File size: 7,790 Bytes
fc8a1cf
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
## ======================================================== ##
## Menu : Microarray
## ======================================================== ##

# Loading data ####
load("data/microarray/SRscore_microarray.RData")
colnames_microarray <- colnames(Filter(is.numeric, SRscore_microarray))
SRscore_microarray[colnames_microarray] <- round(SRscore_microarray[colnames_microarray])

load("data/microarray/SRratio/ABA.RData")
load("data/microarray/SRratio/cold.RData")
load("data/microarray/SRratio/DC3000.RData")
load("data/microarray/SRratio/drought.RData")
load("data/microarray/SRratio/heat.RData")
load("data/microarray/SRratio/highlight.RData")
load("data/microarray/SRratio/hypoxia.RData")
load("data/microarray/SRratio/osmotic.RData")
load("data/microarray/SRratio/oxidation.RData")
load("data/microarray/SRratio/salt.RData")
load("data/microarray/SRratio/wound.RData")

load("data/microarray/Metadata/ABA.RData")
load("data/microarray/Metadata/cold.RData")
load("data/microarray/Metadata/DC3000.RData")
load("data/microarray/Metadata/drought.RData")
load("data/microarray/Metadata/heat.RData")
load("data/microarray/Metadata/highlight.RData")
load("data/microarray/Metadata/hypoxia.RData")
load("data/microarray/Metadata/osmotic.RData")
load("data/microarray/Metadata/oxidation.RData")
load("data/microarray/Metadata/salt.RData")
load("data/microarray/Metadata/wound.RData")

# Color settings of a heatmap ####
paletteLength <- 100
microarrayBreaks <- c(seq(min(Filter(is.numeric, SRscore_microarray)), 0, length.out = (paletteLength/2)), 
              seq(0, max(Filter(is.numeric, SRscore_microarray)), length.out = (paletteLength/2))[-1])
microarrayColor <- colorRampPalette(c("deepskyblue", "white", "hotpink"))(paletteLength)

# Links to external databases ####
link <- rep("link", nrow(SRscore_microarray))

## AlphaFold2
url <- rep(paste0("https://alphafold.ebi.ac.uk/search/text/",
                  SRscore_microarray$ensembl_gene_id))
AF2 <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## ATTED-II
url <- rep(paste0("https://atted.jp/kwsearch/?stype=any&kword=", 
                  SRscore_microarray$ensembl_gene_id, 
                  "&searchBtnK.x=0&searchBtnK.y=0"))
ATTED2 <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## eFP Browser
url <- rep(paste0("https://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi?dataSource=Abiotic_Stress&mode=Absolute&primaryGene=",
                  SRscore_microarray$ensembl_gene_id))
eFP <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## KEGG
url <- rep(paste0("https://www.genome.jp/dbget-bin/www_bget?ath:", SRscore_microarray$ensembl_gene_id))
KEGG <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## STRING
url <- rep(paste0("https://string-db.org/cgi/network?identifiers=",
                  SRscore_microarray$ensembl_gene_id,
                  "&species=3702&show_query_node_labels=1"))
STRING <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## TAIR
url <- rep(paste0("https://www-arabidopsis-org.translate.goog/servlets/TairObject?type=locus&name=", 
                  SRscore_microarray$ensembl_gene_id, 
                  "&_x_tr_sl=en&_x_tr_tl=ja&_x_tr_hl=ja&_x_tr_pto=sc"))
TAIR <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## ThaleMine
url <- rep(paste0("https://bar.utoronto.ca/thalemine/keywordSearchResults.do?searchTerm=",
                  SRscore_microarray$ensembl_gene_id, "&searchSubmit=GO"))
TM <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## Joining links to the atlas
SRscore_microarray <- cbind(SRscore_microarray, "AlphaFold2" = AF2, "ATTED-II" = ATTED2, "eFP Browser" = eFP, 
                         "KEGG" = KEGG, "STRING" = STRING, "TAIR" = TAIR, "Thale Mine" = TM)

## Convert the atlas to template matching format 
genefinder_microarray <- column_to_rownames(SRscore_microarray, var = "ensembl_gene_id")
genefinder_microarray <- Filter(is.numeric, genefinder_microarray)
genefinder_microarray <- as.matrix(genefinder_microarray)


## ========================================================= ##
## Menu : RNA-Seq
## ========================================================= ##

