	-	226	13	18794803
+Cdc45	CRISPRko	AGATATGCAGTTTCAAGTTCTTG	TTCA	0.6449	583	296	+	165	4	18810426
+Cdc45	CRISPRko	TGTACAACACCAGCTACACAGCA	AGCC	0.5547	329	297	-	294	12	18795162
+Cdc45	CRISPRko	GTAGGCAGGAACCTCAAGGTCAT	CGTC	0.5767	387	298	+	265	6	18807397
+Cdc45	CRISPRko	CGCTACATACCACAGCATGTCAT	TGTG	0.4986	174	299	+	373	9	18798687
+Cdc45	CRISPRko	TGCCACCCAGAAACCGGAACCAG	TTCT	0.6144	506	300	+	207	4	18810447
+Cdc45	CRISPRko	ATTGTGTAGAGTCCGAGAGCTGG	AGTA	0.5341	280	301	+	323	18	18784843
+Cdc45	CRISPRko	CCATGTCTGCAAGGAACTCCTGG	TACC	0.6364	574	302	+	177	12	18795117
+Cdc45	CRISPRko	CATAATATTCATACTGCTCGTAG	GTCC	0.5566	346	303	+	291	8	18801830
+Cdc45	CRISPRko	GTCTAGAGCTCGCCAAGAAGCAC	CTTG	0.4704	78	304	-	426	15	18787022
+Cdc45	CRISPRko	ACCTGGTTGTGGACCGTCTCGTA	AGTC	0.4557	15	305	+	459	2	18811534
+Cdc45	CRISPRko	ACAACGTTCACTGGCCTGTGGGT	ATTC	0.4542	10	306	+	463	5	18808775
+Cdc45	CRISPRko	TTACAGGAAAGACATCCTCTTTG	GTTG	0.5597	362	307	-	288	8	18801835
+Cdc45	CRISPRko	ATACAGCTTGCTCCCTCCAGGCT	ATTC	0.4899	154	308	-	394	13	18794860
+Cdc45	CRISPRko	TCCAGGCTCTCGATAGCCTCTCC	TTCA	0.6119	515	309	-	214	14	18793182
+Cdc45	CRISPRko	GAAAACAGGGTGACATCTGGAGT	CTTG	0.607	505	310	+	221	16	18786848
+Cdc45	CRISPRko	AAGGAAACGTCCATGGACTGAAA	CTTC	0.6235	564	311	+	192	13	18794831
+Cdc45	CRISPRko	TAACCTCCATGAGTGAGCAGTAG	AGTC	0.4633	61	312	+	444	15	18786947
+Cdc45	CRISPRko	AATGTGTACAATGACACTCAGGT	TGTG	0.611	517	313	-	216	5	18808732
+Cdc45	CRISPRko	GTACAGAGACAGCTGGCGATGGT	GTTG	0.636	594	314	+	178	15	18787000
+Cdc45	CRISPRko	GGACTCTCCATTAGAGACATGGT	CTCA	0.4573	42	315	+	455	14	18793256
+Cdc45	CRISPRko	CCGCTGAGCGTGGAACAGGGCAC	TGCC	0.4593	52	316	-	451	17	18785126
+Cdc45	CRISPRko	TACTCGAAGGAGATCCGGGTGCA	CTCA	0.4509	14	317	+	471	11	18795849
+Cdc45	CRISPRko	TTGTGTGCTTGCAAGATCCTCCA	TGCC	0.506	236	318	-	360	3	18811308
+Cdc45	CRISPRko	TTGGTCGGGCTTTTGAGAAGGCA	TTTT	0.4971	200	319	-	380	18	18784857

resources/KO_SA_human.txt

@@ -0,0 +1,1001 @@
+Target Gene Symbol	CRISPR Mechanism	sgRNA Sequence	PAM Sequence	On-Target Efficacy Score	Off-Target Rank	Combined Rank	Strand of sgRNA	On-Target Rank	Exon Number	sgRNA Cut Position (1-based)
+TSPAN6	CRISPRko	GACCAGTTCGACCAAAAAAAC	GAGAG	0.9043	10	1	+	1	3	100635229
+TSPAN6	CRISPRko	TTGTTGTGGTGTCACCGATTA	TAGAG	0.6794	2	2	-	14	5	100633517
+TSPAN6	CRISPRko	AAAACAACCAAAGGTGCCCAA	AAGAA	0.835	13	3	+	6	2	100635612
+TSPAN6	CRISPRko	TACCAGTAGCAATGAGCACGA	AGGGG	0.6544	6	4	+	20	2	100635647
+TSPAN6	CRISPRko	GTTTAGTCTGCAGTCTCCGAG	ACGGG	0.5838	8	5	+	25	1	100636679
+TSPAN6	CRISPRko	TTTAGTCTGCAGTCTCCGAGA	CGGGG	0.572	5	6	+	28	1	100636680
+TSPAN6	CRISPRko	ACTTGAAGATTGTACTCCACA	GAGAG	0.8482	36	7	-	4	5	100633436
+TSPAN6	CRISPRko	ACCAGTAGCAATGAGCACGAA	GGGGA	0.5364	9	8	+	33	2	100635648
+TSPAN6	CRISPRko	GTCAGAAATGGGAGTCGTTGC	AGGAA	0.7659	35	9	-	9	6	100632519
+TSPAN6	CRISPRko	TTATAAAGGTGATGACCATTA	TAGAG	0.8751	41	10	-	3	6	100632544
+TSPAN6	CRISPRko	GTGTAGATTAGCAGAACGCTC	TTGAA	0.5069	1	11	+	43	1	100636639
+TSPAN6	CRISPRko	AGTCGTTGCAGGAATTTCCTT	TGGAG	0.768	39	12	-	8	6	100632507
+TSPAN6	CRISPRko	GCGACCAGTTCGACCAAAAAA	ACGAG	0.5282	14	13	+	35	3	100635227
+TSPAN6	CRISPRko	TTCGACCAAAAAAACGAGAGT	CAGAA	0.5594	20	14	+	29	3	100635235
+TSPAN6	CRISPRko	GTGGTTACCACTTACCGTATT	TTGGA	0.4943	4	15	+	48	4	100633934
+TSPAN6	CRISPRko	CATGGCTTCTATAATCTCCTG	TAGAG	0.7624	42	16	+	10	4	100633972
+TSPAN6	CRISPRko	ACGGTACCAGTAGCAATGAGC	ACGAA	0.4974	7	17	+	46	2	100635643
+TSPAN6	CRISPRko	AGTCAGAAATGGGAGTCGTTG	CAGGA	0.5099	15	18	-	42	6	100632520
+TSPAN6	CRISPRko	CTAGTCATGGCGTCCCCGTCT	CGGAG	0.469	3	19	-	54	1	100636683
+TSPAN6	CRISPRko	TTTGAAGCAGTATAACTCTAC	AGGAG	0.577	34	20	-	27	4	100633977
+TSPAN6	CRISPRko	TACTCAGAAAAAGGATTTCCT	AAGAG	0.8353	57	21	-	5	5	100633471
+TSPAN6	CRISPRko	GCCCCAAATGCCAACTGCAAG	AAGGA	0.7014	49	22	+	13	2	100635726
+TSPAN6	CRISPRko	GCGAGAAAGATTCCAATCAGC	TAGAA	0.5198	23	23	+	40	7	100630865
+TSPAN6	CRISPRko	AAACTTGAAGATTGTACTCCA	CAGAG	0.4757	16	24	-	50	5	100633438
+TSPAN6	CRISPRko	AACTCTTAGGAAATCCTTTTT	CTGAG	0.6779	54	25	+	15	5	100633479
+TSPAN6	CRISPRko	ATGGCACGAGAGAGGCAGTAG	GCGAG	0.6134	48	26	+	22	7	100630844
+TSPAN6	CRISPRko	GGTGACACCACAACAATGCAA	CTGAA	0.5009	25	27	+	45	5	100633537
+TSPAN6	CRISPRko	TGGTTATTTGTTATGGCACGA	GAGAG	0.5206	32	28	+	39	7	100630832
+TSPAN6	CRISPRko	ACTTGGAAGCAAGCAACTCCA	AAGGA	0.4706	19	29	+	53	6	100632501
+TSPAN6	CRISPRko	AGGTGATGACCATTATAGAGT	CAGAA	0.4652	17	30	-	56	6	100632538
+TSPAN6	CRISPRko	GACCATTATAGAGTCAGAAAT	GGGAG	0.8988	72	31	-	2	6	100632531
+TSPAN6	CRISPRko	GGCACGAGAGAGGCAGTAGGC	GAGAA	0.476	26	32	+	49	7	100630846
+TSPAN6	CRISPRko	CTTGGAAGCAAGCAACTCCAA	AGGAA	0.5209	38	33	+	38	6	100632502
+TSPAN6	CRISPRko	ATGACCATTATAGAGTCAGAA	ATGGG	0.4737	24	34	-	52	6	100632533
+TSPAN6	CRISPRko	GAGATGCAGACAAAGTAAACA	ATGAA	0.66	58	35	-	18	5	100633413
+TSPAN6	CRISPRko	CTATAGATTAAGAACAGCTTT	AAGAA	0.6542	55	36	-	21	4	100634018
+TSPAN6	CRISPRko	TTAGTCTGCAGTCTCCGAGAC	GGGGA	0.4104	11	37	+	66	1	100636681
+TSPAN6	CRISPRko	GTGCCATAACAAATAACCAGT	ATGAG	0.6596	59	38	-	19	7	100630815
+TSPAN6	CRISPRko	GTATAACTCTACAGGAGATTA	TAGAA	0.5288	45	39	-	34	4	100633968
+TSPAN6	CRISPRko	ACTGGTTATTTGTTATGGCAC	GAGAG	0.4507	21	40	+	60	7	100630830
+TSPAN6	CRISPRko	CTTTGAAGCAGTATAACTCTA	CAGGA	0.3874	12	41	-	72	4	100633978
+TSPAN6	CRISPRko	AGTCAGAAACATTGCATACTG	CAGGA	0.6741	68	42	+	16	3	100635253
+TSPAN6	CRISPRko	TCTGCATGGATGCTAAAACTG	GTGAG	0.8279	78	43	-	7	2	100635561
+TSPAN6	CRISPRko	GCAGTTGGCATTTGGGGCAAG	GTGAG	0.5232	51	44	-	36	2	100635708
+TSPAN6	CRISPRko	TCGAACTGGTCGCTGCCATCG	TAGGA	0.5955	65	45	-	24	3	100635204
+TSPAN6	CRISPRko	GACTGGTTTAGTCTGCAGTCT	CCGAG	0.5784	63	46	+	26	1	100636674
+TSPAN6	CRISPRko	AGAACAGCTTTAAGAATAATT	ATGAG	0.4515	31	47	-	59	4	100634008
+TSPAN6	CRISPRko	TACTTTGTCTGCATCTCTCTG	TGGAG	0.7259	79	48	+	12	5	100633432
+TSPAN6	CRISPRko	TAATTCAGGCTTACCTCATGT	CTGAA	0.3764	18	49	+	74	3	100635182
+TSPAN6	CRISPRko	GAAAATAAAAGTGTAGATTAG	CAGAA	0.4215	29	50	+	63	1	100636629
+TSPAN6	CRISPRko	ATTGGTGGCCTTCTCATTTAA	AAGAG	0.3794	22	51	+	73	2	100635675
+TSPAN6	CRISPRko	ATACTGGTTATTTGTTATGGC	ACGAG	0.401	28	52	+	70	7	100630828
+TSPAN6	CRISPRko	GCATTTGGGGCAAGGTGAGCC	TGGAG	0.4739	47	53	-	51	2	100635701
+TSPAN6	CRISPRko	CTTTGGTTGTTTTGCTACCTG	CCGAG	0.5229	62	54	-	37	2	100635589
+TSPAN6	CRISPRko	ATAATGACGGTACCAGTAGCA	ATGAG	0.6008	76	55	+	23	2	100635637
+TSPAN6	CRISPRko	TCGTAGGATTTGTTTTCAGAC	ATGAG	0.5024	56	56	-	44	3	100635186
+TSPAN6	CRISPRko	ATTTGGGGCAAGGTGAGCCTG	GAGAA	0.737	89	57	-	11	2	100635699
+TSPAN6	CRISPRko	TGCTACCTGCCGAGCTTCTGC	ATGGA	0.6671	87	58	-	17	2	100635577
+TSPAN6	CRISPRko	GTACCAGTAGCAATGAGCACG	AAGGG	0.5398	73	59	+	31	2	100635646
+TSPAN6	CRISPRko	TGGTGTCACCGATTATAGAGA	TTGGA	0.4057	37	60	-	69	5	100633511
+TSPAN6	CRISPRko	CACCTTTATAAAACAACCCTA	ATGGG	0.4661	52	61	+	55	6	100632569
+TSPAN6	CRISPRko	TCACCAGTTTTAGCATCCATG	CAGAA	0.4956	61	62	+	47	2	100635572
+TSPAN6	CRISPRko	GCTAGTCATGGCGTCCCCGTC	TCGGA	0.3692	33	63	-	76	1	100636684
+TSPAN6	CRISPRko	TGTGTTTCTTTGCTATAGATT	AAGAA	0.3304	27	64	-	83	4	100634030
+TSPAN6	CRISPRko	CCTATTTTATTTCTAGCTGAT	TGGAA	0.5169	71	65	-	41	7	100630865
+TSPAN6	CRISPRko	TTCTGACTCTCGTTTTTTTGG	TCGAA	0.438	53	66	-	61	3	100635225
+TSPAN6	CRISPRko	GCTAATCTACACTTTTATTTT	CTGGG	0.5377	82	67	-	32	1	100636615
+TSPAN6	CRISPRko	TGACCATTATAGAGTCAGAAA	TGGGA	0.4094	50	68	-	67	6	100632532
+TSPAN6	CRISPRko	AACAGCTTTAAGAATAATTAT	GAGAA	0.3006	30	69	-	87	4	100634006
+TSPAN6	CRISPRko	ATACTAATTATTACTCAGAAA	AAGGA	0.5429	88	70	-	30	5	100633482
+TSPAN6	CRISPRko	GGACAGATACTAATTATTACT	CAGAA	0.3595	44	71	-	77	5	100633488
+TSPAN6	CRISPRko	TTCCTAAGAGTTGCTGTAAAC	TTGAA	0.3303	40	72	-	84	5	100633455
+TSPAN6	CRISPRko	AAACCAGTCATTACTTGTTTC	AAGAG	0.3368	43	73	-	82	1	100636647
+TSPAN6	CRISPRko	CTTGCCCCAAATGCCAACTGC	AAGAA	0.4265	66	74	+	62	2	100635723
+TSPAN6	CRISPRko	GAGTCGTTGCAGGAATTTCCT	TTGGA	0.4157	67	75	-	64	6	100632508
+TSPAN6	CRISPRko	CTTCAAGTTTACAGCAACTCT	TAGGA	0.3103	46	76	+	85	5	100633464
+TSPAN6	CRISPRko	TTACTTTGTCTGCATCTCTCT	GTGGA	0.4569	74	77	+	58	5	100633431
+TSPAN6	CRISPRko	TATCCTTCTTGCAGTTGGCAT	TTGGG	0.4116	69	78	-	65	2	100635718
+TSPAN6	CRISPRko	ATCTACACTTTTATTTTCTGG	GTGAG	0.4614	83	79	-	57	1	100636611
+TSPAN6	CRISPRko	TTCAAGTTTACAGCAACTCTT	AGGAA	0.3544	64	80	+	78	5	100633465
+TSPAN6	CRISPRko	ACTGGTACCGTCATTATTCTT	TTGGG	0.3079	60	81	-	86	2	100635618
+TSPAN6	CRISPRko	ATCCTTCTTGCAGTTGGCATT	TGGGG	0.3907	77	82	-	71	2	100635717
+TSPAN6	CRISPRko	TGGTGGCCTTCTCATTTAAAA	GAGAA	0.3725	75	83	+	75	2	100635677
+TSPAN6	CRISPRko	GGCATTTGGGGCAAGGTGAGC	CTGGA	0.3399	70	84	-	80	2	100635702
+TSPAN6	CRISPRko	CTACACTTTTATTTTCTGGGT	GAGAG	0.4073	85	85	-	68	1	100636609
+TSPAN6	CRISPRko	AAGAATAATTATGAGAAGGCT	TTGAA	0.3474	80	86	-	79	4	100633997
+TSPAN6	CRISPRko	AGAATTACTTTTCTCTTTTAA	ATGAG	0.337	86	87	-	81	2	100635677
+TSPAN6	CRISPRko	TCCTATTTTATTTCTAGCTGA	TTGGA	0.2904	81	88	-	88	7	100630866
+TSPAN6	CRISPRko	AATTACTTTTCTCTTTTAAAT	GAGAA	0.2599	84	89	-	89	2	100635675
+TNMD	CRISPRko	GCTCCCCCAAAACAGGACAAT	TAGAG	0.808	9	1	-	4	2	100585315
+TNMD	CRISPRko	TAAGAATGTCATACCTGATAT	TAGAG	0.7168	12	2	-	11	5	100597654
+TNMD	CRISPRko	CTCACTTGCTTGTCTGGCGTG	ACGGG	0.6302	2	3	-	25	6	100599141
+TNMD	CRISPRko	GAACTTCCAATAAATGACTAT	GTGAG	0.7101	17	4	+	12	6	100599180
+TNMD	CRISPRko	TGTCATCATGCCTTGTAACTG	GTGGG	0.7276	22	5	+	10	7	100599684
+TNMD	CRISPRko	GGTCACGTTATCACAAATCTC	CAGAA	0.6711	14	6	-	18	5	100597609
+TNMD	CRISPRko	TCCATACTGCTACCAAGGAGG	ACGAG	0.9069	32	7	+	2	7	100599648
+TNMD	CRISPRko	GGAATAGAATTTGATCCCATG	CTGGA	0.6871	19	8	+	15	7	100599535
+TNMD	CRISPRko	CAGGACAATTAGAGTTAGGGC	CAGGA	0.6637	18	9	-	20	2	100585303
+TNMD	CRISPRko	AGGGATTGAACAAAATGAACA	GTGGG	0.9536	38	10	+	1	6	100599098
+TNMD	CRISPRko	GACAGTCTTCACAATTCTCTG	GAGGA	0.6991	31	11	-	13	1	100585038
+TNMD	CRISPRko	CTTTCCTGCCAACGAAAAAAA	AGGGA	0.7332	37	12	+	9	6	100599077
+TNMD	CRISPRko	CTCGTCCTCCTTGGTAGCAGT	ATGGA	0.6228	21	13	-	29	7	100599638
+TNMD	CRISPRko	ATTTGATCCCATGCTGGATGA	GAGAG	0.6254	23	14	+	28	7	100599543
+TNMD	CRISPRko	CCCATATCCATACTGCTACCA	AGGAG	0.5647	10	15	+	42	7	100599642
+TNMD	CRISPRko	GTGGTCCCTCAAGTGAAAGTA	GAGAA	0.563	8	16	+	44	6	100599123
+TNMD	CRISPRko	GCATGCTGGGGAGGGTCTAAT	AGGAG	0.5694	15	17	+	40	7	100599716
+TNMD	CRISPRko	GGATACACTGGCATCTACTTC	GTGGG	0.877	58	18	+	3	4	100594279
+TNMD	CRISPRko	CAATAACCTCTCTCATCCAGC	ATGGG	0.5386	11	19	-	51	7	100599540
+TNMD	CRISPRko	CGTCACGCCAGACAAGCAAGT	GAGGA	0.5033	5	20	+	59	6	100599153
+TNMD	CRISPRko	ACTTTCCTGCCAACGAAAAAA	AAGGG	0.5778	26	21	+	38	6	100599076
+TNMD	CRISPRko	TTGTGATAACGTGACCATGTA	TTGGA	0.6649	46	22	+	19	5	100597629
+TNMD	CRISPRko	TCAGAAGCGGAAATGGCACTG	ATGAA	0.587	30	23	+	37	3	100594001
+TNMD	CRISPRko	CTCCTTGGTAGCAGTATGGAT	ATGGG	0.7387	61	24	-	8	7	100599632
+TNMD	CRISPRko	CCCGTCACGCCAGACAAGCAA	GTGAG	0.6368	49	25	+	22	6	100599151
+TNMD	CRISPRko	TTTCTTTGAACAGTCAGTGAT	TTGGG	0.6848	57	26	+	16	5	100597542
+TNMD	CRISPRko	AAACTCAGATTAAAGTGATTC	CTGAA	0.6183	43	27	+	31	4	100594325
+TNMD	CRISPRko	GTTACAAGACTTTGAGGAGGA	GGGAG	0.5902	39	28	+	36	6	100599041
+TNMD	CRISPRko	CCAGAACTGAAATATTCAGAA	GCGGA	0.5647	40	29	+	41	3	100593986
+TNMD	CRISPRko	AAATTGATCCTGTGACCAGAA	CTGAA	0.5471	35	30	+	46	3	100593971
+TNMD	CRISPRko	ACTTTTTTGGGTACCTCCGGC	CAGAA	0.4495	1	31	-	80	2	100585347
+TNMD	CRISPRko	ACCATGGCAAAGAATCCTCCA	GAGAA	0.7573	77	32	+	5	1	100585034
+TNMD	CRISPRko	GTGGGTGGCCCGCATGCTGGG	GAGGG	0.7412	82	33	+	7	7	100599705
+TNMD	CRISPRko	GTCACGCCAGACAAGCAAGTG	AGGAA	0.4853	24	34	+	65	6	100599154
+TNMD	CRISPRko	TTATTGTTGTATTTACTGCCG	TCGAG	0.439	3	35	+	87	7	100599570
+TNMD	CRISPRko	TCATCAGTGCCATTTCCGCTT	CTGAA	0.6002	56	36	-	35	3	100593990
+TNMD	CRISPRko	AGACCATGGCAAAGAATCCTC	CAGAG	0.5455	48	37	+	48	1	100585032
+TNMD	CRISPRko	CTATTAGACCCTCCCCAGCAT	GCGGG	0.4407	13	38	-	84	7	100599703
+TNMD	CRISPRko	GTGTGCTGAGAGACCATGGCA	AAGAA	0.7511	91	39	+	6	1	100585022
+TNMD	CRISPRko	AAATTCAGGAATCACTTTAAT	CTGAG	0.6198	69	40	-	30	4	100594318
+TNMD	CRISPRko	TTCTTAAAAATTCCAAAATTC	TGGAG	0.4973	42	41	+	62	5	100597601
+TNMD	CRISPRko	GAATTTGATCCCATGCTGGAT	GAGAG	0.4229	7	42	+	99	7	100599541
+TNMD	CRISPRko	GAAATGGCACTGATGAAACAT	TGGAA	0.4507	28	43	+	79	3	100594010
+TNMD	CRISPRko	CTGCCAACGAAAAAAAAGGGA	TTGAA	0.4866	44	44	+	64	6	100599082
+TNMD	CRISPRko	CTCTTCTGGTTCAGAAAATTC	AGGAA	0.6963	97	45	-	14	4	100594333
+TNMD	CRISPRko	CCTGAATTTTCTGAACCAGAA	GAGGA	0.5461	68	46	+	47	4	100594345
+TNMD	CRISPRko	TATCACAAATCTCCAGAATTT	TGGAA	0.5434	67	47	-	50	5	100597601
+TNMD	CRISPRko	TAGTTTCTGAGTTACAAGACT	TTGAG	0.5289	64	48	+	55	6	100599031
+TNMD	CRISPRko	CATATCCATACTGCTACCAAG	GAGGA	0.4119	20	49	+	103	7	100599644
+TNMD	CRISPRko	TTGCATTTATTATCTTAGTTT	CTGAG	0.6603	103	50	+	21	6	100599016
+TNMD	CRISPRko	AATGTCATACCTGATATTAGA	GTGGG	0.5521	79	51	-	45	5	100597650
+TNMD	CRISPRko	ATAAAACATTTTTGAAGACCC	ACGAA	0.5137	66	52	-	58	4	100594287
+TNMD	CRISPRko	AAATATGAATCAACTTACATT	TAGAA	0.6137	90	53	-	34	1	100585067
+TNMD	CRISPRko	CCAAAACAGGACAATTAGAGT	TAGGG	0.4356	34	54	-	91	2	100585309
+TNMD	CRISPRko	CGCATGCTGGGGAGGGTCTAA	TAGGA	0.3955	16	55	+	110	7	100599715
+TNMD	CRISPRko	GAACAGTGGGTGGTCCCTCAA	GTGAA	0.4555	52	56	+	76	6	100599114
+TNMD	CRISPRko	GTTTCTGAGTTACAAGACTTT	GAGGA	0.4695	62	57	+	70	6	100599033
+TNMD	CRISPRko	GGAAATGGCACTGATGAAACA	TTGGA	0.4047	25	58	+	107	3	100594009
+TNMD	CRISPRko	AATAACCTCTCTCATCCAGCA	TGGGA	0.6257	106	59	-	27	7	100599539
+TNMD	CRISPRko	AATCCCTTTTTTTTCGTTGGC	AGGAA	0.3187	4	60	-	129	6	100599069
+TNMD	CRISPRko	ACCCATATCCATACTGCTACC	AAGGA	0.3248	6	61	+	128	7	100599641
+TNMD	CRISPRko	TGAATATTTCAGTTCTGGTCA	CAGGA	0.6257	111	62	-	26	3	100593968
+TNMD	CRISPRko	AGCAGAAAAGCCTATTGAAAA	CCGAG	0.6302	114	63	+	24	5	100597572
+TNMD	CRISPRko	TGTGACCAGAACTGAAATATT	CAGAA	0.4611	65	64	+	73	3	100593981
+TNMD	CRISPRko	AGCACACTTTCTACAGCAATG	GAGAG	0.538	86	65	+	52	3	100593926
+TNMD	CRISPRko	TGGAGCACACTTTCTACAGCA	ATGGA	0.4467	59	66	+	82	3	100593923
+TNMD	CRISPRko	TGTGCTCCATGTCATAGGCCT	GGGAG	0.4844	76	67	-	66	3	100593896
+TNMD	CRISPRko	ACTGGTGGGTGGCCCGCATGC	TGGGG	0.4272	47	68	+	96	7	100599701
+TNMD	CRISPRko	ACTTTCTACAGCAATGGAGAG	AAGAA	0.5636	100	69	+	43	3	100593931
+TNMD	CRISPRko	GAGTTACAAGACTTTGAGGAG	GAGGG	0.4628	72	70	+	72	6	100599039
+TNMD	CRISPRko	ACCCAAATCACTGACTGTTCA	AAGAA	0.3649	29	71	-	116	5	100597533
+TNMD	CRISPRko	TTTCTGAGTTACAAGACTTTG	AGGAG	0.5283	89	72	+	57	6	100599034
+TNMD	CRISPRko	GATGTGTATTTACTTACTTTT	TTGGG	0.6755	129	73	-	17	2	100585362
+TNMD	CRISPRko	TAGAATTTGATCCCATGCTGG	ATGAG	0.4801	80	74	+	68	7	100599539
+TNMD	CRISPRko	TGTTCTCCCAGGCCTATGACA	TGGAG	0.4303	53	75	+	95	3	100593902
+TNMD	CRISPRko	CAGAACTGAAATATTCAGAAG	CGGAA	0.46	75	76	+	74	3	100593987
+TNMD	CRISPRko	CATGGAAATTGATCCTGTGAC	CAGAA	0.4315	55	77	+	94	3	100593966
+TNMD	CRISPRko	TCTTTCAGACTGAAAATGGAA	TAGAA	0.6313	127	78	+	23	7	100599518
+TNMD	CRISPRko	AACTGGTGGGTGGCCCGCATG	CTGGG	0.4077	50	79	+	104	7	100599700
+TNMD	CRISPRko	AAAAAAAAGGGATTGAACAAA	ATGAA	0.6157	123	80	+	32	6	100599091
+TNMD	CRISPRko	CACACTTTCTACAGCAATGGA	GAGAA	0.5437	108	81	+	49	3	100593928
+TNMD	CRISPRko	TTCCTGAATTTTCTGAACCAG	AAGAG	0.4635	87	82	+	71	4	100594343
+TNMD	CRISPRko	CCTAACTCTAATTGTCCTGTT	TTGGG	0.3066	27	83	+	131	2	100585320
+TNMD	CRISPRko	ATGTCATACCTGATATTAGAG	TGGGA	0.5327	105	84	-	54	5	100597649
+TNMD	CRISPRko	AGTGTGCTCCATGTCATAGGC	CTGGG	0.4017	51	85	-	108	3	100593898
+TNMD	CRISPRko	ACCGCTATTGCCGCCGCGTCT	GTGAA	0.366	45	86	+	115	7	100599599
+TNMD	CRISPRko	CTGAACCAGAAGAGGAAATAG	ATGAG	0.5765	124	87	+	39	4	100594355
+TNMD	CRISPRko	GTGTGCTCCATGTCATAGGCC	TGGGA	0.4404	78	88	-	85	3	100593897
+TNMD	CRISPRko	CTTCTTCTTTCAGACTGAAAA	TGGAA	0.614	131	89	+	33	7	100599513
+TNMD	CRISPRko	CTGTTCTCCCAGGCCTATGAC	ATGGA	0.4062	63	90	+	105	3	100593901
+TNMD	CRISPRko	GAAGTGCTTGCTCCCCCAAAA	CAGGA	0.4366	81	91	-	90	2	100585324
+TNMD	CRISPRko	CATAACTCACATAGTCATTTA	TTGGA	0.2678	33	92	-	139	6	100599175
+TNMD	CRISPRko	ATAACTCACATAGTCATTTAT	TGGAA	0.2723	36	93	-	138	6	100599174
+TNMD	CRISPRko	ACGCCAGACAAGCAAGTGAGG	AAGAA	0.4468	95	94	+	81	6	100599157
+TNMD	CRISPRko	AGTTACAAGACTTTGAGGAGG	AGGGA	0.4517	99	95	+	77	6	100599040
+TNMD	CRISPRko	GGTTTTCAATAGGCTTTTCTG	CTGGG	0.4161	74	96	-	102	5	100597559
+TNMD	CRISPRko	GGTCTTCTCTACTTTCACTTG	AGGGA	0.3572	60	97	-	117	6	100599117
+TNMD	CRISPRko	CAAAGAATCCTCCAGAGAATT	GTGAA	0.4841	110	98	+	67	1	100585041
+TNMD	CRISPRko	GGGTCTTCTCTACTTTCACTT	GAGGG	0.5022	119	99	-	60	6	100599118
+TNMD	CRISPRko	CTGAATTTTCTGAACCAGAAG	AGGAA	0.4974	120	100	+	61	4	100594346
+TNMD	CRISPRko	TTTCTTAAAAATTCCAAAATT	CTGGA	0.149	41	101	+	143	5	100597600
+TNMD	CRISPRko	GTGACAGTCTTCACAATTCTC	TGGAG	0.4191	84	102	-	100	1	100585040
+TNMD	CRISPRko	GAGAGAAGAAGAAGATTTACA	TGGAA	0.5338	133	103	+	53	3	100593947
+TNMD	CRISPRko	TATGTAAATCACTTAAGATTT	GTGGA	0.3764	73	104	+	113	2	100585274
+TNMD	CRISPRko	TTAAAGTGATTCCTGAATTTT	CTGAA	0.3526	71	105	+	119	4	100594334
+TNMD	CRISPRko	ACTCTAATTGTCCTGTTTTGG	GGGAG	0.4266	96	106	+	97	2	100585324
+TNMD	CRISPRko	GGGTGGTCCCTCAAGTGAAAG	TAGAG	0.439	107	107	+	88	6	100599121
+TNMD	CRISPRko	CAATCCCTTTTTTTTCGTTGG	CAGGA	0.2624	54	108	-	141	6	100599070
+TNMD	CRISPRko	CTGAGTTACAAGACTTTGAGG	AGGAG	0.4408	112	109	+	83	6	100599037
+TNMD	CRISPRko	CCTCTTCTGGTTCAGAAAATT	CAGGA	0.3353	70	110	-	125	4	100594334
+TNMD	CRISPRko	TACAAGACTTTGAGGAGGAGG	GAGAA	0.5287	140	111	+	56	6	100599043
+TNMD	CRISPRko	GGGTCCCAGCAGAAAAGCCTA	TTGAA	0.3945	85	112	+	111	5	100597565
+TNMD	CRISPRko	AAGATCTTCACTTTCCTGCCA	ACGAA	0.4057	92	113	+	106	6	100599067
+TNMD	CRISPRko	TCTGAGTTACAAGACTTTGAG	GAGGA	0.4798	130	114	+	69	6	100599036
+TNMD	CRISPRko	TTCTACAGCAATGGAGAGAAG	AAGAA	0.494	136	115	+	63	3	100593934
+TNMD	CRISPRko	TTTTTTTTCGTTGGCAGGAAA	GTGAA	0.4374	113	116	-	89	6	100599063
+TNMD	CRISPRko	CCTCCTCAAAGTCTTGTAACT	CAGAA	0.3518	83	117	-	120	6	100599025
+TNMD	CRISPRko	GTTTTCAATAGGCTTTTCTGC	TGGGA	0.4517	125	118	-	78	5	100597558
+TNMD	CRISPRko	TAGGCAGAAGCTTTTAAATCC	AAGAA	0.3538	88	119	+	118	2	100585246
+TNMD	CRISPRko	GGGGGAGCAAGCACTTCTGGC	CGGAG	0.4341	117	120	+	93	2	100585343
+TNMD	CRISPRko	TTAAGTGATTTACATATTTTC	TTGGA	0.3969	101	121	-	109	2	100585254
+TNMD	CRISPRko	GGAGAGAAGAAGAAGATTTAC	ATGGA	0.4598	138	122	+	75	3	100593946
+TNMD	CRISPRko	GGAGCACACTTTCTACAGCAA	TGGAG	0.4174	116	123	+	101	3	100593924
+TNMD	CRISPRko	TGTGACAGTCTTCACAATTCT	CTGGA	0.3347	94	124	-	126	1	100585041
+TNMD	CRISPRko	AACTCTAATTGTCCTGTTTTG	GGGGA	0.4246	126	125	+	98	2	100585323
+TNMD	CRISPRko	ACGGGTCTTCTCTACTTTCAC	TTGAG	0.4403	142	126	-	86	6	100599120
+TNMD	CRISPRko	AAGAAATTACCACAACTTTCT	TTGAA	0.2727	93	127	+	137	5	100597526
+TNMD	CRISPRko	TTATCACAAATCTCCAGAATT	TTGGA	0.2989	98	128	-	134	5	100597602
+TNMD	CRISPRko	AGTCAGTGATTTGGGTCCCAG	CAGAA	0.4346	141	129	+	92	5	100597553
+TNMD	CRISPRko	TGATTCCTGAATTTTCTGAAC	CAGAA	0.3715	122	130	+	114	4	100594340
+TNMD	CRISPRko	TGGTGGGTGGCCCGCATGCTG	GGGAG	0.3	104	131	+	133	7	100599703
+TNMD	CRISPRko	CTGAGTTTTGATAAAACATTT	TTGAA	0.2768	102	132	-	136	4	100594297
+TNMD	CRISPRko	TGGGGGAGCAAGCACTTCTGG	CCGGA	0.3923	128	133	+	112	2	100585342
+TNMD	CRISPRko	CTGGTGGGTGGCCCGCATGCT	GGGGA	0.3306	115	134	+	127	7	100599702
+TNMD	CRISPRko	CTAACTCTAATTGTCCTGTTT	TGGGG	0.2943	109	135	+	135	2	100585321
+TNMD	CRISPRko	TTCTTCTTCTCTCCATTGCTG	TAGAA	0.305	118	136	-	132	3	100593924
+TNMD	CRISPRko	AGTTGTGGTAATTTCTTCATT	CTGAA	0.3146	121	137	-	130	5	100597507
+TNMD	CRISPRko	AAATTCTATTCCATTTTCAGT	CTGAA	0.3443	134	138	-	122	7	100599511
+TNMD	CRISPRko	TAACTCTAATTGTCCTGTTTT	GGGGG	0.3376	132	139	+	124	2	100585322
+TNMD	CRISPRko	TCTTCTTCTTTCAGACTGAAA	ATGGA	0.3484	137	140	+	121	7	100599512
+TNMD	CRISPRko	CTACTTTCTTCTTTCAGAATG	AAGAA	0.3386	139	141	+	123	5	100597505
+TNMD	CRISPRko	CATCTATTTCCTCTTCTGGTT	CAGAA	0.2584	135	142	-	142	4	100594343
+TNMD	CRISPRko	CTCCAGAATTTTGGAATTTTT	AAGAA	0.2656	143	143	-	140	5	100597591
+DPM1	CRISPRko	TAGTACCTACCTAATAAATTC	AGGAA	0.6898	7	1	+	10	5	50945745
+DPM1	CRISPRko	CGTTGTAGGTAGGTAAAAGCA	CCGAA	0.7132	12	2	+	7	1	50958436
+DPM1	CRISPRko	GGAGAAGATCTATGGGTCAGA	CAGAA	0.6433	24	3	-	14	2	50955196
+DPM1	CRISPRko	TTACCTACCTACAACGAGCGC	GAGAA	0.5488	1	4	-	37	1	50958416
+DPM1	CRISPRko	TCCTGTGGCATGTTTCATTCC	ATGAA	0.6405	25	5	+	15	4	50945904
+DPM1	CRISPRko	CTACAGCTTCTAAGACCACGA	GAGAA	0.5438	5	6	-	39	3	50948651
+DPM1	CRISPRko	GAGGACTACGACTGACTTCCA	AGGAG	0.5354	4	7	+	41	1	50958511
+DPM1	CRISPRko	CTGAACAGTTGGAGAAGATCT	ATGGG	0.8405	45	8	-	1	2	50955206
+DPM1	CRISPRko	TTTTACCTACCTACAACGAGC	GCGAG	0.5178	2	9	-	44	1	50958418
+DPM1	CRISPRko	GTAGTCCTCGCAGGTCTCGGC	GGGAG	0.5657	15	10	-	32	1	50958484
+DPM1	CRISPRko	TTTAGGAACTGCATATATTCA	TGGAA	0.6201	30	11	-	18	4	50945911
+DPM1	CRISPRko	TCATGGAATGAAACATGCCAC	AGGAA	0.5846	20	12	-	29	4	50945893
+DPM1	CRISPRko	AACATACCACTTACATGGTGT	GAGAG	0.6482	37	13	+	13	4	50945851
+DPM1	CRISPRko	ACAGGAAACTACATCATTATT	ATGGA	0.5965	27	14	-	23	4	50945874
+DPM1	CRISPRko	CTGGAAGTGCGCAGTCCACGA	CAGAA	0.4861	3	15	-	52	1	50958458
+DPM1	CRISPRko	AATTTCAGCCAAAATTTATTC	CTGAA	0.589	31	16	-	28	5	50945753
+DPM1	CRISPRko	GATGGAAGCCCAGATGGAACA	AGGGA	0.6985	52	17	-	8	2	50955237
+DPM1	CRISPRko	TCCTACAGCTTCTAAGACCAC	GAGAG	0.505	17	18	-	48	3	50948653
+DPM1	CRISPRko	ATTCCTACAGCTTCTAAGACC	ACGAG	0.4741	8	19	-	57	3	50948655
+DPM1	CRISPRko	GGCTGCTGGTGAAAAGCTTCT	CCGAG	0.592	39	20	-	26	1	50958373
+DPM1	CRISPRko	CTAAGACCACGAGAGAAAAAG	TTGGG	0.6165	48	21	-	19	3	50948642
+DPM1	CRISPRko	TGATGGAAGCCCAGATGGAAC	AAGGG	0.5641	36	22	-	34	2	50955238
+DPM1	CRISPRko	CTGCTGGTGAAAAGCTTCTCC	GAGAG	0.6271	54	23	-	16	1	50958371
+DPM1	CRISPRko	AGGGATGTTGCTGAACAGTTG	GAGAA	0.5105	26	24	-	45	2	50955216
+DPM1	CRISPRko	TCATAGATGATGGAAGCCCAG	ATGGA	0.606	51	25	-	21	2	50955245
+DPM1	CRISPRko	TGCTCCTGGTCTCAGCAAGAT	CTGAG	0.7799	74	26	+	3	7	50940906
+DPM1	CRISPRko	AACTAGGTTCCAATATCATTT	GTGGA	0.6946	72	27	-	9	9	50935225
+DPM1	CRISPRko	GACCTGGTGCGCTACCTCTCG	GAGAA	0.5024	33	28	+	49	1	50958366
+DPM1	CRISPRko	CATAGATGATGGAAGCCCAGA	TGGAA	0.576	53	29	-	30	2	50955244
+DPM1	CRISPRko	CTGACTTCCAAGGAGGCCATG	GCGGA	0.5926	59	30	+	25	1	50958522
+DPM1	CRISPRko	ATGATTGTTCGGGCAAGACAG	TTGAA	0.7444	81	31	-	4	8	50936172
+DPM1	CRISPRko	GCACCGAATATTTGTTCTGTC	GTGGA	0.4512	22	32	+	64	1	50958454
+DPM1	CRISPRko	CAGATGGAACAAGGGATGTTG	CTGAA	0.4145	9	33	-	78	2	50955227
+DPM1	CRISPRko	CGAGGACTACGACTGACTTCC	AAGGA	0.4226	14	34	+	73	1	50958510
+DPM1	CRISPRko	ACTGCGCACTTCCAGCTCCCG	CCGAG	0.5467	50	35	+	38	1	50958479
+DPM1	CRISPRko	CCAGCTCCCGCCGAGACCTGC	GAGGA	0.5732	58	36	+	31	1	50958490
+DPM1	CRISPRko	TAAGACCACGAGAGAAAAAGT	TGGGA	0.4415	21	37	-	69	3	50948641
+DPM1	CRISPRko	CAAGGGATGTTGCTGAACAGT	TGGAG	0.4566	28	38	-	62	2	50955218
+DPM1	CRISPRko	TGACTTCCAAGGAGGCCATGG	CGGAA	0.5645	57	39	+	33	1	50958523
+DPM1	CRISPRko	TTCCAGCTCCCGCCGAGACCT	GCGAG	0.5053	44	40	+	47	1	50958488
+DPM1	CRISPRko	GAAACATACCACTTACATGGT	GTGAG	0.4321	23	41	+	70	4	50945849
+DPM1	CRISPRko	TACACCTCCATTTCCTTTGTA	GCGAG	0.8036	92	42	+	2	6	50942081
+DPM1	CRISPRko	TCGTAGTCCTCGCAGGTCTCG	GCGGG	0.4805	42	43	-	54	1	50958486
+DPM1	CRISPRko	CTCTCGTGGTCTTAGAAGCTG	TAGGA	0.3825	6	44	+	91	3	50948663
+DPM1	CRISPRko	TTTCTTTTATGTAGTAGCAAA	AAGAG	0.7369	93	45	+	6	9	50935144
+DPM1	CRISPRko	CTAAAGGCTACGTCTTCCAGA	TGGAG	0.6227	82	46	-	17	8	50936198
+DPM1	CRISPRko	CGTAGTCCTCGCAGGTCTCGG	CGGGA	0.4045	16	47	-	83	1	50958485
+DPM1	CRISPRko	GGAAATGGAGGTGTATATGGC	TGGGA	0.6012	80	48	-	22	6	50942060
+DPM1	CRISPRko	TTATTCTACAGGAAGCAAAAG	GAGGG	0.7439	97	49	-	5	6	50942120
+DPM1	CRISPRko	GAGACCTGGTGCGCTACCTCT	CGGAG	0.4427	34	50	+	68	1	50958364
+DPM1	CRISPRko	TTCATGGAATGAAACATGCCA	CAGGA	0.4511	38	51	-	65	4	50945894
+DPM1	CRISPRko	CTCGCAGGTCTCGGCGGGAGC	TGGAA	0.3984	18	52	-	85	1	50958478
+DPM1	CRISPRko	GATTATACCGAAAAGAAGTTC	TAGAG	0.6149	84	53	-	20	8	50936246
+DPM1	CRISPRko	CTTTTCACCAGCAGCCACACG	ATGAG	0.4595	43	54	+	61	1	50958393
+DPM1	CRISPRko	CATTTCCTTTGTAGCGAGTTC	CAGAG	0.5355	65	55	+	40	6	50942089
+DPM1	CRISPRko	AAATTCAGGAATAAATTTTGG	CTGAA	0.4168	32	56	+	75	5	50945760
+DPM1	CRISPRko	CAACTTTTTCTCTCGTGGTCT	TAGAA	0.3735	11	57	+	96	3	50948654
+DPM1	CRISPRko	ACAAGGGATGTTGCTGAACAG	TTGGA	0.4521	46	58	-	63	2	50955219
+DPM1	CRISPRko	GGAACTGCATATATTCATGGA	ATGAA	0.3731	13	59	-	97	4	50945907
+DPM1	CRISPRko	CAGCAACATCCCTTGTTCCAT	CTGGG	0.3786	19	60	+	93	2	50955239
+DPM1	CRISPRko	TGTTTATGGTGAATCCAAGTT	GGGAG	0.5956	89	61	-	24	9	50935196
+DPM1	CRISPRko	CATTTGTGGATCGTGTTTATG	GTGAA	0.6662	101	62	-	12	9	50935209
+DPM1	CRISPRko	TTTTAGGAACTGCATATATTC	ATGGA	0.3307	10	63	-	105	4	50945912
+DPM1	CRISPRko	TGTCTCTGGAACTCGCTACAA	AGGAA	0.48	63	64	-	55	6	50942082
+DPM1	CRISPRko	ATGAAATAGTATCTTTCTTGA	AAGGA	0.6731	108	65	-	11	9	50935167
+DPM1	CRISPRko	CGGTGCTTTTACCTACCTACA	ACGAG	0.3935	35	66	-	87	1	50958424
+DPM1	CRISPRko	GGAGACCTGGTGCGCTACCTC	TCGGA	0.4209	49	67	+	74	1	50958363
+DPM1	CRISPRko	ACTATTTCATTTCCTCCCAAC	TTGGA	0.5601	91	68	+	35	9	50935193
+DPM1	CRISPRko	AAACACGATCCACAAATGATA	TTGGA	0.4796	70	69	+	56	9	50935227
+DPM1	CRISPRko	CCATCTGGAAGACGTAGCCTT	TAGAA	0.4596	66	70	+	60	8	50936210
+DPM1	CRISPRko	AACACGATCCACAAATGATAT	TGGAA	0.4636	68	71	+	59	9	50935228
+DPM1	CRISPRko	GACAGTTGAATTATACTATTG	GCGAG	0.5223	85	72	-	43	8	50936156
+DPM1	CRISPRko	TTATACCGAAAAGAAGTTCTA	GAGAA	0.4705	76	73	-	58	8	50936244
+DPM1	CRISPRko	TCAGTTCCGCCATGGCCTCCT	TGGAA	0.4101	55	74	-	79	1	50958517
+DPM1	CRISPRko	TGGAACTCGCTACAAAGGAAA	TGGAG	0.5353	94	75	-	42	6	50942076
+DPM1	CRISPRko	CGTGTTTATGGTGAATCCAAG	TTGGG	0.4825	86	76	-	53	9	50935198
+DPM1	CRISPRko	ACCAGGAGCATCTGATTTAAC	AGGAA	0.451	73	77	-	66	7	50940880
+DPM1	CRISPRko	TTACCCTCCTTTTGCTTCCTG	TAGAA	0.5917	113	78	+	27	6	50942127
+DPM1	CRISPRko	TTATGGTGAATCCAAGTTGGG	AGGAA	0.4455	75	79	-	67	9	50935193
+DPM1	CRISPRko	ATAGTACCTACCTAATAAATT	CAGGA	0.2776	29	80	+	113	5	50945744
+DPM1	CRISPRko	TTGTCTCTGGAACTCGCTACA	AAGGA	0.4099	64	81	-	80	6	50942083
+DPM1	CRISPRko	TGAAATTATAATCATAGATGA	TGGAA	0.3495	41	82	-	103	2	50955256
+DPM1	CRISPRko	CCTCGCAGGTCTCGGCGGGAG	CTGGA	0.3878	56	83	-	90	1	50958479
+DPM1	CRISPRko	ATTTATTCTACAGGAAGCAAA	AGGAG	0.5511	112	84	-	36	6	50942122
+DPM1	CRISPRko	ATTTTTTTAGTGGAATCAACT	ATGAA	0.2968	40	85	-	109	2	50955278
+DPM1	CRISPRko	GATTGTTTTATTTGGCAGCCG	TGGGG	0.426	79	86	-	72	7	50940934
+DPM1	CRISPRko	AACTCAGATCTTGCTGAGACC	AGGAG	0.4862	102	87	-	51	7	50940898
+DPM1	CRISPRko	AATTTTTTAACTCAGATCTTG	CTGAG	0.4875	106	88	-	50	7	50940906
+DPM1	CRISPRko	AGTATAATTCAACTGTCTTGC	CCGAA	0.3982	71	89	+	86	8	50936175
+DPM1	CRISPRko	GTGTTTATGGTGAATCCAAGT	TGGGA	0.427	88	90	-	71	9	50935197
+DPM1	CRISPRko	TAACTCAGATCTTGCTGAGAC	CAGGA	0.4156	83	91	-	76	7	50940899
+DPM1	CRISPRko	CTTCCAGATGGAGATGATTGT	TCGGG	0.5086	114	92	-	46	8	50936185
+DPM1	CRISPRko	ACTCTTTTTGCTACTACATAA	AAGAA	0.4014	77	93	-	84	9	50935135
+DPM1	CRISPRko	ATGAAATTATAATCATAGATG	ATGGA	0.2538	47	94	-	114	2	50955257
+DPM1	CRISPRko	CTCAGTTCCGCCATGGCCTCC	TTGGA	0.3633	61	95	-	101	1	50958518
+DPM1	CRISPRko	CCGCTCATCGTGTGGCTGCTG	GTGAA	0.3101	60	96	-	106	1	50958386
+DPM1	CRISPRko	TTTATGGTGAATCCAAGTTGG	GAGGA	0.3686	67	97	-	100	9	50935194
+DPM1	CRISPRko	TGCCCGAACAATCATCTCCAT	CTGGA	0.3447	69	98	+	104	8	50936193
+DPM1	CRISPRko	TTTCTTACAGATTATACCGAA	AAGAA	0.2806	62	99	-	112	8	50936255
+DPM1	CRISPRko	GGAGGTGTATATGGCTGGGAT	TTGAA	0.393	90	100	-	88	6	50942054
+DPM1	CRISPRko	GACCAGGAGCATCTGATTTAA	CAGGA	0.3797	87	101	-	92	7	50940881
+DPM1	CRISPRko	GCCCGAACAATCATCTCCATC	TGGAA	0.4059	99	102	+	82	8	50936194
+DPM1	CRISPRko	AGGAAATGGAGGTGTATATGG	CTGGG	0.4075	105	103	-	81	6	50942061
+DPM1	CRISPRko	GTGAATCCAAGTTGGGAGGAA	ATGAA	0.4151	110	104	-	77	9	50935188
+DPM1	CRISPRko	GGGTAATTTTGATATTGTCTC	TGGAA	0.2838	78	105	-	111	6	50942097
+DPM1	CRISPRko	TGTATATGGCTGGGATTTGAA	AAGAA	0.3739	98	106	-	95	6	50942049
+DPM1	CRISPRko	AATTTATTCTACAGGAAGCAA	AAGGA	0.389	107	107	-	89	6	50942123
+DPM1	CRISPRko	AAAAGAGTCAATAATCCTTTC	AAGAA	0.3759	104	108	+	94	9	50935163
+DPM1	CRISPRko	TCTAAAGGCTACGTCTTCCAG	ATGGA	0.3719	100	109	-	98	8	50936199
+DPM1	CRISPRko	CTGGAACTCGCTACAAAGGAA	ATGGA	0.3698	103	110	-	99	6	50942077
+DPM1	CRISPRko	TTTAATTTCTTACAGATTATA	CCGAA	0.2985	95	111	-	108	8	50936260
+DPM1	CRISPRko	AGGGTAATTTTGATATTGTCT	CTGGA	0.3047	96	112	-	107	6	50942098
+DPM1	CRISPRko	GGAAATGAAATAGTATCTTTC	TTGAA	0.3562	115	113	-	102	9	50935171
+DPM1	CRISPRko	AAGAAGTTCTAGAGAAATTAA	TAGAA	0.2933	109	114	-	110	8	50936234
+DPM1	CRISPRko	ACATTTTTCTATTAATTTCTC	TAGAA	0.1536	111	115	+	115	8	50936238
+SCYL3	CRISPRko	CTCCAGAATTCACAACTCTCC	CAGAG	0.9182	34	1	-	3	6	169870339
+SCYL3	CRISPRko	CTGTACTCGCTCAGTGACAAG	ATGAA	0.6959	16	2	+	39	3	169878745
+SCYL3	CRISPRko	GCAGAGCGCTGGCCGACATTT	TGGAA	0.6738	8	3	+	51	7	169868972
+SCYL3	CRISPRko	ATCTGCTTGGCCCCAAAAAAG	GTGAA	0.7725	46	4	-	21	9	169864372
+SCYL3	CRISPRko	AAGCACGGGGATCACCCGTGA	CTGGA	0.6221	5	5	+	63	10	169862745
+SCYL3	CRISPRko	GTCAAGTCCCAGTGCACTACC	TTGGA	0.6362	11	6	-	59	12	169854756
+SCYL3	CRISPRko	TTTTCGGGTAGATCATGCGCA	GGGAG	0.6008	3	7	-	69	10	169862791
+SCYL3	CRISPRko	AGATGCTGGGTCTCTTATTGA	CTGAA	0.6664	19	8	+	53	5	169873732
+SCYL3	CRISPRko	TGGCATTCATCTTGTCACTGA	GCGAG	0.8397	63	9	-	9	3	169878740
+SCYL3	CRISPRko	CTTAGATACTAAAGTAAACCC	AGGAG	0.6853	31	10	-	44	12	169854691
+SCYL3	CRISPRko	CTTTGTACAAGGCTAATCTTT	TGGGG	0.6911	38	11	+	42	12	169854574
+SCYL3	CRISPRko	GGCTCTTATCTTCCTTCATGA	CAGAG	0.8423	78	12	-	8	3	169878641
+SCYL3	CRISPRko	TATCAGCAAACCAATCCATCT	CAGGA	0.6742	40	13	+	50	12	169854422
+SCYL3	CRISPRko	TGTCCTCAGATTTCTGCTGGA	CAGAG	0.7674	68	14	-	23	9	169864500
+SCYL3	CRISPRko	GGGGGGATGACGCAGACCAAA	TCGAG	0.5747	12	15	-	80	12	169854539
+SCYL3	CRISPRko	CTGTCTCACATCGAGGCCTAC	GTGGA	0.5695	14	16	-	83	10	169862664
+SCYL3	CRISPRko	TATGCAGAGTAATTGCCACAA	TGGAA	0.8391	102	17	+	10	11	169859176
+SCYL3	CRISPRko	ACAGAGTCAGCTGCTTGTCAG	AGGAA	0.6851	67	18	-	45	9	169864480
+SCYL3	CRISPRko	TTGCTCACAATCTTAAATGAA	CAGGG	0.6788	69	19	-	48	6	169870262
+SCYL3	CRISPRko	AAGATGGACACTGGAAGCTAG	GAGGA	0.651	66	20	-	55	4	169876025
+SCYL3	CRISPRko	TTGTAGCAGTGATTCCACCTC	CTGGG	0.8014	108	21	+	15	12	169854683
+SCYL3	CRISPRko	GACCAGCACTGCTAGGCTATG	CAGAG	0.7517	99	22	+	25	11	169859159
+SCYL3	CRISPRko	TGGCCTGACTGGAGTGAACCT	GAGGA	0.5395	23	23	-	102	12	169854837
+SCYL3	CRISPRko	CCTACGTGGAGCACTTCACTC	AGGAG	0.5126	10	24	-	120	10	169862648
+SCYL3	CRISPRko	GCGGGATAAACAGCAAGTCCA	GAGGG	0.9164	128	25	+	4	2	169888773
+SCYL3	CRISPRko	CATCAGAACTTGGTTTAGGAG	AGGAA	0.6435	75	26	-	58	12	169854473
+SCYL3	CRISPRko	ACTGACCTTTCTCTAGAAGGT	AAGAA	0.5693	49	27	-	84	11	169859042
+SCYL3	CRISPRko	GTAAAGTTTCTCAGGCCACAC	CAGAG	0.5754	56	28	-	78	4	169875986
+SCYL3	CRISPRko	ATTTGCCATCTTGCAGTACAG	CGGGA	0.5257	26	29	+	109	2	169888753
+SCYL3	CRISPRko	ATTTTTCGGGTAGATCATGCG	CAGGG	0.4969	4	30	-	132	10	169862793
+SCYL3	CRISPRko	GAAAGCTGGAGAGAACATCCG	CTGAA	0.6822	91	31	+	47	7	169869031
+SCYL3	CRISPRko	CAGGGCTGGTGAGAGCAAGCA	AGGAG	0.9478	143	32	+	1	10	169862772
+SCYL3	CRISPRko	TCTCTTCAGGAGGGATAGATG	CTGGG	0.805	132	33	+	13	5	169873716
+SCYL3	CRISPRko	CTCACCAGCCCTGTTCCAGTC	ACGGG	0.7954	130	34	-	16	10	169862749
+SCYL3	CRISPRko	AAAGTCTGAGGAGTGGCCTGA	CTGGA	0.6015	79	35	-	68	12	169854850
+SCYL3	CRISPRko	TGAAGTTCATGAAGAGCATGT	GCGGA	0.8167	135	36	-	12	10	169862698
+SCYL3	CRISPRko	TCTGAGGAGTATTCAGTCAAT	AAGAG	0.5145	35	37	-	118	5	169873733
+SCYL3	CRISPRko	GCTGCAGCAGAAATTACTGAG	GTGAG	0.8175	146	38	-	11	12	169854273
+SCYL3	CRISPRko	ATTTTGAGGAAAACTGCATCA	CTGGG	0.531	52	39	+	106	12	169854311
+SCYL3	CRISPRko	TTAATTTTCTGAACTTACTTA	AAGAA	0.7491	134	40	+	26	8	169866896
+SCYL3	CRISPRko	TTCTTGAAAAGTTTAACATTG	AAGAG	0.8482	159	41	-	7	8	169866931
+SCYL3	CRISPRko	CTTTAAAGAGCTATACACTGA	GAGAA	0.5256	59	42	-	110	2	169888804
+SCYL3	CRISPRko	AAGTCATGAGATAGTAAGGTG	CAGAG	0.5041	45	43	+	126	7	169868952
+SCYL3	CRISPRko	CATCAAGTTCTAAAAAGTCTG	AGGAG	0.721	140	44	-	32	12	169854863
+SCYL3	CRISPRko	CTATGCAGAGTAATTGCCACA	ATGGA	0.4967	42	45	+	134	11	169859175
+SCYL3	CRISPRko	CTCTCCTAAACCAAGTTCTGA	TGGAA	0.5106	58	46	+	121	12	169854486
+SCYL3	CRISPRko	CGGGCATGTCCATGACACTCT	GGGAG	0.4663	18	47	+	166	6	169870332
+SCYL3	CRISPRko	AAGAGTCATCTTGGGATGACT	GCGAG	0.5097	65	48	-	122	12	169854725
+SCYL3	CRISPRko	TCCGAAGTATTTTTTACATCT	GAGAG	0.6986	155	49	+	38	12	169854916
+SCYL3	CRISPRko	GAGACCCAGCATCTATCCCTC	CTGAA	0.4641	22	50	-	172	5	169873710
+SCYL3	CRISPRko	AAGATAAAAATCTTAGCAAGC	AAGGG	0.7059	160	51	+	35	3	169878792
+SCYL3	CRISPRko	AGCAGCAGAAGGCTTAATTTC	TGGGA	0.6115	133	52	+	65	12	169854393
+SCYL3	CRISPRko	CCCAGAGTGTCATGGACATGC	CCGGG	0.5312	93	53	-	105	6	169870320
+SCYL3	CRISPRko	AGCGCTGGCCGACATTTTGGA	ATGGG	0.5241	89	54	+	112	7	169868976
+SCYL3	CRISPRko	AAAGATTAGCCTTGTACAAAG	GGGGG	0.5231	90	55	-	113	12	169854559
+SCYL3	CRISPRko	GTCTTCTGCAGAGGTCTGTGC	TGGGA	0.6263	141	56	-	62	3	169878683
+SCYL3	CRISPRko	GCGCTGGCCGACATTTTGGAA	TGGGA	0.5518	111	57	+	95	7	169868977
+SCYL3	CRISPRko	GAATTCACCATTCAAGTAAAA	AAGAA	0.4963	71	58	-	136	12	169854450
+SCYL3	CRISPRko	GAAAGTCATCTTGCCACAGGT	CAGAG	0.4676	48	59	-	161	10	169862614
+SCYL3	CRISPRko	GTCTTCACTGCTTTTAGTCTC	CAGAA	0.555	118	60	-	91	6	169870357
+SCYL3	CRISPRko	CAGGAATAGGCTTCTGCTCCC	CTGAG	0.4655	41	61	+	169	12	169854647
+SCYL3	CRISPRko	TCACAATCTTAAATGAACAGG	GTGAG	0.7578	186	62	-	24	6	169870258
+SCYL3	CRISPRko	CAGCGGGATAAACAGCAAGTC	CAGAG	0.5181	95	63	+	116	2	169888771
+SCYL3	CRISPRko	TGTCAACATACAGATTTGGCC	TAGAG	0.4535	32	64	-	184	12	169854796
+SCYL3	CRISPRko	TGCTTCAGTTTTTGTGTATAA	GAGAG	0.4932	81	65	-	137	2	169888716
+SCYL3	CRISPRko	CCATCAGAACTTGGTTTAGGA	GAGGA	0.4897	77	66	-	143	12	169854474
+SCYL3	CRISPRko	TCAACATACAGATTTGGCCTA	GAGAA	0.5196	105	67	-	115	12	169854794
+SCYL3	CRISPRko	AAAAGAAGCCAGTAAAAGATC	CTGAG	0.7042	187	68	-	36	12	169854431
+SCYL3	CRISPRko	TTCCAAGGCATAGACTCTTCA	GTGAG	0.9265	221	69	+	2	12	169854610
+SCYL3	CRISPRko	TTCAGTGAGTGAAAGCAAAGC	AGGAA	0.7821	203	70	+	20	12	169854627
+SCYL3	CRISPRko	AAACACCAACTGATTAAGCAG	AAGAG	0.4528	37	71	+	186	9	169864442
+SCYL3	CRISPRko	CCAGAGTGTCATGGACATGCC	CGGGA	0.6486	167	72	-	56	6	169870319
+SCYL3	CRISPRko	AAGATTAGCCTTGTACAAAGG	GGGGA	0.6938	184	73	-	40	12	169854558
+SCYL3	CRISPRko	TCTTCATGAACTTCAAACAAC	TGGAG	0.4346	24	74	+	202	10	169862719
+SCYL3	CRISPRko	CCTCCGAAGTATTTTTTACAT	CTGAG	0.4671	64	75	+	164	12	169854914
+SCYL3	CRISPRko	GGATGGTGCTGCTGTCTCACA	TCGAG	0.5269	121	76	-	107	10	169862675
+SCYL3	CRISPRko	TTCAAACAACTGGAGAAGCAC	GGGGA	0.7485	201	77	+	27	10	169862730
+SCYL3	CRISPRko	GCTACAAAACCTGTTACCTCA	GGGGA	0.5146	112	78	-	117	12	169854654
+SCYL3	CRISPRko	GAGTGCAAGGTCTGTTGAAAG	CTGGA	0.4745	72	79	+	157	7	169869015
+SCYL3	CRISPRko	ACTGCATAAGGGATGGGATCA	GAGAA	0.5254	119	80	-	111	2	169888835
+SCYL3	CRISPRko	ATACTAAAGTAAACCCAGGAG	GTGGA	0.5896	158	81	-	73	12	169854686
+SCYL3	CRISPRko	CTTGGATGTGGAAGAGTCATC	TTGGG	0.5313	131	82	-	104	12	169854736
+SCYL3	CRISPRko	AGTGCACTACCTTGGATGTGG	AAGAG	0.795	222	83	-	17	12	169854746
+SCYL3	CRISPRko	TATTTCTCTGTCTAGTTTCTG	AGGAG	0.7144	206	84	-	33	5	169873750
+SCYL3	CRISPRko	AGCCGCCAAAAGTGTCATCAC	AAGAA	0.403	9	85	-	233	12	169854515
+SCYL3	CRISPRko	TACTAAAGTAAACCCAGGAGG	TGGAA	0.7418	213	86	-	30	12	169854685
+SCYL3	CRISPRko	TGCTACAAAACCTGTTACCTC	AGGGG	0.3998	13	87	-	238	12	169854655
+SCYL3	CRISPRko	AAATCTTAGCAAGCAAGGGTG	ACGAA	0.4371	53	88	+	200	3	169878799
+SCYL3	CRISPRko	TTTGGGGCCAAGCAGATAAGG	AAGAA	0.4316	50	89	+	204	9	169864391
+SCYL3	CRISPRko	CAGACCTTGCACTCAACTTTG	CTGAA	0.7094	220	90	-	34	7	169868993
+SCYL3	CRISPRko	CTGCTTTGCTTTCACTCACTG	AAGAG	0.8038	241	91	-	14	12	169854614
+SCYL3	CRISPRko	CTCATTTGCTCCTAGGTTTTG	CTGGG	0.4419	61	92	-	196	11	169859210
+SCYL3	CRISPRko	TTGGAATGGGATTCAGCAAAG	TTGAG	0.789	240	93	+	18	7	169868992
+SCYL3	CRISPRko	CTACAAAACCTGTTACCTCAG	GGGAG	0.4211	44	94	-	215	12	169854653
+SCYL3	CRISPRko	CTGCTTGGACCAGAGGTGGTT	GTGGG	0.4303	54	95	-	205	11	169859120
+SCYL3	CRISPRko	GGCATGTCCATGACACTCTGG	GAGAG	0.8925	254	96	+	5	6	169870334
+SCYL3	CRISPRko	TTCCACATCCAAGGTAGTGCA	CTGGG	0.3989	21	97	+	239	12	169854759
+SCYL3	CRISPRko	CAAGGTCTGTTGAAAGCTGGA	GAGAA	0.498	129	98	+	131	7	169869020
+SCYL3	CRISPRko	TAAGCAGAAGAGGCACCAACC	TTGAA	0.463	88	99	+	173	9	169864456
+SCYL3	CRISPRko	AGTGCTTTAAAGAGCTATACA	CTGAG	0.492	122	100	-	140	2	169888808
+SCYL3	CRISPRko	AAGCCAGTAAAAGATCCTGAG	ATGGA	0.7343	232	101	-	31	12	169854426
+SCYL3	CRISPRko	TGAAAAGTTTAACATTGAAGA	GTGAA	0.4538	80	102	-	183	8	169866927
+SCYL3	CRISPRko	AAAGTTTGCTCACAATCTTAA	ATGAA	0.4215	51	103	-	213	6	169870267
+SCYL3	CRISPRko	CCTTTGTACAAGGCTAATCTT	TTGGG	0.4109	39	104	+	226	12	169854573
+SCYL3	CRISPRko	GCCTACGTGGAGCACTTCACT	CAGGA	0.466	98	105	-	168	10	169862649
+SCYL3	CRISPRko	TGCAGAGCGCTGGCCGACATT	TTGGA	0.3838	6	106	+	260	7	169868971
+SCYL3	CRISPRko	CCAAAAGATTAGCCTTGTACA	AAGGG	0.4146	47	107	-	221	12	169854562
+SCYL3	CRISPRko	AAGGCATAGACTCTTCAGTGA	GTGAA	0.4824	120	108	+	148	12	169854614
+SCYL3	CRISPRko	GTCACTGAGCGAGTACAGCCC	CTGGA	0.4532	84	109	-	185	3	169878727
+SCYL3	CRISPRko	TGTTCCAAATGAAAAGGCATC	CCGGG	0.485	124	110	+	147	6	169870310
+SCYL3	CRISPRko	TGCAAACACCAACTGATTAAG	CAGAA	0.3927	28	111	+	246	9	169864439
+SCYL3	CRISPRko	AATTTGCCATCTTGCAGTACA	GCGGG	0.5457	177	112	+	97	2	169888752
+SCYL3	CRISPRko	GCTTTTCTTATATTACCTGAA	CTGAG	0.4997	147	113	-	129	12	169854360
+SCYL3	CRISPRko	CCCGGGCATGTCCATGACACT	CTGGG	0.3905	27	114	+	250	6	169870330
+SCYL3	CRISPRko	GTTTAACATTGAAGAGTGAAG	AGGAG	0.5907	207	115	-	72	8	169866921
+SCYL3	CRISPRko	TGCAAGGTCTGTTGAAAGCTG	GAGAG	0.5022	151	116	+	128	7	169869018
+SCYL3	CRISPRko	GTGCTCCACGTAGGCCTCGAT	GTGAG	0.6114	214	117	+	66	10	169862670
+SCYL3	CRISPRko	ACTATCTCATGACTTCTTCAG	GTGAG	0.4872	137	118	-	144	7	169868931
+SCYL3	CRISPRko	TCAGATGTAAAAAATACTTCG	GAGGA	0.3912	33	119	-	249	12	169854903
+SCYL3	CRISPRko	CAATGTTTCCAAAGCCACTTC	CAGGG	0.5609	194	120	+	88	3	169878718
+SCYL3	CRISPRko	CTCTGGGAGAGTTGTGAATTC	TGGAG	0.5585	193	121	+	90	6	169870349
+SCYL3	CRISPRko	CACGCAGGCCCAGCAAAACCT	AGGAG	0.538	181	122	+	103	11	169859212
+SCYL3	CRISPRko	AGCACGGGGATCACCCGTGAC	TGGAA	0.3532	1	123	+	283	10	169862746
+SCYL3	CRISPRko	AGATGGACACTGGAAGCTAGG	AGGAA	0.7862	265	124	-	19	4	169876024
+SCYL3	CRISPRko	TCACCCTGTTCATTTAAGATT	GTGAG	0.4033	55	125	+	231	6	169870269
+SCYL3	CRISPRko	GTTTGTGAGTGAAGATGGACA	CTGGA	0.4903	145	126	-	142	4	169876036
+SCYL3	CRISPRko	ACTCACTGAAGAGTCTATGCC	TTGGA	0.3669	17	127	-	271	12	169854601
+SCYL3	CRISPRko	GGCCTGACTGGAGTGAACCTG	AGGAG	0.5937	218	128	-	71	12	169854836
+SCYL3	CRISPRko	AAGTCTGAGGAGTGGCCTGAC	TGGAG	0.8769	283	129	-	6	12	169854849
+SCYL3	CRISPRko	CCATGACACTCTGGGAGAGTT	GTGAA	0.6613	235	130	+	54	6	169870341
