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+# TransVar API for HuggingFace Spaces
+# 从 GitHub 仓库自动构建
+# 仓库: https://github.com/pzweuj/TransVar2API
+
+FROM python:3.9-slim
+
+# 设置环境变量
+ENV DEBIAN_FRONTEND=noninteractive \
+    TRANSVAR_DB_PATH=/data/transvar_db \
+    PYTHONUNBUFFERED=1
+
+# 安装系统依赖
+RUN apt-get update && apt-get install -y \
+    wget \
+    curl \
+    git \
+    samtools \
+    build-essential \
+    zlib1g-dev \
+    libbz2-dev \
+    liblzma-dev \
+    libcurl4-gnutls-dev \
+    && rm -rf /var/lib/apt/lists/*
+
+# 从 GitHub 克隆仓库
+WORKDIR /app
+RUN git clone https://github.com/pzweuj/TransVar2API.git . \
+    || git clone https://github.com/pzweuj/TransVar2API.git /tmp/repo && cp -r /tmp/repo/* /app/ && cp -r /tmp/repo/.* /app/ 2>/dev/null || true
+
+# 安装 Python 依赖
+RUN pip3 install --no-cache-dir -r requirements.txt
+
+# 安装 transvar
+RUN pip3 install --no-cache-dir transvar
+
+# 创建数据目录
+RUN mkdir -p /data/transvar_db/refseq_hg38 /data/transvar_db/refseq_hg19
+
+# ========== 构建 hg38 数据库 ==========
+WORKDIR /data/transvar_db/refseq_hg38
+
+# 下载 hg38 参考基因组
+RUN echo "Downloading hg38 reference genome..." && \
+    wget -q -O hg38.fa.gz https://hgdownload.soe.ucsc.edu/goldenPath/hg38/bigZips/hg38.fa.gz && \
+    gunzip -f hg38.fa.gz && \
+    samtools faidx hg38.fa
+
+# 下载 hg38 RefSeq 注释并构建索引
+RUN echo "Building hg38 transvar index..." && \
+    wget -q -O ncbiRefSeq.txt.gz https://hgdownload.soe.ucsc.edu/goldenPath/hg38/database/ncbiRefSeq.txt.gz && \
+    transvar index --refseq ncbiRefSeq.txt.gz && \
+    transvar config -k reference -v /data/transvar_db/refseq_hg38/hg38.fa --refversion hg38_refseq && \
+    transvar config -k refseq -v /data/transvar_db/refseq_hg38/ncbiRefSeq.txt.gz --refversion hg38_refseq
+
+# ========== 构建 hg19 数据库 ==========
+WORKDIR /data/transvar_db/refseq_hg19
+
+# 下载 hg19 参考基因组
+RUN echo "Downloading hg19 reference genome..." && \
+    wget -q -O hg19.fa.gz https://hgdownload.soe.ucsc.edu/goldenPath/hg19/bigZips/hg19.fa.gz && \
+    gunzip -f hg19.fa.gz && \
+    samtools faidx hg19.fa
+
+# 下载 hg19 RefSeq 注释并构建索引
+RUN echo "Building hg19 transvar index..." && \
+    wget -q -O ncbiRefSeq.txt.gz https://hgdownload.soe.ucsc.edu/goldenPath/hg19/database/ncbiRefSeq.txt.gz && \
+    transvar index --refseq ncbiRefSeq.txt.gz && \
+    transvar config -k reference -v /data/transvar_db/refseq_hg19/hg19.fa --refversion hg19_refseq && \
+    transvar config -k refseq -v /data/transvar_db/refseq_hg19/ncbiRefSeq.txt.gz --refversion hg19_refseq
+
+# 返回工作目录
+WORKDIR /app
+
+# 暴露 HuggingFace Spaces 默认端口
+EXPOSE 7860
+
+# HuggingFace Spaces 环境变量
+ENV PORT=7860
+
+# 启动命令 - 使用环境变量 PORT
+CMD python3 -c "import os; import uvicorn; from server import app; uvicorn.run(app, host='0.0.0.0', port=int(os.environ.get('PORT', 7860)))"