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{
  "stages": [
    {
      "sheet": "1. Raw Material",
      "title": "STAGE 1 \u2014 Raw-material candidate characterization",
      "nFields": 23,
      "nMinimum": 10,
      "nIdeal": 13,
      "fields": [
        {
          "id": "RM-01",
          "name": "raw_material_id",
          "subsection": "Identity & sourcing",
          "description": "Unique identifier for the raw-material sample; primary key linking to downstream tables.",
          "tier": "Minimum",
          "example": "RM_001"
        },
        {
          "id": "RM-02",
          "name": "organism_species",
          "subsection": "Identity & sourcing",
          "description": "Source organism (scientific name) the material is derived from.",
          "tier": "Minimum",
          "example": "<genus species>"
        },
        {
          "id": "RM-03",
          "name": "organism_ncbi_taxid",
          "subsection": "Identity & sourcing",
          "description": "NCBI Taxonomy identifier for the source organism (machine-readable).",
          "tier": "Ideal",
          "example": "7370"
        },
        {
          "id": "RM-04",
          "name": "tissue_or_fraction",
          "subsection": "Identity & sourcing",
          "description": "Specific tissue / anatomical fraction of the organism extracted.",
          "tier": "Minimum",
          "example": "whole body"
        },
        {
          "id": "RM-05",
          "name": "supplier",
          "subsection": "Identity & sourcing",
          "description": "Supplier / vendor of the raw material.",
          "tier": "Minimum",
          "example": "<supplier>"
        },
        {
          "id": "RM-06",
          "name": "batch_lot_id",
          "subsection": "Identity & sourcing",
          "description": "Supplier batch / lot number for traceability across process batches.",
          "tier": "Minimum",
          "example": "LOT-2024-08"
        },
        {
          "id": "RM-07",
          "name": "material_form",
          "subsection": "Pretreatment",
          "description": "Form of the input material (governs baseline protein concentration).",
          "tier": "Minimum",
          "example": "isolate"
        },
        {
          "id": "RM-08",
          "name": "defatting_method",
          "subsection": "Pretreatment",
          "description": "Defatting / lipid-removal method and conditions, if applied.",
          "tier": "Ideal",
          "example": "hexane extraction"
        },
        {
          "id": "RM-09",
          "name": "thermal_pretreatment",
          "subsection": "Pretreatment",
          "description": "Thermal denaturation / cooking step and conditions, if applied.",
          "tier": "Ideal",
          "example": "90 \u00b0C, 10 min"
        },
        {
          "id": "RM-10",
          "name": "other_pretreatment",
          "subsection": "Pretreatment",
          "description": "Any additional upstream pretreatment steps (e.g. pH shift, milling).",
          "tier": "Ideal",
          "example": "pH shift"
        },
        {
          "id": "RM-11",
          "name": "total_protein_content",
          "subsection": "Composition & abundance",
          "description": "Total protein content; reference value for the sample.",
          "tier": "Minimum",
          "example": "62.4"
        },
        {
          "id": "RM-12",
          "name": "total_protein_method",
          "subsection": "Composition & abundance",
          "description": "Method used for total-protein quantification (AA analysis is the reference method).",
          "tier": "Minimum",
          "example": "AA analysis"
        },
        {
          "id": "RM-13",
          "name": "aa_composition",
          "subsection": "Composition & abundance",
          "description": "Amino-acid composition profile of the raw material.",
          "tier": "Minimum",
          "example": "per-AA table"
        },
        {
          "id": "RM-14",
          "name": "reference_proteome_available",
          "subsection": "Composition & abundance",
          "description": "Whether a well-annotated reference proteome exists for the species (gates value of LC-MS/MS abundance data).",
          "tier": "Minimum",
          "example": "TRUE"
        },
        {
          "id": "RM-15",
          "name": "reference_proteome_source",
          "subsection": "Composition & abundance",
          "description": "Source and version of the reference proteome used.",
          "tier": "Ideal",
          "example": "UP0000XXXXX, 2024_03"
        },
        {
          "id": "RM-16",
          "name": "protein_abundance_profile",
          "subsection": "Composition & abundance",
          "description": "Relative protein abundance prior to hydrolysis (quantitative bottom-up proteomics).",
          "tier": "Ideal",
          "example": "per-protein table"
        },
        {