+SCYL3	CRISPRko	GGATCAGAGAACAGTGCTTTA	AAGAG	0.5413	190	131	-	101	2	169888820
+SCYL3	CRISPRko	GTAAAACTTGGGGCAGTGCGT	TTGAA	0.3599	15	132	+	278	11	169859085
+SCYL3	CRISPRko	GGGGATCACCCGTGACTGGAA	CAGGG	0.4367	94	133	+	201	10	169862751
+SCYL3	CRISPRko	TTTTTTGGGGCCAAGCAGATA	AGGAA	0.4566	117	134	+	178	9	169864388
+SCYL3	CRISPRko	TGCTTTAAAGAGCTATACACT	GAGAG	0.474	139	135	-	158	2	169888806
+SCYL3	CRISPRko	ATTGGTTTGCTGATATGATCC	CAGAA	0.423	86	136	-	212	12	169854401
+SCYL3	CRISPRko	CAAAAGATTAGCCTTGTACAA	AGGGG	0.4384	100	137	-	199	12	169854561
+SCYL3	CRISPRko	CAGGCCACTCCTCAGACTTTT	TAGAA	0.396	57	138	+	242	12	169854866
+SCYL3	CRISPRko	CAACCACCTCTGGTCCAAGCA	GAGAG	0.5199	185	139	+	114	11	169859134
+SCYL3	CRISPRko	TTTTTCGGGTAGATCATGCGC	AGGGA	0.3364	2	140	-	298	10	169862792
+SCYL3	CRISPRko	ATGCCCTCACCTGAAGAAGTC	ATGAG	0.6849	255	141	+	46	7	169868936
+SCYL3	CRISPRko	ATGCCCGGGATGCCTTTTCAT	TTGGA	0.3572	20	142	-	281	6	169870303
+SCYL3	CRISPRko	AGGACAGAAATGGTCCCAAAA	AAGGA	0.6933	261	143	-	41	12	169854336
+SCYL3	CRISPRko	TTCCATCAGAACTTGGTTTAG	GAGAG	0.4295	96	144	-	206	12	169854476
+SCYL3	CRISPRko	AGCCTATTAAATTTCCCATAA	ATGGA	0.4031	70	145	-	232	12	169854932
+SCYL3	CRISPRko	AAACCTGTTACCTCAGGGGAG	CAGAA	0.4755	148	146	-	155	12	169854648
+SCYL3	CRISPRko	GCTTAGATACTAAAGTAAACC	CAGGA	0.397	62	147	-	241	12	169854692
+SCYL3	CRISPRko	GAAATTTAATAGGCTGAGAAA	ATGGA	0.7466	275	148	+	29	12	169854953
+SCYL3	CRISPRko	TTTTTATCTTGTACTGTGGAA	GCGGA	0.4566	127	149	-	179	3	169878766
+SCYL3	CRISPRko	AATGTTTCCAAAGCCACTTCC	AGGGG	0.514	188	150	+	119	3	169878719
+SCYL3	CRISPRko	GATGACTTTCTTCAGCTGCTC	CTGAG	0.7471	280	151	+	28	10	169862640
+SCYL3	CRISPRko	TGGAGAGAACATCCGCTGAAA	CTGAA	0.4922	169	152	+	139	7	169869037
+SCYL3	CRISPRko	TTTACTTTAGTATCTAAGCTG	CTGGG	0.4443	115	153	+	194	12	169854709
+SCYL3	CRISPRko	GTCTGTTTATCATCTGTGTTT	GTGAG	0.5417	211	154	-	100	4	169876053
+SCYL3	CRISPRko	TCCACATCCAAGGTAGTGCAC	TGGGA	0.3222	7	155	+	305	12	169854760
+SCYL3	CRISPRko	TGTCTCACATCGAGGCCTACG	TGGAG	0.4092	83	156	-	229	10	169862663
+SCYL3	CRISPRko	ACCCAGCATCTATCCCTCCTG	AAGAG	0.6456	256	157	-	57	5	169873707
+SCYL3	CRISPRko	TAGCTCTGGATTTCAGTTTCA	GCGGA	0.595	246	158	-	70	7	169869038
+SCYL3	CRISPRko	AACTTTCCACCAATGTTCCAA	ATGAA	0.486	173	159	+	145	6	169870297
+SCYL3	CRISPRko	TGAAGATGGACACTGGAAGCT	AGGAG	0.5719	237	160	-	81	4	169876027
+SCYL3	CRISPRko	TTTGTGAGTGAAGATGGACAC	TGGAA	0.6353	259	161	-	60	4	169876035
+SCYL3	CRISPRko	AACCACCATTTACCTTACCCT	CTGGA	0.4103	92	162	-	227	2	169888780
+SCYL3	CRISPRko	TAAAAAATACTTCGGAGGACA	GTGAA	0.4422	126	163	-	195	12	169854896
+SCYL3	CRISPRko	CCAAGTTCTGATGGAACCTTA	AGGGG	0.4782	172	164	+	150	12	169854496
+SCYL3	CRISPRko	AAAGTTTAACATTGAAGAGTG	AAGAG	0.5067	198	165	-	125	8	169866924
+SCYL3	CRISPRko	TGAAGAGTGAAGAGGAGAAAA	CGGAA	0.7722	302	166	-	22	8	169866912
+SCYL3	CRISPRko	CGTGACTGGAACAGGGCTGGT	GAGAG	0.3877	73	167	+	253	10	169862761
+SCYL3	CRISPRko	TTTTTGTGTATAAGAGAGAAA	ATGAA	0.4708	166	168	-	160	2	169888708
+SCYL3	CRISPRko	CCTCTCCTAAACCAAGTTCTG	ATGGA	0.4129	106	169	+	222	12	169854485
+SCYL3	CRISPRko	TTTGCAGAGCCAGTGGCTGTT	AAGAG	0.6856	285	170	-	43	9	169864409
+SCYL3	CRISPRko	TGCCCGGGATGCCTTTTCATT	TGGAA	0.3435	36	171	-	293	6	169870302
+SCYL3	CRISPRko	ATTATTGTGTGTTAGGTGTCC	CTGGA	0.3223	25	172	+	304	4	169876089
+SCYL3	CRISPRko	GTGAAGATGGACACTGGAAGC	TAGGA	0.4554	149	173	-	181	4	169876028
+SCYL3	CRISPRko	TTGAGGAAAACTGCATCACTG	GGGAG	0.5452	234	174	+	98	12	169854314
+SCYL3	CRISPRko	TTATTGTGTGTTAGGTGTCCC	TGGAA	0.3233	29	175	+	303	4	169876090
+SCYL3	CRISPRko	CAGGTAATATAAGAAAAGCAG	CAGAA	0.3879	82	176	+	252	12	169854377
+SCYL3	CRISPRko	AAGCAGCAGAAGGCTTAATTT	CTGGG	0.4282	125	177	+	209	12	169854392
+SCYL3	CRISPRko	TTATGGGAAATTTAATAGGCT	GAGAA	0.3623	60	178	+	275	12	169854947
+SCYL3	CRISPRko	GCTTGGACCAGAGGTGGTTGT	GGGAG	0.5849	260	179	-	75	11	169859118
+SCYL3	CRISPRko	CCCGTGACTGGAACAGGGCTG	GTGAG	0.4599	161	180	+	174	10	169862759
+SCYL3	CRISPRko	CCCAGTGCACTACCTTGGATG	TGGAA	0.3881	85	181	-	251	12	169854749
+SCYL3	CRISPRko	CTTCAAACAACTGGAGAAGCA	CGGGG	0.5607	247	182	+	89	10	169862729
+SCYL3	CRISPRko	TCATCTTACCATCTCTTCAGG	AGGGA	0.6736	284	183	+	52	5	169873705
+SCYL3	CRISPRko	AGAAATGATTTTCTGGAAGTT	GTGAA	0.5627	250	184	-	87	8	169866958
+SCYL3	CRISPRko	AAAGGCCCCTTAAGGTTCCAT	CAGAA	0.4488	150	185	-	188	12	169854491
+SCYL3	CRISPRko	TACTCTGTCATGAAGGAAGAT	AAGAG	0.5512	243	186	+	96	3	169878649
+SCYL3	CRISPRko	TTGGATGTGGAAGAGTCATCT	TGGGA	0.6336	279	187	-	61	12	169854735
+SCYL3	CRISPRko	TTGAAGAGTGAAGAGGAGAAA	ACGGA	0.7024	303	188	-	37	8	169866913
+SCYL3	CRISPRko	AGTGGCCTGACTGGAGTGAAC	CTGAG	0.4662	174	189	-	167	12	169854839
+SCYL3	CRISPRko	AGGTTACTTACTCTGTCATGA	AGGAA	0.3323	43	190	+	300	3	169878641
+SCYL3	CRISPRko	ACCAAGTTCTGATGGAACCTT	AAGGG	0.4663	179	191	+	165	12	169854495
+SCYL3	CRISPRko	TCACAACTCTCCCAGAGTGTC	ATGGA	0.3111	30	192	-	314	6	169870330
+SCYL3	CRISPRko	ATACAGGTTACTTACTCTGTC	ATGAA	0.3937	101	193	+	245	3	169878637
+SCYL3	CRISPRko	GACACTGGAAGCTAGGAGGAA	TGGAA	0.5881	274	194	-	74	4	169876019
+SCYL3	CRISPRko	GACAGAGTCAGCTGCTTGTCA	GAGGA	0.5543	257	195	-	93	9	169864481
+SCYL3	CRISPRko	GGTTCCATCAGAACTTGGTTT	AGGAG	0.5712	271	196	-	82	12	169854478
+SCYL3	CRISPRko	CCGGGCATGTCCATGACACTC	TGGGA	0.3867	97	197	+	256	6	169870331
+SCYL3	CRISPRko	TTTCAAGAAATTCACAACTTC	CAGAA	0.3593	76	198	+	279	8	169866958
+SCYL3	CRISPRko	CTGGGGAGACATCATCCTTTT	TTGGG	0.4993	227	199	+	130	12	169854332
+SCYL3	CRISPRko	CGGATGGCATTCATCTTGTCA	CTGAG	0.3874	103	200	-	254	3	169878744
+SCYL3	CRISPRko	TTTCTGCTGCAGCAAATTTTG	AGGAA	0.3913	110	201	+	248	12	169854296
+SCYL3	CRISPRko	TTTTACTGGCTTCTTTTTTAC	TTGAA	0.5548	266	202	+	92	12	169854450
+SCYL3	CRISPRko	TGCTGGTCTCTCTGCTTGGAC	CAGAG	0.4157	138	203	-	220	11	169859131
+SCYL3	CRISPRko	GCTGAAGGCTGGGAAGAAGAA	GGGGA	0.6756	315	204	-	49	13	169853749
+SCYL3	CRISPRko	TCTTATTGACTGAATACTCCT	CAGAA	0.3857	107	205	+	258	5	169873744
+SCYL3	CRISPRko	ATTTATGGGAAATTTAATAGG	CTGAG	0.3425	74	206	+	294	12	169854945
+SCYL3	CRISPRko	GGCTTCTTTTTTACTTGAATG	GTGAA	0.5842	294	207	+	76	12	169854457
+SCYL3	CRISPRko	TCCAGTTGTTTGAAGTTCATG	AAGAG	0.4964	236	208	-	135	10	169862708
+SCYL3	CRISPRko	TTAACATTGAAGAGTGAAGAG	GAGAA	0.5664	286	209	-	86	8	169866919
+SCYL3	CRISPRko	AGTTTAACATTGAAGAGTGAA	GAGGA	0.5096	249	210	-	123	8	169866922
+SCYL3	CRISPRko	CTGCTACAAAACCTGTTACCT	CAGGG	0.3699	104	211	-	269	12	169854656
+SCYL3	CRISPRko	GCTGAGAAAATGGATCGCCTG	TAGGG	0.4727	216	212	+	159	12	169854965
+SCYL3	CRISPRko	ACTTTCTTCAGCTGCTCCTGA	GTGAA	0.5752	298	213	+	79	10	169862644
+SCYL3	CRISPRko	TTCGGGTAGATCATGCGCAGG	GAGAA	0.4674	215	214	-	162	10	169862789
+SCYL3	CRISPRko	AGAGGTGGTTGTGGGAGGAGA	ACGAA	0.5792	301	215	-	77	11	169859109
+SCYL3	CRISPRko	TTTCCTCAAAATTTGCTGCAG	CAGAA	0.3686	109	216	-	270	12	169854287
+SCYL3	CRISPRko	CACCATCCGCACATGCTCTTC	ATGAA	0.4264	170	217	+	210	10	169862703
+SCYL3	CRISPRko	CTGAAGGCTGGGAAGAAGAAG	GGGAG	0.6188	317	218	-	64	13	169853748
+SCYL3	CRISPRko	TCCCAGTGCACTACCTTGGAT	GTGGA	0.3413	87	219	-	295	12	169854750
+SCYL3	CRISPRko	CTGAGGAGTGGCCTGACTGGA	GTGAA	0.5083	258	220	-	124	12	169854845
+SCYL3	CRISPRko	CACAACCACCTCTGGTCCAAG	CAGAG	0.4673	219	221	+	163	11	169859132
+SCYL3	CRISPRko	CTTCAGTGAGTGAAAGCAAAG	CAGGA	0.4583	209	222	+	175	12	169854626
+SCYL3	CRISPRko	GGCTGGGAAGAAGAAGGGGAG	CTGAA	0.6046	318	223	-	67	13	169853743
+SCYL3	CRISPRko	TGTCCTCAGTTCAGGTAATAT	AAGAA	0.4167	168	224	+	218	12	169854366
+SCYL3	CRISPRko	GGACACTGGAAGCTAGGAGGA	ATGGA	0.4556	210	225	-	180	4	169876020
+SCYL3	CRISPRko	AATTTGCTGCAGCAGAAATTA	CTGAG	0.5688	306	226	-	85	12	169854278
+SCYL3	CRISPRko	GTTTATCATCTGTGTTTGTGA	GTGAA	0.4392	202	227	-	198	4	169876049
+SCYL3	CRISPRko	ACTGGAGTGAACCTGAGGAGC	CTGAA	0.465	230	228	-	171	12	169854830
+SCYL3	CRISPRko	TCTCAGATGTAAAAAATACTT	CGGAG	0.4457	212	229	-	189	12	169854905
+SCYL3	CRISPRko	ATTTCTGCTGCAGCAAATTTT	GAGGA	0.3727	136	230	+	267	12	169854295
+SCYL3	CRISPRko	TTTTGAGGAAAACTGCATCAC	TGGGG	0.3451	113	231	+	291	12	169854312
+SCYL3	CRISPRko	AGTGCAAGGTCTGTTGAAAGC	TGGAG	0.4118	180	232	+	224	7	169869016
+SCYL3	CRISPRko	CCATCAAGTTCTAAAAAGTCT	GAGGA	0.4333	204	233	-	203	12	169854864
+SCYL3	CRISPRko	GACTCTGTCCAGCAGAAATCT	GAGGA	0.4574	231	234	+	176	9	169864508
+SCYL3	CRISPRko	GTTGCAGGGAGAGGCTGAAGG	CTGGG	0.5531	316	235	-	94	13	169853762
+SCYL3	CRISPRko	AAGAAGAAGGGGAGCTGAACT	GGGAA	0.5424	312	236	-	99	13	169853736
+SCYL3	CRISPRko	TATCTCATGACTTCTTCAGGT	GAGGG	0.4	176	237	-	237	7	169868929
+SCYL3	CRISPRko	TAATTTCTGCTGCAGCAAATT	TTGAG	0.412	192	238	+	223	12	169854293
+SCYL3	CRISPRko	TCCCCGTGCTTCTCCAGTTGT	TTGAA	0.3306	114	239	-	301	10	169862720
+SCYL3	CRISPRko	CAAAGTTGAGTGCAAGGTCTG	TTGAA	0.3442	123	240	+	292	7	169869008
+SCYL3	CRISPRko	TACAGCCCCTGGAAGTGGCTT	TGGAA	0.4546	233	241	-	182	3	169878714
+SCYL3	CRISPRko	TCAGACTTTTTAGAACTTGAT	GGGAA	0.3657	144	242	+	274	12	169854877
+SCYL3	CRISPRko	GGAAGAAGAAGGGGAGCTGAA	CTGGG	0.5258	311	243	-	108	13	169853738
+SCYL3	CRISPRko	CTGCTGCTTTTCTTATATTAC	CTGAA	0.3703	152	244	-	268	12	169854365
+SCYL3	CRISPRko	TGTCTTCTGCAGAGGTCTGTG	CTGGG	0.4114	195	245	-	225	3	169878684
+SCYL3	CRISPRko	ACAGGGCTGGTGAGAGCAAGC	AAGGA	0.4769	267	246	+	154	10	169862771
+SCYL3	CRISPRko	TCACTGAGCGAGTACAGCCCC	TGGAA	0.4651	253	247	-	170	3	169878726
+SCYL3	CRISPRko	AAAAGATTAGCCTTGTACAAA	GGGGG	0.4821	276	248	-	149	12	169854560
+SCYL3	CRISPRko	TTCTCCAGTTGTTTGAAGTTC	ATGAA	0.3801	163	249	-	263	10	169862711
+SCYL3	CRISPRko	TTTCTCTCTTATACACAAAAA	CTGAA	0.3607	153	250	+	276	2	169888723
+SCYL3	CRISPRko	GGTCAGTATTTTTAGTAAAAC	TTGGG	0.3666	157	251	+	273	11	169859071
+SCYL3	CRISPRko	TGCTTAATCAGTTGGTGTTTG	CAGAG	0.4504	244	252	-	187	9	169864426
+SCYL3	CRISPRko	ATGGGAAGTTTTCACTGTCCT	CCGAA	0.4007	197	253	+	236	12	169854896
+SCYL3	CRISPRko	TCACGCAGGCCCAGCAAAACC	TAGGA	0.418	217	254	+	217	11	169859211
+SCYL3	CRISPRko	CTCTTCATGAACTTCAAACAA	CTGGA	0.4019	199	255	+	235	10	169862718
+SCYL3	CRISPRko	TTCATGAACTTCAAACAACTG	GAGAA	0.5032	307	256	+	127	10	169862721
+SCYL3	CRISPRko	ACTTCAAACAACTGGAGAAGC	ACGGG	0.4778	282	257	+	152	10	169862728
+SCYL3	CRISPRko	AGGTTCCATCAGAACTTGGTT	TAGGA	0.2677	116	258	-	320	12	169854479
+SCYL3	CRISPRko	CCTTTTCATTTGGAACATTGG	TGGAA	0.4234	225	259	-	211	6	169870291
+SCYL3	CRISPRko	GACCAGAGGTGGTTGTGGGAG	GAGAA	0.493	299	260	-	138	11	169859113
+SCYL3	CRISPRko	CTTTGGAAACATTGTCTTCTG	CAGAG	0.4858	293	261	-	146	3	169878696
+SCYL3	CRISPRko	CTGACTCTGTCCAGCAGAAAT	CTGAG	0.4214	228	262	+	214	9	169864506
+SCYL3	CRISPRko	CCTCAGACTTTTTAGAACTTG	ATGGG	0.4295	238	263	+	207	12	169854875
+SCYL3	CRISPRko	AATTTCTTGAAAAGTTTAACA	TTGAA	0.3578	165	264	-	280	8	169866934
+SCYL3	CRISPRko	GAGCACTTCACTCAGGAGCAG	CTGAA	0.4779	296	265	-	151	10	169862640
+SCYL3	CRISPRko	CACTTCACTCAGGAGCAGCTG	AAGAA	0.4771	297	266	-	153	10	169862637
+SCYL3	CRISPRko	AGGGAGAGGCTGAAGGCTGGG	AAGAA	0.4968	323	267	-	133	13	169853757
+SCYL3	CRISPRko	TTCCTGCTTTGCTTTCACTCA	CTGAA	0.4024	223	268	-	234	12	169854617
+SCYL3	CRISPRko	TCACTGCATAAGGGATGGGAT	CAGAG	0.405	229	269	-	230	2	169888837
+SCYL3	CRISPRko	CTCACTGAAGAGTCTATGCCT	TGGAA	0.3546	178	270	-	282	12	169854600
+SCYL3	CRISPRko	CTCTTCAATGTTAAACTTTTC	AAGAA	0.3381	164	271	+	296	8	169866941
+SCYL3	CRISPRko	GGCTGAAGGCTGGGAAGAAGA	AGGGG	0.4905	321	272	-	141	13	169853750
+SCYL3	CRISPRko	CTTTTTGTCCTCAGATTTCTG	CTGGA	0.3185	154	273	-	308	9	169864505
+SCYL3	CRISPRko	CTAAAAATACTGACCTTTCTC	TAGAA	0.2493	142	274	-	321	11	169859050
+SCYL3	CRISPRko	CTCAGACTTTTTAGAACTTGA	TGGGA	0.3162	156	275	+	310	12	169854876
+SCYL3	CRISPRko	CAAGCAGCTGACTCTGTCCAG	CAGAA	0.4571	290	276	+	177	9	169864499
+SCYL3	CRISPRko	GCCTTTTCATTTGGAACATTG	GTGGA	0.3782	205	277	-	264	6	169870292
+SCYL3	CRISPRko	TTATATTACCTGAACTGAGGA	CAGAA	0.429	263	278	-	208	12	169854353
+SCYL3	CRISPRko	CTCTCAGATGTAAAAAATACT	TCGGA	0.3158	162	279	-	311	12	169854906
+SCYL3	CRISPRko	TTACATCTGAGAGTCCATTTA	TGGGA	0.3239	175	280	+	302	12	169854929
+SCYL3	CRISPRko	GAGAGGCTGAAGGCTGGGAAG	AAGAA	0.4751	324	281	-	156	13	169853754
+SCYL3	CRISPRko	CACACCAGATGCCCTCACCTG	AAGAA	0.4451	289	282	+	191	7	169868928
+SCYL3	CRISPRko	TTGGACCAGAGGTGGTTGTGG	GAGGA	0.4453	291	283	-	190	11	169859116
+SCYL3	CRISPRko	GGCTTACCTCTGGTGTGGCCT	GAGAA	0.3954	242	284	+	243	4	169875989
+SCYL3	CRISPRko	TACATCTGAGAGTCCATTTAT	GGGAA	0.3498	200	285	+	287	12	169854930
+SCYL3	CRISPRko	TGCTTGGACCAGAGGTGGTTG	TGGGA	0.3834	226	286	-	261	11	169859119
+SCYL3	CRISPRko	ACTCTGGGAGAGTTGTGAATT	CTGGA	0.3352	189	287	+	299	6	169870348
+SCYL3	CRISPRko	TGGACAGAGTCAGCTGCTTGT	CAGAG	0.319	182	288	-	307	9	169864483
+SCYL3	CRISPRko	CAGGTTACTTACTCTGTCATG	AAGGA	0.2847	171	289	+	319	3	169878640
+SCYL3	CRISPRko	TTTTCTTATATTACCTGAACT	GAGGA	0.3137	183	290	-	312	12	169854358
+SCYL3	CRISPRko	TTTGAGGAAAACTGCATCACT	GGGGA	0.4164	277	291	+	219	12	169854313
+SCYL3	CRISPRko	TTTCTGGAAGTTGTGAATTTC	TTGAA	0.3178	191	292	-	309	8	169866949
+SCYL3	CRISPRko	GAAGAAGAAGGGGAGCTGAAC	TGGGA	0.445	310	293	-	192	13	169853737
+SCYL3	CRISPRko	TGGGGAGACATCATCCTTTTT	TGGGA	0.2856	196	294	+	318	12	169854333
+SCYL3	CRISPRko	AGGCTGAAGGCTGGGAAGAAG	AAGGG	0.4443	322	295	-	193	13	169853751
+SCYL3	CRISPRko	TAGCAGTGCTGGTCTCTCTGC	TTGGA	0.4094	287	296	-	228	11	169859137
+SCYL3	CRISPRko	TGCAGGGAGAGGCTGAAGGCT	GGGAA	0.4407	319	297	-	197	13	169853760
+SCYL3	CRISPRko	CTGGCTTACCTCTGGTGTGGC	CTGAG	0.3773	252	298	+	266	4	169875987
+SCYL3	CRISPRko	AGATCCCAGCACAGACCTCTG	CAGAA	0.4184	305	299	+	216	3	169878690
+SCYL3	CRISPRko	TAAGATTTTTATCTTGTACTG	TGGAA	0.3369	224	300	-	297	3	169878771
+SCYL3	CRISPRko	CTAAGATTTTTATCTTGTACT	GTGGA	0.3004	208	301	-	315	3	169878772
+SCYL3	CRISPRko	CTTCAGTTTTTGTGTATAAGA	GAGAA	0.3842	264	302	-	259	2	169888714
+SCYL3	CRISPRko	TGGACCAGAGGTGGTTGTGGG	AGGAG	0.3871	273	303	-	255	11	169859115
+SCYL3	CRISPRko	TTTGCTTCAGTTTTTGTGTAT	AAGAG	0.351	248	304	-	284	2	169888718
+SCYL3	CRISPRko	TGAAAAAGCATTCACCTTTTT	TGGGG	0.3469	245	305	+	290	9	169864372
+SCYL3	CRISPRko	ATCATCTTACCATCTCTTCAG	GAGGG	0.3861	278	306	+	257	5	169873704
+SCYL3	CRISPRko	TCCCATCAAGTTCTAAAAAGT	CTGAG	0.3668	270	307	-	272	12	169854866
+SCYL3	CRISPRko	ATATCATCTTACCATCTCTTC	AGGAG	0.35	262	308	+	285	5	169873702
+SCYL3	CRISPRko	TATATCATCTTACCATCTCTT	CAGGA	0.3119	239	309	+	313	5	169873701
+SCYL3	CRISPRko	TTTACATCTGAGAGTCCATTT	ATGGG	0.3817	295	310	+	262	12	169854928
+SCYL3	CRISPRko	TTGAAAAAGCATTCACCTTTT	TTGGG	0.3937	313	311	+	244	9	169864371
+SCYL3	CRISPRko	CTTTTTTGGGGCCAAGCAGAT	AAGGA	0.3494	269	312	+	289	9	169864387
+SCYL3	CRISPRko	TTGCAGGGAGAGGCTGAAGGC	TGGGA	0.3985	320	313	-	240	13	169853761
+SCYL3	CRISPRko	TTATTTTGTTGCAGGGAGAGG	CTGAA	0.3919	314	314	-	247	13	169853769
+SCYL3	CRISPRko	TTATTTCTCTGTCTAGTTTCT	GAGGA	0.3773	300	315	-	265	5	169873751
+SCYL3	CRISPRko	ATTTATTTCTCTGTCTAGTTT	CTGAG	0.3601	288	316	-	277	5	169873753
+SCYL3	CRISPRko	CTTTTAATAATTCTTACCTTC	TAGAG	0.3499	281	317	+	286	11	169859042
+SCYL3	CRISPRko	CGGTTTGTATTTTTAAAGCAT	TTGAA	0.2211	251	318	-	323	3	169878820
+SCYL3	CRISPRko	CATCTGTGTTTGTGAGTGAAG	ATGGA	0.3209	272	319	-	306	4	169876043
+SCYL3	CRISPRko	GTACAGCCCCTGGAAGTGGCT	TTGGA	0.3495	292	320	-	288	3	169878715
+SCYL3	CRISPRko	TTTAATAATTCTTACCTTCTA	GAGAA	0.2344	268	321	+	322	11	169859044
+SCYL3	CRISPRko	GTCAGTATTTTTAGTAAAACT	TGGGG	0.3002	304	322	+	316	11	169859072
+SCYL3	CRISPRko	TGGCATTTAGAAATGATTTTC	TGGAA	0.2995	308	323	-	317	8	169866966
+SCYL3	CRISPRko	TTGGCATTTAGAAATGATTTT	CTGGA	0.2128	309	324	-	324	8	169866967
+FIRRM	CRISPRko	TTACCTTAGCGTAGTGCAAAA	GGGAG	0.8531	15	1	-	6	10	169821746
+FIRRM	CRISPRko	ACAGTTGGTGCAAAGTACAGC	AAGAA	0.8224	20	2	-	12	11	169823439
+FIRRM	CRISPRko	ATGTGAGCAGCTGACTGATAA	GTGGA	0.9004	32	3	-	2	12	169827143
+FIRRM	CRISPRko	TTCAGCATATCTGTGCCACAC	AGGAA	0.7851	26	4	+	16	6	169803224
+FIRRM	CRISPRko	CTGAAGTATCAGTTTAGGATC	TAGAG	0.7374	30	5	-	24	20	169843752
+FIRRM	CRISPRko	TTTGCCTTACTGCTAGCTGAC	AGGAG	0.6806	17	6	+	42	22	169849548
+FIRRM	CRISPRko	TTCAACAGTATTGATAGGTTG	ATGAG	0.7848	48	7	-	17	17	169832455
+FIRRM	CRISPRko	CCTGTTCATTTACAGGGATTA	AAGAG	0.8194	52	8	+	13	14	169829342
+FIRRM	CRISPRko	CTGAAACTAGGTGTAAACACC	CAGAA	0.7063	35	9	-	30	7	169804100
+FIRRM	CRISPRko	TTGGAAATCCAGACCACTCTA	AGGAA	0.6798	28	10	+	43	5	169802708
+FIRRM	CRISPRko	TTGGCAAAAAAACGACACAAT	TTGAG	0.8064	58	11	-	14	10	169821720
+FIRRM	CRISPRko	CAAACTGCAATTATCGAAGTT	GTGAG	0.6171	12	12	+	63	19	169842501
+FIRRM	CRISPRko	CTAAGAGCTGCACCATTGTCT	GTGAA	0.716	46	13	-	29	6	169803170
+FIRRM	CRISPRko	ACCATGCATCCATAGCTAACA	AAGAG	0.5809	4	14	-	75	15	169830311
+FIRRM	CRISPRko	AATTATTCGATGACATGATGT	ATGAA	0.6962	44	15	+	35	5	169802646
+FIRRM	CRISPRko	GCAAATATGTTGTACCGAGCA	AGGAA	0.5805	5	16	-	76	15	169830339
+FIRRM	CRISPRko	CCAGGATTTCTAAAGCACACC	AAGAG	0.5998	27	17	+	67	12	169827095
+FIRRM	CRISPRko	CCTTGTAATTCTACGCACCAG	ATGAG	0.5566	9	18	-	88	16	169830748
+FIRRM	CRISPRko	ATATTTTGAATATGGTAATAG	GTGAG	0.7894	86	19	+	15	7	169804238
+FIRRM	CRISPRko	CTCATCAACCTATCAATACTG	TTGAA	0.5607	19	20	+	84	17	169832465
+FIRRM	CRISPRko	CCTGGTAGCATAAAGGCTTGG	AGGAA	0.5979	36	21	-	68	12	169827064
+FIRRM	CRISPRko	TTTTATAATTACCTCAGCGAA	CTGAG	0.7463	93	22	-	22	22	169849599
+FIRRM	CRISPRko	AGAGCAGATATCCAATAAAGA	ATGAA	0.6912	79	23	-	37	8	169806015
+FIRRM	CRISPRko	TGCTTACCAGTTCAGCAAACA	GTGAG	0.5756	39	24	-	79	14	169829439
+FIRRM	CRISPRko	TCAAAACTGTAGAAAACGGCT	TTGAG	0.7656	103	25	-	19	14	169829281
+FIRRM	CRISPRko	CATTGCCAAACGAACATAGTC	AGGAA	0.5156	2	26	-	121	21	169847735
+FIRRM	CRISPRko	ATGATCACCTCTTTGTTAGCT	ATGGA	0.5617	45	27	+	82	15	169830313
+FIRRM	CRISPRko	GACCTGGCTGTCTGTGCACCA	CAGGG	0.8347	119	28	-	9	13	169827806
+FIRRM	CRISPRko	GTATGAATTAACCAGTCAAGC	CAGAG	0.5221	14	29	+	115	5	169802665
+FIRRM	CRISPRko	GATATGCTGAACACATCCTGT	GAGAG	0.5554	40	30	-	91	6	169803199
+FIRRM	CRISPRko	ATGAATTAACCAGTCAAGCCA	GAGGA	0.5612	49	31	+	83	5	169802667
+FIRRM	CRISPRko	TTCAGCCTTTTACTTCCACTG	TTGGG	0.5973	67	32	+	69	20	169843722
+FIRRM	CRISPRko	GGTCTGCAAAGTTACTGCCTA	CAGAG	0.525	25	33	-	113	21	169847690
+FIRRM	CRISPRko	AGAATAGTGAATCTGTGTATT	CTGGG	0.6524	88	34	+	53	7	169804091
+FIRRM	CRISPRko	GTTAATTCATACATCATGTCA	TCGAA	0.5817	68	35	-	74	5	169802641
+FIRRM	CRISPRko	CATAAGGTTGATCTGCTACCT	AAGAA	0.5046	16	36	-	128	20	169843675
+FIRRM	CRISPRko	CTGTATCCAAAGCTTCACCAG	CAGAA	0.6862	105	37	-	40	19	169842460
+FIRRM	CRISPRko	TAACAGAGCAGATATCCAATA	AAGAA	0.6171	82	38	-	64	8	169806019
+FIRRM	CRISPRko	TCCTCCAAGCCTTTATGCTAC	CAGGA	0.6757	101	39	+	46	12	169827075
+FIRRM	CRISPRko	CAGGAAGCTCTAATTTAAGTA	GCGAG	0.5264	38	40	-	111	21	169847715
+FIRRM	CRISPRko	AAGAGAATAGGCCTCCTTGAA	AAGAG	0.5449	59	41	-	96	7	169804138
+FIRRM	CRISPRko	AGCACATTTGTGCATTGTAAG	GTGAG	0.7443	132	42	+	23	6	169803307
+FIRRM	CRISPRko	GACTATGTTCGTTTGGCAATG	TTGGA	0.4991	23	43	+	134	21	169847750
+FIRRM	CRISPRko	CCCTGTAAATGAACAGGTAGA	GAGAG	0.8979	154	44	-	3	14	169829323
+FIRRM	CRISPRko	GGAGTCGTACTTTGGGAGAAC	TAGAA	0.6603	108	45	+	52	18	169837099
+FIRRM	CRISPRko	ACAGTAGGCACTGCAGAATGC	AGGAA	0.5295	56	46	+	104	18	169837062
+FIRRM	CRISPRko	CTTATCCATGACAACAACCAG	AAGAA	0.4879	18	47	-	143	13	169827752
+FIRRM	CRISPRko	GATTAAAGAGTAAGGGGAAAG	CTGAG	0.5878	90	48	+	73	14	169829358
+FIRRM	CRISPRko	TCTCTCTACCTGTTCATTTAC	AGGGA	0.6457	110	49	+	55	14	169829334
+FIRRM	CRISPRko	TATCAGTTTAGGATCTAGAGT	TTGAA	0.6695	118	50	-	49	20	169843746
+FIRRM	CRISPRko	TCCAGATAAAGTCATGCCCTG	GAGAA	0.7301	141	51	+	26	17	169832430
+FIRRM	CRISPRko	TGGAGGCGTTTACTCAGTTCG	CTGAG	0.4694	6	52	+	163	22	169849597
+FIRRM	CRISPRko	TATTTATCCGTAATCAGAATA	GTGAA	0.6445	115	53	+	56	7	169804076
+FIRRM	CRISPRko	TGTATCTTCAGATACACAGGT	AAGAA	0.5382	72	54	+	101	11	169823472
+FIRRM	CRISPRko	ATCCCTGTAAATGAACAGGTA	GAGAG	0.5092	60	55	-	122	14	169829325
+FIRRM	CRISPRko	TGCAGACCTCGCTACTTAAAT	TAGAG	0.4557	7	56	+	176	21	169847718
+FIRRM	CRISPRko	TTACTGTCCAAAACCACCTGC	ATGAA	0.4801	34	57	-	153	12	169827177
+FIRRM	CRISPRko	CCTAAGCTCAGGAGGTAACGC	CTGGA	0.4518	10	58	-	178	18	169837002
+FIRRM	CRISPRko	CTTACCTTAGCGTAGTGCAAA	AGGGA	0.4494	3	59	-	186	10	169821747
+FIRRM	CRISPRko	CAGATATGCTGAACACATCCT	GTGAG	0.5564	100	60	-	89	6	169803201
+FIRRM	CRISPRko	GGCTGCTAGAACAACATACCT	TGGAG	0.5936	122	61	+	70	22	169849576
+FIRRM	CRISPRko	TTTTAATTATATGAAGGACTG	AGGAG	0.5173	73	62	-	120	6	169803272
+FIRRM	CRISPRko	GTTTGCCTTACTGCTAGCTGA	CAGGA	0.4605	24	63	+	171	22	169849547
+FIRRM	CRISPRko	GACAGTTTGTTCCCTAAGCTC	AGGAG	0.4828	53	64	-	150	18	169837014
+FIRRM	CRISPRko	TCTGCTCAGCTAACTGTAAAC	AAGAA	0.5275	96	65	-	109	9	169807892
+FIRRM	CRISPRko	AAACATACAGGACAGGTTGGG	CAGAA	0.6081	142	66	-	66	22	169849515
+FIRRM	CRISPRko	ATGATATCCTTCCTTGCCTGA	TGGGG	0.6824	167	67	-	41	17	169832506
+FIRRM	CRISPRko	GAACAGGTAGAGAGAGTTCAC	CAGAA	0.495	74	68	-	136	14	169829313
+FIRRM	CRISPRko	TCAGCAAACAGTGAGGGATGA	AAGGA	0.5071	85	69	-	126	14	169829428
+FIRRM	CRISPRko	TTAATTATATGAAGGACTGAG	GAGGG	0.4889	70	70	-	141	6	169803270
+FIRRM	CRISPRko	CAGTTCAGCAAACAGTGAGGG	ATGAA	0.5012	80	71	-	132	14	169829432
+FIRRM	CRISPRko	AAGATATAATTACTAGCTTGT	GTGAA	0.4831	63	72	+	149	9	169807856
+FIRRM	CRISPRko	TTTGCTACCTGGTTGTCGTTT	CTGAG	0.437	11	73	-	203	13	169827831
+FIRRM	CRISPRko	TGCAAAGTACAGCAAGAATCA	GAGAG	0.634	157	74	-	58	11	169823431
+FIRRM	CRISPRko	CAGCAAACAGTGAGGGATGAA	AGGAA	0.4905	78	75	-	139	14	169829427
+FIRRM	CRISPRko	TATCATCATTGTCATCTACTA	AAGGG	0.752	200	76	-	20	7	169804205
+FIRRM	CRISPRko	GCTTTGAGTAGAGATATCCTG	CAGGG	0.5293	117	77	-	105	14	169829263
+FIRRM	CRISPRko	GCATGAAATGCATGGTCCATA	CTGGA	0.451	42	78	-	180	8	169806047
+FIRRM	CRISPRko	ATGGCACCACCCCATCAGGCA	AGGAA	0.4581	50	79	+	173	17	169832507
+FIRRM	CRISPRko	CCCAACAGTGGAAGTAAAAGG	CTGAA	0.7254	197	80	-	28	20	169843712
+FIRRM	CRISPRko	ATTACATGTAAGACCTGCCAT	GTGAA	0.5034	98	81	+	129	13	169827717
+FIRRM	CRISPRko	CATGAAATGCATGGTCCATAC	TGGAA	0.4952	94	82	-	135	8	169806046
+FIRRM	CRISPRko	AATTATATGAAGGACTGAGGA	GGGGA	0.5885	158	83	-	72	6	169803268
+FIRRM	CRISPRko	CCATGCATTTCATGCCAATAC	TTGGA	0.4294	21	84	+	211	8	169806068
+FIRRM	CRISPRko	TCCTAAACTGATACTTCAGGT	AAGAA	0.6166	170	85	+	65	20	169843769
+FIRRM	CRISPRko	GCTCAGGAGGTAACGCCTGGA	AGGAA	0.5248	123	86	-	114	18	169836997
+FIRRM	CRISPRko	TCAGCCTTTTACTTCCACTGT	TGGGA	0.5267	128	87	+	110	20	169843723
+FIRRM	CRISPRko	AGGTCACCACAGTAGGCACTG	CAGAA	0.7302	213	88	+	25	18	169837054
+FIRRM	CRISPRko	TTTCCTTCCAGGCGTTACCTC	CTGAG	0.487	95	89	+	144	18	169837006
+FIRRM	CRISPRko	AAGGACTGAGGAGGGGAGACT	CTGAA	0.6774	195	90	-	44	6	169803259
+FIRRM	CRISPRko	ACCTGCATGAAAGGCTGAAAT	GTGAG	0.5694	161	91	-	80	12	169827162
+FIRRM	CRISPRko	ACTGTAGAAAACGGCTTTGAG	TAGAG	0.5178	124	92	-	119	14	169829276
+FIRRM	CRISPRko	TTCTTACCTGAAGTATCAGTT	TAGGA	0.5764	166	93	-	78	20	169843759
+FIRRM	CRISPRko	TAATCCCTGTAAATGAACAGG	TAGAG	0.4409	51	94	-	194	14	169829327
+FIRRM	CRISPRko	GAATGCAGGAAATGGCTGAGC	AGGAG	0.9736	245	95	+	1	18	169837077
+FIRRM	CRISPRko	AACTGTAAACAAGAATGGAAG	GAGAA	0.7281	220	96	-	27	9	169807881
+FIRRM	CRISPRko	CTAAGCTCAGGAGGTAACGCC	TGGAA	0.4127	22	97	-	228	18	169837001
+FIRRM	CRISPRko	TTTGATTAGGATGCTGCTCTG	CTGAA	0.5936	181	98	+	71	15	169830268
+FIRRM	CRISPRko	CCTCATATGAACCACCTGACA	TTGGA	0.4199	31	99	+	221	4	169800933
+FIRRM	CRISPRko	CAAAGTACAGCAAGAATCAGA	GAGGA	0.6757	210	100	-	45	11	169823429
+FIRRM	CRISPRko	AGCAAATATGTTGTACCGAGC	AAGGA	0.3837	1	101	-	255	15	169830340
+FIRRM	CRISPRko	CATGGTCCATACTGGAAATAA	CAGAG	0.5085	134	102	-	124	8	169806037
+FIRRM	CRISPRko	TGCAGCTCTTAGGAGCTCTCA	CAGGA	0.6967	225	103	+	34	6	169803194
+FIRRM	CRISPRko	TAAACAAGAATGGAAGGAGAA	AAGAA	0.7806	242	104	-	18	9	169807876
+FIRRM	CRISPRko	ACAGCAAGAATCAGAGAGGAA	AGGAA	0.6901	222	105	-	39	11	169823423
+FIRRM	CRISPRko	CCTTACCTTAGCGTAGTGCAA	AAGGG	0.3855	8	106	-	253	10	169821748
+FIRRM	CRISPRko	AGCTCAGGAGGTAACGCCTGG	AAGGA	0.4713	102	107	-	160	18	169836998
+FIRRM	CRISPRko	TATATTGCAGATATGGGACTG	CTGAA	0.4486	75	108	+	188	16	169830704
+FIRRM	CRISPRko	CTTATGCAAAAAGAGCTCGTC	AGGAG	0.8646	260	109	+	4	25	169852809
+FIRRM	CRISPRko	ACATTGCCAAACGAACATAGT	CAGGA	0.3866	13	110	-	252	21	169847736
+FIRRM	CRISPRko	GCAACTGAGTCACTACTCCAA	AAGGG	0.8607	262	111	+	5	25	169852892
+FIRRM	CRISPRko	GGATTTCTAAAGCACACCAAG	AGGAA	0.5019	139	112	+	131	12	169827098
+FIRRM	CRISPRko	GCTAACTGTAAACAAGAATGG	AAGGA	0.5194	153	113	-	117	9	169807884
+FIRRM	CRISPRko	AGCACTAAGTACAGCATTCAG	CAGAG	0.4209	54	114	-	218	15	169830274
+FIRRM	CRISPRko	CAGCTTATCCATGACAACAAC	CAGAA	0.5277	164	115	-	108	13	169827755
+FIRRM	CRISPRko	TAAAATATTCCTTAGAGTGGT	CTGGA	0.4854	127	116	-	147	5	169802705
+FIRRM	CRISPRko	GCAAGAATCAGAGAGGAAAGG	AAGAA	0.6944	239	117	-	36	11	169823420
+FIRRM	CRISPRko	AAAGTACAGCAAGAATCAGAG	AGGAA	0.63	215	118	-	61	11	169823428
+FIRRM	CRISPRko	CAGTCCATAATAAAAAGCCAG	TTGAA	0.5578	190	119	+	86	9	169807825
+FIRRM	CRISPRko	TTTGGAAATCCAGACCACTCT	AAGGA	0.5432	178	120	+	99	5	169802707
+FIRRM	CRISPRko	ATTATACCTTTGGTAACACTT	GTGAA	0.3908	33	121	-	246	4	169800961
+FIRRM	CRISPRko	TACAGCAAGAATCAGAGAGGA	AAGGA	0.6439	223	122	-	57	11	169823424
+FIRRM	CRISPRko	TTGCAGTATGTAATTCTGCTG	GTGAA	0.4215	64	123	+	217	19	169842457
+FIRRM	CRISPRko	AAGTAAAAGGCTGAATGTCTG	TTGAA	0.4218	66	124	-	216	20	169843701
+FIRRM	CRISPRko	CTTACCAGTTCAGCAAACAGT	GAGGG	0.577	205	125	-	77	14	169829437
+FIRRM	CRISPRko	CAGGGTTTGCACTTCCTTAGG	CTGAG	0.518	165	126	-	118	13	169827785
+FIRRM	CRISPRko	TCTCCAGGGCATGACTTTATC	TGGAA	0.4111	55	127	-	230	17	169832419
+FIRRM	CRISPRko	AAGGCTTGGAGGAAACTTGCT	GGGAA	0.465	120	128	-	166	12	169827052
+FIRRM	CRISPRko	CTTACTCTTTAATCCCTGTAA	ATGAA	0.3819	29	129	-	258	14	169829336
+FIRRM	CRISPRko	TTACCAGTTCAGCAAACAGTG	AGGGA	0.67	240	130	-	48	14	169829436
+FIRRM	CRISPRko	CAAAACCACCTGCATGAAAGG	CTGAA	0.4868	143	131	-	145	12	169827169
+FIRRM	CRISPRko	AAAGGCTTGGAGGAAACTTGC	TGGGA	0.4562	113	132	-	175	12	169827053
+FIRRM	CRISPRko	TAATTATATGAAGGACTGAGG	AGGGG	0.4375	87	133	-	201	6	169803269
+FIRRM	CRISPRko	ATTATATGAAGGACTGAGGAG	GGGAG	0.5563	198	134	-	90	6	169803267
+FIRRM	CRISPRko	ACTTACTTGTAAAATATTCCT	TAGAG	0.6488	234	135	-	54	5	169802714
+FIRRM	CRISPRko	ATAAGCTGCCATCTCAGCCTA	AGGAA	0.4889	147	136	+	142	13	169827783
+FIRRM	CRISPRko	ATAGGCCTCCTTGAAAAGAGC	CTGGA	0.5577	203	137	-	87	7	169804132
+FIRRM	CRISPRko	GCCTAGTGATAAGTTTCACCC	TAGAG	0.8374	283	138	-	8	25	169852985
+FIRRM	CRISPRko	TAAAGGCTTGGAGGAAACTTG	CTGGG	0.5299	188	139	-	103	12	169827054
+FIRRM	CRISPRko	TCTTCTACAGTCACATTAGCA	AAGGG	0.831	284	140	-	10	24	169851897
+FIRRM	CRISPRko	CCAGAACACTGCTCAAAACTG	TAGAA	0.469	130	141	-	164	14	169829293
+FIRRM	CRISPRko	GAGGACTGTCAAGCCAAAATT	TGGAA	0.4505	112	142	+	183	5	169802688
+FIRRM	CRISPRko	TAAAACGTTACATACATACTC	TAGGG	0.7017	264	143	+	32	25	169852977
+FIRRM	CRISPRko	TATCGAAGTTGTGAGTCAGCT	TTGGG	0.4495	114	144	+	184	19	169842512
+FIRRM	CRISPRko	GGCTGAGCAGGAGTCGTACTT	TGGGA	0.4322	91	145	+	207	18	169837090
+FIRRM	CRISPRko	AGCTTAGGGAACAAACTGTCC	ATGAG	0.4411	106	146	+	193	18	169837030
+FIRRM	CRISPRko	GGAAATGGAGGCGCTCCAAGA	AAGGA	0.6905	263	147	+	38	25	169852942
+FIRRM	CRISPRko	CTCTCTCTACCTGTTCATTTA	CAGGG	0.4671	137	148	+	165	14	169829333
+FIRRM	CRISPRko	TTATCCAGAAATTTTCCCCAA	AAGAA	0.4747	148	149	+	157	18	169836952
+FIRRM	CRISPRko	CAGGTATAAAAAGTTTTCCTA	AAGAA	0.4168	81	150	-	225	21	169847775
+FIRRM	CRISPRko	CAGGCTGGAGCAGGACCCTTT	TGGAG	0.7467	286	151	-	21	25	169852897
+FIRRM	CRISPRko	TCAAAGCCGTTTTCTACAGTT	TTGAG	0.3898	62	152	+	247	14	169829292
+FIRRM	CRISPRko	CTAACTGTAAACAAGAATGGA	AGGAG	0.5528	216	153	-	93	9	169807883
+FIRRM	CRISPRko	AACAACCAGAAGAAGACATTG	TGGAA	0.6319	250	154	-	60	13	169827740
+FIRRM	CRISPRko	TAGCAGGGGCTTTGGAAGCAA	CTGAG	0.8518	305	155	+	7	25	169852875
+FIRRM	CRISPRko	TTTTCCAGATAAAGTCATGCC	CTGGA	0.3647	41	156	+	277	17	169832427
+FIRRM	CRISPRko	CAGGAAAACAAACTGCAATTA	TCGAA	0.463	155	157	+	168	19	169842493
+FIRRM	CRISPRko	TCCTGGTAGCATAAAGGCTTG	GAGGA	0.4811	172	158	-	151	12	169827065
+FIRRM	CRISPRko	AAAGAAAGTACCTTCTCCAGA	AAGGA	0.7029	294	159	-	31	23	169850339
+FIRRM	CRISPRko	TGTAATTCTGCTGGTGAAGCT	TTGGA	0.4892	185	160	+	140	19	169842465
+FIRRM	CRISPRko	AAATCCTGGTAGCATAAAGGC	TTGGA	0.3603	43	161	-	282	12	169827068
+FIRRM	CRISPRko	GGTGCACAGACAGCCAGGTCT	CAGAA	0.539	227	162	+	100	13	169827822
+FIRRM	CRISPRko	CTGCTGGTGAAGCTTTGGATA	CAGGA	0.3675	57	163	+	272	19	169842472
+FIRRM	CRISPRko	ATCAGTTTTATAATTACCTCA	GCGAA	0.5315	229	164	-	102	22	169849604
+FIRRM	CRISPRko	TCCATCATTTTGGAAAATATT	CAGAG	0.5278	224	165	+	107	6	169803250
+FIRRM	CRISPRko	GAAGCTGCCAAAGTGGAACGT	GTGAA	0.6688	282	166	+	50	24	169851856
+FIRRM	CRISPRko	ATTCTCTTCAAAAGCAGTTAA	TGGAA	0.4492	146	167	+	187	7	169804166
+FIRRM	CRISPRko	CAACAACCAGAAGAAGACATT	GTGGA	0.5438	237	168	-	98	13	169827741
+FIRRM	CRISPRko	CAGTTCCATTAACTGCTTTTG	AAGAG	0.4494	150	169	-	185	7	169804159
+FIRRM	CRISPRko	GCTGAGCAGGAGTCGTACTTT	GGGAG	0.4577	162	170	+	174	18	169837091
+FIRRM	CRISPRko	TTTTGAAGAGAATAGGCCTCC	TTGAA	0.4942	199	171	-	138	7	169804143
+FIRRM	CRISPRko	ACACATCCTGTGAGAGCTCCT	AAGAG	0.4762	182	172	-	155	6	169803189
+FIRRM	CRISPRko	TGCTTTTAATTATATGAAGGA	CTGAG	0.3887	89	173	-	249	6	169803275
+FIRRM	CRISPRko	GTCAGGAGTTCCCCTGGGAAG	AAGAG	0.8255	329	174	+	11	25	169852827
+FIRRM	CRISPRko	CTTTAGGAAAACTTTTTATAC	CTGAA	0.3751	76	175	+	264	21	169847787
+FIRRM	CRISPRko	TTCCAATGTCAGGTGGTTCAT	ATGAG	0.41	109	176	-	232	4	169800924
+FIRRM	CRISPRko	TTTAATAGGGAACAAATCATG	AAGAG	0.6207	280	177	+	62	23	169850275
+FIRRM	CRISPRko	TGAGCAGGAGTCGTACTTTGG	GAGAA	0.451	163	178	+	181	18	169837093
+FIRRM	CRISPRko	TTTCCAGATAAAGTCATGCCC	TGGAG	0.4083	111	179	+	233	17	169832428
+FIRRM	CRISPRko	AGGATTTCTAAAGCACACCAA	GAGGA	0.459	173	180	+	172	12	169827097
+FIRRM	CRISPRko	CCAGGCGTTACCTCCTGAGCT	TAGGG	0.3584	65	181	+	283	18	169837013
+FIRRM	CRISPRko	GTCAGCGCTGCATACAATAGC	AGGGG	0.5677	268	182	+	81	25	169852858
+FIRRM	CRISPRko	TGGGGTGGTGCCATGAAGAAA	AAGAG	0.5216	233	183	-	116	17	169832485
+FIRRM	CRISPRko	TTCTCCAGGGCATGACTTTAT	CTGGA	0.3146	37	184	-	312	17	169832420
+FIRRM	CRISPRko	CTCAGCTAACTGTAAACAAGA	ATGGA	0.4288	138	185	-	212	9	169807888
+FIRRM	CRISPRko	CTCTGAATATTTTCCAAAATG	ATGGA	0.5509	256	186	-	94	6	169803240
+FIRRM	CRISPRko	AATCCTGGTAGCATAAAGGCT	TGGAG	0.4104	121	187	-	231	12	169827067
+FIRRM	CRISPRko	TATTCTCTTCAAAAGCAGTTA	ATGGA	0.4605	186	188	+	169	7	169804165
+FIRRM	CRISPRko	GGCCTCCTTGAAAAGAGCCTG	GAGAA	0.4808	204	189	-	152	7	169804129
+FIRRM	CRISPRko	CTTTTAATTATATGAAGGACT	GAGGA	0.3638	83	190	-	278	6	169803273
+FIRRM	CRISPRko	AGTGCCTACTGTGGTGACCTC	ATGGA	0.3734	97	191	-	265	18	169837038
+FIRRM	CRISPRko	ATCTGCTCTGTTATTTCCAGT	ATGGA	0.4264	149	192	+	213	8	169806042
+FIRRM	CRISPRko	TATAAACTTCCAAGTATTGGC	ATGAA	0.3522	77	193	-	286	8	169806066
+FIRRM	CRISPRko	GTTCCATTAACTGCTTTTGAA	GAGAA	0.6969	330	194	-	33	7	169804157
+FIRRM	CRISPRko	CATTTCCATTGAAAGCCAGGC	TGGAG	0.5542	273	195	-	92	25	169852913
+FIRRM	CRISPRko	CTCATATGAACCACCTGACAT	TGGAA	0.3728	99	196	+	266	4	169800934
+FIRRM	CRISPRko	GCTAGCAGTAAGGCAAACATA	CAGGA	0.4783	212	197	-	154	22	169849529
+FIRRM	CRISPRko	CCTTTCATCCCTCACTGTTTG	CTGAA	0.3235	61	198	+	305	14	169829439
+FIRRM	CRISPRko	AATGTGACTGTAGAAGAAGCA	AAGAG	0.6722	322	199	+	47	24	169851916
+FIRRM	CRISPRko	AGAGGACTGTCAAGCCAAAAT	TTGGA	0.4378	171	200	+	198	5	169802687
+FIRRM	CRISPRko	TAGGCCTCCTTGAAAAGAGCC	TGGAG	0.5252	258	201	-	112	7	169804131
+FIRRM	CRISPRko	AAGGCAAACATACAGGACAGG	TTGGG	0.5055	244	202	-	127	22	169849520
+FIRRM	CRISPRko	TGCTGGTGAAGCTTTGGATAC	AGGAA	0.3713	104	203	+	267	19	169842473
+FIRRM	CRISPRko	ATGGAGGCGCTCCAAGAAAGG	ATGGA	0.6658	321	204	+	51	25	169852946
+FIRRM	CRISPRko	CAGCCTGGCTTTCAATGGAAA	TGGAG	0.5483	277	205	+	95	25	169852926
+FIRRM	CRISPRko	GGACAGTTTGTTCCCTAAGCT	CAGGA	0.2683	47	206	-	326	18	169837015
+FIRRM	CRISPRko	ATAAGTGGATCCAGTGTCACT	AGGAA	0.4338	169	207	-	206	12	169827126
+FIRRM	CRISPRko	AATGCTGACTACAGATTATTT	CAGAA	0.3224	69	208	+	306	10	169821700
+FIRRM	CRISPRko	GAACTGCTGGACATGGTTTGC	ATGGA	0.4078	140	209	+	235	7	169804189
+FIRRM	CRISPRko	GATGAAACTGAAGCTGCCAAA	GTGGA	0.6333	317	210	+	59	24	169851847
+FIRRM	CRISPRko	GCCTGATGGGGTGGTGCCATG	AAGAA	0.3896	131	211	-	248	17	169832491
+FIRRM	CRISPRko	ACTGCAGAATGCAGGAAATGG	CTGAG	0.5089	259	212	+	123	18	169837071
+FIRRM	CRISPRko	TGGCTGAGCAGGAGTCGTACT	TTGGG	0.3466	92	213	+	291	18	169837089
+FIRRM	CRISPRko	TTTGAATGAAAAATCCCAACA	GTGGA	0.4605	214	214	-	170	20	169843726
+FIRRM	CRISPRko	CATGCATTTCATGCCAATACT	TGGAA	0.3088	71	215	+	314	8	169806069
+FIRRM	CRISPRko	TGCTTCTTTTGGGGAAAATTT	CTGGA	0.4835	241	216	-	148	18	169836945
+FIRRM	CRISPRko	ACCTTCTCCAGGCTCTTTTCA	AGGAG	0.4509	209	217	+	182	7	169804136
+FIRRM	CRISPRko	ATTTACAGGGATTAAAGAGTA	AGGGG	0.4314	184	218	+	209	14	169829349
+FIRRM	CRISPRko	CTGGAAGGAAATATGTTGCCA	CAGAG	0.3882	144	219	-	250	18	169836981
+FIRRM	CRISPRko	GATGATATCCTTCCTTGCCTG	ATGGG	0.4396	202	220	-	195	17	169832507
+FIRRM	CRISPRko	GTAGATGACAATGATGATATT	TTGAA	0.4698	235	221	+	162	7	169804222
+FIRRM	CRISPRko	TTGAATGAAAAATCCCAACAG	TGGAA	0.4648	231	222	-	167	20	169843725
+FIRRM	CRISPRko	CATTTACAGGGATTAAAGAGT	AAGGG	0.4306	189	223	+	210	14	169829348
+FIRRM	CRISPRko	CATGGCACCACCCCATCAGGC	AAGGA	0.4377	201	224	+	200	17	169832506
+FIRRM	CRISPRko	ACAGACAATGGTGCAGCTCTT	AGGAG	0.4241	187	225	+	214	6	169803183
+FIRRM	CRISPRko	ACGTTACATACATACTCTAGG	GTGAA	0.4991	269	226	+	133	25	169852981
+FIRRM	CRISPRko	CACAGACAATGGTGCAGCTCT	TAGGA	0.3656	126	227	+	276	6	169803182
+FIRRM	CRISPRko	TAGCTGACAGGAGTTGGCTGC	TAGAA	0.3711	135	228	+	268	22	169849561
+FIRRM	CRISPRko	TTCTTCCCAGGGGAACTCCTG	ACGAG	0.5582	320	229	-	85	25	169852814
+FIRRM	CRISPRko	CAGGCGTTACCTCCTGAGCTT	AGGGA	0.3511	116	230	+	289	18	169837014
+FIRRM	CRISPRko	GTTCAGCATATCTGTGCCACA	CAGGA	0.4392	208	231	+	197	6	169803223
+FIRRM	CRISPRko	GCCACACAGGAATCCATCATT	TTGGA	0.3834	151	232	+	257	6	169803238
+FIRRM	CRISPRko	TGATGAGTTGATAACATTCTC	CAGGG	0.354	125	233	-	285	17	169832436
+FIRRM	CRISPRko	TTTTTTAAACATTAAACAGGT	AAGGA	0.2467	84	234	+	327	19	169842539
+FIRRM	CRISPRko	CACAGTAGGCACTGCAGAATG	CAGGA	0.4082	179	235	+	234	18	169837061
+FIRRM	CRISPRko	GAGCTCGTCAGGAGTTCCCCT	GGGAA	0.5285	310	236	+	106	25	169852821
+FIRRM	CRISPRko	TCAGCTAACTGTAAACAAGAA	TGGAA	0.367	145	237	-	274	9	169807887
+FIRRM	CRISPRko	CAAAAGCAGTTAATGGAACTG	CTGGA	0.3917	175	238	+	245	7	169804174
+FIRRM	CRISPRko	AGGCGTTACCTCCTGAGCTTA	GGGAA	0.3682	152	239	+	270	18	169837015
+FIRRM	CRISPRko	TTGTTCTTAGTTTCTTCAGAA	CTGAG	0.5447	326	240	-	97	23	169850306
+FIRRM	CRISPRko	GATAAGTGGATCCAGTGTCAC	TAGGA	0.3028	107	241	-	316	12	169827127
+FIRRM	CRISPRko	TTTTTTCCAGCCTTATGCAAA	AAGAG	0.4944	288	242	+	137	25	169852798
+FIRRM	CRISPRko	AGTTTAGGATCTAGAGTTTGA	ATGAA	0.3407	133	243	-	293	20	169843742
+FIRRM	CRISPRko	TGTCTCAGTTCTGAAGAAACT	AAGAA	0.5031	301	244	+	130	23	169850313
+FIRRM	CRISPRko	TTTACAGGGATTAAAGAGTAA	GGGGA	0.4163	218	245	+	226	14	169829350
+FIRRM	CRISPRko	AAATTTCTGGATAAACTCCAG	CTGAA	0.4007	207	246	-	239	18	169836930
+FIRRM	CRISPRko	TGAAAAGAGCCTGGAGAAGGT	CTGAA	0.4374	246	247	-	202	7	169804121
+FIRRM	CRISPRko	TTCCTGTGTGGCACAGATATG	CTGAA	0.3622	168	248	-	280	6	169803214
+FIRRM	CRISPRko	CCATTTCCATTGAAAGCCAGG	CTGGA	0.4736	290	249	-	159	25	169852914
+FIRRM	CRISPRko	GGAAGTAATAAATGTACACAG	ATGAA	0.3838	196	250	-	254	14	169829405
+FIRRM	CRISPRko	TTACAGGGATTAAAGAGTAAG	GGGAA	0.3769	191	251	+	262	14	169829351
+FIRRM	CRISPRko	CAGCCTTTCATGCAGGTGGTT	TTGGA	0.2831	129	252	+	324	12	169827181
+FIRRM	CRISPRko	GCAGGTGCTTTCCTAGTGACA	CTGGA	0.4377	255	253	+	199	12	169827127
+FIRRM	CRISPRko	TACAAAATCCAACATTGCCAA	ACGAA	0.5076	331	254	-	125	21	169847747
+FIRRM	CRISPRko	GATAAGCTGCCATCTCAGCCT	AAGGA	0.4205	238	255	+	220	13	169827782
+FIRRM	CRISPRko	AGAATGCAGGAAATGGCTGAG	CAGGA	0.4343	254	256	+	205	18	169837076
+FIRRM	CRISPRko	TCCATTCTTGTTTACAGTTAG	CTGAG	0.3634	180	257	+	279	9	169807898
+FIRRM	CRISPRko	CAAAGCAGACAGTACTGTGTT	CTGAA	0.2727	136	258	-	325	19	169842420
+FIRRM	CRISPRko	AGAAATTTTCCCCAAAAGAAG	CAGAA	0.3939	219	259	+	242	18	169836958
+FIRRM	CRISPRko	AGCAGACAGTACTGTGTTCTG	AAGGA	0.3615	183	260	-	281	19	169842417
+FIRRM	CRISPRko	CAATGGAAATGGAGGCGCTCC	AAGAA	0.4753	309	261	+	156	25	169852938
+FIRRM	CRISPRko	CCTGGCAGACTGGGTTTGTAG	ATGAA	0.4463	274	262	+	191	24	169851827
+FIRRM	CRISPRko	GACCTGTACTCTTCTTCCCAG	GGGAA	0.4867	323	263	-	146	25	169852825
+FIRRM	CRISPRko	AGAGCTCGTCAGGAGTTCCCC	TGGGA	0.4706	308	264	+	161	25	169852820
+FIRRM	CRISPRko	CCTTATGCAAAAAGAGCTCGT	CAGGA	0.432	261	265	+	208	25	169852808
+FIRRM	CRISPRko	TTTTTAAACATTAAACAGGTA	AGGAA	0.3109	156	266	+	313	19	169842540
+FIRRM	CRISPRko	AAGAGCTCGTCAGGAGTTCCC	CTGGG	0.4532	293	267	+	177	25	169852819
+FIRRM	CRISPRko	GGTGCAAAGTACAGCAAGAAT	CAGAG	0.3996	230	268	-	240	11	169823433
+FIRRM	CRISPRko	TGGCTGCTAGAACAACATACC	TTGGA	0.3386	177	269	+	296	22	169849575
+FIRRM	CRISPRko	TTTTAGCTTATCCATCCTTTC	TTGGA	0.4367	270	270	-	204	25	169852946
+FIRRM	CRISPRko	ACGACACAATTTGAGTGTTTT	CTGAA	0.2929	159	271	-	320	10	169821709
+FIRRM	CRISPRko	CCAAAGTGGAACGTGTGAAAC	AGGAA	0.4483	291	272	+	189	24	169851863
+FIRRM	CRISPRko	CTCGTCAGGAGTTCCCCTGGG	AAGAA	0.4743	325	273	+	158	25	169852824
+FIRRM	CRISPRko	CTGCTCCAGCCTGGCTTTCAA	TGGAA	0.4436	296	274	+	192	25	169852920
+FIRRM	CRISPRko	GGTCAGCGCTGCATACAATAG	CAGGG	0.4196	266	275	+	222	25	169852857
+FIRRM	CRISPRko	AAATGTGCTTTTAATTATATG	AAGGA	0.2347	160	276	-	328	6	169803280
+FIRRM	CRISPRko	TTTGCAGTTATTCATTCTTTA	TTGGA	0.3376	192	277	+	298	8	169806015
+FIRRM	CRISPRko	TTATTTCAGCTGGAGTTTATC	CAGAA	0.3049	176	278	+	315	18	169836936
+FIRRM	CRISPRko	TTCTTCAGAACTGAGACACTG	TGGAA	0.4518	315	279	-	179	23	169850295
+FIRRM	CRISPRko	GTAGAAATGTTTTTACCTCAT	ATGAA	0.287	174	280	+	322	4	169800918
+FIRRM	CRISPRko	TTCTCTTTTATATTGCAGATA	TGGGA	0.3148	193	281	+	310	16	169830696
+FIRRM	CRISPRko	CCACACAGGAATCCATCATTT	TGGAA	0.3668	228	282	+	275	6	169803239
+FIRRM	CRISPRko	AGACTGGGTTTGTAGATGAAA	CTGAA	0.4178	281	283	+	224	24	169851833
+FIRRM	CRISPRko	TGCATACAATAGCAGGGGCTT	TGGAA	0.4206	287	284	+	219	25	169852866
+FIRRM	CRISPRko	ACAGTTTTGAGCAGTGTTCTG	GTGAA	0.4057	271	285	+	236	14	169829307
+FIRRM	CRISPRko	TGTCTTCTTCTGGTTGTTGTC	ATGGA	0.3303	206	286	+	302	13	169827758
+FIRRM	CRISPRko	AACAGGAAAAAGGTATTTTCT	GGGAA	0.4472	319	287	+	190	24	169851881
+FIRRM	CRISPRko	CTATTTCCTCTTGGTGTGCTT	TAGAA	0.3364	211	288	-	299	12	169827092
+FIRRM	CRISPRko	TGTTTCAACTGGCTTTTTATT	ATGGA	0.3508	221	289	-	290	9	169807818
+FIRRM	CRISPRko	CTTGATTTGTTTTTACAGACT	GTGAA	0.3406	217	290	+	294	5	169802621
+FIRRM	CRISPRko	GAAACAGGAAAAAGGTATTTT	CTGGG	0.4393	318	291	+	196	24	169851879
+FIRRM	CRISPRko	GCCAAAGTGGAACGTGTGAAA	CAGGA	0.4223	300	292	+	215	24	169851862
+FIRRM	CRISPRko	TTTCTTCAGAACTGAGACACT	GTGGA	0.4186	295	293	-	223	23	169850296
