Update app.py

app.py

@@ -422,13 +422,14 @@ with tab5:
 
         return adjusted_sources, source_volumes
 
-    # Utility: Generate fixed-volume D source for only used wells
-    def generate_fixed_d_source_instructions_for_used_wells(destinations, fixed_volume=16, volume_limit=170):
+    # Utility: Generate fixed-volume D source to all sample wells
+    def generate_fixed_d_source_instructions_to_all_samples(n_samples, fixed_volume=16, volume_limit=170):
         d_source_volumes = {}
         d_source_script = []
         current_d_index = 1
 
-        for dest in destinations:
+        for i in range(n_samples):
+            dest = get_well_position(i + 1)
             current_d_well = f"D{current_d_index}"
 
             if current_d_well not in d_source_volumes:
@@ -531,7 +532,7 @@ with tab5:
 
         robot_script_32, source_volumes_32 = track_and_replace_source(source_wells_32, robot_script_32)
 
-        d_script, d_volumes = generate_fixed_d_source_instructions_for_used_wells(sorted(used_destinations))
+        d_script, d_volumes = generate_fixed_d_source_instructions_to_all_samples(len(df_32_robot))
         full_robot_script = robot_script_32 + d_script
 
         robot_script_32_df = pd.DataFrame(full_robot_script)