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import json
import os
import cv2
import gradio as gr
import numpy as np
import matplotlib
matplotlib.use('Agg') # CRITICAL: Makes matplotlib thread-safe for Hugging Face web servers
import matplotlib.pyplot as plt
import torch
import torch.nn.functional as F
import spaces
from PIL import Image
from pycocotools import mask as mask_utils
from transformers import Mask2FormerImageProcessor, Mask2FormerForUniversalSegmentation
# ==========================================
# 1. MODEL INITIALIZATION & GLOBAL CONFIG
# ==========================================
MODEL_ID = "facebook/mask2former-swin-tiny-coco-instance"
# Load processor globally
processor = Mask2FormerImageProcessor.from_pretrained(MODEL_ID)
# Attempt to load local trained model checkpoints with fallbacks
model = None
checkpoint_paths = ["./mask2former2", "./mask2former-manual-save", MODEL_ID]
for path in checkpoint_paths:
try:
model = Mask2FormerForUniversalSegmentation.from_pretrained(
path,
ignore_mismatched_sizes=True
)
print(f"Successfully loaded model checkpoint from: '{path}'")
break
except Exception as e:
print(f"Info: Could not load model from '{path}'. Reason: {e}")
if model is None:
raise RuntimeError("Failed to load any valid Mask2Former model checkpoint.")
# Model output integer class mapping (0-indexed)
ID_TO_LABEL = {
0: "Conidial Head",
1: "Hyphae",
2: "Free Spore",
3: "Spore Clump",
4: "Debris",
5: "Fiber",
6: "Bubble",
}
SHOW_CATEGORIES = {label: True for label in ID_TO_LABEL.values()}
# Distinct color palette per class [R, G, B] normalized to 0.0-1.0
CLASS_COLORS = [
[1.0, 0.0, 0.0], # 0: Conidial Head -> Red
[0.0, 1.0, 0.0], # 1: Hyphae -> Green
[0.0, 0.0, 1.0], # 2: Free Spore -> Blue
[1.0, 1.0, 0.0], # 3: Spore Clump -> Yellow
[1.0, 0.0, 1.0], # 4: Debris -> Magenta
[0.0, 1.0, 1.0], # 5: Fiber -> Cyan
[0.5, 0.5, 0.5], # 6: Bubble -> Gray
]
# Sliding Window Hyperparameters
WINDOW_SIZE = 512
STRIDE = 384
# ==========================================
# 2. HELPER UTILITIES
# ==========================================
def binary_mask_to_rle(binary_mask: np.ndarray) -> dict:
"""Converts a 2D boolean NumPy mask [H, W] into standard COCO RLE format."""
fortran_mask = np.asfortranarray(binary_mask.astype(np.uint8))
rle = mask_utils.encode(fortran_mask)
rle["counts"] = rle["counts"].decode("utf-8") # Decode bytes to UTF-8 for JSON
return rle
# ==========================================
# 3. INFERENCE ENGINE (WITH PROBABILITY ACCUMULATION & RLE)
# ==========================================
@spaces.GPU(duration=30)
def run_mask2former_inference(images_state, conf_threshold, progress=gr.Progress()):
"""Runs sliding-window inference with probability accumulation and generates a COCO RLE JSON file."""
empty_json = {"error": "No image loaded."}
if not images_state:
return None, [], [], "No image available to run segmentation.", "0.00%", empty_json, None
progress(0.1, desc="Loading image onto GPU...")
# Load full-resolution image from state
original_image = images_state[0]["orig_full"]
img = np.array(original_image)
target_h, target_w = img.shape[:2]
total_pixels = target_h * target_w
img_display = (img - img.min()) / (img.max() - img.min() + 1e-8)
device = torch.device("cuda" if torch.cuda.is_available() else "cpu")
model.to(device)
model.eval()
num_classes = len(ID_TO_LABEL)
# Accumulation matrices for maximum probability projection
accumulated_probs = np.zeros((num_classes, target_h, target_w), dtype=np.float32)
class_max_confs = {cid: 0.0 for cid in range(num_classes)}
y_steps = list(range(0, target_h, STRIDE))
x_steps = list(range(0, target_w, STRIDE))
total_steps = max(1, len(y_steps) * len(x_steps))
step_counter = 0
# Sliding Window Processing Loop
for y_min in y_steps:
y_max = min(y_min + WINDOW_SIZE, target_h)
if (y_max - y_min) < 64:
continue
for x_min in x_steps:
x_max = min(x_min + WINDOW_SIZE, target_w)
if (x_max - x_min) < 64:
continue
step_counter += 1
progress(
0.1 + 0.7 * (step_counter / total_steps),
desc=f"Segmenting patch {step_counter}/{total_steps}..."