+FIRRM	CRISPRko	GTCACATTAGCAAAGGGTTCC	CAGAA	0.4155	292	294	-	227	24	169851888
+FIRRM	CRISPRko	GCATTCTTCTTACCTGTGTAT	CTGAA	0.3516	232	295	-	288	11	169823468
+FIRRM	CRISPRko	AACCCTTTGCTAATGTGACTG	TAGAA	0.404	285	296	+	237	24	169851905
+FIRRM	CRISPRko	ACCTTTGGTAACACTTGTGAA	AAGAA	0.2141	194	297	-	330	4	169800956
+FIRRM	CRISPRko	ACGAGCTCTTTTTGCATAAGG	CTGGA	0.3836	275	298	-	256	25	169852793
+FIRRM	CRISPRko	GTTGGATTTTGTATCTTCTTT	AGGAA	0.3393	236	299	+	295	21	169847770
+FIRRM	CRISPRko	CGAGCTCTTTTTGCATAAGGC	TGGAA	0.368	265	300	-	271	25	169852792
+FIRRM	CRISPRko	TTCCACAGTGTCTCAGTTCTG	AAGAA	0.3921	297	301	+	244	23	169850305
+FIRRM	CRISPRko	TGACCTGTACTCTTCTTCCCA	GGGGA	0.4125	314	302	-	229	25	169852826
+FIRRM	CRISPRko	CAGCAGTTCCATTAACTGCTT	TTGAA	0.3005	226	303	-	318	7	169804162
+FIRRM	CRISPRko	TTTAGCTTATCCATCCTTTCT	TGGAG	0.3952	304	304	-	241	25	169852945
+FIRRM	CRISPRko	CCTTTGCTAATGTGACTGTAG	AAGAA	0.3685	276	305	+	269	24	169851908
+FIRRM	CRISPRko	ATGTAAAGAAAGTACCTTCTC	CAGAA	0.3783	289	306	-	260	23	169850343
+FIRRM	CRISPRko	CTGCATACAATAGCAGGGGCT	TTGGA	0.367	279	307	+	273	25	169852865
+FIRRM	CRISPRko	GCAGATTTTCTGCTTCTTTTG	GGGAA	0.3307	252	308	-	301	18	169836955
+FIRRM	CRISPRko	GACCTTCTCCAGGCTCTTTTC	AAGGA	0.3264	251	309	+	304	7	169804135
+FIRRM	CRISPRko	AGGCAGATTTTCTGCTTCTTT	TGGGG	0.3167	248	310	-	308	18	169836957
+FIRRM	CRISPRko	CCAGGCTGGAGCAGGACCCTT	TTGGA	0.3787	299	311	-	259	25	169852898
+FIRRM	CRISPRko	CTGACCTGTACTCTTCTTCCC	AGGGG	0.3522	272	312	-	287	25	169852827
+FIRRM	CRISPRko	GGCAGATTTTCTGCTTCTTTT	GGGGA	0.3197	253	313	-	307	18	169836956
+FIRRM	CRISPRko	CTTGCCTGATGGGGTGGTGCC	ATGAA	0.3024	243	314	-	317	17	169832494
+FIRRM	CRISPRko	GCTGACCTGTACTCTTCTTCC	CAGGG	0.3871	313	315	-	251	25	169852828
+FIRRM	CRISPRko	ATGAAACTGAAGCTGCCAAAG	TGGAA	0.4035	328	316	+	238	24	169851848
+FIRRM	CRISPRko	CCATTGAAAGCCAGGCTGGAG	CAGGA	0.3927	324	317	-	243	25	169852908
+FIRRM	CRISPRko	GAGGCAGATTTTCTGCTTCTT	TTGGG	0.2972	249	318	-	319	18	169836958
+FIRRM	CRISPRko	CCAGCCTGGCTTTCAATGGAA	ATGGA	0.3292	267	319	+	303	25	169852925
+FIRRM	CRISPRko	AAACAGGAAAAAGGTATTTTC	TGGGA	0.3757	307	320	+	263	24	169851880
+FIRRM	CRISPRko	TAGTTCCACAGTGTCTCAGTT	CTGAA	0.3433	278	321	+	292	23	169850302
+FIRRM	CRISPRko	AAAGAGGTCATCTTTACAGGT	ATGGG	0.3777	316	322	+	261	24	169851936
+FIRRM	CRISPRko	TGTTGGATTTTGTATCTTCTT	TAGGA	0.1205	247	323	+	331	21	169847769
+FIRRM	CRISPRko	CACAAATGTGCTTTTAATTAT	ATGAA	0.2864	257	324	-	323	6	169803283
+FIRRM	CRISPRko	AGAACAAAGTTGTATCCTTTC	TGGAG	0.3549	298	325	+	284	23	169850335
+FIRRM	CRISPRko	CCTGCTCCAGCCTGGCTTTCA	ATGGA	0.338	306	326	+	297	25	169852919
+FIRRM	CRISPRko	CTTGGAGCGCCTCCATTTCCA	TTGAA	0.3337	311	327	-	300	25	169852926
+FIRRM	CRISPRko	AAGAACAAAGTTGTATCCTTT	CTGGA	0.3162	303	328	+	309	23	169850334
+FIRRM	CRISPRko	AACAAAGTTGTATCCTTTCTG	GAGAA	0.3147	302	329	+	311	23	169850337

resources/KO_SA_mouse.txt

@@ -0,0 +1,1001 @@
+Target Gene Symbol	CRISPR Mechanism	sgRNA Sequence	PAM Sequence	On-Target Efficacy Score	Off-Target Rank	Combined Rank	Strand of sgRNA	On-Target Rank	Exon Number	sgRNA Cut Position (1-based)
+Gnai3	CRISPRko	TCAGCAAGATGTTCTTCGGAC	AAGAG	0.8422	4	1	-	4	5	108115826
+Gnai3	CRISPRko	TCTTGTCCGAAGAACATCTTG	CTGAG	0.9097	17	2	+	1	5	108115837
+Gnai3	CRISPRko	CCAAAGATCCGAACGAAAAAA	GTGGA	0.8236	25	3	-	6	6	108112567
+Gnai3	CRISPRko	CCAGTTTGAAGATCTGAACCG	AAGAA	0.5763	6	4	-	34	8	108109555
+Gnai3	CRISPRko	GATTACGACCTTGTTCTGGCT	GAGGA	0.5982	15	5	-	28	6	108112491
+Gnai3	CRISPRko	AGGACGGGGAGAAAGCGGCCA	AAGAA	0.6774	23	6	-	21	1	108145915
+Gnai3	CRISPRko	TGAACCGAAGAAAAGATACCA	AGGAG	0.6721	26	7	-	23	8	108109541
+Gnai3	CRISPRko	CAGGCTATGACGTACTTGAAG	TAGAG	0.82	42	8	+	7	5	108115766
+Gnai3	CRISPRko	CAGTGAATCCACTTTTTTCGT	TCGGA	0.542	7	9	+	44	6	108112570
+Gnai3	CRISPRko	ATCATTGCAATCATACGAGCC	ATGGG	0.5257	2	10	-	49	3	108123587
+Gnai3	CRISPRko	ACTTACTATGAAGCAGAATCA	TTGAG	0.6104	27	11	+	26	4	108118313
+Gnai3	CRISPRko	AGGCGTGATTAAACGTTTATG	GCGAG	0.5504	12	12	-	41	4	108118373
+Gnai3	CRISPRko	CGACAGTTATTTGTTTTAGCT	GGGAG	0.6766	33	13	-	22	4	108118432
+Gnai3	CRISPRko	TTAAACGTTTATGGCGAGATG	GCGGG	0.4992	3	14	-	58	4	108118365
+Gnai3	CRISPRko	CAAGATGTTCTTCGGACAAGA	GTGAA	0.5224	13	15	-	50	5	108115822
+Gnai3	CRISPRko	GTCCTCTGAATAGCCGTCCTC	ATGAA	0.696	50	16	+	17	3	108123671
+Gnai3	CRISPRko	AGGCATGCTTTAGCAGGTCCA	GGGAA	0.7089	52	17	-	16	4	108118335
+Gnai3	CRISPRko	AAATAACTGTCGGGCATCATC	CTGAG	0.5291	22	18	+	46	4	108118456
+Gnai3	CRISPRko	CAGAACAAGGTCGTAATCACT	GAGAG	0.4793	1	19	+	68	6	108112509
+Gnai3	CRISPRko	AAGGAATGTGGGCTTTATTGA	GAGGA	0.7466	60	20	-	11	8	108109425
+Gnai3	CRISPRko	GTGATTACGACCTTGTTCTGG	CTGAG	0.5049	16	21	-	56	6	108112493
+Gnai3	CRISPRko	ATGAGGACGGCTATTCAGAGG	ACGAA	0.7327	63	22	-	12	3	108123658
+Gnai3	CRISPRko	TCTTATTAAGAAAGAGAATGA	TTGAA	0.5488	37	23	+	42	7	108112016
+Gnai3	CRISPRko	TGCTGTCACTCCCTCAAAACA	GTGAA	0.7244	65	24	+	14	6	108112551
+Gnai3	CRISPRko	TGTAGGTGGCCAAAGATCCGA	ACGAA	0.5551	41	25	-	40	6	108112576
+Gnai3	CRISPRko	TATACTGTTTACATTCGTCCT	CTGAA	0.4926	20	26	+	62	3	108123655
+Gnai3	CRISPRko	GACGGTTGAAGATTGATTTTG	GGGAA	0.6925	64	27	-	19	3	108123562
+Gnai3	CRISPRko	TCATCATTAAAAACAACTTAA	AGGAA	0.7279	71	28	-	13	8	108109445
+Gnai3	CRISPRko	TAAACGTTTATGGCGAGATGG	CGGGG	0.8387	79	29	-	5	4	108118364
+Gnai3	CRISPRko	TAGCTACCTAAATGATTTGGA	TAGAA	0.5928	55	30	-	30	5	108115877
+Gnai3	CRISPRko	TCATTCATGAGGACGGCTATT	CAGAG	0.4519	8	31	-	77	3	108123664
+Gnai3	CRISPRko	ATTCATGAGGACGGCTATTCA	GAGGA	0.4515	9	32	-	79	3	108123662
+Gnai3	CRISPRko	GCCAGAACAAGGTCGTAATCA	CTGAG	0.529	43	33	+	48	6	108112507
+Gnai3	CRISPRko	GTGCCAGTTTGAAGATCTGAA	CCGAA	0.5655	58	34	-	37	8	108109558
+Gnai3	CRISPRko	ATCATACGAGCCATGGGACGG	TTGAA	0.4446	11	35	-	84	3	108123578
+Gnai3	CRISPRko	GAATCTGGTAAAAGTACCATC	GTGAA	0.5147	45	36	-	52	2	108123812
+Gnai3	CRISPRko	CCTCAGGTTCCAATACATACG	AAGAG	0.5071	44	37	-	54	8	108109601
+Gnai3	CRISPRko	AGATGATCGACCGCAACTTGC	GGGAG	0.4953	38	38	-	61	1	108145939
+Gnai3	CRISPRko	GAGTGAAGACTACAGGCATTG	TGGAG	0.6945	82	39	-	18	5	108115803
+Gnai3	CRISPRko	GGACGGTTGAAGATTGATTTT	GGGGA	0.447	19	40	-	81	3	108123563
+Gnai3	CRISPRko	TTCTTAATAAGAAAGACCTTT	TTGAG	0.4665	31	41	-	72	7	108111992
+Gnai3	CRISPRko	CCTCTTCGTATGTATTGGAAC	CTGAG	0.506	49	42	+	55	8	108109610
+Gnai3	CRISPRko	TGAGTTGGAATGTAGTTGGTC	TGGGA	0.5175	56	43	+	51	5	108115859
+Gnai3	CRISPRko	CAAGATGATCGACCGCAACTT	GCGGG	0.5838	74	44	-	33	1	108145941
+Gnai3	CRISPRko	CAGGCATGCTTTAGCAGGTCC	AGGGA	0.4408	21	45	-	87	4	108118336
+Gnai3	CRISPRko	CTGAACCGAAGAAAAGATACC	AAGGA	0.5075	57	46	-	53	8	108109542
+Gnai3	CRISPRko	TTGTTTTTCAGAACCGAATGC	ATGAA	0.4869	48	47	-	64	7	108112082
+Gnai3	CRISPRko	ATGATCGACCGCAACTTGCGG	GAGGA	0.4652	39	48	-	73	1	108145937
+Gnai3	CRISPRko	AGAGTGAAGACTACAGGCATT	GTGGA	0.4913	54	49	-	63	5	108115804
+Gnai3	CRISPRko	CTCCTTTTTGAAGAATCATTC	ATGAG	0.503	61	50	-	57	3	108123679
+Gnai3	CRISPRko	AAGGTCTTTCTTATTAAGAAA	GAGAA	0.6257	96	51	+	24	7	108112008
+Gnai3	CRISPRko	ATAACTGTCGGGCATCATCCT	GAGAA	0.4435	35	52	+	85	4	108118458
+Gnai3	CRISPRko	TGGCCACCTACATCAAACATT	CTGAG	0.7193	108	53	+	15	6	108112600
+Gnai3	CRISPRko	GATTGATTTTGGGGAATCTGC	CAGAG	0.5558	84	54	-	39	3	108123552
+Gnai3	CRISPRko	TCGACCGCAACTTGCGGGAGG	ACGGG	0.5424	81	55	-	43	1	108145933
+Gnai3	CRISPRko	CTGCCTCAGGTTCCAATACAT	ACGAA	0.4052	18	56	-	109	8	108109604
+Gnai3	CRISPRko	TTGTTTTTTTTTTAGGCGCTG	GAGAA	0.5636	91	57	-	38	2	108123835
+Gnai3	CRISPRko	GTTCAGATCTTCAAACTGGCA	CTGAA	0.6885	110	58	+	20	8	108109572
+Gnai3	CRISPRko	TTATTTAGCTACCTAAATGAT	TTGGA	0.529	83	59	-	47	5	108115882
+Gnai3	CRISPRko	TTTAGCTGGGAGTGCTGAAGA	AGGAG	0.8753	127	60	-	3	4	108118418
+Gnai3	CRISPRko	ATGGCTCGTATGATTGCAATG	ATGGA	0.3567	5	61	+	125	3	108123600
+Gnai3	CRISPRko	CTTTTTGAGGAAAAAATAAAG	AGGAG	0.8024	124	62	-	8	7	108111975
+Gnai3	CRISPRko	CCTTTTTGAGGAAAAAATAAA	GAGGA	0.5737	97	63	-	35	7	108111976
+Gnai3	CRISPRko	GACCTTTTTGAGGAAAAAATA	AAGAG	0.5973	104	64	-	29	7	108111978
+Gnai3	CRISPRko	TATTTGTTTTAGCTGGGAGTG	CTGAA	0.4243	32	65	-	101	4	108118425
+Gnai3	CRISPRko	GACCGCAACTTGCGGGAGGAC	GGGGA	0.4955	73	66	-	60	1	108145931
+Gnai3	CRISPRko	TCGTATGATTGCAATGATGGA	CTGAA	0.5908	103	67	+	32	3	108123605
+Gnai3	CRISPRko	CCTTTTTGAAGAATCATTCAT	GAGGA	0.4166	30	68	-	105	3	108123677
+Gnai3	CRISPRko	GGTGTCTGTGGCACAGGTGAA	GTGAG	0.7793	126	69	+	10	8	108109518
+Gnai3	CRISPRko	CCCAGCTAAAACAAATAACTG	TCGGG	0.4194	34	70	+	102	4	108118444
+Gnai3	CRISPRko	TCATTGCAATCATACGAGCCA	TGGGA	0.3608	14	71	-	124	3	108123586
+Gnai3	CRISPRko	ACAGGCATGCTTTAGCAGGTC	CAGGG	0.441	53	72	-	86	4	108118337
+Gnai3	CRISPRko	GTTTAATCACGCCTGCTAGTT	CTGAA	0.3501	10	73	+	129	4	108118395
+Gnai3	CRISPRko	GTAGAGTTCCTTGAAGGTGAA	GTGGG	0.7865	132	74	+	9	5	108115786
+Gnai3	CRISPRko	CCGAAGAACATCTTGCTGAGT	TGGAA	0.6001	114	75	+	27	5	108115843
+Gnai3	CRISPRko	CCATGGGCTGCACGTTGAGCG	CCGAG	0.5713	105	76	-	36	1	108145990
+Gnai3	CRISPRko	AAAGTGGATTCACTGTTTTGA	GGGAG	0.8984	142	77	-	2	6	108112549
+Gnai3	CRISPRko	AAAAGGTCTTTCTTATTAAGA	AAGAG	0.3827	29	78	+	117	7	108112006
+Gnai3	CRISPRko	ATTCTGGATAACAGATTGTTA	ATGGA	0.3618	24	79	+	123	7	108111959
+Gnai3	CRISPRko	GCAGCTGCCTCTTCGTATGTA	TTGGA	0.4086	40	80	+	108	8	108109603
+Gnai3	CRISPRko	CAGAACCGAATGCATGAAAGC	ATGAA	0.472	80	81	-	71	7	108112074
+Gnai3	CRISPRko	CGCAACTTGCGGGAGGACGGG	GAGAA	0.4636	76	82	-	75	1	108145928
+Gnai3	CRISPRko	GGGACGGTTGAAGATTGATTT	TGGGG	0.4459	70	83	-	83	3	108123564
+Gnai3	CRISPRko	CACGCCCTTTACCTGTGTATT	CTGGA	0.4827	87	84	+	67	7	108111941
+Gnai3	CRISPRko	GTCCATTAACAATCTGTTATC	CAGAA	0.418	51	85	-	103	7	108111950
+Gnai3	CRISPRko	TTAAAAACAACTTAAAGGAAT	GTGGG	0.3787	36	86	-	119	8	108109439
+Gnai3	CRISPRko	TCAGTCCATCATTGCAATCAT	ACGAG	0.451	75	87	-	80	3	108123594
+Gnai3	CRISPRko	TAAAGGAATGTGGGCTTTATT	GAGAG	0.4362	66	88	-	91	8	108109427
+Gnai3	CRISPRko	CAGTTTGTTTTTGATGCTGTT	ACGGA	0.5911	128	89	-	31	8	108109473
+Gnai3	CRISPRko	AGCGCCGAGGACAAGGCGGCG	GTGGA	0.6174	136	90	-	25	1	108145973
+Gnai3	CRISPRko	TTTTGTTTTTTTTTTAGGCGC	TGGAG	0.3251	28	91	-	134	2	108123837
+Gnai3	CRISPRko	AAAAGTACCATCGTGAAACAG	ATGAA	0.4964	107	92	-	59	2	108123803
+Gnai3	CRISPRko	GGGGAGAAAGCGGCCAAAGAA	GTGAA	0.5329	122	93	-	45	1	108145910
+Gnai3	CRISPRko	ACCGCAACTTGCGGGAGGACG	GGGAG	0.4858	106	94	-	65	1	108145930
+Gnai3	CRISPRko	AAGATGATCGACCGCAACTTG	CGGGA	0.4139	67	95	-	107	1	108145940
+Gnai3	CRISPRko	GTTATCCAGAATACACAGGTA	AAGGG	0.3915	62	96	-	113	7	108111935
+Gnai3	CRISPRko	TGTAGTCTTCACTCTTGTCCG	AAGAA	0.3464	47	97	+	130	5	108115825
+Gnai3	CRISPRko	CATGCTTTCATGCATTCGGTT	CTGAA	0.3214	46	98	+	135	7	108112086
+Gnai3	CRISPRko	TTTTTGGTGTCTGTGGCACAG	GTGAA	0.4832	117	99	+	66	8	108109513
+Gnai3	CRISPRko	TGGGACGGTTGAAGATTGATT	TTGGG	0.3884	69	100	-	115	3	108123565
+Gnai3	CRISPRko	CTGAAGAAGGAGTCATGACTT	CAGAA	0.4324	90	101	-	95	4	108118404
+Gnai3	CRISPRko	CGACCGCAACTTGCGGGAGGA	CGGGG	0.4312	89	102	-	96	1	108145932
+Gnai3	CRISPRko	GTGGAGACCCACTTCACCTTC	AAGGA	0.464	112	103	-	74	5	108115783
+Gnai3	CRISPRko	AAAAAATCACTTACTATGAAG	CAGAA	0.4294	88	104	+	98	4	108118305
+Gnai3	CRISPRko	ATGGGCTGCACGTTGAGCGCC	GAGGA	0.4388	98	105	-	89	1	108145988
+Gnai3	CRISPRko	GAAAAAAGTGGATTCACTGTT	TTGAG	0.475	120	106	-	70	6	108112553
+Gnai3	CRISPRko	GAATCATTGAGCTGATATTCC	CTGGA	0.392	78	107	+	112	4	108118328
+Gnai3	CRISPRko	AAAAGTGGATTCACTGTTTTG	AGGGA	0.4516	113	108	-	78	6	108112550
+Gnai3	CRISPRko	CTTAAAGGAATGTGGGCTTTA	TTGAG	0.414	92	109	-	106	8	108109429
+Gnai3	CRISPRko	TTGAAGTAGAGTTCCTTGAAG	GTGAA	0.4465	116	110	+	82	5	108115781
+Gnai3	CRISPRko	CCGACAGTTATTTGTTTTAGC	TGGGA	0.2955	59	111	-	139	4	108118433
+Gnai3	CRISPRko	GCGCCGAGGACAAGGCGGCGG	TGGAG	0.4764	133	112	-	69	1	108145972
+Gnai3	CRISPRko	GCCGCCGCCATGGGCTGCACG	TTGAG	0.4173	99	113	-	104	1	108145997
+Gnai3	CRISPRko	CTGAGTTGGAATGTAGTTGGT	CTGGG	0.3791	86	114	+	118	5	108115858
+Gnai3	CRISPRko	ACGTACTTGAAGTAGAGTTCC	TTGAA	0.3768	85	115	+	120	5	108115775
+Gnai3	CRISPRko	GTCATCATTAAAAACAACTTA	AAGGA	0.439	118	116	-	88	8	108109446
+Gnai3	CRISPRko	GCCTGTAGTCTTCACTCTTGT	CCGAA	0.3906	93	117	+	114	5	108115822
+Gnai3	CRISPRko	CTCAAAAAGGTCTTTCTTATT	AAGAA	0.2564	68	118	+	140	7	108112002
+Gnai3	CRISPRko	GTGAAGCTGCTGCTGCTCGGT	GAGGG	0.4339	119	119	-	92	1	108145889
+Gnai3	CRISPRko	CAGCTGCCTCTTCGTATGTAT	TGGAA	0.3207	77	120	+	136	8	108109604
+Gnai3	CRISPRko	GTTTTGTTTTTTTTTTAGGCG	CTGGA	0.2286	72	121	-	141	2	108123838
+Gnai3	CRISPRko	TTTTAGCTGGGAGTGCTGAAG	AAGGA	0.4334	121	122	-	93	4	108118419
+Gnai3	CRISPRko	AAGTGAAGCTGCTGCTGCTCG	GTGAG	0.4607	141	123	-	76	1	108145891
+Gnai3	CRISPRko	CGAGGACAAGGCGGCGGTGGA	GCGGA	0.4368	129	124	-	90	1	108145968
+Gnai3	CRISPRko	CCCGACAGTTATTTGTTTTAG	CTGGG	0.3517	94	125	-	128	4	108118434
+Gnai3	CRISPRko	TTGTTTTAGCTGGGAGTGCTG	AAGAA	0.3641	102	126	-	122	4	108118422
+Gnai3	CRISPRko	CTGCTTACATTCAGTGCCAGT	TTGAA	0.3528	100	127	-	127	8	108109571
+Gnai3	CRISPRko	TGGAGACCCACTTCACCTTCA	AGGAA	0.4278	130	128	-	100	5	108115782
+Gnai3	CRISPRko	GACCTTGTTCTGGCTGAGGAT	GAGGA	0.43	134	129	-	97	6	108112485
+Gnai3	CRISPRko	GAGGACAAGGCGGCGGTGGAG	CGGAG	0.4333	138	130	-	94	1	108145967
+Gnai3	CRISPRko	ACGACCTTGTTCTGGCTGAGG	ATGAG	0.4278	137	131	-	99	6	108112487
+Gnai3	CRISPRko	TCAATCATTCTCTTTCTTAAT	AAGAA	0.1657	95	132	-	142	7	108112005
+Gnai3	CRISPRko	TCCGAAGAACATCTTGCTGAG	TTGGA	0.298	101	133	+	138	5	108115842
+Gnai3	CRISPRko	TTAATAAGAAAGACCTTTTTG	AGGAA	0.3555	115	134	-	126	7	108111989
+Gnai3	CRISPRko	TTGATGTAGGTGGCCAAAGAT	CCGAA	0.3358	109	135	-	133	6	108112580
+Gnai3	CRISPRko	TCCAACTCAGCAAGATGTTCT	TCGGA	0.3456	111	136	-	131	5	108115832
+Gnai3	CRISPRko	TGAAGCTGCTGCTGCTCGGTG	AGGGG	0.4042	135	137	-	110	1	108145888
+Gnai3	CRISPRko	ATTCAGTGCCAGTTTGAAGAT	CTGAA	0.3841	131	138	-	116	8	108109563
+Gnai3	CRISPRko	ACCTTGTTCTGGCTGAGGATG	AGGAA	0.4005	140	139	-	111	6	108112484
+Gnai3	CRISPRko	AAAAAGTGGATTCACTGTTTT	GAGGG	0.3425	125	140	-	132	6	108112551
+Gnai3	CRISPRko	CTTAATAAGAAAGACCTTTTT	GAGGA	0.3144	123	141	-	137	7	108111990
+Gnai3	CRISPRko	TACCATTTCCTCATCCTCAGC	CAGAA	0.3762	139	142	+	121	6	108112488
+Pbsn	CRISPRko	AATGTACAGCGTATCATGGAC	ACGGG	0.7497	2	1	-	8	5	77842522
+Pbsn	CRISPRko	AAATGATGTCACACGAGTGGC	TGGAG	0.788	5	2	-	5	4	77842911
+Pbsn	CRISPRko	AGACAGTCGCTCACACATGAT	GAGGG	0.7815	8	3	-	6	1	77853482
+Pbsn	CRISPRko	GTGCTAGGCGTCTCCAGTGTG	ATGAG	0.7926	25	4	-	3	1	77853430
+Pbsn	CRISPRko	TTCTCATTTACCATCTTTAAC	ATGAA	0.6043	16	5	+	15	4	77842983
+Pbsn	CRISPRko	AAGAAGTTTACTATGCACAGT	GTGAG	0.7899	27	6	-	4	3	77845022
+Pbsn	CRISPRko	GAACAGAAGAAATGATGTCAC	ACGAG	0.8312	35	7	-	2	4	77842920
+Pbsn	CRISPRko	TGAATGCTATGCTCCCTTCAT	CTGAG	0.5002	9	8	+	30	4	77843005
+Pbsn	CRISPRko	AAGATAAATGAAGGCTCACCA	TTGAG	0.6594	28	9	-	11	2	77848049
+Pbsn	CRISPRko	AAAGGCAAACAACTGAATAAG	GAGGA	0.5247	14	10	-	27	5	77842597
+Pbsn	CRISPRko	GAAATGATGTCACACGAGTGG	CTGGA	0.5774	21	11	-	20	4	77842912
+Pbsn	CRISPRko	GGATGATGACCCTCATCATGT	GTGAG	0.53	19	12	+	26	1	77853483
+Pbsn	CRISPRko	GAAGAAATGGGCATTGAGGAA	GAGAA	0.7089	37	13	-	9	5	77842546
+Pbsn	CRISPRko	GTTTGCCAAGGCCCGTCAATC	TGGAA	0.4507	4	14	+	42	2	77848113
+Pbsn	CRISPRko	ACTTAGTGGAAGAAATGGGCA	TTGAG	0.6459	38	15	-	12	5	77842554
+Pbsn	CRISPRko	CGGAGTTCATGAACTTAGTGG	AAGAA	0.5913	33	16	-	17	5	77842566
+Pbsn	CRISPRko	GACGGAAGTAGGTTCTCAATG	GTGAG	0.5416	26	17	+	24	2	77848045
+Pbsn	CRISPRko	GAGACGCCTAGCACATGCAGT	GTGAG	0.4972	20	18	+	32	1	77853452
+Pbsn	CRISPRko	GTGCCAACTGTATAAAGTCAT	AGGAA	0.4481	10	19	-	43	3	77845057
+Pbsn	CRISPRko	TGACGGAGTTCATGAACTTAG	TGGAA	0.494	22	20	-	33	5	77842569
+Pbsn	CRISPRko	TCTCCTCCCACACAAAATGTG	ACGGA	0.446	11	21	+	44	2	77848025
+Pbsn	CRISPRko	GAAGAGAATGTACAGCGTATC	ATGGA	0.4265	3	22	-	55	5	77842528
+Pbsn	CRISPRko	AACGCAGCAAAAGGCAAACAA	CTGAA	0.7764	53	23	-	7	5	77842606
+Pbsn	CRISPRko	GTCTTACCTATCTCAGATGAA	GGGAG	0.4998	29	24	-	31	4	77843006
+Pbsn	CRISPRko	ATGTCTTACCTATCTCAGATG	AAGGG	0.4454	15	25	-	46	4	77843008
+Pbsn	CRISPRko	TCTTTTTGAGACTCATCACAC	TGGAG	0.5776	44	26	+	19	1	77853429
+Pbsn	CRISPRko	ATGACGGAGTTCATGAACTTA	GTGGA	0.5348	41	27	-	25	5	77842570
+Pbsn	CRISPRko	AACTGAATAAGGAGGAGATGA	CGGAG	0.98	65	28	-	1	5	77842587
+Pbsn	CRISPRko	GTCACACGAGTGGCTGGAGTT	TTGGG	0.6853	56	29	-	10	4	77842904
+Pbsn	CRISPRko	TACTTCCGTCACATTTTGTGT	GGGAG	0.4267	12	30	-	54	2	77848019
+Pbsn	CRISPRko	TGTTTGCCAAGGCCCGTCAAT	CTGGA	0.3569	1	31	+	65	2	77848112
+Pbsn	CRISPRko	AGAGACAGTCGCTCACACATG	ATGAG	0.3891	7	32	-	61	1	77853484
+Pbsn	CRISPRko	TTCATGAACTTAGTGGAAGAA	ATGGG	0.509	40	33	-	29	5	77842561
+Pbsn	CRISPRko	CTCCTCCCACACAAAATGTGA	CGGAA	0.4756	32	34	+	37	2	77848026
+Pbsn	CRISPRko	CAAAAGGCAAACAACTGAATA	AGGAG	0.557	48	35	-	22	5	77842599
+Pbsn	CRISPRko	AGTGCCAACTGTATAAAGTCA	TAGGA	0.4057	13	36	-	59	3	77845058
+Pbsn	CRISPRko	AAGGCAAACAACTGAATAAGG	AGGAG	0.6445	62	37	-	13	5	77842596
+Pbsn	CRISPRko	TTCCGTCACATTTTGTGTGGG	AGGAG	0.49	43	38	-	34	2	77848016
+Pbsn	CRISPRko	CTACTTCCGTCACATTTTGTG	TGGGA	0.3193	6	39	-	71	2	77848020
+Pbsn	CRISPRko	AGTTGGCACTTAGTCCCTTTC	CTGAA	0.4381	31	40	+	49	3	77845084
+Pbsn	CRISPRko	TCCAGTGTGATGAGTCTCAAA	AAGAA	0.4355	30	41	-	50	1	77853418
+Pbsn	CRISPRko	CATTCATGTTAAAGATGGTAA	ATGAG	0.5799	63	42	-	18	4	77842975
+Pbsn	CRISPRko	TAGCACATGCAGTGTGAGCAG	GAGGA	0.6392	68	43	+	14	1	77853460
+Pbsn	CRISPRko	TCTTCTGTTCTTGTTAAAATA	ATGAA	0.3557	17	44	+	66	4	77842946
+Pbsn	CRISPRko	TAGTGGAAGAAATGGGCATTG	AGGAA	0.5944	69	45	-	16	5	77842551
+Pbsn	CRISPRko	CCAACTGTATAAAGTCATAGG	AAGGA	0.4289	34	46	-	51	3	77845054
+Pbsn	CRISPRko	CCAGTACCATGGAAAAGATAA	ATGAA	0.5237	58	47	-	28	2	77848063
+Pbsn	CRISPRko	TTCATGTTAAAGATGGTAAAT	GAGAA	0.3285	18	48	-	69	4	77842973
+Pbsn	CRISPRko	GATAAATGAAGGCTCACCATT	GAGAA	0.4874	55	49	-	35	2	77848047
+Pbsn	CRISPRko	CTTCCGTCACATTTTGTGTGG	GAGGA	0.3315	24	50	-	68	2	77848017
+Pbsn	CRISPRko	AAGGAGGAGATGACGGAGTTC	ATGAA	0.5419	70	51	-	23	5	77842579
+Pbsn	CRISPRko	CAACTGAATAAGGAGGAGATG	ACGGA	0.5651	72	52	-	21	5	77842588
+Pbsn	CRISPRko	ATATCTTACTTAATAAAAAAA	TAGAG	0.4714	54	53	+	39	2	77847984
+Pbsn	CRISPRko	TTTACTTAGCTGCCAGTACCA	TGGAA	0.4285	42	54	-	52	2	77848075
+Pbsn	CRISPRko	ATTTACTTAGCTGCCAGTACC	ATGGA	0.2796	23	55	-	72	2	77848076
+Pbsn	CRISPRko	TGGAAGAAATGGGCATTGAGG	AAGAG	0.4444	50	56	-	47	5	77842548
+Pbsn	CRISPRko	AAGTCATAGGAAGGAAAAAAC	AAGAA	0.383	36	57	-	62	3	77845043
+Pbsn	CRISPRko	TGTCTTACCTATCTCAGATGA	AGGGA	0.4282	46	58	-	53	4	77843007
+Pbsn	CRISPRko	TTAGTGGAAGAAATGGGCATT	GAGGA	0.4783	64	59	-	36	5	77842552
+Pbsn	CRISPRko	CCTAGCACATGCAGTGTGAGC	AGGAG	0.451	61	60	+	41	1	77853458
+Pbsn	CRISPRko	CACAGGTCTGCTACCTTCTTT	TTGAG	0.4732	66	61	+	38	1	77853413
+Pbsn	CRISPRko	CCCATTTCTTCCACTAAGTTC	ATGAA	0.4113	47	62	+	58	5	77842571
+Pbsn	CRISPRko	GCAAAAGGCAAACAACTGAAT	AAGGA	0.4211	49	63	-	56	5	77842600
+Pbsn	CRISPRko	CAACTGTATAAAGTCATAGGA	AGGAA	0.4459	60	64	-	45	3	77845053
+Pbsn	CRISPRko	CCTACTTCCGTCACATTTTGT	GTGGG	0.4002	45	65	-	60	2	77848021
+Pbsn	CRISPRko	TGCTGCTTTTTTTCCAGATTG	ACGGG	0.4531	67	66	-	40	2	77848114
+Pbsn	CRISPRko	TTCTTTTTGAGACTCATCACA	CTGGA	0.4387	59	67	+	48	1	77853428
+Pbsn	CRISPRko	TCATTATTTTAACAAGAACAG	AAGAA	0.4175	51	68	-	57	4	77842935
+Pbsn	CRISPRko	TTGTTGTTTCATTATTTTAAC	AAGAA	0.1923	39	69	-	74	4	77842943
+Pbsn	CRISPRko	GCCTAGCACATGCAGTGTGAG	CAGGA	0.3739	57	70	+	63	1	77853457
+Pbsn	CRISPRko	GTTTCATTATTTTAACAAGAA	CAGAA	0.2522	52	71	-	73	4	77842938
+Pbsn	CRISPRko	TCTTGCTTGGACAGTTGTCTA	AAGGA	0.3617	74	72	+	64	6	77841912
+Pbsn	CRISPRko	GTCACTAAGGTTTGATCTTGC	TTGGA	0.3437	73	73	+	67	6	77841897
+Pbsn	CRISPRko	ACGACATTTGTTTTTCAGGAA	AGGGA	0.3273	71	74	-	70	3	77845087
+Cdc45	CRISPRko	TGTCCCGTCATAACCACCGAA	ACGAG	0.6808	1	1	-	38	11	18795886
+Cdc45	CRISPRko	ATCCGAGCTATCAGTCTCTGG	GGGGA	0.7407	33	2	+	26	17	18785099
+Cdc45	CRISPRko	CTTCTCTTTGTGGCCTCGGAC	GTGGA	0.6898	24	3	-	36	3	18811341
+Cdc45	CRISPRko	GAATTTATGCTTGAACCCAAA	CTGAA	0.7723	40	4	+	21	14	18793300
+Cdc45	CRISPRko	TCATGTGTCCCGTCATAACCA	CCGAA	0.6559	20	5	-	42	11	18795891
+Cdc45	CRISPRko	CATAACCACCGAAACGAGGCG	GAGGA	0.6019	3	6	-	60	11	18795878
+Cdc45	CRISPRko	TGTTCGTGACCGATTTCCGCA	AGGAG	0.9088	59	7	-	5	2	18811547
+Cdc45	CRISPRko	TACAGACTTTCAGCATTCAGT	TTGGG	0.7923	50	8	-	15	14	18793305
+Cdc45	CRISPRko	TGCAAAGATACTCATACTCGA	AGGAG	0.6076	9	9	+	57	11	18795832
+Cdc45	CRISPRko	GCACCCGGATCTCCTTCGAGT	ATGAG	0.8225	57	10	-	9	11	18795832
+Cdc45	CRISPRko	CAGAGAAGCGCACACGGTTAG	AAGAG	0.6151	19	11	-	55	6	18807288
+Cdc45	CRISPRko	GTCATAACCACCGAAACGAGG	CGGAG	0.5751	7	12	-	70	11	18795880
+Cdc45	CRISPRko	TCCATGGACGTTTCCTTGAAG	GGGAA	0.6806	45	13	-	39	13	18794809
+Cdc45	CRISPRko	GAGACTGATAGCTCGGATAGA	AAGAA	0.57	16	14	-	71	17	18785081
+Cdc45	CRISPRko	CATGCTCTCTGTAGACTGCAC	CCGGA	0.6496	43	15	-	44	11	18795849
+Cdc45	CRISPRko	TTTGCCACCATGTCTCTAATG	GAGAG	0.818	78	16	-	11	14	18793246
+Cdc45	CRISPRko	GCATCCACGTCCGAGGCCACA	AAGAG	0.5833	27	17	+	67	3	18811348
+Cdc45	CRISPRko	CATTGTTGGACTGACAGACCA	GTGGG	0.9748	95	18	-	1	10	18797376
+Cdc45	CRISPRko	ATCTTTCAGACAGTGACGGTG	ATGGG	0.8543	93	19	-	7	6	18807324
+Cdc45	CRISPRko	AGCAGTAGAGAAAAGGGCCCT	GGGAA	0.622	51	20	+	52	15	18786959
+Cdc45	CRISPRko	AGTATACGCTGGTTCCGGTTT	CTGGG	0.6659	64	21	-	40	4	18810439
+Cdc45	CRISPRko	ACCCACAGGCCAGTGAACGTT	GTGAA	0.6994	73	22	-	32	5	18808759
+Cdc45	CRISPRko	ATGACGGGACACATGACGCTG	CAGGA	0.6636	65	23	+	41	11	18795911
+Cdc45	CRISPRko	ATACTCATACTCGAAGGAGAT	CCGGG	0.6943	77	24	+	35	11	18795839
+Cdc45	CRISPRko	CAGGAGATAGTGGAGCGAAAC	AGGAA	0.6217	60	25	-	53	7	18805423
+Cdc45	CRISPRko	ACTATTTCAGGAGATAGTGGA	GCGAA	0.5972	52	26	-	61	7	18805430
+Cdc45	CRISPRko	TATCCGAGCTATCAGTCTCTG	GGGGG	0.5575	38	27	+	79	17	18785098
+Cdc45	CRISPRko	AACTCCTTGCGGAAATCGGTC	ACGAA	0.5133	2	28	+	115	2	18811555
+Cdc45	CRISPRko	TGAGAGGTCAAAGTAATTGTG	TAGAG	0.7749	99	29	+	19	18	18784825
+Cdc45	CRISPRko	TCCTGCCTACGACGACATCTT	TAGAG	0.6339	72	30	-	46	6	18807368
+Cdc45	CRISPRko	CAGTGCTGATACAGTACCAGG	CAGAG	0.5355	28	31	+	95	12	18795218
+Cdc45	CRISPRko	ACAGGAAGAGGAGGCAGCGCA	GGGAG	0.9152	119	32	-	4	7	18805404
+Cdc45	CRISPRko	CTGTTTTCCAAGCCAGCATCC	TTGAG	0.8279	118	33	-	8	16	18786817
+Cdc45	CRISPRko	CTTGGAAAACAGGGTGACATC	TGGAG	0.7494	101	34	+	25	16	18786841
+Cdc45	CRISPRko	CAGCAGTGACACAAGAGCGTC	CAGGA	0.5134	13	35	+	114	19	18781922
+Cdc45	CRISPRko	TGCATGGGCAGAAACGACTCC	AGGAG	0.9382	129	36	-	3	12	18795120
+Cdc45	CRISPRko	CGTTTCTGCCCATGCACAGAC	CAGAG	0.5525	47	37	+	86	12	18795140
+Cdc45	CRISPRko	AGATGTCTGCTGAGCAGACTC	AAGGA	0.7188	110	38	+	28	16	18786809
+Cdc45	CRISPRko	GTGCATGGGCAGAAACGACTC	CAGGA	0.5176	29	39	-	110	12	18795121
+Cdc45	CRISPRko	GGGTAAGGAAGTCTAACCTCC	ATGAG	0.7984	132	40	+	13	15	18786931
+Cdc45	CRISPRko	TACCTTGGTCTAGAGCTCGCC	AAGAA	0.5589	69	41	-	77	15	18787032
+Cdc45	CRISPRko	TCTCTAATGGAGAGTCCTGAG	AAGGA	0.6956	112	42	-	34	14	18793234
+Cdc45	CRISPRko	TCTCGATAGCCTCTCCAGGTA	ATGGG	0.7987	135	43	-	12	14	18793178
+Cdc45	CRISPRko	CCTGGAGAGGCTATCGAGAGC	CTGGA	0.5925	85	44	+	63	14	18793195
+Cdc45	CRISPRko	GTGGTCACACTGAAACAGGGC	CTGGA	0.5275	46	45	+	102	4	18810479
+Cdc45	CRISPRko	ATAACCACCGAAACGAGGCGG	AGGAG	0.5483	62	46	-	88	11	18795877
+Cdc45	CRISPRko	AAGCGCACACGGTTAGAAGAG	GTGAG	0.7739	130	47	-	20	6	18807283
+Cdc45	CRISPRko	AAGGAAGTCTAACCTCCATGA	GTGAG	0.6237	100	48	+	51	15	18786935
+Cdc45	CRISPRko	ATCCCCCCAGAGACTGATAGC	TCGGA	0.5887	87	49	-	65	17	18785090
+Cdc45	CRISPRko	CCATGAGTGAGCAGTAGAGAA	AAGGG	0.5335	56	50	+	96	15	18786950
+Cdc45	CRISPRko	GACATCTTTAGAGATGAAGCA	GAGGA	0.7019	121	51	-	31	6	18807355
+Cdc45	CRISPRko	GGAATCTGCGAGAAATGATTG	AAGAA	0.7894	137	52	-	17	13	18794787
+Cdc45	CRISPRko	ATGTTCGTGACCGATTTCCGC	AAGGA	0.4767	5	53	-	149	2	18811548
+Cdc45	CRISPRko	CCCATGTCTGCAAGGAACTCC	TGGAG	0.9429	153	54	+	2	12	18795113
+Cdc45	CRISPRko	CCTACGACGACATCTTTAGAG	ATGAA	0.5561	81	55	-	81	6	18807363
+Cdc45	CRISPRko	GGTGTTGTACAGGCTTTCATG	GAGGG	0.5044	42	56	+	125	12	18795190
+Cdc45	CRISPRko	GAGCTGCAAAGATACTCATAC	TCGAA	0.4953	37	57	+	131	11	18795828
+Cdc45	CRISPRko	CCTCAGCCTTCAGCTCAATTA	CTGAA	0.4902	34	58	+	135	19	18781960
+Cdc45	CRISPRko	CACGTTGGCTCCACAGTTTAT	GAGGA	0.5566	91	59	+	80	5	18808849
+Cdc45	CRISPRko	TCTTTGCCACCATGTCTCTAA	TGGAG	0.5023	44	60	-	128	14	18793248
+Cdc45	CRISPRko	CTCAAGTCCTTTGTGTACTCG	GTGAG	0.4645	15	61	-	159	16	18786772
+Cdc45	CRISPRko	ATCCACGTCCGAGGCCACAAA	GAGAA	0.5158	66	62	+	112	3	18811350
+Cdc45	CRISPRko	AAGCACACAAGGCATCCACGT	CCGAG	0.6198	125	63	+	54	3	18811337
+Cdc45	CRISPRko	CTGCAAAGATACTCATACTCG	AAGGA	0.4578	12	64	+	169	11	18795831
+Cdc45	CRISPRko	ATCAAATTGCTTATTAAACAA	GAGGA	0.6038	122	65	-	59	6	18807406
+Cdc45	CRISPRko	CCAGATCATACCCATTACCTG	GAGAG	0.5162	71	66	+	111	14	18793178
+Cdc45	CRISPRko	CCATTACCTGGAGAGGCTATC	GAGAG	0.654	140	67	+	43	14	18793189
+Cdc45	CRISPRko	GTGCTCCTGTCTCCATAGACA	AAGAA	0.7761	166	68	-	18	17	18785192
+Cdc45	CRISPRko	TGGTCACACTGAAACAGGGCC	TGGAG	0.5581	108	69	+	78	4	18810480
+Cdc45	CRISPRko	AACCACCGAAACGAGGCGGAG	GAGAA	0.5896	126	70	-	64	11	18795875
+Cdc45	CRISPRko	CCGATTTCCGCAAGGAGTTCT	ACGAG	0.6291	144	71	-	48	2	18811538
+Cdc45	CRISPRko	CTATCCGAGCTATCAGTCTCT	GGGGG	0.4647	36	72	+	158	17	18785097
+Cdc45	CRISPRko	CAGGGAGTGGGAGGCCCGAAG	GTGAG	0.8806	189	73	-	6	7	18805385
+Cdc45	CRISPRko	TGACCGTGGTGGGCATCCCCC	CAGAG	0.4971	67	74	-	129	17	18785104
+Cdc45	CRISPRko	CCATGTCTCTAATGGAGAGTC	CTGAG	0.5529	113	75	-	85	14	18793239
+Cdc45	CRISPRko	ACGGTGATGGGTCAGAGCCTT	CAGAG	0.4816	55	76	-	143	6	18807309
+Cdc45	CRISPRko	ACCTGGTTGTGGACCGTCTCG	TAGAA	0.4275	4	77	+	194	2	18811531
+Cdc45	CRISPRko	GAGATAGTGGAGCGAAACAGG	AAGAG	0.5326	103	78	-	97	7	18805420
+Cdc45	CRISPRko	GGTGATGGGTCAGAGCCTTCA	GAGAA	0.7028	170	79	-	30	6	18807307
+Cdc45	CRISPRko	AGCAGTGACACAAGAGCGTCC	AGGAA	0.5781	134	80	+	69	19	18781923
+Cdc45	CRISPRko	CAAGGATGCTGGCTTGGAAAA	CAGGG	0.7149	176	81	+	29	16	18786829
+Cdc45	CRISPRko	TTCCCCTTCAAGGAAACGTCC	ATGGA	0.4884	68	82	+	137	13	18794819
+Cdc45	CRISPRko	GAGCAGTAGAGAAAAGGGCCC	TGGGA	0.4743	53	83	+	153	15	18786958
+Cdc45	CRISPRko	TAGACTGCACCCGGATCTCCT	TCGAG	0.7915	191	84	-	16	11	18795838
+Cdc45	CRISPRko	ATTCACAACGTTCACTGGCCT	GTGGG	0.7957	193	85	+	14	5	18808768
+Cdc45	CRISPRko	TGTGCTTGCAAGATCCTCCAG	GTGAG	0.5132	92	86	-	116	3	18811308
+Cdc45	CRISPRko	AACCTCCATGAGTGAGCAGTA	GAGAA	0.5056	86	87	+	123	15	18786945
+Cdc45	CRISPRko	ATAGTGGAGCGAAACAGGAAG	AGGAG	0.6967	179	88	-	33	7	18805417
+Cdc45	CRISPRko	AAGTTCTTGCCACCCAGAAAC	CGGAA	0.5263	109	89	+	104	4	18810437
+Cdc45	CRISPRko	TGATAGCTCGGATAGAAAGAA	GTGAG	0.6241	164	90	-	50	17	18785076
+Cdc45	CRISPRko	GCATCGTCGATTCTTTGTCTA	TGGAG	0.4178	14	91	+	200	17	18785192
+Cdc45	CRISPRko	TTCATACTGCTCGTAGTCAAA	GAGGA	0.4916	80	92	+	134	8	18801834
+Cdc45	CRISPRko	CCTGTTTCGCTCCACTATCTC	CTGAA	0.4253	21	93	+	195	7	18805435
+Cdc45	CRISPRko	CTTCAGAGAAGCGCACACGGT	TAGAA	0.4285	23	94	-	193	6	18807291
+Cdc45	CRISPRko	AAGGTATTCAAAGCTCACCTG	GAGGA	0.7694	196	95	+	22	3	18811305
+Cdc45	CRISPRko	ACCGTCTCGTAGAACTCCTTG	CGGAA	0.5441	128	96	+	90	2	18811543
+Cdc45	CRISPRko	ACAATTACTTTGACCTCTCAG	GTGAG	0.8187	212	97	-	10	18	18784809
+Cdc45	CRISPRko	AAACTGCATATCTTGAACATA	AAGAG	0.4379	41	98	-	182	4	18810400
+Cdc45	CRISPRko	GCGTGGATGATGTCCAAGGAC	CTGAA	0.752	200	99	-	24	9	18798696
+Cdc45	CRISPRko	GGAAAGACATCCTCTTTGACT	ACGAG	0.512	107	100	-	117	8	18801833
+Cdc45	CRISPRko	GCTGCCCCGCTGAGCGTGGAA	CAGGG	0.4021	10	101	-	216	17	18785135
+Cdc45	CRISPRko	CGTCATAACCACCGAAACGAG	GCGGA	0.4017	11	102	-	217	11	18795881
+Cdc45	CRISPRko	CTGTGCCCTGTTCCACGCTCA	GCGGG	0.5396	136	103	+	93	17	18785140
+Cdc45	CRISPRko	TTTCTCGCAGATTCCCCTTCA	AGGAA	0.5535	146	104	+	83	13	18794808
+Cdc45	CRISPRko	TTTCAGTCCATGGACGTTTCC	TTGAA	0.4141	26	105	-	206	13	18794815
+Cdc45	CRISPRko	CAAGTGCTCACCGAGTACACA	AAGGA	0.4781	90	106	+	147	16	18786776
+Cdc45	CRISPRko	CATGGTGATGTTTGACCTGGC	GTGGA	0.6241	188	107	-	49	9	18798715
+Cdc45	CRISPRko	ACTACGAGCAGTATGAATATT	ATGGG	0.4087	30	108	-	210	8	18801815
+Cdc45	CRISPRko	TTCTATCCGAGCTATCAGTCT	CTGGG	0.3882	17	109	+	224	17	18785095
+Cdc45	CRISPRko	GTGTGTGATACCCACAGGCCA	GTGAA	0.5632	165	110	-	76	5	18808768
+Cdc45	CRISPRko	GCAAAGACGACATCACTGGCC	AGGAA	0.7328	217	111	+	27	14	18793277
+Cdc45	CRISPRko	CCCAGAGACTGATAGCTCGGA	TAGAA	0.4481	70	112	-	175	17	18785085
+Cdc45	CRISPRko	CTTCCCCTGAAGCAAGTCAAG	CAGAA	0.5276	145	113	-	101	13	18794842
+Cdc45	CRISPRko	GTCAGTCCAACAATGGCCCAC	CTGGA	0.4526	76	114	+	171	10	18797397
+Cdc45	CRISPRko	ATGTCTCTAATGGAGAGTCCT	GAGAA	0.4202	49	115	-	199	14	18793237
+Cdc45	CRISPRko	TGCTTGAACCCAAACTGAATG	CTGAA	0.4803	102	116	+	146	14	18793307
+Cdc45	CRISPRko	GCTTGGAAAACAGGGTGACAT	CTGGA	0.5694	177	117	+	72	16	18786840
+Cdc45	CRISPRko	AGGAGATCCGGGTGCAGTCTA	CAGAG	0.3816	18	118	+	231	11	18795853
+Cdc45	CRISPRko	TGTGCCCTGTTCCACGCTCAG	CGGGG	0.4596	82	119	+	168	17	18785141
+Cdc45	CRISPRko	TGTACCAACCTCGTCACTTCC	CAGGG	0.3894	31	120	-	223	15	18786966
+Cdc45	CRISPRko	AGAAAAGGGCCCTGGGAAGTG	ACGAG	0.4826	115	121	+	141	15	18786967
+Cdc45	CRISPRko	TCAGGAGATAGTGGAGCGAAA	CAGGA	0.5095	138	122	-	120	7	18805424
+Cdc45	CRISPRko	GACCGTCTCGTAGAACTCCTT	GCGGA	0.5027	131	123	+	127	2	18811542
+Cdc45	CRISPRko	TCCAGGAGTTCCTTGCAGACA	TGGGG	0.7618	236	124	-	23	12	18795102
+Cdc45	CRISPRko	CTTCTAACCGTGTGCGCTTCT	CTGAA	0.3273	8	125	+	251	6	18807300
+Cdc45	CRISPRko	CTACGAGCAGTATGAATATTA	TGGGA	0.3863	32	126	-	229	8	18801814
+Cdc45	CRISPRko	CTCTAAAGATGTCGTCGTAGG	CAGGA	0.3176	6	127	+	256	6	18807378
+Cdc45	CRISPRko	CACAGTTTATGAGGATGAAAT	AGGAG	0.5538	182	128	+	82	5	18808860
+Cdc45	CRISPRko	TCAGTCCAACAATGGCCCACC	TGGAA	0.4296	74	129	+	191	10	18797398
+Cdc45	CRISPRko	CTCATAAACTGTGGAGCCAAC	GTGGA	0.4554	97	130	-	170	5	18808837
+Cdc45	CRISPRko	TCACTGGCCAGGAATTTATGC	TTGAA	0.5084	147	131	+	121	14	18793289
+Cdc45	CRISPRko	CAGTTTATGAGGATGAAATAG	GAGAA	0.6042	210	132	+	58	5	18808862
+Cdc45	CRISPRko	TGGGAAGTGACGAGGTTGGTA	CAGAG	0.4842	133	133	+	139	15	18786979
+Cdc45	CRISPRko	AAGAACTTGAAACTGCATATC	TTGAA	0.3358	22	134	-	250	4	18810409
+Cdc45	CRISPRko	TTGTCACTATTTCAGGAGATA	GTGGA	0.5267	171	135	-	103	7	18805435
+Cdc45	CRISPRko	TCTAAAGATGTCGTCGTAGGC	AGGAA	0.4075	63	136	+	211	6	18807379
+Cdc45	CRISPRko	CACAGCATGTCATTCAGGTCC	TTGGA	0.3873	48	137	+	227	9	18798694
+Cdc45	CRISPRko	CTCTTGTGTCACTGCTGTCCT	GAGGG	0.6892	240	138	-	37	19	18781903
+Cdc45	CRISPRko	CTCACCGAGTACACAAAGGAC	TTGAG	0.4318	89	139	+	189	16	18786782
+Cdc45	CRISPRko	GTCCATGGACGTTTCCTTGAA	GGGGA	0.4315	88	140	-	190	13	18794810
+Cdc45	CRISPRko	TATACTGTCTTCATCAGGCTG	AAGGA	0.6307	231	141	+	47	5	18808810
+Cdc45	CRISPRko	GATAGTGGAGCGAAACAGGAA	GAGGA	0.5634	208	142	-	74	7	18805418
+Cdc45	CRISPRko	TCTATCCGAGCTATCAGTCTC	TGGGG	0.315	25	143	+	258	17	18785096
+Cdc45	CRISPRko	AGTCCATGGACGTTTCCTTGA	AGGGG	0.4146	79	144	-	205	13	18794811
+Cdc45	CRISPRko	CAGTCCATGGACGTTTCCTTG	AAGGG	0.4343	98	145	-	186	13	18794812
+Cdc45	CRISPRko	CGTTTCCTTGAAGGGGAATCT	GCGAG	0.5958	223	146	-	62	13	18794801
+Cdc45	CRISPRko	GCAGCGCAGGGAGTGGGAGGC	CCGAA	0.4633	123	147	-	162	7	18805391
+Cdc45	CRISPRko	TATAGCTTGTCCAGGTTACTC	CTGAA	0.5297	187	148	+	100	15	18787066
+Cdc45	CRISPRko	AGATCAAATTGCTTATTAAAC	AAGAG	0.34	39	149	-	248	6	18807408
+Cdc45	CRISPRko	TGCATCGTCGATTCTTTGTCT	ATGGA	0.3222	35	150	+	255	17	18785191
+Cdc45	CRISPRko	CTTTTCTCTACTGCTCACTCA	TGGAG	0.5317	192	151	-	98	15	18786938
+Cdc45	CRISPRko	TCCTCTTTGACTACGAGCAGT	ATGAA	0.6436	246	152	-	45	8	18801824
+Cdc45	CRISPRko	TATTCATACTGCTCGTAGTCA	AAGAG	0.4754	141	153	+	150	8	18801832
+Cdc45	CRISPRko	CACAAAGAGAAGGACCCTCTG	TAGAA	0.5148	178	154	+	113	3	18811365
+Cdc45	CRISPRko	CTCAGCGGGGCAGCCATCACC	AGGGG	0.4712	139	155	+	156	17	18785157
+Cdc45	CRISPRko	CGCCTCGTTTCGGTGGTTATG	ACGGG	0.3759	61	156	+	234	11	18795893
+Cdc45	CRISPRko	TTTCCTTGAAGGGGAATCTGC	GAGAA	0.5302	197	157	-	99	13	18794799
+Cdc45	CRISPRko	GTGTTGTACAGGCTTTCATGG	AGGGA	0.5386	202	158	+	94	12	18795191
+Cdc45	CRISPRko	AGAGTCCTGAGAAGGATGGCT	CAGGG	0.5405	206	159	-	91	14	18793224
+Cdc45	CRISPRko	GAGATCCGGGTGCAGTCTACA	GAGAG	0.5885	232	160	+	66	11	18795855
+Cdc45	CRISPRko	CTGGTGTTGTACAGGCTTTCA	TGGAG	0.4333	111	161	+	188	12	18795188
+Cdc45	CRISPRko	CAAAGTAATTGTGTAGAGTCC	GAGAG	0.5255	195	162	+	105	18	18784833
+Cdc45	CRISPRko	CTTCAAGGAAACGTCCATGGA	CTGAA	0.4112	96	163	+	208	13	18794824
+Cdc45	CRISPRko	ACGACATCTTTAGAGATGAAG	CAGAG	0.6119	248	164	-	56	6	18807357
+Cdc45	CRISPRko	AGCAGACTCAAGGATGCTGGC	TTGGA	0.4286	114	165	+	192	16	18786821
+Cdc45	CRISPRko	ATTTCTCGCAGATTCCCCTTC	AAGGA	0.472	152	166	+	154	13	18794807
+Cdc45	CRISPRko	TTCATTACTTCAGTAATTGAG	CTGAA	0.3232	54	167	-	253	19	18781958
+Cdc45	CRISPRko	CTATTTCATCCTCATAAACTG	TGGAG	0.4747	156	168	-	151	5	18808847
+Cdc45	CRISPRko	CTCCAGGAGTTCCTTGCAGAC	ATGGG	0.4166	106	169	-	203	12	18795103
+Cdc45	CRISPRko	CCTATTTCATCCTCATAAACT	GTGGA	0.4123	104	170	-	207	5	18808848
+Cdc45	CRISPRko	CTGTGCCTTTAGCTTTTTTGG	TCGGG	0.489	180	171	-	136	18	18784877
+Cdc45	CRISPRko	CAGTTTCAAGTTCTTGCCACC	CAGAA	0.4172	116	172	+	201	4	18810430
+Cdc45	CRISPRko	AAGCTCTGGTCTGTGCATGGG	CAGAA	0.4862	181	173	-	138	12	18795133
+Cdc45	CRISPRko	ACATCAGTCACGTACTTCATT	CTGGA	0.352	75	174	+	244	11	18795942
+Cdc45	CRISPRko	AGAAACCGGAACCAGCGTATA	CTGGA	0.2842	58	175	+	261	4	18810452
+Cdc45	CRISPRko	TGGAATGGGAGTCACCTGGTT	GTGGA	0.4454	143	176	+	179	2	18811518
+Cdc45	CRISPRko	GCTGAGGACCGGAGCAAGTTC	CTGGA	0.4483	149	177	-	174	19	18781931
+Cdc45	CRISPRko	GTCACACTGAAACAGGGCCTG	GAGAG	0.5469	234	178	+	89	4	18810482
+Cdc45	CRISPRko	GAGAGGCTATCGAGAGCCTGG	ATGAA	0.5652	251	179	+	73	14	18793199
+Cdc45	CRISPRko	CTGGACGTGGTCACACTGAAA	CAGGG	0.4505	151	180	+	173	4	18810473
+Cdc45	CRISPRko	TATGAGGATGAAATAGGAGAA	CTGAA	0.5633	250	181	+	75	5	18808867
+Cdc45	CRISPRko	TTAGCTTTTTTGGTCGGGCTT	TTGAG	0.3528	83	182	-	243	18	18784869
+Cdc45	CRISPRko	AATTGAGCTGAAGGCTGAGGA	CCGGA	0.5798	260	183	-	68	19	18781944
+Cdc45	CRISPRko	GTGATGGCTGCCCCGCTGAGC	GTGGA	0.4807	184	184	-	144	17	18785141
+Cdc45	CRISPRko	CGTATACTGGACGTGGTCACA	CTGAA	0.4806	183	185	+	145	4	18810467
+Cdc45	CRISPRko	GAGTCCTGAGAAGGATGGCTC	AGGGA	0.5177	220	186	-	109	14	18793223
+Cdc45	CRISPRko	AAGCTGCTCACTTCTTTCTAT	CCGAG	0.5041	205	187	+	126	17	18785080
+Cdc45	CRISPRko	AGGGGAATCTGCGAGAAATGA	TTGAA	0.5102	214	188	-	119	13	18794790
+Cdc45	CRISPRko	GAAGAGGAGGCAGCGCAGGGA	GTGGG	0.5532	249	189	-	84	7	18805400
+Cdc45	CRISPRko	CTGACCCATCACCGTCACTGT	CTGAA	0.3637	94	190	+	239	6	18807330
+Cdc45	CRISPRko	TTCCTTTCATTACTTCAGTAA	TTGAG	0.495	203	191	-	132	19	18781963
+Cdc45	CRISPRko	TGATGGCTGCCCCGCTGAGCG	TGGAA	0.4038	124	192	-	213	17	18785140
+Cdc45	CRISPRko	CTGCCCTGTTTCAGGAGTAAC	CTGGA	0.3232	84	193	-	254	15	18787065
+Cdc45	CRISPRko	TGTCACTATTTCAGGAGATAG	TGGAG	0.4432	158	194	-	180	7	18805434
+Cdc45	CRISPRko	TTGAAGAATCTGCAAATAAAT	TTGGG	0.4967	209	195	-	130	13	18794769
+Cdc45	CRISPRko	AATTTCTGCTTGACTTGCTTC	AGGGG	0.4509	167	196	+	172	13	18794849
+Cdc45	CRISPRko	ACCCATTACCTGGAGAGGCTA	TCGAG	0.4617	174	197	+	167	14	18793187
+Cdc45	CRISPRko	CGGTTTCTGGGTGGCAAGAAC	TTGAA	0.3694	105	198	-	236	4	18810424
+Cdc45	CRISPRko	GTGGCTTGGAGGTGCTTCTTG	GCGAG	0.5522	257	199	+	87	15	18787027
+Cdc45	CRISPRko	CCCTCAGGACAGCAGTGACAC	AAGAG	0.5078	226	200	+	122	19	18781913
+Cdc45	CRISPRko	CAAGTTCTTGCCACCCAGAAA	CCGGA	0.4347	163	201	+	185	4	18810436
+Cdc45	CRISPRko	TGAGCAGTAGAGAAAAGGGCC	CTGGG	0.5194	247	202	+	107	15	18786957
+Cdc45	CRISPRko	TCAGTAATTGAGCTGAAGGCT	GAGGA	0.5113	237	203	-	118	19	18781949
+Cdc45	CRISPRko	CTTCAGTAATTGAGCTGAAGG	CTGAG	0.4947	222	204	-	133	19	18781951
+Cdc45	CRISPRko	GTCAAGCAGAAATTTCAGTCC	ATGGA	0.4252	159	205	-	196	13	18794827
+Cdc45	CRISPRko	AAACAGGAAGAGGAGGCAGCG	CAGGG	0.5399	263	206	-	92	7	18805406
+Cdc45	CRISPRko	ATTTCTGCTTGACTTGCTTCA	GGGGA	0.4233	160	207	+	197	13	18794850
+Cdc45	CRISPRko	GTCAAAGTAATTGTGTAGAGT	CCGAG	0.3569	117	208	+	242	18	18784831
+Cdc45	CRISPRko	TGTATCAGCACTGGTCCCTCC	ATGAA	0.4111	150	209	-	209	12	18795195
+Cdc45	CRISPRko	CACGTCCGAGGCCACAAAGAG	AAGGA	0.462	198	210	+	165	3	18811353
+Cdc45	CRISPRko	GCCTCGTTTCGGTGGTTATGA	CGGGA	0.3694	127	211	+	237	11	18795894
+Cdc45	CRISPRko	GTTACTTACCCCATGTCTGCA	AGGAA	0.4743	213	212	+	152	12	18795104
+Cdc45	CRISPRko	AACAGGAAGAGGAGGCAGCGC	AGGGA	0.5217	261	213	-	106	7	18805405
+Cdc45	CRISPRko	TCTTTAGAGATGAAGCAGAGG	ATGAA	0.5187	259	214	-	108	6	18807351
+Cdc45	CRISPRko	GAACAGGGCACAGTGACCGTG	GTGGG	0.5053	244	215	-	124	17	18785117
+Cdc45	CRISPRko	GGCAAAGACGACATCACTGGC	CAGGA	0.3866	142	216	+	228	14	18793276
+Cdc45	CRISPRko	TCCACGTTGGCTCCACAGTTT	ATGAG	0.3918	148	217	+	222	5	18808847
+Cdc45	CRISPRko	TGTTGGATATCCTTCAGCCTG	ATGAA	0.4424	190	218	-	181	5	18808809
+Cdc45	CRISPRko	CCTTTTCTCTACTGCTCACTC	ATGGA	0.4167	172	219	-	202	15	18786939
+Cdc45	CRISPRko	CTAACCTCCATGAGTGAGCAG	TAGAG	0.447	199	220	+	177	15	18786943
+Cdc45	CRISPRko	GCTCAGCGGGGCAGCCATCAC	CAGGG	0.4363	194	221	+	183	17	18785156
+Cdc45	CRISPRko	GCGATGGTCTGTTGTGTGGCT	TGGAG	0.2988	120	222	+	259	15	18787012
+Cdc45	CRISPRko	CCTGGATGAAGTGGTCTGTCC	CTGAG	0.4627	215	223	+	164	14	18793215
+Cdc45	CRISPRko	GCTTCCTCCCTGGACAGGATG	AAGGA	0.4661	224	224	-	157	14	18793339
+Cdc45	CRISPRko	GTCTTTGCCACCATGTCTCTA	ATGGA	0.388	157	225	-	225	14	18793249
+Cdc45	CRISPRko	AGAGGAGGCAGCGCAGGGAGT	GGGAG	0.4818	241	226	-	142	7	18805398
+Cdc45	CRISPRko	TTGGCTCCACAGTTTATGAGG	ATGAA	0.3839	155	227	+	230	5	18808853
+Cdc45	CRISPRko	TCTTGAACATAAAGAGCAGGT	ATGAA	0.4712	230	228	-	155	4	18810390
+Cdc45	CRISPRko	GCTGGTGTTGTACAGGCTTTC	ATGGA	0.4338	201	229	+	187	12	18795187
+Cdc45	CRISPRko	TGACTTGCTTCAGGGGAAGCC	TGGAG	0.4357	207	230	+	184	13	18794859
+Cdc45	CRISPRko	ATCCTTCTCAGGACTCTCCAT	TAGAG	0.4631	229	231	+	163	14	18793243
+Cdc45	CRISPRko	ACTTGCTTCAGGGGAAGCCTG	GAGGG	0.4775	245	232	+	148	13	18794861
+Cdc45	CRISPRko	AGTTACTTACCCCATGTCTGC	AAGGA	0.3875	168	233	+	226	12	18795103
+Cdc45	CRISPRko	AAATATACTGTCTTCATCAGG	CTGAA	0.3734	161	234	+	235	5	18808807
+Cdc45	CRISPRko	ATTGAGCTGAAGGCTGAGGAC	CGGAG	0.484	258	235	-	140	19	18781943
+Cdc45	CRISPRko	CATCAGTCACGTACTTCATTC	TGGAA	0.4067	186	236	+	212	11	18795943
+Cdc45	CRISPRko	CGCTCTTGTGTCACTGCTGTC	CTGAG	0.3773	173	237	-	233	19	18781905
+Cdc45	CRISPRko	TTGACTTGCTTCAGGGGAAGC	CTGGA	0.3773	175	238	+	232	13	18794858
+Cdc45	CRISPRko	GGTTTAAGCTCTGGTCTGTGC	ATGGG	0.3585	169	239	-	241	12	18795138
+Cdc45	CRISPRko	AGCTTTTTTGGTCGGGCTTTT	GAGAA	0.3359	162	240	-	249	18	18784867
+Cdc45	CRISPRko	AGGGTGACATCTGGAGTGCCC	TGGAA	0.3172	154	241	+	257	16	18786851
+Cdc45	CRISPRko	GACCTGGCGTGGATGATGTCC	AAGGA	0.4463	233	242	-	178	9	18798702
+Cdc45	CRISPRko	CCCCATGTCTGCAAGGAACTC	CTGGA	0.4637	256	243	+	160	12	18795112
+Cdc45	CRISPRko	GGCAGCAGAAAGCACCAGCTC	TCGGA	0.4619	252	244	-	166	18	18784841
+Cdc45	CRISPRko	TGGACAAGCTATACCTTGGTC	TAGAG	0.364	185	245	-	238	15	18787043
