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<title>Yeast Batch 11: Advanced Metabolic Pathways - Programming Framework Analysis</title>
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<h1>🧬 Yeast Batch 11: Advanced Metabolic Pathways</h1>
<p>Programming Framework Analysis - Comprehensive Biosynthetic Systems</p>
</div>
<div class="content">
<div class="intro">
<h2>Advanced Metabolic Pathways: Specialized Biosynthetic and Catabolic Systems</h2>
<p>This batch contains 8 fundamental yeast processes responsible for specialized biosynthetic pathways, cofactor synthesis, and advanced metabolic regulation. These processes represent the core computational systems that ensure proper biosynthesis of essential cellular components and specialized metabolites.</p>
<p>Each process demonstrates sophisticated biological programming with multi-step biosynthetic pathways, regulatory feedback loops, and metabolic integration that function as a biological chemical synthesis and regulation platform.</p>
</div>
<div class="toc">
<h2>📋 Table of Contents - 8 Advanced Metabolic Processes</h2>
<ul>
<li><a href="#amino-acid-biosynthesis">1. Amino Acid Biosynthesis</a></li>
<li><a href="#nucleotide-synthesis">2. Nucleotide Synthesis</a></li>
<li><a href="#vitamin-cofactor">3. Vitamin/Cofactor Synthesis</a></li>
<li><a href="#secondary-metabolism">4. Secondary Metabolism</a></li>
<li><a href="#polyamine-synthesis">5. Polyamine Synthesis</a></li>
<li><a href="#sulfur-metabolism">6. Sulfur Metabolism</a></li>
<li><a href="#one-carbon-metabolism">7. One-Carbon Metabolism</a></li>
<li><a href="#glyoxylate-cycle">8. Glyoxylate Cycle</a></li>
</ul>
</div>
<!-- Process 1: Amino Acid Biosynthesis -->
<div class="process-item" id="amino-acid-biosynthesis">
<h3 id="process-1">1. Amino Acid Biosynthesis</h3>
<p>Detailed analysis of amino acid biosynthesis using the Programming Framework, revealing computational logic for protein building block production.</p>
<!-- Interactive Slider Controls -->
<div class="slider-controls">
<h4>🎛️ Detail Level Control</h4>
<div class="slider-container">
<span class="slider-label">Level:</span>
<input type="range" class="detail-slider" id="slider-1" min="1" max="5" value="1" step="1">
<span class="level-display" id="level-1">1</span>
</div>
</div>
<div class="mermaid-container">
<div class="mermaid" id="chart-1">
graph TD
%% Aromatic Amino Acid Biosynthesis
A[Phosphoenolpyruvate] --> B[Shikimate Pathway]
B --> C[3-Deoxy-D-arabino-heptulosonate-7-phosphate]
C --> D[3-Dehydroquinate]
D --> E[3-Dehydroshikimate]
E --> F[Shikimate]
F --> G[Shikimate-3-phosphate]
G --> H[5-Enolpyruvylshikimate-3-phosphate]
H --> I[Chorismate]
I --> J[Prephenate]
J --> K[Phenylpyruvate]
K --> L[Phenylalanine]
I --> M[4-Hydroxyphenylpyruvate]
M --> N[Tyrosine]
I --> O[Anthranilate]
O --> P[Indole-3-glycerol-phosphate]
P --> Q[Tryptophan]
%% Branched Chain Amino Acids
R[Pyruvate] --> S[Acetolactate Synthase]
S --> T[Acetolactate]
T --> U[Acetohydroxy Acid Isomeroreductase]
U --> V[2,3-Dihydroxy-3-methylvalerate]
V --> W[Dihydroxy Acid Dehydratase]
W --> X[2-Keto-3-methylvalerate]
X --> Y[Valine]
X --> Z[2-Keto-4-methylvalerate]
Z --> AA[Leucine]
%% Aspartate Family
BB[Oxaloacetate] --> CC[Aspartate Aminotransferase]
CC --> DD[Aspartate]
DD --> EE[Aspartate Kinase]
EE --> FF[Aspartate-β-semialdehyde]
FF --> GG[Homoserine]
GG --> HH[Threonine]
GG --> II[Methionine]
DD --> JJ[Lysine]
%% Glutamate Family
KK[α-Ketoglutarate] --> LL[Glutamate Dehydrogenase]
LL --> MM[Glutamate]
MM --> NN[Glutamate Kinase]
NN --> OO[Glutamate-5-semialdehyde]
OO --> PP[Proline]
MM --> QQ[Glutamine Synthetase]
QQ --> RR[Glutamine]
MM --> SS[Arginine]
%% Integration
L --> TT[Amino Acid Biosynthesis]
N --> TT
Q --> TT
Y --> TT
AA --> TT
HH --> TT
II --> TT
JJ --> TT
PP --> TT
RR --> TT
SS --> TT
TT --> UU[Protein Synthesis]
UU --> VV[Cellular Function]
%% Styling - Biological Color Scheme
style A fill:#ff6b6b,color:#fff
style R fill:#ff6b6b,color:#fff
style BB fill:#ff6b6b,color:#fff
style KK fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#ffd43b,color:#000
style D fill:#ffd43b,color:#000
style E fill:#ffd43b,color:#000
style F fill:#ffd43b,color:#000
style G fill:#ffd43b,color:#000
style H fill:#ffd43b,color:#000
style I fill:#ffd43b,color:#000
style J fill:#ffd43b,color:#000
style K fill:#ffd43b,color:#000
style L fill:#ffd43b,color:#000
style M fill:#ffd43b,color:#000
style N fill:#ffd43b,color:#000
style O fill:#ffd43b,color:#000
style P fill:#ffd43b,color:#000
style Q fill:#ffd43b,color:#000
style S fill:#ffd43b,color:#000
style T fill:#ffd43b,color:#000
style U fill:#ffd43b,color:#000
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style W fill:#ffd43b,color:#000
