updated generate_fixed_d_source_instructions

app.py

@@ -424,38 +424,43 @@ with tab5:
         return adjusted_sources, source_volumes
 
     # Utility: Track D source volume separately for fixed-volume dispensing
+    # Updateed
     def generate_fixed_d_source_instructions(df_robot, fixed_volume=16, volume_limit=170):
         d_source_volumes = {}
         d_source_script = []
         current_d_index = 1
-
+    
+        # Only use the actual 32 site mutation headers
+        mutation_headers = [str(h) for h in mutation_site_headers_actual_3614]
+    
         for row_idx, sample in df_robot.iterrows():
-            for col in df_robot.columns[1:]:
-                if int(sample[col]) == 1:
-                    # Check current D well volume
+            for col in mutation_headers:
+                if col in sample and int(sample[col]) == 1:
                     current_d_well = f"D{current_d_index}"
+    
                     if current_d_well not in d_source_volumes:
                         d_source_volumes[current_d_well] = 0
-
+    
                     if d_source_volumes[current_d_well] + fixed_volume > volume_limit:
                         current_d_index += 1
                         current_d_well = f"D{current_d_index}"
                         d_source_volumes[current_d_well] = 0
-
+    
                     d_source_volumes[current_d_well] += fixed_volume
-
+    
                     dest = get_well_position(int(sample['Sample']))
                     tool = 'TS_10' if fixed_volume < 10 else 'TS_50'
-
+    
                     d_source_script.append({
                         'Source': current_d_well,
                         'Destination': dest,
                         'Volume': fixed_volume,
                         'Tool': tool
                     })
-
+    
         return d_source_script, d_source_volumes
 
+
     # ========== 32-BIT DECODING ==========
     st.subheader("32-bit Binary per Row")
     st.write("Upload CSV with 32 columns (0 or 1), no headers, from EF Binary format or enter manually below.")