+Cdc45	CRISPRko	CTGTCCCTGAGCCATCCTTCT	CAGGA	0.3981	204	246	+	219	14	18793230
+Cdc45	CRISPRko	TTATTAAACAAGAGGATGACC	TTGAG	0.4228	225	247	-	198	6	18807396
+Cdc45	CRISPRko	TGTGGAGCCAACGTGGACCTG	TTGGA	0.4634	262	248	-	161	5	18808828
+Cdc45	CRISPRko	CCCCTGGTGATGGCTGCCCCG	CTGAG	0.4035	211	249	-	214	17	18785147
+Cdc45	CRISPRko	GTCGGGCTTTTGAGAAGGCAG	CAGAA	0.4477	254	250	-	176	18	18784857
+Cdc45	CRISPRko	TTCCTTCCAGGTGGGCCATTG	TTGGA	0.4026	218	251	-	215	10	18797392
+Cdc45	CRISPRko	AAGGACTTGAGCAGATGTCTG	CTGAG	0.396	221	252	+	220	16	18786797
+Cdc45	CRISPRko	TTTCTGCTTGACTTGCTTCAG	GGGAA	0.3919	235	253	+	221	13	18794851
+Cdc45	CRISPRko	CAGACAGTGACGGTGATGGGT	CAGAG	0.3598	216	254	-	240	6	18807318
+Cdc45	CRISPRko	AAGAGGAGGCAGCGCAGGGAG	TGGGA	0.4147	255	255	-	204	7	18805399
+Cdc45	CRISPRko	CTTGCTCCCTCCAGGCTTCCC	CTGAA	0.3997	253	256	-	218	13	18794857
+Cdc45	CRISPRko	CCACAGTTTATGAGGATGAAA	TAGGA	0.3422	227	257	+	246	5	18808859
+Cdc45	CRISPRko	GCAGACTCAAGGATGCTGGCT	TGGAA	0.341	228	258	+	247	16	18786822
+Cdc45	CRISPRko	GGCGATGGTCTGTTGTGTGGC	TTGGA	0.2085	219	259	+	263	15	18787011
+Cdc45	CRISPRko	CAGGGTGACATCTGGAGTGCC	CTGGA	0.3456	242	260	+	245	16	18786850
+Cdc45	CRISPRko	AAATTTCTGCTTGACTTGCTT	CAGGG	0.3252	239	261	+	252	13	18794848
+Cdc45	CRISPRko	AGGGTCCTTCTCTTTGTGGCC	TCGGA	0.2105	238	262	-	262	3	18811347
+Cdc45	CRISPRko	TGGTTCCGGTTTCTGGGTGGC	AAGAA	0.2943	243	263	-	260	4	18810430
+Scml2	CRISPRko	CACACACCGAATCAATATTGC	GAGGG	0.8799	13	1	-	3	4	161178101
+Scml2	CRISPRko	GGAGCCAGGTTACGTCTACGA	CTGGA	0.6857	1	2	+	52	4	161178160
+Scml2	CRISPRko	TGTTCTTACTACGTGTACTAA	CTGGA	0.6956	15	3	+	46	5	161187312
+Scml2	CRISPRko	GAGATGTCCAAGTAGCCCCAG	GTGAA	0.8203	64	4	+	6	11	161215261
+Scml2	CRISPRko	GCGAATACCATGGGGCAAACA	GCGAA	0.7794	60	5	+	12	2	161171523
+Scml2	CRISPRko	GCTTTGATCAGTGCCTGTATC	TGGGG	0.761	52	6	-	24	24	161243524
+Scml2	CRISPRko	AGTTGGATGGTGTCGCCTCAC	AGGAG	0.6106	5	7	+	78	6	161192413
+Scml2	CRISPRko	GTTGCCTGAAGAGGTCGGCGA	GGGGG	0.5892	2	8	-	89	26	161255162
+Scml2	CRISPRko	TGAAAAAAGACCAAGGCGCAA	AAGAA	0.6199	18	9	+	73	7	161202033
+Scml2	CRISPRko	CAATGACCGAAGCAACACACA	CCGAA	0.7977	88	10	-	10	4	161178116
+Scml2	CRISPRko	CTGGATCGATAAGTGCTCCTT	CTGAA	0.5866	7	11	+	91	3	161177298
+Scml2	CRISPRko	TGAGGATGCAGAGACAATCGT	TTGAG	0.5914	19	12	-	86	25	161246294
+Scml2	CRISPRko	TCCTTGAAGTTTGAGCCCCTC	TAGAG	0.6247	40	13	-	68	21	161237585
+Scml2	CRISPRko	CATACTGTTCAGCTCCCTCCA	GTGAA	0.6909	62	14	+	48	24	161243404
+Scml2	CRISPRko	GGTGTGTGTTGCTTCGGTCAT	TGGAA	0.5493	9	15	+	112	4	161178129
+Scml2	CRISPRko	ATCCAACTGGGAAGATGTCTC	GGGAG	0.8123	120	16	-	8	6	161192385
+Scml2	CRISPRko	AATTTTGAATTCATTAGTTGG	TGGAG	0.7224	96	17	-	35	4	161178052
+Scml2	CRISPRko	CTCCTTGATCTGCAAAAAGTC	TGGGA	0.5857	44	18	-	92	19	161231457
+Scml2	CRISPRko	CTCCTTGATCTGCAAAAAGTC	TGGGA	0.5857	45	19	-	93	18	161230148
+Scml2	CRISPRko	CTCCTTGATCTGCAAAAAGTC	TGGGA	0.5857	46	20	-	94	17	161228734
+Scml2	CRISPRko	CTGGGAAAAAACCCAGGGGCA	GAGGA	0.7593	115	21	+	25	11	161215198
+Scml2	CRISPRko	AAACAGAAAATGGGGAACCAA	AGGAA	0.6948	94	22	+	47	25	161246269
+Scml2	CRISPRko	TCCCAGACTTTTTGCAGATCA	AGGAG	0.7145	104	23	+	37	19	161231467
+Scml2	CRISPRko	CTCCTTGATCTGCAAAAAGTC	TGGGA	0.5857	47	24	-	95	16	161227430
+Scml2	CRISPRko	TCCCAGACTTTTTGCAGATCA	AGGAG	0.7145	105	25	+	38	18	161230158
+Scml2	CRISPRko	CTCCTTGATCTGCAAAAAGTC	TGGGA	0.5857	48	26	-	96	15	161225934
+Scml2	CRISPRko	TCCCAGACTTTTTGCAGATCA	AGGAG	0.7145	107	27	+	39	16	161227440
+Scml2	CRISPRko	CTCCTTGATCTGCAAAAAGTC	TGGGA	0.5857	49	28	-	97	14	161224518
+Scml2	CRISPRko	TCCCAGACTTTTTGCAGATCA	AGGAG	0.7145	108	29	+	40	15	161225944
+Scml2	CRISPRko	CTCCTTGATCTGCAAAAAGTC	TGGGA	0.5857	50	30	-	98	13	161223214
+Scml2	CRISPRko	AATAAAGCTGAGAAAAAACGC	AAGGG	0.527	23	31	+	127	19	161231411
+Scml2	CRISPRko	CTCCTTGATCTGCAAAAAGTC	TGGGA	0.5857	51	32	-	99	12	161221723
+Scml2	CRISPRko	TCCCAGACTTTTTGCAGATCA	AGGAG	0.7145	110	33	+	41	13	161223224
+Scml2	CRISPRko	AATAAAGCTGAGAAAAAACGC	AAGGG	0.527	24	34	+	128	18	161230102
+Scml2	CRISPRko	CTTCATATCACTTTTGATGGA	TGGAG	0.6556	95	35	+	57	6	161192339
+Scml2	CRISPRko	TCCCAGACTTTTTGCAGATCA	AGGAG	0.7145	111	36	+	42	12	161221733
+Scml2	CRISPRko	AATAAAGCTGAGAAAAAACGC	AAGGG	0.527	25	37	+	129	17	161228688
+Scml2	CRISPRko	AATAAAGCTGAGAAAAAACGC	AAGGG	0.527	27	38	+	130	15	161225888
+Scml2	CRISPRko	ATTTATAAAACAGGGTACACA	CTGAA	0.6525	97	39	+	60	5	161187269
+Scml2	CRISPRko	AATAAAGCTGAGAAAAAACGC	AAGGG	0.527	28	40	+	131	14	161224472
+Scml2	CRISPRko	CCAGACCTGGGAAACATGACC	AAGGA	0.588	70	41	+	90	10	161213310
+Scml2	CRISPRko	AATAAAGCTGAGAAAAAACGC	AAGGG	0.527	30	42	+	132	12	161221677
+Scml2	CRISPRko	TTGTGGCAGGACAGATCATAA	ATGGA	0.7669	147	43	-	15	6	161192280
+Scml2	CRISPRko	AGTCTAAGACTCCACCAACTA	ATGAA	0.7552	137	44	+	26	4	161178053
+Scml2	CRISPRko	ATTCATCAGACATACAACCTG	TTGGG	0.5927	80	45	+	84	4	161178224
+Scml2	CRISPRko	GGTTGGGTGAATCCTCTGCCC	CTGGG	0.7763	154	46	-	13	11	161215199
+Scml2	CRISPRko	CGCAAAAGAAGAACCAGACTA	TGGAG	0.686	116	47	+	51	7	161202049
+Scml2	CRISPRko	GACCAGGTTGAAGGGTCCTTA	CAGAA	0.5417	54	48	-	117	26	161255085
+Scml2	CRISPRko	GAAGTTTGAGCCCCTCTAGAG	GTGGA	0.6986	127	49	-	44	21	161237580
+Scml2	CRISPRko	AGCCTGCAGTGTGATAACTTT	TTGAG	0.8025	165	50	+	9	24	161243473
+Scml2	CRISPRko	AACCACTTCGGTCCAGGCCCT	GTGAA	0.6901	128	51	+	49	23	161242077
+Scml2	CRISPRko	TCTGGGAACCTTAGATGAGAC	AGGAG	0.5317	61	52	-	123	25	161246328
+Scml2	CRISPRko	AACAAAGCTGAAAAAAAACCC	AAGGG	0.651	122	53	+	62	20	161234624
+Scml2	CRISPRko	GATGAAGATTGAAGCTGTCGA	TAGAA	0.4911	4	54	+	180	6	161192260
+Scml2	CRISPRko	AATGATGCACTATTCACTGGA	GGGAG	0.534	68	55	-	120	24	161243406
+Scml2	CRISPRko	AAGGACCCTTCAACCTGGTCT	GTGGA	0.742	161	56	+	28	26	161255101
+Scml2	CRISPRko	AATAAAGCTGAGAAAAAACGC	AAGGG	0.5087	26	57	+	164	16	161227384
+Scml2	CRISPRko	AATAAAGCTGAGAAAAAACGC	AAGGG	0.5087	29	58	+	165	13	161223168
+Scml2	CRISPRko	CTTCATCCACAGACCAGGTTG	AAGGG	0.8441	192	59	-	4	26	161255096
+Scml2	CRISPRko	AAACAGTCACCACACCTTGGA	CTGAG	0.5265	71	60	+	134	19	161231439
+Scml2	CRISPRko	AAACAGTCACCACACCTTGGA	CTGAG	0.5265	72	61	+	135	18	161230130
+Scml2	CRISPRko	AAACAGTCACCACACCTTGGA	CTGAG	0.5265	73	62	+	136	17	161228716
+Scml2	CRISPRko	AAACAGTCACCACACCTTGGA	CTGAG	0.5265	74	63	+	137	16	161227412
+Scml2	CRISPRko	GTAGTAAGAACATGGGCCATG	ATGAA	0.5274	85	64	-	126	5	161187288
+Scml2	CRISPRko	GGAGATTTACTTCAGCCTCCA	CTGGG	0.8826	209	65	+	2	4	161178265
+Scml2	CRISPRko	CAAATGATGCACTATTCACTG	GAGGG	0.5185	59	66	-	153	24	161243408
+Scml2	CRISPRko	AAACAGTCACCACACCTTGGA	CTGAG	0.5265	75	67	+	138	15	161225916
+Scml2	CRISPRko	GGAAATACTTCATCCCCTAAG	AAGGG	0.4814	22	68	+	192	21	161237695
+Scml2	CRISPRko	AAACAGTCACCACACCTTGGA	CTGAG	0.5265	76	69	+	139	14	161224500
+Scml2	CRISPRko	AAACAGTCACCACACCTTGGA	CTGAG	0.5265	77	70	+	140	13	161223196
+Scml2	CRISPRko	ATACCATGGGGCAAACAGCGA	ATGAA	0.4639	10	71	+	207	2	161171527
+Scml2	CRISPRko	AGAAGCCTTGTTTCAGGACAC	TGGGG	0.792	208	72	-	11	26	161255063
+Scml2	CRISPRko	AAACAGTCACCACACCTTGGA	CTGAG	0.5265	78	73	+	141	12	161221705
+Scml2	CRISPRko	TGTTGGGACGTGTGAACAAGG	AGGAG	0.664	166	74	+	56	4	161178243
+Scml2	CRISPRko	GTAGGAACTTGAGCCTCACGT	CTGAA	0.5926	138	75	-	85	24	161243499
+Scml2	CRISPRko	CAGACCTGGGAAACATGACCA	AGGAG	0.6554	169	76	+	58	10	161213311
+Scml2	CRISPRko	GCAGAGGATTCACCCAACCTT	TGGAA	0.4845	43	77	+	188	11	161215216
+Scml2	CRISPRko	TTGCACAACACTTGTTTTCCG	TGGAG	0.4421	8	78	-	226	21	161237614
+Scml2	CRISPRko	CAGACTTTCTGGGAACCTTAG	ATGAG	0.652	175	79	-	61	25	161246335
+Scml2	CRISPRko	TGTTGCCTGAAGAGGTCGGCG	AGGGG	0.4876	57	80	-	182	26	161255163
+Scml2	CRISPRko	AGATACGTTACATGTTGCCTG	AAGAG	0.5645	131	81	-	109	26	161255175
+Scml2	CRISPRko	TTAATAGTAATGCCCTTCTTA	GGGGA	0.6196	171	82	-	74	21	161237697
+Scml2	CRISPRko	GTCGGCGAGGGGGCCTGATAT	TTGAG	0.516	87	83	-	159	26	161255149
+Scml2	CRISPRko	CGAATCAATATTGCGAGGGTC	ACGGG	0.434	3	84	-	243	4	161178094
+Scml2	CRISPRko	GCTTCCAATTTCATACCAATT	TTGAA	0.5902	158	85	-	88	4	161178069
+Scml2	CRISPRko	GTTAGTACACGTAGTAAGAAC	ATGGG	0.4649	42	86	-	206	5	161187298
+Scml2	CRISPRko	TCCCAGACTTTTTGCAGATCA	AGGAG	0.5226	106	87	+	147	17	161228744
+Scml2	CRISPRko	TTGACTGGGACAAGTACTTGA	AAGAG	0.6314	190	88	+	65	3	161177271
+Scml2	CRISPRko	TCCCAGACTTTTTGCAGATCA	AGGAG	0.5226	109	89	+	148	14	161224528
+Scml2	CRISPRko	ACAGTCACCACACCTTGGACT	GAGGA	0.7626	244	90	+	16	19	161231441
+Scml2	CRISPRko	AAACAGAACTTTGAAAGATTG	CCGAG	0.6358	198	91	+	63	8	161209259
+Scml2	CRISPRko	ACAGTCACCACACCTTGGACT	GAGGA	0.7626	245	92	+	17	18	161230132
+Scml2	CRISPRko	TCCCAACAGGTTGTATGTCTG	ATGAA	0.5576	152	93	-	110	4	161178215
+Scml2	CRISPRko	ACAGTCACCACACCTTGGACT	GAGGA	0.7626	246	94	+	18	17	161228718
+Scml2	CRISPRko	ACAGTCACCACACCTTGGACT	GAGGA	0.7626	247	95	+	19	16	161227414
+Scml2	CRISPRko	GCCCCGCAGAAGTCTTATTAG	ATGAA	0.4371	31	96	-	237	22	161239823
+Scml2	CRISPRko	ACAGTCACCACACCTTGGACT	GAGGA	0.7626	248	97	+	20	15	161225918
+Scml2	CRISPRko	CAGAACTTTGAAAGATTGCCA	AGGAG	0.6289	202	98	+	66	9	161209676
+Scml2	CRISPRko	GGTTTGATGAATCCTCTGCCC	TTGGG	0.7295	235	99	-	34	10	161213260
+Scml2	CRISPRko	ACAGTCACCACACCTTGGACT	GAGGA	0.7626	249	100	+	21	14	161224502
+Scml2	CRISPRko	GATTTGGTGACTCTTTTGCCC	TTGGG	0.7337	238	101	-	33	20	161234632
+Scml2	CRISPRko	ACAGTCACCACACCTTGGACT	GAGGA	0.7626	250	102	+	22	13	161223198
+Scml2	CRISPRko	TAGACGCTTGCCCAGACTTTC	TGGGA	0.4525	56	103	-	217	25	161246347
+Scml2	CRISPRko	ACAGTCACCACACCTTGGACT	GAGGA	0.7626	251	104	+	23	12	161221707
+Scml2	CRISPRko	AACCTGTTGGGACGTGTGAAC	AAGGA	0.4224	16	105	+	262	4	161178239
+Scml2	CRISPRko	TATTTATATACACACAAACTT	AAGAG	0.5101	117	106	-	163	23	161241988
+Scml2	CRISPRko	ATCAACTTCATATCACTTTTG	ATGGA	0.5903	193	107	+	87	6	161192334
+Scml2	CRISPRko	TAGGTAGCTGCTGAACCTTCT	GTGGA	0.7481	254	108	-	27	23	161242042
+Scml2	CRISPRko	ACATACAACCTGTTGGGACGT	GTGAA	0.4202	14	109	+	267	4	161178233
+Scml2	CRISPRko	AAGAATGATTTTTGGAGACTT	GTGGA	0.6254	220	110	+	67	4	161178199
+Scml2	CRISPRko	AAAAAGACCAAGGCGCAAAAG	AAGAA	0.6681	233	111	+	55	7	161202036
+Scml2	CRISPRko	AGGTTGGAAAAAACCCAAGGG	CAGAG	0.5961	207	112	+	83	10	161213257
+Scml2	CRISPRko	CATGGAGATTGTGGCCCCTTC	CTGGA	0.6795	240	113	+	53	23	161242026
+Scml2	CRISPRko	TGAAGATGTAGAAGAAATAAC	TGGAA	0.6214	226	114	-	71	22	161239768
+Scml2	CRISPRko	ACTGATCAAAGCAAACCAGGT	AAGAA	0.4588	90	115	+	210	24	161243548
+Scml2	CRISPRko	TGCTTCGGTCATTGGAATTAC	TGGAG	0.4336	55	116	+	245	4	161178138
+Scml2	CRISPRko	AGCTGGGAAAAAACCCAGGGG	CAGAG	0.6133	224	117	+	77	11	161215196
+Scml2	CRISPRko	CGGCGAGGGGGCCTGATATTT	GAGGA	0.5049	135	118	-	167	26	161255147
+Scml2	CRISPRko	AAAAGATACGTTACATGTTGC	CTGAA	0.4069	12	119	-	291	26	161255178
+Scml2	CRISPRko	GCTCCTTGATCTGCAAAAAGT	CTGGG	0.4156	34	120	-	272	17	161228735
+Scml2	CRISPRko	AAAAAAGCTGGGAAAAAACCC	AGGGG	0.5664	200	121	+	107	11	161215191
+Scml2	CRISPRko	CATTTTGACTGGGACAAGTAC	TTGAA	0.5157	148	122	+	160	3	161177267
+Scml2	CRISPRko	TGGGACCAGTGTACTTACTTT	TGGAG	0.526	167	123	+	142	8	161209229
+Scml2	CRISPRko	TCTAGAGGGGCTCAAACTTCA	AGGAA	0.5067	144	124	+	166	21	161237596
+Scml2	CRISPRko	GCTCCTTGATCTGCAAAAAGT	CTGGG	0.4156	37	125	-	273	14	161224519
+Scml2	CRISPRko	GAAAATGGGGAACCAAAGGAA	AAGAG	0.7409	282	126	+	29	25	161246274
+Scml2	CRISPRko	TTGTCCTACCAAAGAAAAAGG	GTGGA	0.4842	121	127	+	190	21	161237735
+Scml2	CRISPRko	AACAAAGGTTGGAAAAAACCC	AAGGG	0.5664	203	128	+	108	10	161213252
+Scml2	CRISPRko	AAAGAAAAAGGGTGGAAAGTC	TGGAA	0.4569	100	129	+	212	21	161237745
+Scml2	CRISPRko	TCTCCTTGATCTGCAAAAAGT	CTGGG	0.4084	32	130	-	283	19	161231458
+Scml2	CRISPRko	TCTCCTTGATCTGCAAAAAGT	CTGGG	0.4084	33	131	-	284	18	161230149
+Scml2	CRISPRko	CAGGAGATGTATTACAGCCAC	CAGGA	0.4271	63	132	+	255	6	161192433
+Scml2	CRISPRko	TGAAAAAAAACCCAAGGGCAA	AAGAG	0.9328	319	133	+	1	20	161234632
+Scml2	CRISPRko	TCTCCTTGATCTGCAAAAAGT	CTGGG	0.4084	35	134	-	285	16	161227431
+Scml2	CRISPRko	CATTTAGAGGCATTAGGGCAG	CAGGA	0.44	91	135	-	230	26	161255214
+Scml2	CRISPRko	ACAGTATGAACTTTCCCATCA	AAGAA	0.4302	69	136	-	252	24	161243376
+Scml2	CRISPRko	TCTCCTTGATCTGCAAAAAGT	CTGGG	0.4084	36	137	-	286	15	161225935
+Scml2	CRISPRko	GGTGGCTGTAATACATCTCCT	GTGAG	0.5814	221	138	-	101	6	161192419
+Scml2	CRISPRko	GTTGGAAAAAACCCAAGGGCA	GAGGA	0.8189	316	139	+	7	10	161213259
+Scml2	CRISPRko	TCTTAAAACAGATAATAGAGG	AGGAG	0.5832	225	140	+	100	23	161242172
+Scml2	CRISPRko	TCTCCTTGATCTGCAAAAAGT	CTGGG	0.4084	38	141	-	287	13	161223215
+Scml2	CRISPRko	GATTCACCCAACCTTTGGAAG	ATGAA	0.5199	176	142	+	149	11	161215222
+Scml2	CRISPRko	TCTCCTTGATCTGCAAAAAGT	CTGGG	0.4084	39	143	-	288	12	161221724
+Scml2	CRISPRko	CAAAAAAGCTGGGAAAAAACC	CAGGG	0.5743	223	144	+	104	11	161215190
+Scml2	CRISPRko	CTGTACTGCTCACACTAAGAG	CTGAA	0.4953	150	145	-	178	21	161237495
+Scml2	CRISPRko	GGGCAGAGGATTCATCAAACC	TGGAA	0.3999	17	146	+	313	10	161213275
+Scml2	CRISPRko	CGACTGGATGGTAGTGACAAT	AAGAA	0.7037	289	147	+	43	4	161178178
+Scml2	CRISPRko	GGAAAAGATGAAACCAGACCT	GGGAA	0.6357	269	148	+	64	10	161213297
+Scml2	CRISPRko	TGAGTAGCCAGCCTTTCAGAC	GTGAG	0.5235	189	149	+	145	24	161243495
+Scml2	CRISPRko	AAATAGAAACACATCCTTGGC	CTGAA	0.4287	81	150	-	253	23	161242137
+Scml2	CRISPRko	TCTGGCTTCATCTTCCAAAGG	TTGGG	0.7381	303	151	-	31	11	161215218
+Scml2	CRISPRko	TGCTTTGATCAGTGCCTGTAT	CTGGG	0.4171	65	152	-	270	24	161243525
+Scml2	CRISPRko	CGGTGTGTGTTGCTTCGGTCA	TTGGA	0.4058	41	153	+	295	4	161178128
+Scml2	CRISPRko	ACAGGCCTGCACAGTCCGCTG	GAGAA	0.7682	323	154	-	14	23	161242098
+Scml2	CRISPRko	TGGAAAAGATGAAACCAGACC	TGGGA	0.5281	213	155	+	125	10	161213296
+Scml2	CRISPRko	GCTCACACTAAGAGCTGAAAA	AAGAG	0.5329	219	156	-	121	21	161237488
+Scml2	CRISPRko	GCTTCATCTTCCAAAGGTTGG	GTGAA	0.6982	296	157	-	45	11	161215214
+Scml2	CRISPRko	GTGGATGAAGTGATACAGTTT	ATGAA	0.4255	84	158	+	258	26	161255122
+Scml2	CRISPRko	CAGAACTTTGAAAGATTGCCG	AGGAG	0.5481	230	159	+	113	8	161209262
+Scml2	CRISPRko	AGATGAGACAGGAGCAGAACG	ATGAG	0.579	243	160	-	102	25	161246316
+Scml2	CRISPRko	AAAATTGTCCTACCAAAGAAA	AAGGG	0.844	342	161	+	5	21	161237731
+Scml2	CRISPRko	TTATGATCTGTCCTGCCACAA	TTGGA	0.4583	136	162	+	211	6	161192295
+Scml2	CRISPRko	AAGGCCTGCACATTGGTCATT	TAGAG	0.4957	173	163	-	175	26	161255231
+Scml2	CRISPRko	TCACTATTTTAGATGGCCCCG	CAGAA	0.4439	124	164	-	225	22	161239838
+Scml2	CRISPRko	ACCTGTTGGGACGTGTGAACA	AGGAG	0.4752	155	165	+	196	4	161178240
+Scml2	CRISPRko	AAAACAGAAAATGGGGAACCA	AAGGA	0.6051	273	166	+	80	25	161246268
+Scml2	CRISPRko	TCAAACTTCAAGGAAGTCTCC	ACGGA	0.4872	170	167	+	183	21	161237607
+Scml2	CRISPRko	CAAACTTCAAGGAAGTCTCCA	CGGAA	0.5167	199	168	+	157	21	161237608
+Scml2	CRISPRko	ACTGGAAATACTTCATCCCCT	AAGAA	0.4412	129	169	+	229	21	161237692
+Scml2	CRISPRko	TACTACGTGTACTAACTGGAT	CTGAA	0.376	21	170	+	337	5	161187318
+Scml2	CRISPRko	GAAAACAAAATCCACCTCTAG	AGGGG	0.5748	256	171	+	103	21	161237580
+Scml2	CRISPRko	TCTTAGTGTGAGCAGTACAGA	GAGAA	0.5168	204	172	+	155	21	161237511
+Scml2	CRISPRko	AAGACTTTGGTTGTTCAGAGG	AAGAA	0.4253	102	173	-	259	21	161237534
+Scml2	CRISPRko	ACAGAACTTTGAAAGATTGCC	GAGGA	0.4663	157	174	+	205	8	161209261
+Scml2	CRISPRko	AACAACCAAAGTCTTCTACCT	CTGGG	0.4702	163	175	+	200	21	161237555
+Scml2	CRISPRko	GGAAAACAAAATCCACCTCTA	GAGGG	0.4862	178	176	+	186	21	161237579
+Scml2	CRISPRko	AACACACACCGAATCAATATT	GCGAG	0.4376	132	177	-	234	4	161178103
+Scml2	CRISPRko	TGTGAGGCGACACCATCCAAC	TGGGA	0.4343	125	178	-	242	6	161192399
+Scml2	CRISPRko	AAATGGGGAACCAAAGGAAAA	GAGAA	0.6895	318	179	+	50	25	161246276
+Scml2	CRISPRko	CTTCATCTAATAAGACTTCTG	CGGGG	0.4098	89	180	+	281	22	161239832
+Scml2	CRISPRko	TGGGAAAACAAAATCCACCTC	TAGAG	0.4194	101	181	+	269	21	161237577
+Scml2	CRISPRko	TTGCTTCGGTCATTGGAATTA	CTGGA	0.4339	126	182	+	244	4	161178137
+Scml2	CRISPRko	ATTTAGAGGCATTAGGGCAGC	AGGAA	0.468	168	183	-	204	26	161255213
+Scml2	CRISPRko	CTGTTGGGACGTGTGAACAAG	GAGGA	0.4448	149	184	+	223	4	161178242
+Scml2	CRISPRko	TAAAAACTGGGCTTGGTCTTT	CCGAG	0.5014	205	185	-	169	20	161234657
+Scml2	CRISPRko	AGGGCAGAGGATTCATCAAAC	CTGGA	0.3521	20	186	+	359	10	161213274
+Scml2	CRISPRko	ATGTTGCCTGAAGAGGTCGGC	GAGGG	0.5192	232	187	-	150	26	161255164
+Scml2	CRISPRko	ATCAGTGCCTGTATCTGGGGT	AGGAA	0.413	103	188	-	279	24	161243518
+Scml2	CRISPRko	TTCTTAAAACAGATAATAGAG	GAGGA	0.4209	118	189	+	264	23	161242171
+Scml2	CRISPRko	TTTTAATAGTAATGCCCTTCT	TAGGG	0.3893	58	190	-	325	21	161237699
+Scml2	CRISPRko	CATCATTTGCACTTCGCTTTC	TTGAA	0.3168	11	191	+	373	24	161243435
+Scml2	CRISPRko	CTGGAAAAGATGAAACCAGAC	CTGGG	0.5321	262	192	+	122	10	161213295
+Scml2	CRISPRko	GATCCAGTTAGTACACGTAGT	AAGAA	0.269	6	193	-	383	5	161187304
+Scml2	CRISPRko	ACATGTTGCCTGAAGAGGTCG	GCGAG	0.6087	312	194	-	79	26	161255166
+Scml2	CRISPRko	TTCTGGGAACCTTAGATGAGA	CAGGA	0.5467	277	195	-	114	25	161246329
+Scml2	CRISPRko	GTGCAAATGATGCACTATTCA	CTGGA	0.4313	145	196	-	248	24	161243411
+Scml2	CRISPRko	GGAAGATGAAGCCAGACCAGG	AAGAG	0.6735	340	197	+	54	11	161215238
+Scml2	CRISPRko	GGCATTAGGGCAGCAGGAAAA	CCGGA	0.7146	360	198	-	36	26	161255206
+Scml2	CRISPRko	ATCCCAACTATGAAATTATTT	TGGAG	0.6183	321	199	-	75	6	161192227
+Scml2	CRISPRko	GGGACAAGTACTTGAAAGAGA	CTGGA	0.7402	367	200	+	30	3	161177277
+Scml2	CRISPRko	TTATTGGTGTGACTATGACTC	CCGAG	0.6226	335	201	+	69	6	161192377
+Scml2	CRISPRko	TGCAAATGATGCACTATTCAC	TGGAG	0.3584	53	202	-	352	24	161243410
+Scml2	CRISPRko	GTCAATTGTGCCTTTCAGGCC	AAGGA	0.6525	350	203	+	59	23	161242134
+Scml2	CRISPRko	CTGCCACAATTGGAGCTGTCT	GTGGA	0.3861	82	204	+	328	6	161192307
+Scml2	CRISPRko	TGCTTCTTCATCAGCTAAAAA	CTGGG	0.4729	214	205	-	197	20	161234672
+Scml2	CRISPRko	AAGCCCAGTTTTTAGCTGATG	AAGAA	0.4195	146	206	+	268	20	161234679
+Scml2	CRISPRko	ACTCCCGAGACATCTTCCCAG	TTGGA	0.615	338	207	+	76	6	161192394
+Scml2	CRISPRko	CACTCCAAAATAATTTCATAG	TTGGG	0.4849	227	208	+	187	6	161192235
+Scml2	CRISPRko	AGACGCTTGCCCAGACTTTCT	GGGAA	0.4303	164	209	-	251	25	161246346
+Scml2	CRISPRko	CTGTGAGGCGACACCATCCAA	CTGGG	0.4144	139	210	-	276	6	161192400
+Scml2	CRISPRko	GGTCAGGTACAGTTATATAAC	TTGAA	0.3606	67	211	-	349	26	161255039
+Scml2	CRISPRko	ATGAGACAGGAGCAGAACGAT	GAGGA	0.7377	384	212	-	32	25	161246314
+Scml2	CRISPRko	ACAGAACTTTGAAAGATTGCC	AAGGA	0.4245	156	213	+	260	9	161209675
+Scml2	CRISPRko	GCGCAAAAGAAGAACCAGACT	ATGGA	0.5231	270	214	+	146	7	161202048
+Scml2	CRISPRko	CCGTGGAGACTTCCTTGAAGT	TTGAG	0.5184	263	215	-	154	21	161237596
+Scml2	CRISPRko	TAGAAGACTTTGGTTGTTCAG	AGGAA	0.4449	197	216	-	222	21	161237537
+Scml2	CRISPRko	CAGTTGGATGGTGTCGCCTCA	CAGGA	0.4067	130	217	+	292	6	161192412
+Scml2	CRISPRko	ACTCCATCCATCAAAAGTGAT	ATGAA	0.3495	66	218	-	361	6	161192330
+Scml2	CRISPRko	ACATTGGTCATTTAGAGGCAT	TAGGG	0.3716	86	219	-	342	26	161255222
+Scml2	CRISPRko	TCTTCATCTAATAAGACTTCT	GCGGG	0.4421	201	220	+	227	22	161239831
+Scml2	CRISPRko	ATGACATTCACAGGGCCTGGA	CCGAA	0.6217	359	221	-	70	23	161242073
+Scml2	CRISPRko	TATCACTTTTGATGGATGGAG	TGGAG	0.382	98	222	+	331	6	161192344
+Scml2	CRISPRko	CAGAAGCCTTGTTTCAGGACA	CTGGG	0.4553	217	223	-	213	26	161255064
+Scml2	CRISPRko	ATATCACTTTTGATGGATGGA	GTGGA	0.3978	114	224	+	317	6	161192343
+Scml2	CRISPRko	TGGGACCAGTGCATTTACTTT	TGGAG	0.5004	261	225	+	171	9	161209643
+Scml2	CRISPRko	TATGATCTGTCCTGCCACAAT	TGGAG	0.427	177	226	+	256	6	161192296
+Scml2	CRISPRko	GTCCTTACAGAAGCCTTGTTT	CAGGA	0.3952	112	227	-	322	26	161255071
+Scml2	CRISPRko	AAATGATGCACTATTCACTGG	AGGGA	0.4696	234	228	-	201	24	161243407
+Scml2	CRISPRko	TTACTTCAGCCTCCACTGGGT	AAGGA	0.5981	355	229	+	81	4	161178271
+Scml2	CRISPRko	ATTTCTTAAAACAGATAATAG	AGGAG	0.512	276	230	+	161	23	161242169
+Scml2	CRISPRko	CTGCTGAACCTTCTGTGGATC	CAGGA	0.3482	79	231	-	362	23	161242035
+Scml2	CRISPRko	GCTCTTAGTGTGAGCAGTACA	GAGAG	0.4306	191	232	+	250	21	161237509
+Scml2	CRISPRko	TGCCACAATTGGAGCTGTCTG	TGGAG	0.4948	264	233	+	179	6	161192308
+Scml2	CRISPRko	TTGTTTTCCGTGGAGACTTCC	TTGAA	0.4692	242	234	-	202	21	161237603
+Scml2	CRISPRko	GTGAGGCGACACCATCCAACT	GGGAA	0.5167	286	235	-	158	6	161192398
+Scml2	CRISPRko	TCCGCTGGAGAATGACATTCA	CAGGG	0.5187	294	236	-	151	23	161242084
+Scml2	CRISPRko	GAACCTTCTGTGGATCCAGGA	AGGGG	0.5731	341	237	-	105	23	161242030
+Scml2	CRISPRko	CAGCTCTTAGTGTGAGCAGTA	CAGAG	0.4794	253	238	+	193	21	161237507
+Scml2	CRISPRko	TTTGGAAGATGAAGCCAGACC	AGGAA	0.5964	365	239	+	82	11	161215235
+Scml2	CRISPRko	ACTCCAAAATAATTTCATAGT	TGGGA	0.4506	228	240	+	219	6	161192236
+Scml2	CRISPRko	ACCAAGCCCAGTTTTTAGCTG	ATGAA	0.4272	194	241	+	254	20	161234676
+Scml2	CRISPRko	TACCTGTCTGAAATATTCAGA	AGGAG	0.5004	278	242	-	170	3	161177303
+Scml2	CRISPRko	GTTTCATCTTTTCCAGGTTTG	ATGAA	0.6203	377	243	-	72	10	161213275
+Scml2	CRISPRko	ACTTCATATCACTTTTGATGG	ATGGA	0.4105	174	244	+	280	6	161192338
+Scml2	CRISPRko	ACCAAAGACCAGCAAGAAAGC	AGGAA	0.49	274	245	+	181	21	161237655
+Scml2	CRISPRko	TTGTGTGTATATAAATAAACA	TGGAG	0.4993	284	246	+	172	23	161242007
+Scml2	CRISPRko	TACCAAAGACCAGCAAGAAAG	CAGGA	0.4974	283	247	+	174	21	161237654
+Scml2	CRISPRko	TTGGCAGAAATTTTCAAGAAA	GCGAA	0.5439	345	248	-	116	24	161243437
+Scml2	CRISPRko	ACTGTACCTGACCCCAGTGTC	CTGAA	0.3061	83	249	+	380	26	161255062
+Scml2	CRISPRko	AGGTAGAAGACTTTGGTTGTT	CAGAG	0.318	93	250	-	372	21	161237540
+Scml2	CRISPRko	GGCAGAGGATTCACCCAACCT	TTGGA	0.4418	237	251	+	228	11	161215215
+Scml2	CRISPRko	AAGCAGGAAAATCTAAGTCTA	CTGGA	0.3156	92	252	+	374	21	161237672
+Scml2	CRISPRko	AATTCAAAATTGGTATGAAAT	TGGAA	0.3397	99	253	+	367	4	161178077
+Scml2	CRISPRko	TTCATCAGACATACAACCTGT	TGGGA	0.3865	143	254	+	327	4	161178225
+Scml2	CRISPRko	TCAGCTTCTTTAAGTACACTG	ATGAA	0.4031	162	255	+	311	22	161239801
+Scml2	CRISPRko	AACTTTTCATCAGTGTACTTA	AAGAA	0.4636	266	256	-	208	22	161239796
+Scml2	CRISPRko	GCCTGTATCTGGGGTAGGAAC	TTGAG	0.4954	299	257	-	177	24	161243512
+Scml2	CRISPRko	ACTATTAAAATTGTCCTACCA	AAGAA	0.3977	159	258	+	318	21	161237725
+Scml2	CRISPRko	AGCAGGAAAATCTAAGTCTAC	TGGAA	0.4205	211	259	+	266	21	161237673
+Scml2	CRISPRko	CAACCAGTACCAAAGACCAGC	AAGAA	0.526	334	260	+	143	21	161237647
+Scml2	CRISPRko	CTGCTCTTTTGGGAAATGAAG	AAGAG	0.5688	372	261	+	106	7	161202083
+Scml2	CRISPRko	CAGGCTTTGGCAGAAATTTTC	AAGAA	0.4363	241	262	-	238	24	161243443
+Scml2	CRISPRko	AGGAGATGTATTACAGCCACC	AGGAA	0.3892	153	263	+	326	6	161192434
+Scml2	CRISPRko	TGGCTCCAGTAATTCCAATGA	CCGAA	0.445	258	264	-	221	4	161178131
+Scml2	CRISPRko	ACAACCAAAGTCTTCTACCTC	TGGGA	0.3611	134	265	+	348	21	161237556
+Scml2	CRISPRko	GGGCAAAACAGTCACCACACC	TTGGA	0.4033	179	266	+	303	19	161231434
+Scml2	CRISPRko	GTCCTGAAACAAGGCTTCTGT	AAGGA	0.4981	310	267	+	173	26	161255080
+Scml2	CRISPRko	GGGCAAAACAGTCACCACACC	TTGGA	0.4033	180	268	+	304	18	161230125
+Scml2	CRISPRko	TTAGACGCTTGCCCAGACTTT	CTGGG	0.3671	142	269	-	344	25	161246348
+Scml2	CRISPRko	CTCTAGAGGGGCTCAAACTTC	AAGGA	0.3788	151	270	+	335	21	161237595
+Scml2	CRISPRko	GGGCAAAACAGTCACCACACC	TTGGA	0.4033	181	271	+	305	17	161228711
+Scml2	CRISPRko	CCATCCAACTGGGAAGATGTC	TCGGG	0.4398	255	272	-	231	6	161192387
+Scml2	CRISPRko	CCCTGTGAATGTCATTCTCCA	GCGGA	0.5238	343	273	+	144	23	161242094
+Scml2	CRISPRko	TTAAAACAGATAATAGAGGAG	GAGAA	0.5299	364	274	+	124	23	161242174
+Scml2	CRISPRko	GGGCAAAACAGTCACCACACC	TTGGA	0.4033	182	275	+	306	16	161227407
+Scml2	CRISPRko	GGGCAAAACAGTCACCACACC	TTGGA	0.4033	183	276	+	307	15	161225911
+Scml2	CRISPRko	TGAACCTTCTGTGGATCCAGG	AAGGG	0.4728	293	277	-	198	23	161242031
+Scml2	CRISPRko	TCTCTCTGTACTGCTCACACT	AAGAG	0.4769	298	278	-	194	21	161237500
+Scml2	CRISPRko	GGGCAAAACAGTCACCACACC	TTGGA	0.4033	184	279	+	308	14	161224495
+Scml2	CRISPRko	AGGAGCAGAACGATGAGGATG	CAGAG	0.5447	378	280	-	115	25	161246307
+Scml2	CRISPRko	ACTTGTCCCAGTCAAAATGAT	CTGGA	0.3806	160	281	-	333	3	161177250
+Scml2	CRISPRko	GGGCAAAACAGTCACCACACC	TTGGA	0.4033	185	282	+	309	13	161223191
+Scml2	CRISPRko	TGTCCTACCAAAGAAAAAGGG	TGGAA	0.4546	280	283	+	216	21	161237736
+Scml2	CRISPRko	GGGCAAAACAGTCACCACACC	TTGGA	0.4033	186	284	+	310	12	161221700
+Scml2	CRISPRko	GAATTCAAAATTGGTATGAAA	TTGGA	0.2688	113	285	+	384	4	161178076
+Scml2	CRISPRko	TCTATTATCTGTTTTAAGAAA	TAGAA	0.313	123	286	-	375	23	161242155
+Scml2	CRISPRko	TCCAAGAAGCTGCAGAAGAAC	CTGGG	0.54	380	287	+	118	9	161209621
+Scml2	CRISPRko	GGGCAAAAGAGTCACCAAATC	TCGGA	0.4024	187	288	+	312	20	161234647
+Scml2	CRISPRko	TCCAAGAAGCTGCAGAAGAAC	CTGGG	0.54	381	289	+	119	8	161209207
+Scml2	CRISPRko	TAGTAATGCCCTTCTTAGGGG	ATGAA	0.3844	172	290	-	329	21	161237693
+Scml2	CRISPRko	AAGAACCAGACTATGGAGACT	TAGAA	0.4634	292	291	+	209	7	161202057
+Scml2	CRISPRko	GATTCATCAAACCTGGAAAAG	ATGAA	0.5552	391	292	+	111	10	161213283
+Scml2	CRISPRko	GGAGAATGACATTCACAGGGC	CTGGA	0.5049	336	293	-	168	23	161242078
+Scml2	CRISPRko	ACACAGGCCTGCACAGTCCGC	TGGAG	0.4151	231	294	-	274	23	161242100
+Scml2	CRISPRko	CTTGTCCCAGTCAAAATGATC	TGGAA	0.3421	140	295	-	365	3	161177249
+Scml2	CRISPRko	CAACCAAAGTCTTCTACCTCT	GGGAA	0.4309	257	296	+	249	21	161237557
+Scml2	CRISPRko	CAACTATGAAATTATTTTGGA	GTGGA	0.2481	119	297	-	388	6	161192223
+Scml2	CRISPRko	TCCTGTCTCATCTAAGGTTCC	CAGAA	0.3273	141	298	+	369	25	161246339
+Scml2	CRISPRko	GTAGAAGACTTTGGTTGTTCA	GAGGA	0.398	195	299	-	315	21	161237538
+Scml2	CRISPRko	GTTGCACAACACTTGTTTTCC	GTGGA	0.3932	188	300	-	323	21	161237615
+Scml2	CRISPRko	TTTATCCTTACCCAGTGGAGG	CTGAA	0.451	295	301	-	218	4	161178265
+Scml2	CRISPRko	GACACAGGCCTGCACAGTCCG	CTGGA	0.3056	133	302	-	381	23	161242101
+Scml2	CRISPRko	ACTTTCCCATCAAAGAAGGCT	ATGAA	0.5117	356	303	-	162	24	161243367
+Scml2	CRISPRko	CTTTGGAAGATGAAGCCAGAC	CAGGA	0.5186	366	304	+	152	11	161215234
+Scml2	CRISPRko	AGTGACAATAAGAATGATTTT	TGGAG	0.469	315	305	+	203	4	161178190
+Scml2	CRISPRko	CTGAAGATGTAGAAGAAATAA	CTGGA	0.5265	388	306	-	133	22	161239769
+Scml2	CRISPRko	TGGCACCTGCAGTGTTCTTTA	AGGAG	0.4504	301	307	+	220	5	161187345
+Scml2	CRISPRko	ACCGAAGTGGTTAGGTAGCTG	CTGAA	0.4216	259	308	-	263	23	161242053
+Scml2	CRISPRko	ATTTCATAGTTGGGATGAAGA	TTGAA	0.3978	206	309	+	316	6	161192247
+Scml2	CRISPRko	ACTTACTTTTGGAGATGAAAA	CAGAA	0.4956	347	310	+	176	8	161209241
+Scml2	CRISPRko	GAACCTTAGATGAGACAGGAG	CAGAA	0.5167	370	311	-	156	25	161246323
+Scml2	CRISPRko	TCACTTCATCCACAGACCAGG	TTGAA	0.4208	272	312	-	265	26	161255099
+Scml2	CRISPRko	TGCACTATTCACTGGAGGGAG	CTGAA	0.3917	215	313	-	324	24	161243401
+Scml2	CRISPRko	TCTATTTCTTAAAACAGATAA	TAGAG	0.4822	348	314	+	191	23	161242166
+Scml2	CRISPRko	TATACTTCCAAGAAGCTGCAG	AAGAA	0.4374	304	315	+	235	9	161209615
+Scml2	CRISPRko	TATTTTTCCAGATCATTTTGA	CTGGG	0.4768	344	316	+	195	3	161177254
+Scml2	CRISPRko	GGCAAAAGAGTCACCAAATCT	CGGAA	0.435	300	317	+	241	20	161234648
+Scml2	CRISPRko	TATACTTCCAAGAAGCTGCAG	AAGAA	0.4374	305	318	+	236	8	161209201
+Scml2	CRISPRko	GAACTGCTCTTTTGGGAAATG	AAGAA	0.4394	309	319	+	232	7	161202080
+Scml2	CRISPRko	GAGACTTAGAACTGCTCTTTT	GGGAA	0.4381	308	320	+	233	7	161202072
+Scml2	CRISPRko	ATCCCAGACTTTTTGCAGATC	AAGGA	0.4548	328	321	+	214	17	161228743
+Scml2	CRISPRko	TGCTGAACCTTCTGTGGATCC	AGGAA	0.3633	196	322	-	347	23	161242034
+Scml2	CRISPRko	AAAACATTCACCTGGGGCTAC	TTGGA	0.406	252	323	-	293	11	161215257
+Scml2	CRISPRko	ATCCCAGACTTTTTGCAGATC	AAGGA	0.4548	331	324	+	215	14	161224527
+Scml2	CRISPRko	TTTGGAGATGAAAACAGAACT	TTGAA	0.4868	362	325	+	184	9	161209662
+Scml2	CRISPRko	TTTGGAGATGAAAACAGAACT	TTGAA	0.4868	363	326	+	185	8	161209248
+Scml2	CRISPRko	GAACTGTTTATCCTTACCCAG	TGGAG	0.4242	287	327	-	261	4	161178271
+Scml2	CRISPRko	CACTGGAGGGAGCTGAACAGT	ATGAA	0.4093	271	328	-	282	24	161243392
+Scml2	CRISPRko	TAGTGACAATAAGAATGATTT	TTGGA	0.4145	279	329	+	275	4	161178189
+Scml2	CRISPRko	CTGGCACCTGCAGTGTTCTTT	AAGGA	0.3636	212	330	+	346	5	161187344
+Scml2	CRISPRko	CATCCAACTGGGAAGATGTCT	CGGGA	0.3732	218	331	-	341	6	161192386
+Scml2	CRISPRko	TTAAAGAAGCTGAAGATGTAG	AAGAA	0.4843	379	332	-	189	22	161239778
+Scml2	CRISPRko	GGAGACTTAGAACTGCTCTTT	TGGGA	0.3578	216	333	+	353	7	161202071
+Scml2	CRISPRko	CACAGACAGCTCCAATTGTGG	CAGGA	0.3788	236	334	-	336	6	161192295
+Scml2	CRISPRko	TACTTAAAGAAGCTGAAGATG	TAGAA	0.471	383	335	-	199	22	161239781
+Scml2	CRISPRko	CTGGGACCAGTGCATTTACTT	TTGGA	0.3703	239	336	+	343	9	161209642
+Scml2	CRISPRko	ATTTACTTTTGGAGATGAAAA	CAGAA	0.435	346	337	+	240	9	161209655
+Scml2	CRISPRko	ACTAATGAATTCAAAATTGGT	ATGAA	0.3088	210	338	+	379	4	161178070
+Scml2	CRISPRko	TCATCAGTGTACTTAAAGAAG	CTGAA	0.3335	222	339	-	368	22	161239790
+Scml2	CRISPRko	GGCCTCTTCTTCATTTCCCAA	AAGAG	0.4142	313	340	-	277	7	161202076
+Scml2	CRISPRko	CCAGTGCATTTACTTTTGGAG	ATGAA	0.4083	302	341	+	289	9	161209648
+Scml2	CRISPRko	CCAGTGTACTTACTTTTGGAG	ATGAA	0.4445	376	342	+	224	8	161209234
+Scml2	CRISPRko	CATCTAAGGTTCCCAGAAAGT	CTGGG	0.3746	267	343	+	339	25	161246347
+Scml2	CRISPRko	TAGAGGTGGATTTTGTTTTCC	CAGAG	0.4362	371	344	-	239	21	161237564
+Scml2	CRISPRko	TGGAGACTTAGAACTGCTCTT	TTGGG	0.4164	339	345	+	271	7	161202070
+Scml2	CRISPRko	CTACTTCATAGCCTTCTTTGA	TGGGA	0.3534	260	346	+	356	24	161243373
+Scml2	CRISPRko	CAAAATAATTTCATAGTTGGG	ATGAA	0.2527	229	347	+	387	6	161192240
+Scml2	CRISPRko	GCTTCAATCTTCATCCCAACT	ATGAA	0.406	325	348	-	294	6	161192239
+Scml2	CRISPRko	CCAAGAAGCTGCAGAAGAACC	TGGGA	0.4327	374	349	+	246	9	161209622
+Scml2	CRISPRko	CCAAGAAGCTGCAGAAGAACC	TGGGA	0.4327	375	350	+	247	8	161209208
+Scml2	CRISPRko	ATCCCAGACTTTTTGCAGATC	AAGGA	0.4046	326	351	+	297	19	161231466
+Scml2	CRISPRko	ATCCCAGACTTTTTGCAGATC	AAGGA	0.4046	327	352	+	298	18	161230157
+Scml2	CRISPRko	ATCCCAGACTTTTTGCAGATC	AAGGA	0.4046	329	353	+	299	16	161227439
+Scml2	CRISPRko	CTGGGACCAGTGTACTTACTT	TTGGA	0.3757	290	354	+	338	8	161209228
+Scml2	CRISPRko	AGAGAATATTGGCATACCTGT	CTGAA	0.3738	288	355	-	340	3	161177317
+Scml2	CRISPRko	CCGCAGAAGTCTTATTAGATG	AAGAA	0.4138	352	356	-	278	22	161239820
+Scml2	CRISPRko	ATCCCAGACTTTTTGCAGATC	AAGGA	0.4046	330	357	+	300	15	161225943
+Scml2	CRISPRko	TTTAATAGTAATGCCCTTCTT	AGGGG	0.3418	265	358	-	366	21	161237698
+Scml2	CRISPRko	TATTTCTTAAAACAGATAATA	GAGGA	0.3527	275	359	+	358	23	161242168
+Scml2	CRISPRko	ATCCCAGACTTTTTGCAGATC	AAGGA	0.4046	332	360	+	301	13	161223223
+Scml2	CRISPRko	ATCCCAGACTTTTTGCAGATC	AAGGA	0.4046	333	361	+	302	12	161221732
+Scml2	CRISPRko	TTAGAACTGCTCTTTTGGGAA	ATGAA	0.4258	385	362	+	257	7	161202077
+Scml2	CRISPRko	TTTAATTAAAAAACAGAAAAT	GGGGA	0.3955	322	363	+	321	25	161246259
+Scml2	CRISPRko	TTAATTAAAAAACAGAAAATG	GGGAA	0.4052	349	364	+	296	25	161246260
+Scml2	CRISPRko	TTATCACACTGCAGGCTTTGG	CAGAA	0.4069	357	365	-	290	24	161243454
+Scml2	CRISPRko	CCTACTTCATAGCCTTCTTTG	ATGGG	0.3993	337	366	+	314	24	161243372
+Scml2	CRISPRko	CAAAGAAAAAGGGTGGAAAGT	CTGGA	0.3809	320	367	+	332	21	161237744
+Scml2	CRISPRko	TGTGGCCCCTTCCTGGATCCA	CAGAA	0.3432	291	368	+	363	23	161242035
+Scml2	CRISPRko	AGAACTGTTTATCCTTACCCA	GTGGA	0.3832	324	369	-	330	4	161178272
+Scml2	CRISPRko	GGCATACCTGTCTGAAATATT	CAGAA	0.2584	268	370	-	386	3	161177307
+Scml2	CRISPRko	CATCCCAACTATGAAATTATT	TTGGA	0.3233	285	371	-	370	6	161192228
+Scml2	CRISPRko	TTTTATACTTCCAAGAAGCTG	CAGAA	0.3588	306	372	+	350	9	161209612
+Scml2	CRISPRko	TTTTATACTTCCAAGAAGCTG	CAGAA	0.3588	307	373	+	351	8	161209198
+Scml2	CRISPRko	CAATTTTGAATTCATTAGTTG	GTGGA	0.3113	281	374	-	377	4	161178053
+Scml2	CRISPRko	ATTTTTCCAGATCATTTTGAC	TGGGA	0.2283	297	375	+	390	3	161177255
+Scml2	CRISPRko	GATCAGTGCCTGTATCTGGGG	TAGGA	0.3969	369	376	-	320	24	161243519
+Scml2	CRISPRko	CTTTCCACCCTTTTTCTTTGG	TAGGA	0.3103	314	377	-	378	21	161237728
+Scml2	CRISPRko	TGCAGTGTTCTTTAAGGAGGT	AAGAA	0.3794	361	378	+	334	5	161187352
+Scml2	CRISPRko	TCCTCCTCTATTATCTGTTTT	AAGAA	0.2449	311	379	-	389	23	161242161
+Scml2	CRISPRko	AATGCTTTTCTTACCTCCTTA	AAGAA	0.3549	351	380	-	354	5	161187349
+Scml2	CRISPRko	TTTGTGTGTATATAAATAAAC	ATGGA	0.219	317	381	+	391	23	161242006
+Scml2	CRISPRko	GCTCTTTTTTCAGCTCTTAGT	GTGAG	0.3976	389	382	+	319	21	161237497
+Scml2	CRISPRko	ACCCTTCAACCTGGTCTGTGG	ATGAA	0.3529	353	383	+	357	26	161255105
+Scml2	CRISPRko	ATACCTGTCTGAAATATTCAG	AAGGA	0.352	354	384	-	360	3	161177304
+Scml2	CRISPRko	TGGATTTTGTTTTCCCAGAGG	TAGAA	0.3541	368	385	-	355	21	161237558
+Scml2	CRISPRko	CCAGGTTCTTCTGCAGCTTCT	TGGAA	0.3648	382	386	-	345	9	161209610
+Scml2	CRISPRko	TACTTCATAGCCTTCTTTGAT	GGGAA	0.313	358	387	+	376	24	161243374
+Scml2	CRISPRko	TTTTTAATTAAAAAACAGAAA	ATGGG	0.3428	386	388	+	364	25	161246257
+Scml2	CRISPRko	AGAGAACACCACCTTCTTCCT	CTGAA	0.2987	373	389	+	382	21	161237531
+Scml2	CRISPRko	CCCAGGTTCTTCTGCAGCTTC	TTGGA	0.3222	387	390	-	371	9	161209611
+Scml2	CRISPRko	TTTTAATTAAAAAACAGAAAA	TGGGG	0.2621	390	391	+	385	25	161246258
+Apoh	CRISPRko	GAAAATGGAATTGTACGCTAC	ACGAG	0.8441	1	1	+	5	3	108397346
+Apoh	CRISPRko	AGCACGGTGGCTTTCTTAACG	GGGAG	0.8153	5	2	-	8	7	108412014
+Apoh	CRISPRko	AAGGAAACTGGACCCGATTGC	CAGAA	0.7741	6	3	+	14	5	108407465
+Apoh	CRISPRko	ATGTTGCTATTGCAGGACGGA	GTGAG	0.8641	20	4	+	2	1	108395405
+Apoh	CRISPRko	GCTGAAGGCCTATAATCCTTA	AGGAG	0.9188	28	5	-	1	5	108407337
+Apoh	CRISPRko	AGCCAGAGTATGTCCTTTCGC	TGGAA	0.6673	7	6	+	28	3	108397315
+Apoh	CRISPRko	GAATTGTACGCTACACGAGTT	TTGAA	0.6473	3	7	+	33	3	108397353
+Apoh	CRISPRko	CCAAGACTCACGAGCACAAGC	AGGAA	0.7385	23	8	-	18	4	108404881
+Apoh	CRISPRko	ATCGAGATTCCCTCGTGCTTC	AAGGG	0.8167	36	9	+	7	7	108412186
+Apoh	CRISPRko	AGGAGTAGACAATCTGCTCCC	CAGGG	0.8638	47	10	-	3	2	108395885
+Apoh	CRISPRko	GCGAGCACCGGGGAAACCATT	GCGGA	0.7284	34	11	-	20	1	108395346
+Apoh	CRISPRko	GCAAAGCCGGTGCTTCTATAT	AAGGA	0.6436	24	12	+	34	6	108409202
+Apoh	CRISPRko	CCTTCCCTCCGAGGCCAGAAA	ATGGG	0.7897	51	13	+	9	6	108409161
+Apoh	CRISPRko	GAAGAAGCGGAATGTACCAAG	ACGGG	0.5573	16	14	+	50	6	108409274
+Apoh	CRISPRko	ACGGTTTACCTGTCCTCTCAC	AGGAA	0.7319	48	15	+	19	2	108395955
+Apoh	CRISPRko	CACCGTGCTGTACCAAGGGAT	GAGGG	0.8511	64	16	+	4	7	108412041
+Apoh	CRISPRko	GCCAGGCTACGTGTCCAGGGG	AGGGA	0.7014	46	17	+	22	2	108395925
+Apoh	CRISPRko	TTAAAGACATCCTACGACCCT	GGGGA	0.5334	10	18	+	58	2	108395878
+Apoh	CRISPRko	TGGACCCGATTGCCAGAATGC	CTGGG	0.7614	55	19	+	16	5	108407473
+Apoh	CRISPRko	GTTCCAAAGTTTGCACTCCTT	AAGGA	0.595	32	20	+	39	5	108407332
+Apoh	CRISPRko	GTTTTGCTTGTAACCCTGGGT	AAGGA	0.6642	49	21	+	31	3	108397395
+Apoh	CRISPRko	TATATAGAAGCACCGGCTTTG	CAGGA	0.6987	63	22	-	23	6	108409188
+Apoh	CRISPRko	TTGTGTTCAGTCTGTCCGAAG	CCGGA	0.6752	60	23	+	26	2	108395824
+Apoh	CRISPRko	TGCATCAGAACTGACACCGTG	CTGAA	0.4936	15	24	+	76	8	108414289
+Apoh	CRISPRko	CTACACTGTGGAGGCTCATTG	CAGAG	0.6874	69	25	+	24	7	108412152
+Apoh	CRISPRko	AGGCGGAGAACAAGGCGAGCA	CCGGG	0.6738	67	26	-	27	1	108395360
+Apoh	CRISPRko	AGACCACTGTGTCCTGATACA	AAGAG	0.7879	85	27	-	11	5	108407372
+Apoh	CRISPRko	CTCATCTAAGTGCACGGAGGA	AGGAA	0.5115	31	28	+	68	4	108404850
+Apoh	CRISPRko	CAGGGTCGTAGGATGTCTTTA	AGGGG	0.4898	22	29	-	78	2	108395864
+Apoh	CRISPRko	AATCCTTAAGGAGTGCAAACT	TTGGA	0.4825	21	30	-	81	5	108407324
+Apoh	CRISPRko	TAAAGACATCCTACGACCCTG	GGGAG	0.4765	17	31	+	86	2	108395879
+Apoh	CRISPRko	AGAAGCGGAATGTACCAAGAC	GGGAA	0.5753	57	32	+	47	6	108409276
+Apoh	CRISPRko	GAGTCTTGCAAACTCCCCGTT	AAGAA	0.4491	4	33	+	107	7	108412012
+Apoh	CRISPRko	TTCAGACTTACGGACACATCT	CAGGG	0.7891	103	34	-	10	2	108395986
+Apoh	CRISPRko	TTGGTCCTTCTTGCCGACCTG	TAGAG	0.4497	8	35	+	106	6	108409303
+Apoh	CRISPRko	AGCCTTACCCTTGAAGCACGA	GGGAA	0.762	100	36	-	15	7	108412183
+Apoh	CRISPRko	GGAATGTGGCCCATCAACACC	CTGAG	0.5705	66	37	+	49	2	108395977
+Apoh	CRISPRko	ACCAGCTCATCTAAGTGCACG	GAGGA	0.4515	12	38	+	105	4	108404845
+Apoh	CRISPRko	TACACGAGTTTTGAATATCCC	AAGAA	0.4469	9	39	+	108	3	108397364
+Apoh	CRISPRko	TTTCGCTGGAATCTTAGAAAA	TGGAA	0.6647	87	40	+	30	3	108397330
+Apoh	CRISPRko	TCTTGGTACATTCCGCTTCTT	CTGGG	0.4575	18	41	-	100	6	108409260
+Apoh	CRISPRko	AGGGTCGTAGGATGTCTTTAA	GGGGA	0.4668	27	42	-	93	2	108395863
+Apoh	CRISPRko	CCAGCTCATCTAAGTGCACGG	AGGAA	0.5047	53	43	+	70	4	108404846
+Apoh	CRISPRko	AAGCTGGACGGCCCAGAAGAA	GCGGA	0.6271	88	44	+	36	6	108409259
+Apoh	CRISPRko	CTTAAAGACATCCTACGACCC	TGGGG	0.4423	14	45	+	111	2	108395877
+Apoh	CRISPRko	GGCGGAGAACAAGGCGAGCAC	CGGGG	0.538	68	46	-	57	1	108395359
+Apoh	CRISPRko	CCGAGGCCAGAAAATGGGTAT	GTGAA	0.8246	120	47	+	6	6	108409169
+Apoh	CRISPRko	TAAGGATTATAGGCCTTCAGC	TGGGA	0.4703	37	48	+	89	5	108407352
+Apoh	CRISPRko	AAGAAGCGGAATGTACCAAGA	CGGGA	0.549	77	49	+	52	6	108409275
+Apoh	CRISPRko	CCTTAAAGACATCCTACGACC	CTGGG	0.4235	2	50	+	129	2	108395876
+Apoh	CRISPRko	CATCCCATTCTTAAACTGTTC	CTGGA	0.5898	89	51	-	44	7	108412063
+Apoh	CRISPRko	AGCCAGGCTACGTGTCCAGGG	GAGGG	0.5448	80	52	+	54	2	108395924
+Apoh	CRISPRko	TGCCATGTTGCTATTGCAGGA	CGGAG	0.6817	113	53	+	25	1	108395401
+Apoh	CRISPRko	GACGGTTTACCTGTCCTCTCA	CAGGA	0.4579	42	54	+	99	2	108395954
+Apoh	CRISPRko	GCGGAGAACAAGGCGAGCACC	GGGGA	0.5023	70	55	-	71	1	108395358
+Apoh	CRISPRko	GCTTGCCACACTTTGCCATGA	TCGGA	0.4391	29	56	+	113	5	108407414
+Apoh	CRISPRko	CTCATTGCAGAGATGGCACTA	TCGAG	0.6585	111	57	+	32	7	108412166
+Apoh	CRISPRko	AGACATACAAGCTGGACGGCC	CAGAA	0.4753	58	58	+	87	6	108409251
+Apoh	CRISPRko	GTTGATGGGCCACATTCCTGT	GAGAG	0.591	106	59	-	42	2	108395959
+Apoh	CRISPRko	CAGCCAGAGTATGTCCTTTCG	CTGGA	0.4058	11	60	+	138	3	108397314
+Apoh	CRISPRko	GGCTACGTGTCCAGGGGAGGG	ATGAG	0.5478	96	61	+	53	2	108395929
+Apoh	CRISPRko	TTCCTTCCTCCGTGCACTTAG	ATGAG	0.4638	56	62	-	95	4	108404840
+Apoh	CRISPRko	CTTACCCAGGCATTCTGGCAA	TCGGG	0.7546	137	63	-	17	5	108407467
+Apoh	CRISPRko	CAGATATTCCTGCTTGTGCTC	GTGAG	0.5163	90	64	+	64	4	108404885
+Apoh	CRISPRko	TGGACACGTAGCCTGGCTTGC	AGGAG	0.7149	134	65	-	21	2	108395906
+Apoh	CRISPRko	CAAATGGTAGGTCATCCGGCT	TCGGA	0.3991	13	66	-	142	2	108395828
+Apoh	CRISPRko	AGATCCAGGAACAGTTTAAGA	ATGGG	0.5916	117	67	+	41	7	108412070
+Apoh	CRISPRko	CATGTGCACAGAACAAGGAAA	CTGGA	0.5244	98	68	+	60	5	108407451
+Apoh	CRISPRko	TGCAAGCCAGGCTACGTGTCC	AGGGG	0.3997	19	69	+	141	2	108395920
+Apoh	CRISPRko	GCAGCCTTACCCTTGAAGCAC	GAGGG	0.4288	40	70	-	121	7	108412185
+Apoh	CRISPRko	AAGGATTATAGGCCTTCAGCT	GGGAA	0.4304	41	71	+	120	5	108407353
+Apoh	CRISPRko	AGAAAGCCACCGTGCTGTACC	AAGGG	0.4551	59	72	+	103	7	108412034
+Apoh	CRISPRko	CATACAAGCTGGACGGCCCAG	AAGAA	0.6107	124	73	+	38	6	108409254
+Apoh	CRISPRko	CCAGGGTCGTAGGATGTCTTT	AAGGG	0.4215	30	74	-	132	2	108395865
+Apoh	CRISPRko	TACAGCACGGTGGCTTTCTTA	ACGGG	0.4882	84	75	-	79	7	108412017
+Apoh	CRISPRko	CACAGTCATGTGCACAGAACA	AGGAA	0.5926	123	76	+	40	5	108407445
+Apoh	CRISPRko	GATCCAGGAACAGTTTAAGAA	TGGGA	0.5828	119	77	+	45	7	108412071
+Apoh	CRISPRko	GAAAGCCACCGTGCTGTACCA	AGGGA	0.6128	128	78	+	37	7	108412035
+Apoh	CRISPRko	GTGAAGATCCAGGAACAGTTT	AAGAA	0.7808	155	79	+	12	7	108412066
+Apoh	CRISPRko	AGCTGGACGGCCCAGAAGAAG	CGGAA	0.7748	154	80	+	13	6	108409260
+Apoh	CRISPRko	ACAGCACGGTGGCTTTCTTAA	CGGGG	0.5191	105	81	-	63	7	108412016
+Apoh	CRISPRko	ATAAAGCCACATTTGGTTGCC	ATGAG	0.4608	71	82	+	97	6	108409227