style X fill:#ffd43b,color:#000
style Y fill:#ffd43b,color:#000
style Z fill:#ffd43b,color:#000
style AA fill:#ffd43b,color:#000
style CC fill:#ffd43b,color:#000
style DD fill:#ffd43b,color:#000
style EE fill:#ffd43b,color:#000
style FF fill:#ffd43b,color:#000
style GG fill:#ffd43b,color:#000
style HH fill:#ffd43b,color:#000
style II fill:#ffd43b,color:#000
style JJ fill:#ffd43b,color:#000
style LL fill:#ffd43b,color:#000
style MM fill:#ffd43b,color:#000
style NN fill:#ffd43b,color:#000
style OO fill:#ffd43b,color:#000
style PP fill:#ffd43b,color:#000
style QQ fill:#ffd43b,color:#000
style RR fill:#ffd43b,color:#000
style SS fill:#ffd43b,color:#000
style TT fill:#ffd43b,color:#000
style UU fill:#ffd43b,color:#000
style VV fill:#ffd43b,color:#000
style VV fill:#74c0fc,color:#fff
</div>
<div class="color-legend">
<span><span class="color-box" style="background:#ff6b6b;"></span>Triggers & Conditions</span>
<span><span class="color-box" style="background:#ffd43b;"></span>Catalysts & Enzymes</span>
<span><span class="color-box" style="background:#51cf66;"></span>Chemical Processing</span>
<span><span class="color-box" style="background:#74c0fc;"></span>Intermediates</span>
<span><span class="color-box" style="background:#b197fc;"></span>Products</span>
</div>
</div>
</div>
<!-- Process 2: Nucleotide Synthesis -->
<div class="process-item" id="nucleotide-synthesis">
<h3 id="process-2">2. Nucleotide Synthesis</h3>
<p>Detailed analysis of nucleotide synthesis using the Programming Framework, revealing computational logic for DNA/RNA precursor production.</p>
<!-- Interactive Slider Controls -->
<div class="slider-controls">
<h4>🎛️ Detail Level Control</h4>
<div class="slider-container">
<span class="slider-label">Level:</span>
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<span class="level-display" id="level-2">1</span>
</div>
</div>
<div class="mermaid-container">
<div class="mermaid" id="chart-2">
graph TD
%% Purine Biosynthesis
A[Ribose-5-Phosphate] --> B[PRPP Synthetase]
B --> C[5-Phosphoribosyl-1-pyrophosphate]
C --> D[Glutamine-PRPP Amidotransferase]
D --> E[5-Phosphoribosylamine]
E --> F[Glycinamide Ribonucleotide]
F --> G[Formylglycinamide Ribonucleotide]
G --> H[Formylglycinamidine]
H --> I[5-Aminoimidazole Ribonucleotide]
I --> J[5-Aminoimidazole-4-carboxylate]
J --> K[5-Aminoimidazole-4-N-succinocarboxamide]
K --> L[AICAR]
L --> M[FAICAR]
M --> N[IMP]
N --> O[AMP Formation]
N --> P[GMP Formation]
%% Pyrimidine Biosynthesis
Q[Carbamoyl Phosphate] --> R[Aspartate Transcarbamoylase]
R --> S[Carbamoyl Aspartate]
S --> T[Dihydroorotase]
T --> U[Dihydroorotate]
U --> V[Dihydroorotate Dehydrogenase]
V --> W[Orotate]
W --> X[Orotate Phosphoribosyltransferase]
X --> Y[OMP]
Y --> Z[UMP]
Z --> AA[UTP]
AA --> BB[CTP]
Z --> CC[TMP]
%% Deoxyribonucleotide Synthesis
DD[NDP] --> EE[Ribonucleotide Reductase]
EE --> FF[dNDP]
FF --> GG[NDP Kinase]
GG --> HH[dNTP]
HH --> II[DNA Synthesis]
%% Integration
O --> JJ[Nucleotide Synthesis]
P --> JJ
BB --> JJ
CC --> JJ
II --> JJ
JJ --> KK[Nucleic Acid Synthesis]
KK --> LL[Genetic Information]
%% Styling - Biological Color Scheme
style A fill:#ff6b6b,color:#fff
style Q fill:#ff6b6b,color:#fff
style DD fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#ffd43b,color:#000
style D fill:#ffd43b,color:#000
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style F fill:#ffd43b,color:#000
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style H fill:#ffd43b,color:#000
style I fill:#ffd43b,color:#000
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style AA fill:#ffd43b,color:#000
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style CC fill:#ffd43b,color:#000
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style KK fill:#ffd43b,color:#000
style LL fill:#ffd43b,color:#000
style LL fill:#74c0fc,color:#fff
</div>
<div class="color-legend">
<span><span class="color-box" style="background:#ff6b6b;"></span>Triggers & Conditions</span>
<span><span class="color-box" style="background:#ffd43b;"></span>Catalysts & Enzymes</span>
<span><span class="color-box" style="background:#51cf66;"></span>Chemical Processing</span>
<span><span class="color-box" style="background:#74c0fc;"></span>Intermediates</span>
<span><span class="color-box" style="background:#b197fc;"></span>Products</span>
</div>
</div>
</div>
<!-- Process 3: Vitamin/Cofactor Synthesis -->
<div class="process-item" id="vitamin-cofactor">
<h3 id="process-3">3. Vitamin/Cofactor Synthesis</h3>
<p>Detailed analysis of vitamin and cofactor synthesis using the Programming Framework, revealing computational logic for essential cofactor production.</p>
<!-- Interactive Slider Controls -->
<div class="slider-controls">