          "id": "RM-17",
          "name": "abundance_weighted_aa",
          "subsection": "Composition & abundance",
          "description": "Abundance-weighted AA composition derived from the proteomics profile; cross-check against AA analysis.",
          "tier": "Ideal",
          "example": "derived"
        },
        {
          "id": "RM-18",
          "name": "abundance_deposit_id",
          "subsection": "Composition & abundance",
          "description": "Identifier of the deposited abundance dataset in a tissue/organism abundance meta-resource (e.g. PaxDB).",
          "tier": "Ideal",
          "example": "see Reference Resources"
        },
        {
          "id": "RM-19",
          "name": "proxy_quant_method",
          "subsection": "Composition & abundance",
          "description": "Cheaper proxy protein-quant method, correlated to AA analysis, for routine QA/QC.",
          "tier": "Ideal",
          "example": "Dumas"
        },
        {
          "id": "RM-20",
          "name": "proxy_quant_value",
          "subsection": "Composition & abundance",
          "description": "Value returned by the proxy quantification method.",
          "tier": "Ideal",
          "example": "60.1"
        },
        {
          "id": "RM-21",
          "name": "nir_spectrum",
          "subsection": "Composition & abundance",
          "description": "NIR spectrum for rapid/affordable QA/QC; can be matched to AA composition via PLS modelling.",
          "tier": "Ideal",
          "example": "spectrum file"
        },
        {
          "id": "RM-22",
          "name": "expected_contaminants",
          "subsection": "Contaminants",
          "description": "Contaminant proteins/compounds expected for this material and planned for filtering.",
          "tier": "Ideal",
          "example": "keratin; trypsin"
        },
        {
          "id": "RM-23",
          "name": "contaminant_library_ref",
          "subsection": "Contaminants",
          "description": "Reference contaminant library used in the proteomics workflow.",
          "tier": "Ideal",
          "example": "cRAP (see References)"
        }
      ]
    },
    {
      "sheet": "2. Hydrolysis Process",
      "title": "STAGE 2 \u2014 Hydrolysis process characterization",
      "nFields": 41,
      "nMinimum": 17,
      "nIdeal": 24,
      "fields": [
        {
          "id": "HP-01",
          "name": "hydrolysate_id",
          "subsection": "Identity & linkage",
          "description": "Unique identifier for the hydrolysate batch; primary key for this stage.",
          "tier": "Minimum",
          "example": "PH_001"
        },
        {
          "id": "HP-02",
          "name": "source_raw_material_id",
          "subsection": "Identity & linkage",
          "description": "Foreign key to the raw-material sample used as substrate.",
          "tier": "Minimum",
          "example": "RM_001"
        },
        {
          "id": "HP-03",
          "name": "enzyme_name",
          "subsection": "Enzymes",
          "description": "Common / commercial name of each enzyme used.",
          "tier": "Minimum",
          "example": "Alcalase 2.4L"
        },
        {
          "id": "HP-04",
          "name": "enzyme_sequence",
          "subsection": "Enzymes",
          "description": "Amino-acid sequence of the enzyme (enables cleavage modelling).",
          "tier": "Ideal",
          "example": "MK..."
        },
        {
          "id": "HP-05",
          "name": "enzyme_uniprot",
          "subsection": "Enzymes",
          "description": "UniProt accession of the enzyme, if applicable.",
          "tier": "Ideal",
          "example": "P00780"
        },
        {
          "id": "HP-06",
          "name": "enzyme_grade",
          "subsection": "Enzymes",
          "description": "Whether the enzyme is purified or technical grade.",
          "tier": "Minimum",
          "example": "technical"
        },
        {
          "id": "HP-07",
          "name": "enzyme_primary_activity",
          "subsection": "Enzymes",
          "description": "Primary catalytic activity and associated cleavage tendencies, if known.",
          "tier": "Ideal",
          "example": "endopeptidase, broad specificity"
        },
        {
          "id": "HP-08",
          "name": "enzyme_secondary_activity",
          "subsection": "Enzymes",
          "description": "Known secondary activities (important for mixtures / technical grades).",
          "tier": "Ideal",
          "example": "high exopeptidase activity"
        },
        {
          "id": "HP-09",
          "name": "enzyme_application_mode",
          "subsection": "Enzymes",
          "description": "For multiple enzymes, whether they were applied sequentially or simultaneously.",
          "tier": "Minimum",
          "example": "sequential"
        },
        {
          "id": "HP-10",
          "name": "buffer_composition",