+Apoh	CRISPRko	GCCACCGTGCTGTACCAAGGG	ATGAG	0.4596	72	83	+	98	7	108412039
+Apoh	CRISPRko	CACGAGCACAAGCAGGAATAT	CTGGG	0.5013	97	84	-	73	4	108404873
+Apoh	CRISPRko	ACATCAGTTTTGCTTGTAACC	CTGGG	0.4274	45	85	+	125	3	108397389
+Apoh	CRISPRko	GCAAGCCAGGCTACGTGTCCA	GGGGA	0.3943	26	86	+	145	2	108395921
+Apoh	CRISPRko	CTTTCGCTGGAATCTTAGAAA	ATGGA	0.4685	81	87	+	91	3	108397329
+Apoh	CRISPRko	CATTTTCTAAGATTCCAGCGA	AAGGA	0.3798	25	88	-	149	3	108397317
+Apoh	CRISPRko	GCTCATCTAAGTGCACGGAGG	AAGGA	0.4372	61	89	+	114	4	108404849
+Apoh	CRISPRko	CGGAGAACAAGGCGAGCACCG	GGGAA	0.4974	101	90	-	75	1	108395357
+Apoh	CRISPRko	CTTTCTGGAAAACGGATGCAT	CAGAA	0.4119	43	91	+	136	8	108414273
+Apoh	CRISPRko	AAGTGCACGGAGGAAGGAAAA	TGGAG	0.6665	151	92	+	29	4	108404857
+Apoh	CRISPRko	CATACCCATTTTCTGGCCTCG	GAGGG	0.4041	44	93	-	139	6	108409155
+Apoh	CRISPRko	TACAAGCAAAACTGATGTTCT	TGGGA	0.5236	122	94	-	61	3	108397371
+Apoh	CRISPRko	CCAGTACCTCTACAGGTCGGC	AAGAA	0.4772	99	95	-	85	6	108409300
+Apoh	CRISPRko	CTGGTCCCATTCAGAAAAAAC	CTGGA	0.4275	62	96	-	123	4	108404814
+Apoh	CRISPRko	GTGCTGTACCAAGGGATGAGG	GTGAA	0.5114	118	97	+	69	7	108412045
+Apoh	CRISPRko	AAATGTGGCTTTATCCTTATA	TAGAA	0.3653	39	98	-	154	6	108409205
+Apoh	CRISPRko	CTTGCCACACTTTGCCATGAT	CGGAA	0.3639	38	99	+	155	5	108407415
+Apoh	CRISPRko	AGTTCTGATGCATCCGTTTTC	CAGAA	0.3558	33	100	-	161	8	108414265
+Apoh	CRISPRko	GTACCTCTACAGGTCGGCAAG	AAGGA	0.4674	102	101	-	92	6	108409297
+Apoh	CRISPRko	TTACAAGCAAAACTGATGTTC	TTGGG	0.4372	79	102	-	115	3	108397372
+Apoh	CRISPRko	CAGCCTTACCCTTGAAGCACG	AGGGA	0.4225	65	103	-	130	7	108412184
+Apoh	CRISPRko	GTGTTGATGGGCCACATTCCT	GTGAG	0.5214	133	104	-	62	2	108395961
+Apoh	CRISPRko	GGTTGCCATGAGACATACAAG	CTGGA	0.3933	50	105	+	146	6	108409241
+Apoh	CRISPRko	ATAGCAACATGGCAGAGGAAG	GCGGA	0.5022	125	106	-	72	1	108395379
+Apoh	CRISPRko	CCTGCAATAGCAACATGGCAG	AGGAA	0.4774	114	107	-	84	1	108395385
+Apoh	CRISPRko	ACAATCTGCTCCCCAGGGTCG	TAGGA	0.5768	153	108	-	46	2	108395877
+Apoh	CRISPRko	AGCTGAAGGCCTATAATCCTT	AAGGA	0.3419	35	109	-	165	5	108407338
+Apoh	CRISPRko	AGTTCTCTGGCTTTCTGGAAA	ACGGA	0.6428	166	110	+	35	8	108414263
+Apoh	CRISPRko	TGGTCCCATTCAGAAAAAACC	TGGAA	0.445	94	111	-	109	4	108404813
+Apoh	CRISPRko	CTGCAAGCCAGGCTACGTGTC	CAGGG	0.3756	54	112	+	151	2	108395919
+Apoh	CRISPRko	CAGAGGAAGGCGGAGAACAAG	GCGAG	0.5901	163	113	-	43	1	108395367
+Apoh	CRISPRko	TAAGTGCACGGAGGAAGGAAA	ATGGA	0.5151	143	114	+	65	4	108404856
+Apoh	CRISPRko	CGTCCTGCAATAGCAACATGG	CAGAG	0.5324	149	115	-	59	1	108395388
+Apoh	CRISPRko	AGAAGAAGTGCAGCTACACTG	TGGAG	0.5706	161	116	+	48	7	108412139
+Apoh	CRISPRko	GGACCAGCTCATCTAAGTGCA	CGGAG	0.4078	73	117	+	137	4	108404843
+Apoh	CRISPRko	GAAGTAAAATGTCCCTTCCCT	CCGAG	0.4632	115	118	+	96	6	108409148
+Apoh	CRISPRko	CCCAAGACTCACGAGCACAAG	CAGGA	0.4874	131	119	-	80	4	108404882
+Apoh	CRISPRko	AAAGCCAGAGAACTGTGCTCT	GTGGG	0.5393	156	120	-	56	8	108414241
+Apoh	CRISPRko	TTGCAGCCTTACCCTTGAAGC	ACGAG	0.4447	104	121	-	110	7	108412187
+Apoh	CRISPRko	TACCCATTTTCTGGCCTCGGA	GGGAA	0.4326	95	122	-	119	6	108409153
+Apoh	CRISPRko	CATCCGTTTTCCAGAAAGCCA	GAGAA	0.5432	160	123	-	55	8	108414255
+Apoh	CRISPRko	TTCTACTGCAAAAACAAAGAG	AAGAA	0.4982	141	124	+	74	7	108412120
+Apoh	CRISPRko	CAAGGGATGAGGGTGAAGATC	CAGGA	0.4906	138	125	+	77	7	108412054
+Apoh	CRISPRko	TTTTTCTTTTCCAGGTTTTTT	CTGAA	0.5551	164	126	+	51	4	108404815
+Apoh	CRISPRko	TGCATCCGTTTTCCAGAAAGC	CAGAG	0.4532	112	127	-	104	8	108414257
+Apoh	CRISPRko	TAACGGGGAGTTTGCAAGACT	CTGAA	0.4145	82	128	-	135	7	108411998
+Apoh	CRISPRko	CAAGCCAGGCTACGTGTCCAG	GGGAG	0.4701	127	129	+	90	2	108395922
+Apoh	CRISPRko	TCACATACCCATTTTCTGGCC	TCGGA	0.3198	52	130	-	168	6	108409158

resources/KO_SP_human.txt

@@ -0,0 +1,1001 @@
+Target Gene Symbol	CRISPR Mechanism	sgRNA Sequence	PAM Sequence	On-Target Efficacy Score	Off-Target Rank	Combined Rank	Strand of sgRNA	On-Target Rank	Exon Number	sgRNA Cut Position (1-based)
+TSPAN6	CRISPRko	TATTTGTTATGGCACGAGAG	AGG	1.077	1	1	+	5	7	100630835
+TSPAN6	CRISPRko	GGTGCCCAAAAGAATAATGA	CGG	1.068	7	2	+	6	2	100635623
+TSPAN6	CRISPRko	ACCAGTAGCAATGAGCACGA	AGG	1.205	17	3	+	2	2	100635647
+TSPAN6	CRISPRko	TAGTCATGGCGTCCCCGTCT	CGG	-0.1104	2	4	-	27	1	100636683
+TSPAN6	CRISPRko	CAGTAGCAATGAGCACGAAG	GGG	1.101	26	5	+	4	2	100635649
+TSPAN6	CRISPRko	AGTCTGCAGTCTCCGAGACG	GGG	0.8323	24	6	+	9	1	100636682
+TSPAN6	CRISPRko	AATGAGCACGAAGGGGACAT	TGG	0.4856	18	7	+	16	2	100635656
+TSPAN6	CRISPRko	TGGGTTACACTATCTCATAC	TGG	-0.4735	3	8	+	33	7	100630811
+TSPAN6	CRISPRko	CCAGTAGCAATGAGCACGAA	GGG	-0.03374	15	9	+	25	2	100635648
+TSPAN6	CRISPRko	TGAATTCCCACATAGATCAC	TGG	0.6391	28	10	-	13	2	100635745
+TSPAN6	CRISPRko	TCTGAAAACAAATCCTACGA	TGG	0.697	30	11	+	12	3	100635201
+TSPAN6	CRISPRko	ACCATTATAGAGTCAGAAAT	GGG	-0.6104	10	12	-	35	6	100632531
+TSPAN6	CRISPRko	GTGTCACCGATTATAGAGAT	TGG	-0.394	14	13	-	31	5	100633510
+TSPAN6	CRISPRko	TTAGCATCCATGCAGAAGCT	CGG	1.067	40	14	+	7	2	100635580
+TSPAN6	CRISPRko	TTCTGCATGGATGCTAAAAC	TGG	-0.46	16	15	-	32	2	100635563
+TSPAN6	CRISPRko	GCAGGTAGCAAAACAACCAA	AGG	1.123	45	16	+	3	2	100635602
+TSPAN6	CRISPRko	TTGGAAGCAAGCAACTCCAA	AGG	0.3437	31	17	+	18	6	100632502
+TSPAN6	CRISPRko	TAGTCTGCAGTCTCCGAGAC	GGG	-0.07463	23	18	+	26	1	100636681
+TSPAN6	CRISPRko	TATGGCACGAGAGAGGCAGT	AGG	1.825	49	19	+	1	7	100630842
+TSPAN6	CRISPRko	ACAAAGTAAACAATGAAGTA	AGG	-0.2058	22	20	-	28	5	100633405
+TSPAN6	CRISPRko	CATCCATGCAGAAGCTCGGC	AGG	0.4986	36	21	+	15	2	100635584
+TSPAN6	CRISPRko	CCCAAATGCCAACTGCAAGA	AGG	0.2984	32	22	+	19	2	100635727
+TSPAN6	CRISPRko	TTAGTCTGCAGTCTCCGAGA	CGG	0.7125	41	23	+	11	1	100636680
+TSPAN6	CRISPRko	TGGTACCGTCATTATTCTTT	TGG	-1.031	8	24	-	45	2	100635617
+TSPAN6	CRISPRko	TCAGAAATGGGAGTCGTTGC	AGG	-0.2165	25	25	-	29	6	100632519
+TSPAN6	CRISPRko	CCCTTCGTGCTCATTGCTAC	TGG	-0.5425	20	26	-	34	2	100635637
+TSPAN6	CRISPRko	GAGCACGAAGGGGACATTGG	TGG	0.7868	44	27	+	10	2	100635659
+TSPAN6	CRISPRko	TTGAAGCAGTATAACTCTAC	AGG	-0.8396	13	28	-	42	4	100633977
+TSPAN6	CRISPRko	ATCTGTCCAATCTCTATAAT	CGG	-1.488	6	29	+	50	5	100633515
+TSPAN6	CRISPRko	CCTTCTTGCAGTTGGCATTT	GGG	-1.258	9	30	-	47	2	100635716
+TSPAN6	CRISPRko	TCCCATTTCTGACTCTATAA	TGG	-0.7623	19	31	+	38	6	100632541
+TSPAN6	CRISPRko	GGTTACCACTTACCGTATTT	TGG	-1.64	5	32	+	52	4	100633935
+TSPAN6	CRISPRko	AGTAGACAAGATCCAAAATA	CGG	0.02655	34	33	-	24	4	100633936
+TSPAN6	CRISPRko	TTTGGATCTTGTCTACTGCA	TGG	0.087	35	34	+	23	4	100633953
+TSPAN6	CRISPRko	TCTCGTTTTTTTGGTCGAAC	TGG	-1.68	4	35	-	54	3	100635219
+TSPAN6	CRISPRko	CTCTTGAAACAAGTAATGAC	TGG	0.1616	37	36	+	21	1	100636656
+TSPAN6	CRISPRko	CTACCTGCCGAGCTTCTGCA	TGG	0.9019	54	37	-	8	2	100635576
+TSPAN6	CRISPRko	ACTTTGTCTGCATCTCTCTG	TGG	0.5095	50	38	+	14	5	100633432
+TSPAN6	CRISPRko	CCATTAGGGTTGTTTTATAA	AGG	-1.757	11	39	-	57	6	100632559
+TSPAN6	CRISPRko	GGCGTTATCCTTCTTGCAGT	TGG	0.1046	47	40	-	22	2	100635724
+TSPAN6	CRISPRko	CTCATACTGGTTATTTGTTA	TGG	-1.414	21	41	+	49	7	100630824
+TSPAN6	CRISPRko	ACTAATTATTACTCAGAAAA	AGG	-1.762	12	42	-	58	5	100633481
+TSPAN6	CRISPRko	GAACTGGTCGCTGCCATCGT	AGG	0.4235	53	43	-	17	3	100635203
+TSPAN6	CRISPRko	TGCAGTTGGCATTTGGGGCA	AGG	-0.6226	38	44	-	36	2	100635710
+TSPAN6	CRISPRko	AGGATTTGTTTTCAGACATG	AGG	-0.6513	39	45	-	37	3	100635183
+TSPAN6	CRISPRko	CTTCTTGCAGTTGGCATTTG	GGG	-0.9061	33	46	-	43	2	100635715
+TSPAN6	CRISPRko	ATTATTCTTTTGGGCACCTT	TGG	-1.326	29	47	-	48	2	100635607
+TSPAN6	CRISPRko	AAAGAGAAAAGTAATTCTCC	AGG	0.2222	59	48	+	20	2	100635694
+TSPAN6	CRISPRko	GACCATTATAGAGTCAGAAA	TGG	-0.3101	56	49	-	30	6	100632532
+TSPAN6	CRISPRko	GTTTCTGACTCTCGTTTTTT	TGG	-1.928	27	50	-	60	3	100635228
+TSPAN6	CRISPRko	TCCTTCTTGCAGTTGGCATT	TGG	-0.9616	46	51	-	44	2	100635717
+TSPAN6	CRISPRko	CATTTGGGGCAAGGTGAGCC	TGG	-0.7729	55	52	-	39	2	100635701
+TSPAN6	CRISPRko	GTCGTTGCAGGAATTTCCTT	TGG	-0.7847	58	53	-	40	6	100632507
+TSPAN6	CRISPRko	AATCTACACTTTTATTTTCT	GGG	-1.728	43	54	-	56	1	100636613
+TSPAN6	CRISPRko	CTATTTTATTTCTAGCTGAT	TGG	-1.77	42	55	-	59	7	100630865
+TSPAN6	CRISPRko	TCAAGTTTACAGCAACTCTT	AGG	-0.8362	60	56	+	41	5	100633465
+TSPAN6	CRISPRko	GTTTTTCAGTTGCATTGTTG	TGG	-1.647	48	57	-	53	5	100633532
+TSPAN6	CRISPRko	TAATCTACACTTTTATTTTC	TGG	-1.711	51	58	-	55	1	100636614
+TSPAN6	CRISPRko	CTTTAAGAATAATTATGAGA	AGG	-1.083	61	59	-	46	4	100634002
+TSPAN6	CRISPRko	GGTACCGTCATTATTCTTTT	GGG	-1.565	57	60	-	51	2	100635616
+TSPAN6	CRISPRko	CTTTTCTCTTTTAAATGAGA	AGG	-1.957	52	61	-	61	2	100635671
+TNMD	CRISPRko	CTAGTAAAGGTTCACAGACG	CGG	1.02	5	1	-	5	7	100599600
+TNMD	CRISPRko	AGTGGGATTGATCCAATACA	TGG	0.8554	4	2	-	8	5	100597631
+TNMD	CRISPRko	TCACGCCAGACAAGCAAGTG	AGG	0.5136	2	3	+	19	6	100599154
+TNMD	CRISPRko	TATGGGTAGTAGCCTAGTAA	AGG	0.4736	6	4	-	22	7	100599613
+TNMD	CRISPRko	AAATGGCACTGATGAAACAT	TGG	0.6736	21	5	+	13	3	100594010
+TNMD	CRISPRko	GTCATACCTGATATTAGAGT	GGG	0.2888	7	6	-	28	5	100597648
+TNMD	CRISPRko	CACAGACGCGGCGGCAATAG	CGG	0.3928	16	7	-	24	7	100599588
+TNMD	CRISPRko	CCATATCCATACTGCTACCA	AGG	0.3583	18	8	+	26	7	100599642
+TNMD	CRISPRko	AAACAGGACAATTAGAGTTA	GGG	0.186	13	9	-	33	2	100585307
+TNMD	CRISPRko	CTTGGTAGCAGTATGGATAT	GGG	-0.04682	10	10	-	41	7	100599630
+TNMD	CRISPRko	AAAACAGGACAATTAGAGTT	AGG	-0.01437	11	11	-	40	2	100585308
+TNMD	CRISPRko	GGACAATTAGAGTTAGGGCC	AGG	0.04971	12	12	-	39	2	100585302
+TNMD	CRISPRko	GGCAATAGCGGTTGCCTCGA	CGG	0.1662	22	13	-	35	7	100599576
+TNMD	CRISPRko	TACACTGGCATCTACTTCGT	GGG	0.4776	38	14	+	21	4	100594281
+TNMD	CRISPRko	CCTTGGTAGCAGTATGGATA	TGG	-0.2211	8	15	-	53	7	100599631
+TNMD	CRISPRko	TCATCATGCCTTGTAACTGG	TGG	0.709	51	16	+	11	7	100599685
+TNMD	CRISPRko	GTGATAACGTGACCATGTAT	TGG	-0.1261	17	17	+	45	5	100597630
+TNMD	CRISPRko	ATCAATCCCACTCTAATATC	AGG	-0.4598	1	18	+	63	5	100597653
+TNMD	CRISPRko	TGGTGGGTGGCCCGCATGCT	GGG	-0.4237	3	19	+	62	7	100599702
+TNMD	CRISPRko	TGAGTTACAAGACTTTGAGG	AGG	0.9635	59	20	+	7	6	100599037
+TNMD	CRISPRko	AATAGAATTTGATCCCATGC	TGG	-0.1038	24	21	+	43	7	100599536
+TNMD	CRISPRko	TAACCTCTCTCATCCAGCAT	GGG	0.1678	36	22	-	34	7	100599538
+TNMD	CRISPRko	GATCCCATGCTGGATGAGAG	AGG	0.6974	60	23	+	12	7	100599546
+TNMD	CRISPRko	TGTCATACCTGATATTAGAG	TGG	0.1986	40	24	-	32	5	100597649
+TNMD	CRISPRko	ATTAGACCCTCCCCAGCATG	CGG	0.3615	53	25	-	25	7	100599702
+TNMD	CRISPRko	TGTTGTATTTACTGCCGTCG	AGG	-0.1093	34	26	+	44	7	100599573
+TNMD	CRISPRko	AGTGCTTGCTCCCCCAAAAC	AGG	-0.3355	20	27	-	58	2	100585323
+TNMD	CRISPRko	GAGCAAGCACTTCTGGCCGG	AGG	1.106	77	28	+	3	2	100585346
+TNMD	CRISPRko	AGAGAAGAAGAAGATTTACA	TGG	0.6484	64	29	+	17	3	100593947
+TNMD	CRISPRko	CATGCTGGGGAGGGTCTAAT	AGG	-1.075	9	30	+	73	7	100599716
+TNMD	CRISPRko	CGCGTCTGTGAACCTTTACT	AGG	-0.3171	25	31	+	57	7	100599612
+TNMD	CRISPRko	AAGATTTGTGGACTGGTGTT	TGG	-0.2152	31	32	+	51	2	100585287
+TNMD	CRISPRko	GAAAGTGTGCTCCATGTCAT	AGG	-0.3693	23	33	-	60	3	100593902
+TNMD	CRISPRko	CATCATGCCTTGTAACTGGT	GGG	0.2903	58	34	+	27	7	100599686
+TNMD	CRISPRko	GACAGATGACTCGTCCTCCT	TGG	-0.04979	43	35	-	42	7	100599648
+TNMD	CRISPRko	TGTAAATCACTTAAGATTTG	TGG	-0.6524	15	36	+	70	2	100585275
+TNMD	CRISPRko	ATAACCTCTCTCATCCAGCA	TGG	0.1013	49	37	-	37	7	100599539
+TNMD	CRISPRko	TGACTGTTCAAAGAAAGTTG	TGG	0.7401	76	38	-	10	5	100597523
+TNMD	CRISPRko	CATGCCTTGTAACTGGTGGG	TGG	0.6654	72	39	+	15	7	100599689
+TNMD	CRISPRko	GGTGGGTGGCCCGCATGCTG	GGG	0.98	83	40	+	6	7	100599703
+TNMD	CRISPRko	TTAGACCCTCCCCAGCATGC	GGG	0.654	73	41	-	16	7	100599701
+TNMD	CRISPRko	ATACACTGGCATCTACTTCG	TGG	0.6142	71	42	+	18	4	100594280
+TNMD	CRISPRko	TAACTCACATAGTCATTTAT	TGG	-1.199	14	43	-	76	6	100599174
+TNMD	CRISPRko	GTTCTCCCAGGCCTATGACA	TGG	1.105	86	44	+	4	3	100593902
+TNMD	CRISPRko	GTAAAGGTTCACAGACGCGG	CGG	1.151	89	45	-	2	7	100599597
+TNMD	CRISPRko	CACTTGCTTGTCTGGCGTGA	CGG	-1.076	19	46	-	74	6	100599140
+TNMD	CRISPRko	ATCACTTAAGATTTGTGGAC	TGG	-0.5166	30	47	+	64	2	100585280
+TNMD	CRISPRko	GAGCACACTTTCTACAGCAA	TGG	0.5026	75	48	+	20	3	100593924
+TNMD	CRISPRko	TCTGTTTTATAGGGATACAC	TGG	0.272	68	49	+	29	4	100594266
+TNMD	CRISPRko	TATCCATACTGCTACCAAGG	AGG	1.372	97	50	+	1	7	100599645
+TNMD	CRISPRko	CGGGCCACCCACCAGTTACA	AGG	0.263	69	51	-	30	7	100599682
+TNMD	CRISPRko	GCTTCTGAATATTTCAGTTC	TGG	-1.055	27	52	-	72	3	100593974
+TNMD	CRISPRko	TGGACTGGTGTTTGGTATCC	TGG	-0.5394	35	53	+	67	2	100585295
+TNMD	CRISPRko	GTGTCATCATGCCTTGTAAC	TGG	-0.3072	47	54	+	56	7	100599682
+TNMD	CRISPRko	TCTTTGAACAGTCAGTGATT	TGG	-1.189	28	55	+	75	5	100597543
+TNMD	CRISPRko	TGACAGTCTTCACAATTCTC	TGG	-0.7203	37	56	-	71	1	100585040
+TNMD	CRISPRko	ACCAGAAGAGGAAATAGATG	AGG	0.8425	102	57	+	9	4	100594358
+TNMD	CRISPRko	CTCTAATTGTCCTGTTTTGG	GGG	-1.245	32	58	+	79	2	100585324
+TNMD	CRISPRko	GGATTGAACAAAATGAACAG	TGG	0.6689	98	59	+	14	6	100599099
+TNMD	CRISPRko	TGTGCTCCATGTCATAGGCC	TGG	-0.5286	46	60	-	66	3	100593897
+TNMD	CRISPRko	ACTTGCTTGTCTGGCGTGAC	GGG	-0.6074	45	61	-	68	6	100599139
+TNMD	CRISPRko	GATTGAACAAAATGAACAGT	GGG	-0.1949	63	62	+	50	6	100599100
+TNMD	CRISPRko	GGGGAGCAAGCACTTCTGGC	CGG	0.1577	78	63	+	36	2	100585343
+TNMD	CRISPRko	ACTCTAATTGTCCTGTTTTG	GGG	-1.401	33	64	+	84	2	100585323
+TNMD	CRISPRko	CAGTCTTCACAATTCTCTGG	AGG	0.074	79	65	-	38	1	100585037
+TNMD	CRISPRko	GGTGGCCCGCATGCTGGGGA	GGG	0.2523	88	66	+	31	7	100599707
+TNMD	CRISPRko	TTTCAATAGGCTTTTCTGCT	GGG	-1.688	29	67	-	92	5	100597557
+TNMD	CRISPRko	TCTTCTGGTTCAGAAAATTC	AGG	-0.629	52	68	-	69	4	100594333
+TNMD	CRISPRko	AGAACTGAAATATTCAGAAG	CGG	0.4532	99	69	+	23	3	100593987
+TNMD	CRISPRko	CTTACTTTTTTGGGTACCTC	CGG	-1.253	48	70	-	80	2	100585351
+TNMD	CRISPRko	CGTCCTCCTTGGTAGCAGTA	TGG	-0.3677	70	71	-	59	7	100599637
+TNMD	CRISPRko	ATCCCTTTTTTTTCGTTGGC	AGG	-2.345	26	72	-	103	6	100599069
+TNMD	CRISPRko	GTCTTCTCTACTTTCACTTG	AGG	-1.234	54	73	-	77	6	100599117
+TNMD	CRISPRko	CTGGTGGGTGGCCCGCATGC	TGG	-0.5198	67	74	+	65	7	100599701
+TNMD	CRISPRko	TTTCCTGCCAACGAAAAAAA	AGG	-1.575	44	75	+	88	6	100599077
+TNMD	CRISPRko	TAACTCTAATTGTCCTGTTT	TGG	-1.62	42	76	+	90	2	100585321
+TNMD	CRISPRko	CTTTGAACAGTCAGTGATTT	GGG	-1.795	39	77	+	94	5	100597544
+TNMD	CRISPRko	TTTTCAATAGGCTTTTCTGC	TGG	-1.722	41	78	-	93	5	100597558
+TNMD	CRISPRko	TTCCTGCCAACGAAAAAAAA	GGG	-1.243	57	79	+	78	6	100599078
+TNMD	CRISPRko	CTCTGGAGGATTCTTTGCCA	TGG	-0.3836	74	80	-	61	1	100585023
+TNMD	CRISPRko	GTTACAAGACTTTGAGGAGG	AGG	-0.2155	84	81	+	52	6	100599040
+TNMD	CRISPRko	GTGCTCCATGTCATAGGCCT	GGG	-0.2753	82	82	-	54	3	100593896
+TNMD	CRISPRko	AATATTTCAGTTCTGGTCAC	AGG	-0.1885	90	83	-	49	3	100593967
+TNMD	CRISPRko	TTACAAGACTTTGAGGAGGA	GGG	-0.3019	85	84	+	55	6	100599041
+TNMD	CRISPRko	TGAACAAAATGAACAGTGGG	TGG	-0.1529	94	85	+	47	6	100599103
+TNMD	CRISPRko	ATCACAAATCTCCAGAATTT	TGG	-1.432	55	86	-	86	5	100597601
+TNMD	CRISPRko	GAAATATTCAGAAGCGGAAA	TGG	-0.1361	95	87	+	46	3	100593993
+TNMD	CRISPRko	AGAAAATCTCGGTTTTCAAT	AGG	-1.953	50	88	-	96	5	100597570
+TNMD	CRISPRko	TGAATTTTCTGAACCAGAAG	AGG	-1.428	62	89	+	85	4	100594346
+TNMD	CRISPRko	GGGTGGCCCGCATGCTGGGG	AGG	-0.1825	100	90	+	48	7	100599706
+TNMD	CRISPRko	TCTTCTCTACTTTCACTTGA	GGG	-1.56	61	91	-	87	6	100599116
+TNMD	CRISPRko	TCTTAAAAATTCCAAAATTC	TGG	-1.826	56	92	+	95	5	100597601
+TNMD	CRISPRko	TGGAATTTTTAAGAAAATCT	CGG	-1.644	66	93	-	91	5	100597581
+TNMD	CRISPRko	AAGTGATTTACATATTTTCT	TGG	-2.187	65	94	-	101	2	100585253
+TNMD	CRISPRko	TTCTTCCTCACTTGCTTGTC	TGG	-1.334	92	95	-	82	6	100599148
+TNMD	CRISPRko	ACCTCATCTATTTCCTCTTC	TGG	-1.581	87	96	-	89	4	100594348
+TNMD	CRISPRko	TTCTGAGTTACAAGACTTTG	AGG	-1.362	96	97	+	83	6	100599034
+TNMD	CRISPRko	TTCAATCCCTTTTTTTTCGT	TGG	-2.185	80	98	-	100	6	100599073
+TNMD	CRISPRko	TGTGTATTTACTTACTTTTT	TGG	-2.183	81	99	-	99	2	100585361
+TNMD	CRISPRko	TTTGGGGGAGCAAGCACTTC	TGG	-1.329	101	100	+	81	2	100585339
+TNMD	CRISPRko	TTCTTCTTTCAGACTGAAAA	TGG	-2.038	93	101	+	98	7	100599513
+TNMD	CRISPRko	AACTCTAATTGTCCTGTTTT	GGG	-2.231	91	102	+	102	2	100585322
+TNMD	CRISPRko	GTGTATTTACTTACTTTTTT	GGG	-2.032	103	103	-	97	2	100585360
+DPM1	CRISPRko	AGAACCTGCCGCTCATCGTG	TGG	1.083	5	1	-	2	1	50958395
+DPM1	CRISPRko	TTCTCGCGCTCGTTGTAGGT	AGG	0.6405	1	2	+	11	1	50958425
+DPM1	CRISPRko	TGGAAGCCCAGATGGAACAA	GGG	1.31	13	3	-	1	2	50955236
+DPM1	CRISPRko	ACCAGCAGCCACACGATGAG	CGG	0.8762	18	4	+	6	1	50958398
+DPM1	CRISPRko	GCAGCCACACGATGAGCGGC	AGG	0.6978	15	5	+	9	1	50958402
+DPM1	CRISPRko	ACTACGACTGACTTCCAAGG	AGG	0.6742	20	6	+	10	1	50958514
+DPM1	CRISPRko	CGAGAGAAAAAGTTGGGACT	AGG	0.2411	4	7	-	28	3	50948634
+DPM1	CRISPRko	AATAATGATGTAGTTTCCTG	TGG	0.9115	28	8	+	5	4	50945888
+DPM1	CRISPRko	AAGACCACGAGAGAAAAAGT	TGG	0.311	14	9	-	25	3	50948641
+DPM1	CRISPRko	CATGGAATGAAACATGCCAC	AGG	0.1326	11	10	-	29	4	50945893
+DPM1	CRISPRko	CAGGTTCTCGCGCTCGTTGT	AGG	0.2743	17	11	+	26	1	50958421
+DPM1	CRISPRko	AGACCACGAGAGAAAAAGTT	GGG	-0.1436	7	12	-	38	3	50948640
+DPM1	CRISPRko	AGACCTGGTGCGCTACCTCT	CGG	0.41	25	13	+	20	1	50958364
+DPM1	CRISPRko	GTTGAATTATACTATTGGCG	AGG	1.051	43	14	-	3	8	50936153
+DPM1	CRISPRko	TGGTGAAAAGCTTCTCCGAG	AGG	0.4907	31	15	-	16	1	50958368
+DPM1	CRISPRko	AAGGGATGTTGCTGAACAGT	TGG	0.3641	27	16	-	21	2	50955218
+DPM1	CRISPRko	TTAGGAACTGCATATATTCA	TGG	-0.3799	3	17	-	47	4	50945911
+DPM1	CRISPRko	ATGGAAGCCCAGATGGAACA	AGG	0.263	23	18	-	27	2	50955237
+DPM1	CRISPRko	ATAGATGATGGAAGCCCAGA	TGG	0.5295	35	19	-	15	2	50955244
+DPM1	CRISPRko	ACCGAATATTTGTTCTGTCG	TGG	-0.3496	6	20	+	45	1	50958455
+DPM1	CRISPRko	AGTACCTACCTAATAAATTC	AGG	-0.4467	2	21	+	51	5	50945745
+DPM1	CRISPRko	AGCTCCCGCCGAGACCTGCG	AGG	0.4195	34	22	+	19	1	50958491
+DPM1	CRISPRko	AAGTCAGTCGTAGTCCTCGC	AGG	-0.3068	10	23	-	43	1	50958494
+DPM1	CRISPRko	GAACAGTTGGAGAAGATCTA	TGG	-0.3336	12	24	-	44	2	50955205
+DPM1	CRISPRko	TCCACGACAGAACAAATATT	CGG	-0.4351	9	25	-	49	1	50958445
+DPM1	CRISPRko	AGGACTACGACTGACTTCCA	AGG	0.007545	26	26	+	35	1	50958511
+DPM1	CRISPRko	TATGGTGAATCCAAGTTGGG	AGG	0.5781	50	27	-	12	9	50935193
+DPM1	CRISPRko	AAATGGAGGTGTATATGGCT	GGG	0.5525	49	28	-	13	6	50942059
+DPM1	CRISPRko	GTCTCTGGAACTCGCTACAA	AGG	0.3306	39	29	-	24	6	50942082
+DPM1	CRISPRko	AACAGTTGGAGAAGATCTAT	GGG	-0.6119	8	30	-	55	2	50955204
+DPM1	CRISPRko	TAGTCCTCGCAGGTCTCGGC	GGG	0.09252	33	31	-	30	1	50958484
+DPM1	CRISPRko	CCTGTTAAATCAGATGCTCC	TGG	0.3418	42	32	+	23	7	50940891
+DPM1	CRISPRko	GAGTTAAAAAATTGGCCCCA	CGG	0.5505	51	33	+	14	7	50940928
+DPM1	CRISPRko	ACTCGCTACAAAGGAAATGG	AGG	0.9155	63	34	-	4	6	50942073
+DPM1	CRISPRko	TAAAGGCTACGTCTTCCAGA	TGG	0.7237	60	35	-	8	8	50936198
+DPM1	CRISPRko	GCCGCTCATCGTGTGGCTGC	TGG	-0.02621	32	36	-	36	1	50958388
+DPM1	CRISPRko	ATTCTACAGGAAGCAAAAGG	AGG	0.4793	52	37	-	17	6	50942119
+DPM1	CRISPRko	TATTCCTGAATTTATTAGGT	AGG	-0.4949	16	38	-	53	5	50945738
+DPM1	CRISPRko	GACTTCCAAGGAGGCCATGG	CGG	0.05382	37	39	+	32	1	50958523
+DPM1	CRISPRko	GTAGTCCTCGCAGGTCTCGG	CGG	0.01836	36	40	-	34	1	50958485
+DPM1	CRISPRko	GTTTATGGTGAATCCAAGTT	GGG	0.06266	41	41	-	31	9	50935196
+DPM1	CRISPRko	GAAAAGAAAAATAATCAGGT	AGG	0.7467	72	42	-	7	6	50942032
+DPM1	CRISPRko	GTCGTAGTCCTCGCAGGTCT	CGG	-0.5495	29	43	-	54	1	50958488
+DPM1	CRISPRko	CAACATCCCTTGTTCCATCT	GGG	-0.8164	21	44	+	64	2	50955241
+DPM1	CRISPRko	TCGCAGGTCTCGGCGGGAGC	TGG	-0.4416	38	45	-	50	1	50958478
+DPM1	CRISPRko	ACTCAGATCTTGCTGAGACC	AGG	0.3515	67	46	-	22	7	50940898
+DPM1	CRISPRko	TTCTACAGGAAGCAAAAGGA	GGG	0.4632	71	47	-	18	6	50942118
+DPM1	CRISPRko	AGGAAACTACATCATTATTA	TGG	-0.851	24	48	-	65	4	50945873
+DPM1	CRISPRko	CCCGAACAATCATCTCCATC	TGG	-0.04381	55	49	+	37	8	50936194
+DPM1	CRISPRko	GCAACATCCCTTGTTCCATC	TGG	-1.336	19	50	+	74	2	50955240
+DPM1	CRISPRko	CCAGATGGAGATGATTGTTC	GGG	0.05259	62	51	-	33	8	50936183
+DPM1	CRISPRko	AGTCCCAACTTTTTCTCTCG	TGG	-1.365	22	52	+	75	3	50948648
+DPM1	CRISPRko	GAAATTATAATCATAGATGA	TGG	-0.1934	59	53	-	39	2	50955256
+DPM1	CRISPRko	TGTTTATGGTGAATCCAAGT	TGG	-0.3948	54	54	-	48	9	50935197
+DPM1	CRISPRko	ACACGATCCACAAATGATAT	TGG	-0.8092	40	55	+	63	9	50935228
+DPM1	CRISPRko	AGACAGTTGAATTATACTAT	TGG	-0.6719	44	56	-	59	8	50936158
+DPM1	CRISPRko	CAAGATCTGAGTTAAAAAAT	TGG	-0.6584	47	57	+	58	7	50940920
+DPM1	CRISPRko	AAAGGAAATGGAGGTGTATA	TGG	-0.7071	45	58	-	60	6	50942064
+DPM1	CRISPRko	CTAGGTTCCAATATCATTTG	TGG	-0.357	61	59	-	46	9	50935224
+DPM1	CRISPRko	AATTTATTCCTGAATTTATT	AGG	-1.524	30	60	-	77	5	50945742
+DPM1	CRISPRko	TCCAGATGGAGATGATTGTT	CGG	-0.2852	68	61	-	42	8	50936184
+DPM1	CRISPRko	CCAGGAGCATCTGATTTAAC	AGG	-0.4594	58	62	-	52	7	50940880
+DPM1	CRISPRko	GGAACTCGCTACAAAGGAAA	TGG	-0.2829	70	63	-	41	6	50942076
+DPM1	CRISPRko	TGTTTTATTTGGCAGCCGTG	GGG	-0.6437	56	64	-	57	7	50940932
+DPM1	CRISPRko	ATTGTTTTATTTGGCAGCCG	TGG	-1.042	48	65	-	69	7	50940934
+DPM1	CRISPRko	ACTGACTTCCAAGGAGGCCA	TGG	-0.2269	77	66	+	40	1	50958520
+DPM1	CRISPRko	ATTTGAAAAGAAAAATAATC	AGG	-1.017	53	67	-	66	6	50942036
+DPM1	CRISPRko	TCATTTGTGGATCGTGTTTA	TGG	-1.577	46	68	-	78	9	50935211
+DPM1	CRISPRko	TTGTTTTATTTGGCAGCCGT	GGG	-1.029	57	69	-	67	7	50940933
+DPM1	CRISPRko	CTGATTTAACAGGAAGTTTC	AGG	-0.8014	69	70	-	62	7	50940870
+DPM1	CRISPRko	TTTATTCTACAGGAAGCAAA	AGG	-0.6351	78	71	-	56	6	50942122
+DPM1	CRISPRko	GAAATGGAGGTGTATATGGC	TGG	-0.7617	75	72	-	61	6	50942060
+DPM1	CRISPRko	ATTTCTCTAGAACTTCTTTT	CGG	-1.323	66	73	+	73	8	50936251
+DPM1	CRISPRko	GGTAATTTTGATATTGTCTC	TGG	-1.457	64	74	-	76	6	50942097
+DPM1	CRISPRko	ATAGAAAAATGTGTTTCTAA	AGG	-1.086	73	75	-	70	8	50936215
+DPM1	CRISPRko	GAAATAGTATCTTTCTTGAA	AGG	-1.709	65	76	-	79	9	50935166
+DPM1	CRISPRko	TATTTCATTTCCTCCCAACT	TGG	-1.038	76	77	+	68	9	50935194
+DPM1	CRISPRko	CAGTTCCGCCATGGCCTCCT	TGG	-1.312	74	78	-	72	1	50958517
+DPM1	CRISPRko	ATAAATTCAGGAATAAATTT	TGG	-1.291	79	79	+	71	5	50945757
+SCYL3	CRISPRko	TGGAATCGCTAGTATCACGC	AGG	1.119	1	1	+	10	11	169859196
+SCYL3	CRISPRko	TGATACTAGCGATTCCATTG	TGG	0.8602	4	2	-	22	11	169859179
+SCYL3	CRISPRko	GATCACCCGTGACTGGAACA	GGG	1.308	27	3	+	3	10	169862753
+SCYL3	CRISPRko	TTAGATACTAAAGTAAACCC	AGG	1.117	29	4	-	11	12	169854691
+SCYL3	CRISPRko	GAAGAAAGTCATCTTGCCAC	AGG	0.6759	7	5	-	35	10	169862618
+SCYL3	CRISPRko	GCTGTCCCCTGGCTTACCTC	TGG	0.8899	23	6	+	20	4	169875978
+SCYL3	CRISPRko	AGAGTGTCATGGACATGCCC	GGG	0.9437	31	7	-	18	6	169870318
+SCYL3	CRISPRko	CAAGGTTAATAAAGCTGCCA	AGG	0.5819	9	8	-	43	2	169888681
+SCYL3	CRISPRko	ATCTTACCATCTCTTCAGGA	GGG	0.6582	17	9	+	36	5	169873706
+SCYL3	CRISPRko	TATCCCGCTGTACTGCAAGA	TGG	0.5567	25	10	-	45	2	169888746
+SCYL3	CRISPRko	CAGAGTCAGCTGCTTGTCAG	AGG	0.5505	36	11	-	46	9	169864480
+SCYL3	CRISPRko	AGATTAGCCTTGTACAAAGG	GGG	0.2688	11	12	-	74	12	169854558
+SCYL3	CRISPRko	CCCCTGGCTTACCTCTGGTG	TGG	0.9479	70	13	+	16	4	169875983
+SCYL3	CRISPRko	GATGGACACTGGAAGCTAGG	AGG	1.221	79	14	-	7	4	169876024
+SCYL3	CRISPRko	TTGTGAGTGAAGATGGACAC	TGG	1.124	80	15	-	8	4	169876035
+SCYL3	CRISPRko	ATAAAAATCTTAGCAAGCAA	GGG	0.4822	48	16	+	50	3	169878794
+SCYL3	CRISPRko	GATACTAAAGTAAACCCAGG	AGG	0.4345	46	17	-	57	12	169854688
+SCYL3	CRISPRko	GGATAAACAGCAAGTCCAGA	GGG	0.7385	77	18	+	29	2	169888775
+SCYL3	CRISPRko	TGGGACTTGACATCATCACA	AGG	0.4691	57	19	+	51	12	169854780
+SCYL3	CRISPRko	AGATGTAAAAAATACTTCGG	AGG	0.2871	40	20	-	71	12	169854902
+SCYL3	CRISPRko	GATTAGCCTTGTACAAAGGG	GGG	0.2316	33	21	-	79	12	169854557
+SCYL3	CRISPRko	CAAACTTGGCCTCTGACCTG	TGG	1.038	99	22	+	13	10	169862613
+SCYL3	CRISPRko	GGGATAAACAGCAAGTCCAG	AGG	0.9441	102	23	+	17	2	169888774
+SCYL3	CRISPRko	CTGAGTGAAGTGCTCCACGT	AGG	1.014	108	24	+	14	10	169862660
+SCYL3	CRISPRko	TTTATCTTGTACTGTGGAAG	CGG	0.1695	42	25	-	81	3	169878765
+SCYL3	CRISPRko	GTCTCACATCGAGGCCTACG	TGG	0.91	107	26	-	19	10	169862663
+SCYL3	CRISPRko	CTCTGTCCAGCAGAAATCTG	AGG	0.4515	72	27	+	55	9	169864509
+SCYL3	CRISPRko	GAAGAAGTCATGAGATAGTA	AGG	0.1127	37	28	+	91	7	169868947
+SCYL3	CRISPRko	GGACCAGAGGTGGTTGTGGG	AGG	0.7149	98	29	-	31	11	169859115
+SCYL3	CRISPRko	TCATGAAGAGCATGTGCGGA	TGG	0.3551	65	30	-	64	10	169862693
+SCYL3	CRISPRko	TTTTCGGGTAGATCATGCGC	AGG	-0.09705	22	31	-	108	10	169862792
+SCYL3	CRISPRko	ATCAGAACTTGGTTTAGGAG	AGG	0.1379	47	32	-	87	12	169854473
+SCYL3	CRISPRko	CCAGTGCACTACCTTGGATG	TGG	0.8168	110	33	-	24	12	169854749
+SCYL3	CRISPRko	TTTCGGGTAGATCATGCGCA	GGG	0.4687	82	34	-	52	10	169862791
+SCYL3	CRISPRko	TATTGTGTGTTAGGTGTCCC	TGG	0.3405	69	35	+	66	4	169876090
+SCYL3	CRISPRko	CTACGTGGAGCACTTCACTC	AGG	0.7465	109	36	-	28	10	169862648
+SCYL3	CRISPRko	TGAATCCCATTCCAAAATGT	CGG	0.09425	43	37	-	94	7	169868972
+SCYL3	CRISPRko	TCTGCATAGCCTAGCAGTGC	TGG	0.4572	84	38	-	54	11	169859149
+SCYL3	CRISPRko	TCACTGAAGAGTCTATGCCT	TGG	0.2786	67	39	-	73	12	169854600
+SCYL3	CRISPRko	GGCAGTGCGTTTGAAGATCT	TGG	-0.5046	8	40	+	135	11	169859095
+SCYL3	CRISPRko	GGGCATGTCCATGACACTCT	GGG	0.2272	63	41	+	80	6	169870332
+SCYL3	CRISPRko	CTCACAATCTTAAATGAACA	GGG	-0.3923	19	42	-	125	6	169870260
+SCYL3	CRISPRko	GGTGCTGCTGTCTCACATCG	AGG	1.317	145	43	-	1	10	169862672
+SCYL3	CRISPRko	CACTGAGCGAGTACAGCCCC	TGG	0.7073	115	44	-	32	3	169878726
+SCYL3	CRISPRko	CAAAAGTGTCATCACAAGAA	AGG	-0.5167	12	45	-	136	12	169854510
+SCYL3	CRISPRko	TAAAAGATCCTGAGATGGAT	TGG	-0.4721	20	46	-	131	12	169854420
+SCYL3	CRISPRko	ACGCAGGCCCAGCAAAACCT	AGG	0.6174	114	47	+	40	11	169859212
+SCYL3	CRISPRko	AAGTTCATGAAGAGCATGTG	CGG	1.247	149	48	-	6	10	169862697
+SCYL3	CRISPRko	CAAACAACTGGAGAAGCACG	GGG	1.299	151	49	+	5	10	169862731
+SCYL3	CRISPRko	ACAACTCTCCCAGAGTGTCA	TGG	0.6957	124	50	-	33	6	169870329
+SCYL3	CRISPRko	AGTTCTGATGGAACCTTAAG	GGG	0.0593	60	51	+	98	12	169854498
+SCYL3	CRISPRko	CACATCCAAGGTAGTGCACT	GGG	-0.7212	6	52	+	153	12	169854761
+SCYL3	CRISPRko	TGGGATCTATGACATATTGC	TGG	-0.02279	58	53	-	102	3	169878663
+SCYL3	CRISPRko	TTTGCCATCTTGCAGTACAG	CGG	0.1277	71	54	+	89	2	169888753
+SCYL3	CRISPRko	GGTTACTTACTCTGTCATGA	AGG	0.5663	117	55	+	44	3	169878641
+SCYL3	CRISPRko	TGCTGCAGCAGAAATTACTG	AGG	-0.2797	45	56	-	117	12	169854275
+SCYL3	CRISPRko	GGATGTGGAAGAGTCATCTT	GGG	-0.4624	34	57	-	128	12	169854734
+SCYL3	CRISPRko	GCTACAAAACCTGTTACCTC	AGG	-0.5911	24	58	-	141	12	169854655
+SCYL3	CRISPRko	CAAGTCCCAGTGCACTACCT	TGG	-0.178	53	59	-	112	12	169854755
+SCYL3	CRISPRko	ACCCGTGACTGGAACAGGGC	TGG	0.2807	94	60	+	72	10	169862757
+SCYL3	CRISPRko	AACAGACATTATTGTGTGTT	AGG	-0.6546	18	61	+	149	4	169876081
+SCYL3	CRISPRko	TCTCCTCCCACAACCACCTC	TGG	0.4481	112	62	+	56	11	169859123
+SCYL3	CRISPRko	GCACGGGGATCACCCGTGAC	TGG	-0.6365	21	63	+	147	10	169862746
+SCYL3	CRISPRko	GATAAAAATCTTAGCAAGCA	AGG	-0.3532	49	64	+	121	3	169878793
+SCYL3	CRISPRko	TCAGCAAACCAATCCATCTC	AGG	-0.318	52	65	+	119	12	169854423
+SCYL3	CRISPRko	ACCAGCCCTGTTCCAGTCAC	GGG	0.2438	96	66	-	76	10	169862747
+SCYL3	CRISPRko	AGAAAACTCTTAACAGCCAC	TGG	0.1479	87	67	+	85	9	169864412
+SCYL3	CRISPRko	AAAAGATTAGCCTTGTACAA	AGG	-0.6342	30	68	-	146	12	169854561
+SCYL3	CRISPRko	AAACTGTCAACATACAGATT	TGG	-0.845	15	69	-	161	12	169854801
+SCYL3	CRISPRko	GATAGTAAGGTGCAGAGCGC	TGG	0.7191	146	70	+	30	7	169868960
+SCYL3	CRISPRko	CCACATCCAAGGTAGTGCAC	TGG	-0.3652	54	71	+	123	12	169854760
+SCYL3	CRISPRko	CAGAGCGCTGGCCGACATTT	TGG	-1.038	3	72	+	174	7	169868972
+SCYL3	CRISPRko	TCCTCAGGTTCACTCCAGTC	AGG	0.09718	85	73	+	93	12	169854846
+SCYL3	CRISPRko	TAAGAGAGAAAATGAAGACA	AGG	1.307	174	74	-	4	2	169888699
+SCYL3	CRISPRko	CAGAGGAATTGATAGCTTCA	AGG	-0.4705	50	75	-	130	9	169864463
+SCYL3	CRISPRko	GAAGATGGACACTGGAAGCT	AGG	0.5914	140	76	-	41	4	169876027
+SCYL3	CRISPRko	TTCTTATATTACCTGAACTG	AGG	0.2981	113	77	-	69	12	169854357
+SCYL3	CRISPRko	TCAAACAACTGGAGAAGCAC	GGG	0.5836	141	78	+	42	10	169862730
+SCYL3	CRISPRko	CGGGCATGTCCATGACACTC	TGG	0.2394	111	79	+	77	6	169870331
+SCYL3	CRISPRko	GTTTCCAAAGCCACTTCCAG	GGG	0.2927	119	80	+	70	3	169878721
+SCYL3	CRISPRko	AAAGATTAGCCTTGTACAAA	GGG	-1.305	5	81	-	185	12	169854560
+SCYL3	CRISPRko	AGGGCTGGTGAGAGCAAGCA	AGG	0.6531	153	82	+	37	10	169862772
+SCYL3	CRISPRko	ACTAAAGTAAACCCAGGAGG	TGG	0.5162	144	83	-	47	12	169854685
+SCYL3	CRISPRko	TTTAACATTGAAGAGTGAAG	AGG	0.3565	128	84	-	63	8	169866921
+SCYL3	CRISPRko	TCAGTGAGTGAAAGCAAAGC	AGG	0.1026	100	85	+	92	12	169854627
+SCYL3	CRISPRko	GGTCTCTCTGCTTGGACCAG	AGG	0.8226	170	86	-	23	11	169859128
+SCYL3	CRISPRko	TATCTGCTTGGCCCCAAAAA	AGG	-0.7584	38	87	-	156	9	169864374
+SCYL3	CRISPRko	CCAGAGGGTAAGGTAAATGG	TGG	1.315	192	88	+	2	2	169888790
+SCYL3	CRISPRko	GCCTGACTGGAGTGAACCTG	AGG	0.7881	169	89	-	26	12	169854836
+SCYL3	CRISPRko	GCCTCTTCTGCTTAATCAGT	TGG	-0.337	75	90	-	120	9	169864435
+SCYL3	CRISPRko	CAAGTTCTGATGGAACCTTA	AGG	-1.026	26	91	+	172	12	169854496
+SCYL3	CRISPRko	ATCTATCCCTCCTGAAGAGA	TGG	0.3728	137	92	-	61	5	169873701
+SCYL3	CRISPRko	AGTTTCTCAGGCCACACCAG	AGG	1.119	189	93	-	9	4	169875983
+SCYL3	CRISPRko	ATTTGCTCCTAGGTTTTGCT	GGG	-0.7826	41	94	-	158	11	169859208
+SCYL3	CRISPRko	GCAGCAGAAGGCTTAATTTC	TGG	-1.331	13	95	+	187	12	169854393
+SCYL3	CRISPRko	TCTTGTACTGTGGAAGCGGA	TGG	0.7493	175	96	-	27	3	169878761
+SCYL3	CRISPRko	AATAGGCTTCTGCTCCCCTG	AGG	0.8858	184	97	+	21	12	169854650
+SCYL3	CRISPRko	ACACTTTTGGCGGCTCGATT	TGG	-1.625	2	98	+	204	12	169854535
+SCYL3	CRISPRko	GCAGAGAGACCAGCACTGCT	AGG	1.109	194	99	+	12	11	169859151
+SCYL3	CRISPRko	TACAAAACCTGTTACCTCAG	GGG	0.1463	122	100	-	86	12	169854653
+SCYL3	CRISPRko	CTACAAAACCTGTTACCTCA	GGG	-0.9678	39	101	-	169	12	169854654
+SCYL3	CRISPRko	TCTCATGACTTCTTCAGGTG	AGG	0.4856	159	102	-	49	7	169868928
+SCYL3	CRISPRko	GCGAGTACAGCCCCTGGAAG	TGG	0.7997	183	103	-	25	3	169878720
+SCYL3	CRISPRko	TACTTTAGTATCTAAGCTGC	TGG	-1.444	16	104	+	193	12	169854710
+SCYL3	CRISPRko	AGATGACTCTTCCACATCCA	AGG	0.4885	161	105	+	48	12	169854749
+SCYL3	CRISPRko	CTTTCTTGTGATGACACTTT	TGG	-1.534	10	106	+	201	12	169854522
+SCYL3	CRISPRko	ATGCAGAGTAATTGCCACAA	TGG	0.9542	197	107	+	15	11	169859176
+SCYL3	CRISPRko	GCCAGTAAAAGATCCTGAGA	TGG	-0.1197	104	108	-	109	12	169854425
+SCYL3	CRISPRko	CTCTCTGCTTGGACCAGAGG	TGG	0.3582	152	109	-	62	11	169859125
+SCYL3	CRISPRko	ACCAACTGATTAAGCAGAAG	AGG	0.6897	180	110	+	34	9	169864445
+SCYL3	CRISPRko	GTTCCATCAGAACTTGGTTT	AGG	-0.7469	62	111	-	154	12	169854478
+SCYL3	CRISPRko	ACACTGGAAGCTAGGAGGAA	TGG	0.3885	156	112	-	60	4	169876019
+SCYL3	CRISPRko	CGTCATCCCCCCTTTGTACA	AGG	-0.8675	55	113	+	163	12	169854562
+SCYL3	CRISPRko	GTTCTAAAAAGTCTGAGGAG	TGG	0.09383	123	114	-	95	12	169854858
+SCYL3	CRISPRko	TCTGGGAGAGTTGTGAATTC	TGG	-0.4656	89	115	+	129	6	169870349
+SCYL3	CRISPRko	TGGATGTGGAAGAGTCATCT	TGG	-0.6174	74	116	-	145	12	169854735
+SCYL3	CRISPRko	TGAGGAAAACTGCATCACTG	GGG	0.4608	168	117	+	53	12	169854314
+SCYL3	CRISPRko	CTTCATGAACTTCAAACAAC	TGG	-0.8044	64	118	+	159	10	169862719
+SCYL3	CRISPRko	ATCACAAGAAAGGCCCCTTA	AGG	-1.365	35	119	-	189	12	169854500
+SCYL3	CRISPRko	TTCAAACAACTGGAGAAGCA	CGG	0.1505	143	120	+	84	10	169862729
+SCYL3	CRISPRko	GGAATTGATAGCTTCAAGGT	TGG	0.6458	190	121	-	38	9	169864459
+SCYL3	CRISPRko	CTTGGACCAGAGGTGGTTGT	GGG	0.07809	132	122	-	97	11	169859118
+SCYL3	CRISPRko	AATATAAGAAAAGCAGCAGA	AGG	0.3481	166	123	+	65	12	169854381
+SCYL3	CRISPRko	TTGTACAAGGCTAATCTTTT	GGG	-2.388	14	124	+	217	12	169854575
+SCYL3	CRISPRko	CTTCAGGAGGGATAGATGCT	GGG	0.1667	150	125	+	82	5	169873718
+SCYL3	CRISPRko	TTCCAAATGAAAAGGCATCC	CGG	-0.561	97	126	+	138	6	169870311
+SCYL3	CRISPRko	TTCTGCTCCCCTGAGGTAAC	AGG	-1.049	61	127	+	175	12	169854657
+SCYL3	CRISPRko	AATACTGACCTTTCTCTAGA	AGG	-0.23	121	128	-	115	11	169859046
+SCYL3	CRISPRko	AACAGCAAGTCCAGAGGGTA	AGG	-0.2983	118	129	+	118	2	169888780
+SCYL3	CRISPRko	TTTGTACAAGGCTAATCTTT	TGG	-1.806	28	130	+	209	12	169854574
+SCYL3	CRISPRko	GGCATTTAGAAATGATTTTC	TGG	-1.325	51	131	-	186	8	169866966
+SCYL3	CRISPRko	AGTCCAGAGGGTAAGGTAAA	TGG	-0.4738	105	132	+	132	2	169888787
+SCYL3	CRISPRko	GCCCGGGATGCCTTTTCATT	TGG	-1.714	32	133	-	207	6	169870302
+SCYL3	CRISPRko	CACCAGCCCTGTTCCAGTCA	CGG	-0.0662	135	134	-	106	10	169862748
+SCYL3	CRISPRko	TGTACAAGGCTAATCTTTTG	GGG	-1.243	59	135	+	183	12	169854576
+SCYL3	CRISPRko	CTTAAGGTTCCATCAGAACT	TGG	-0.8567	86	136	-	162	12	169854484
+SCYL3	CRISPRko	GTTGGTGTTTGCAGAGCCAG	TGG	0.6215	210	137	-	39	9	169864417
+SCYL3	CRISPRko	TTTGAGGAAAACTGCATCAC	TGG	-1.218	68	138	+	182	12	169854312
+SCYL3	CRISPRko	GCTCACAATCTTAAATGAAC	AGG	-0.7481	95	139	-	155	6	169870261
+SCYL3	CRISPRko	CCTATTAAATTTCCCATAAA	TGG	-1.791	44	140	-	208	12	169854931
+SCYL3	CRISPRko	GACAGAAATGGTCCCAAAAA	AGG	-1.152	73	141	-	180	12	169854335
+SCYL3	CRISPRko	GCTTGGACCAGAGGTGGTTG	TGG	0.4258	195	142	-	58	11	169859119
+SCYL3	CRISPRko	TCCAAATGAAAAGGCATCCC	GGG	0.4166	196	143	+	59	6	169870312
+SCYL3	CRISPRko	TTGAGGAAAACTGCATCACT	GGG	-0.9495	90	144	+	167	12	169854313
+SCYL3	CRISPRko	CCACCATTTACCTTACCCTC	TGG	-0.4245	130	145	-	127	2	169888779
+SCYL3	CRISPRko	TCTTCTGCAGAGGTCTGTGC	TGG	0.1667	176	146	-	83	3	169878683
+SCYL3	CRISPRko	TCTCCTAAACCAAGTTCTGA	TGG	-0.4065	134	147	+	126	12	169854486
+SCYL3	CRISPRko	CGCTGGCCGACATTTTGGAA	TGG	-1.086	83	148	+	178	7	169868977
+SCYL3	CRISPRko	CTTCTGCAGAGGTCTGTGCT	GGG	-0.02175	163	149	-	101	3	169878682
+SCYL3	CRISPRko	TGTTTCCAAAGCCACTTCCA	GGG	0.1296	177	150	+	88	3	169878720
+SCYL3	CRISPRko	GTGCAAGGTCTGTTGAAAGC	TGG	0.2529	191	151	+	75	7	169869016
+SCYL3	CRISPRko	CAGCAGAAGGCTTAATTTCT	GGG	-1.424	76	152	+	191	12	169854394
+SCYL3	CRISPRko	TATCATCTTACCATCTCTTC	AGG	-1.069	91	153	+	176	5	169873702
+SCYL3	CRISPRko	TGGGGTAAGCTTGATTTCCA	AGG	-0.5265	133	154	+	137	12	169854594
+SCYL3	CRISPRko	TCCATCTCAGGATCTTTTAC	TGG	-1.663	66	155	+	205	12	169854435
+SCYL3	CRISPRko	AATTCTTACCTTCTAGAGAA	AGG	0.004629	171	156	+	100	11	169859049
+SCYL3	CRISPRko	CAGAGTGTCATGGACATGCC	CGG	-0.2025	158	157	-	113	6	169870319
+SCYL3	CRISPRko	GCTCTGGATTTCAGTTTCAG	CGG	0.0372	173	158	-	99	7	169869037
+SCYL3	CRISPRko	GCTGGCCGACATTTTGGAAT	GGG	-0.6172	129	159	+	144	7	169868978
+SCYL3	CRISPRko	ATTTCTCTGTCTAGTTTCTG	AGG	-0.03831	172	160	-	103	5	169873750
+SCYL3	CRISPRko	TAGCAGTGATTCCACCTCCT	GGG	-0.4876	142	161	+	134	12	169854685
+SCYL3	CRISPRko	GGGGAGACATCATCCTTTTT	TGG	-2.544	56	162	+	221	12	169854333
+SCYL3	CRISPRko	ACCTGAACTGAGGACAGAAA	TGG	-0.6678	127	163	-	150	12	169854347
+SCYL3	CRISPRko	CTCAGATGTAAAAAATACTT	CGG	-0.9933	106	164	-	171	12	169854905
+SCYL3	CRISPRko	CATCTTACCATCTCTTCAGG	AGG	0.08036	181	165	+	96	5	169873705
+SCYL3	CRISPRko	ATTCAGCAAAGTTGAGTGCA	AGG	0.3027	213	166	+	67	7	169869001
+SCYL3	CRISPRko	CCATTTATGGGAAATTTAAT	AGG	-1.529	81	167	+	200	12	169854942
+SCYL3	CRISPRko	CTTTTCATTTGGAACATTGG	TGG	-1.337	93	168	-	188	6	169870291
+SCYL3	CRISPRko	GGAAACATTGTCTTCTGCAG	AGG	0.2373	203	169	-	78	3	169878693
+SCYL3	CRISPRko	ACATCTGAGAGTCCATTTAT	GGG	-1.477	88	170	+	195	12	169854930
+SCYL3	CRISPRko	GAAGAGTGAAGAGGAGAAAA	CGG	0.3011	217	171	-	68	8	169866912
+SCYL3	CRISPRko	TACTATCTCATGACTTCTTC	AGG	-1.474	92	172	-	194	7	169868933
+SCYL3	CRISPRko	TCTTGTGATGACACTTTTGG	CGG	-1.304	103	173	+	184	12	169854525
+SCYL3	CRISPRko	ACATCATCACAAGGTTCTCT	AGG	-0.5844	147	174	+	140	12	169854789
+SCYL3	CRISPRko	CAGACTTTTTAGAACTTGAT	GGG	-1.971	78	175	+	212	12	169854877
+SCYL3	CRISPRko	GCAGTGCTGGTCTCTCTGCT	TGG	-0.211	178	176	-	114	11	169859136
+SCYL3	CRISPRko	ATGTTTCCAAAGCCACTTCC	AGG	-0.604	154	177	+	142	3	169878719
+SCYL3	CRISPRko	AAGTTCTGATGGAACCTTAA	GGG	-0.9381	131	178	+	166	12	169854497
+SCYL3	CRISPRko	AAGATTAGCCTTGTACAAAG	GGG	-0.1227	187	179	-	110	12	169854559
+SCYL3	CRISPRko	ACCATTTCTGTCCTCAGTTC	AGG	-0.8133	138	180	+	160	12	169854357
+SCYL3	CRISPRko	TCTTCAGGAGGGATAGATGC	TGG	0.1212	209	181	+	90	5	169873717
+SCYL3	CRISPRko	GGATCACCCGTGACTGGAAC	AGG	-0.4779	167	182	+	133	10	169862752
+SCYL3	CRISPRko	CATTTGCTCCTAGGTTTTGC	TGG	-1.373	116	183	-	190	11	169859209
+SCYL3	CRISPRko	AGTGAAAGCAAAGCAGGAAT	AGG	-0.04363	204	184	+	104	12	169854633
+SCYL3	CRISPRko	TTGCCATCTTGCAGTACAGC	GGG	-0.6817	157	185	+	151	2	169888754
+SCYL3	CRISPRko	GTAGCAGTGATTCCACCTCC	TGG	-0.2458	193	186	+	116	12	169854684
+SCYL3	CRISPRko	ACAGCCCCTGGAAGTGGCTT	TGG	-0.7196	160	187	-	152	3	169878714
+SCYL3	CRISPRko	TTGTTGCAGGGAGAGGCTGA	AGG	-0.09233	207	188	-	107	13	169853765
+SCYL3	CRISPRko	GGGAGACATCATCCTTTTTT	GGG	-2.383	101	189	+	216	12	169854334
+SCYL3	CRISPRko	TTTTGTCCTCAGATTTCTGC	TGG	-1.528	120	190	-	199	9	169864504
+SCYL3	CRISPRko	CACCAATGTTCCAAATGAAA	AGG	-0.883	155	191	+	164	6	169870303
+SCYL3	CRISPRko	AAAAGCATTCACCTTTTTTG	GGG	-1.489	125	192	+	196	9	169864374
+SCYL3	CRISPRko	ATCAAGTTCTAAAAAGTCTG	AGG	-0.3581	200	193	-	122	12	169854863
+SCYL3	CRISPRko	TCTGTGTTTGTGAGTGAAGA	TGG	-0.1681	211	194	-	111	4	169876042
+SCYL3	CRISPRko	TGAAGGCTGGGAAGAAGAAG	GGG	-0.06099	219	195	-	105	13	169853748
+SCYL3	CRISPRko	TGCCTTTTCATTTGGAACAT	TGG	-1.504	126	196	-	198	6	169870294
+SCYL3	CRISPRko	AGTCTGAGGAGTGGCCTGAC	TGG	-0.6475	185	197	-	148	12	169854849
+SCYL3	CRISPRko	GCAGGGAGAGGCTGAAGGCT	GGG	-0.3857	216	198	-	124	13	169853760
+SCYL3	CRISPRko	AACTGTTTGTAAAGTTTCTC	AGG	-1.122	162	199	-	179	4	169875995
+SCYL3	CRISPRko	AAGATTTTTATCTTGTACTG	TGG	-1.078	164	200	-	177	3	169878771
+SCYL3	CRISPRko	TTTTTGGGGCCAAGCAGATA	AGG	-0.6095	201	201	+	143	9	169864388
+SCYL3	CRISPRko	GTTTGATTTTCAGGCTCCTC	AGG	-1.535	148	202	+	202	12	169854831
+SCYL3	CRISPRko	ACTTTAGTATCTAAGCTGCT	GGG	-0.5823	214	203	+	139	12	169854711
+SCYL3	CRISPRko	TTCTGCTGCAGCAAATTTTG	AGG	-2.479	136	204	+	220	12	169854296
+SCYL3	CRISPRko	AGTATTTTTAGTAAAACTTG	GGG	-1.428	165	205	+	192	11	169859074
+SCYL3	CRISPRko	AAAAAGCATTCACCTTTTTT	GGG	-2.43	139	206	+	218	9	169864373
+SCYL3	CRISPRko	TGAACTGGGAAGATAATAAC	TGG	-1.032	198	207	-	173	13	169853722
+SCYL3	CRISPRko	AGAAGAAGGGGAGCTGAACT	GGG	-0.9575	208	208	-	168	13	169853736
+SCYL3	CRISPRko	AGTTTTCTTCCTTATCTGCT	TGG	-1.497	179	209	-	197	9	169864386
+SCYL3	CRISPRko	GCTGAAGGCTGGGAAGAAGA	AGG	-0.7808	220	210	-	157	13	169853750
+SCYL3	CRISPRko	TGCAGGGAGAGGCTGAAGGC	TGG	-0.9281	218	211	-	165	13	169853761
+SCYL3	CRISPRko	CTGAAGGCTGGGAAGAAGAA	GGG	-0.9709	215	212	-	170	13	169853749
+SCYL3	CRISPRko	TACATCTGAGAGTCCATTTA	TGG	-1.705	186	213	+	206	12	169854929
+SCYL3	CRISPRko	AATTTAATAGGCTGAGAAAA	TGG	-1.207	212	214	+	181	12	169854954
+SCYL3	CRISPRko	ATGTTGACAGTTTGATTTTC	AGG	-2.337	182	215	+	215	12	169854822
+SCYL3	CRISPRko	AAGAAGAAGGGGAGCTGAAC	TGG	-1.936	199	216	-	211	13	169853737
+SCYL3	CRISPRko	GAAAAAGCATTCACCTTTTT	TGG	-2.841	188	217	+	222	9	169864372
+SCYL3	CRISPRko	TCAGACTTTTTAGAACTTGA	TGG	-2.236	202	218	+	214	12	169854876
+SCYL3	CRISPRko	TGGCTTCTTTTTTACTTGAA	TGG	-2.024	206	219	+	213	12	169854455
+SCYL3	CRISPRko	CTTTATTTTGTTGCAGGGAG	AGG	-2.444	205	220	-	219	13	169853772
+SCYL3	CRISPRko	TCAGTATTTTTAGTAAAACT	TGG	-1.593	221	221	+	203	11	169859072
+SCYL3	CRISPRko	CAGTATTTTTAGTAAAACTT	GGG	-1.9	222	222	+	210	11	169859073
+FIRRM	CRISPRko	AGGTCTCAGAAACGACAACC	AGG	0.9158	19	1	+	12	13	169827836
+FIRRM	CRISPRko	GGCGTTTACTCAGTTCGCTG	AGG	0.6819	1	2	+	35	22	169849600
+FIRRM	CRISPRko	GATGAGCCACTATGGTGACA	TGG	0.9473	33	3	-	8	16	169830729