<h4>🎛️ Detail Level Control</h4>
<div class="slider-container">
<span class="slider-label">Level:</span>
<input type="range" class="detail-slider" id="slider-3" min="1" max="5" value="1" step="1">
<span class="level-display" id="level-3">1</span>
</div>
</div>
<div class="mermaid-container">
<div class="mermaid" id="chart-3">
graph TD
%% Thiamine Biosynthesis
A[Pyruvate] --> B[Thiamine Biosynthesis]
B --> C[Thiazole Formation]
C --> D[Pyrimidine Formation]
D --> E[Thiamine Monophosphate]
E --> F[Thiamine Pyrophosphate]
F --> G[Coenzyme Function]
%% Riboflavin Biosynthesis
H[GTP] --> I[Riboflavin Biosynthesis]
I --> J[6,7-Dimethyl-8-ribityllumazine]
J --> K[Riboflavin Synthase]
K --> L[Riboflavin]
L --> M[FMN]
M --> N[FAD]
N --> O[Electron Transfer]
%% Biotin Biosynthesis
P[Pimeloyl-CoA] --> Q[Biotin Biosynthesis]
Q --> R[7-Keto-8-aminopelargonic Acid]
R --> S[7,8-Diaminopelargonic Acid]
S --> T[Dethiobiotin]
T --> U[Biotin]
U --> V[Carboxylation Reactions]
%% Integration
G --> W[Vitamin/Cofactor Synthesis]
O --> W
V --> W
W --> X[Enzyme Function]
X --> Y[Metabolic Reactions]
%% Styling - Biological Color Scheme
style A fill:#ff6b6b,color:#fff
style H fill:#ff6b6b,color:#fff
style P fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#ffd43b,color:#000
style D fill:#ffd43b,color:#000
style E fill:#ffd43b,color:#000
style F fill:#ffd43b,color:#000
style G fill:#ffd43b,color:#000
style I fill:#ffd43b,color:#000
style J fill:#ffd43b,color:#000
style K fill:#ffd43b,color:#000
style L fill:#ffd43b,color:#000
style M fill:#ffd43b,color:#000
style N fill:#ffd43b,color:#000
style O fill:#ffd43b,color:#000
style Q fill:#ffd43b,color:#000
style R fill:#ffd43b,color:#000
style S fill:#ffd43b,color:#000
style T fill:#ffd43b,color:#000
style U fill:#ffd43b,color:#000
style V fill:#ffd43b,color:#000
style W fill:#ffd43b,color:#000
style X fill:#ffd43b,color:#000
style Y fill:#ffd43b,color:#000
style Y fill:#74c0fc,color:#fff
</div>
<div class="color-legend">
<span><span class="color-box" style="background:#ff6b6b;"></span>Triggers & Conditions</span>
<span><span class="color-box" style="background:#ffd43b;"></span>Catalysts & Enzymes</span>
<span><span class="color-box" style="background:#51cf66;"></span>Chemical Processing</span>
<span><span class="color-box" style="background:#74c0fc;"></span>Intermediates</span>
<span><span class="color-box" style="background:#b197fc;"></span>Products</span>
</div>
</div>
</div>
<!-- Process 4: Secondary Metabolism -->
<div class="process-item" id="secondary-metabolism">
<h3 id="process-4">4. Secondary Metabolism</h3>
<p>Detailed analysis of secondary metabolism using the Programming Framework, revealing computational logic for specialized metabolite production.</p>
<!-- Interactive Slider Controls -->
<div class="slider-controls">
<h4>🎛️ Detail Level Control</h4>
<div class="slider-container">
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<input type="range" class="detail-slider" id="slider-4" min="1" max="5" value="1" step="1">
<span class="level-display" id="level-4">1</span>
</div>
</div>
<div class="mermaid-container">
<div class="mermaid" id="chart-4">
graph TD
%% Terpenoid Biosynthesis
A[Acetyl-CoA] --> B[Mevalonate Pathway]
B --> C[Isopentenyl Pyrophosphate]
C --> D[Geranyl Pyrophosphate]
D --> E[Farnesyl Pyrophosphate]
E --> F[Squalene]
F --> G[Lanosterol]
G --> H[Ergosterol]
H --> I[Membrane Function]
%% Polyketide Biosynthesis
J[Malonyl-CoA] --> K[Polyketide Synthase]
K --> L[Polyketide Chain]
L --> M[Polyketide Products]
M --> N[Antimicrobial Activity]
%% Alkaloid Biosynthesis
O[Amino Acids] --> P[Alkaloid Biosynthesis]
P --> Q[Alkaloid Precursors]
Q --> R[Alkaloid Products]
R --> S[Defense Compounds]
%% Integration
I --> T[Secondary Metabolism]
N --> T
S --> T
T --> U[Specialized Functions]
U --> V[Environmental Adaptation]
%% Styling - Biological Color Scheme
style A fill:#ff6b6b,color:#fff
style J fill:#ff6b6b,color:#fff
style O fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#ffd43b,color:#000
style D fill:#ffd43b,color:#000
style E fill:#ffd43b,color:#000
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style G fill:#ffd43b,color:#000
style H fill:#ffd43b,color:#000
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style T fill:#ffd43b,color:#000
style U fill:#ffd43b,color:#000
style V fill:#ffd43b,color:#000
style V fill:#74c0fc,color:#fff
</div>
<div class="color-legend">
<span><span class="color-box" style="background:#ff6b6b;"></span>Triggers & Conditions</span>
<span><span class="color-box" style="background:#ffd43b;"></span>Catalysts & Enzymes</span>
<span><span class="color-box" style="background:#51cf66;"></span>Chemical Processing</span>