          "subsection": "Reaction conditions",
          "description": "Aqueous buffer composition.",
          "tier": "Minimum",
          "example": "50 mM Tris-HCl"
        },
        {
          "id": "HP-11",
          "name": "substrate_concentration",
          "subsection": "Reaction conditions",
          "description": "Concentration of substrate dissolved in the buffer.",
          "tier": "Minimum",
          "example": "5"
        },
        {
          "id": "HP-12",
          "name": "es_ratio_mass",
          "subsection": "Reaction conditions",
          "description": "Enzyme-to-substrate ratio on a protein basis.",
          "tier": "Minimum",
          "example": "2"
        },
        {
          "id": "HP-13",
          "name": "es_ratio_activity",
          "subsection": "Reaction conditions",
          "description": "Enzyme-to-substrate ratio on an activity:mass basis.",
          "tier": "Ideal",
          "example": "50"
        },
        {
          "id": "HP-14",
          "name": "activity_assay",
          "subsection": "Reaction conditions",
          "description": "Activity assay basis used to express the activity:mass ratio.",
          "tier": "Ideal",
          "example": "AU/g (Anson)"
        },
        {
          "id": "HP-15",
          "name": "temperature",
          "subsection": "Reaction conditions",
          "description": "Reaction temperature.",
          "tier": "Minimum",
          "example": "55"
        },
        {
          "id": "HP-16",
          "name": "pH",
          "subsection": "Reaction conditions",
          "description": "Reaction pH.",
          "tier": "Minimum",
          "example": "8.0"
        },
        {
          "id": "HP-17",
          "name": "pH_controlled",
          "subsection": "Reaction conditions",
          "description": "Whether pH was actively controlled during hydrolysis.",
          "tier": "Minimum",
          "example": "TRUE"
        },
        {
          "id": "HP-18",
          "name": "agitation",
          "subsection": "Reaction conditions",
          "description": "Agitation rate, or static.",
          "tier": "Ideal",
          "example": "150 rpm"
        },
        {
          "id": "HP-19",
          "name": "duration",
          "subsection": "Reaction conditions",
          "description": "Total hydrolysis duration.",
          "tier": "Minimum",
          "example": "120"
        },
        {
          "id": "HP-20",
          "name": "sampling_timepoint",
          "subsection": "Reaction conditions",
          "description": "Time point(s) at which sample(s) were extracted from the reaction.",
          "tier": "Ideal",
          "example": "120"
        },
        {
          "id": "HP-21",
          "name": "termination_method",
          "subsection": "Termination",
          "description": "Method used to terminate the reaction.",
          "tier": "Minimum",
          "example": "heat inactivation, 100 \u00b0C / 15 min"
        },
        {
          "id": "HP-22",
          "name": "degree_of_hydrolysis",
          "subsection": "Degree of hydrolysis",
          "description": "Degree of hydrolysis (DH%): fraction of peptide bonds cleaved.",
          "tier": "Minimum",
          "example": "18.5"
        },
        {
          "id": "HP-23",
          "name": "dh_assay",
          "subsection": "Degree of hydrolysis",
          "description": "Assay used for DH; choice is substrate/enzyme-specific and should be justified.",
          "tier": "Minimum",
          "example": "OPA"
        },
        {
          "id": "HP-24",
          "name": "centrifugation_gforce",
          "subsection": "Post-hydrolysis processing",
          "description": "Centrifugation g-force used to clarify the hydrolysate.",
          "tier": "Ideal",
          "example": "10000"
        },
        {
          "id": "HP-25",
          "name": "centrifugation_duration",
          "subsection": "Post-hydrolysis processing",
          "description": "Centrifugation duration.",
          "tier": "Ideal",
          "example": "20"
        },
        {
          "id": "HP-26",
          "name": "centrifugation_temperature",
          "subsection": "Post-hydrolysis processing",
          "description": "Centrifugation temperature.",
          "tier": "Ideal",
          "example": "4"
        },
        {
          "id": "HP-27",
          "name": "soluble_protein_yield",
          "subsection": "Post-hydrolysis processing",
          "description": "Soluble protein yield: how much sample ends up usable in the final product; governs how representative LC-MS/MS data are.",
          "tier": "Minimum",
          "example": "72"
        },
        {
          "id": "HP-28",
          "name": "soluble_protein_yield_method",
          "subsection": "Post-hydrolysis processing",
          "description": "Method used to determine soluble protein yield.",