+FIRRM	CRISPRko	GCTAGAACAACATACCTTGG	AGG	1.002	35	4	+	7	22	169849579
+FIRRM	CRISPRko	GAACTGAGTAAACGCCTCCA	AGG	1.253	60	5	-	3	22	169849582
+FIRRM	CRISPRko	ACGCACCAGATGAGCCACTA	TGG	0.5942	21	6	-	45	16	169830737
+FIRRM	CRISPRko	TGCACACCATGTCACCATAG	TGG	0.3507	10	7	+	59	16	169830734
+FIRRM	CRISPRko	GCTGCTAGAACAACATACCT	TGG	0.924	59	8	+	11	22	169849576
+FIRRM	CRISPRko	AACTGTATCTTCAGATACAC	AGG	0.2866	13	9	+	64	11	169823468
+FIRRM	CRISPRko	CAAATATGTTGTACCGAGCA	AGG	0.1698	2	10	-	76	15	169830339
+FIRRM	CRISPRko	TAAGCTCAGGAGGTAACGCC	TGG	0.7431	55	11	-	25	18	169837001
+FIRRM	CRISPRko	GTGAGCAGCTGACTGATAAG	TGG	0.6237	37	12	-	43	12	169827142
+FIRRM	CRISPRko	AAGTATTGGCATGAAATGCA	TGG	1.478	79	13	-	1	8	169806056
+FIRRM	CRISPRko	TGCATCCATAGCTAACAAAG	AGG	0.6243	40	14	-	42	15	169830308
+FIRRM	CRISPRko	CAGTAGGCACTGCAGAATGC	AGG	0.4601	32	15	+	54	18	169837062
+FIRRM	CRISPRko	AACACAAACATGCTGATACA	AGG	0.5637	39	16	-	49	14	169829382
+FIRRM	CRISPRko	TAACTGTAAACAAGAATGGA	AGG	0.9413	81	17	-	9	9	169807883
+FIRRM	CRISPRko	TCATATGAACCACCTGACAT	TGG	0.2427	23	18	+	70	4	169800934
+FIRRM	CRISPRko	TTTTGCACTACGCTAAGGTA	AGG	0.05423	4	19	+	91	10	169821760
+FIRRM	CRISPRko	ACCAGTTCAGCAAACAGTGA	GGG	0.8077	77	20	-	21	14	169829435
+FIRRM	CRISPRko	AGCAAACAGTGAGGGATGAA	AGG	0.1146	16	21	-	85	14	169829427
+FIRRM	CRISPRko	TAAGTGGATCCAGTGTCACT	AGG	0.2822	36	22	-	65	12	169827126
+FIRRM	CRISPRko	GAATTAACCAGTCAAGCCAG	AGG	0.9117	89	23	+	14	5	169802668
+FIRRM	CRISPRko	AAGGAAATATGTTGCCACAG	AGG	1.01	99	24	-	5	18	169836978
+FIRRM	CRISPRko	GTAAGGCAAACATACAGGAC	AGG	0.8961	92	25	-	17	22	169849523
+FIRRM	CRISPRko	AAGCTCTAATTTAAGTAGCG	AGG	0.125	26	26	-	83	21	169847712
+FIRRM	CRISPRko	CTAAGGAAGTGCAAACCCTG	TGG	0.9352	101	27	+	10	13	169827800
+FIRRM	CRISPRko	AAGTACAGCAAGAATCAGAG	AGG	1.008	106	28	-	6	11	169823428
+FIRRM	CRISPRko	TAGGGAACAAACTGTCCATG	AGG	0.5913	68	29	+	46	18	169837033
+FIRRM	CRISPRko	TGGAAATCCAGACCACTCTA	AGG	-0.09848	9	30	+	105	5	169802708
+FIRRM	CRISPRko	AATTATATGAAGGACTGAGG	AGG	0.7188	84	31	-	31	6	169803269
+FIRRM	CRISPRko	GCTGAGCAGGAGTCGTACTT	TGG	-0.227	3	32	+	114	18	169837090
+FIRRM	CRISPRko	TAGCAGTAAGGCAAACATAC	AGG	0.1308	42	33	-	81	22	169849528
+FIRRM	CRISPRko	TGGCACCACCCCATCAGGCA	AGG	0.698	91	34	+	32	17	169832507
+FIRRM	CRISPRko	TTCCAGATAAAGTCATGCCC	TGG	0.7348	97	35	+	27	17	169832428
+FIRRM	CRISPRko	CAGCTCTTAGGAGCTCTCAC	AGG	0.4348	69	36	+	55	6	169803195
+FIRRM	CRISPRko	CCTGGCTGTCTGTGCACCAC	AGG	0.3254	63	37	-	62	13	169827805
+FIRRM	CRISPRko	ATGAAATGCATGGTCCATAC	TGG	-0.2804	6	38	-	119	8	169806046
+FIRRM	CRISPRko	TTACAGGGATTAAAGAGTAA	GGG	-0.215	15	39	+	112	14	169829350
+FIRRM	CRISPRko	ATTGCCAAACGAACATAGTC	AGG	-0.2223	17	40	-	113	21	169847735
+FIRRM	CRISPRko	CAAAATGATGGATTCCTGTG	TGG	0.6524	93	41	-	38	6	169803227
+FIRRM	CRISPRko	TACCTTAGCGTAGTGCAAAA	GGG	-0.3451	8	42	-	125	10	169821746
+FIRRM	CRISPRko	TACTGCTAGCTGACAGGAGT	TGG	0.003631	38	43	+	97	22	169849554
+FIRRM	CRISPRko	AGGTAATAGTATTATACCTT	TGG	0.09764	46	44	-	89	4	169800972
+FIRRM	CRISPRko	AAGCAGAAAATCTGCCTCTG	TGG	0.3627	82	45	+	58	18	169836975
+FIRRM	CRISPRko	CTGGTAGCATAAAGGCTTGG	AGG	0.2755	74	46	-	66	12	169827064
+FIRRM	CRISPRko	AATGTCAGGTGGTTCATATG	AGG	0.9125	129	47	-	13	4	169800921
+FIRRM	CRISPRko	TTATATGAAGGACTGAGGAG	GGG	0.9016	127	48	-	16	6	169803267
+FIRRM	CRISPRko	ATATCCTTCCTTGCCTGATG	GGG	0.6447	107	49	-	39	17	169832504
+FIRRM	CRISPRko	CTCCCTTTTGCACTACGCTA	AGG	-0.4703	5	50	+	142	10	169821755
+FIRRM	CRISPRko	TTATTATGGACTGGTGCTTA	AGG	-0.3294	24	51	-	123	9	169807803
+FIRRM	CRISPRko	AGGCTTGGAGGAAACTTGCT	GGG	0.5891	100	52	-	48	12	169827052
+FIRRM	CRISPRko	TACCAGTTCAGCAAACAGTG	AGG	0.907	136	53	-	15	14	169829436
+FIRRM	CRISPRko	GTTAATGGAACTGCTGGACA	TGG	0.6927	120	54	+	33	7	169804181
+FIRRM	CRISPRko	GATCACCTCTTTGTTAGCTA	TGG	-0.3588	28	55	+	126	15	169830314
+FIRRM	CRISPRko	ATAGTGAATCTGTGTATTCT	GGG	-0.4559	14	56	+	140	7	169804093
+FIRRM	CRISPRko	CTGGCTGTCTGTGCACCACA	GGG	0.7726	132	57	-	23	13	169827804
+FIRRM	CRISPRko	TTGGTGTGCTTTAGAAATCC	TGG	-0.1462	48	58	-	110	12	169827083
+FIRRM	CRISPRko	GCATTCTGCAGTGCCTACTG	TGG	0.562	109	59	-	50	18	169837048
+FIRRM	CRISPRko	ATGCATTTCATGCCAATACT	TGG	-0.3712	31	60	+	128	8	169806069
+FIRRM	CRISPRko	GCAAACATACAGGACAGGTT	GGG	0.6034	117	61	-	44	22	169849518
+FIRRM	CRISPRko	CTTTGTTAGCTATGGATGCA	TGG	-0.4268	27	62	+	134	15	169830322
+FIRRM	CRISPRko	GTCCATGAGGTCACCACAGT	AGG	-0.09508	58	63	+	104	18	169837046
+FIRRM	CRISPRko	TTACCTTAGCGTAGTGCAAA	AGG	-0.4911	18	64	-	144	10	169821747
+FIRRM	CRISPRko	AGGCGTTACCTCCTGAGCTT	AGG	-0.05311	62	65	+	101	18	169837014
+FIRRM	CRISPRko	TGAGTTGATAACATTCTCCA	GGG	1.286	161	66	-	2	17	169832434
+FIRRM	CRISPRko	TACAGGGATTAAAGAGTAAG	GGG	0.5164	111	67	+	52	14	169829351
+FIRRM	CRISPRko	AAATATTCCTTAGAGTGGTC	TGG	-0.437	30	68	-	135	5	169802704
+FIRRM	CRISPRko	TTGCCTTACTGCTAGCTGAC	AGG	-0.1406	57	69	+	109	22	169849548
+FIRRM	CRISPRko	AAGTCTGTTCCAATGTCAGG	TGG	0.6924	133	70	-	34	4	169800932
+FIRRM	CRISPRko	AAAGAGTAAGGGGAAAGCTG	AGG	0.7285	138	71	+	30	14	169829361
+FIRRM	CRISPRko	TGTGTATCTGAAGATACAGT	TGG	0.5898	121	72	-	47	11	169823455
+FIRRM	CRISPRko	TTTGGGAGAACTAGAATCTC	TGG	0.03227	76	73	+	94	18	169837108
+FIRRM	CRISPRko	TCCAAAACCACCTGCATGAA	AGG	-0.03522	71	74	-	99	12	169827172
+FIRRM	CRISPRko	TCAGCATATCTGTGCCACAC	AGG	0.6294	131	75	+	40	6	169803224
+FIRRM	CRISPRko	AGCGTAGTGCAAAAGGGAGT	TGG	1.038	170	76	-	4	10	169821740
+FIRRM	CRISPRko	TCCTTCCTTGCCTGATGGGG	TGG	0.5393	125	77	-	51	17	169832501
+FIRRM	CRISPRko	ATCCTGGTAGCATAAAGGCT	TGG	0.3835	119	78	-	57	12	169827067
+FIRRM	CRISPRko	AGGTGCTTTCCTAGTGACAC	TGG	-0.1063	70	79	+	107	12	169827128
+FIRRM	CRISPRko	TGTGGAAACTGCAGTTCACA	TGG	-0.05025	78	80	-	100	13	169827722
+FIRRM	CRISPRko	TGGTTGTCGTTTCTGAGACC	TGG	0.01372	85	81	-	96	13	169827823
+FIRRM	CRISPRko	GATGACACAGTCAGATGCAC	AGG	0.6544	144	82	+	37	9	169807925
+FIRRM	CRISPRko	GAAACTGCAGTTCACATGGC	AGG	0.1526	104	83	-	78	13	169827718
+FIRRM	CRISPRko	AAAGCAGTTAATGGAACTGC	TGG	0.052	90	84	+	92	7	169804175
+FIRRM	CRISPRko	TTTAATTATATGAAGGACTG	AGG	-0.06365	83	85	-	102	6	169803272
+FIRRM	CRISPRko	CAGACAATGGTGCAGCTCTT	AGG	-0.4748	43	86	+	143	6	169803183
+FIRRM	CRISPRko	TGTCACCATAGTGGCTCATC	TGG	-0.4259	54	87	+	133	16	169830743
+FIRRM	CRISPRko	CAGCAAGAATCAGAGAGGAA	AGG	0.7291	158	88	-	29	11	169823423
+FIRRM	CRISPRko	CTATGTTCGTTTGGCAATGT	TGG	-0.9313	7	89	+	181	21	169847751
+FIRRM	CRISPRko	GACGCTTCAACAGTATTGAT	AGG	0.3383	130	90	-	60	17	169832461
+FIRRM	CRISPRko	CTCAGGAGGTAACGCCTGGA	AGG	-0.4019	61	91	-	130	18	169836997
+FIRRM	CRISPRko	GCTTCCTGACTATGTTCGTT	TGG	-0.7699	25	92	+	169	21	169847742
+FIRRM	CRISPRko	CTTCATGGCACCACCCCATC	AGG	-1.028	11	93	+	187	17	169832502
+FIRRM	CRISPRko	TTTACAGGGATTAAAGAGTA	AGG	-0.5422	51	94	+	149	14	169829349
+FIRRM	CRISPRko	GATTTCTAAAGCACACCAAG	AGG	0.3263	142	95	+	61	12	169827098
+FIRRM	CRISPRko	GGCGTTACCTCCTGAGCTTA	GGG	-0.441	67	96	+	137	18	169837015
+FIRRM	CRISPRko	ACAGTTTGTTCCCTAAGCTC	AGG	0.7938	182	97	-	22	18	169837014
+FIRRM	CRISPRko	GTGGTTTTGGACAGTAAATT	AGG	-0.8207	29	98	+	175	12	169827195
+FIRRM	CRISPRko	AAGGCTTGGAGGAAACTTGC	TGG	-0.4154	73	99	-	132	12	169827053
+FIRRM	CRISPRko	AAGCACATTTGTGCATTGTA	AGG	-0.6518	47	100	+	159	6	169803305
+FIRRM	CRISPRko	GCACACCAAGAGGAAATAGC	AGG	-0.09987	102	101	+	106	12	169827108
+FIRRM	CRISPRko	ACTGGCTTTTTATTATGGAC	TGG	-0.7435	44	102	-	166	9	169807812
+FIRRM	CRISPRko	TTTTATACCTGAAGCTATCC	AGG	-1.205	22	103	+	194	21	169847799
+FIRRM	CRISPRko	ATGCATGGTGCTTCCTTGCT	CGG	0.2467	149	104	+	69	15	169830337
+FIRRM	CRISPRko	AATAGTGAATCTGTGTATTC	TGG	-0.958	34	105	+	184	7	169804092
+FIRRM	CRISPRko	CGAAGTTGTGAGTCAGCTTT	GGG	-1.584	12	106	+	207	19	169842514
+FIRRM	CRISPRko	GTTTGTTCCCTAAGCTCAGG	AGG	0.2075	147	107	-	72	18	169837011
+FIRRM	CRISPRko	AGGACTGTCAAGCCAAAATT	TGG	-0.6071	65	108	+	154	5	169802688
+FIRRM	CRISPRko	CTCCAAGCCTTTATGCTACC	AGG	-1.27	20	109	+	200	12	169827076
+FIRRM	CRISPRko	ATTTTGGCTTGACAGTCCTC	TGG	-0.7374	56	110	-	165	5	169802673
+FIRRM	CRISPRko	CAGCTAACTGTAAACAAGAA	TGG	-0.335	98	111	-	124	9	169807887
+FIRRM	CRISPRko	ATGAGTTGATAACATTCTCC	AGG	-0.1152	115	112	-	108	17	169832435
+FIRRM	CRISPRko	TGCACTTCCTTAGGCTGAGA	TGG	0.1058	139	113	-	87	13	169827779
+FIRRM	CRISPRko	CTGAATGTCTGTTGAACATA	AGG	-0.8447	50	114	-	177	20	169843692
+FIRRM	CRISPRko	CCTTCCTTATTCACAGACAA	TGG	-0.3247	105	115	+	122	6	169803170
+FIRRM	CRISPRko	CATCATTGTCATCTACTAAA	GGG	-0.8948	49	116	-	180	7	169804203
+FIRRM	CRISPRko	AGGCCTCCTTGAAAAGAGCC	TGG	-0.187	118	117	-	111	7	169804131
+FIRRM	CRISPRko	CTTTTCACAAGTGTTACCAA	AGG	-1.067	41	118	+	190	4	169800967
+FIRRM	CRISPRko	AGATCCTAAACTGATACTTC	AGG	-0.5402	86	119	+	148	20	169843765
+FIRRM	CRISPRko	AATGCAGGAAATGGCTGAGC	AGG	0.2731	167	120	+	67	18	169837077
+FIRRM	CRISPRko	TAAGCTGCCATCTCAGCCTA	AGG	0.1032	146	121	+	88	13	169827783
+FIRRM	CRISPRko	CACATTTCAGCCTTTCATGC	AGG	-0.6968	72	122	+	162	12	169827173
+FIRRM	CRISPRko	CCTGGAGAAGGTCTGAAACT	AGG	0.3254	172	123	-	63	7	169804113
+FIRRM	CRISPRko	ACCTGTACTCTTCTTCCCAG	GGG	0.866	219	124	-	19	25	169852825
+FIRRM	CRISPRko	ACTCCTGTCAGCTAGCAGTA	AGG	0.8819	222	125	-	18	22	169849540
+FIRRM	CRISPRko	TCGAAGTTGTGAGTCAGCTT	TGG	-0.8321	64	126	+	176	19	169842513
+FIRRM	CRISPRko	AGCGCTGCATACAATAGCAG	GGG	0.4628	188	127	+	53	25	169852860
+FIRRM	CRISPRko	GGCAAACATACAGGACAGGT	TGG	0.1195	157	128	-	84	22	169849519
+FIRRM	CRISPRko	GAGTAAGGGGAAAGCTGAGG	TGG	0.7617	217	129	+	24	14	169829364
+FIRRM	CRISPRko	TGACTGTAGAAGAAGCAAAG	AGG	0.7429	216	130	+	26	24	169851919
+FIRRM	CRISPRko	CTGAGCAGGAGTCGTACTTT	GGG	-1.052	53	131	+	189	18	169837091
+FIRRM	CRISPRko	CTGCTCTGTTATTTCCAGTA	TGG	-0.4504	103	132	+	139	8	169806043
+FIRRM	CRISPRko	ATTTCAGCCTTTCATGCAGG	TGG	-0.233	128	133	+	115	12	169827176
+FIRRM	CRISPRko	ACTGCTGGACATGGTTTGCA	TGG	0.08832	154	134	+	90	7	169804190
+FIRRM	CRISPRko	CCTAGTTTCAGACCTTCTCC	AGG	0.1271	163	135	+	82	7	169804124
+FIRRM	CRISPRko	CCTTGAAAAGAGCCTGGAGA	AGG	0.1343	166	136	-	80	7	169804125
+FIRRM	CRISPRko	TCTCCAGGCTCTTTTCAAGG	AGG	-0.2482	135	137	+	117	7	169804139
+FIRRM	CRISPRko	AATTATAAACTTCCAAGTAT	TGG	-0.8141	80	138	-	173	8	169806070
+FIRRM	CRISPRko	CTTACCTGAAGTATCAGTTT	AGG	-1.043	66	139	-	188	20	169843758
+FIRRM	CRISPRko	TGAATGAAAAATCCCAACAG	TGG	0.8193	235	140	-	20	20	169843725
+FIRRM	CRISPRko	TGACAGTCCTCTGGCTTGAC	TGG	-0.6604	95	141	-	161	5	169802664
+FIRRM	CRISPRko	TCTTCAGAACTGAGACACTG	TGG	0.7302	234	142	-	28	23	169850295
+FIRRM	CRISPRko	GCTGGTGAAGCTTTGGATAC	AGG	-0.8529	87	143	+	178	19	169842473
+FIRRM	CRISPRko	TGCCTACTGTGGTGACCTCA	TGG	-0.2921	145	144	-	120	18	169837037
+FIRRM	CRISPRko	TAGAAATCCTGGTAGCATAA	AGG	0.1868	191	145	-	74	12	169827072
+FIRRM	CRISPRko	TTTAATCCCTGTAAATGAAC	AGG	-0.8006	94	146	-	172	14	169829330
+FIRRM	CRISPRko	ACAACCAGAAGAAGACATTG	TGG	0.6818	230	147	-	36	13	169827740
+FIRRM	CRISPRko	TGAAACTGAAGCTGCCAAAG	TGG	0.6277	228	148	+	41	24	169851848
+FIRRM	CRISPRko	GCACTGCAGAATGCAGGAAA	TGG	0.03432	177	149	+	93	18	169837068
+FIRRM	CRISPRko	CACACAGGAATCCATCATTT	TGG	-1.21	75	150	+	195	6	169803239
+FIRRM	CRISPRko	AATATTACCTGGATAGCTTC	AGG	-0.07072	171	151	-	103	21	169847795
+FIRRM	CRISPRko	TGATATCCTTCCTTGCCTGA	TGG	-0.4079	143	152	-	131	17	169832506
+FIRRM	CRISPRko	TCCCCTGGGAAGAAGAGTAC	AGG	0.2194	205	153	+	71	25	169852835
+FIRRM	CRISPRko	TCCCTCACTGTTTGCTGAAC	TGG	-0.7029	113	154	+	163	14	169829445
+FIRRM	CRISPRko	TTGTAAAATATTCCTTAGAG	TGG	-0.5607	126	155	-	150	5	169802709
+FIRRM	CRISPRko	CTGGCTTTCAATGGAAATGG	AGG	0.1474	198	156	+	79	25	169852929
+FIRRM	CRISPRko	CTGCTCAAAACTGTAGAAAA	CGG	-0.2638	159	157	-	118	14	169829286
+FIRRM	CRISPRko	GATATCCTTCCTTGCCTGAT	GGG	-0.3988	150	158	-	129	17	169832505
+FIRRM	CRISPRko	TACAGTTTTGAGCAGTGTTC	TGG	-0.9555	96	159	+	183	14	169829305
+FIRRM	CRISPRko	AGCTCGTCAGGAGTTCCCCT	GGG	0.188	207	160	+	73	25	169852821
+FIRRM	CRISPRko	ATCCCAACAGTGGAAGTAAA	AGG	-0.6165	124	161	-	156	20	169843715
+FIRRM	CRISPRko	TGCAGTTATTCATTCTTTAT	TGG	-2.42	45	162	+	235	8	169806016
+FIRRM	CRISPRko	ATTATATGAAGGACTGAGGA	GGG	0.3947	224	163	-	56	6	169803268
+FIRRM	CRISPRko	TTTTAAACATTAAACAGGTA	AGG	-2.039	52	164	+	231	19	169842540
+FIRRM	CRISPRko	GACCTGTACTCTTCTTCCCA	GGG	0.2579	218	165	-	68	25	169852826
+FIRRM	CRISPRko	CTTGCAGTATGTAATTCTGC	TGG	-0.5729	134	166	+	152	19	169842455
+FIRRM	CRISPRko	TCATCATTGTCATCTACTAA	AGG	-1.252	88	167	-	198	7	169804204
+FIRRM	CRISPRko	GAACGTGTGAAACAGGAAAA	AGG	0.02462	193	168	+	95	24	169851870
+FIRRM	CRISPRko	TCTTCTTCTGGTTGTTGTCA	TGG	-0.3638	162	169	+	127	13	169827759
+FIRRM	CRISPRko	AAGCACCTGCTATTTCCTCT	TGG	-0.7457	122	170	-	167	12	169827102
+FIRRM	CRISPRko	AGAAAGTACCTTCTCCAGAA	AGG	0.1732	215	171	-	75	23	169850338
+FIRRM	CRISPRko	GGAGGCGCTCCAAGAAAGGA	TGG	0.1086	208	172	+	86	25	169852947
+FIRRM	CRISPRko	GTGGCAACATATTTCCTTCC	AGG	-0.9428	116	173	+	182	18	169836994
+FIRRM	CRISPRko	CCTGTGGTGCACAGACAGCC	AGG	-0.5237	155	174	+	145	13	169827816
+FIRRM	CRISPRko	GATATTTTGAATATGGTAAT	AGG	-1.163	112	175	+	191	7	169804236
+FIRRM	CRISPRko	GCATACAATAGCAGGGGCTT	TGG	-0.2981	184	176	+	121	25	169852866
+FIRRM	CRISPRko	GCCTTTCATGCAGGTGGTTT	TGG	-1.185	114	177	+	192	12	169827182
+FIRRM	CRISPRko	AGAAAGTCTGTTCCAATGTC	AGG	0.169	231	178	-	77	4	169800935
+FIRRM	CRISPRko	TGGTCTGGATTTCCAAATTT	TGG	-1.442	108	179	-	202	5	169802689
+FIRRM	CRISPRko	CACAGGGTTTGCACTTCCTT	AGG	-0.7772	141	180	-	170	13	169827788
+FIRRM	CRISPRko	TCTCTACCTGTTCATTTACA	GGG	-0.7313	148	181	+	164	14	169829335
+FIRRM	CRISPRko	TCAGCGCTGCATACAATAGC	AGG	-0.4406	178	182	+	136	25	169852858
+FIRRM	CRISPRko	TTCTCTTCAAAAGCAGTTAA	TGG	-1.2	123	183	+	193	7	169804166
+FIRRM	CRISPRko	AACGTTACATACATACTCTA	GGG	-0.4492	181	184	+	138	25	169852979
+FIRRM	CRISPRko	TGACCTGTACTCTTCTTCCC	AGG	0.002487	221	185	-	98	25	169852827
+FIRRM	CRISPRko	CAGCGCTGCATACAATAGCA	GGG	-0.5312	180	186	+	147	25	169852859
+FIRRM	CRISPRko	TAATTCTGCTGGTGAAGCTT	TGG	-0.5631	176	187	+	151	19	169842466
+FIRRM	CRISPRko	CTTCTTTTGGGGAAAATTTC	TGG	-1.817	110	188	-	220	18	169836944
+FIRRM	CRISPRko	TTCATGGTCCCTGGCAGACT	GGG	-0.4576	190	189	+	141	24	169851817
+FIRRM	CRISPRko	CTGTTTCACACGTTCCACTT	TGG	-0.6055	187	190	-	153	24	169851851
+FIRRM	CRISPRko	AGTTGTATCCTTTCTGGAGA	AGG	-0.2357	229	191	+	116	23	169850341
+FIRRM	CRISPRko	ATGTGCTTTTAATTATATGA	AGG	-1.633	137	192	-	213	6	169803279
+FIRRM	CRISPRko	CAAAGTGGAACGTGTGAAAC	AGG	-0.6244	194	193	+	157	24	169851863
+FIRRM	CRISPRko	GTTTCCACAATGTCTTCTTC	TGG	-1.257	152	194	+	199	13	169827747
+FIRRM	CRISPRko	TAACTGCTTTTGAAGAGAAT	AGG	-0.9912	173	195	-	185	7	169804151
+FIRRM	CRISPRko	CAGCCTTTTACTTCCACTGT	TGG	-1.585	151	196	+	208	20	169843723
+FIRRM	CRISPRko	TTATATCTTTGTGTTTCAAC	TGG	-1.509	156	197	-	204	9	169807830
+FIRRM	CRISPRko	CTCTCTACCTGTTCATTTAC	AGG	-1.821	140	198	+	222	14	169829334
+FIRRM	CRISPRko	AAATGGAGGCGCTCCAAGAA	AGG	-0.8152	189	199	+	174	25	169852943
+FIRRM	CRISPRko	TTATGCAAAAAGAGCTCGTC	AGG	-1.025	179	200	+	186	25	169852809
+FIRRM	CRISPRko	GAGCTCGTCAGGAGTTCCCC	TGG	-0.6133	214	201	+	155	25	169852820
+FIRRM	CRISPRko	TCTACAGTCACATTAGCAAA	GGG	-0.5241	223	202	-	146	24	169851895
+FIRRM	CRISPRko	TTCTACAGTCACATTAGCAA	AGG	-0.7777	200	203	-	171	24	169851896
+FIRRM	CRISPRko	CCTTCTCCAGGCTCTTTTCA	AGG	-1.649	160	204	+	214	7	169804136
+FIRRM	CRISPRko	CTCCAGGGCATGACTTTATC	TGG	-1.561	169	205	-	206	17	169832419
+FIRRM	CRISPRko	AGAGGTCATCTTTACAGGTA	TGG	-0.762	210	206	+	168	24	169851937
+FIRRM	CRISPRko	ATTGAAAGCCAGGCTGGAGC	AGG	-0.6531	220	207	-	160	25	169852907
+FIRRM	CRISPRko	GAGCTCTTTTTGCATAAGGC	TGG	-1.238	183	208	-	197	25	169852792
+FIRRM	CRISPRko	CTCTTTTATATTGCAGATAT	GGG	-1.968	153	209	+	230	16	169830697
+FIRRM	CRISPRko	ACTGAGTCACTACTCCAAAA	GGG	-0.6251	226	210	+	158	25	169852894
+FIRRM	CRISPRko	AAACGTTACATACATACTCT	AGG	-1.39	185	211	+	201	25	169852978
+FIRRM	CRISPRko	GGCTTTTTTAAACATTAAAC	AGG	-1.623	174	212	+	212	19	169842535
+FIRRM	CRISPRko	GAAGCGTCTCTTTTTCTTCA	TGG	-1.853	165	213	+	224	17	169832487
+FIRRM	CRISPRko	GCAGATTTTCTGCTTCTTTT	GGG	-1.947	164	214	-	228	18	169836956
+FIRRM	CRISPRko	CTGAATATTTTCCAAAATGA	TGG	-1.784	175	215	-	217	6	169803239
+FIRRM	CRISPRko	TTGGATTTTGTATCTTCTTT	AGG	-1.869	168	216	+	225	21	169847770
+FIRRM	CRISPRko	AACTGAGTCACTACTCCAAA	AGG	-1.62	196	217	+	211	25	169852893
+FIRRM	CRISPRko	AGCCTGGCTTTCAATGGAAA	TGG	-1.227	212	218	+	196	25	169852926
+FIRRM	CRISPRko	TGCTCCAGCCTGGCTTTCAA	TGG	-1.485	206	219	+	203	25	169852920
+FIRRM	CRISPRko	GAACAAAGTTGTATCCTTTC	TGG	-1.668	195	220	+	215	23	169850335
+FIRRM	CRISPRko	AAAAGGGTCCTGCTCCAGCC	TGG	-0.8786	233	221	+	179	25	169852910
+FIRRM	CRISPRko	CAATGATGATATTTTGAATA	TGG	-1.616	203	222	+	209	7	169804229
+FIRRM	CRISPRko	ATTTCCATTGAAAGCCAGGC	TGG	-1.538	209	223	-	205	25	169852913
+FIRRM	CRISPRko	TTAGCTTATCCATCCTTTCT	TGG	-1.928	192	224	-	227	25	169852945
+FIRRM	CRISPRko	AGGCTGGAGCAGGACCCTTT	TGG	-1.812	201	225	-	219	25	169852897
+FIRRM	CRISPRko	TGACGAGCTCTTTTTGCATA	AGG	-2.138	186	226	-	234	25	169852796
+FIRRM	CRISPRko	TTTCAACTGGCTTTTTATTA	TGG	-1.819	199	227	-	221	9	169807817
+FIRRM	CRISPRko	CTCCATTTCCATTGAAAGCC	AGG	-1.875	197	228	-	226	25	169852917
+FIRRM	CRISPRko	AGCAAAGAGGTCATCTTTAC	AGG	-1.96	202	229	+	229	24	169851932
+FIRRM	CRISPRko	CAGATTTTCTGCTTCTTTTG	GGG	-1.793	213	230	-	218	18	169836955
+FIRRM	CRISPRko	AACAGGAAAAAGGTATTTTC	TGG	-2.107	204	231	+	233	24	169851880
+FIRRM	CRISPRko	AGCCTTTTACTTCCACTGTT	GGG	-1.62	227	232	+	210	20	169843724
+FIRRM	CRISPRko	TCTCTTTTATATTGCAGATA	TGG	-1.717	225	233	+	216	16	169830696
+FIRRM	CRISPRko	GGCAGATTTTCTGCTTCTTT	TGG	-2.053	211	234	-	232	18	169836957
+FIRRM	CRISPRko	ACAGGAAAAAGGTATTTTCT	GGG	-1.825	232	235	+	223	24	169851881
+FGR	CRISPRko	AGCTTGGATTGAGTCAACAG	GGG	1.241	7	1	+	11	5	27621581
+FGR	CRISPRko	GCTGCCATGTCCACCAATTG	GGG	1.062	14	2	+	27	10	27614871
+FGR	CRISPRko	GCGGCTACTACATCACCACA	CGG	1.118	24	3	-	19	7	27616906
+FGR	CRISPRko	CTGCCCAGCATCCCTGTACG	AGG	0.8393	10	4	-	46	13	27613042
+FGR	CRISPRko	GTAGTTCATGCGTTCCATGT	AGG	1.046	37	5	+	29	11	27614562
+FGR	CRISPRko	AAGATCCGCAAACTGGACAT	GGG	1.076	46	6	-	24	7	27616928
+FGR	CRISPRko	GATGAGTACAACCCCTGCCA	AGG	1.108	55	7	-	20	11	27614435
+FGR	CRISPRko	CGAGTTGAACTGAACCCGTG	TGG	0.7891	19	8	+	56	7	27616902
+FGR	CRISPRko	GATGACCTCACCTTCACCAA	GGG	0.8977	33	9	-	42	4	27623075
+FGR	CRISPRko	AGGGGACTTCAGAAGCTACG	GGG	0.8336	28	10	-	47	3	27623835
+FGR	CRISPRko	CATGAATAAACGGGAAGTGT	TGG	0.6895	11	11	-	67	13	27613105
+FGR	CRISPRko	CTGACCGTGACACATGAACT	CGG	0.7547	21	12	+	60	9	27615446
+FGR	CRISPRko	GTGGAACGGCAGCACTAAGG	TGG	1.305	72	13	-	9	9	27615593
+FGR	CRISPRko	GGTTCAGTTCAACTCGGTGC	AGG	0.8085	38	14	-	52	7	27616884
+FGR	CRISPRko	CTGTTCCATGGCCTCGTACA	GGG	1.015	63	15	+	32	13	27613042
+FGR	CRISPRko	CGGCTACTACATCACCACAC	GGG	1.065	70	16	-	26	7	27616905
+FGR	CRISPRko	GAGCTGGTGCAGCACTACAT	GGG	0.7593	40	17	-	59	7	27616862
+FGR	CRISPRko	ACGAGGCCATGGAACAGACC	TGG	1.136	84	18	-	16	13	27613025
+FGR	CRISPRko	CAGACGCCAGGTCTGTTCCA	TGG	0.5595	12	19	+	91	13	27613030
+FGR	CRISPRko	TCCTCTCAGGCCATCAACCC	TGG	0.5191	4	20	-	100	3	27623732
+FGR	CRISPRko	AAAGCGAGACCACCAAAGGT	AGG	0.7056	43	21	-	64	6	27617194
+FGR	CRISPRko	GTGACCGAGTTCATGTGTCA	CGG	0.5849	26	22	-	86	9	27615439
+FGR	CRISPRko	GATGGTGAATCTGCCAAAGA	GGG	0.9319	76	23	+	38	12	27613306
+FGR	CRISPRko	TTGATGGGGAACTTGGAACC	TGG	0.748	56	24	+	62	12	27613349
+FGR	CRISPRko	AAGGGCCGAATCCCCTACCC	AGG	0.785	64	25	-	57	12	27613224
+FGR	CRISPRko	GTCATACAGGGCAATGAACA	GGG	0.9313	86	26	+	39	4	27623127
+FGR	CRISPRko	GGCGAGGCAAACCTGGGTAG	GGG	0.6916	61	27	+	66	12	27613224
+FGR	CRISPRko	GAGGCTCGGTCTCTCAGCTC	CGG	0.631	51	28	-	77	5	27621625
+FGR	CRISPRko	ACAGACCTGGCGTCTGGACC	CGG	0.6329	53	29	-	76	13	27613012
+FGR	CRISPRko	TGACTATGAGGCTCGAACTG	AGG	0.9885	94	30	-	35	4	27623097
+FGR	CRISPRko	GGCAGCCAACATCCTGGTTG	GGG	0.494	30	31	-	102	11	27614511
+FGR	CRISPRko	GCCATGCCCTCAGCTACCTG	GGG	1.096	113	32	+	21	11	27614584
+FGR	CRISPRko	TTTGAGGCTGCCCCAATTGG	TGG	0.2949	6	33	-	129	10	27614870
+FGR	CRISPRko	GAACGGCAGCACTAAGGTGG	CGG	1.31	129	34	-	8	9	27615590
+FGR	CRISPRko	ACAGCCTCCGAGCAGGCACG	TGG	0.9569	103	35	-	37	9	27615612
+FGR	CRISPRko	TGAACCACAGTACCAGCCCG	GGG	0.5456	47	36	-	94	13	27612931
+FGR	CRISPRko	CCTACAGTGAAGGTGACTGG	TGG	0.7944	88	37	-	55	5	27621648
+FGR	CRISPRko	GGTGCAGCACTACATGGGTG	AGG	0.5627	54	38	-	90	7	27616857
+FGR	CRISPRko	ATCCCAGTCCCGGATGGACA	GGG	0.8465	100	39	+	45	7	27616992
+FGR	CRISPRko	CACGTGGAACGGCAGCACTA	AGG	0.8131	96	40	-	50	9	27615596
+FGR	CRISPRko	CACACGGGTTCAGTTCAACT	CGG	0.2589	8	41	-	140	7	27616890
+FGR	CRISPRko	TAAACGGGAAGTGTTGGAAC	AGG	0.469	45	42	-	105	13	27613099
+FGR	CRISPRko	ATCATGAAGCCGCAGACGCT	GGG	0.1627	3	43	-	151	8	27615784
+FGR	CRISPRko	CCGGCTGCTTCGGGGATGTG	TGG	0.4943	57	44	-	101	8	27615699
+FGR	CRISPRko	CTGGCGAGGCAAACCTGGGT	AGG	0.6211	81	45	+	78	12	27613222
+FGR	CRISPRko	GCAGCTGTACGCCGTGGTGT	CGG	0.862	118	46	-	44	9	27615479
+FGR	CRISPRko	GCTGCCCCAATTGGTGGACA	TGG	0.2338	20	47	-	143	10	27614864
+FGR	CRISPRko	GACCTGGCGTCTGGACCCGG	AGG	1.274	155	48	-	10	13	27613009
+FGR	CRISPRko	CTACATTCACCGCGACCTGA	GGG	0.4697	65	49	-	104	11	27614532
+FGR	CRISPRko	TGGCCTCGTACAGGGATGCT	GGG	0.5425	75	50	+	95	13	27613050
+FGR	CRISPRko	ACTACATTCACCGCGACCTG	AGG	1.093	148	51	-	22	11	27614533
+FGR	CRISPRko	CAGCCGCAGCTCCATCACGC	TGG	0.8027	122	52	-	53	8	27615737
+FGR	CRISPRko	AAGCGAGACCACCAAAGGTA	GGG	0.7788	123	53	-	58	6	27617193
+FGR	CRISPRko	CAGCGTCTGCGGCTTCATGA	TGG	0.5825	95	54	+	87	8	27615797
+FGR	CRISPRko	GAAGCAGGGCACCTTGGCAG	GGG	1.198	169	55	+	14	11	27614435
+FGR	CRISPRko	GGACAGGGAGTAGGCACCTG	TGG	0.9147	143	56	+	41	7	27617007
+FGR	CRISPRko	TCAGCTTGGATTGAGTCAAC	AGG	0.1214	25	57	+	159	5	27621579
+FGR	CRISPRko	TGGCGAGGCAAACCTGGGTA	GGG	0.2614	48	58	+	139	12	27613223
+FGR	CRISPRko	CAGCTTGGATTGAGTCAACA	GGG	0.01986	9	59	+	178	5	27621580
+FGR	CRISPRko	TCGAGCCTCATAGTCATACA	GGG	0.9671	151	60	+	36	4	27623115
+FGR	CRISPRko	CTTGATAGTGGCACCATCAG	GGG	0.3869	73	61	-	114	3	27623705
+FGR	CRISPRko	GCTGTACGCCGTGGTGTCGG	AGG	1.132	175	62	-	17	9	27615476
+FGR	CRISPRko	CAGCCGGCGCTCCAGCGTGA	TGG	0.5471	99	63	+	93	8	27615737
+FGR	CRISPRko	CGAATGAGAAAGGCCCCCTG	GGG	0.6144	115	64	+	79	6	27617230
+FGR	CRISPRko	TCACCATCAAGTCAGACGTG	TGG	1.09	176	65	-	23	12	27613280
+FGR	CRISPRko	AGCATCCCTGTACGAGGCCA	TGG	0.3538	82	66	-	119	13	27613036
+FGR	CRISPRko	CGGGACTGGGATCAGACCAG	AGG	0.5505	112	67	-	92	7	27616970
+FGR	CRISPRko	GCTGGTGCAGCTGTACGCCG	TGG	1.048	177	68	-	28	9	27615485
+FGR	CRISPRko	GAATGAGAAAGGCCCCCTGG	GGG	0.9914	172	69	+	34	6	27617231
+FGR	CRISPRko	CGAGTTCATGTGTCACGGTC	AGG	-0.1493	1	70	-	208	9	27615434
+FGR	CRISPRko	CGTGGCACCACCCCTACCTT	TGG	0.07462	44	71	+	168	6	27617193
+FGR	CRISPRko	CTGGTACTGTGGTTCAGCGG	AGG	0.2301	71	72	+	144	13	27612949
+FGR	CRISPRko	TCTGTTCCATGGCCTCGTAC	AGG	0.3446	93	73	+	122	13	27613041
+FGR	CRISPRko	GTCCCGGATGGACAGGGAGT	AGG	0.8322	170	74	+	48	7	27616998
+FGR	CRISPRko	TGGTCACTTACGTATTGTTC	AGG	-0.2006	2	75	+	217	4	27623048
+FGR	CRISPRko	TGTTGGCTGCCCTCAGGTCG	CGG	1.238	209	76	+	12	11	27614534
+FGR	CRISPRko	ATCCGCAAACTGGACATGGG	CGG	0.6763	152	77	-	69	7	27616925
+FGR	CRISPRko	CCAGGATGTTGGCTGCCCTC	AGG	0.9183	183	78	+	40	11	27614528
+FGR	CRISPRko	CCACACATCCCCGAAGCAGC	CGG	0.4556	114	79	+	110	8	27615710
+FGR	CRISPRko	CGGGAAAGCGAGACCACCAA	AGG	0.3369	101	80	-	123	6	27617198
+FGR	CRISPRko	GCTGCTGCGGCACGACAAGC	TGG	-0.06437	36	81	-	190	9	27615503
+FGR	CRISPRko	CATGGAACAGACCTGGCGTC	TGG	0.4629	121	82	-	107	13	27613018
+FGR	CRISPRko	CTCCGAGCAGGCACGTGGAA	CGG	0.002057	50	83	-	179	9	27615607
+FGR	CRISPRko	TGGCTTGGCGCGTCTCATCA	AGG	-0.07207	39	84	-	193	11	27614460
+FGR	CRISPRko	GAAGGACTGCAGGTACTCGA	AGG	1.223	221	85	+	13	13	27612988
+FGR	CRISPRko	TGAAGCAGGGCACCTTGGCA	GGG	0.6414	168	86	+	73	11	27614434
+FGR	CRISPRko	CTGCATTCCCAGCAACTACG	TGG	0.3747	125	87	-	116	5	27621593
+FGR	CRISPRko	ATGGTCCCCCAGGTAGCTGA	GGG	1.071	217	88	-	25	11	27614579
+FGR	CRISPRko	TCCTTCAGGCATGAATAAAC	GGG	-0.04814	60	89	-	186	13	27613114
+FGR	CRISPRko	GGTCTGATCCCAGTCCCGGA	TGG	0.6097	166	90	+	81	7	27616986
+FGR	CRISPRko	CTACAGTGAAGGTGACTGGT	GGG	0.6401	173	91	-	74	5	27621647
+FGR	CRISPRko	TACCACATGCCGTGCCCTCC	AGG	0.4308	137	92	-	112	13	27613065
+FGR	CRISPRko	ACAGCCCGTCATTCACCTCT	AGG	0.2024	102	93	+	148	8	27615844
+FGR	CRISPRko	TGCCACTATCAAGGAAGCCA	GGG	0.7051	185	94	+	65	3	27623726
+FGR	CRISPRko	TGCTTCGGGGATGTGTGGCT	GGG	0.3884	138	95	-	113	8	27615694
+FGR	CRISPRko	AAGGGGACTTCAGAAGCTAC	GGG	-0.2167	32	96	-	219	3	27623836
+FGR	CRISPRko	CAGAACACACAGCCCATTCC	AGG	-0.286	27	97	+	224	3	27623917
+FGR	CRISPRko	CCTTGATAGTGGCACCATCA	GGG	-0.2007	35	98	-	218	3	27623706
+FGR	CRISPRko	TGATGGTGAATCTGCCAAAG	AGG	0.5765	167	99	+	88	12	27613305
+FGR	CRISPRko	TGCGGCTTCATGATGGTGCA	GGG	0.1716	106	100	+	149	8	27615804
+FGR	CRISPRko	CTCACCTGACACACCCCTGA	TGG	-0.1307	52	101	+	203	3	27623703
+FGR	CRISPRko	GGCACCACCCCTACCTTTGG	TGG	0.3735	139	102	+	117	6	27617196
+FGR	CRISPRko	GTACCTGCAGTCCTTCCTGG	AGG	1.742	256	103	-	1	13	27612970
+FGR	CRISPRko	CAGCCCGTCATTCACCTCTA	GGG	-0.4042	18	104	+	239	8	27615845
+FGR	CRISPRko	GCCAAAGAGGGCAGCTTCTG	GGG	0.5857	174	105	+	85	12	27613318
+FGR	CRISPRko	TTCACCAGGCAACCCCCAGG	GGG	0.638	184	106	-	75	6	27617232
+FGR	CRISPRko	GCTGGGCACCGGCTGCTTCG	GGG	1.148	245	107	-	15	8	27615707
+FGR	CRISPRko	GTTCTGCAAGAAATTGGAGC	CGG	0.2833	130	108	-	133	3	27623889
+FGR	CRISPRko	GAGAGGCAGCTGCTTTCACC	AGG	0.1364	108	109	-	156	6	27617246
+FGR	CRISPRko	ATGCTTCACATGATCGCCTC	TGG	-0.52	13	110	+	255	7	27616965
+FGR	CRISPRko	ATGGCCTCGTACAGGGATGC	TGG	-0.09879	69	111	+	200	13	27613049
+FGR	CRISPRko	CTTACGTATTGTTCAGGATG	TGG	0.08339	104	112	+	166	4	27623054
+FGR	CRISPRko	GATGGGCTCCTCCGACACCA	CGG	1.435	268	113	+	4	9	27615479
+FGR	CRISPRko	CCTCCCCTAGAGGTGAATGA	CGG	-0.07489	78	114	-	194	8	27615838
+FGR	CRISPRko	AGCCTGGAGGGCACGGCATG	TGG	0.2429	131	115	+	142	13	27613074
+FGR	CRISPRko	GCATTACAAGATCCGCAAAC	TGG	-0.3369	41	116	-	232	7	27616935
+FGR	CRISPRko	CTCACTGAGCTCATCACCAA	GGG	0.2772	140	117	-	135	12	27613242
+FGR	CRISPRko	GCTCACTGAGCTCATCACCA	AGG	0.8796	232	118	-	43	12	27613243
+FGR	CRISPRko	GAACTCGGTCACGATGTAGA	TGG	0.4505	164	119	+	111	9	27615461
+FGR	CRISPRko	GGAGCTGGTGCAGCACTACA	TGG	0.5926	191	120	-	84	7	27616863
+FGR	CRISPRko	GTGTTGGAACAGGTGGAGCA	GGG	1.377	271	121	-	5	13	27613089
+FGR	CRISPRko	AACAGGGGCCACGTAGTTGC	TGG	-0.09866	79	122	+	199	5	27621596
+FGR	CRISPRko	CAGTGAAGGTGACTGGTGGG	AGG	0.6613	208	123	-	70	5	27621644
+FGR	CRISPRko	TCGAAGGTAGGCCTCTCCTC	CGG	0.3505	159	124	+	121	13	27613004
+FGR	CRISPRko	GGTGGTACTTTGGAAAGATT	GGG	-0.0868	85	125	-	196	6	27617281
+FGR	CRISPRko	GACAGGCTATGTCTGATCCC	CGG	-0.5131	29	126	+	253	13	27612925
+FGR	CRISPRko	ACGGGAAGTGTTGGAACAGG	TGG	0.7121	219	127	-	63	13	27613096
+FGR	CRISPRko	TTGGGAGAAAGGATGCAGAG	AGG	1.317	279	128	-	7	6	27617263
+FGR	CRISPRko	GGGCAGCCAACATCCTGGTT	GGG	-0.06118	98	129	-	189	11	27614512
+FGR	CRISPRko	TTTCCCAACAGGTGGTACTT	TGG	-0.7244	15	130	-	272	6	27617291
+FGR	CRISPRko	CAAGATCGCAGACTTTGGCT	TGG	0.4559	179	131	-	109	11	27614475
+FGR	CRISPRko	GAAGGGGACTTCAGAAGCTA	CGG	-0.002412	109	132	-	180	3	27623837
+FGR	CRISPRko	TGGGTAGGGGATTCGGCCCT	TGG	-0.2764	67	133	+	223	12	27613237
+FGR	CRISPRko	GGATGACCTCACCTTCACCA	AGG	1.491	287	134	-	3	4	27623076
+FGR	CRISPRko	GCCCTGACTCTGCAGGGATT	GGG	-0.3203	59	135	-	231	4	27623143
+FGR	CRISPRko	GGATTTGAGGCTGCCCCAAT	TGG	-0.925	5	136	-	291	10	27614873
+FGR	CRISPRko	TGGTGCAGGGCGCGATGAGC	AGG	0.4616	189	137	+	108	8	27615817
+FGR	CRISPRko	CCGAATGAGAAAGGCCCCCT	GGG	-0.4071	58	138	+	240	6	27617229
+FGR	CRISPRko	AGTCATACAGGGCAATGAAC	AGG	0.05486	126	139	+	172	4	27623126
+FGR	CRISPRko	CTCAGTGAGCAGGATCCCAA	AGG	0.06232	128	140	+	171	12	27613267
+FGR	CRISPRko	CAAGATCCGCAAACTGGACA	TGG	-0.009147	119	141	-	181	7	27616929
+FGR	CRISPRko	ACGGGGCAGCAGACCACTAT	GGG	-0.842	17	142	-	283	3	27623818
+FGR	CRISPRko	GTGCCTACAGTGAAGGTGAC	TGG	0.06325	132	143	-	169	5	27621651
+FGR	CRISPRko	TCATGACCTGCGCCTCCTCC	AGG	0.685	234	144	+	68	9	27615538
+FGR	CRISPRko	GGAGCTGCGGCTGATCTCCC	AGG	0.4744	199	145	+	103	8	27615758
+FGR	CRISPRko	GGTGAAGTAGTCCTCCAGGA	AGG	1.637	301	146	+	2	13	27612970
+FGR	CRISPRko	ACGCCAGCCGCTCCCCAACC	AGG	0.5248	204	147	+	99	11	27614510
+FGR	CRISPRko	GGCCTGACCCCACTAAGGCC	CGG	-0.08602	110	148	-	195	3	27623797
+FGR	CRISPRko	CCCGAATGAGAAAGGCCCCC	TGG	0.2301	161	149	+	145	6	27617228
+FGR	CRISPRko	ATGCAGGCCGGGCCTTAGTG	GGG	0.1436	154	150	+	152	3	27623801
+FGR	CRISPRko	CCCTGATGGTGCCACTATCA	AGG	-0.6127	42	151	+	265	3	27623717
+FGR	CRISPRko	GTCCCCGAAGGCCTTCCTGG	AGG	1.348	302	152	-	6	9	27615542
+FGR	CRISPRko	GCGTGCAAGATCGCAGACTT	TGG	0.1198	149	153	-	160	11	27614480
+FGR	CRISPRko	GGCTGCCATGTCCACCAATT	GGG	-0.9446	16	154	+	293	10	27614870
+FGR	CRISPRko	CTCCCCTAGAGGTGAATGAC	GGG	-0.01895	127	155	-	182	8	27615837
+FGR	CRISPRko	ATTGGTGGACATGGCAGCCC	AGG	0.661	239	156	-	71	10	27614855
+FGR	CRISPRko	ATCACGCTGGAGCGCCGGCT	GGG	-0.4584	62	157	-	248	8	27615724
+FGR	CRISPRko	GCCACGGCCAAGGAGGATGC	TGG	0.2958	182	158	-	128	3	27623864
+FGR	CRISPRko	GGCCGGGCCTTAGTGGGGTC	AGG	-0.8719	23	159	+	288	3	27623806
+FGR	CRISPRko	ACAGGCTATGTCTGATCCCC	GGG	-0.1035	111	160	+	201	13	27612926
+FGR	CRISPRko	ACTCCCTGTCCATCCGGGAC	TGG	-0.4508	66	161	-	247	7	27616984
+FGR	CRISPRko	AACTCGGTCACGATGTAGAT	GGG	-0.08787	117	162	+	197	9	27615462
+FGR	CRISPRko	CGAAGGTAGGCCTCTCCTCC	GGG	0.2168	171	163	+	146	13	27613005
+FGR	CRISPRko	TATGGTCCCCCAGGTAGCTG	AGG	0.5996	236	164	-	82	11	27614580
+FGR	CRISPRko	GACCTGCGCCTCCTCCAGGA	AGG	1.041	290	165	+	30	9	27615542
+FGR	CRISPRko	CCCGAAGGCCTTCCTGGAGG	AGG	1.119	304	166	-	18	9	27615539
+FGR	CRISPRko	CATCATGAAGCCGCAGACGC	TGG	-0.1311	120	167	-	204	8	27615785
+FGR	CRISPRko	TCCTTGATAGTGGCACCATC	AGG	-1.256	22	168	-	302	3	27623707
+FGR	CRISPRko	CTCCCTGTCCATCCGGGACT	GGG	-0.493	74	169	-	252	7	27616983
+FGR	CRISPRko	GGGCTGGTACTGTGGTTCAG	CGG	0.08894	163	170	+	164	13	27612946
+FGR	CRISPRko	CTCGCTTTCCCGAATGAGAA	AGG	-0.4818	80	171	+	249	6	27617220
+FGR	CRISPRko	CTATGGGCCTGACCCCACTA	AGG	-0.1349	124	172	-	205	3	27623802
+FGR	CRISPRko	CCGCTCCCCAACCAGGATGT	TGG	0.8318	281	173	+	49	11	27614517
+FGR	CRISPRko	CTTTCACCAGGCAACCCCCA	GGG	0.03005	156	174	-	175	6	27617234
+FGR	CRISPRko	GAACTTCTCGCCCTTGGTGA	AGG	0.2766	195	175	+	136	4	27623076
+FGR	CRISPRko	GATGCAGGCCGGGCCTTAGT	GGG	-0.841	49	176	+	282	3	27623800
+FGR	CRISPRko	ATCCTTCAGGCATGAATAAA	CGG	-1.039	34	177	-	298	13	27613115
+FGR	CRISPRko	CATTGCCCTGTATGACTATG	AGG	0.03291	158	178	-	174	4	27623109
+FGR	CRISPRko	TTCGAGCCTCATAGTCATAC	AGG	-0.3641	97	179	+	235	4	27623114
+FGR	CRISPRko	GGCGTCTGGACCCGGAGGAG	AGG	0.3144	207	180	-	126	13	27613004
+FGR	CRISPRko	CGGGGATGTGTGGCTGGGTA	CGG	-0.4116	91	181	-	242	8	27615689
+FGR	CRISPRko	CTGTTCTGTGCCTACAGTGA	AGG	0.2892	202	182	-	131	5	27621658
+FGR	CRISPRko	GTGCCACTATCAAGGAAGCC	AGG	-1.297	31	183	+	304	3	27623725
+FGR	CRISPRko	CTGGGGCTGTCCACTTGATG	GGG	1.033	305	184	+	31	12	27613335
+FGR	CRISPRko	GCCAGCATCCTCCTTGGCCG	TGG	0.3527	216	185	+	120	3	27623874
+FGR	CRISPRko	CAGGAAGGCCTTCGGGGACA	TGG	0.3132	210	186	+	127	9	27615557
+FGR	CRISPRko	CTGCAAGAAATTGGAGCCGG	TGG	0.02057	160	187	-	177	3	27623886
+FGR	CRISPRko	CTGGAGCGCCGGCTGGGCAC	CGG	0.8129	289	188	-	51	8	27615718
+FGR	CRISPRko	AGGTGGTACTTTGGAAAGAT	TGG	-0.489	89	189	-	251	6	27617282
+FGR	CRISPRko	GTTGCTGTAGTTGGGGATGT	GGG	0.141	187	190	+	153	3	27623769
+FGR	CRISPRko	AGTCAGACGTGTGGTCCTTT	GGG	-0.7313	68	191	-	273	12	27613271
+FGR	CRISPRko	GTTCCAAGTTCCCCATCAAG	TGG	1.011	308	192	-	33	12	27613334
+FGR	CRISPRko	AGTGTTGGAACAGGTGGAGC	AGG	0.5332	244	193	-	98	13	27613090
+FGR	CRISPRko	TGCCTACTCCCTGTCCATCC	GGG	0.2714	206	194	-	137	7	27616989
+FGR	CRISPRko	GATCCCAGTCCCGGATGGAC	AGG	0.08804	178	195	+	165	7	27616991
+FGR	CRISPRko	GGATTTTCTCAAGAACCCAG	AGG	-0.09213	146	196	-	198	10	27614900
+FGR	CRISPRko	CCTGAGGGCAGCCAACATCC	TGG	0.6134	267	197	-	80	11	27614517
+FGR	CRISPRko	TCTCGCCCTTGGTGAAGGTG	AGG	0.326	222	198	+	125	4	27623081
+FGR	CRISPRko	AGTCCTCCAGGAAGGACTGC	AGG	0.4668	242	199	+	106	13	27612978
+FGR	CRISPRko	ACAGGGGCCACGTAGTTGCT	GGG	0.8023	295	200	+	54	5	27621597
+FGR	CRISPRko	CCCTGACTCTGCAGGGATTG	GGG	-0.5756	87	201	-	262	4	27623142
+FGR	CRISPRko	GCAGACGCTGGGCCTGGCCA	AGG	0.5368	253	202	-	97	8	27615773
+FGR	CRISPRko	AGGGATGCTGGGCAGCCTGG	AGG	0.598	270	203	+	83	13	27613061
+FGR	CRISPRko	GCTGGGCAGCCTGGAGGGCA	CGG	0.5416	257	204	+	96	13	27613067
+FGR	CRISPRko	GACTGCAGGTACTCGAAGGT	AGG	0.7535	294	205	+	61	13	27612992
+FGR	CRISPRko	GGGATGCTGGGCAGCCTGGA	GGG	0.659	286	206	+	72	13	27613062
+FGR	CRISPRko	GCAAACCTGGGTAGGGGATT	CGG	-0.7533	83	207	+	275	12	27613230
+FGR	CRISPRko	TGGTGATGAGCTCAGTGAGC	AGG	0.2087	212	208	+	147	12	27613257
+FGR	CRISPRko	ATTGGGGCAGCCTCAAATCC	TGG	-0.2958	134	209	+	226	10	27614887
+FGR	CRISPRko	CGCAGGTCATGAAGCTGCTG	CGG	0.3775	247	210	-	115	9	27615516
+FGR	CRISPRko	GAAAATCCAGCAAGCTGCCT	GGG	-0.5346	105	211	+	259	10	27614926
+FGR	CRISPRko	AGGAGAAGTTGCTGTAGTTG	GGG	0.114	203	212	+	161	3	27623762
+FGR	CRISPRko	CGGAGGTGAAGTAGTCCTCC	AGG	0.06294	196	213	+	170	13	27612966
+FGR	CRISPRko	GTGCCTACTCCCTGTCCATC	CGG	-0.3194	136	214	-	230	7	27616990
+FGR	CRISPRko	GTTCAACTCGGTGCAGGAGC	TGG	0.1082	205	215	-	162	7	27616878
+FGR	CRISPRko	CTTTGGAAAGATTGGGAGAA	AGG	-0.7592	92	216	-	276	6	27617274
+FGR	CRISPRko	GAAATTGGAGCCGGTGGCCA	CGG	-0.5691	107	217	-	261	3	27623880
+FGR	CRISPRko	GGACCACACGTCTGACTTGA	TGG	-0.1949	153	218	+	216	12	27613288
+FGR	CRISPRko	CTGAACCACAGTACCAGCCC	GGG	-0.3109	144	219	-	228	13	27612932
+FGR	CRISPRko	GCCGGGCACCATGTCCCCGA	AGG	-0.07068	180	220	-	192	9	27615554
+FGR	CRISPRko	TTGGCCAGGCCCAGCGTCTG	CGG	0.5723	284	221	+	89	8	27615786
+FGR	CRISPRko	GCCTCAAATCCTGGCCCTCT	GGG	-0.8498	90	222	+	284	10	27614896
+FGR	CRISPRko	TCCCTACTTACTCTTCAGCT	TGG	-0.02019	197	223	+	183	5	27621565
+FGR	CRISPRko	CCAGGGGGCCTTTCTCATTC	GGG	-1.436	77	224	-	306	6	27617217
+FGR	CRISPRko	GCGGTGAAGACGCTGAAGCC	GGG	0.1268	226	225	-	158	9	27615571
+FGR	CRISPRko	ATGTGGGCAAATGAGGATGC	AGG	0.2892	262	226	+	130	3	27623785
+FGR	CRISPRko	GCTTTCACCAGGCAACCCCC	AGG	0.3274	269	227	-	124	6	27617235
+FGR	CRISPRko	AAGGTGACTGGTGGGAGGCT	CGG	-0.1431	188	228	-	207	5	27621639
+FGR	CRISPRko	GGCCATGCCCTCAGCTACCT	GGG	-0.5259	141	229	+	257	11	27614583
+FGR	CRISPRko	GCTATGTCTGATCCCCGGGC	TGG	-0.5263	142	230	+	258	13	27612930
+FGR	CRISPRko	GATGTGGAACTTCTCGCCCT	TGG	0.2545	259	231	+	141	4	27623070
+FGR	CRISPRko	GCCAGGGTTGATGGCCTGAG	AGG	0.2799	266	232	+	134	3	27623742
+FGR	CRISPRko	GGAGGATGCTGGCCTGGAAG	GGG	0.1665	250	233	-	150	3	27623853
+FGR	CRISPRko	GAGGAGAAGTTGCTGTAGTT	GGG	-0.616	135	234	+	266	3	27623761
+FGR	CRISPRko	AGCCGCCCATGTCCAGTTTG	CGG	-0.3799	165	235	+	237	7	27616934
+FGR	CRISPRko	GGCGGTGAAGACGCTGAAGC	CGG	0.1079	240	236	-	163	9	27615572
+FGR	CRISPRko	TTTCACCAGGCAACCCCCAG	GGG	-0.1502	194	237	-	209	6	27617233
+FGR	CRISPRko	GCCCCAGAAGCTGCCCTCTT	TGG	-0.9429	116	238	-	292	12	27613308
+FGR	CRISPRko	CTGCGGCTTCATGATGGTGC	AGG	-0.04231	225	239	+	184	8	27615803
+FGR	CRISPRko	AGCCTCAAATCCTGGCCCTC	TGG	-0.6078	147	240	+	264	10	27614895
+FGR	CRISPRko	GGCCTTCCTGGAGGAGGCGC	AGG	0.1368	258	241	-	155	9	27615533
+FGR	CRISPRko	GGGCCTGGCCAAGGACGCCT	GGG	-0.35	181	242	-	234	8	27615764
+FGR	CRISPRko	AACCCTGGCTTCCTTGATAG	TGG	0.1279	260	243	-	157	3	27623717
+FGR	CRISPRko	GGATGCAGGCCGGGCCTTAG	TGG	-0.6687	150	244	+	268	3	27623799
+FGR	CRISPRko	CATCACGCTGGAGCGCCGGC	TGG	-0.3135	190	245	-	229	8	27615725
+FGR	CRISPRko	TAGTGGGGTCAGGCCCATAG	TGG	0.3639	303	246	+	118	3	27623816
+FGR	CRISPRko	TCCTCCTTGGCCGTGGCCAC	CGG	-0.247	200	247	+	222	3	27623881
+FGR	CRISPRko	TTCTGGGGCTGTCCACTTGA	TGG	-0.1678	211	248	+	214	12	27613333
+FGR	CRISPRko	CTCCTCCAGGAAGGCCTTCG	GGG	-0.04449	243	249	+	185	9	27615551
+FGR	CRISPRko	CTGCCAAAGAGGGCAGCTTC	TGG	-1.007	133	250	+	296	12	27613316
+FGR	CRISPRko	AGGGCAGCCAACATCCTGGT	TGG	0.2849	297	251	-	132	11	27614513
+FGR	CRISPRko	GCCTCCTCCAGGAAGGCCTT	CGG	-0.1543	218	252	+	211	9	27615549
+FGR	CRISPRko	GTTGGGGATGTGGGCAAATG	AGG	0.1378	277	253	+	154	3	27623778
+FGR	CRISPRko	TGATCCCCGGGCTGGTACTG	TGG	0.2671	300	254	+	138	13	27612938
+FGR	CRISPRko	CCAACATCCTGGTTGGGGAG	CGG	-0.2306	220	255	-	220	11	27614506
+FGR	CRISPRko	GCTGAACCACAGTACCAGCC	CGG	-1.125	145	256	-	301	13	27612933
+FGR	CRISPRko	GGAGCCGGTGGCCACGGCCA	AGG	-0.06537	255	257	-	191	3	27623874
+FGR	CRISPRko	AGCCCTGACTCTGCAGGGAT	TGG	-0.8613	162	258	-	285	4	27623144
+FGR	CRISPRko	CTACAGCAACTTCTCCTCTC	AGG	-0.5835	186	259	-	263	3	27623745
+FGR	CRISPRko	AGTTGCTGTAGTTGGGGATG	TGG	-0.2337	229	260	+	221	3	27623768
+FGR	CRISPRko	CTCTGGTCTGATCCCAGTCC	CGG	0.05226	278	261	+	173	7	27616982
+FGR	CRISPRko	GCCGGTGGCCACGGCCAAGG	AGG	-0.1588	241	262	-	212	3	27623871
+FGR	CRISPRko	AGCTGAGGGCATGGCCTACA	TGG	-0.04863	273	263	-	187	11	27614565
+FGR	CRISPRko	TGGGCCTGGCCAAGGACGCC	TGG	-0.7004	192	264	-	270	8	27615765
+FGR	CRISPRko	CGAAGCAGCCGGTGCCCAGC	CGG	-0.1783	248	265	+	215	8	27615721
+FGR	CRISPRko	CCCAGGGGGCCTTTCTCATT	CGG	-1.776	157	266	-	308	6	27617218
+FGR	CRISPRko	GGCTGGGCACCGGCTGCTTC	GGG	-0.7351	193	267	-	274	8	27615708
+FGR	CRISPRko	GCTCCAGCGTGATGGAGCTG	CGG	-0.4241	223	268	+	244	8	27615745
+FGR	CRISPRko	CGAGTACCTGCAGTCCTTCC	TGG	-0.05298	283	269	-	188	13	27612973
+FGR	CRISPRko	TACGGGGCAGCAGACCACTA	TGG	0.07913	309	270	-	167	3	27623819
+FGR	CRISPRko	CATGTCCCCGAAGGCCTTCC	TGG	-0.4356	233	271	-	246	9	27615545
+FGR	CRISPRko	GCTCCATCACGCTGGAGCGC	CGG	-0.4861	230	272	-	250	8	27615729
+FGR	CRISPRko	TCTGGGGCTGTCCACTTGAT	GGG	0.02854	307	273	+	176	12	27613334
+FGR	CRISPRko	TCTCTCAGCTCCGGAAAAAC	TGG	-0.1521	274	274	-	210	5	27621616
+FGR	CRISPRko	AAGTCAGACGTGTGGTCCTT	TGG	-0.5189	237	275	-	254	12	27613272
+FGR	CRISPRko	AAGGAGGATGCTGGCCTGGA	AGG	-0.1373	285	276	-	206	3	27623855
+FGR	CRISPRko	GGGCAAATGAGGATGCAGGC	CGG	-0.3094	265	277	+	227	3	27623789
+FGR	CRISPRko	CGGCTGGGCACCGGCTGCTT	CGG	-0.7761	215	278	-	278	8	27615709
+FGR	CRISPRko	CTGCTTCGGGGATGTGTGGC	TGG	-1.019	198	279	-	297	8	27615695
+FGR	CRISPRko	AGAGGAGAAGTTGCTGTAGT	TGG	-0.6778	228	280	+	269	3	27623760
+FGR	CRISPRko	AGGAGGATGCTGGCCTGGAA	GGG	-0.6255	235	281	-	267	3	27623854
+FGR	CRISPRko	TCTCAAGAACCCAGAGGGCC	AGG	-0.9112	214	282	-	290	10	27614894
+FGR	CRISPRko	AGAAAATCCAGCAAGCTGCC	TGG	-0.1628	292	283	+	213	10	27614925
+FGR	CRISPRko	ACCCAGAGGGCCAGGATTTG	AGG	-0.2927	282	284	-	225	10	27614886
+FGR	CRISPRko	TGTCCACTTGATGGGGAACT	TGG	-0.1174	306	285	+	202	12	27613342
+FGR	CRISPRko	CATCCTGGTTGGGGAGCGGC	TGG	-0.5244	252	286	-	256	11	27614502
+FGR	CRISPRko	CTCCTCCGGGTCCAGACGCC	AGG	-0.4247	264	287	+	245	13	27613018
+FGR	CRISPRko	GGGAATGCAGCCAGTTTTTC	CGG	-1.854	201	288	+	309	5	27621617