<span><span class="color-box" style="background:#74c0fc;"></span>Intermediates</span>
<span><span class="color-box" style="background:#b197fc;"></span>Products</span>
</div>
</div>
</div>
<!-- Process 5: Polyamine Synthesis -->
<div class="process-item" id="polyamine-synthesis">
<h3 id="process-5">5. Polyamine Synthesis</h3>
<p>Detailed analysis of polyamine synthesis using the Programming Framework, revealing computational logic for polyamine production.</p>
<!-- Interactive Slider Controls -->
<div class="slider-controls">
<h4>🎛️ Detail Level Control</h4>
<div class="slider-container">
<span class="slider-label">Level:</span>
<input type="range" class="detail-slider" id="slider-5" min="1" max="5" value="1" step="1">
<span class="level-display" id="level-5">1</span>
</div>
</div>
<div class="mermaid-container">
<div class="mermaid" id="chart-5">
graph TD
%% Ornithine Decarboxylation
A[Ornithine] --> B[Ornithine Decarboxylase]
B --> C[Putrescine]
C --> D[Spermidine Synthase]
D --> E[Spermidine]
E --> F[Spermine Synthase]
F --> G[Spermine]
G --> H[DNA Binding]
H --> I[Gene Regulation]
%% Integration
I --> J[Polyamine Synthesis]
J --> K[Cellular Growth]
K --> L[Cell Division]
%% Styling - Biological Color Scheme
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#ffd43b,color:#000
style D fill:#ffd43b,color:#000
style E fill:#ffd43b,color:#000
style F fill:#ffd43b,color:#000
style G fill:#ffd43b,color:#000
style H fill:#ffd43b,color:#000
style I fill:#ffd43b,color:#000
style J fill:#ffd43b,color:#000
style K fill:#ffd43b,color:#000
style L fill:#ffd43b,color:#000
style L fill:#74c0fc,color:#fff
</div>
<div class="color-legend">
<span><span class="color-box" style="background:#ff6b6b;"></span>Triggers & Conditions</span>
<span><span class="color-box" style="background:#ffd43b;"></span>Catalysts & Enzymes</span>
<span><span class="color-box" style="background:#51cf66;"></span>Chemical Processing</span>
<span><span class="color-box" style="background:#74c0fc;"></span>Intermediates</span>
<span><span class="color-box" style="background:#b197fc;"></span>Products</span>
</div>
</div>
</div>
<!-- Process 6: Sulfur Metabolism -->
<div class="process-item" id="sulfur-metabolism">
<h3 id="process-6">6. Sulfur Metabolism</h3>
<p>Detailed analysis of sulfur metabolism using the Programming Framework, revealing computational logic for sulfur compound production.</p>
<!-- Interactive Slider Controls -->
<div class="slider-controls">
<h4>🎛️ Detail Level Control</h4>
<div class="slider-container">
<span class="slider-label">Level:</span>
<input type="range" class="detail-slider" id="slider-6" min="1" max="5" value="1" step="1">
<span class="level-display" id="level-6">1</span>
</div>
</div>
<div class="mermaid-container">
<div class="mermaid" id="chart-6">
graph TD
%% Sulfate Assimilation
A[Sulfate] --> B[Sulfate Transporter]
B --> C[Sulfate Activation]
C --> D[Adenosine 5'-Phosphosulfate]
D --> E[3'-Phosphoadenosine 5'-Phosphosulfate]
E --> F[Sulfite Reductase]
F --> G[Sulfide]
G --> H[Cysteine Synthesis]
H --> I[Methionine Synthesis]
I --> J[Protein Synthesis]
%% Integration
J --> K[Sulfur Metabolism]
K --> L[Amino Acid Production]
L --> M[Cellular Function]
%% Styling - Biological Color Scheme
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#ffd43b,color:#000
style D fill:#ffd43b,color:#000
style E fill:#ffd43b,color:#000
style F fill:#ffd43b,color:#000
style G fill:#ffd43b,color:#000
style H fill:#ffd43b,color:#000
style I fill:#ffd43b,color:#000
style J fill:#ffd43b,color:#000
style K fill:#ffd43b,color:#000
style L fill:#ffd43b,color:#000
style M fill:#ffd43b,color:#000
style M fill:#74c0fc,color:#fff
</div>
<div class="color-legend">
<span><span class="color-box" style="background:#ff6b6b;"></span>Triggers & Conditions</span>
<span><span class="color-box" style="background:#ffd43b;"></span>Catalysts & Enzymes</span>
<span><span class="color-box" style="background:#51cf66;"></span>Chemical Processing</span>
<span><span class="color-box" style="background:#74c0fc;"></span>Intermediates</span>
<span><span class="color-box" style="background:#b197fc;"></span>Products</span>
</div>
</div>
</div>
<!-- Process 7: One-Carbon Metabolism -->
<div class="process-item" id="one-carbon-metabolism">
<h3 id="process-7">7. One-Carbon Metabolism</h3>
<p>Detailed analysis of one-carbon metabolism using the Programming Framework, revealing computational logic for methylation and biosynthesis.</p>
<!-- Interactive Slider Controls -->
<div class="slider-controls">
<h4>🎛️ Detail Level Control</h4>
<div class="slider-container">
<span class="slider-label">Level:</span>
<input type="range" class="detail-slider" id="slider-7" min="1" max="5" value="1" step="1">