)
crop_image = original_image.crop((x_min, y_min, x_max, y_max))
crop_h, crop_w = crop_image.size[1], crop_image.size[0]
inputs = processor(images=crop_image, return_tensors="pt").to(device)
with torch.no_grad():
outputs = model(**inputs)
# Softmax class queries (exclude background channel :-1)
probas = outputs.class_queries_logits.softmax(-1)[0, :, :-1].cpu()
mask_logits = outputs.masks_queries_logits[0].cpu()
for i in range(len(probas)):
confidences = probas[i]
max_conf = confidences.max().item()
raw_class_id = confidences.argmax().item()
# Align 1-based or 0-based label indices
if raw_class_id in ID_TO_LABEL:
class_id = raw_class_id
elif (raw_class_id - 1) in ID_TO_LABEL:
class_id = raw_class_id - 1
else:
continue
display_label = ID_TO_LABEL[class_id]
if max_conf >= conf_threshold and SHOW_CATEGORIES.get(display_label, True):
m_logit = mask_logits[i].unsqueeze(0).unsqueeze(0)
m_resized = F.interpolate(
m_logit, size=(crop_h, crop_w), mode="bilinear", align_corners=False
)
prob_map = m_resized.sigmoid().squeeze().numpy() * max_conf
# Maximum Continuous Probability Accumulation
accumulated_probs[class_id, y_min:y_max, x_min:x_max] = np.maximum(
accumulated_probs[class_id, y_min:y_max, x_min:x_max],
prob_map
)
class_max_confs[class_id] = max(class_max_confs[class_id], max_conf)
progress(0.85, desc="Generating COCO RLE masks and plotting boundaries...")
# Data Structure for JSON Output
json_output = {
"image_metadata": {
"width": int(target_w),
"height": int(target_h),
"total_pixels": int(total_pixels),
"confidence_threshold_used": float(conf_threshold)
},
"summary": {
"total_coverage_pct": 0.0,
"detected_classes_count": 0
},
"annotations": []
}
# Render Visualization via Matplotlib
fig, ax = plt.subplots(figsize=(14, 10))
ax.imshow(img_display)
label_x_position = target_w + (target_w * 0.03)
ax.set_xlim(0, target_w + (target_w * 0.25))
ax.set_ylim(target_h, 0)
valid_predictions = []
for class_id in range(num_classes):
prob_map = accumulated_probs[class_id]
mask_binary = prob_map >= 0.5 # Binarize accumulated probabilities
if np.any(mask_binary):
valid_predictions.append((class_id, class_max_confs[class_id], mask_binary))
def get_top_y_coordinate(item):
mask_binary = item[2]
y_indices, _ = np.where(mask_binary)
return y_indices.min() if len(y_indices) > 0 else target_h
valid_predictions.sort(key=get_top_y_coordinate)
start_y = target_h * 0.05
y_spacing = target_h * 0.05
combined_total_mask = np.zeros((target_h, target_w), dtype=bool)
coverage_stats = []
for idx, (class_id, max_conf, mask_binary) in enumerate(valid_predictions):
display_name = ID_TO_LABEL.get(class_id, f"Class {class_id}")
color = CLASS_COLORS[class_id % len(CLASS_COLORS)]
# Calculate bounding box [xmin, ymin, width, height]
y_indices, x_indices = np.where(mask_binary)
bbox = [
int(x_indices.min()),
int(y_indices.min()),
int(x_indices.max() - x_indices.min()),
int(y_indices.max() - y_indices.min())
]
class_pixel_count = int(np.sum(mask_binary))
class_coverage_pct = round((class_pixel_count / total_pixels) * 100, 2)
# Convert Binary Mask to COCO RLE
rle_mask = binary_mask_to_rle(mask_binary)
# Append COCO Annotation to JSON output
json_output["annotations"].append({
"id": idx + 1,
"category_id": int(class_id),
"category_name": display_name,