+FGR	CRISPRko	ATCAAGGAAGCCAGGGTTGA	TGG	-0.4021	275	289	+	238	3	27623733
+FGR	CRISPRko	CTTCTTCCCAGGCAGCTTGC	TGG	-0.8712	227	290	-	287	10	27614921
+FGR	CRISPRko	GTGAAGCAGGGCACCTTGGC	AGG	-0.4099	276	291	+	241	11	27614433
+FGR	CRISPRko	CTGTGTGTTCTGCAAGAAAT	TGG	-1.346	213	292	-	305	3	27623895
+FGR	CRISPRko	TGCCAAAGAGGGCAGCTTCT	GGG	-1.052	224	293	+	299	12	27613317
+FGR	CRISPRko	AGGCCATGCCCTCAGCTACC	TGG	-0.7641	246	294	+	277	11	27614582
+FGR	CRISPRko	GCCTTCGGGGACATGGTGCC	CGG	-0.3646	293	295	+	236	9	27615564
+FGR	CRISPRko	TCTCCCAGGCGTCCTTGGCC	AGG	-0.3493	298	296	+	233	8	27615772
+FGR	CRISPRko	GGGCTGCCATGTCCACCAAT	TGG	-0.7126	261	297	+	271	10	27614869
+FGR	CRISPRko	GGCCAAGGAGGATGCTGGCC	TGG	-0.9687	238	298	-	294	3	27623859
+FGR	CRISPRko	CCCCCAGGTAGCTGAGGGCA	TGG	-0.8259	254	299	-	281	11	27614574
+FGR	CRISPRko	GAAGCCGCAGACGCTGGGCC	TGG	-0.8693	251	300	-	286	8	27615779

resources/KO_SP_mouse.txt

@@ -0,0 +1,1001 @@
+Target Gene Symbol	CRISPR Mechanism	sgRNA Sequence	PAM Sequence	On-Target Efficacy Score	Off-Target Rank	Combined Rank	Strand of sgRNA	On-Target Rank	Exon Number	sgRNA Cut Position (1-based)
+Gnai3	CRISPRko	GATCGACCGCAACTTGCGGG	AGG	1.403	11	1	-	1	1	108145936
+Gnai3	CRISPRko	ACGTTTATGGCGAGATGGCG	GGG	0.6621	3	2	-	14	4	108118362
+Gnai3	CRISPRko	CCCCGTCCTCCCGCAAGTTG	CGG	0.5423	1	3	+	21	1	108145941
+Gnai3	CRISPRko	AGATGATCGACCGCAACTTG	CGG	0.5095	5	4	-	22	1	108145940
+Gnai3	CRISPRko	GGCTCGTATGATTGCAATGA	TGG	0.3755	2	5	+	29	3	108123601
+Gnai3	CRISPRko	AAAGATCCGAACGAAAAAAG	TGG	0.6456	15	6	-	17	6	108112566
+Gnai3	CRISPRko	GAACCGAAGAAAAGATACCA	AGG	0.8273	25	7	-	8	8	108109541
+Gnai3	CRISPRko	TCATGAGGACGGCTATTCAG	AGG	1.257	33	8	-	2	3	108123661
+Gnai3	CRISPRko	GGCATGCTTTAGCAGGTCCA	GGG	0.6607	22	9	-	15	4	108118335
+Gnai3	CRISPRko	AGGTTCCAATACATACGAAG	AGG	1.122	34	10	-	4	8	108109598
+Gnai3	CRISPRko	TTCGTATGTATTGGAACCTG	AGG	0.5686	23	11	+	20	8	108109613
+Gnai3	CRISPRko	ATCAATCTTCAACCGTCCCA	TGG	0.3528	16	12	+	31	3	108123580
+Gnai3	CRISPRko	ATTGCAATCATACGAGCCAT	GGG	0.08449	12	13	-	37	3	108123585
+Gnai3	CRISPRko	TTGCTGAGTTGGAATGTAGT	TGG	-0.09332	7	14	+	45	5	108115854
+Gnai3	CRISPRko	AAACGTTTATGGCGAGATGG	CGG	0.06188	14	15	-	39	4	108118364
+Gnai3	CRISPRko	ATCATTGAGCTGATATTCCC	TGG	0.08843	19	16	+	35	4	108118329
+Gnai3	CRISPRko	AGCTAAAACAAATAACTGTC	GGG	-0.1536	8	17	+	47	4	108118446
+Gnai3	CRISPRko	ATCTGTTATCCAGAATACAC	AGG	0.7066	45	18	-	13	7	108111940
+Gnai3	CRISPRko	AGTTGGAATGTAGTTGGTCT	GGG	-0.0636	21	19	+	44	5	108115860
+Gnai3	CRISPRko	GCTCAACGTGCAGCCCATGG	CGG	1.153	63	20	+	3	1	108146005
+Gnai3	CRISPRko	CAGTGATTACGACCTTGTTC	TGG	-0.293	13	21	-	56	6	108112496
+Gnai3	CRISPRko	CAGCTAAAACAAATAACTGT	CGG	-0.3368	9	22	+	60	4	108118445
+Gnai3	CRISPRko	AAAACAACTTAAAGGAATGT	GGG	0.04791	28	23	-	42	8	108109437
+Gnai3	CRISPRko	GGGCTGCACGTTGAGCGCCG	AGG	0.5956	52	24	-	18	1	108145987
+Gnai3	CRISPRko	GGAATGTGGGCTTTATTGAG	AGG	0.08778	37	25	-	36	8	108109424
+Gnai3	CRISPRko	ATTAAACGTTTATGGCGAGA	TGG	-0.5497	4	26	-	69	4	108118367
+Gnai3	CRISPRko	ATGAGAAATACTTACTGCTC	TGG	0.3944	47	27	+	27	3	108123544
+Gnai3	CRISPRko	CAATCATACGAGCCATGGGA	CGG	0.786	68	28	-	10	3	108123581
+Gnai3	CRISPRko	GACAGCAATTATCTTTTGTG	TGG	-0.9524	6	29	-	75	6	108112523
+Gnai3	CRISPRko	CGGACAAGAGTGAAGACTAC	AGG	-0.1545	35	30	-	48	5	108115811
+Gnai3	CRISPRko	GAGCGCCGAGGACAAGGCGG	CGG	1.012	78	31	-	6	1	108145975
+Gnai3	CRISPRko	AGCTGCCTCTTCGTATGTAT	TGG	-0.4959	20	32	+	65	8	108109604
+Gnai3	CRISPRko	TTAGCTGGGAGTGCTGAAGA	AGG	0.7943	76	33	-	9	4	108118418
+Gnai3	CRISPRko	GGCGCTCAACGTGCAGCCCA	TGG	0.7618	74	34	+	11	1	108146002
+Gnai3	CRISPRko	GATGATCGACCGCAACTTGC	GGG	-0.1053	41	35	-	46	1	108145939
+Gnai3	CRISPRko	AAAAACAACTTAAAGGAATG	TGG	-0.4039	29	36	-	63	8	108109438
+Gnai3	CRISPRko	CATTGCAATCATACGAGCCA	TGG	-0.3648	31	37	-	61	3	108123586
+Gnai3	CRISPRko	AGAGTTCCTTGAAGGTGAAG	TGG	0.6458	77	38	+	16	5	108115787
+Gnai3	CRISPRko	AGTGAAGACTACAGGCATTG	TGG	0.7098	85	39	-	12	5	108115803
+Gnai3	CRISPRko	GGACGGTTGAAGATTGATTT	TGG	-1.141	17	40	-	80	3	108123564
+Gnai3	CRISPRko	TTACGACCTTGTTCTGGCTG	AGG	0.05151	58	41	-	41	6	108112490
+Gnai3	CRISPRko	ACCGCAACTTGCGGGAGGAC	GGG	-0.329	40	42	-	59	1	108145931
+Gnai3	CRISPRko	TTCGGTTCAGATCTTCAAAC	TGG	-0.2941	43	43	+	57	8	108109567
+Gnai3	CRISPRko	TGAAGAATCATTCATGAGGA	CGG	0.05575	62	44	-	40	3	108123672
+Gnai3	CRISPRko	CTTGAAGTAGAGTTCCTTGA	AGG	-0.2583	49	45	+	55	5	108115779
+Gnai3	CRISPRko	ATGGCGAGATGGCGGGGTAC	AGG	-0.3696	42	46	-	62	4	108118356
+Gnai3	CRISPRko	GACCGCAACTTGCGGGAGGA	CGG	-0.2566	50	47	-	54	1	108145932
+Gnai3	CRISPRko	CATCATTAAAAACAACTTAA	AGG	-1.767	10	48	-	95	8	108109445
+Gnai3	CRISPRko	CCGAAGAAAAGATACCAAGG	AGG	0.9863	98	49	-	7	8	108109538
+Gnai3	CRISPRko	ATTTAGCTACCTAAATGATT	TGG	-1.357	18	50	-	87	5	108115881
+Gnai3	CRISPRko	GTTGAGCGCCGAGGACAAGG	CGG	1.094	100	51	-	5	1	108145978
+Gnai3	CRISPRko	AACGTTTATGGCGAGATGGC	GGG	-0.211	53	52	-	53	4	108118363
+Gnai3	CRISPRko	GAAGCTGCTGCTGCTCGGTG	AGG	0.4147	80	53	-	26	1	108145888
+Gnai3	CRISPRko	CCTCATCCTCAGCCAGAACA	AGG	0.58	88	54	+	19	6	108112495
+Gnai3	CRISPRko	GAGTTCCTTGAAGGTGAAGT	GGG	0.4217	83	55	+	24	5	108115788
+Gnai3	CRISPRko	ATTTCATGCTTTCATGCATT	CGG	-1.148	27	56	+	81	7	108112081
+Gnai3	CRISPRko	AGGACAAGGCGGCGGTGGAG	CGG	0.08088	70	57	-	38	1	108145967
+Gnai3	CRISPRko	GGGTACAGGCATGCTTTAGC	AGG	-0.4894	46	58	-	64	4	108118342
+Gnai3	CRISPRko	CCGCAACTTGCGGGAGGACG	GGG	-0.3042	54	59	-	58	1	108145930
+Gnai3	CRISPRko	CACGTTGAGCGCCGAGGACA	AGG	0.4909	89	60	-	23	1	108145981
+Gnai3	CRISPRko	GAAGTGAAGCTGCTGCTGCT	CGG	0.2223	81	61	-	32	1	108145893
+Gnai3	CRISPRko	GAAGTGAGTGTAGACCTCCT	TGG	0.3833	86	62	+	28	8	108109535
+Gnai3	CRISPRko	TCTGGATAACAGATTGTTAA	TGG	-0.7439	44	63	+	71	7	108111960
+Gnai3	CRISPRko	TCTGGCTGAGGATGAGGAAA	TGG	0.4167	90	64	-	25	6	108112478
+Gnai3	CRISPRko	CGCCGAGGACAAGGCGGCGG	TGG	0.2162	82	65	-	33	1	108145972
+Gnai3	CRISPRko	CAGCAGCAGCTTCACTTCTT	TGG	-1.446	26	66	+	90	1	108145909
+Gnai3	CRISPRko	AGGCATGCTTTAGCAGGTCC	AGG	-0.1558	67	67	-	50	4	108118336
+Gnai3	CRISPRko	TTACTTCATCTGTTTCACGA	TGG	-0.1548	69	68	+	49	2	108123808
+Gnai3	CRISPRko	CAACTCAGCAAGATGTTCTT	CGG	-1.149	38	69	-	82	5	108115831
+Gnai3	CRISPRko	GACGGTTGAAGATTGATTTT	GGG	-1.322	36	70	-	84	3	108123563
+Gnai3	CRISPRko	CCTCCTTGGTATCTTTTCTT	CGG	-1.544	30	71	+	92	8	108109549
+Gnai3	CRISPRko	CCTTGTTCTGGCTGAGGATG	AGG	-0.001719	79	72	-	43	6	108112484
+Gnai3	CRISPRko	GTGAATCCACTTTTTTCGTT	CGG	-2.143	24	73	+	99	6	108112571
+Gnai3	CRISPRko	ACGGTTGAAGATTGATTTTG	GGG	-0.6936	55	74	-	70	3	108123562
+Gnai3	CRISPRko	TTACTCAGAATGTTTGATGT	AGG	0.1387	92	75	-	34	6	108112594
+Gnai3	CRISPRko	ACTTTTTTCGTTCGGATCTT	TGG	-1.923	32	76	+	97	6	108112579
+Gnai3	CRISPRko	GCGGGAGGACGGGGAGAAAG	CGG	0.3603	101	77	-	30	1	108145921
+Gnai3	CRISPRko	GACAGTTATTTGTTTTAGCT	GGG	-1.552	39	78	-	93	4	108118432
+Gnai3	CRISPRko	TAATAAGAAAGACCTTTTTG	AGG	-1.272	51	79	-	83	7	108111989
+Gnai3	CRISPRko	ACAGCATTTGTAACAACAAA	TGG	-1.048	59	80	-	77	7	108112041
+Gnai3	CRISPRko	GTCATGACTTCAGAACTAGC	AGG	-0.1851	87	81	-	52	4	108118394
+Gnai3	CRISPRko	TTTTTGAAGAATCATTCATG	AGG	-1.533	48	82	-	91	3	108123676
+Gnai3	CRISPRko	AAGTGGATTCACTGTTTTGA	GGG	-0.9819	65	83	-	76	6	108112549
+Gnai3	CRISPRko	CTGCACATTTTTGGTGTCTG	TGG	-1.341	56	84	+	86	8	108109505
+Gnai3	CRISPRko	TTTTTAGGCGCTGGAGAATC	TGG	-1.322	57	85	-	85	2	108123828
+Gnai3	CRISPRko	AAAGTGGATTCACTGTTTTG	AGG	-1.115	64	86	-	79	6	108112550
+Gnai3	CRISPRko	CGAAGAACATCTTGCTGAGT	TGG	-0.7631	73	87	+	73	5	108115843
+Gnai3	CRISPRko	GGAGACCCACTTCACCTTCA	AGG	-0.7621	75	88	-	72	5	108115782
+Gnai3	CRISPRko	CTCAGAATGTTTGATGTAGG	TGG	-0.1563	99	89	-	51	6	108112591
+Gnai3	CRISPRko	TTTGTTTTTTTTTTAGGCGC	TGG	-1.428	61	90	-	89	2	108123837
+Gnai3	CRISPRko	GAGTTGGAATGTAGTTGGTC	TGG	-0.5296	84	91	+	67	5	108115859
+Gnai3	CRISPRko	AAAAACAAACTGCACATTTT	TGG	-2.033	60	92	+	98	8	108109496
+Gnai3	CRISPRko	CTCCACCGCCGCCTTGTCCT	CGG	-0.5009	94	93	+	66	1	108145981
+Gnai3	CRISPRko	ATTTTTGGTGTCTGTGGCAC	AGG	-0.5418	95	94	+	68	8	108109511
+Gnai3	CRISPRko	ATATTCTATCCAAATCATTT	AGG	-0.8896	91	95	+	74	5	108115883
+Gnai3	CRISPRko	GTTTGTTTTTGATGCTGTTA	CGG	-1.628	72	96	-	94	8	108109472
+Gnai3	CRISPRko	CGACAGTTATTTGTTTTAGC	TGG	-2.146	66	97	-	100	4	108118433
+Gnai3	CRISPRko	CGCCCTTTACCTGTGTATTC	TGG	-1.898	71	98	+	96	7	108111942
+Gnai3	CRISPRko	TTTTTGAGGAAAAAATAAAG	AGG	-1.054	96	99	-	78	7	108111975
+Gnai3	CRISPRko	CAGGCGTGATTAAACGTTTA	TGG	-1.409	97	100	-	88	4	108118375
+Gnai3	CRISPRko	TCTTTATTTTTTCCTCAAAA	AGG	-2.383	93	101	+	101	7	108111988
+Pbsn	CRISPRko	ACAGTCGCTCACACATGATG	AGG	0.9547	2	1	-	4	1	77853481
+Pbsn	CRISPRko	CAGTCGCTCACACATGATGA	GGG	0.996	13	2	-	2	1	77853480
+Pbsn	CRISPRko	AGGCAAACAACTGAATAAGG	AGG	0.5966	9	3	-	8	5	77842596
+Pbsn	CRISPRko	AAGAAATGATGTCACACGAG	TGG	0.8162	14	4	-	6	4	77842915
+Pbsn	CRISPRko	TGACGGAAGTAGGTTCTCAA	TGG	0.5826	12	5	+	9	2	77848043
+Pbsn	CRISPRko	GTACAGCGTATCATGGACAC	GGG	0.9115	19	6	-	5	5	77842520
+Pbsn	CRISPRko	TGTACAGCGTATCATGGACA	CGG	0.0002799	3	7	-	22	5	77842521
+Pbsn	CRISPRko	AGAGAATGTACAGCGTATCA	TGG	0.1621	10	8	-	16	5	77842527
+Pbsn	CRISPRko	ACACCGTGAAACGCAGCAAA	AGG	-0.1838	4	9	-	25	5	77842616
+Pbsn	CRISPRko	AATGATGTCACACGAGTGGC	TGG	0.09016	11	10	-	20	4	77842911
+Pbsn	CRISPRko	GCTAAGTAAATTGTTTGCCA	AGG	-0.02531	8	11	+	23	2	77848100
+Pbsn	CRISPRko	TCCTCCCACACAAAATGTGA	CGG	0.5618	22	12	+	10	2	77848026
+Pbsn	CRISPRko	TAAAAAAATAGAGGTAGACT	TGG	0.09916	16	13	+	18	2	77847996
+Pbsn	CRISPRko	TTACTTAGCTGCCAGTACCA	TGG	0.9874	33	14	-	3	2	77848075
+Pbsn	CRISPRko	TTTGCCAAGGCCCGTCAATC	TGG	-0.8308	1	15	+	37	2	77848113
+Pbsn	CRISPRko	ACTTCCGTCACATTTTGTGT	GGG	-0.6753	6	16	-	34	2	77848019
+Pbsn	CRISPRko	AACTGTATAAAGTCATAGGA	AGG	0.1403	25	17	-	17	3	77845053
+Pbsn	CRISPRko	CTAGCACATGCAGTGTGAGC	AGG	0.2015	27	18	+	15	1	77853458
+Pbsn	CRISPRko	AAAAGGCAAACAACTGAATA	AGG	-0.8763	5	19	-	38	5	77842599
+Pbsn	CRISPRko	TCTTACCTATCTCAGATGAA	GGG	0.3879	30	20	-	13	4	77843006
+Pbsn	CRISPRko	CTTACTTAATAAAAAAATAG	AGG	-0.2322	17	21	+	26	2	77847987
+Pbsn	CRISPRko	TGCCAACTGTATAAAGTCAT	AGG	-0.3787	15	22	-	29	3	77845057
+Pbsn	CRISPRko	ACCATGGAAAAGATAAATGA	AGG	1.065	45	23	-	1	2	77848059
+Pbsn	CRISPRko	ATTTATCTTTTCCATGGTAC	TGG	-1.156	7	24	+	40	2	77848075
+Pbsn	CRISPRko	ACACAAAATGTGACGGAAGT	AGG	-0.2527	21	25	+	27	2	77848033
+Pbsn	CRISPRko	ACTGAATAAGGAGGAGATGA	CGG	0.7589	42	26	-	7	5	77842587
+Pbsn	CRISPRko	TGTGATGAGTCTCAAAAAGA	AGG	0.3788	36	27	-	14	1	77853414
+Pbsn	CRISPRko	GACGGAGTTCATGAACTTAG	TGG	0.04124	31	28	-	21	5	77842569
+Pbsn	CRISPRko	GCACATGCAGTGTGAGCAGG	AGG	0.5545	41	29	+	11	1	77853461
+Pbsn	CRISPRko	TTCCTATGACTTTATACAGT	TGG	-0.5741	23	30	+	32	3	77845066
+Pbsn	CRISPRko	CTTTTTGAGACTCATCACAC	TGG	-0.772	20	31	+	36	1	77853429
+Pbsn	CRISPRko	TCCGTCACATTTTGTGTGGG	AGG	-0.664	24	32	-	33	2	77848016
+Pbsn	CRISPRko	CTGCTCACACTGCATGTGCT	AGG	0.09782	38	33	-	19	1	77853446
+Pbsn	CRISPRko	GTCTTACCTATCTCAGATGA	AGG	-0.04505	34	34	-	24	4	77843007
+Pbsn	CRISPRko	AGTGGAAGAAATGGGCATTG	AGG	0.5204	48	35	-	12	5	77842551
+Pbsn	CRISPRko	TTTTCCAGATTGACGGGCCT	TGG	-2.062	18	36	-	47	2	77848106
+Pbsn	CRISPRko	TACTTCCGTCACATTTTGTG	TGG	-1.062	28	37	-	39	2	77848020
+Pbsn	CRISPRko	CATGAACTTAGTGGAAGAAA	TGG	-0.3834	39	38	-	30	5	77842560
+Pbsn	CRISPRko	ACACGAGTGGCTGGAGTTTT	GGG	-1.667	26	39	-	44	4	77842902
+Pbsn	CRISPRko	CACACGAGTGGCTGGAGTTT	TGG	-1.227	29	40	-	41	4	77842903
+Pbsn	CRISPRko	CATAGCATTCATGTTAAAGA	TGG	-0.3215	46	41	-	28	4	77842981
+Pbsn	CRISPRko	GCCTTCATTTATCTTTTCCA	TGG	-0.6999	40	42	+	35	2	77848069
+Pbsn	CRISPRko	ATGAACTTAGTGGAAGAAAT	GGG	-0.4228	47	43	-	31	5	77842559
+Pbsn	CRISPRko	CTGCTTTTTTTCCAGATTGA	CGG	-2.181	32	44	-	49	2	77848113
+Pbsn	CRISPRko	CGACATTTGTTTTTCAGGAA	AGG	-1.82	37	45	-	45	3	77845087
+Pbsn	CRISPRko	TGCTTTTTTTCCAGATTGAC	GGG	-2.104	35	46	-	48	2	77848112
+Pbsn	CRISPRko	CTGATCTTGTTGAGTCACTA	AGG	-1.608	44	47	+	43	6	77841883
+Pbsn	CRISPRko	CACTAAGGTTTGATCTTGCT	TGG	-2.051	43	48	+	46	6	77841898
+Pbsn	CRISPRko	GACATTTGTTTTTCAGGAAA	GGG	-1.602	49	49	-	42	3	77845086
+Cdc45	CRISPRko	TGACCACGTCCAGTATACGC	TGG	1.661	4	1	-	1	4	18810451
+Cdc45	CRISPRko	CCGTCATAACCACCGAAACG	AGG	1.129	3	2	-	12	11	18795883
+Cdc45	CRISPRko	GCTGATACAGTACCAGGCAG	AGG	1.257	21	3	+	7	12	18795221
+Cdc45	CRISPRko	TCTTGGCGAGCTCTAGACCA	AGG	1.593	38	4	+	2	15	18787042
+Cdc45	CRISPRko	ATATCTTGAACATAAAGAGC	AGG	0.8524	18	5	-	29	4	18810394
+Cdc45	CRISPRko	CACAGACCAGAGCTTAAACC	TGG	0.7997	14	6	+	34	12	18795153
+Cdc45	CRISPRko	GCCCCGCTGAGCGTGGAACA	GGG	0.7389	9	7	-	44	17	18785133
+Cdc45	CRISPRko	GCCCTGGGAAGTGACGAGGT	TGG	1.096	40	8	+	15	15	18786974
+Cdc45	CRISPRko	GAACCAGCGTATACTGGACG	TGG	0.7791	24	9	+	37	4	18810459
+Cdc45	CRISPRko	GACGTGGTCACACTGAAACA	GGG	1.142	51	10	+	10	4	18810475
+Cdc45	CRISPRko	TCATAACCACCGAAACGAGG	CGG	0.7558	25	11	-	40	11	18795880
+Cdc45	CRISPRko	TGATCTTGTCATGCACCCAC	TGG	0.8642	42	12	+	27	10	18797370
+Cdc45	CRISPRko	GAGGATCTTGCAAGCACACA	AGG	0.5975	15	13	+	55	3	18811325
+Cdc45	CRISPRko	CTACGAGACGGTCCACAACC	AGG	0.5233	5	14	-	67	2	18811520
+Cdc45	CRISPRko	GCAAAGATACTCATACTCGA	AGG	0.5211	7	15	+	69	11	18795832
+Cdc45	CRISPRko	TAACCACCGAAACGAGGCGG	AGG	0.5557	26	16	-	61	11	18795877
+Cdc45	CRISPRko	GAAGCGCACACGGTTAGAAG	AGG	0.5735	30	17	-	59	6	18807285
+Cdc45	CRISPRko	GCCTGGTACTGTATCAGCAC	TGG	0.6665	47	18	-	46	12	18795205
+Cdc45	CRISPRko	GATATCCAACAGGTCCACGT	TGG	1.12	81	19	+	13	5	18808833
+Cdc45	CRISPRko	CTGGTCTGTCAGTCCAACAA	TGG	0.5951	43	20	+	56	10	18797389
+Cdc45	CRISPRko	TCCGAGCTATCAGTCTCTGG	GGG	0.6955	56	21	+	45	17	18785099
+Cdc45	CRISPRko	GATGGCTGCCCCGCTGAGCG	TGG	0.765	62	22	-	39	17	18785140
+Cdc45	CRISPRko	GTATGAATATTATGGGACAT	CGG	0.613	49	23	-	52	8	18801806
+Cdc45	CRISPRko	CATAAACTGTGGAGCCAACG	TGG	0.5404	36	24	-	65	5	18808836
+Cdc45	CRISPRko	TTGTTGGACTGACAGACCAG	TGG	0.7848	67	25	-	35	10	18797375
+Cdc45	CRISPRko	AACCTGGACAAGCTATACCT	TGG	0.7465	61	26	-	42	15	18787048
+Cdc45	CRISPRko	AGTGCTCACCGAGTACACAA	AGG	1.021	88	27	+	17	16	18786777
+Cdc45	CRISPRko	GACGGGACACATGACGCTGC	AGG	0.4912	33	28	+	72	11	18795912
+Cdc45	CRISPRko	ATTGTGTAGAGTCCGAGAGC	TGG	0.8961	84	29	+	23	18	18784839
+Cdc45	CRISPRko	GGTGGCAAAGACGACATCAC	TGG	0.651	59	30	+	48	14	18793272
+Cdc45	CRISPRko	GTTCGTGACCGATTTCCGCA	AGG	0.1934	1	31	-	106	2	18811547
+Cdc45	CRISPRko	GCAGTGACACAAGAGCGTCC	AGG	0.4615	37	32	+	74	19	18781923
+Cdc45	CRISPRko	CGCCAGGTCAAACATCACCA	TGG	0.9847	92	33	+	19	9	18798728
+Cdc45	CRISPRko	CGTCCGAGGCCACAAAGAGA	AGG	0.3022	17	34	+	100	3	18811354
+Cdc45	CRISPRko	TCTCTACTGCTCACTCATGG	AGG	0.868	95	35	-	25	15	18786935
+Cdc45	CRISPRko	CAGCAGCCAGGTTTAAGCTC	TGG	0.1455	12	36	-	110	12	18795148
+Cdc45	CRISPRko	ATCTCTAAAGATGTCGTCGT	AGG	0.03053	2	37	+	122	6	18807375
+Cdc45	CRISPRko	ACACAAGGCATCCACGTCCG	AGG	0.9375	105	38	+	22	3	18811340
+Cdc45	CRISPRko	TGTTGGACTGACAGACCAGT	GGG	0.4463	52	39	-	78	10	18797374
+Cdc45	CRISPRko	AGGAGTTCCTTGCAGACATG	GGG	0.8317	101	40	-	31	12	18795100
+Cdc45	CRISPRko	GGAACAGGGCACAGTGACCG	TGG	1.008	115	41	-	18	17	18785119
+Cdc45	CRISPRko	GCCCTGTTCCACGCTCAGCG	GGG	1.379	128	42	+	5	17	18785143
+Cdc45	CRISPRko	TTAAGCTCTGGTCTGTGCAT	GGG	0.5766	77	43	-	58	12	18795136
+Cdc45	CRISPRko	ATGAAGTACGTGACTGATGT	TGG	0.01501	11	44	-	125	11	18795927
+Cdc45	CRISPRko	CAAGCAGAAATTTCAGTCCA	TGG	0.1144	22	45	-	117	13	18794826
+Cdc45	CRISPRko	AGTTCTTGCCACCCAGAAAC	CGG	0.6207	94	46	+	50	4	18810437
+Cdc45	CRISPRko	TGCTCTCTGTAGACTGCACC	CGG	-0.08926	13	47	-	132	11	18795848
+Cdc45	CRISPRko	GGCCATGGTGATGTTTGACC	TGG	0.5219	85	48	-	68	9	18798719
+Cdc45	CRISPRko	GACCAAGGTATAGCTTGTCC	AGG	0.5018	86	49	+	71	15	18787057
+Cdc45	CRISPRko	AAACCGGAACCAGCGTATAC	TGG	-0.1135	23	50	+	134	4	18810453
+Cdc45	CRISPRko	GCCCTGTTTCAGGAGTAACC	TGG	0.366	69	51	-	90	15	18787064
+Cdc45	CRISPRko	CCGAGCTATCAGTCTCTGGG	GGG	1.049	144	52	+	16	17	18785100
+Cdc45	CRISPRko	GCATGGGCAGAAACGACTCC	AGG	0.4252	83	53	-	80	12	18795120
+Cdc45	CRISPRko	CCGTCTCGTAGAACTCCTTG	CGG	0.4899	90	54	+	73	2	18811543
+Cdc45	CRISPRko	CGTCGTAGGCAGGAACCTCA	AGG	0.1332	50	55	+	114	6	18807389
+Cdc45	CRISPRko	CATACTGCTCGTAGTCAAAG	AGG	0.2035	60	56	+	105	8	18801835
+Cdc45	CRISPRko	CTCGTTTCGGTGGTTATGAC	GGG	-0.3897	6	57	+	160	11	18795895
+Cdc45	CRISPRko	GGTACAGAGACAGCTGGCGA	TGG	1.546	164	58	+	3	15	18786995
+Cdc45	CRISPRko	GAGGTTGGTACAGAGACAGC	TGG	1.528	166	59	+	4	15	18786989
+Cdc45	CRISPRko	GTGTGCTTGCAAGATCCTCC	AGG	0.5545	108	60	-	62	3	18811310
+Cdc45	CRISPRko	CCTGGCGTGGATGATGTCCA	AGG	0.7521	136	61	-	41	9	18798701
+Cdc45	CRISPRko	TGGTCTGTTGTGTGGCTTGG	AGG	0.4565	104	62	+	75	15	18787015
+Cdc45	CRISPRko	TCTCTTTGTGGCCTCGGACG	TGG	0.9753	158	63	-	21	3	18811340
+Cdc45	CRISPRko	GGATGCTGGCTTGGAAAACA	GGG	0.5819	123	64	+	57	16	18786831
+Cdc45	CRISPRko	AAGGGCCCTGGGAAGTGACG	AGG	1.308	174	65	+	6	15	18786970
+Cdc45	CRISPRko	AGGCTCTCGATAGCCTCTCC	AGG	0.1148	65	66	-	116	14	18793183
+Cdc45	CRISPRko	CCCCTTCAAGGAAACGTCCA	TGG	0.01742	58	67	+	123	13	18794820
+Cdc45	CRISPRko	AGCGGGGCAGCCATCACCAG	GGG	1.141	171	68	+	11	17	18785159
+Cdc45	CRISPRko	CAGGAGTTCCTTGCAGACAT	GGG	0.1397	71	69	-	111	12	18795101
+Cdc45	CRISPRko	GCAGTAGAGAAAAGGGCCCT	GGG	0.8642	157	70	+	26	15	18786959
+Cdc45	CRISPRko	GGTATTCAAAGCTCACCTGG	AGG	0.8197	152	71	+	32	3	18811306
+Cdc45	CRISPRko	ACGATGCAAGCTGCTGCCCC	TGG	0.8472	155	72	-	30	17	18785164
+Cdc45	CRISPRko	CGCTGGTTCCGGTTTCTGGG	TGG	0.5234	119	73	-	66	4	18810434
+Cdc45	CRISPRko	CAAATTGCTTATTAAACAAG	AGG	-0.5435	19	74	-	169	6	18807405
+Cdc45	CRISPRko	CCGCAAGGAGTTCTACGAGA	CGG	0.3034	89	75	-	99	2	18811532
+Cdc45	CRISPRko	ACCAGTGCTGATACAGTACC	AGG	0.4496	112	76	+	77	12	18795215
+Cdc45	CRISPRko	TGTTCTCCTCCGCCTCGTTT	CGG	-0.4351	29	77	+	161	11	18795882
+Cdc45	CRISPRko	ACCAACCTCGTCACTTCCCA	GGG	0.3445	98	78	-	94	15	18786964
+Cdc45	CRISPRko	TGGTGATGTTTGACCTGGCG	TGG	-0.3136	35	79	-	157	9	18798714
+Cdc45	CRISPRko	GAATGTGTACAATGACACTC	AGG	0.5987	138	80	-	54	5	18808737
+Cdc45	CRISPRko	CCATGGACGTTTCCTTGAAG	GGG	0.45	117	81	-	76	13	18794809
+Cdc45	CRISPRko	TCACAACGTTCACTGGCCTG	TGG	0.4184	111	82	+	82	5	18808769
+Cdc45	CRISPRko	TATCCGAGCTATCAGTCTCT	GGG	-0.2411	45	83	+	149	17	18785097
+Cdc45	CRISPRko	GAAGATCTTTCAGACAGTGA	CGG	0.04223	73	84	-	121	6	18807329
+Cdc45	CRISPRko	TCTCCTCCGCCTCGTTTCGG	TGG	0.5196	126	85	+	70	11	18795885
+Cdc45	CRISPRko	AGGACCTGAATGACATGCTG	TGG	1.12	183	86	-	14	9	18798681
+Cdc45	CRISPRko	GGACGTGGTCACACTGAAAC	AGG	-0.2441	46	87	+	151	4	18810474
+Cdc45	CRISPRko	CAGCGGGGCAGCCATCACCA	GGG	0.9767	178	88	+	20	17	18785158
+Cdc45	CRISPRko	CCTTGGACATCATCCACGCC	AGG	-0.1955	57	89	+	141	9	18798712
+Cdc45	CRISPRko	GAGGAGGCAGCGCAGGGAGT	GGG	0.6075	145	90	-	53	7	18805398
+Cdc45	CRISPRko	TTGGAAAACAGGGTGACATC	TGG	-0.3044	44	91	+	155	16	18786841
+Cdc45	CRISPRko	GGCAGAGACACTCACCTGAG	AGG	1.249	192	92	+	8	18	18784808
+Cdc45	CRISPRko	ATCATACCCATTACCTGGAG	AGG	0.1472	93	93	+	109	14	18793181
+Cdc45	CRISPRko	TACTTCAGTAATTGAGCTGA	AGG	-0.07662	74	94	-	130	19	18781954
+Cdc45	CRISPRko	TTACTTACCCCATGTCTGCA	AGG	0.6202	153	95	+	51	12	18795104
+Cdc45	CRISPRko	ATACGCTGGTTCCGGTTTCT	GGG	-0.6801	31	96	-	173	4	18810437
+Cdc45	CRISPRko	CTGAGCAGACTCAAGGATGC	TGG	0.4011	122	97	+	83	16	18786817
+Cdc45	CRISPRko	TGGGTTCAAGCATAAATTCC	TGG	-0.5175	39	98	-	166	14	18793284
+Cdc45	CRISPRko	GCTGGCGATGGTCTGTTGTG	TGG	1.158	197	99	+	9	15	18787007
+Cdc45	CRISPRko	CTAAAGATGTCGTCGTAGGC	AGG	-0.7684	27	100	+	180	6	18807379
+Cdc45	CRISPRko	GTTGTACAGGCTTTCATGGA	GGG	0.3676	120	101	+	88	12	18795192
+Cdc45	CRISPRko	ACTCATACTCGAAGGAGATC	CGG	0.2669	109	102	+	101	11	18795840
+Cdc45	CRISPRko	CATACCACAGCATGTCATTC	AGG	-1.093	8	103	+	203	9	18798688
+Cdc45	CRISPRko	CACAACGTTCACTGGCCTGT	GGG	-0.2174	64	104	+	147	5	18808770
+Cdc45	CRISPRko	TACACATTCACAACGTTCAC	TGG	-0.7437	34	105	+	178	5	18808762
+Cdc45	CRISPRko	AAGAATCTGCAAATAAATTT	GGG	-0.9505	20	106	-	194	13	18794767
+Cdc45	CRISPRko	CAGCAGAAAGCACCAGCTCT	CGG	-0.1494	78	107	-	137	18	18784840
+Cdc45	CRISPRko	CCTCGTTTCGGTGGTTATGA	CGG	-0.7996	32	108	+	183	11	18795894
+Cdc45	CRISPRko	AGGAGATAGTGGAGCGAAAC	AGG	-0.8733	28	109	-	189	7	18805423
+Cdc45	CRISPRko	CATCGTCGATTCTTTGTCTA	TGG	-1.221	10	110	+	208	17	18785192
+Cdc45	CRISPRko	AGGATGCTGGCTTGGAAAAC	AGG	-0.244	68	111	+	150	16	18786830
+Cdc45	CRISPRko	ATGGAGAGTCCTGAGAAGGA	TGG	0.7829	182	112	-	36	14	18793229
+Cdc45	CRISPRko	ACTCTCCATTAGAGACATGG	TGG	0.8542	190	113	+	28	14	18793254
+Cdc45	CRISPRko	TTTTGGTCGGGCTTTTGAGA	AGG	-0.5097	54	114	-	165	18	18784863
+Cdc45	CRISPRko	CAGGCTGAAGGATATCCAAC	AGG	0.3563	131	115	+	92	5	18808823
+Cdc45	CRISPRko	GCAGGGAGTGGGAGGCCCGA	AGG	0.7999	191	116	-	33	7	18805387
+Cdc45	CRISPRko	ACAGGGCACAGTGACCGTGG	TGG	0.7764	187	117	-	38	17	18785116
+Cdc45	CRISPRko	AACCGTGTGCGCTTCTCTGA	AGG	-0.389	66	118	+	159	6	18807304
+Cdc45	CRISPRko	ACAGTTTATGAGGATGAAAT	AGG	-0.2963	72	119	+	154	5	18808860
+Cdc45	CRISPRko	CAGACTCAAGGATGCTGGCT	TGG	-0.2738	76	120	+	152	16	18786822
+Cdc45	CRISPRko	GAAGAATCTGCAAATAAATT	TGG	-1.355	16	121	-	212	13	18794768
+Cdc45	CRISPRko	TACGAGCAGTATGAATATTA	TGG	-0.8425	41	122	-	187	8	18801814
+Cdc45	CRISPRko	GGGTGACATCTGGAGTGCCC	TGG	0.3664	140	123	+	89	16	18786851
+Cdc45	CRISPRko	TATTTCATCCTCATAAACTG	TGG	0.8821	205	124	-	24	5	18808847
+Cdc45	CRISPRko	GTGCCCTGTTCCACGCTCAG	CGG	0.2064	130	125	+	104	17	18785141
+Cdc45	CRISPRko	TACAGTACCAGGCAGAGGCT	AGG	0.5579	175	126	+	60	12	18795226
+Cdc45	CRISPRko	CGTCCAGTATACGCTGGTTC	CGG	-0.3072	79	127	-	156	4	18810445
+Cdc45	CRISPRko	TGGAGCCAACGTGGACCTGT	TGG	0.2208	135	128	-	102	5	18808827
+Cdc45	CRISPRko	AGACTTTCAGCATTCAGTTT	GGG	-0.829	53	129	-	186	14	18793303
+Cdc45	CRISPRko	TCAGCGGGGCAGCCATCACC	AGG	0.5438	177	130	+	63	17	18785157
+Cdc45	CRISPRko	AGGACTCTCCATTAGAGACA	TGG	0.7449	198	131	+	43	14	18793251
+Cdc45	CRISPRko	TGCCCCGCTGAGCGTGGAAC	AGG	-0.4625	80	132	-	162	17	18785134
+Cdc45	CRISPRko	CAGTCCAACAATGGCCCACC	TGG	-0.1213	107	133	+	135	10	18797398
+Cdc45	CRISPRko	GAATGGGAGTCACCTGGTTG	TGG	0.2172	139	134	+	103	2	18811519
+Cdc45	CRISPRko	CTATCCGAGCTATCAGTCTC	TGG	-0.7697	63	135	+	181	17	18785096
+Cdc45	CRISPRko	CAGACTTTCAGCATTCAGTT	TGG	-0.9874	48	136	-	196	14	18793304
+Cdc45	CRISPRko	AGGGACAGACCACTTCATCC	AGG	0.379	159	137	-	86	14	18793203
+Cdc45	CRISPRko	CGATGGTCTGTTGTGTGGCT	TGG	-0.2142	103	138	+	146	15	18787012
+Cdc45	CRISPRko	TACCAACCTCGTCACTTCCC	AGG	0.06026	132	139	-	120	15	18786965
+Cdc45	CRISPRko	TGGAGCGAAACAGGAAGAGG	AGG	0.6424	203	140	-	49	7	18805414
+Cdc45	CRISPRko	TAGTGGAGCGAAACAGGAAG	AGG	-0.008863	125	141	-	127	7	18805417
+Cdc45	CRISPRko	CACAATTACTTTGACCTCTC	AGG	-1.023	55	142	-	199	18	18784811
+Cdc45	CRISPRko	TGTTGTACAGGCTTTCATGG	AGG	0.6577	207	143	+	47	12	18795191
+Cdc45	CRISPRko	CGTTGGCTCCACAGTTTATG	AGG	-0.2108	113	144	+	145	5	18808850
+Cdc45	CRISPRko	ATGTCTGCTGAGCAGACTCA	AGG	0.364	168	145	+	91	16	18786810
+Cdc45	CRISPRko	TGGTGTTGTACAGGCTTTCA	TGG	-0.4853	96	146	+	164	12	18795188
+Cdc45	CRISPRko	CCTTGGACCTAGCCTCTGCC	TGG	0.3192	163	147	-	97	12	18795222
+Cdc45	CRISPRko	GTCACTATTTCAGGAGATAG	TGG	-0.1574	121	148	-	139	7	18805434
+Cdc45	CRISPRko	GCTCAAGTCCTTTGTGTACT	CGG	-0.8686	75	149	-	188	16	18786774
+Cdc45	CRISPRko	TATCGAGAGCCTGGATGAAG	TGG	0.3677	176	150	+	87	14	18793205
+Cdc45	CRISPRko	CCTTCCAGGTGGGCCATTGT	TGG	-0.7284	87	151	-	177	10	18797391
+Cdc45	CRISPRko	CAAAGACGACATCACTGGCC	AGG	0.09367	150	152	+	119	14	18793277
+Cdc45	CRISPRko	GTTTTCCTTGCAGTCGGCCA	TGG	-0.8833	82	153	-	190	9	18798734
+Cdc45	CRISPRko	GAGCGTCCAGGAACTTGCTC	CGG	0.1117	156	154	+	118	19	18781935
+Cdc45	CRISPRko	ATCCGAGCTATCAGTCTCTG	GGG	-0.1744	134	155	+	140	17	18785098
+Cdc45	CRISPRko	CTCATACTCGAAGGAGATCC	GGG	0.1307	160	156	+	115	11	18795841
+Cdc45	CRISPRko	CATCTTTAGAGATGAAGCAG	AGG	-0.06861	146	157	-	129	6	18807354
+Cdc45	CRISPRko	GTCCCTGAGCCATCCTTCTC	AGG	-1.173	70	158	+	206	14	18793231
+Cdc45	CRISPRko	CAGGAAGAGGAGGCAGCGCA	GGG	0.3948	193	159	-	84	7	18805404
+Cdc45	CRISPRko	GTAGAACTCCTTGCGGAAAT	CGG	-0.6226	106	160	+	171	2	18811550
+Cdc45	CRISPRko	TTTTTGTGTGTGATACCCAC	AGG	-0.7607	99	161	-	179	5	18808774
+Cdc45	CRISPRko	GTCCTGAGAAGGATGGCTCA	GGG	0.5423	215	162	-	64	14	18793222
+Cdc45	CRISPRko	CAGGGCACAGTGACCGTGGT	GGG	-0.1098	147	163	-	133	17	18785115
+Cdc45	CRISPRko	CAAGCTGCTGCCCCTGGTGA	TGG	0.3947	196	164	-	85	17	18785158
+Cdc45	CRISPRko	GAGGCAGCGCAGGGAGTGGG	AGG	0.3333	189	165	-	95	7	18805395
+Cdc45	CRISPRko	TTCTGCTTGACTTGCTTCAG	GGG	-0.2081	142	166	+	144	13	18794851
+Cdc45	CRISPRko	CCCCCCAGAGACTGATAGCT	CGG	-0.9059	97	167	-	193	17	18785089
+Cdc45	CRISPRko	TGAGTGAGCAGTAGAGAAAA	GGG	0.4218	210	168	+	81	15	18786952
+Cdc45	CRISPRko	CTGTGTAGCTGGTGTTGTAC	AGG	-0.2044	148	169	+	143	12	18795179
+Cdc45	CRISPRko	TCTAATGGAGAGTCCTGAGA	AGG	0.3515	200	170	-	93	14	18793233
+Cdc45	CRISPRko	ACAGGAAGAGGAGGCAGCGC	AGG	0.4324	216	171	-	79	7	18805405
+Cdc45	CRISPRko	TATACGCTGGTTCCGGTTTC	TGG	-1.106	91	172	-	204	4	18810438
+Cdc45	CRISPRko	CACTTCTCCAGACTCACCTT	CGG	-0.2341	149	173	+	148	7	18805382
+Cdc45	CRISPRko	TTTTCTCTACTGCTCACTCA	TGG	-0.7742	118	174	-	182	15	18786938
+Cdc45	CRISPRko	TTCCTCCCTGGACAGGATGA	AGG	0.3038	202	175	-	98	14	18793338
+Cdc45	CRISPRko	TTGAGCTGAAGGCTGAGGAC	CGG	0.1375	188	176	-	113	19	18781943
+Cdc45	CRISPRko	CCAGGAGTTCCTTGCAGACA	TGG	-0.04304	173	177	-	128	12	18795102
+Cdc45	CRISPRko	AGCAGTAGAGAAAAGGGCCC	TGG	0.01721	181	178	+	124	15	18786958
+Cdc45	CRISPRko	TGGAGAGGCTATCGAGAGCC	TGG	-0.9547	110	179	+	195	14	18793196
+Cdc45	CRISPRko	TAAACAAGAGGATGACCTTG	AGG	0.1915	199	180	-	107	6	18807393
+Cdc45	CRISPRko	CCATGTCTGCAAGGAACTCC	TGG	-0.1274	172	181	+	136	12	18795113
+Cdc45	CRISPRko	TTTAAGCTCTGGTCTGTGCA	TGG	-0.1503	170	182	-	138	12	18795137
+Cdc45	CRISPRko	AGCCTTCAGAGAAGCGCACA	CGG	0.3303	214	183	-	96	6	18807295
+Cdc45	CRISPRko	CTTTGCCACCATGTCTCTAA	TGG	-1.322	100	184	-	211	14	18793248
+Cdc45	CRISPRko	ATCAGTCACGTACTTCATTC	TGG	-0.8096	127	185	+	184	11	18795943
+Cdc45	CRISPRko	GGTCACACTGAAACAGGGCC	TGG	-0.5749	141	186	+	170	4	18810480
+Cdc45	CRISPRko	AGTAATTGAGCTGAAGGCTG	AGG	0.1389	201	187	-	112	19	18781948
+Cdc45	CRISPRko	ATGAGTGAGCAGTAGAGAAA	AGG	0.1517	208	188	+	108	15	18786951
+Cdc45	CRISPRko	TCCATGGACGTTTCCTTGAA	GGG	-1.056	116	189	-	201	13	18794810
+Cdc45	CRISPRko	TTCTGTGCCTTTAGCTTTTT	TGG	-2.998	102	190	-	216	18	18784880
+Cdc45	CRISPRko	AGAGGAGGCAGCGCAGGGAG	TGG	-0.0004799	194	191	-	126	7	18805399
+Cdc45	CRISPRko	TGTGGCTTGGAGGTGCTTCT	TGG	-0.5309	154	192	+	167	15	18787025
+Cdc45	CRISPRko	GTCCATGGACGTTTCCTTGA	AGG	-0.8954	129	193	-	192	13	18794811
+Cdc45	CRISPRko	ATTTCTGCTTGACTTGCTTC	AGG	-1.222	114	194	+	209	13	18794849
+Cdc45	CRISPRko	TGAGGACCGGAGCAAGTTCC	TGG	-0.4805	162	195	-	163	19	18781930
+Cdc45	CRISPRko	AGTCCTGAGAAGGATGGCTC	AGG	-0.1959	184	196	-	142	14	18793223
+Cdc45	CRISPRko	CTTTCAGACAGTGACGGTGA	TGG	-0.698	151	197	-	175	6	18807323
+Cdc45	CRISPRko	ACTTCTCCAGACTCACCTTC	GGG	-0.5339	161	198	+	168	7	18805383
+Cdc45	CRISPRko	TACTGTCTTCATCAGGCTGA	AGG	-0.289	180	199	+	153	5	18808811
+Cdc45	CRISPRko	GTGCCTTTAGCTTTTTTGGT	CGG	-1.629	124	200	-	214	18	18784876
+Cdc45	CRISPRko	TTCTCGCAGATTCCCCTTCA	AGG	-1.122	133	201	+	205	13	18794808
+Cdc45	CRISPRko	ACAGAGGGTCCTTCTCTTTG	TGG	-0.6861	165	202	-	174	3	18811352
+Cdc45	CRISPRko	TGCCCTGTTCCACGCTCAGC	GGG	-0.7071	167	203	+	176	17	18785142
+Cdc45	CRISPRko	CTTGTGTCACTGCTGTCCTG	AGG	-0.08076	213	204	-	131	19	18781902
+Cdc45	CRISPRko	TTTCAGACAGTGACGGTGAT	GGG	-1.561	137	205	-	213	6	18807322
+Cdc45	CRISPRko	AAAAATATACTGTCTTCATC	AGG	-1.174	143	206	+	207	5	18808804
+Cdc45	CRISPRko	CAGCATGTCATTCAGGTCCT	TGG	-0.6403	179	207	+	172	9	18798695
+Cdc45	CRISPRko	GACTTGCTTCAGGGGAAGCC	TGG	-1.005	169	208	+	197	13	18794859
+Cdc45	CRISPRko	ACACCAGCTACACAGCAGCC	AGG	-0.3484	211	209	-	158	12	18795160
+Cdc45	CRISPRko	TTGTGTCACTGCTGTCCTGA	GGG	-0.8241	195	210	-	185	19	18781901
+Cdc45	CRISPRko	GGTCCTTCTCTTTGTGGCCT	CGG	-1.082	186	211	-	202	3	18811346
+Cdc45	CRISPRko	TTTCTGCTTGACTTGCTTCA	GGG	-1.255	185	212	+	210	13	18794850
+Cdc45	CRISPRko	AAACCTGGCTGCTGTGTAGC	TGG	-0.8948	212	213	+	191	12	18795168
+Cdc45	CRISPRko	CTCTGGGGGGATGCCCACCA	CGG	-1.018	206	214	+	198	17	18785113
+Cdc45	CRISPRko	ACGAGCAGTATGAATATTAT	GGG	-1.024	204	215	-	200	8	18801813
+Cdc45	CRISPRko	TGCCTTTAGCTTTTTTGGTC	GGG	-1.779	209	216	-	215	18	18784875
+Scml2	CRISPRko	AGGTTACGTCTACGACTGGA	TGG	1.541	3	1	+	5	4	161178165
+Scml2	CRISPRko	TCCACAGACAGCTCCAATTG	TGG	1.413	20	2	-	6	6	161192298
+Scml2	CRISPRko	CAGAAGAGGCGAATACCATG	GGG	1.167	18	3	+	11	2	161171514
+Scml2	CRISPRko	AATGATGCACTATTCACTGG	AGG	0.9748	14	4	-	17	24	161243407
+Scml2	CRISPRko	CATCTAATAAGACTTCTGCG	GGG	0.8661	10	5	+	23	22	161239834
+Scml2	CRISPRko	AGCCAGGTTACGTCTACGAC	TGG	0.7607	1	6	+	35	4	161178161
+Scml2	CRISPRko	TCTGTAAGGACCCTTCAACC	TGG	0.7828	7	7	+	33	26	161255095
+Scml2	CRISPRko	TAGTACACGTAGTAAGAACA	TGG	0.8471	16	8	-	27	5	161187297
+Scml2	CRISPRko	ACACCGAATCAATATTGCGA	GGG	1.595	39	9	-	4	4	161178099
+Scml2	CRISPRko	GCAAATGATGCACTATTCAC	TGG	0.7216	13	10	-	37	24	161243410
+Scml2	CRISPRko	CACACCGAATCAATATTGCG	AGG	1.22	40	11	-	10	4	161178100
+Scml2	CRISPRko	GCTGGAGAATGACATTCACA	GGG	0.9344	34	12	-	19	23	161242082
+Scml2	CRISPRko	ATAGACAAAGCAATCACCTG	GGG	0.7161	17	13	-	38	10	161213325
+Scml2	CRISPRko	ATCCAGTCGTAGACGTAACC	TGG	0.6009	2	14	-	53	4	161178152
+Scml2	CRISPRko	ATGGGGCAAACAGCGAATGA	AGG	0.5497	5	15	+	64	2	161171531
+Scml2	CRISPRko	AGAGATGTCCAAGTAGCCCC	AGG	1.055	63	16	+	12	11	161215259
+Scml2	CRISPRko	ATGATGCACTATTCACTGGA	GGG	1.351	72	17	-	9	24	161243406
+Scml2	CRISPRko	CTACCTAACCACTTCGGTCC	AGG	0.5316	28	18	+	66	23	161242070
+Scml2	CRISPRko	AGTACACGTAGTAAGAACAT	GGG	1.633	98	19	-	3	5	161187296
+Scml2	CRISPRko	TGAGGCGACACCATCCAACT	GGG	0.956	86	20	-	18	6	161192398
+Scml2	CRISPRko	CCTGTTGGGACGTGTGAACA	AGG	0.9842	88	21	+	16	4	161178240
+Scml2	CRISPRko	TGACCCTCGCAATATTGATT	CGG	0.3752	4	22	+	102	4	161178107
+Scml2	CRISPRko	TGTACTAACTGGATCTGAAC	TGG	0.3946	9	23	+	99	5	161187324
+Scml2	CRISPRko	AATCAATATTGCGAGGGTCA	CGG	0.4035	11	24	-	97	4	161178093
+Scml2	CRISPRko	GACCCCAGTGTCCTGAAACA	AGG	0.4732	35	25	+	81	26	161255070
+Scml2	CRISPRko	GTTGGATGGTGTCGCCTCAC	AGG	0.3861	19	26	+	100	6	161192413
+Scml2	CRISPRko	TATTGAGAGACAATCACCTG	GGG	0.7853	99	27	-	32	7	161202098
+Scml2	CRISPRko	GCCACAATTGGAGCTGTCTG	TGG	1.045	118	28	+	13	6	161192308
+Scml2	CRISPRko	AGACCTGGGAAACATGACCA	AGG	0.9065	111	29	+	22	10	161213311
+Scml2	CRISPRko	TTGATCAGTGCCTGTATCTG	GGG	0.5182	66	30	-	71	24	161243522
+Scml2	CRISPRko	CAGCAGCTACCTAACCACTT	CGG	0.2391	15	31	+	122	23	161242064
+Scml2	CRISPRko	AGAACTTTGAAAGATTGCCG	AGG	0.4136	46	32	+	95	8	161209262
+Scml2	CRISPRko	GTTGGGACGTGTGAACAAGG	AGG	1.798	139	33	+	2	4	161178243
+Scml2	CRISPRko	ACAAACAATGCAATCACCTG	GGG	0.3054	31	34	-	113	14	161224545
+Scml2	CRISPRko	ACAAACAATGCAATCACCTG	GGG	0.2833	30	35	-	116	17	161228761
+Scml2	CRISPRko	TGATTCGGTGTGTGTTGCTT	CGG	0.5176	74	36	+	72	4	161178122
+Scml2	CRISPRko	AAATAGTTGAAAAAAGACCA	AGG	0.8582	122	37	+	25	7	161202025
+Scml2	CRISPRko	GGAGATGTATTACAGCCACC	AGG	1.01	133	38	+	15	6	161192434
+Scml2	CRISPRko	CTTAAAACAGATAATAGAGG	AGG	0.857	127	39	+	26	23	161242172
+Scml2	CRISPRko	GTTGCCTGAAGAGGTCGGCG	AGG	0.5634	95	40	-	60	26	161255163
+Scml2	CRISPRko	CCTTGTTCACACGTCCCAAC	AGG	0.3007	41	41	-	114	4	161178229
+Scml2	CRISPRko	ACCTCCTTAAAGAACACTGC	AGG	0.7089	120	42	-	39	5	161187338
+Scml2	CRISPRko	AGTTTGAGCCCCTCTAGAGG	TGG	0.1481	22	43	-	139	21	161237579
+Scml2	CRISPRko	CATCAGACATACAACCTGTT	GGG	0.2252	45	44	+	124	4	161178226
+Scml2	CRISPRko	TCATCAGACATACAACCTGT	TGG	0.5606	107	45	+	62	4	161178225
+Scml2	CRISPRko	AAAGGTTGGAAAAAACCCAA	GGG	0.413	77	46	+	96	10	161213254
+Scml2	CRISPRko	AACAAAATCCACCTCTAGAG	GGG	0.03909	33	47	+	152	21	161237582
+Scml2	CRISPRko	GGGATCCTCAGTCCAAGGTG	TGG	0.7288	153	48	-	36	12	161221702
+Scml2	CRISPRko	TCCCGAGACATCTTCCCAGT	TGG	0.4559	104	49	+	86	6	161192395
+Scml2	CRISPRko	GGGATCCTCAGTCCAAGGTG	TGG	0.6335	147	50	-	45	18	161230127
+Scml2	CRISPRko	GGGATCCTCAGTCCAAGGTG	TGG	0.6373	148	51	-	44	17	161228713
+Scml2	CRISPRko	AAAGTAAGTACACTGGTCCC	AGG	0.4347	103	52	-	89	8	161209215
+Scml2	CRISPRko	GTGGCAGGACAGATCATAAA	TGG	0.1169	47	53	-	145	6	161192279
+Scml2	CRISPRko	GGGATCCTCAGTCCAAGGTG	TGG	0.6383	151	54	-	43	14	161224497
+Scml2	CRISPRko	AAAGTAAATGCACTGGTCCC	AGG	0.05065	44	55	-	150	9	161209629
+Scml2	CRISPRko	GGGCCTGGACCGAAGTGGTT	AGG	0.3554	90	56	-	106	23	161242062
+Scml2	CRISPRko	GGGATCCTCAGTCCAAGGTG	TGG	0.6292	150	57	-	46	15	161225913
+Scml2	CRISPRko	TCAGTGCCTGTATCTGGGGT	AGG	0.5054	125	58	-	75	24	161243518
+Scml2	CRISPRko	TGTTTATCCTTACCCAGTGG	AGG	1.381	195	59	-	7	4	161178268
+Scml2	CRISPRko	GGGATCCTCAGTCCAAGGTG	TGG	0.5945	149	60	-	54	16	161227409
+Scml2	CRISPRko	ATCTCCAAAAGTAAATGCAC	TGG	-0.07986	36	61	-	168	9	161209636
+Scml2	CRISPRko	CAAAGGTTGGAAAAAACCCA	AGG	0.17	68	62	+	138	10	161213253
+Scml2	CRISPRko	GGGATCCTCAGTCCAAGGTG	TGG	0.5942	152	63	-	55	13	161223193
+Scml2	CRISPRko	ACTTGAGCCTCACGTCTGAA	AGG	-0.003632	51	64	-	158	24	161243494
+Scml2	CRISPRko	GGGATCCTCAGTCCAAGGTG	TGG	0.5591	146	65	-	63	19	161231436
+Scml2	CRISPRko	ACCTGTCTGAAATATTCAGA	AGG	0.1938	80	66	-	133	3	161177303
+Scml2	CRISPRko	ACCTGCAGTGTTCTTTAAGG	AGG	0.6975	177	67	+	41	5	161187348
+Scml2	CRISPRko	GCAAAAGAAGAACCAGACTA	TGG	0.1371	78	68	+	143	7	161202049
+Scml2	CRISPRko	ACTTTGAAAGATTGCCGAGG	AGG	0.2174	94	69	+	128	8	161209265
+Scml2	CRISPRko	AAGAACAAAGCAATCACCTG	GGG	0.5621	163	70	-	61	19	161231484
+Scml2	CRISPRko	ATGAACTTTCCCATCAAAGA	AGG	-0.0688	65	71	-	165	24	161243372
+Scml2	CRISPRko	AAGAAATAGAAACACATCCT	TGG	0.2908	115	72	-	115	23	161242141
+Scml2	CRISPRko	CTGTGAATGTCATTCTCCAG	CGG	0.4281	142	73	+	90	23	161242095
+Scml2	CRISPRko	GGCAGAGGATTCATCAAACC	TGG	-0.5007	6	74	+	226	10	161213275
+Scml2	CRISPRko	CAGACAGCTCCAATTGTGGC	AGG	0.3795	132	75	-	101	6	161192294
+Scml2	CRISPRko	CAGAACAAAGCAATCACCTG	GGG	0.5289	168	76	-	67	12	161221750
+Scml2	CRISPRko	CAGAACAAAGCAATCACCTG	GGG	0.5246	166	77	-	70	15	161225961
+Scml2	CRISPRko	TAGAGAAAAACATTCACCTG	GGG	0.5378	172	78	-	65	11	161215264
+Scml2	CRISPRko	AAAACAGAACTATACCTCCT	CGG	-0.1327	61	79	-	177	8	161209268
+Scml2	CRISPRko	GACAAGTACTTGAAAGAGAC	TGG	-0.002056	82	80	+	156	3	161177278
+Scml2	CRISPRko	CAAGTTCCTACCCCAGATAC	AGG	0.08944	93	81	+	146	24	161243523
+Scml2	CRISPRko	GCTTCGGTCATTGGAATTAC	TGG	-0.3841	32	82	+	207	4	161178138
+Scml2	CRISPRko	AAGAACAAAGCAATCACCTG	GGG	0.5158	167	83	-	73	13	161223241
+Scml2	CRISPRko	GTGTGTGTTGCTTCGGTCAT	TGG	-0.4183	27	84	+	214	4	161178129
+Scml2	CRISPRko	AAGAACAAAGCAATCACCTG	GGG	0.4963	165	85	-	78	16	161227457
+Scml2	CRISPRko	CAGAGGATTCACCCAACCTT	TGG	-0.4886	21	86	+	222	11	161215216
+Scml2	CRISPRko	GCTGTAATACATCTCCTGTG	AGG	1.839	242	87	-	1	6	161192416
+Scml2	CRISPRko	AAGAACAAAGCAATCACCTG	GGG	0.4819	164	88	-	80	18	161230175
+Scml2	CRISPRko	TTCTTACTACGTGTACTAAC	TGG	-0.6431	8	89	+	238	5	161187313
+Scml2	CRISPRko	GCTGAACCTTCTGTGGATCC	AGG	0.5878	191	90	-	56	23	161242034
+Scml2	CRISPRko	GCAAAACAGTCACCACACCT	TGG	-0.2634	59	91	+	190	12	161221701
+Scml2	CRISPRko	TGCCTGAAGAGGTCGGCGAG	GGG	-0.04314	91	92	-	162	26	161255161
+Scml2	CRISPRko	GATTTACTTCAGCCTCCACT	GGG	0.623	208	93	+	48	4	161178267
+Scml2	CRISPRko	TTCATCTAATAAGACTTCTG	CGG	-0.287	64	94	+	193	22	161239832
+Scml2	CRISPRko	TCAAAAAGTTATCACACTGC	AGG	-0.2399	71	95	-	188	24	161243463
+Scml2	CRISPRko	ATCAATATTGCGAGGGTCAC	GGG	-0.07509	92	96	-	167	4	161178092
+Scml2	CRISPRko	CAAAGCTGAAAAAAAACCCA	AGG	0.1829	126	97	+	135	20	161234625
+Scml2	CRISPRko	AAGTCTGGGCAAGCGTCTAA	AGG	-0.4817	43	98	+	220	25	161246363
+Scml2	CRISPRko	GAGACAGGAGCAGAACGATG	AGG	0.8051	233	99	-	31	25	161246313
+Scml2	CRISPRko	GCAAAACAGTCACCACACCT	TGG	-0.4056	53	100	+	212	18	161230126
+Scml2	CRISPRko	GCAAAACAGTCACCACACCT	TGG	-0.4018	54	101	+	211	17	161228712
+Scml2	CRISPRko	GCAAAACAGTCACCACACCT	TGG	-0.3942	57	102	+	210	14	161224496
+Scml2	CRISPRko	TTGGTCATTTAGAGGCATTA	GGG	-0.7358	23	103	-	245	26	161255220
+Scml2	CRISPRko	GGCACTGATCAAAGCAAACC	AGG	0.05077	119	104	+	149	24	161243544
+Scml2	CRISPRko	GGAGTGGAGCATTTGATTAT	TGG	-0.7331	24	105	+	244	6	161192360
+Scml2	CRISPRko	ATGATCTGTCCTGCCACAAT	TGG	-0.1724	87	106	+	181	6	161192296
+Scml2	CRISPRko	TCATTGGAATTACTGGAGCC	AGG	0.5043	192	107	+	76	4	161178145
+Scml2	CRISPRko	GCAAAACAGTCACCACACCT	TGG	-0.4688	52	108	+	217	19	161231435
+Scml2	CRISPRko	GCAAAACAGTCACCACACCT	TGG	-0.4131	56	109	+	213	15	161225912
+Scml2	CRISPRko	GCAAAACAGTCACCACACCT	TGG	-0.439	55	110	+	215	16	161227408
+Scml2	CRISPRko	TAGCCAGCCTTTCAGACGTG	AGG	0.5029	197	111	+	77	24	161243498
+Scml2	CRISPRko	AAAACAAAATCCACCTCTAG	AGG	-0.7392	26	112	+	248	21	161237580
+Scml2	CRISPRko	GCAAAACAGTCACCACACCT	TGG	-0.4394	58	113	+	216	13	161223192
+Scml2	CRISPRko	CACAGGCCTGCACAGTCCGC	TGG	0.7737	241	114	-	34	23	161242100
+Scml2	CRISPRko	TTGTCCCAGTCAAAATGATC	TGG	-0.9279	12	115	-	263	3	161177249
+Scml2	CRISPRko	TCATCTAATAAGACTTCTGC	GGG	-0.5259	50	116	+	228	22	161239833
+Scml2	CRISPRko	AATACTTCATCCCCTAAGAA	GGG	-0.07423	112	117	+	166	21	161237697
+Scml2	CRISPRko	AATAGTAATGCCCTTCTTAG	GGG	-0.09467	106	118	-	173	21	161237696
+Scml2	CRISPRko	TTCATTCGCTGTTTGCCCCA	TGG	-0.2075	96	119	-	183	2	161171518
+Scml2	CRISPRko	TGGAAAAAACCCAAGGGCAG	AGG	-0.303	84	120	+	196	10	161213260
+Scml2	CRISPRko	GAGACATCTTCCCAGTTGGA	TGG	1.024	267	121	+	14	6	161192399
+Scml2	CRISPRko	GCTGATGAAGAAGCAATGCC	AGG	0.1391	143	122	+	141	20	161234692
+Scml2	CRISPRko	GAAAAGATGAAACCAGACCT	GGG	0.459	202	123	+	84	10	161213297
+Scml2	CRISPRko	CATCCACAGACCAGGTTGAA	GGG	-0.5301	60	124	-	229	26	161255094
+Scml2	CRISPRko	AAGAAGCTGCAGAAGAACCT	GGG	0.4397	203	125	+	88	9	161209623
+Scml2	CRISPRko	AAGAAGCTGCAGAAGAACCT	GGG	0.445	204	126	+	87	8	161209209
+Scml2	CRISPRko	AGAACTTTGAAAGATTGCCA	AGG	0.2809	176	127	+	117	9	161209676
+Scml2	CRISPRko	TCCAACTGGGAAGATGTCTC	GGG	0.6047	244	128	-	51	6	161192385
+Scml2	CRISPRko	TTTCTTAAAACAGATAATAG	AGG	-0.1308	121	129	+	176	23	161242169
+Scml2	CRISPRko	AGAGGAGGAGAAATGATAAC	TGG	0.4229	207	130	+	92	23	161242187
+Scml2	CRISPRko	GGACCCTTCAACCTGGTCTG	TGG	0.3676	199	131	+	103	26	161255102
+Scml2	CRISPRko	TACATGTTGCCTGAAGAGGT	CGG	0.819	275	132	-	30	26	161255168
+Scml2	CRISPRko	AAACACATCCTTGGCCTGAA	AGG	-0.2799	114	133	-	191	23	161242132
+Scml2	CRISPRko	TTGCCTGAAGAGGTCGGCGA	GGG	0.4854	227	134	-	79	26	161255162
+Scml2	CRISPRko	GATGCAGAGACAATCGTTTG	AGG	-0.4888	85	135	-	223	25	161246291
+Scml2	CRISPRko	AGTCACCACACCTTGGACTG	AGG	-0.009344	156	136	+	159	17	161228719
+Scml2	CRISPRko	ACTATGAAATTATTTTGGAG	TGG	-0.5826	81	137	-	234	6	161192222
+Scml2	CRISPRko	CTAAGGTTCCCAGAAAGTCT	GGG	-0.2866	124	138	+	192	25	161246349