          "tier": "Minimum",
          "example": "Lowry on supernatant"
        },
        {
          "id": "HP-29",
          "name": "filtration_fractionation",
          "subsection": "Post-hydrolysis processing",
          "description": "Filtration / fractionation step (e.g. membrane MWCO ultrafiltration) and parameters.",
          "tier": "Ideal",
          "example": "10 kDa UF"
        },
        {
          "id": "HP-30",
          "name": "drying_method",
          "subsection": "Post-hydrolysis processing",
          "description": "Drying method applied to the final product.",
          "tier": "Ideal",
          "example": "lyophilization"
        },
        {
          "id": "HP-31",
          "name": "mwd",
          "subsection": "Final product characterization",
          "description": "Molecular-weight distribution of the product peptidome; reference set to contrast detected peptides against.",
          "tier": "Ideal",
          "example": "distribution"
        },
        {
          "id": "HP-32",
          "name": "mwd_method",
          "subsection": "Final product characterization",
          "description": "Method and parameters for MWD measurement.",
          "tier": "Ideal",
          "example": "SEC; column details"
        },
        {
          "id": "HP-33",
          "name": "faa_content",
          "subsection": "Final product characterization",
          "description": "Free amino-acid content; constrains the set of truly undetected peptides (mass below LC-MS/MS cutoff).",
          "tier": "Ideal",
          "example": "8.2"
        },
        {
          "id": "HP-34",
          "name": "product_aa_analysis",
          "subsection": "Final product characterization",
          "description": "AA analysis (or a highly correlated proxy) of the final product.",
          "tier": "Ideal",
          "example": "per-AA table"
        },
        {
          "id": "HP-35",
          "name": "amino_nitrogen",
          "subsection": "Final product characterization",
          "description": "Amino nitrogen (AN).",
          "tier": "Ideal",
          "example": "\u2014"
        },
        {
          "id": "HP-36",
          "name": "total_nitrogen",
          "subsection": "Final product characterization",
          "description": "Total nitrogen (TN).",
          "tier": "Ideal",
          "example": "\u2014"
        },
        {
          "id": "HP-37",
          "name": "an_tn_ratio",
          "subsection": "Final product characterization",
          "description": "AN/TN ratio (derived from AN and TN).",
          "tier": "Ideal",
          "example": "\u2014"
        },
        {
          "id": "HP-38",
          "name": "ash_content",
          "subsection": "Final product characterization",
          "description": "Ash content.",
          "tier": "Ideal",
          "example": "\u2014"
        },
        {
          "id": "HP-39",
          "name": "moisture_content",
          "subsection": "Final product characterization",
          "description": "Moisture content.",
          "tier": "Ideal",
          "example": "\u2014"
        },
        {
          "id": "HP-40",
          "name": "product_pH",
          "subsection": "Final product characterization",
          "description": "pH of the final product.",
          "tier": "Ideal",
          "example": "6.8"
        },
        {
          "id": "HP-41",
          "name": "mineral_profile",
          "subsection": "Final product characterization",
          "description": "Mineral content: calcium, iron, magnesium, phosphorus, potassium, sodium.",
          "tier": "Ideal",
          "example": "per-mineral table"
        }
      ]
    },
    {
      "sheet": "3. LC-MS-MS",
      "title": "STAGE 3 \u2014 LC-MS/MS process characterization",
      "nFields": 43,
      "nMinimum": 13,
      "nIdeal": 30,
      "fields": [
        {
          "id": "MS-01",
          "name": "run_id",
          "subsection": "Identity & linkage",
          "description": "Unique identifier for the LC-MS/MS run; primary key for this stage.",
          "tier": "Minimum",
          "example": "RUN_001"
        },
        {
          "id": "MS-02",
          "name": "source_hydrolysate_id",
          "subsection": "Identity & linkage",
          "description": "Foreign key to the hydrolysate analyzed.",
          "tier": "Minimum",
          "example": "PH_001"
        },
        {
          "id": "MS-03",
          "name": "sample_prep_filtering",
          "subsection": "Sample prep & LC",
          "description": "Filtering / clean-up steps (e.g. desalting); can remove non-peptidomic content but also drop some peptides.",
          "tier": "Minimum",
          "example": "C18 desalting"
        },
        {
          "id": "MS-04",
          "name": "sample_prep_equipment",
          "subsection": "Sample prep & LC",
          "description": "Equipment / sorbent used for clean-up.",
