Update AEON inference to require sex parameter

- Remove 'Unknown' from sex choices in CLI arguments
- Update docstrings to indicate sex is required
- Clarify that sex must be 'Male' or 'Female' for inference

src/mosaic/analysis.py

@@ -673,7 +673,7 @@ def analyze_slide(
         slide_path: Path to the whole slide image file
         seg_config: Segmentation configuration, one of "Biopsy", "Resection", or "TCGA"
         site_type: Site type, either "Primary" or "Metastatic"
-        sex: Patient sex ("Unknown", "Male", "Female")
+        sex: Patient sex ("Male" or "Female") - required
         tissue_site: Tissue site name
         cancer_subtype: Cancer subtype (OncoTree code or "Unknown" for inference)
         cancer_subtype_name_map: Dictionary mapping cancer subtype names to codes

src/mosaic/inference/aeon.py

@@ -224,9 +224,9 @@ def parse_args():
     parser.add_argument(
         "--sex",
         type=str,
-        choices=["Male", "Female", "Unknown"],
+        choices=["Male", "Female"],
         default=None,
-        help="Patient sex (Male or Female)",
+        help="Patient sex (Male or Female, required for inference)",
     )
     parser.add_argument(
         "--tissue-site",