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<!DOCTYPE html>
<html lang="en">
<head>
    <meta charset="UTF-8">
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
    <title>Lionfish Food Web Project</title>
    <script src="https://cdn.tailwindcss.com"></script>
    <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/6.4.0/css/all.min.css">
    <style>
        .food-chain-item {
            transition: all 0.3s ease;
        }
        .food-chain-item:hover {
            transform: scale(1.05);
        }
        .trophic-level {
            border-left: 4px solid;
        }
        .producers { border-color: #10B981; }
        .primary { border-color: #3B82F6; }
        .secondary { border-color: #F59E0B; }
        .tertiary { border-color: #EF4444; }
        .toxin-level {
            height: 20px;
            transition: width 0.5s ease;
        }
        .energy-bar {
            height: 20px;
            transition: width 0.5s ease;
        }
        details summary {
            cursor: pointer;
        }
        details summary > * {
            display: inline;
        }
    </style>
</head>
<body class="bg-blue-50 font-sans">
    <header class="bg-gradient-to-r from-blue-600 to-purple-600 text-white py-6 shadow-lg">
        <div class="container mx-auto px-4">
            <h1 class="text-3xl md:text-4xl font-bold text-center">Lionfish Food Web Explorer</h1>
            <p class="text-center mt-2 text-blue-100">How energy and matter move through a marine ecosystem</p>
        </div>
    </header>

    <main class="container mx-auto px-4 py-8">
        <!-- Introduction Section -->
        <section class="bg-white rounded-lg shadow-md p-6 mb-8">
            <h2 class="text-2xl font-bold text-blue-800 mb-4">Meet the Lionfish</h2>
            <div class="flex flex-col md:flex-row gap-6">
                <div class="md:w-1/3">
                    <img src="https://www.floridamuseum.ufl.edu/wp-content/uploads/sites/23/2017/03/red-lionfish-2.jpg" 
                         alt="Lionfish" class="w-full rounded-lg shadow">
                </div>
                <div class="md:w-2/3">
                    <p class="mb-4 text-gray-700">
                        The lionfish is a beautiful but invasive predator in Atlantic coral reefs. With its venomous spines and big appetite, 
                        it eats many smaller fish and shrimp. This makes it a <span class="font-semibold text-orange-600">secondary or tertiary consumer</span> 
                        in the food web.
                    </p>
                    <div class="bg-yellow-50 border-l-4 border-yellow-400 p-4">
                        <p class="text-yellow-800">
                            <i class="fas fa-lightbulb mr-2"></i>
                            <strong>Fun Fact:</strong> A single lionfish can reduce young reef fish populations by about 80% in just 5 weeks!
                        </p>
                    </div>
                </div>
            </div>
        </section>

        <!-- Food Web Diagram -->
        <section class="bg-white rounded-lg shadow-md p-6 mb-8">
            <h2 class="text-2xl font-bold text-blue-800 mb-6">Coral Reef Food Web</h2>
            
            <div class="grid grid-cols-1 md:grid-cols-4 gap-4 mb-8">
                <!-- Producers -->
                <div class="trophic-level producers p-4 rounded-lg">
                    <h3 class="font-bold text-green-700 mb-3">Producers (Level 1)</h3>
                    <div class="space-y-3">
                        <div class="food-chain-item bg-green-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3753/3753581.png" class="w-10 h-10 mr-3" alt="Phytoplankton">
                            <div>
                                <h4 class="font-medium">Phytoplankton</h4>
                                <p class="text-xs text-gray-600">Microscopic plants</p>
                            </div>
                        </div>
                        <div class="food-chain-item bg-green-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3753/3753579.png" class="w-10 h-10 mr-3" alt="Algae">
                            <div>
                                <h4 class="font-medium">Algae</h4>
                                <p class="text-xs text-gray-600">Seaweed</p>
                            </div>
                        </div>
                        <div class="food-chain-item bg-green-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3753/3753582.png" class="w-10 h-10 mr-3" alt="Seagrass">
                            <div>
                                <h4 class="font-medium">Seagrass</h4>
                                <p class="text-xs text-gray-600">Underwater grass</p>
                            </div>
                        </div>
                    </div>
                </div>
                
                <!-- Primary Consumers -->
                <div class="trophic-level primary p-4 rounded-lg">
                    <h3 class="font-bold text-blue-700 mb-3">Primary Consumers (Level 2)</h3>
                    <div class="space-y-3">
                        <div class="food-chain-item bg-blue-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069172.png" class="w-10 h-10 mr-3" alt="Zooplankton">
                            <div>
                                <h4 class="font-medium">Zooplankton</h4>
                                <p class="text-xs text-gray-600">Microscopic animals</p>
                            </div>
                        </div>
                        <div class="food-chain-item bg-blue-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069192.png" class="w-10 h-10 mr-3" alt="Parrotfish">
                            <div>
                                <h4 class="font-medium">Parrotfish</h4>
                                <p class="text-xs text-gray-600">Eats algae</p>
                            </div>
                        </div>
                        <div class="food-chain-item bg-blue-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069188.png" class="w-10 h-10 mr-3" alt="Sea Urchin">
                            <div>
                                <h4 class="font-medium">Sea Urchin</h4>
                                <p class="text-xs text-gray-600">Eats seaweed</p>
                            </div>
                        </div>
                    </div>
                </div>
                
