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Update app.py
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app.py
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@@ -1,7 +1,8 @@
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import gradio as gr
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import os
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os.environ["CUDA_VISIBLE_DEVICES"] = "-1" # Force CPU if needed
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import torch
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import numpy as np
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from PIL import Image
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from PIL import Image as PILImage
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@@ -13,31 +14,38 @@ from skimage.color import rgb2gray
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from csbdeep.utils import normalize
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from stardist.models import StarDist2D
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from stardist.plot import render_label
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from
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from
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from cellpose import models as cellpose_models, io as cellpose_io, plot as cellpose_plot
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# Load SegFormer
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from transformers import SegformerImageProcessor, SegformerForSemanticSegmentation
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processor_segformer = SegformerImageProcessor(do_reduce_labels=False)
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model_segformer = SegformerForSemanticSegmentation.from_pretrained(
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"nvidia/segformer-b0-finetuned-ade-512-512",
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num_labels=8,
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ignore_mismatched_sizes=True
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)
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model_segformer.load_state_dict(torch.load("trained_model_200.pt", map_location=
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model_segformer.eval()
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# StarDist model
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model_stardist = StarDist2D.from_pretrained('2D_versatile_fluo')
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# Cellpose model
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model_cellpose = cellpose_models.CellposeModel(gpu=
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#
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def infer_segformer(image):
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image = image.convert("RGB")
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inputs = processor_segformer(images=image, return_tensors="pt")
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with torch.no_grad():
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logits = model_segformer(**inputs).logits
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pred_mask = torch.argmax(logits, dim=1)[0].cpu().numpy()
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@@ -49,7 +57,7 @@ def infer_segformer(image):
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color_mask[pred_mask == c] = colors[c]
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return image, Image.fromarray(color_mask)
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#
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def infer_stardist(image):
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image_gray = rgb2gray(np.array(image)) if image.mode == 'RGB' else np.array(image)
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labels, _ = model_stardist.predict_instances(normalize(image_gray))
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@@ -57,12 +65,11 @@ def infer_stardist(image):
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overlay = (overlay[..., :3] * 255).astype(np.uint8)
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return image, Image.fromarray(overlay)
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#
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def infer_mediar(image, temp_dir="temp_mediar"):
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os.makedirs(temp_dir, exist_ok=True)
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input_path = os.path.join(temp_dir, "input_image.tiff")
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output_path = os.path.join(temp_dir, "input_image_label.tiff")
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image.save(input_path)
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model_args = {
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}
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model = MEDIARFormer(**model_args)
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weights = torch.load("from_phase1.pth", map_location=
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model.load_state_dict(weights, strict=False)
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model.eval()
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predictor = Predictor(model,
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predictor.img_names = ["input_image.tiff"]
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_ = predictor.conduct_prediction()
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buf.seek(0)
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return image, Image.open(buf)
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def infer_cellpose(image, temp_dir="temp_cellpose"):
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os.makedirs(temp_dir, exist_ok=True)
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input_path = os.path.join(temp_dir, "input_image.tif")
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output_overlay = os.path.join(temp_dir, "overlay.png")
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# Save image
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image.save(input_path)
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img = cellpose_io.imread(input_path)
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masks, flows, styles = model_cellpose.eval(img, batch_size=1)
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return image, Image.open(output_overlay)
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#
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def segment(model_name, image):
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ext = image.format.lower()
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if model_name == "Cellpose":
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# Accept only TIFF images for Cellpose
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if ext not in ["tiff", "tif", None]:
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return None, f"❌ Cellpose only supports `.tif` or `.tiff` images."
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# ...existing code...
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if model_name == "SegFormer":
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return infer_segformer(image)
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elif model_name == "StarDist":
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else:
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return None, f"❌ Unknown model: {model_name}"
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with gr.Blocks(title="Cell Segmentation Explorer") as app:
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gr.Markdown("## Cell Segmentation Explorer")
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gr.Markdown("Choose a segmentation model, upload an appropriate image, and view the predicted mask.")