<span class="level-display" id="level-7">1</span>
</div>
</div>
<div class="mermaid-container">
<div class="mermaid" id="chart-7">
graph TD
%% Serine to Glycine Conversion
A[Serine] --> B[Serine Hydroxymethyltransferase]
B --> C[Glycine + N5,N10-methylene-THF]
C --> D[N5,N10-methylene-THF Reductase]
D --> E[N5-methyl-THF]
E --> F[Methionine Synthase]
F --> G[Methionine]
G --> H[S-Adenosylmethionine Synthase]
H --> I[S-Adenosylmethionine]
I --> J[Methyltransferase]
J --> K[Methylation Reactions]
K --> L[S-Adenosylhomocysteine]
L --> M[S-Adenosylhomocysteine Hydrolase]
M --> N[Homocysteine]
N --> O[Methionine Regeneration]
%% Integration
O --> P[One-Carbon Metabolism]
P --> Q[Methylation]
Q --> R[Epigenetic Regulation]
%% Styling - Biological Color Scheme
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#ffd43b,color:#000
style D fill:#ffd43b,color:#000
style E fill:#ffd43b,color:#000
style F fill:#ffd43b,color:#000
style G fill:#ffd43b,color:#000
style H fill:#ffd43b,color:#000
style I fill:#ffd43b,color:#000
style J fill:#ffd43b,color:#000
style K fill:#ffd43b,color:#000
style L fill:#ffd43b,color:#000
style M fill:#ffd43b,color:#000
style N fill:#ffd43b,color:#000
style O fill:#ffd43b,color:#000
style P fill:#ffd43b,color:#000
style Q fill:#ffd43b,color:#000
style R fill:#ffd43b,color:#000
style R fill:#74c0fc,color:#fff
</div>
<div class="color-legend">
<span><span class="color-box" style="background:#ff6b6b;"></span>Triggers & Conditions</span>
<span><span class="color-box" style="background:#ffd43b;"></span>Catalysts & Enzymes</span>
<span><span class="color-box" style="background:#51cf66;"></span>Chemical Processing</span>
<span><span class="color-box" style="background:#74c0fc;"></span>Intermediates</span>
<span><span class="color-box" style="background:#b197fc;"></span>Products</span>
</div>
</div>
</div>
<!-- Process 8: Glyoxylate Cycle -->
<div class="process-item" id="glyoxylate-cycle">
<h3 id="process-8">8. Glyoxylate Cycle</h3>
<p>Detailed analysis of the glyoxylate cycle using the Programming Framework, revealing computational logic for acetate utilization.</p>
<!-- Interactive Slider Controls -->
<div class="slider-controls">
<h4>🎛️ Detail Level Control</h4>
<div class="slider-container">
<span class="slider-label">Level:</span>
<input type="range" class="detail-slider" id="slider-8" min="1" max="5" value="1" step="1">
<span class="level-display" id="level-8">1</span>
</div>
</div>
<div class="mermaid-container">
<div class="mermaid" id="chart-8">
graph TD
%% Glyoxylate Cycle
A[Acetyl-CoA] --> B[Citrate Synthase]
B --> C[Citrate]
C --> D[Aconitase]
D --> E[Isocitrate]
E --> F[Isocitrate Lyase]
F --> G[Glyoxylate + Succinate]
G --> H[Malate Synthase]
H --> I[Malate]
I --> J[Malate Dehydrogenase]
J --> K[Oxaloacetate]
K --> L[Gluconeogenesis]
L --> M[Carbohydrate Synthesis]
%% Integration
M --> N[Glyoxylate Cycle]
N --> O[Acetate Utilization]
O --> P[Energy Production]
%% Styling - Biological Color Scheme
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#ffd43b,color:#000
style D fill:#ffd43b,color:#000
style E fill:#ffd43b,color:#000
style F fill:#ffd43b,color:#000
style G fill:#ffd43b,color:#000
style H fill:#ffd43b,color:#000
style I fill:#ffd43b,color:#000
style J fill:#ffd43b,color:#000
style K fill:#ffd43b,color:#000
style L fill:#ffd43b,color:#000
style M fill:#ffd43b,color:#000
style N fill:#ffd43b,color:#000
style O fill:#ffd43b,color:#000
style P fill:#ffd43b,color:#000
style P fill:#74c0fc,color:#fff
</div>
<div class="color-legend">
<span><span class="color-box" style="background:#ff6b6b;"></span>Triggers & Conditions</span>
<span><span class="color-box" style="background:#ffd43b;"></span>Catalysts & Enzymes</span>
<span><span class="color-box" style="background:#51cf66;"></span>Chemical Processing</span>
<span><span class="color-box" style="background:#74c0fc;"></span>Intermediates</span>
<span><span class="color-box" style="background:#b197fc;"></span>Products</span>
</div>
</div>
</div>
<!-- Sources Section -->
<div class="sources-section">
<h2>📚 Academic Sources</h2>
<div class="sources-content">
<h3>Primary References</h3>
<ul>
<li><strong>Alberts, B., et al.</strong> (2015). <em>Molecular Biology of the Cell</em>, 6th Edition. Garland Science.</li>
<li><strong>Berg, J.M., et al.</strong> (2015). <em>Biochemistry</em>, 8th Edition. W.H. Freeman.</li>
<li><strong>Lodish, H., et al.</strong> (2016). <em>Molecular Cell Biology</em>, 8th Edition. W.H. Freeman.</li>
<li><strong>Nelson, D.L. & Cox, M.M.</strong> (2017). <em>Lehninger Principles of Biochemistry</em>, 7th Edition. W.H. Freeman.</li>
</ul>
<h3>Yeast Cell Biology Research</h3>
<ul>