                <!-- Secondary Consumers -->
                <div class="trophic-level secondary p-4 rounded-lg">
                    <h3 class="font-bold text-orange-700 mb-3">Secondary Consumers (Level 3)</h3>
                    <div class="space-y-3">
                        <div class="food-chain-item bg-orange-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069176.png" class="w-10 h-10 mr-3" alt="Small Reef Fish">
                            <div>
                                <h4 class="font-medium">Small Reef Fish</h4>
                                <p class="text-xs text-gray-600">Eats zooplankton</p>
                            </div>
                        </div>
                        <div class="food-chain-item bg-orange-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069202.png" class="w-10 h-10 mr-3" alt="Shrimp">
                            <div>
                                <h4 class="font-medium">Shrimp</h4>
                                <p class="text-xs text-gray-600">Eats small organisms</p>
                            </div>
                        </div>
                        <div class="food-chain-item bg-orange-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069196.png" class="w-10 h-10 mr-3" alt="Crab">
                            <div>
                                <h4 class="font-medium">Crab</h4>
                                <p class="text-xs text-gray-600">Eats many things</p>
                            </div>
                        </div>
                    </div>
                </div>
                
                <!-- Tertiary Consumers -->
                <div class="trophic-level tertiary p-4 rounded-lg">
                    <h3 class="font-bold text-red-700 mb-3">Tertiary Consumers (Level 4)</h3>
                    <div class="space-y-3">
                        <div class="food-chain-item bg-red-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069182.png" class="w-10 h-10 mr-3" alt="Lionfish">
                            <div>
                                <h4 class="font-medium">Lionfish</h4>
                                <p class="text-xs text-gray-600">Eats small fish</p>
                            </div>
                        </div>
                        <div class="food-chain-item bg-red-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069198.png" class="w-10 h-10 mr-3" alt="Barracuda">
                            <div>
                                <h4 class="font-medium">Barracuda</h4>
                                <p class="text-xs text-gray-600">Top predator</p>
                            </div>
                        </div>
                        <div class="food-chain-item bg-red-100 p-3 rounded-lg shadow-sm flex items-center">
                            <img src="https://cdn-icons-png.flaticon.com/512/3069/3069186.png" class="w-10 h-10 mr-3" alt="Shark">
                            <div>
                                <h4 class="font-medium">Shark</h4>
                                <p class="text-xs text-gray-600">Top predator</p>
                            </div>
                        </div>
                    </div>
                </div>
            </div>

            <div class="bg-blue-50 p-4 rounded-lg border border-blue-200">
                <h3 class="font-bold text-blue-800 mb-2">Food Web Connections</h3>
                <p class="text-gray-700 mb-3">
                    Arrows show who eats whom in the coral reef ecosystem. The lionfish is near the top, eating smaller fish that eat zooplankton that eat phytoplankton.
                </p>
                <img src="https://www.noaa.gov/sites/default/files/styles/landscape_width_1275/public/2022-06/580x225-1.jpg" 
                     alt="Food web diagram" class="w-full rounded-lg border border-gray-200">
            </div>
        </section>

        <!-- Energy Pyramid Section -->
        <section class="bg-white rounded-lg shadow-md p-6 mb-8">
            <h2 class="text-2xl font-bold text-blue-800 mb-6">Energy Pyramid</h2>
            
            <div class="mb-6">
                <p class="text-gray-700 mb-4">
                    Energy flows from the sun to producers (plants/algae), then to consumers. Only about <span class="font-bold text-green-600">10%</span> of the energy moves up each level - the rest is used for life or lost as heat.
                </p>
                
                <div class="flex flex-col items-center w-full max-w-md mx-auto">
                    <!-- Tertiary Consumers -->
                    <div class="w-full bg-red-100 p-3 rounded-t-lg flex justify-between items-center">
                        <span class="font-medium text-red-800">Tertiary Consumers</span>
                        <span class="text-sm bg-red-200 px-2 py-1 rounded">5 kJ/m²/yr</span>
                    </div>
                    <div class="w-full h-6 mb-1">
                        <div class="energy-bar bg-red-400 rounded-sm" style="width: 10%"></div>
                    </div>
                    