          "tier": "Ideal",
          "example": "C18 StageTip"
        },
        {
          "id": "MS-05",
          "name": "sample_prep_conditions",
          "subsection": "Sample prep & LC",
          "description": "Conditions of the clean-up step.",
          "tier": "Ideal",
          "example": "30 min"
        },
        {
          "id": "MS-06",
          "name": "lc_column_chemistry",
          "subsection": "Sample prep & LC",
          "description": "LC column stationary-phase chemistry.",
          "tier": "Minimum",
          "example": "C18"
        },
        {
          "id": "MS-07",
          "name": "lc_column_dimensions",
          "subsection": "Sample prep & LC",
          "description": "Column dimensions and particle size.",
          "tier": "Ideal",
          "example": "75 \u00b5m \u00d7 25 cm; 1.7 \u00b5m"
        },
        {
          "id": "MS-08",
          "name": "lc_load_amount",
          "subsection": "Sample prep & LC",
          "description": "Amount loaded per column; influences expected input quantity of each peptide.",
          "tier": "Ideal",
          "example": "1"
        },
        {
          "id": "MS-09",
          "name": "lc_flow_regime",
          "subsection": "Sample prep & LC",
          "description": "Flow regime.",
          "tier": "Ideal",
          "example": "nano"
        },
        {
          "id": "MS-10",
          "name": "lc_flow_rate",
          "subsection": "Sample prep & LC",
          "description": "Flow rate.",
          "tier": "Ideal",
          "example": "300"
        },
        {
          "id": "MS-11",
          "name": "lc_gradient_length",
          "subsection": "Sample prep & LC",
          "description": "Gradient length.",
          "tier": "Ideal",
          "example": "90"
        },
        {
          "id": "MS-12",
          "name": "lc_resolution_metrics",
          "subsection": "Sample prep & LC",
          "description": "Reported LC resolution / selectivity / co-elution metrics (retention, selectivity, efficiency; separation factor).",
          "tier": "Ideal",
          "example": "separation factor"
        },
        {
          "id": "MS-13",
          "name": "ms_instrument",
          "subsection": "MS acquisition (MS1)",
          "description": "MS instrument model/type; sets transmission and detection efficiency.",
          "tier": "Minimum",
          "example": "Orbitrap"
        },
        {
          "id": "MS-14",
          "name": "acquisition_mode",
          "subsection": "MS acquisition (MS1)",
          "description": "Acquisition mode.",
          "tier": "Minimum",
          "example": "DDA"
        },
        {
          "id": "MS-15",
          "name": "ms1_scan_range",
          "subsection": "MS acquisition (MS1)",
          "description": "MS1 scan range; precursors outside this m/z window are never transmitted.",
          "tier": "Ideal",
          "example": "350\u20131500"
        },
        {
          "id": "MS-16",
          "name": "ms1_agc_target",
          "subsection": "MS acquisition (MS1)",
          "description": "MS1 Automatic Gain Control target (drives detection efficiency).",
          "tier": "Ideal",
          "example": "1e6"
        },
        {
          "id": "MS-17",
          "name": "ms1_max_injection_time",
          "subsection": "MS acquisition (MS1)",
          "description": "MS1 maximum injection time.",
          "tier": "Ideal",
          "example": "50"
        },
        {
          "id": "MS-18",
          "name": "ms1_resolution",
          "subsection": "MS acquisition (MS1)",
          "description": "MS1 resolution.",
          "tier": "Ideal",
          "example": "60000"
        },
        {
          "id": "MS-19",
          "name": "min_intensity_threshold",
          "subsection": "Precursor gating",
          "description": "Minimum MS1 intensity threshold required to trigger fragmentation.",
          "tier": "Ideal",
          "example": "1e4"
        },
        {
          "id": "MS-20",
          "name": "cycle_time_peak_width",
          "subsection": "Precursor gating",
          "description": "Cycle-time vs peak-width relationship (number of sampling points across a peak).",
          "tier": "Ideal",
          "example": "12 s / 1.5 s"
        },
        {
          "id": "MS-21",
          "name": "topN",
          "subsection": "Precursor gating",
          "description": "Number of precursors fragmented per cycle (TopN).",
          "tier": "Ideal",
          "example": "12"
        },
        {
          "id": "MS-22",
          "name": "charge_state_range",
          "subsection": "Precursor gating",
          "description": "Allowed precursor charge states.",
          "tier": "Ideal",
          "example": "2+ to 6+"
        },
        {
          "id": "MS-23",
          "name": "isolation_window",
          "subsection": "Precursor gating",