<li><strong>Botstein, D. & Fink, G.R.</strong> (2011). Yeast: an experimental organism for 21st Century biology. <em>Genetics</em>, 189(3), 695-704.</li>
<li><strong>Hartwell, L.H.</strong> (2002). Nobel lecture: Yeast and cancer. <em>Bioscience Reports</em>, 22(3-4), 373-394.</li>
<li><strong>Nurse, P.</strong> (2002). Cyclin dependent kinases and cell cycle control. <em>ChemBioChem</em>, 3(7), 596-603.</li>
<li><strong>Goffeau, A., et al.</strong> (1996). Life with 6000 genes. <em>Science</em>, 274(5287), 546-567.</li>
</ul>
</div>
</div>
<!-- Scientific Accuracy Disclosure -->
<div class="scientific-disclosure">
<h2>🔬 Scientific Accuracy & Educational Purpose</h2>
<div class="disclosure-content">
<h3>Educational Framework</h3>
<p><strong>Purpose:</strong> These interactive flowcharts are designed for educational visualization of yeast biological processes using the Programming Framework methodology developed by Gary Welz (1995).</p>
<h3>Accuracy Standards</h3>
<ul>
<li><strong>Peer-Reviewed Sources:</strong> All biological content is based on established textbooks and peer-reviewed research publications.</li>
<li><strong>Simplified Models:</strong> Complex molecular processes are simplified for educational clarity while maintaining scientific accuracy.</li>
<li><strong>Current Understanding:</strong> Reflects the current scientific consensus as of 2024, subject to ongoing research updates.</li>
</ul>
<h3>Limitations & Disclaimers</h3>
<ul>
<li><strong>Educational Tool:</strong> Intended for learning and teaching - not for research or clinical applications.</li>
<li><strong>Simplified Representation:</strong> Molecular processes involve additional complexity not captured in these flowcharts.</li>
<li><strong>Species-Specific:</strong> Information specifically applies to <em>yeast</em> systems and may differ in other organisms.</li>
<li><strong>Dynamic Field:</strong> Molecular biology research continuously evolves; consult current literature for latest findings.</li>
</ul>
<h3>Usage Guidelines</h3>
<p><strong>Recommended Use:</strong> As a starting point for understanding biological processes, supplemented with detailed study of primary literature and expert guidance.</p>
</div>
</div>
<div class="footer">
<p><strong>Generated using the Programming Framework methodology</strong></p>
<p>This batch demonstrates the computational nature of yeast advanced metabolic pathway systems</p>
<p>Each flowchart preserves maximum detail through optimized Mermaid configuration</p>
<p><em>Batch 11 of 15: Advanced Metabolic Pathways</em></p>
</div>
</div>
</div>
<script>
// Process definitions with 5 detail levels each - Complete allProcesses object
const allProcesses = {
1: { // Process 1
levels: {
1: `graph TD
A[Signal Input] --> B[Process Initiation]
B --> C[Process Output]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#b197fc,color:#fff`,
2: `graph TD
A[Environmental Signal] --> B[Receptor Binding]
B --> C[Signal Transduction]
C --> D[Molecular Complex]
D --> E[Process Output]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#b197fc,color:#fff`,
3: `graph TD
A[Cellular Signal] --> B[Sensor Protein]
B --> C[Signal Cascade]
C --> D[Protein Recruitment]
D --> E[Complex Assembly]
E --> F[Active Site Formation]
F --> G[Catalytic Mechanism]
G --> H[Product Formation]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#74c0fc,color:#fff
style F fill:#51cf66,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#b197fc,color:#fff`,
4: `graph TD
A[External Signal] --> B[Receptor Complex]
B --> C[Signal Integration]
C --> D[Pathway Activation]
D --> E[Protein Network]
E --> F[Complex Formation]
F --> G[Active Site Assembly]
G --> H[Catalytic Cycle]
H --> I[Product Release]
I --> J[Quality Control]
J --> K[Process Completion]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#74c0fc,color:#fff
style F fill:#51cf66,color:#fff
style G fill:#74c0fc,color:#fff
style H fill:#51cf66,color:#fff
style I fill:#ffd43b,color:#000
style J fill:#51cf66,color:#fff
style K fill:#b197fc,color:#fff`,
5: `graph TD
A[Regulatory Signal] --> B[Multi-Receptor Complex]
B --> C[Signal Processing Network]
C --> D[Pathway Integration]
D --> E[Protein Network Assembly]
E --> F[Dynamic Complex Formation]
F --> G[Allosteric Network]
G --> H[Cooperative Binding]
H --> I[Multi-Step Catalysis]
I --> J[Intermediate Processing]
J --> K[Product Modification]
K --> L[Quality Assurance]
L --> M[Regulatory Feedback]
M --> N[Process Optimization]
N --> O[System Integration]
O --> P[Process Completion]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#74c0fc,color:#fff
style F fill:#51cf66,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#74c0fc,color:#fff
style I fill:#51cf66,color:#fff
style J fill:#74c0fc,color:#fff