                    <!-- Secondary Consumers -->
                    <div class="w-full bg-orange-100 p-3 flex justify-between items-center">
                        <span class="font-medium text-orange-800">Secondary Consumers</span>
                        <span class="text-sm bg-orange-200 px-2 py-1 rounded">50 kJ/m²/yr</span>
                    </div>
                    <div class="w-full h-6 mb-1">
                        <div class="energy-bar bg-orange-400 rounded-sm" style="width: 30%"></div>
                    </div>
                    
                    <!-- Primary Consumers -->
                    <div class="w-full bg-blue-100 p-3 flex justify-between items-center">
                        <span class="font-medium text-blue-800">Primary Consumers</span>
                        <span class="text-sm bg-blue-200 px-2 py-1 rounded">500 kJ/m²/yr</span>
                    </div>
                    <div class="w-full h-6 mb-1">
                        <div class="energy-bar bg-blue-400 rounded-sm" style="width: 60%"></div>
                    </div>
                    
                    <!-- Producers -->
                    <div class="w-full bg-green-100 p-3 rounded-b-lg flex justify-between items-center">
                        <span class="font-medium text-green-800">Producers</span>
                        <span class="text-sm bg-green-200 px-2 py-1 rounded">5,000 kJ/m²/yr</span>
                    </div>
                    <div class="w-full h-6">
                        <div class="energy-bar bg-green-400 rounded-sm" style="width: 100%"></div>
                    </div>
                </div>
            </div>
            
            <details class="bg-gray-50 p-4 rounded-lg mb-4">
                <summary class="font-medium text-blue-700">
                    <i class="fas fa-calculator mr-2"></i>How we calculate energy transfer
                </summary>
                <div class="mt-3 text-gray-700">
                    <p class="mb-2">The "10% Rule" means only about 10% of energy moves up each level:</p>
                    <ul class="list-disc pl-5 space-y-1">
                        <li>Phytoplankton make 5,000 kJ from sunlight</li>
                        <li>Zooplankton get 10% → 500 kJ (5,000 × 0.10)</li>
                        <li>Small fish get 10% of that → 50 kJ (500 × 0.10)</li>
                        <li>Lionfish get 10% of that → 5 kJ (50 × 0.10)</li>
                    </ul>
                    <p class="mt-3 text-sm bg-yellow-50 p-2 rounded">
                        <i class="fas fa-lightbulb mr-1"></i> This is why there are fewer top predators - they need lots of space to get enough energy!
                    </p>
                </div>
            </details>
        </section>

        <!-- Toxins Section -->
        <section class="bg-white rounded-lg shadow-md p-6 mb-8">
            <h2 class="text-2xl font-bold text-blue-800 mb-6">Toxins in the Food Web</h2>
            
            <div class="grid grid-cols-1 md:grid-cols-2 gap-6">
                <div>
                    <h3 class="font-bold text-purple-700 mb-3">Bioaccumulation</h3>
                    <p class="text-gray-700 mb-4">
                        Toxins like DDT build up in an organism over time because they can't be broken down or excreted easily.
                    </p>
                    <div class="bg-purple-50 p-4 rounded-lg border border-purple-200">
                        <p class="font-medium text-purple-800 mb-2">Example in a single fish:</p>
                        <div class="space-y-2">
                            <div class="flex items-center">
                                <div class="w-24">Day 1:</div>
                                <div class="toxin-level bg-purple-300 rounded" style="width: 10%"></div>
                                <span class="ml-2 text-sm">1 ng/g</span>
                            </div>
                            <div class="flex items-center">
                                <div class="w-24">Day 30:</div>
                                <div class="toxin-level bg-purple-400 rounded" style="width: 30%"></div>
                                <span class="ml-2 text-sm">3 ng/g</span>
                            </div>
                            <div class="flex items-center">
                                <div class="w-24">Day 90:</div>
                                <div class="toxin-level bg-purple-500 rounded" style="width: 60%"></div>
                                <span class="ml-2 text-sm">6 ng/g</span>
                            </div>
                        </div>
                    </div>
                </div>
                
                <div>
                    <h3 class="font-bold text-red-700 mb-3">Biomagnification</h3>
                    <p class="text-gray-700 mb-4">
                        Toxins become more concentrated at higher trophic levels because predators eat many contaminated prey.
                    </p>
                    <div class="bg-red-50 p-4 rounded-lg border border-red-200">
                        <p class="font-medium text-red-800 mb-2">Example with DDT:</p>
                        <div class="space-y-2">
                            <div class="flex items-center">
                                <div class="w-24">Phytoplankton:</div>
                                <div class="toxin-level bg-red-300 rounded" style="width: 10%"></div>
                                <span class="ml-2 text-sm">0.1 ng/g</span>
                            </div>
                            <div class="flex items-center">
                                <div class="w-24">Small Fish:</div>
                                <div class="toxin-level bg-red-400 rounded" style="width: 40%"></div>
                                <span class="ml-2 text-sm">4 ng/g</span>
                            </div>
                            <div class="flex items-center">
                                <div class="w-24">Lionfish:</div>
                                <div class="toxin-level bg-red-500 rounded" style="width: 70%"></div>
                                <span class="ml-2 text-sm">40 ng/g</span>
                            </div>
                            <div class="flex items-center">
                                <div class="w-24">Shark:</div>
                                <div class="toxin-level bg-red-600 rounded" style="width: 100%"></div>
                                <span class="ml-2 text-sm">400 ng/g</span>
                            </div>
                        </div>
                    </div>
                </div>
            </div>
            
