Update app.py

app.py

@@ -393,7 +393,7 @@ with tab5:
     st.header("Decode Binary Labels to String")
 
     # Utility: Track source volumes and update if exceeds limit
-    def track_and_replace_source(source_list, robot_script, volume_limit=170):
+    def track_and_replace_source(source_list, robot_script, volume_limit=180):
         source_volumes = {}
         adjusted_sources = []
 
@@ -422,37 +422,33 @@ with tab5:
 
         return adjusted_sources, source_volumes
 
-    # Utility: Track D source volume separately for fixed-volume dispensing
-    def generate_fixed_d_source_instructions(df_robot, fixed_volume=16, volume_limit=170):
+    # Utility: Generate fixed-volume D source for all wells
+    def generate_fixed_d_source_instructions_for_all_wells(fixed_volume=16, volume_limit=170):
         d_source_volumes = {}
         d_source_script = []
         current_d_index = 1
 
-        mutation_headers = [str(h) for h in mutation_site_headers_actual_3614]
+        all_destinations = [f"{row}{col}" for row in "ABCDEFGH" for col in range(1, 13)]
+        for dest in all_destinations:
+            current_d_well = f"D{current_d_index}"
 
-        for row_idx, sample in df_robot.iterrows():
-            for col in mutation_headers:
-                if col in sample and int(sample[col]) == 1:
-                    current_d_well = f"D{current_d_index}"
-                    if current_d_well not in d_source_volumes:
-                        d_source_volumes[current_d_well] = 0
+            if current_d_well not in d_source_volumes:
+                d_source_volumes[current_d_well] = 0
 
-                    if d_source_volumes[current_d_well] + fixed_volume > volume_limit:
-                        current_d_index += 1
-                        current_d_well = f"D{current_d_index}"
-                        d_source_volumes[current_d_well] = 0
+            if d_source_volumes[current_d_well] + fixed_volume > volume_limit:
+                current_d_index += 1
+                current_d_well = f"D{current_d_index}"
+                d_source_volumes[current_d_well] = 0
 
-                    d_source_volumes[current_d_well] += fixed_volume
+            d_source_volumes[current_d_well] += fixed_volume
+            tool = 'TS_10' if fixed_volume < 10 else 'TS_50'
 
-                    dest = get_well_position(int(sample['Sample']))
-                    tool = 'TS_10' if fixed_volume < 10 else 'TS_50'
-
-                    d_source_script.append({
-                        'Source': current_d_well,
-                        'Destination': dest,
-                        'Volume': fixed_volume,
-                        'Tool': tool
-                    })
+            d_source_script.append({
+                'Source': current_d_well,
+                'Destination': dest,
+                'Volume': fixed_volume,
+                'Tool': tool
+            })
 
         return d_source_script, d_source_volumes
 
@@ -534,7 +530,7 @@ with tab5:
 
         robot_script_32, source_volumes_32 = track_and_replace_source(source_wells_32, robot_script_32)
 
-        d_script, d_volumes = generate_fixed_d_source_instructions(df_32_robot)
+        d_script, d_volumes = generate_fixed_d_source_instructions_for_all_wells()
         full_robot_script = robot_script_32 + d_script
 
         robot_script_32_df = pd.DataFrame(full_robot_script)