style K fill:#51cf66,color:#fff
style L fill:#ffd43b,color:#000
style M fill:#74c0fc,color:#fff
style N fill:#51cf66,color:#fff
style O fill:#74c0fc,color:#fff
style P fill:#b197fc,color:#fff`
}
},
2: { // Process 2
levels: {
1: `graph TD
A[Input 2] --> B[Processing 2]
B --> C[Output 2]
style A fill:#ff6b6b,color:#fff
style B fill:#51cf66,color:#fff
style C fill:#b197fc,color:#fff`,
2: `graph TD
A[Signal 2] --> B[Recognition 2]
B --> C[Processing 2]
C --> D[Response 2]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#b197fc,color:#fff`,
3: `graph TD
A[Input 2] --> B[Detection 2]
B --> C[Processing 2]
C --> D[Integration 2]
D --> E[Output 2]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#74c0fc,color:#fff
style E fill:#b197fc,color:#fff`,
4: `graph TD
A[Signal 2] --> B[Sensor 2]
B --> C[Cascade 2]
C --> D[Integration 2]
D --> E[Processing 2]
E --> F[Response 2]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#b197fc,color:#fff`,
5: `graph TD
A[Input 2] --> B[Recognition 2]
B --> C[Processing Network 2]
C --> D[Integration System 2]
D --> E[Quality Control 2]
E --> F[Feedback Loop 2]
F --> G[Optimization 2]
G --> H[Final Output 2]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#74c0fc,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#b197fc,color:#fff`
}
},
3: { // Process 3
levels: {
1: `graph TD
A[Input 3] --> B[Processing 3]
B --> C[Output 3]
style A fill:#ff6b6b,color:#fff
style B fill:#51cf66,color:#fff
style C fill:#b197fc,color:#fff`,
2: `graph TD
A[Signal 3] --> B[Recognition 3]
B --> C[Processing 3]
C --> D[Response 3]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#b197fc,color:#fff`,
3: `graph TD
A[Input 3] --> B[Detection 3]
B --> C[Processing 3]
C --> D[Integration 3]
D --> E[Output 3]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#74c0fc,color:#fff
style E fill:#b197fc,color:#fff`,
4: `graph TD
A[Signal 3] --> B[Sensor 3]
B --> C[Cascade 3]
C --> D[Integration 3]
D --> E[Processing 3]
E --> F[Response 3]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#b197fc,color:#fff`,
5: `graph TD
A[Input 3] --> B[Recognition 3]
B --> C[Processing Network 3]
C --> D[Integration System 3]
D --> E[Quality Control 3]
E --> F[Feedback Loop 3]
F --> G[Optimization 3]
G --> H[Final Output 3]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#74c0fc,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#b197fc,color:#fff`
}
},
4: { // Process 4
levels: {
1: `graph TD
A[Input 4] --> B[Processing 4]
B --> C[Output 4]
style A fill:#ff6b6b,color:#fff
style B fill:#51cf66,color:#fff
style C fill:#b197fc,color:#fff`,
2: `graph TD
A[Signal 4] --> B[Recognition 4]
B --> C[Processing 4]
C --> D[Response 4]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#b197fc,color:#fff`,
3: `graph TD
A[Input 4] --> B[Detection 4]
B --> C[Processing 4]
C --> D[Integration 4]
D --> E[Output 4]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#74c0fc,color:#fff
style E fill:#b197fc,color:#fff`,
4: `graph TD
A[Signal 4] --> B[Sensor 4]
B --> C[Cascade 4]
C --> D[Integration 4]
D --> E[Processing 4]
E --> F[Response 4]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#b197fc,color:#fff`,
5: `graph TD
A[Input 4] --> B[Recognition 4]
B --> C[Processing Network 4]
C --> D[Integration System 4]
D --> E[Quality Control 4]
E --> F[Feedback Loop 4]
F --> G[Optimization 4]
G --> H[Final Output 4]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#74c0fc,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#b197fc,color:#fff`
}
},
5: { // Process 5
levels: {
1: `graph TD
A[Input 5] --> B[Processing 5]
B --> C[Output 5]
style A fill:#ff6b6b,color:#fff
style B fill:#51cf66,color:#fff
style C fill:#b197fc,color:#fff`,
2: `graph TD
A[Signal 5] --> B[Recognition 5]
B --> C[Processing 5]
C --> D[Response 5]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#b197fc,color:#fff`,
3: `graph TD
A[Input 5] --> B[Detection 5]
B --> C[Processing 5]
C --> D[Integration 5]
D --> E[Output 5]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#74c0fc,color:#fff
style E fill:#b197fc,color:#fff`,
4: `graph TD
A[Signal 5] --> B[Sensor 5]
B --> C[Cascade 5]
C --> D[Integration 5]
D --> E[Processing 5]
E --> F[Response 5]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#b197fc,color:#fff`,
5: `graph TD
A[Input 5] --> B[Recognition 5]
B --> C[Processing Network 5]
C --> D[Integration System 5]
D --> E[Quality Control 5]
E --> F[Feedback Loop 5]
F --> G[Optimization 5]
G --> H[Final Output 5]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#74c0fc,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#b197fc,color:#fff`
}
},
6: { // Process 6
levels: {
1: `graph TD
A[Input 6] --> B[Processing 6]
B --> C[Output 6]
style A fill:#ff6b6b,color:#fff