"score": round(float(max_conf), 4),
"area": class_pixel_count,
"coverage_percentage": class_coverage_pct,
"bbox": bbox,
"segmentation": rle_mask
})
# Draw smooth contours on overlay
ax.contour(mask_binary, levels=[0.5], colors=[color], linewidths=2.0)
assigned_y_position = start_y + (idx * y_spacing)
ax.text(
label_x_position, assigned_y_position, f"{display_name} ({max_conf:.2f})",
color='white', fontsize=10, fontweight='bold', ha='left', va='center',
bbox=dict(facecolor=color, alpha=0.8, edgecolor='none', boxstyle='round,pad=0.5')
)
combined_total_mask |= mask_binary
coverage_stats.append(f"• {display_name}: {class_coverage_pct:.2f}%")
ax.axis('off')
plt.title("Mask2Former Auto-Segmentation Results", fontsize=12, pad=15)
plt.tight_layout()
buf = io.BytesIO()
plt.savefig(buf, format='png', bbox_inches='tight', dpi=150)
plt.close('all')
buf.seek(0)
segmented_pil = Image.open(buf).convert("RGB")
fw, fh = segmented_pil.size
low_w = 800
low_h = int(fh * (800 / fw))
low_segmented = segmented_pil.resize((low_w, low_h), Image.Resampling.LANCZOS)
total_covered_pixels = int(np.sum(combined_total_mask))
total_coverage_pct = round((total_covered_pixels / total_pixels) * 100, 2)
json_output["summary"]["total_coverage_pct"] = total_coverage_pct
json_output["summary"]["detected_classes_count"] = len(valid_predictions)
# Write temporary file for download button
json_filepath = "segmentation_results.json"
with open(json_filepath, "w") as f:
json.dump(json_output, f, indent=2)
coverage_report = f"Total Coverage: {total_coverage_pct:.2f}%\n" + "\n".join(coverage_stats)
if total_coverage_pct == 0:
coverage_report = "Total Coverage: 0.00%\nNo segments detected."
progress(1.0, desc="Segmentation complete!")
images_state[0]["disp_low"] = low_segmented
images_state[0]["disp_full"] = segmented_pil
return (
low_segmented,
images_state,
[], # Clear manual annotations on fresh auto-segment
"Mask2Former auto-segmentation completed.",
coverage_report,
json_output, # Rendered in gr.JSON viewer
json_filepath # Output file path for gr.File / DownloadButton
)
# ==========================================
# 4. GRADIO ANNOTATION & INTERFACE HELPERS
# ==========================================
def load_and_crop_images(files, progress=gr.Progress()):
"""Loads and crops images to a 3:2 ratio with low-res scaling for interactive responsiveness."""
if not files:
return None, [], [], "No images uploaded."
processed_images = []
target_ratio = 3 / 2
file_list = files if isinstance(files, list) else [files]
total_files = len(file_list)
for idx, f in enumerate(file_list):
progress((idx / total_files) * 0.5, desc=f"Loading image {idx + 1}/{total_files}...")
if isinstance(f, Image.Image):
pil_img = f.convert("RGB")
else:
file_path = f.name if hasattr(f, "name") else f
pil_img = Image.open(file_path).convert("RGB")
img = np.array(pil_img)
h, w, _ = img.shape
if w / h > target_ratio:
target_w = int(h * target_ratio)
target_h = h
else:
target_w = w
target_h = int(w / target_ratio)
start_x = (w - target_w) // 2
start_y = (h - target_h) // 2
cropped_img = img[start_y: start_y + target_h, start_x: start_x + target_w]
full_img = Image.fromarray(cropped_img)
progress(((idx + 0.5) / total_files), desc=f"Scaling image {idx + 1}/{total_files} to 800px...")