# Loading data ####
load("data/rnaseq/SRscore_rnaseq.RData")
colnames_rnaseq <- colnames(Filter(is.numeric, SRscore_rnaseq))
SRscore_rnaseq[colnames_rnaseq] <- round(SRscore_rnaseq[colnames_rnaseq])

load("data/rnaseq/SRratio/ABA.RData")
load("data/rnaseq/SRratio/cold.RData")
load("data/rnaseq/SRratio/DC3000.RData")
load("data/rnaseq/SRratio/drought.RData")
load("data/rnaseq/SRratio/heat.RData")
load("data/rnaseq/SRratio/highlight.RData")
load("data/rnaseq/SRratio/hypoxia.RData")
load("data/rnaseq/SRratio/osmotic.RData")
load("data/rnaseq/SRratio/oxidation.RData")
load("data/rnaseq/SRratio/salt.RData")
load("data/rnaseq/SRratio/wound.RData")

load("data/rnaseq/Metadata/ABA.RData")
load("data/rnaseq/Metadata/cold.RData")
load("data/rnaseq/Metadata/DC3000.RData")
load("data/rnaseq/Metadata/drought.RData")
load("data/rnaseq/Metadata/heat.RData")
load("data/rnaseq/Metadata/highlight.RData")
load("data/rnaseq/Metadata/hypoxia.RData")
load("data/rnaseq/Metadata/osmotic.RData")
load("data/rnaseq/Metadata/oxidation.RData")
load("data/rnaseq/Metadata/salt.RData")
load("data/rnaseq/Metadata/wound.RData")

# Color settings of a heatmap ####
paletteLength <- 100
rnaseqBreaks <- c(seq(min(Filter(is.numeric, SRscore_rnaseq)), 0, length.out = (paletteLength/2)), 
                  seq(0, max(Filter(is.numeric, SRscore_rnaseq)), length.out = (paletteLength/2))[-1])
rnaseqColor <- colorRampPalette(c("deepskyblue", "white", "hotpink"))(paletteLength)

# Links to external databases ####
link <- rep("link", nrow(SRscore_rnaseq))

## AlphaFold2
url <- rep(paste0("https://alphafold.ebi.ac.uk/search/text/",
                  SRscore_rnaseq$ensembl_gene_id))
AF2 <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## ATTED-II
url <- rep(paste0("https://atted.jp/kwsearch/?stype=any&kword=", 
                  SRscore_rnaseq$ensembl_gene_id, 
                  "&searchBtnK.x=0&searchBtnK.y=0"))
ATTED2 <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## eFP Browser
url <- rep(paste0("https://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi?dataSource=Abiotic_Stress&mode=Absolute&primaryGene=",
                  SRscore_rnaseq$ensembl_gene_id))
eFP <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## KEGG
url <- rep(paste0("https://www.genome.jp/dbget-bin/www_bget?ath:", SRscore_rnaseq$ensembl_gene_id))
KEGG <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## STRING
url <- rep(paste0("https://string-db.org/cgi/network?identifiers=",
                  SRscore_rnaseq$ensembl_gene_id,
                  "&species=3702&show_query_node_labels=1"))
STRING <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## TAIR
url <- rep(paste0("https://www-arabidopsis-org.translate.goog/servlets/TairObject?type=locus&name=", 
                  SRscore_rnaseq$ensembl_gene_id, 
                  "&_x_tr_sl=en&_x_tr_tl=ja&_x_tr_hl=ja&_x_tr_pto=sc"))
TAIR <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

## ThaleMine
url <- rep(paste0("https://bar.utoronto.ca/thalemine/keywordSearchResults.do?searchTerm=",
                  SRscore_rnaseq$ensembl_gene_id, "&searchSubmit=GO"))
TM <- paste0("<a href = ", "'", url, "'", ">", link, "</a>")

# Joining links to the atlas
SRscore_rnaseq <- cbind(SRscore_rnaseq, "AlphaFold2" = AF2, "ATTED-II" = ATTED2, "eFP Browser" = eFP,
                     "KEGG" = KEGG, "STRING" = STRING, "TAIR" = TAIR, "Thale Mine" = TM)

# Convert the atlas to template matching format 
genefinder_rnaseq <- column_to_rownames(SRscore_rnaseq, var = "ensembl_gene_id")
genefinder_rnaseq <- Filter(is.numeric, genefinder_rnaseq)
genefinder_rnaseq <- as.matrix(genefinder_rnaseq)