app.py

import gradio as gr
import numpy as np
import pandas as pd
import plotly.graph_objects as go
from fcsparser import parse
from matplotlib.path import Path

def parse_fcs(file):
    _, data = parse(file.name, reformat_meta=True)
    return data

def update_params(data):
    if data is None: 
        return [gr.Dropdown.update(choices=[])] * 2
    return [gr.Dropdown.update(choices=data.columns.tolist())] * 2

def create_plot(data, x_param, y_param):
    if data is None or not x_param or not y_param:
        return None
    
    fig = go.Figure(data=go.Scattergl(
        x=data[x_param],
        y=data[y_param],
        mode='markers',
        marker=dict(size=2, opacity=0.5)
    ))
    
    fig.update_layout(
        title=f"{x_param} vs {y_param}",
        dragmode='drawclosedpath',
        width=800,
        height=600
    )
    
    return fig

def apply_gate(data, x_param, y_param, fig):
    if data is None or fig is None:
        return "Önce veri yükleyin ve grafik oluşturun", None
    
    # Plotly şekil verisini çıkar
    shapes = fig.get('layout', {}).get('shapes', [])
    if not shapes:
        return "Lütfen önce polygon çizin", None
    
    # Son çizilen şekli al
    last_shape = shapes[-1]
    path = last_shape.get('path', '')
    
    # Path verisini işle (M=move, L=line, Z=close)
    vertices = []
    for part in path.split(' '):
        if part.startswith('M') or part.startswith('L'):
            x, y = part[1:].split(',')
            vertices.append((float(x), float(y)))
    
    if len(vertices) < 3:
        return "Geçerli bir polygon çizilmedi", None
    
    # Matplotlib Path oluştur
    polygon = Path(vertices)
    points = np.vstack([data[x_param], data[y_param]]).T
    
    # İçerde kalan noktaları bul
    mask = polygon.contains_points(points)
    gated_data = data[mask]
    
    # Sonuçları hazırla
    stats = f"""
    Toplam Hücre: {len(data):,}
    Geçitlenen Hücre: {len(gated_data):,} (%{len(gated_data)/len(data)*100:.1f})
    İlk 10 Hücre İndeksi: {gated_data.index[:10].tolist()}
    """
    
    return stats, gated_data

with gr.Blocks(title="FCS Analiz Uygulaması") as demo:
    gr.Markdown("## FCS Dosya Analizi ve Hücre Geçitleme")
    
    with gr.Row():
        with gr.Column():
            file_input = gr.File(label="FCS Dosyası Yükle", type="file")
            x_param = gr.Dropdown(label="X Parametresi")
            y_param = gr.Dropdown(label="Y Parametresi")
            plot_btn = gr.Button("Grafik Oluştur")
            
        with gr.Column():
            plot_output = gr.Plot(label="Dağılım Grafiği")
            gate_btn = gr.Button("Geçitlemeyi Uygula")
            result_output = gr.Textbox(label="Sonuçlar")
            data_output = gr.DataFrame(label="Geçitlenen Veriler", visible=False)

    # State management
    data_state = gr.State()
    fig_state = gr.State()

    # Event handlers
    file_input.change(
        fn=parse_fcs,
        inputs=file_input,
        outputs=data_state
    ).then(
        fn=update_params,
        inputs=data_state,
        outputs=[x_param, y_param]
    )

    plot_btn.click(
        fn=create_plot,
        inputs=[data_state, x_param, y_param],
        outputs=[plot_output]
    ).then(
        lambda fig: fig,
        inputs=plot_output,
        outputs=fig_state
    )

    gate_btn.click(
        fn=apply_gate,
        inputs=[data_state, x_param, y_param, fig_state],
        outputs=[result_output, data_output]
    )

if __name__ == "__main__":
    demo.launch()