          "description": "Isolation window width.",
          "tier": "Ideal",
          "example": "1.0"
        },
        {
          "id": "MS-24",
          "name": "ms2_fragmentation_method",
          "subsection": "MS2 fragmentation",
          "description": "Fragmentation method.",
          "tier": "Ideal",
          "example": "HCD"
        },
        {
          "id": "MS-25",
          "name": "normalized_collision_energy",
          "subsection": "MS2 fragmentation",
          "description": "Normalized collision energy (only for collision-based methods).",
          "tier": "Ideal",
          "example": "28"
        },
        {
          "id": "MS-26",
          "name": "ms2_resolution",
          "subsection": "MS2 fragmentation",
          "description": "MS2 resolution.",
          "tier": "Ideal",
          "example": "15000"
        },
        {
          "id": "MS-27",
          "name": "precursor_mass_tolerance",
          "subsection": "MS2 fragmentation",
          "description": "Mass accuracy / precursor tolerance.",
          "tier": "Ideal",
          "example": "5"
        },
        {
          "id": "MS-28",
          "name": "fragment_ion_coverage",
          "subsection": "MS2 fragmentation",
          "description": "Fragment ion coverage: matched vs theoretically possible fragment ions for the sequence.",
          "tier": "Ideal",
          "example": "8 / 10"
        },
        {
          "id": "MS-29",
          "name": "search_engine",
          "subsection": "Computational identification",
          "description": "Search engine identity and version.",
          "tier": "Minimum",
          "example": "MaxQuant 2.4"
        },
        {
          "id": "MS-30",
          "name": "fdr_threshold",
          "subsection": "Computational identification",
          "description": "FDR threshold and the level at which it is applied.",
          "tier": "Minimum",
          "example": "1% at PSM level"
        },
        {
          "id": "MS-31",
          "name": "decoy_strategy",
          "subsection": "Computational identification",
          "description": "Decoy strategy used for FDR control.",
          "tier": "Ideal",
          "example": "target-decoy (reversed)"
        },
        {
          "id": "MS-32",
          "name": "search_database",
          "subsection": "Computational identification",
          "description": "Search database (FASTA) source and version.",
          "tier": "Minimum",
          "example": "UniProt <organism>, 2024_03"
        },
        {
          "id": "MS-33",
          "name": "enzyme_specificity_setting",
          "subsection": "Computational identification",
          "description": "Enzyme-specificity setting used in the search.",
          "tier": "Ideal",
          "example": "semi-tryptic"
        },
        {
          "id": "MS-34",
          "name": "missed_cleavages",
          "subsection": "Computational identification",
          "description": "Allowed missed cleavages.",
          "tier": "Ideal",
          "example": "2"
        },
        {
          "id": "MS-35",
          "name": "modifications",
          "subsection": "Computational identification",
          "description": "Fixed/variable sequence modifications considered.",
          "tier": "Ideal",
          "example": "Met oxidation; deamidation"
        },
        {
          "id": "MS-36",
          "name": "peptide_length_range",
          "subsection": "Computational identification",
          "description": "Minimum/maximum peptide length searched.",
          "tier": "Ideal",
          "example": "2\u201350"
        },
        {
          "id": "MS-37",
          "name": "detection_call_aggregation",
          "subsection": "Computational identification",
          "description": "Rule for calling a peptide detected across replicate runs.",
          "tier": "Minimum",
          "example": "\u22652 of 3 replicates"
        },
        {
          "id": "MS-38",
          "name": "pride_deposit_id",
          "subsection": "Data deposition",
          "description": "Public repository accession for raw + processed data (e.g. PRIDE).",
          "tier": "Minimum",
          "example": "PXD0XXXXXX"
        },
        {
          "id": "MS-39",
          "name": "workflow_repository",
          "subsection": "Data deposition",
          "description": "Link to the reproducible end-to-end computational workflow (raw data \u2192 final peptide list).",
          "tier": "Ideal",
          "example": "repository URL"
        },
        {
          "id": "MS-40",
          "name": "peptide_sequence",
          "subsection": "Peptide-level output",
          "description": "Identified peptide sequence.",
          "tier": "Minimum",
          "example": "LVNELTEFAK"
        },
        {
          "id": "MS-41",
          "name": "parent_protein",
          "subsection": "Peptide-level output",