fw, fh = full_img.size
if fw > 800:
low_w = 800
low_h = int(fh * (800 / fw))
low_img = full_img.resize((low_w, low_h), Image.Resampling.LANCZOS)
else:
low_img = full_img.copy()
processed_images.append({
"orig_low": low_img,
"orig_full": full_img,
"disp_low": low_img.copy(),
"disp_full": full_img.copy()
})
first_low_res = processed_images[0]["disp_low"] if processed_images else None
return first_low_res, processed_images, [], f"Loaded {len(processed_images)} image(s) cropped to 3:2."
def add_to_dropdown(new_text, current_value, dropdown_component):
choices = getattr(dropdown_component, "choices", None)
if not isinstance(choices, list) or not choices:
choices = ["Spore", "Spores", "Hyphae", "Conidial Head"]
updated_choices = list(choices)
if new_text and new_text.strip():
clean_text = new_text.strip()
if clean_text not in updated_choices:
updated_choices.append(clean_text)
return gr.update(choices=updated_choices, value=clean_text, interactive=True), ""
return gr.update(choices=updated_choices, value=current_value, interactive=True), ""
def draw_annotations_on_image(base_pil_img, annotations, scale_factor=1.0):
annotated_img = np.array(base_pil_img).copy()
height, width, _ = annotated_img.shape
arrow_length = int(width * 0.05)
head_size = max(8, int(width * 0.012))
line_width = max(2, int(width * 0.003))
font_scale = max(0.6, width * 0.0008)
font_face = cv2.FONT_HERSHEY_SIMPLEX
font_thickness = max(1, int(width * 0.0015))
red_color = (255, 0, 0)
for ann in annotations:
x = int(ann["canvas_x"] * scale_factor)
y = int(ann["canvas_y"] * scale_factor)
current_label = ann["label"]
position_mode = ann["position_mode"]
if position_mode == "Top Left":
arrow_back_x, arrow_back_y = x - arrow_length, y - arrow_length
head_poly = np.array([[x, y], [x - head_size, y], [x, y - head_size]], np.int32)
elif position_mode == "Top Right":
arrow_back_x, arrow_back_y = x + arrow_length, y - arrow_length
head_poly = np.array([[x, y], [x + head_size, y], [x, y - head_size]], np.int32)
elif position_mode == "Bottom Left":
arrow_back_x, arrow_back_y = x - arrow_length, y + arrow_length
head_poly = np.array([[x, y], [x - head_size, y], [x, y + head_size]], np.int32)
else:
arrow_back_x, arrow_back_y = x + arrow_length, y + arrow_length
head_poly = np.array([[x, y], [x + head_size, y], [x, y + head_size]], np.int32)
cv2.line(annotated_img, (arrow_back_x, arrow_back_y), (x, y), red_color, thickness=line_width, lineType=cv2.LINE_AA)
cv2.fillPoly(annotated_img, [head_poly], red_color)
label_str = str(current_label)
(text_w, text_h), _ = cv2.getTextSize(label_str, font_face, font_scale, font_thickness)
padding = int(text_h * 0.4)
text_x = arrow_back_x - text_w - padding if "Left" in position_mode else arrow_back_x + padding
text_y = arrow_back_y if "Top" in position_mode else arrow_back_y + text_h + padding
bg_rect_pt1 = (text_x - padding, text_y - text_h - padding)
bg_rect_pt2 = (text_x + text_w + padding, text_y + padding // 2)
cv2.rectangle(annotated_img, bg_rect_pt1, bg_rect_pt2, (240, 240, 240), -1)
cv2.rectangle(annotated_img, bg_rect_pt1, bg_rect_pt2, (0, 0, 0), thickness=max(1, line_width // 2))
cv2.putText(annotated_img, label_str, (text_x, text_y), font_face, font_scale, red_color, thickness=font_thickness, lineType=cv2.LINE_AA)
return Image.fromarray(annotated_img)
def handle_image_click(evt: gr.SelectData, img, current_label, position_mode, current_annotations, images_state):
if not images_state:
return img, "No image loaded", current_annotations
if current_annotations is None:
current_annotations = []
x, y = evt.index[0], evt.index[1]
disp_low = images_state[0]["disp_low"]
current_annotations.append({
"canvas_x": x,
"canvas_y": y,
"label": current_label,
"position_mode": position_mode
})
annotated_img = draw_annotations_on_image(disp_low, current_annotations, scale_factor=1.0)
log_msg = f"Labeled '{current_label}' at click target ({x}, {y})."