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@@ -156,7 +159,7 @@ with gr.Blocks(title="Cell Segmentation Explorer") as app:
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def handle_submit(model_name, img):
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if img is None:
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return None
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_, result = segment(model_name, img)
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return result
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submit_btn.click(
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@@ -171,18 +174,12 @@ with gr.Blocks(title="Cell Segmentation Explorer") as app:
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outputs=[image_input, output_image]
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)
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# === SAMPLE IMAGES SECTION ===
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gr.Markdown("---")
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gr.Markdown("### Sample Images (click to use as input)")
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original_sample_paths = [
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"img1.png",
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"img2.png",
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"img3.png"
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]
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resized_sample_paths = []
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for idx, p in enumerate(original_sample_paths):
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img = PILImage.open(p).resize((128, 128))
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temp_path = f"/tmp/sample_resized_{idx}.png"
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@@ -192,7 +189,7 @@ with gr.Blocks(title="Cell Segmentation Explorer") as app:
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sample_image_components = []
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with gr.Row():
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for i, img_path in enumerate(resized_sample_paths):
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def load_full_image(idx=i):
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return PILImage.open(original_sample_paths[idx])
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sample_img = gr.Image(value=img_path, type="pil", interactive=True, show_label=False)
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)
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sample_image_components.append(sample_img)
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app.launch()
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import gradio as gr
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import os
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import torch
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import tensorflow as tf
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tf.config.set_visible_devices([], 'GPU')
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import numpy as np
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from PIL import Image
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from PIL import Image as PILImage
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from csbdeep.utils import normalize
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from stardist.models import StarDist2D
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from stardist.plot import render_label
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from train_tools.models import MEDIARFormer
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from core.MEDIAR import Predictor
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from cellpose import models as cellpose_models, io as cellpose_io, plot as cellpose_plot
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from transformers import SegformerImageProcessor, SegformerForSemanticSegmentation
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# === Setup for GPU or CPU ===
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device = torch.device("cuda" if torch.cuda.is_available() else "cpu")
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print(f"Using device: {device}")
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# Load SegFormer
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processor_segformer = SegformerImageProcessor(do_reduce_labels=False)
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model_segformer = SegformerForSemanticSegmentation.from_pretrained(
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"nvidia/segformer-b0-finetuned-ade-512-512",
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num_labels=8,
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ignore_mismatched_sizes=True
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)
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model_segformer.load_state_dict(torch.load("trained_model_200.pt", map_location=device))
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model_segformer.to(device)
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model_segformer.eval()
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# Load StarDist model (CPU-only, no GPU support)
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model_stardist = StarDist2D.from_pretrained('2D_versatile_fluo')
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# Load Cellpose model with GPU if available
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model_cellpose = cellpose_models.CellposeModel(gpu=torch.cuda.is_available())
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# SegFormer Inference
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def infer_segformer(image):
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image = image.convert("RGB")
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inputs = processor_segformer(images=image, return_tensors="pt")
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inputs = {k: v.to(device) for k, v in inputs.items()}
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with torch.no_grad():
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logits = model_segformer(**inputs).logits
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pred_mask = torch.argmax(logits, dim=1)[0].cpu().numpy()
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color_mask[pred_mask == c] = colors[c]
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return image, Image.fromarray(color_mask)
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# StarDist Inference
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def infer_stardist(image):
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image_gray = rgb2gray(np.array(image)) if image.mode == 'RGB' else np.array(image)
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labels, _ = model_stardist.predict_instances(normalize(image_gray))
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overlay = (overlay[..., :3] * 255).astype(np.uint8)
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return image, Image.fromarray(overlay)
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# MEDIAR Inference
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def infer_mediar(image, temp_dir="temp_mediar"):
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os.makedirs(temp_dir, exist_ok=True)
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input_path = os.path.join(temp_dir, "input_image.tiff")
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output_path = os.path.join(temp_dir, "input_image_label.tiff")
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image.save(input_path)
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model_args = {
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}
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model = MEDIARFormer(**model_args)
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weights = torch.load("MEDIAR_Weights/from_phase1.pth", map_location=device)
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model.load_state_dict(weights, strict=False)
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model.to(device)
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model.eval()
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predictor = Predictor(model, device.type, temp_dir, temp_dir, algo_params={"use_tta": False})
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predictor.img_names = ["input_image.tiff"]
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_ = predictor.conduct_prediction()
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buf.seek(0)
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return image, Image.open(buf)
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# Cellpose Inference
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def infer_cellpose(image, temp_dir="temp_cellpose"):
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os.makedirs(temp_dir, exist_ok=True)
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input_path = os.path.join(temp_dir, "input_image.tif")
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output_overlay = os.path.join(temp_dir, "overlay.png")
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image.save(input_path)
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img = cellpose_io.imread(input_path)
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masks, flows, styles = model_cellpose.eval(img, batch_size=1)
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return image, Image.open(output_overlay)
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# Main segmentation dispatcher
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def segment(model_name, image):
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ext = image.format.lower() if hasattr(image, 'format') and image.format else None
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if model_name == "Cellpose" and ext not in ["tif", "tiff", None]:
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return None, f"❌ Cellpose only supports `.tif` or `.tiff` images."
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if model_name == "SegFormer":
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return infer_segformer(image)
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elif model_name == "StarDist":
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else:
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return None, f"❌ Unknown model: {model_name}"
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# === Gradio UI ===
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with gr.Blocks(title="Cell Segmentation Explorer") as app:
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gr.Markdown("## Cell Segmentation Explorer")
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gr.Markdown("Choose a segmentation model, upload an appropriate image, and view the predicted mask.")
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def handle_submit(model_name, img):
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if img is None:
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return None
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_, result = segment(model_name, img)
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return result
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submit_btn.click(
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outputs=[image_input, output_image]
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)
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gr.Markdown("---")
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gr.Markdown("### Sample Images (click to use as input)")
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original_sample_paths = ["Sample Images/img1.png", "Sample Images/img2.png", "Sample Images/img3.png"]
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resized_sample_paths = []
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for idx, p in enumerate(original_sample_paths):
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img = PILImage.open(p).resize((128, 128))
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temp_path = f"/tmp/sample_resized_{idx}.png"
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sample_image_components = []
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with gr.Row():
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for i, img_path in enumerate(resized_sample_paths):
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def load_full_image(idx=i):
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return PILImage.open(original_sample_paths[idx])
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sample_img = gr.Image(value=img_path, type="pil", interactive=True, show_label=False)
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)
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sample_image_components.append(sample_img)
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app.launch()
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