+Scml2	CRISPRko	ATTCAAAATTGGTATGAAAT	TGG	-1.101	37	139	+	281	4	161178077
+Scml2	CRISPRko	AGTCACCACACCTTGGACTG	AGG	-0.01062	159	140	+	160	14	161224503
+Scml2	CRISPRko	CCAACTAATGAATTCAAAAT	TGG	-0.7435	70	141	+	250	4	161178066
+Scml2	CRISPRko	ACTTTGAAAGATTGCCAAGG	AGG	0.0711	173	142	+	148	9	161209679
+Scml2	CRISPRko	GCAGATCAAGGAGATGCCCC	AGG	0.643	280	143	+	42	13	161223236
+Scml2	CRISPRko	TATCACTTCATCCACAGACC	AGG	0.9277	306	144	-	20	26	161255102
+Scml2	CRISPRko	ATAAATAAACATGGAGATTG	TGG	0.3218	215	145	+	111	23	161242016
+Scml2	CRISPRko	AGTCACCACACCTTGGACTG	AGG	-0.08416	157	146	+	170	16	161227415
+Scml2	CRISPRko	GCAGATCAAGGAGATGCCCC	AGG	0.6265	281	147	+	47	12	161221745
+Scml2	CRISPRko	AGTCACCACACCTTGGACTG	AGG	-0.1076	154	148	+	175	19	161231442
+Scml2	CRISPRko	AGTCACCACACCTTGGACTG	AGG	-0.1034	155	149	+	174	18	161230133
+Scml2	CRISPRko	GCAGATCAAGGAGATGCCCC	AGG	0.605	279	150	+	50	15	161225956
+Scml2	CRISPRko	AGTCACCACACCTTGGACTG	AGG	-0.08449	158	151	+	171	15	161225919
+Scml2	CRISPRko	GCAGATCAAGGAGATGCCCC	AGG	0.6015	278	152	+	52	16	161227452
+Scml2	CRISPRko	AGTCACCACACCTTGGACTG	AGG	-0.0816	161	153	+	169	12	161221708
+Scml2	CRISPRko	AGTCACCACACCTTGGACTG	AGG	-0.08449	160	154	+	172	13	161223199
+Scml2	CRISPRko	GGAAATGAAGAAGAGGCCCC	AGG	0.6062	283	155	+	49	7	161202093
+Scml2	CRISPRko	TCCTTCTGAATATTTCAGAC	AGG	0.2609	212	156	+	120	3	161177313
+Scml2	CRISPRko	CCTTCTGTGGATCCAGGAAG	GGG	0.8626	309	157	-	24	23	161242028
+Scml2	CRISPRko	GCAGATCAAGGAGATGCCCC	AGG	0.5796	277	158	+	57	18	161230170
+Scml2	CRISPRko	GCAGATCAAGGAGATGCCCC	AGG	0.5638	276	159	+	59	19	161231479
+Scml2	CRISPRko	ATGTAACGTATCTTTTGATC	CGG	-1.187	42	160	+	294	26	161255197
+Scml2	CRISPRko	TCTAAGGTTCCCAGAAAGTC	TGG	-0.9778	67	161	+	270	25	161246348
+Scml2	CRISPRko	CATACTGATCCTCAAATATC	AGG	-0.7498	89	162	+	252	26	161255148
+Scml2	CRISPRko	GCAGGAAAATCTAAGTCTAC	TGG	-0.9541	75	163	+	266	21	161237673
+Scml2	CRISPRko	TGTGTGTATATAAATAAACA	TGG	0.8458	314	164	+	29	23	161242007
+Scml2	CRISPRko	TTTTCCAGATCATTTTGACT	GGG	-1.481	25	165	+	318	3	161177256
+Scml2	CRISPRko	CGCTGGAGAATGACATTCAC	AGG	-0.3895	136	166	-	208	23	161242083
+Scml2	CRISPRko	CTTCTTCATCAGCTAAAAAC	TGG	-1.193	48	167	-	296	20	161234671
+Scml2	CRISPRko	GCCTGAAGAGGTCGGCGAGG	GGG	0.7071	305	168	-	40	26	161255160
+Scml2	CRISPRko	TGGGTGAATCCTCTGCCCCT	GGG	-0.3624	140	169	-	205	11	161215197
+Scml2	CRISPRko	TCATCCACAGACCAGGTTGA	AGG	-0.3655	144	170	-	206	26	161255095
+Scml2	CRISPRko	TTTGATCAGTGCCTGTATCT	GGG	-0.7595	97	171	-	254	24	161243523
+Scml2	CRISPRko	TCACAGGGCCTGGACCGAAG	TGG	1.369	343	172	-	8	23	161242067
+Scml2	CRISPRko	TTGGTCATGTTTCCCAGGTC	TGG	-0.6896	109	173	-	242	10	161213298
+Scml2	CRISPRko	TTGATGAATCCTCTGCCCTT	GGG	-0.7379	105	174	-	247	10	161213258
+Scml2	CRISPRko	TTCCCAGAGGTAGAAGACTT	TGG	-1.132	69	175	-	284	21	161237548
+Scml2	CRISPRko	ACACACTGAATACTTCATCA	TGG	0.8471	325	176	+	28	5	161187284
+Scml2	CRISPRko	ATTGTCCTACCAAAGAAAAA	GGG	-1.07	76	177	+	278	21	161237733
+Scml2	CRISPRko	GCGAGGGGGCCTGATATTTG	AGG	-0.498	130	178	-	225	26	161255146
+Scml2	CRISPRko	AAGAAAAAGGGTGGAAAGTC	TGG	-1.052	79	179	+	277	21	161237745
+Scml2	CRISPRko	AGATTTACTTCAGCCTCCAC	TGG	0.2527	235	180	+	121	4	161178266
+Scml2	CRISPRko	GCAGATCAAGGAGCAGCCCC	AGG	0.5705	300	181	+	58	14	161224540
+Scml2	CRISPRko	ATTTTGAATTCATTAGTTGG	TGG	-0.7674	102	182	-	257	4	161178052
+Scml2	CRISPRko	AGTTATCACACTGCAGGCTT	TGG	-0.04523	200	183	-	163	24	161243457
+Scml2	CRISPRko	TGAAGTTTGAGCCCCTCTAG	AGG	-0.1927	181	184	-	182	21	161237582
+Scml2	CRISPRko	ATTGGTCATTTAGAGGCATT	AGG	-0.935	100	185	-	264	26	161255221
+Scml2	CRISPRko	ACGTTACATGTTGCCTGAAG	AGG	0.1953	232	186	-	132	26	161255172
+Scml2	CRISPRko	GTGAGGCGACACCATCCAAC	TGG	0.4677	285	187	-	82	6	161192399
+Scml2	CRISPRko	GCAGATCAAGGAGCAGCCCC	AGG	0.5265	299	188	+	69	17	161228756
+Scml2	CRISPRko	GGAAAAGATGAAACCAGACC	TGG	-0.2901	174	189	+	194	10	161213296
+Scml2	CRISPRko	AACTGTTTATCCTTACCCAG	TGG	0.9133	347	190	-	21	4	161178271
+Scml2	CRISPRko	CCTGCACATTGGTCATTTAG	AGG	-0.5705	137	191	-	232	26	161255228
+Scml2	CRISPRko	TTAATTAAAAAACAGAAAAT	GGG	-1.841	38	192	+	331	25	161246259
+Scml2	CRISPRko	TTGGAAGATGAAGCCAGACC	AGG	0.3992	271	193	+	98	11	161215235
+Scml2	CRISPRko	TTGGGTGAATCCTCTGCCCC	TGG	-0.3415	169	194	-	202	11	161215198
+Scml2	CRISPRko	GAGACTTAGAACTGCTCTTT	TGG	-2.094	29	195	+	342	7	161202071
+Scml2	CRISPRko	GTAGACATCACTATTTTAGA	TGG	-1.234	73	196	-	300	22	161239846
+Scml2	CRISPRko	ATCACTTTTGATGGATGGAG	TGG	-0.002087	216	197	+	157	6	161192344
+Scml2	CRISPRko	CATTCTCCAGCGGACTGTGC	AGG	0.1421	240	198	+	140	23	161242105
+Scml2	CRISPRko	CCAATTTTGAATTCATTAGT	TGG	-1.203	83	199	-	297	4	161178055
+Scml2	CRISPRko	GGGACCAGTGCATTTACTTT	TGG	-1.849	49	200	+	332	9	161209643
+Scml2	CRISPRko	AAACAAAATCCACCTCTAGA	GGG	-0.9577	116	201	+	267	21	161237581
+Scml2	CRISPRko	ACTTCAGCCTCCACTGGGTA	AGG	0.01336	229	202	+	154	4	161178272
+Scml2	CRISPRko	GGTAGCTGCTGAACCTTCTG	TGG	0.4595	301	203	-	83	23	161242041
+Scml2	CRISPRko	TTACAGCCACCAGGAAAAAT	TGG	-0.74	135	204	+	249	6	161192443
+Scml2	CRISPRko	GTCCTACCAAAGAAAAAGGG	TGG	0.1793	249	205	+	136	21	161237736
+Scml2	CRISPRko	TGGAGATTGTGGCCCCTTCC	TGG	-1.028	113	206	+	274	23	161242027
+Scml2	CRISPRko	TAATAGTAATGCCCTTCTTA	GGG	-0.9818	117	207	-	271	21	161237697
+Scml2	CRISPRko	GGTTGTTCAGAGGAAGAAGG	TGG	0.4584	303	208	-	85	21	161237527
+Scml2	CRISPRko	AAACATGACCAAGGAGCCCC	AGG	0.4155	296	209	+	94	10	161213320
+Scml2	CRISPRko	AGTTCTAAGTCTCCATAGTC	TGG	-0.8855	129	210	-	262	7	161202050
+Scml2	CRISPRko	GAAGATGTAGAAGAAATAAC	TGG	-0.4739	175	211	-	218	22	161239768
+Scml2	CRISPRko	TCTAAAATAGTGATGTCTAC	AGG	-0.8211	138	212	+	258	22	161239860
+Scml2	CRISPRko	CATCAGCTAAAAACTGGGCT	TGG	-1.041	123	213	-	276	20	161234665
+Scml2	CRISPRko	CTGAAAGGCACAATTGACAC	AGG	0.5283	333	214	-	68	23	161242117
+Scml2	CRISPRko	AATAAAAGCAATCACCTCCT	TGG	-0.7645	145	215	-	256	9	161209682
+Scml2	CRISPRko	TTATTTTGGAGTGGACGTGG	TGG	-0.06864	238	216	-	164	6	161192213
+Scml2	CRISPRko	AACCTTCTGTGGATCCAGGA	AGG	0.3303	295	217	-	110	23	161242030
+Scml2	CRISPRko	TTTTTCCAGATCATTTTGAC	TGG	-2.154	62	218	+	344	3	161177255
+Scml2	CRISPRko	TGTTTCAGGACACTGGGGTC	AGG	-0.3475	206	219	-	203	26	161255056
+Scml2	CRISPRko	AGTCTGGGATCCTCAGTCCA	AGG	0.2612	294	220	-	119	12	161221707
+Scml2	CRISPRko	ATCTCCAAAAGTAAGTACAC	TGG	0.1232	269	221	-	144	8	161209222
+Scml2	CRISPRko	AGTCTGGGATCCTCAGTCCA	AGG	0.2259	291	222	-	123	15	161225918
+Scml2	CRISPRko	AGTCTGGGATCCTCAGTCCA	AGG	0.2187	288	223	-	127	18	161230132
+Scml2	CRISPRko	AGTCTGGGATCCTCAGTCCA	AGG	0.2208	289	224	-	126	17	161228718
+Scml2	CRISPRko	CCTTACAGAAGCCTTGTTTC	AGG	-1.364	110	225	-	306	26	161255070
+Scml2	CRISPRko	AAAGCTGGGAAAAAACCCAG	GGG	0.5122	342	226	+	74	11	161215193
+Scml2	CRISPRko	GGCTTGGTCTTTCCGAGATT	TGG	-1.404	108	227	-	309	20	161234649
+Scml2	CRISPRko	AGTCTGGGATCCTCAGTCCA	AGG	0.2228	292	228	-	125	14	161224502
+Scml2	CRISPRko	AAAGCTGAGAAAAAACGCAA	GGG	0.4211	324	229	+	93	12	161221679
+Scml2	CRISPRko	GAGCCTCACGTCTGAAAGGC	TGG	0.01817	266	230	-	153	24	161243490
+Scml2	CRISPRko	AAAAGCTGGGAAAAAACCCA	GGG	0.0851	272	231	+	147	11	161215192
+Scml2	CRISPRko	AGTCTGGGATCCTCAGTCCA	AGG	0.2039	290	232	-	130	16	161227414
+Scml2	CRISPRko	TTGGACATCTCTTCCTGGTC	TGG	-0.831	162	233	-	259	11	161215237
+Scml2	CRISPRko	CTGTGTCAATTGTGCCTTTC	AGG	-1.533	101	234	+	324	23	161242129
+Scml2	CRISPRko	AAAGCTGAGAAAAAACGCAA	GGG	0.3442	319	235	+	107	17	161228690
+Scml2	CRISPRko	AAAGCTGAGAAAAAACGCAA	GGG	0.3629	322	236	+	104	14	161224474
+Scml2	CRISPRko	AAATACTTCATCCCCTAAGA	AGG	-0.5494	196	237	+	231	21	161237696
+Scml2	CRISPRko	AAAGCTGAGAAAAAACGCAA	GGG	0.3347	318	238	+	109	18	161230104
+Scml2	CRISPRko	AGTCTGGGATCCTCAGTCCA	AGG	0.1918	293	239	-	134	13	161223198
+Scml2	CRISPRko	AAAGCTGAGAAAAAACGCAA	GGG	0.3357	320	240	+	108	16	161227386
+Scml2	CRISPRko	AAAGCTGAGAAAAAACGCAA	GGG	0.3629	323	241	+	105	13	161223170
+Scml2	CRISPRko	AGAATGACATTCACAGGGCC	TGG	-0.4916	205	242	-	224	23	161242077
+Scml2	CRISPRko	AGTCTGGGATCCTCAGTCCA	AGG	0.1385	287	243	-	142	19	161231441
+Scml2	CRISPRko	AAATTATTTTGGAGTGGACG	TGG	-0.6655	190	244	-	240	6	161192216
+Scml2	CRISPRko	AAAGCTGAGAAAAAACGCAA	GGG	0.3181	321	245	+	112	15	161225890
+Scml2	CRISPRko	AAAGCTGAAAAAAAACCCAA	GGG	0.4229	344	246	+	91	20	161234626
+Scml2	CRISPRko	AAAGCTGAGAAAAAACGCAA	GGG	0.2637	317	247	+	118	19	161231413
+Scml2	CRISPRko	CAACCAAAGTCTTCTACCTC	TGG	-1.391	131	248	+	308	21	161237556
+Scml2	CRISPRko	GAATGATTTTTGGAGACTTG	TGG	-0.8636	178	249	+	261	4	161178200
+Scml2	CRISPRko	TGCACAACACTTGTTTTCCG	TGG	-1.317	141	250	-	303	21	161237614
+Scml2	CRISPRko	AAAAAGCTGGGAAAAAACCC	AGG	0.2115	316	251	+	129	11	161215191
+Scml2	CRISPRko	TAAAGCTGAGAAAAAACGCA	AGG	-0.2123	264	252	+	185	13	161223169
+Scml2	CRISPRko	CCAAAGACCAGCAAGAAAGC	AGG	-0.3613	246	253	+	204	21	161237655
+Scml2	CRISPRko	TAAAGCTGAGAAAAAACGCA	AGG	-0.2512	261	254	+	189	16	161227385
+Scml2	CRISPRko	TAAAGCTGAGAAAAAACGCA	AGG	-0.2328	265	255	+	187	12	161221678
+Scml2	CRISPRko	TGGCTTCATCTTCCAAAGGT	TGG	-0.6455	214	256	-	239	11	161215217
+Scml2	CRISPRko	ATTAGCTTACCAATTTTTCC	TGG	-1.483	134	257	-	319	6	161192441
+Scml2	CRISPRko	GGGACCAGTGTACTTACTTT	TGG	-1.569	128	258	+	326	8	161209229
+Scml2	CRISPRko	GGCACCTGCAGTGTTCTTTA	AGG	-1.14	171	259	+	285	5	161187345
+Scml2	CRISPRko	CTGGGAACCTTAGATGAGAC	AGG	-0.5218	230	260	-	227	25	161246328
+Scml2	CRISPRko	TAAAGCTGAGAAAAAACGCA	AGG	-0.3097	260	261	+	197	17	161228689
+Scml2	CRISPRko	TAAAGCTGAGAAAAAACGCA	AGG	-0.291	263	262	+	195	14	161224473
+Scml2	CRISPRko	GCTCCTTGGTCATGTTTCCC	AGG	-0.2088	274	263	-	184	10	161213303
+Scml2	CRISPRko	TAAAGCTGAGAAAAAACGCA	AGG	-0.3383	259	264	+	201	18	161230103
+Scml2	CRISPRko	GGGAAAAAACCCAGGGGCAG	AGG	-0.7118	218	265	+	243	11	161215199
+Scml2	CRISPRko	TAAAGCTGAGAAAAAACGCA	AGG	-0.3358	262	266	+	200	15	161225889
+Scml2	CRISPRko	CCCCTTCCTGGATCCACAGA	AGG	0.00568	308	267	+	155	23	161242039
+Scml2	CRISPRko	ACCTTCTGTGGATCCAGGAA	GGG	0.1753	328	268	-	137	23	161242029
+Scml2	CRISPRko	ACTTCATAGCCTTCTTTGAT	GGG	-0.7603	211	269	+	255	24	161243374
+Scml2	CRISPRko	TAAAGCTGAGAAAAAACGCA	AGG	-0.3902	258	270	+	209	19	161231412
+Scml2	CRISPRko	AGAAGACTTTGGTTGTTCAG	AGG	-0.3145	270	271	-	198	21	161237537
+Scml2	CRISPRko	TTTGGTTGTTCAGAGGAAGA	AGG	-0.482	247	272	-	221	21	161237530
+Scml2	CRISPRko	CCTTGATCTGCAAAAAGTCT	GGG	-1.111	187	273	-	282	14	161224517
+Scml2	CRISPRko	ATCCAACTGGGAAGATGTCT	CGG	0.1974	338	274	-	131	6	161192386
+Scml2	CRISPRko	CCTTGATCTGCAAAAAGTCT	GGG	-1.165	182	275	-	288	19	161231456
+Scml2	CRISPRko	CCTTGATCTGCAAAAAGTCT	GGG	-1.145	184	276	-	286	17	161228733
+Scml2	CRISPRko	CCTTGATCTGCAAAAAGTCT	GGG	-1.167	186	277	-	289	15	161225933
+Scml2	CRISPRko	CTAGAGGGGCTCAAACTTCA	AGG	-1.115	193	278	+	283	21	161237596
+Scml2	CRISPRko	CCTTGATCTGCAAAAAGTCT	GGG	-1.188	183	279	-	295	18	161230147
+Scml2	CRISPRko	CCTTGATCTGCAAAAAGTCT	GGG	-1.179	185	280	-	293	16	161227429
+Scml2	CRISPRko	CCTTGATCTGCAAAAAGTCT	GGG	-1.167	188	281	-	290	13	161223213
+Scml2	CRISPRko	CCTTGATCTGCAAAAAGTCT	GGG	-1.167	189	282	-	291	12	161221722
+Scml2	CRISPRko	GAAGCCTTGTTTCAGGACAC	TGG	-0.7498	236	283	-	251	26	161255063
+Scml2	CRISPRko	AGCCTTGTTTCAGGACACTG	GGG	0.04937	339	284	-	151	26	161255061
+Scml2	CRISPRko	AAACTTCAAGGAAGTCTCCA	CGG	-0.2132	304	285	+	186	21	161237608
+Scml2	CRISPRko	TTCATATCACTTTTGATGGA	TGG	-1.098	210	286	+	280	6	161192339
+Scml2	CRISPRko	GGTGGATTTTGTTTTCCCAG	AGG	-0.7377	248	287	-	246	21	161237561
+Scml2	CRISPRko	AACATTCACCTGGGGCTACT	TGG	-1.305	194	288	-	302	11	161215256
+Scml2	CRISPRko	GACGCTTGCCCAGACTTTCT	GGG	-1.221	201	289	-	299	25	161246346
+Scml2	CRISPRko	GCTACTTGGACATCTCTTCC	TGG	-0.5744	268	290	-	233	11	161215242
+Scml2	CRISPRko	AACCAAAGTCTTCTACCTCT	GGG	-0.9472	237	291	+	265	21	161237557
+Scml2	CRISPRko	CTTTGATCAGTGCCTGTATC	TGG	-1.528	180	292	-	323	24	161243524
+Scml2	CRISPRko	CCTGAAACAAGGCTTCTGTA	AGG	-0.02814	345	293	+	161	26	161255081
+Scml2	CRISPRko	TTTAGAGGCATTAGGGCAGC	AGG	-0.9685	239	294	-	269	26	161255213
+Scml2	CRISPRko	GGTCTGGCTTCATCTTCCAA	AGG	-0.138	332	295	-	178	11	161215221
+Scml2	CRISPRko	CAAGAAGCTGCAGAAGAACC	TGG	-0.149	330	296	+	180	9	161209622
+Scml2	CRISPRko	CAAGAAGCTGCAGAAGAACC	TGG	-0.1437	331	297	+	179	8	161209208
+Scml2	CRISPRko	TTCTTGCTGGTCTTTGGTAC	TGG	-1.326	209	298	-	305	21	161237638
+Scml2	CRISPRko	TTCCACCCTTTTTCTTTGGT	AGG	-1.964	179	299	-	338	21	161237727
+Scml2	CRISPRko	AGACTTAGAACTGCTCTTTT	GGG	-2.336	170	300	+	347	7	161202072
+Scml2	CRISPRko	AATTGTCCTACCAAAGAAAA	AGG	-1.416	213	301	+	310	21	161237732
+Scml2	CRISPRko	TTTGGTGACTCTTTTGCCCT	TGG	-1.673	198	302	-	327	20	161234631
+Scml2	CRISPRko	CAATTGTGCCTTTCAGGCCA	AGG	-0.4747	310	303	+	219	23	161242135
+Scml2	CRISPRko	TCCTTGATCTGCAAAAAGTC	TGG	-1.429	219	304	-	312	19	161231457
+Scml2	CRISPRko	TCCTTGATCTGCAAAAAGTC	TGG	-1.385	224	305	-	307	14	161224518
+Scml2	CRISPRko	AAGCCTTGTTTCAGGACACT	GGG	-1.208	234	306	-	298	26	161255062
+Scml2	CRISPRko	TCCTTGATCTGCAAAAAGTC	TGG	-1.418	221	307	-	311	17	161228734
+Scml2	CRISPRko	TCCTTGATCTGCAAAAAGTC	TGG	-1.43	223	308	-	313	15	161225934
+Scml2	CRISPRko	TCCTTGATCTGCAAAAAGTC	TGG	-1.451	220	309	-	317	18	161230148
+Scml2	CRISPRko	TCCTTGATCTGCAAAAAGTC	TGG	-1.442	222	310	-	316	16	161227430
+Scml2	CRISPRko	TCCTTGATCTGCAAAAAGTC	TGG	-1.43	225	311	-	314	13	161223214
+Scml2	CRISPRko	TAATTAAAAAACAGAAAATG	GGG	-0.3324	341	312	+	199	25	161246260
+Scml2	CRISPRko	TCCTTGATCTGCAAAAAGTC	TGG	-1.43	226	313	-	315	12	161221723
+Scml2	CRISPRko	AGCAATCACCTGGGGCTCCT	TGG	-1.317	243	314	-	304	10	161213317
+Scml2	CRISPRko	GCCCCCTCGCCGACCTCTTC	AGG	-1.032	273	315	+	275	26	161255170
+Scml2	CRISPRko	GCAAAAGAGTCACCAAATCT	CGG	-0.6335	312	316	+	237	20	161234648
+Scml2	CRISPRko	GGGTGGAAAGTCTGGAAAAA	AGG	-0.9654	282	317	+	268	21	161237753
+Scml2	CRISPRko	TCTTTTGGGAAATGAAGAAG	AGG	-0.5431	329	318	+	230	7	161202086
+Scml2	CRISPRko	GACTTTCCACCCTTTTTCTT	TGG	-2.108	217	319	-	343	21	161237731
+Scml2	CRISPRko	TTTGATGAATCCTCTGCCCT	TGG	-0.6303	326	320	-	236	10	161213259
+Scml2	CRISPRko	AGCTTACCAATTTTTCCTGG	TGG	-1.074	284	321	-	279	6	161192438
+Scml2	CRISPRko	TTGGTGACTCTTTTGCCCTT	GGG	-1.877	231	322	-	334	20	161234630
+Scml2	CRISPRko	TTCTGCTCCTGTCTCATCTA	AGG	-0.9905	298	323	+	272	25	161246332
+Scml2	CRISPRko	GGCTTCATCTTCCAAAGGTT	GGG	-0.5908	335	324	-	235	11	161215216
+Scml2	CRISPRko	CCTGCTTTCTTGCTGGTCTT	TGG	-1.754	245	325	-	328	21	161237644
+Scml2	CRISPRko	TTCTTCATCAGCTAAAAACT	GGG	-0.8375	315	326	-	260	20	161234670
+Scml2	CRISPRko	AGACGCTTGCCCAGACTTTC	TGG	-2.466	228	327	-	349	25	161246347
+Scml2	CRISPRko	AACAGAAAATGGGGAACCAA	AGG	-0.6712	337	328	+	241	25	161246269
+Scml2	CRISPRko	CATTAGGGCAGCAGGAAAAC	CGG	-0.7586	327	329	-	253	26	161255205
+Scml2	CRISPRko	CCCAGACTTTTTGCAGATCA	AGG	-1.86	252	330	+	333	17	161228744
+Scml2	CRISPRko	CCCAGACTTTTTGCAGATCA	AGG	-1.809	255	331	+	330	14	161224528
+Scml2	CRISPRko	CCCAGACTTTTTGCAGATCA	AGG	-1.991	250	332	+	341	19	161231467
+Scml2	CRISPRko	CCCAGACTTTTTGCAGATCA	AGG	-1.982	251	333	+	340	18	161230158
+Scml2	CRISPRko	CCCAGACTTTTTGCAGATCA	AGG	-1.954	254	334	+	337	15	161225944
+Scml2	CRISPRko	CCCAGACTTTTTGCAGATCA	AGG	-1.939	256	335	+	335	13	161223224
+Scml2	CRISPRko	CCCAGACTTTTTGCAGATCA	AGG	-1.965	253	336	+	339	16	161227440
+Scml2	CRISPRko	CCCAGACTTTTTGCAGATCA	AGG	-1.952	257	337	+	336	12	161221733
+Scml2	CRISPRko	TACTTCATAGCCTTCTTTGA	TGG	-1.269	297	338	+	301	24	161243373
+Scml2	CRISPRko	TTAATAGTAATGCCCTTCTT	AGG	-1.174	313	339	-	292	21	161237698
+Scml2	CRISPRko	GTGACAATAAGAATGATTTT	TGG	-1.011	340	340	+	273	4	161178190
+Scml2	CRISPRko	CTCCAAAATAATTTCATAGT	TGG	-1.155	334	341	+	287	6	161192236
+Scml2	CRISPRko	CTGGTTCTTCTTTTGCGCCT	TGG	-2.187	286	342	-	345	7	161202031
+Scml2	CRISPRko	CAGGTTCTTCTGCAGCTTCT	TGG	-1.528	311	343	-	322	9	161209610
+Scml2	CRISPRko	TTGAGGCTTCTCTTTTCCTT	TGG	-2.429	302	344	-	348	25	161246274
+Scml2	CRISPRko	AGATTTTCCTGCTTTCTTGC	TGG	-2.237	307	345	-	346	21	161237651
+Scml2	CRISPRko	CAACTTCATATCACTTTTGA	TGG	-1.506	336	346	+	321	6	161192335
+Scml2	CRISPRko	TCCCAACTATGAAATTATTT	TGG	-1.497	346	347	-	320	6	161192227
+Scml2	CRISPRko	TCCAAAATAATTTCATAGTT	GGG	-1.541	350	348	+	325	6	161192237
+Scml2	CRISPRko	GGTCTGGTTTCATCTTTTCC	AGG	-1.762	348	349	-	329	10	161213282
+Scml2	CRISPRko	TTTAATTAAAAAACAGAAAA	TGG	-2.657	349	350	+	350	25	161246258
+Apoh	CRISPRko	AAAGACATCCTACGACCCTG	GGG	0.9011	4	1	+	18	2	108395879
+Apoh	CRISPRko	AGTAGACAATCTGCTCCCCA	GGG	1.227	22	2	-	7	2	108395883
+Apoh	CRISPRko	GGATGACCTACCATTTGCTA	CGG	0.7778	9	3	+	21	2	108395846
+Apoh	CRISPRko	TTAAAGACATCCTACGACCC	TGG	0.7034	1	4	+	30	2	108395877
+Apoh	CRISPRko	AGGATTATAGGCCTTCAGCT	GGG	0.7455	17	5	+	24	5	108407353
+Apoh	CRISPRko	TCCAGCGAAAGGACATACTC	TGG	0.5591	10	6	-	41	3	108397305
+Apoh	CRISPRko	AAGGGGACAACCGTAGCAAA	TGG	0.7155	23	7	-	28	2	108395845
+Apoh	CRISPRko	CAGCTCATCTAAGTGCACGG	AGG	1.705	52	8	+	1	4	108404846
+Apoh	CRISPRko	GGACAACCGTAGCAAATGGT	AGG	1.269	49	9	-	6	2	108395841
+Apoh	CRISPRko	GGTCCCATTCAGAAAAAACC	TGG	0.4673	15	10	-	46	4	108404813
+Apoh	CRISPRko	TGTCTCATGGCAACCAAATG	TGG	0.6828	30	11	-	33	6	108409221
+Apoh	CRISPRko	TTTAAGAATGGGATGATGCA	TGG	0.5341	20	12	+	44	7	108412083
+Apoh	CRISPRko	TTTCCGATCATGGCAAAGTG	TGG	0.3519	6	13	-	58	5	108407407
+Apoh	CRISPRko	TCCTTCTTGCCGACCTGTAG	AGG	0.5166	26	14	+	45	6	108409306
+Apoh	CRISPRko	GGTCGTAGGATGTCTTTAAG	GGG	0.5445	28	15	-	43	2	108395862
+Apoh	CRISPRko	TTATCCTTATATAGAAGCAC	CGG	0.715	43	16	-	29	6	108409196
+Apoh	CRISPRko	CTCACTACAATCCTTACCCA	GGG	0.8856	54	17	-	19	3	108397396
+Apoh	CRISPRko	AATGTACCAAGACGGGAACT	TGG	0.2665	5	18	+	69	6	108409283
+Apoh	CRISPRko	GGAGAACAAGGCGAGCACCG	GGG	1.66	73	19	-	2	1	108395357
+Apoh	CRISPRko	CATCCCTTGGTACAGCACGG	TGG	1.105	72	20	-	9	7	108412028
+Apoh	CRISPRko	GAGCACCGGGGAAACCATTG	CGG	1.044	68	21	-	13	1	108395345
+Apoh	CRISPRko	AAGCCACCGTGCTGTACCAA	GGG	0.6211	50	22	+	37	7	108412036
+Apoh	CRISPRko	AGAAGCGGAATGTACCAAGA	CGG	0.7177	60	23	+	27	6	108409275
+Apoh	CRISPRko	TAAAGACATCCTACGACCCT	GGG	0.01916	3	24	+	85	2	108395878
+Apoh	CRISPRko	GACCAGCTCATCTAAGTGCA	CGG	1.068	79	25	+	10	4	108404843
+Apoh	CRISPRko	GTAGCAAATGGTAGGTCATC	CGG	0.3899	37	26	-	52	2	108395833
+Apoh	CRISPRko	AAAGCCACCGTGCTGTACCA	AGG	0.8319	71	27	+	20	7	108412035
+Apoh	CRISPRko	CAAGACTCACGAGCACAAGC	AGG	0.3548	34	28	-	57	4	108404881
+Apoh	CRISPRko	CAAGCCAGGCTACGTGTCCA	GGG	0.3567	35	29	+	56	2	108395921
+Apoh	CRISPRko	AATGGTAGGTCATCCGGCTT	CGG	0.283	25	30	-	67	2	108395827
+Apoh	CRISPRko	ACCTCTACAGGTCGGCAAGA	AGG	0.382	41	31	-	53	6	108409296
+Apoh	CRISPRko	GAAGCGGAATGTACCAAGAC	GGG	0.2587	24	32	+	72	6	108409276
+Apoh	CRISPRko	GCCAGAGTATGTCCTTTCGC	TGG	-0.02176	7	33	+	89	3	108397315
+Apoh	CRISPRko	AAGCCAGGCTACGTGTCCAG	GGG	1.364	91	34	+	5	2	108395922
+Apoh	CRISPRko	GAAGAAGTGCAGCTACACTG	TGG	1.536	99	35	+	3	7	108412139
+Apoh	CRISPRko	AAACCGTCTCATCCCTCCCC	TGG	0.9311	85	36	-	17	2	108395927
+Apoh	CRISPRko	GCGGAGAACAAGGCGAGCAC	CGG	0.3051	38	37	-	64	1	108395359
+Apoh	CRISPRko	GCCTTACCCTTGAAGCACGA	GGG	0.9759	87	38	-	16	7	108412183
+Apoh	CRISPRko	AGGAGTGCAAACTTTGGAAC	TGG	0.002833	16	39	-	87	5	108407317
+Apoh	CRISPRko	ACAGTCATGTGCACAGAACA	AGG	0.6983	77	40	+	31	5	108407445
+Apoh	CRISPRko	CCGTGCTGTACCAAGGGATG	AGG	0.2938	45	41	+	66	7	108412042
+Apoh	CRISPRko	ACACATCTCAGGGTGTTGAT	GGG	-0.072	19	42	-	94	2	108395974
+Apoh	CRISPRko	GACCCGATTGCCAGAATGCC	TGG	-0.2394	14	43	+	101	5	108407474
+Apoh	CRISPRko	TCATCTAAGTGCACGGAGGA	AGG	-0.3414	13	44	+	103	4	108404850
+Apoh	CRISPRko	CAGACTTACGGACACATCTC	AGG	0.1862	36	45	-	81	2	108395985
+Apoh	CRISPRko	CCTGTCCTCTCACAGGAATG	TGG	0.223	42	46	+	76	2	108395962
+Apoh	CRISPRko	ATTTTCTGGCCTCGGAGGGA	AGG	-0.1792	21	47	-	98	6	108409149
+Apoh	CRISPRko	GAAGGACCAAGTTCCCGTCT	TGG	0.0002718	32	48	-	88	6	108409278
+Apoh	CRISPRko	AGCCTTACCCTTGAAGCACG	AGG	0.7655	100	49	-	22	7	108412184
+Apoh	CRISPRko	TTGCCATGAGACATACAAGC	TGG	0.3403	62	50	+	60	6	108409242
+Apoh	CRISPRko	AGACTTACGGACACATCTCA	GGG	0.6863	90	51	-	32	2	108395984
+Apoh	CRISPRko	AAAGCCGGTGCTTCTATATA	AGG	-0.6612	2	52	+	121	6	108409203
+Apoh	CRISPRko	ACCCGATTGCCAGAATGCCT	GGG	0.4142	74	53	+	49	5	108407475
+Apoh	CRISPRko	AAAGAGTTGTTCCCAGCTGA	AGG	0.2087	46	54	-	78	5	108407353
+Apoh	CRISPRko	GGGTCGTAGGATGTCTTTAA	GGG	-0.6385	8	55	-	119	2	108395863
+Apoh	CRISPRko	GCACGGTGGCTTTCTTAACG	GGG	0.5497	86	56	-	42	7	108412014
+Apoh	CRISPRko	CGCATCCGCAATGGTTTCCC	CGG	0.2534	56	57	+	73	1	108395351
+Apoh	CRISPRko	GAAGTGCAGCTACACTGTGG	AGG	1.155	124	58	+	8	7	108412142
+Apoh	CRISPRko	GCAGAGGAAGGCGGAGAACA	AGG	1.025	120	59	-	14	1	108395369
+Apoh	CRISPRko	GAGATTCCCTCGTGCTTCAA	GGG	-0.317	33	60	+	102	7	108412188
+Apoh	CRISPRko	GTGGAGGCTCATTGCAGAGA	TGG	0.6803	103	61	+	34	7	108412158
+Apoh	CRISPRko	GTGTTCAGTCTGTCCGAAGC	CGG	0.7625	114	62	+	23	2	108395825
+Apoh	CRISPRko	TCCCTCGTGCTTCAAGGGTA	AGG	0.3106	75	63	+	63	7	108412193
+Apoh	CRISPRko	GCTGGACGGCCCAGAAGAAG	CGG	1.05	126	64	+	12	6	108409260
+Apoh	CRISPRko	TCAGTTTTGCTTGTAACCCT	GGG	-0.6436	18	65	+	120	3	108397391
+Apoh	CRISPRko	TCCCTCCGAGGCCAGAAAAT	GGG	0.3513	80	66	+	59	6	108409163
+Apoh	CRISPRko	TTGCCACACTTTGCCATGAT	CGG	-0.9985	12	67	+	127	5	108407415
+Apoh	CRISPRko	CTGAAGGCCTATAATCCTTA	AGG	-1.046	11	68	-	128	5	108407337
+Apoh	CRISPRko	ATGCCAGTACCTCTACAGGT	CGG	1.368	136	69	-	4	6	108409304
+Apoh	CRISPRko	CATGAGACATACAAGCTGGA	CGG	0.7442	116	70	+	25	6	108409246
+Apoh	CRISPRko	TGTGCACAGAACAAGGAAAC	TGG	0.2404	66	71	+	75	5	108407452
+Apoh	CRISPRko	GCAAGCCAGGCTACGTGTCC	AGG	0.01754	55	72	+	86	2	108395920
+Apoh	CRISPRko	GAGTAGACAATCTGCTCCCC	AGG	0.184	61	73	-	82	2	108395884
+Apoh	CRISPRko	CCTCATCCCTTGGTACAGCA	CGG	0.6094	107	74	-	38	7	108412031
+Apoh	CRISPRko	TGTGAATTATCCTGCAAAGC	CGG	-0.04904	58	75	+	90	6	108409188
+Apoh	CRISPRko	GCCATGTTGCTATTGCAGGA	CGG	0.9798	135	76	+	15	1	108395401
+Apoh	CRISPRko	GAGCACAAGCAGGAATATCT	GGG	0.2643	81	77	-	70	4	108404871
+Apoh	CRISPRko	TATAAGGATAAAGCCACATT	TGG	-0.1051	59	78	+	95	6	108409219
+Apoh	CRISPRko	TCCGTCCTGCAATAGCAACA	TGG	1.053	143	79	-	11	1	108395391
+Apoh	CRISPRko	CAGGCTACGTGTCCAGGGGA	GGG	0.3753	102	80	+	54	2	108395926
+Apoh	CRISPRko	AGCACGGTGGCTTTCTTAAC	GGG	-0.9946	31	81	-	126	7	108412015
+Apoh	CRISPRko	TGGGAACAACTCTTTGTATC	AGG	-1.064	27	82	+	131	5	108407372
+Apoh	CRISPRko	AATCTGCTCCCCAGGGTCGT	AGG	0.4057	108	83	-	50	2	108395876
+Apoh	CRISPRko	CGTGCTGTACCAAGGGATGA	GGG	0.7235	133	84	+	26	7	108412043
+Apoh	CRISPRko	TTTTCTAAGATTCCAGCGAA	AGG	-1.061	29	85	-	130	3	108397316
+Apoh	CRISPRko	AGCAACATGGCAGAGGAAGG	CGG	0.6263	123	86	-	36	1	108395378
+Apoh	CRISPRko	CGGAGAACAAGGCGAGCACC	GGG	0.3199	98	87	-	61	1	108395358
+Apoh	CRISPRko	CTGCAATAGCAACATGGCAG	AGG	0.2127	83	88	-	77	1	108395385
+Apoh	CRISPRko	CGAGCACAAGCAGGAATATC	TGG	-0.6701	39	89	-	122	4	108404872
+Apoh	CRISPRko	TGTCCAGGGGAGGGATGAGA	CGG	0.5607	121	90	+	40	2	108395935
+Apoh	CRISPRko	GACTGTGTCATTTCCGATCA	TGG	-0.4829	48	91	-	114	5	108407417
+Apoh	CRISPRko	CCAGGCTACGTGTCCAGGGG	AGG	-0.1428	67	92	+	96	2	108395925
+Apoh	CRISPRko	CGTCCAGCTTGTATGTCTCA	TGG	-0.5085	47	93	-	117	6	108409234
+Apoh	CRISPRko	ATGGGCCACATTCCTGTGAG	AGG	0.6566	130	94	-	35	2	108395956
+Apoh	CRISPRko	AGGGATGAGGGTGAAGATCC	AGG	0.5666	127	95	+	39	7	108412055
+Apoh	CRISPRko	TAAAATGTCCCTTCCCTCCG	AGG	0.4572	119	96	+	47	6	108409151
+Apoh	CRISPRko	TTTGCTTGTAACCCTGGGTA	AGG	-0.06387	78	97	+	91	3	108397396
+Apoh	CRISPRko	TTCTGGAAACGACTTCAGCA	CGG	0.189	92	98	-	79	8	108414293
+Apoh	CRISPRko	TATAGAAGCACCGGCTTTGC	AGG	-0.4988	57	99	-	115	6	108409187
+Apoh	CRISPRko	AGTGCAAACTTTGGAACTGG	TGG	0.294	109	100	-	65	5	108407314
+Apoh	CRISPRko	CGGTTTACCTGTCCTCTCAC	AGG	0.3117	113	101	+	62	2	108395955
+Apoh	CRISPRko	AATAGCAACATGGCAGAGGA	AGG	0.2601	105	102	-	71	1	108395381
+Apoh	CRISPRko	TGGTACATTCCGCTTCTTCT	GGG	-0.4238	69	103	-	108	6	108409258
+Apoh	CRISPRko	GACACATCTCAGGGTGTTGA	TGG	-0.4677	65	104	-	113	2	108395975
+Apoh	CRISPRko	TACCCAGGCATTCTGGCAAT	CGG	-0.5579	64	105	-	118	5	108407466
+Apoh	CRISPRko	TAATTCACATACCCATTTTC	TGG	-1.238	44	106	-	140	6	108409163
+Apoh	CRISPRko	TTCGCTGGAATCTTAGAAAA	TGG	-0.2358	84	107	+	100	3	108397330
+Apoh	CRISPRko	CCACATTCCTGTGAGAGGAC	AGG	0.2428	111	108	-	74	2	108395951
+Apoh	CRISPRko	TCCAAAGTTTGCACTCCTTA	AGG	-2.006	40	109	+	146	5	108407333
+Apoh	CRISPRko	ACTTTGGAACTGGTGGTGGC	GGG	0.37	131	110	-	55	5	108407307
+Apoh	CRISPRko	ATTGTCTACTCCTGCAAGCC	AGG	-0.4628	76	111	+	111	2	108395907
+Apoh	CRISPRko	CTCTTTGTATCAGGACACAG	TGG	0.4171	144	112	+	48	5	108407381
+Apoh	CRISPRko	CCTCCCCTGGACACGTAGCC	TGG	-1.255	53	113	-	141	2	108395914
+Apoh	CRISPRko	CCACATGCCAGTACCTCTAC	AGG	-0.4148	88	114	-	107	6	108409308
+Apoh	CRISPRko	TTGCACTCCTTAAGGATTAT	AGG	-1.41	51	115	+	144	5	108407341
+Apoh	CRISPRko	AGCCAGAGAACTGTGCTCTG	TGG	0.3944	147	116	-	51	8	108414240
+Apoh	CRISPRko	TGGAACTGGTGGTGGCGGGC	AGG	-0.364	94	117	-	104	5	108407303
+Apoh	CRISPRko	TCCCATTCTTAAACTGTTCC	TGG	-1.199	63	118	-	139	7	108412062
+Apoh	CRISPRko	CTCCGTGCACTTAGATGAGC	TGG	-1.088	70	119	-	132	4	108404834
+Apoh	CRISPRko	TCCCACAGAGCACAGTTCTC	TGG	-0.2061	104	120	+	99	8	108414249
+Apoh	CRISPRko	GGATCTTCACCCTCATCCCT	TGG	0.1405	128	121	-	83	7	108412041
+Apoh	CRISPRko	AAGGATTATAGGCCTTCAGC	TGG	-0.1462	115	122	+	97	5	108407352
+Apoh	CRISPRko	AGTGCACGGAGGAAGGAAAA	TGG	0.2789	146	123	+	68	4	108404857
+Apoh	CRISPRko	GCAAACTTTGGAACTGGTGG	TGG	0.1879	138	124	-	80	5	108407311
+Apoh	CRISPRko	ACAAGCAAAACTGATGTTCT	TGG	-1.155	82	125	-	137	3	108397371
+Apoh	CRISPRko	TCCAGGAACAGTTTAAGAAT	GGG	0.02718	137	126	+	84	7	108412072
+Apoh	CRISPRko	AACTTTGGAACTGGTGGTGG	CGG	-0.06445	129	127	-	92	5	108407308
+Apoh	CRISPRko	CAGCACGGTGGCTTTCTTAA	CGG	-1.058	93	128	-	129	7	108412016
+Apoh	CRISPRko	ACCCATTTTCTGGCCTCGGA	GGG	-0.5037	106	129	-	116	6	108409153
+Apoh	CRISPRko	AGCCACTTACCCAGGCATTC	TGG	-0.4015	118	130	-	105	5	108407473
+Apoh	CRISPRko	GGACACGTAGCCTGGCTTGC	AGG	-0.4479	117	131	-	110	2	108395906
+Apoh	CRISPRko	ATCAGTTTTGCTTGTAACCC	TGG	-1.12	96	132	+	135	3	108397390
+Apoh	CRISPRko	CGAGATTCCCTCGTGCTTCA	AGG	-1.364	89	133	+	143	7	108412187
+Apoh	CRISPRko	TTGGTACATTCCGCTTCTTC	TGG	-1.197	97	134	-	138	6	108409259
+Apoh	CRISPRko	CAAGCAAAACTGATGTTCTT	GGG	-1.277	95	135	-	142	3	108397370
+Apoh	CRISPRko	CTCTGCCATGTTGCTATTGC	AGG	-1.136	101	136	+	136	1	108395397
+Apoh	CRISPRko	TTCCCTCCGAGGCCAGAAAA	TGG	-0.06838	145	137	+	93	6	108409162
+Apoh	CRISPRko	TCCTTAAGGAGTGCAAACTT	TGG	-0.4461	132	138	-	109	5	108407323
+Apoh	CRISPRko	ACATACCCATTTTCTGGCCT	CGG	-1.113	110	139	-	134	6	108409157
+Apoh	CRISPRko	TTTTCTGGCCTCGGAGGGAA	GGG	-0.4126	139	140	-	106	6	108409148
+Apoh	CRISPRko	TTCTCTGGCTTTCTGGAAAA	CGG	-0.9146	125	141	+	125	8	108414264
+Apoh	CRISPRko	TACCCATTTTCTGGCCTCGG	AGG	-0.4662	140	142	-	112	6	108409154
+Apoh	CRISPRko	ACCCAGGCATTCTGGCAATC	GGG	-0.756	134	143	-	123	5	108407465
+Apoh	CRISPRko	TTTCCAGGTTTTTTCTGAAT	GGG	-2.006	112	144	+	147	4	108404821
+Apoh	CRISPRko	AGCACAGTTCTCTGGCTTTC	TGG	-2.148	122	145	+	148	8	108414257
+Apoh	CRISPRko	ATCCAGGAACAGTTTAAGAA	TGG	-0.8181	148	146	+	124	7	108412071
+Apoh	CRISPRko	AGGGTCGTAGGATGTCTTTA	AGG	-1.102	142	147	-	133	2	108395864
+Apoh	CRISPRko	TTTTCCAGGTTTTTTCTGAA	TGG	-1.996	141	148	+	145	4	108404820
+Narf	CRISPRko	AGCAAGGAGCCACGACAACA	TGG	0.8793	4	1	-	27	7	121246955
+Narf	CRISPRko	GAGGTGCTCGCGTGTCCCAG	AGG	1.045	15	2	+	17	10	121250913
+Narf	CRISPRko	GTGTCACTGATGCATCTAGA	AGG	0.8673	9	3	+	29	4	121244696
+Narf	CRISPRko	AGATCACCTACCGTGCACTC	AGG	0.6391	7	4	+	40	9	121250426
+Narf	CRISPRko	GAGCACTGCACACGAAAGGT	GGG	0.788	13	5	+	34	1	121237351
+Narf	CRISPRko	TAGGAACAAAGACTTCCATG	AGG	1.435	45	6	+	4	10	121250774
+Narf	CRISPRko	CAAATGGAAGGGATCTACTC	CGG	0.5447	5	7	+	45	11	121252763
+Narf	CRISPRko	GCAGCAGTGCACGATCTGTG	TGG	1.424	52	8	-	5	11	121252727
+Narf	CRISPRko	TACCGCCAAGTCCCCCCAGC	AGG	0.419	3	9	+	57	6	121245410
+Narf	CRISPRko	GGCAAAGTAATCCTTCACCA	AGG	1.531	65	10	-	2	6	121245431
+Narf	CRISPRko	ACTCCCATACCACTTTGTGG	AGG	1.135	55	11	+	15	10	121250894
+Narf	CRISPRko	TAGATGCATCAGTGACACTG	AGG	1.481	68	12	-	3	4	121244680
+Narf	CRISPRko	ATGATGGAGTGAGTTCAGAT	GGG	0.5058	26	13	+	49	9	121250345
+Narf	CRISPRko	TGAGCACTGCACACGAAAGG	TGG	0.5435	32	14	+	46	1	121237350
+Narf	CRISPRko	CTGGCTCAAACCTGAGTGCA	CGG	0.4805	27	15	-	52	9	121250425
+Narf	CRISPRko	ATGATCCAGAAGCTCAAGAA	GGG	0.5989	42	16	+	42	10	121250868
+Narf	CRISPRko	GGTACAGAGATATGGGATGA	TGG	0.4424	31	17	-	56	6	121245380
+Narf	CRISPRko	GATGGGGCCCAGCCAAGCGA	TGG	0.3712	22	18	+	68	2	121238484
+Narf	CRISPRko	CAGGACATGAAGGAAGTCCT	TGG	0.4018	30	19	-	62	3	121242616
+Narf	CRISPRko	TACAGAGATATGGGATGATG	GGG	0.9616	73	20	-	22	6	121245378
+Narf	CRISPRko	CCGGTATCACCAACACAGTG	AGG	0.8352	63	21	+	32	5	121245026
+Narf	CRISPRko	GTACAGAGATATGGGATGAT	GGG	0.503	50	22	-	50	6	121245379
+Narf	CRISPRko	GCACCTCCACAAAGTGGTAT	GGG	0.2071	17	23	-	84	10	121250886
+Narf	CRISPRko	TTGGGTCAGATATGCTGAGC	GGG	0.5314	54	24	+	47	6	121245356
+Narf	CRISPRko	CAGACGGCCTGGATATCAAG	TGG	0.1669	16	25	+	87	11	121252914
+Narf	CRISPRko	CCCTGTGAACCCCTTACCTC	TGG	-0.02632	1	26	-	104	10	121250918
+Narf	CRISPRko	GGTCATGGGCTCCTTGGTGA	AGG	1.164	95	27	+	12	6	121245431
+Narf	CRISPRko	GTTGGGTCAGATATGCTGAG	CGG	1.094	92	28	+	16	6	121245355
+Narf	CRISPRko	AGAGAGCAGTACACATGTGC	AGG	0.8641	82	29	+	30	11	121252804
+Narf	CRISPRko	GTCAGAAGGAGTTTGTACGC	CGG	0.7724	79	30	+	35	5	121245007
+Narf	CRISPRko	GCTCGCGTGTCCCAGAGGTA	AGG	0.2602	35	31	+	80	10	121250918
+Narf	CRISPRko	TGGGGCCCAGCCAAGCGATG	GGG	0.2329	33	32	+	82	2	121238486
+Narf	CRISPRko	CAGCATTCTGGAGAGTCAGA	AGG	0.6249	75	33	+	41	5	121244993
+Narf	CRISPRko	GGTTACCCTTGAGAAGAACG	GGG	1.345	111	34	+	6	10	121250795
+Narf	CRISPRko	GCTGTTTGGCGAGCACGTGG	AGG	1.011	99	35	+	19	9	121250403
+Narf	CRISPRko	ATGTTGACCTCTGCCTGTCC	TGG	0.5582	74	36	+	44	5	121245066
+Narf	CRISPRko	GCAAAATAAGGTAGAGACTG	AGG	1.246	109	37	-	9	4	121244645
+Narf	CRISPRko	CCTGTGAACCCCTTACCTCT	GGG	0.247	40	38	-	81	10	121250917
+Narf	CRISPRko	GAGGTTTGGAGACATGAAGG	AGG	0.9072	96	39	+	26	9	121250307
+Narf	CRISPRko	TGTGGCTTCCTCAAAAGTCT	TGG	0.005216	24	40	+	98	4	121244722
+Narf	CRISPRko	TCTACTCCGGCATCCCTGTG	CGG	0.644	86	41	+	39	11	121252776
+Narf	CRISPRko	GTGTGAGCACTGCACACGAA	AGG	0.2764	47	42	+	78	1	121237347
+Narf	CRISPRko	AAGGAGCCCATGACCTGCTG	GGG	0.9816	107	43	-	21	6	121245412
+Narf	CRISPRko	TACCCTTGAGAAGAACGGGG	AGG	0.9325	105	44	+	24	10	121250798
+Narf	CRISPRko	GACTTGGCGGTACAGAGATA	TGG	-0.2205	6	45	-	124	6	121245388
+Narf	CRISPRko	AGATATGCTGAGCGGGTGCT	GGG	-0.04904	23	46	+	108	6	121245363
+Narf	CRISPRko	ACTTGGCGGTACAGAGATAT	GGG	-0.1861	12	47	-	121	6	121245387
+Narf	CRISPRko	AACTCACTCCATCATGACGC	TGG	0.4177	76	48	-	59	9	121250326
+Narf	CRISPRko	AAGTCCCCCCAGCAGGTCAT	GGG	0.4496	83	49	+	55	6	121245417
+Narf	CRISPRko	ACGCTGCAGCAAAGCGCAGT	AGG	0.1204	51	50	-	92	10	121250811
+Narf	CRISPRko	TGCACATGTGTACTGCTCTC	TGG	-0.4285	8	51	-	136	11	121252791
+Narf	CRISPRko	ACGCGAGCACCTCCACAAAG	TGG	1.155	131	52	-	13	10	121250892
+Narf	CRISPRko	GAGGTTACCCTTGAGAAGAA	CGG	0.3226	72	53	+	74	10	121250793
+Narf	CRISPRko	CAGATATGCTGAGCGGGTGC	TGG	-0.04748	39	54	+	107	6	121245362
+Narf	CRISPRko	TACCTCAAAGCACAGAGTTC	TGG	-0.002797	46	55	+	100	4	121244619
+Narf	CRISPRko	ATGGGGCCCAGCCAAGCGAT	GGG	-0.5079	2	56	+	144	2	121238485
+Narf	CRISPRko	ACATGTGTACTGCTCTCTGG	GGG	1.289	140	57	-	8	11	121252788
+Narf	CRISPRko	GTGGCAGTCCAGCGTCATGA	TGG	0.4813	98	58	+	51	9	121250329
+Narf	CRISPRko	GGAGTTGTACCAGGAGTGGC	TGG	0.1604	61	59	+	89	11	121252825
+Narf	CRISPRko	TCAGGTTGCTTAAATGGCAG	AGG	0.4079	89	60	+	61	11	121252694
+Narf	CRISPRko	TTGAATTTAGCAGCAAAATA	AGG	-0.4803	10	61	-	142	4	121244657
+Narf	CRISPRko	GGAGCCCATGACCTGCTGGG	GGG	1.208	144	62	-	10	6	121245410
+Narf	CRISPRko	GGAGCTGTTTGGCGAGCACG	TGG	0.8007	123	63	+	33	9	121250400
+Narf	CRISPRko	ATATCCAGGCCGTCTGTGCA	GGG	0.05683	62	64	-	95	11	121252896
+Narf	CRISPRko	CAAACTCCCATACCACTTTG	TGG	-0.1807	38	65	+	119	10	121250891
+Narf	CRISPRko	ACTGACTGTGTGCTGACATC	AGG	0.109	64	66	+	93	7	121247051
+Narf	CRISPRko	TCCTGAATCTTAATAAGGTA	TGG	-0.1683	41	67	+	116	3	121242645
+Narf	CRISPRko	TGAATCTCCCAAAGTCCAGG	AGG	0.8556	127	68	+	31	11	121252855
+Narf	CRISPRko	ACCATACCTTATTAAGATTC	AGG	-0.4402	20	69	-	139	3	121242635
+Narf	CRISPRko	TACTGAATCTCCCAAAGTCC	AGG	0.3364	88	70	+	72	11	121252852
+Narf	CRISPRko	CTCAAACCTGAGTGCACGGT	AGG	-0.07042	53	71	-	109	9	121250421
+Narf	CRISPRko	CGTTTTCAGACACGCAGCCA	AGG	-0.2456	37	72	+	125	9	121250379
+Narf	CRISPRko	CCGAGCACCATTTAAAGTAG	TGG	-0.1475	49	73	-	114	7	121247014
+Narf	CRISPRko	CACATGTGTACTGCTCTCTG	GGG	0.7642	129	74	-	36	11	121252789
+Narf	CRISPRko	TCCTTGCTATGATAAGAAGC	TGG	0.3205	90	75	+	75	7	121246981
+Narf	CRISPRko	AGGAGCCCATGACCTGCTGG	GGG	1.035	148	76	-	18	6	121245411
+Narf	CRISPRko	GCTTGGCTGGGCCCCATCAC	TGG	-0.5216	21	77	-	146	2	121238468
+Narf	CRISPRko	ACTACTTTAAATGGTGCTCG	GGG	-0.09679	56	78	+	112	7	121247027
+Narf	CRISPRko	AGCACCTCCACAAAGTGGTA	TGG	-0.07521	58	79	-	111	10	121250887
+Narf	CRISPRko	AGGTGAAATTGCTCAGATAA	TGG	-0.9017	11	80	+	158	8	121249144
+Narf	CRISPRko	TTGTACCAGGAGTGGCTGGA	GGG	0.3322	97	81	+	73	11	121252829
+Narf	CRISPRko	CAGTACCCTCCAGCCACTCC	TGG	0.3454	100	82	-	70	11	121252823
+Narf	CRISPRko	CAGGAGAATGTGTCCAGTGA	TGG	-0.4998	28	83	+	143	2	121238466
+Narf	CRISPRko	TCCAGCTTCTTATCATAGCA	AGG	-0.2808	44	84	-	128	7	121246971
+Narf	CRISPRko	GATGCATCTAGAAGGCTGTG	TGG	1.566	172	85	+	1	4	121244704
+Narf	CRISPRko	GTTGTACCAGGAGTGGCTGG	AGG	0.9504	151	86	+	23	11	121252828
+Narf	CRISPRko	GAAGCTGGAAGCTCTTCGAG	AGG	0.7616	137	87	+	37	7	121246996
+Narf	CRISPRko	GTGCTCACCAGGACAGGCAG	AGG	1.003	154	88	-	20	5	121245062
+Narf	CRISPRko	GGACAGGCAGAGGTCAACAT	GGG	1.177	165	89	-	11	5	121245052
+Narf	CRISPRko	TCACACAGCTGTCACATGCC	AGG	0.5874	133	90	-	43	3	121242557
+Narf	CRISPRko	TTTGTCACCACTTGATATCC	AGG	-0.9517	19	91	-	161	11	121252910
+Narf	CRISPRko	GAAGGGAGAGTTCCATAAAT	TGG	-0.9734	18	92	+	163	3	121242517
+Narf	CRISPRko	TCATGTCCTGAATCTTAATA	AGG	-0.8913	25	93	+	157	3	121242640
+Narf	CRISPRko	GACCTCCCCGTTCTTCTCAA	GGG	0.3886	119	94	-	64	10	121250789
+Narf	CRISPRko	TGACAGCTGTGTGACTGTGG	AGG	1.31	179	95	+	7	3	121242577
+Narf	CRISPRko	ACATGTGCAGGAGTTGTACC	AGG	0.3894	124	96	+	63	11	121252816
+Narf	CRISPRko	GATATCCAGGCCGTCTGTGC	AGG	-0.3484	57	97	-	132	11	121252897
+Narf	CRISPRko	CTGCGCTTTGCTGCAGCGTA	CGG	-0.8556	34	98	+	155	10	121250826
+Narf	CRISPRko	GCTGGGCTGGCCCCATCGCT	TGG	-0.7318	36	99	-	153	2	121238485
+Narf	CRISPRko	GCACACCAGCTACCAGAGCT	TGG	-0.07329	80	100	+	110	11	121252882
+Narf	CRISPRko	CAGAGGCCAAGCACAGACAG	AGG	1.15	176	101	+	14	11	121252711
+Narf	CRISPRko	TGGTGAAGGATTACTTTGCC	AGG	-0.3179	59	102	+	131	6	121245445
+Narf	CRISPRko	AGCATTGTGCTCACCAGGAC	AGG	0.2816	113	103	-	77	5	121245068
+Narf	CRISPRko	CCACTACTTTAAATGGTGCT	CGG	-1.028	29	104	+	164	7	121247025
+Narf	CRISPRko	TTTTTGCATCAGCCAATTTA	TGG	-2.235	14	105	-	179	3	121242518
+Narf	CRISPRko	GGCCAAGCACAGACAGAGGA	CGG	0.5163	146	106	+	48	11	121252715
+Narf	CRISPRko	AGGACAGGCAGAGGTCAACA	TGG	0.7053	159	107	-	38	5	121245053
+Narf	CRISPRko	AAGCTGGAAGCTCTTCGAGA	GGG	0.9213	173	108	+	25	7	121246997
+Narf	CRISPRko	TGCTCTCTGGGGGCCGCACA	GGG	-0.1889	77	109	-	122	11	121252778
+Narf	CRISPRko	AAAATCAAAAACTGATGACC	AGG	-0.134	87	110	+	113	2	121238447
+Narf	CRISPRko	CAAGTCCCCCCAGCAGGTCA	TGG	0.4149	141	111	+	60	6	121245416
+Narf	CRISPRko	GGAGAATGTGTCCAGTGATG	GGG	0.3438	132	112	+	71	2	121238468
+Narf	CRISPRko	GTTTGGAGACATGAAGGAGG	TGG	0.8681	178	113	+	28	9	121250310
+Narf	CRISPRko	CACGCAGCCAAGGAGCTGTT	TGG	-0.02547	106	114	+	103	9	121250389
+Narf	CRISPRko	AGTGAGTTCAGATGGGCATC	TGG	-0.2708	84	115	+	127	9	121250352
+Narf	CRISPRko	CACTACTTTAAATGGTGCTC	GGG	-1.167	43	116	+	168	7	121247026
+Narf	CRISPRko	TGGGGGCCGCACAGGGATGC	CGG	0.04258	115	117	-	97	11	121252771
+Narf	CRISPRko	GCACTGCTGCAGCAAATGGA	AGG	0.4767	161	118	+	53	11	121252751
+Narf	CRISPRko	TGTCCTCTCTGTTTGAGGTT	TGG	-1.165	48	119	+	167	9	121250293
+Narf	CRISPRko	GTGCTCGCCAAACAGCTCCT	TGG	0.1558	126	120	-	90	9	121250385
+Narf	CRISPRko	CACGCTGTTCCTCACTGTGT	TGG	0.1972	130	121	-	86	5	121245024
+Narf	CRISPRko	GAAAATCTTCCATGTTGTCG	TGG	-0.4534	78	122	+	140	7	121246957
+Narf	CRISPRko	GCTGGCCCCATCGCTTGGCT	GGG	-0.7211	67	123	-	152	2	121238480
+Narf	CRISPRko	AGGTTACCCTTGAGAAGAAC	GGG	-0.175	103	124	+	117	10	121250794
+Narf	CRISPRko	GACCTGCTGGGGGGACTTGG	CGG	0.311	145	125	-	76	6	121245401
+Narf	CRISPRko	GGCCGTCCTCTGTCTGTGCT	TGG	-0.6809	71	126	-	151	11	121252706
+Narf	CRISPRko	ATTATCTGAGCAATTTCACC	TGG	-0.4664	81	127	-	141	8	121249131
+Narf	CRISPRko	CCCAGCCAAGGAGAGTGAAG	AGG	0.3861	156	128	+	66	2	121238513
+Narf	CRISPRko	TCTTCTCAAGGGTAACCTCA	TGG	0.457	169	129	-	54	10	121250778
+Narf	CRISPRko	TTTACTCACCAAGACTTTTG	AGG	-1.032	60	130	-	165	4	121244719
+Narf	CRISPRko	CATCACTGGACACATTCTCC	TGG	-0.1949	102	131	-	123	2	121238454
+Narf	CRISPRko	ATGTGACAGCTGTGTGACTG	TGG	0.4184	168	132	+	58	3	121242574
+Narf	CRISPRko	GCACATGTGTACTGCTCTCT	GGG	-0.3985	93	133	-	134	11	121252790
+Narf	CRISPRko	CAAGGAGCCCATGACCTGCT	GGG	0.3715	162	134	-	67	6	121245413
+Narf	CRISPRko	TTCTCTGGAGACAGATTCTG	TGG	0.165	142	135	-	88	7	121246926
+Narf	CRISPRko	AGCTGTGTGACTGTGGAGGA	AGG	0.2627	152	136	+	79	3	121242581

util.py

@@ -0,0 +1,38 @@
+
+import logging
+
+class CustomFormatter(logging.Formatter):
+
+    grey = "\x1b[38;20m"
+    yellow = "\x1b[33;20m"
+    red = "\x1b[31;20m"
+    bold_red = "\x1b[31;1m"
+    reset = "\x1b[0m"
+    format1 = bold_red + "%(asctime)s - %(name)s - %(levelname)s -" + reset
+    format2 = ' %(message)s ' 
+    format3 = bold_red +  "(%(filename)s:%(lineno)d)" + reset
+
+    FORMATS = {
+        logging.DEBUG: format1 + grey + format2 + reset + format3,
+        logging.INFO: format1 + grey + format2 + reset + format3,
+        logging.WARNING: format1 + yellow + format2 + reset + format3,
+        logging.ERROR: format1 + red + format2 + reset + format3,
+        logging.CRITICAL: format1 + bold_red + format2 + reset + format3
+    }
+
+    def format(self, record):
+        log_fmt = self.FORMATS.get(record.levelno)
+        formatter = logging.Formatter(log_fmt)
+        return formatter.format(record)
+
+# create logger
+def get_logger(name):
+    logger = logging.getLogger(name)
+    logger.setLevel(logging.DEBUG)
+    ch = logging.StreamHandler()
+    ch.setLevel(logging.DEBUG)
+    #formatter = logging.Formatter('%(asctime)s - %(name)s - %(levelname)s - %(message)s')
+    ch.setFormatter(CustomFormatter())
+    logger.addHandler(ch)
+    return logger
+