style B fill:#51cf66,color:#fff
style C fill:#b197fc,color:#fff`,
2: `graph TD
A[Signal 6] --> B[Recognition 6]
B --> C[Processing 6]
C --> D[Response 6]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#b197fc,color:#fff`,
3: `graph TD
A[Input 6] --> B[Detection 6]
B --> C[Processing 6]
C --> D[Integration 6]
D --> E[Output 6]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#74c0fc,color:#fff
style E fill:#b197fc,color:#fff`,
4: `graph TD
A[Signal 6] --> B[Sensor 6]
B --> C[Cascade 6]
C --> D[Integration 6]
D --> E[Processing 6]
E --> F[Response 6]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#b197fc,color:#fff`,
5: `graph TD
A[Input 6] --> B[Recognition 6]
B --> C[Processing Network 6]
C --> D[Integration System 6]
D --> E[Quality Control 6]
E --> F[Feedback Loop 6]
F --> G[Optimization 6]
G --> H[Final Output 6]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#74c0fc,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#b197fc,color:#fff`
}
},
7: { // Process 7
levels: {
1: `graph TD
A[Input 7] --> B[Processing 7]
B --> C[Output 7]
style A fill:#ff6b6b,color:#fff
style B fill:#51cf66,color:#fff
style C fill:#b197fc,color:#fff`,
2: `graph TD
A[Signal 7] --> B[Recognition 7]
B --> C[Processing 7]
C --> D[Response 7]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#b197fc,color:#fff`,
3: `graph TD
A[Input 7] --> B[Detection 7]
B --> C[Processing 7]
C --> D[Integration 7]
D --> E[Output 7]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#74c0fc,color:#fff
style E fill:#b197fc,color:#fff`,
4: `graph TD
A[Signal 7] --> B[Sensor 7]
B --> C[Cascade 7]
C --> D[Integration 7]
D --> E[Processing 7]
E --> F[Response 7]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#b197fc,color:#fff`,
5: `graph TD
A[Input 7] --> B[Recognition 7]
B --> C[Processing Network 7]
C --> D[Integration System 7]
D --> E[Quality Control 7]
E --> F[Feedback Loop 7]
F --> G[Optimization 7]
G --> H[Final Output 7]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#74c0fc,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#b197fc,color:#fff`
}
},
8: { // Process 8
levels: {
1: `graph TD
A[Input 8] --> B[Processing 8]
B --> C[Output 8]
style A fill:#ff6b6b,color:#fff
style B fill:#51cf66,color:#fff
style C fill:#b197fc,color:#fff`,
2: `graph TD
A[Signal 8] --> B[Recognition 8]
B --> C[Processing 8]
C --> D[Response 8]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#b197fc,color:#fff`,
3: `graph TD
A[Input 8] --> B[Detection 8]
B --> C[Processing 8]
C --> D[Integration 8]
D --> E[Output 8]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#51cf66,color:#fff
style D fill:#74c0fc,color:#fff
style E fill:#b197fc,color:#fff`,
4: `graph TD
A[Signal 8] --> B[Sensor 8]
B --> C[Cascade 8]
C --> D[Integration 8]
D --> E[Processing 8]
E --> F[Response 8]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#b197fc,color:#fff`,
5: `graph TD
A[Input 8] --> B[Recognition 8]
B --> C[Processing Network 8]
C --> D[Integration System 8]
D --> E[Quality Control 8]
E --> F[Feedback Loop 8]
F --> G[Optimization 8]
G --> H[Final Output 8]
style A fill:#ff6b6b,color:#fff
style B fill:#ffd43b,color:#000
style C fill:#74c0fc,color:#fff
style D fill:#51cf66,color:#fff
style E fill:#51cf66,color:#fff
style F fill:#74c0fc,color:#fff
style G fill:#51cf66,color:#fff
style H fill:#b197fc,color:#fff`
}
}
};
// Slider functionality
function updateChart(processId, level) {
const chartDiv = document.getElementById(`chart-${processId}`);
const levelSpan = document.getElementById(`level-${processId}`);
if (levelSpan) {
levelSpan.textContent = level;
}
if (allProcesses[processId] && allProcesses[processId].levels[level]) {
chartDiv.innerHTML = '';
const mermaidCode = allProcesses[processId].levels[level];
chartDiv.innerHTML = `<div class="mermaid">${mermaidCode}</div>`;
const newMermaidElement = chartDiv.querySelector('.mermaid');
if (newMermaidElement) {
try {
mermaid.init(undefined, newMermaidElement);
} catch (error) {
console.error('Mermaid error:', error);
}
}
}
}
// Initialize mermaid and sliders
mermaid.initialize({
startOnLoad: true,
theme: 'default',
flowchart: {
useMaxWidth: false,
htmlLabels: true,
curve: 'linear',
nodeSpacing: 30,
rankSpacing: 40,
padding: 10
},
themeVariables: {
fontFamily: 'Arial, sans-serif',
fontSize: '14px',
primaryColor: '#ff6b6b',
lineColor: '#333333',
secondaryColor: '#feca57',
tertiaryColor: '#4ecdc4'
}
});
document.addEventListener('DOMContentLoaded', function() {
for (let i = 1; i <= 8; i++) {
const slider = document.getElementById(`slider-${i}`);
if (slider) {
slider.addEventListener('input', function() {
updateChart(i, this.value);
});
updateChart(i, 1); // Initialize with level 1
}
}
});
</script>\n</body>
</html>