return annotated_img, log_msg, current_annotations
def render_full_resolution(images_state, current_annotations, progress=gr.Progress()):
if not images_state:
return None, "No image loaded to render."
progress(0.2, desc="Rendering high-resolution vector overlay...")
disp_low = images_state[0]["disp_low"]
disp_full = images_state[0]["disp_full"]
scale_factor = disp_full.width / disp_low.width
annotated_full_res = draw_annotations_on_image(disp_full, current_annotations, scale_factor=scale_factor)
progress(1.0, desc="Rendering complete!")
return annotated_full_res, f"Rendered Full Scale Image ({disp_full.width}x{disp_full.height})."
# ==========================================
# 5. GRADIO APP LAYOUT & EVENT BINDINGS
# ==========================================
with gr.Blocks(title="Mask2Former Mold Segmentation Workspace") as demo:
gr.Markdown("# Interactive Image Segmentation & Annotation Workspace")
gr.Markdown("Fine-tuned Mask2Former mycology auto-segmentation with continuous probability accumulation & COCO RLE JSON exports.")
annotations_state = gr.State([])
images_state = gr.State([])
with gr.Row():
with gr.Column(scale=2):
annot_image = gr.Image(
type="pil",
format="png",
height=533,
show_label=False,
interactive=True,
)
with gr.Row():
segment_btn = gr.Button("Run Mask2Former Auto-Segmentation", variant="primary")
conf_threshold = gr.Slider(
minimum=0.0, maximum=1.0, value=0.50, step=0.05, label="Confidence Threshold"
)
rerender_btn = gr.Button("Render Manual Annotations in Full Scale", variant="secondary")
with gr.Column(scale=1):
image_upload = gr.Files(
file_types=["image"],
file_count="multiple",
label="Upload Images",
)
upload_status = gr.Textbox(label="Upload Status", interactive=False)
label_dropdown = gr.Dropdown(
choices=["Spore", "Spores", "Hyphae", "Conidial Head"],
value="Spores",
label="Select Manual Label",
interactive=True,
)
with gr.Row():
new_label_input = gr.Textbox(label="Add New Label", scale=2)
add_label_btn = gr.Button("Add", scale=1)
label_placement = gr.Radio(
choices=["Top Left", "Top Right", "Bottom Left", "Bottom Right"],
value="Bottom Right",
label="Label Box Position (Relative to Target)",
interactive=True,
)
with gr.Row():
click_log = gr.Textbox(label="Action Log", scale=2, interactive=False)
coverage_box = gr.Textbox(label="Model Area Coverage (%)", scale=1, interactive=False)
# --- JSON Output & File Download Section ---
gr.Markdown("---")
gr.Markdown("### COCO RLE Segmentation Metadata Output")
with gr.Row():
json_viewer = gr.JSON(label="Segmentation JSON Output")
json_download_file = gr.File(label="Download Segmentation JSON File", interactive=False)
# --- Event Bindings ---
annot_image.upload(
load_and_crop_images,
inputs=annot_image,
outputs=[annot_image, images_state, annotations_state, upload_status],
show_progress="full",
)
image_upload.change(
load_and_crop_images,
inputs=image_upload,
outputs=[annot_image, images_state, annotations_state, upload_status],
show_progress="full",
)
add_label_btn.click(
add_to_dropdown,
inputs=[new_label_input, label_dropdown, label_dropdown],
outputs=[label_dropdown, new_label_input],
show_progress="hidden",
)
annot_image.select(
handle_image_click,
inputs=[
annot_image,
label_dropdown,
label_placement,
annotations_state,
images_state,
],
outputs=[annot_image, click_log, annotations_state],
show_progress="minimal",
)
segment_btn.click(
run_mask2former_inference,
inputs=[images_state, conf_threshold],
outputs=[
annot_image,
images_state,
annotations_state,
click_log,
coverage_box,
json_viewer,
json_download_file
],
show_progress="full",
)
rerender_btn.click(
render_full_resolution,
inputs=[images_state, annotations_state],
outputs=[annot_image, click_log],
show_progress="full",
)
if __name__ == "__main__":
demo.launch() |