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8ffc9f7 | 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 | {
"folder": "flash_seq",
"title": "FLASH-seq",
"crosscheck": {
"status": "checked",
"source_html_file": "SMART-seq_family.html",
"oligo_seq_recall": 0.118,
"oligo_seqs_matched": 2,
"oligo_seqs_total": 17,
"missed_oligos": [
"FLASH-seq_TSO",
"Fwd_PCR_primer",
"Illumina P5 adapter",
"Illumina P7 adapter",
"Nextera Index 1 sequencing primer (i7)",
"Nextera Index 2 sequencing primer (i5)",
"Nextera N7xx primer entry point (s7)",
"Nextera N/S5xx primer entry point (s5)",
"Nextera Tn5 binding site (19-bp Mosaic End (ME))",
"Nextera (XT) N7xx Index primer",
"Nextera (XT) N/S5xx Index primer",
"Rev_PCR_primer",
"Smartseq3_N8_TSO",
"Smartseq3_OligodT30VN",
"Smartseq3xpress_TSO"
],
"annotated_library_exact_match": false,
"annotated_library_got": "AATGATACGGCGACCACCGAGATCTACAC | [SAMPLE_INDEX:8](i5) | TCGTCGGCAGCGTC(ME-A handle / Read1 primer) | AGATGTGTATAAGAGACAG(mosaic end) | [CDNA] | CTGTCTCTTATACACATCT(rev mosaic end) | CCGAGCCCACGAGAC(ME-B handle, rev-comp) | [SAMPLE_INDEX:8](i7 rev-comp) | ATCTCGTATGCCGTCTTCTGCTTG",
"annotated_library_expected": "AATGATACGGCGACCACCGAGATCTACAC[I5_INDEX:8]TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATTGCGCAATG[UMI:8]CTAACGGG[CDNA]CTGTCTCTTATACACATCTCCGAGCCCACGAGAC[I7_INDEX:8]ATCTCGTATGCCGTCTTCTGCTTG",
"barcode_umi_length_diffs": {},
"big_conflict": true
}
}
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