Update app.py

app.py

@@ -388,10 +388,43 @@ def get_well_position(sample_index):
     col_number = ((sample_index - 1) % 12) + 1
     return f"{row_letter}{col_number}"
 
+# Tab 5: Binary → String
 # Tab 5: Binary → String
 with tab5: 
     st.header("Decode Binary Labels to String")
 
+    # Utility: Track source volumes and update if exceeds limit
+    def track_and_replace_source(source_list, robot_script, volume_limit=180):
+        source_volumes = {}
+        adjusted_sources = []
+
+        for entry in robot_script:
+            src = entry['Source']
+            vol = entry['Volume']
+
+            if src not in source_volumes:
+                source_volumes[src] = 0
+
+            source_volumes[src] += vol
+
+            if source_volumes[src] > volume_limit:
+                # Convert A->E, B->F, ..., by shifting ASCII code +4
+                row_letter = src[0]
+                col_number = src[1:]
+                new_row_letter = chr(ord(row_letter) + 4)
+                new_src = f"{new_row_letter}{col_number}"
+                entry['Source'] = new_src
+
+                # Reset volume tracking for new source
+                if new_src not in source_volumes:
+                    source_volumes[new_src] = 0
+                source_volumes[new_src] += vol
+                source_volumes[src] -= vol
+
+            adjusted_sources.append(entry)
+
+        return adjusted_sources, source_volumes
+
     # ========== 32-BIT DECODING ==========
     st.subheader("32-bit Binary per Row")
     st.write("Upload CSV with 32 columns (0 or 1), no headers, from EF Binary format or enter manually below.")
@@ -453,10 +486,19 @@ with tab5:
                     vol = round(sample['volume donors (µl)'], 2)
                     robot_script_32.append({'Source': source, 'Destination': dest, 'Volume': vol})
 
+        # Adjust for source well volume limit
+        robot_script_32, source_volumes_32 = track_and_replace_source(source_wells_32, robot_script_32)
+
         robot_script_32_df = pd.DataFrame(robot_script_32)
         st.dataframe(robot_script_32_df)
         st.download_button("Download Robot Script (32-bit)", robot_script_32_df.to_csv(index=False), "robot_script_32bit.csv", key="download_robot_32")
 
+        # Show total volume per source well
+        st.subheader("Total Volume Used Per Source")
+        source_volume_df = pd.DataFrame(list(source_volumes_32.items()), columns=['Source', 'Total Volume (µl)'])
+        st.dataframe(source_volume_df)
+        st.download_button("Download Source Volumes", source_volume_df.to_csv(index=False), "source_total_volumes.csv", key="download_volume_32")
+
     st.markdown("---")
 
     # ========== 31-BIT DECODING ==========