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app.py

@@ -683,14 +683,15 @@ def parse_xls_file(raw_bytes):
     pra6 = pra_all['X6']
     overall = 'Positive' if pra6 > 0 else 'Negative'
 
-    # Confident alleles (sero 層級檢查)
-    # 將 beads_detail 轉為 get_confident_alleles 需要的格式
+    # Confident alleles — XLS 路徑：用 XLS 自帶的 allele 建 bead map（lot 可能不同）
     br_dict = OrderedDict()
+    xls_bead_map = {}
     for b in beads_detail:
         br_dict[b['bead']] = {'rxn': b['rxn']}
-    confident, allele_decisions = get_confident_alleles(br_dict, bead_map)
+        xls_bead_map[b['bead']] = {'sero': b['sero'], 'allele': b['allele']}
+    confident, allele_decisions = get_confident_alleles(br_dict, xls_bead_map)
 
-    sero_mfi = build_sero_mfi_stats(beads_detail, confident, bead_map) if overall == 'Positive' else []
+    sero_mfi = build_sero_mfi_stats(beads_detail, confident, xls_bead_map) if overall == 'Positive' else []
 
     pc_nc_ratio = round(pc_raw / nc_raw, 1) if nc_raw > 0 else 0
     qc_comment = build_qc_comment(nc_raw, pc_raw, beads_detail)
@@ -703,7 +704,7 @@ def parse_xls_file(raw_bytes):
         'beads': beads_detail,
         'confident_alleles': sorted(confident),
         'allele_decisions': allele_decisions,
-        'specificity': generate_specificity(confident, bead_map) if overall == 'Positive' else '(-)',
+        'specificity': generate_specificity(confident, xls_bead_map) if overall == 'Positive' else '(-)',
         'sero_mfi': sero_mfi,
         'comment': qc_comment,
         'qc': {