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Update app.py
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app.py
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@@ -9,6 +9,7 @@ from huggingface_hub import hf_hub_download
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# ---------------------------
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# Device configuration
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# ---------------------------
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device = torch.device("cuda" if torch.cuda.is_available() else "cpu")
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# ---------------------------
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@@ -16,7 +17,7 @@ device = torch.device("cuda" if torch.cuda.is_available() else "cpu")
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# ---------------------------
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def build_model():
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"""Re
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backbone = timm.create_model(
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"convnext_small", pretrained=False, num_classes=0, global_pool="avg"
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)
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@@ -57,19 +58,29 @@ transform = transforms.Compose(
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# ---------------------------
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# Inference function
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# ---------------------------
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THRESHOLD = 0.5 # adjust if you want to tweak the decision boundary
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def predict(img: Image.Image, magnification: int, ra_conc: float):
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"""Return probabilities for High / Low CPM classes.
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"""
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img_tensor = transform(img).unsqueeze(0).to(device)
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with torch.no_grad():
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logit = model(img_tensor)
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prob_low = 1.0 - prob_high
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@@ -89,13 +100,21 @@ demo = gr.Interface(
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gr.Image(type="pil", label="Microscopy Image"),
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gr.Dropdown(choices=[4, 10, 20], value=10, label="Magnification (×)"),
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gr.Dropdown(choices=[0.1, 0.5, 1.0], value=0.1, label="RA Concentration (µM)"),
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],
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outputs=gr.Label(num_top_classes=2, label="Predicted CPM Class & Probability"),
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title="iPS Cell Quality Classifier",
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description=(
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"Upload a microscopy image, choose magnification
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"(
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"
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),
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# ---------------------------
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# Device configuration
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# ---------------------------
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device = torch.device("cuda" if torch.cuda.is_available() else "cpu")
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# ---------------------------
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# ---------------------------
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def build_model():
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"""Re-create the network architecture used during training."""
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backbone = timm.create_model(
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"convnext_small", pretrained=False, num_classes=0, global_pool="avg"
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)
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# ---------------------------
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# Inference function
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# ---------------------------
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def predict(
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img: Image.Image,
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magnification: int,
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ra_conc: float,
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temperature: float = 1.0,
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):
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"""Return probabilities for High/Low CPM classes with optional temperature scaling.
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Args:
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img: Microscopy image.
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magnification: Tag for objective magnification (×4/10/20).
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ra_conc: Tag for RA concentration (µM).
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temperature: Temperature parameter for confidence calibration. T>1 lowers
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confidence, T<1 increases confidence.
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"""
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img_tensor = transform(img).unsqueeze(0).to(device)
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with torch.no_grad():
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logit = model(img_tensor)
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# Temperature scaling for calibration
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logit_scaled = logit / temperature
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prob_high = torch.sigmoid(logit_scaled).item()
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prob_low = 1.0 - prob_high
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gr.Image(type="pil", label="Microscopy Image"),
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gr.Dropdown(choices=[4, 10, 20], value=10, label="Magnification (×)"),
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gr.Dropdown(choices=[0.1, 0.5, 1.0], value=0.1, label="RA Concentration (µM)"),
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gr.Slider(
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minimum=0.5,
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maximum=5.0,
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step=0.1,
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value=1.0,
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label="Temperature (confidence calibration)",
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info="Increase temperature to reduce overconfidence",
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),
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],
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outputs=gr.Label(num_top_classes=2, label="Predicted CPM Class & Probability"),
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title="iPS Cell Quality Classifier",
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description=(
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"Upload a microscopy image, choose magnification & RA concentration "
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"(metadata only), then optionally adjust the *Temperature* slider to "
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"calibrate confidence if predictions look over‑ or under‑confident."
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),
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)
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