          "description": "Parent protein accession(s) for the peptide.",
          "tier": "Ideal",
          "example": "P00XXX"
        },
        {
          "id": "MS-42",
          "name": "ms1_intensity",
          "subsection": "Peptide-level output",
          "description": "MS1 intensity / flyability signal (abundance \u00d7 ionization \u00d7 transmission \u00d7 detection).",
          "tier": "Ideal",
          "example": "1.2e7"
        },
        {
          "id": "MS-43",
          "name": "detected_flag",
          "subsection": "Peptide-level output",
          "description": "Whether the peptide was classified as detected (the prediction target).",
          "tier": "Minimum",
          "example": "TRUE"
        }
      ]
    },
    {
      "sheet": "4. Bioactivity & CCM",
      "title": "STAGE 4 \u2014 Peptide bioactivity & cell-culture performance",
      "nFields": 14,
      "nMinimum": 9,
      "nIdeal": 5,
      "fields": [
        {
          "id": "BA-01",
          "name": "peptide_id",
          "subsection": "Peptide bioactivity",
          "description": "Foreign key to the identified peptide (sequence + run).",
          "tier": "Minimum",
          "example": "RUN_001:LVNELTEFAK"
        },
        {
          "id": "BA-02",
          "name": "known_bioactivity",
          "subsection": "Peptide bioactivity",
          "description": "Known bioactivity annotation(s) for the observed peptide.",
          "tier": "Minimum",
          "example": "ACE-inhibitory"
        },
        {
          "id": "BA-03",
          "name": "bioactivity_source_db",
          "subsection": "Peptide bioactivity",
          "description": "Source database for the known bioactivity annotation.",
          "tier": "Ideal",
          "example": "curated bioactive-peptide DB"
        },
        {
          "id": "BA-04",
          "name": "predicted_bioactivity",
          "subsection": "Peptide bioactivity",
          "description": "Predicted bioactivity (only from adequately validated tools, given current tool limitations).",
          "tier": "Ideal",
          "example": "\u2014"
        },
        {
          "id": "BA-05",
          "name": "prediction_tool",
          "subsection": "Peptide bioactivity",
          "description": "Tool and version used for bioactivity prediction.",
          "tier": "Ideal",
          "example": "tool + version"
        },
        {
          "id": "BA-06",
          "name": "ccm_formulation_id",
          "subsection": "CCM context",
          "description": "Unique identifier for the cell-culture-media (CCM) formulation tested.",
          "tier": "Minimum",
          "example": "CCM_001"
        },
        {
          "id": "BA-07",
          "name": "hydrolysate_id",
          "subsection": "CCM context",
          "description": "Foreign key to the hydrolysate incorporated into the media.",
          "tier": "Minimum",
          "example": "PH_001"
        },
        {
          "id": "BA-08",
          "name": "hydrolysate_inclusion",
          "subsection": "CCM context",
          "description": "Hydrolysate inclusion concentration in the media.",
          "tier": "Minimum",
          "example": "2"
        },
        {
          "id": "BA-09",
          "name": "media_components",
          "subsection": "CCM context",
          "description": "Full list of every media component and its concentration (mixtures behave differently from isolated peptides).",
          "tier": "Minimum",
          "example": "component table"
        },
        {
          "id": "BA-10",
          "name": "cell_line",
          "subsection": "CCM context",
          "description": "Cell line used in the evaluation.",
          "tier": "Minimum",
          "example": "<cell line>"
        },
        {
          "id": "BA-11",
          "name": "cell_line_context_data",
          "subsection": "CCM context",
          "description": "Generalizable cell-line-specific context data (e.g. RNA-seq profile).",
          "tier": "Ideal",
          "example": "dataset / accession"
        },
        {
          "id": "BA-12",
          "name": "performance_metric_type",
          "subsection": "Performance outcome",
          "description": "Type of quantitative performance metric measured (comparable across contexts).",
          "tier": "Minimum",
          "example": "proliferation"
        },
        {
          "id": "BA-13",
          "name": "performance_value",
          "subsection": "Performance outcome",
          "description": "Measured value of the performance metric.",
          "tier": "Minimum",
          "example": "1.8e6 cells/mL"
        },
        {
          "id": "BA-14",
          "name": "cost_per_litre",
          "subsection": "Performance outcome",
          "description": "Media cost per litre (cross-context economic performance metric).",
          "tier": "Ideal",
          "example": "12.5"
        }
      ]
    }
  ]
}