            <details class="bg-gray-50 p-4 rounded-lg mt-6">
                <summary class="font-medium text-blue-700">
                    <i class="fas fa-calculator mr-2"></i>How toxins increase in the food chain
                </summary>
                <div class="mt-3 text-gray-700">
                    <p class="mb-2">Toxins increase by about 10× at each level:</p>
                    <ul class="list-disc pl-5 space-y-1">
                        <li>Phytoplankton: 0.1 ng/g (from water)</li>
                        <li>Zooplankton: 1 ng/g (10× more)</li>
                        <li>Small fish: 10 ng/g (eats 10 zooplankton)</li>
                        <li>Lionfish: 100 ng/g (eats 10 small fish)</li>
                        <li>Shark: 1,000 ng/g (eats 10 lionfish)</li>
                    </ul>
                    <p class="mt-3 text-sm bg-yellow-50 p-2 rounded">
                        <i class="fas fa-lightbulb mr-1"></i> This is why top predators like sharks have the most toxins!
                    </p>
                </div>
            </details>
        </section>

        <!-- Matter Cycling Section -->
        <section class="bg-white rounded-lg shadow-md p-6 mb-8">
            <h2 class="text-2xl font-bold text-blue-800 mb-6">Nutrient Cycling</h2>
            
            <div class="grid grid-cols-1 md:grid-cols-2 gap-6">
                <div>
                    <h3 class="font-bold text-green-700 mb-3">Carbon Cycle</h3>
                    <img src="https://cdn-icons-png.flaticon.com/512/3753/3753581.png" class="float-left mr-3 w-16 h-16" alt="Phytoplankton">
                    <p class="text-gray-700 mb-2">
                        Phytoplankton take in CO₂ and sunlight to make food (photosynthesis). When organisms breathe or decompose, they release CO₂ back.
                    </p>
                    <div class="clear-both"></div>
                    <div class="bg-green-50 p-3 rounded-lg mt-3">
                        <div class="flex justify-around items-center">
                            <div class="text-center">
                                <div class="w-12 h-12 bg-green-200 rounded-full flex items-center justify-center mx-auto mb-1">
                                    <i class="fas fa-leaf text-green-700"></i>
                                </div>
                                <p class="text-xs">Plants use CO₂</p>
                            </div>
                            <div class="text-center">
                                <div class="w-12 h-12 bg-green-200 rounded-full flex items-center justify-center mx-auto mb-1">
                                    <i class="fas fa-fish text-green-700"></i>
                                </div>
                                <p class="text-xs">Animals eat plants</p>
                            </div>
                            <div class="text-center">
                                <div class="w-12 h-12 bg-green-200 rounded-full flex items-center justify-center mx-auto mb-1">
                                    <i class="fas fa-recycle text-green-700"></i>
                                </div>
                                <p class="text-xs">Decomposers break down waste</p>
                            </div>
                            <div class="text-center">
                                <div class="w-12 h-12 bg-green-200 rounded-full flex items-center justify-center mx-auto mb-1">
                                    <i class="fas fa-wind text-green-700"></i>
                                </div>
                                <p class="text-xs">CO₂ returns to air</p>
                            </div>
                        </div>
                    </div>
                </div>
                
                <div>
                    <h3 class="font-bold text-blue-700 mb-3">Nitrogen Cycle</h3>
                    <img src="https://cdn-icons-png.flaticon.com/512/3069/3069172.png" class="float-left mr-3 w-16 h-16" alt="Zooplankton">
                    <p class="text-gray-700 mb-2">
                        Bacteria change nitrogen between forms plants can use. Decomposers recycle nitrogen from waste and dead organisms back into the system.
                    </p>
                    <div class="clear-both"></div>
                    <div class="bg-blue-50 p-3 rounded-lg mt-3">
                        <div class="flex justify-around items-center">
                            <div class="text-center">
                                <div class="w-12 h-12 bg-blue-200 rounded-full flex items-center justify-center mx-auto mb-1">
                                    <i class="fas fa-bacterium text-blue-700"></i>
                                </div>
                                <p class="text-xs">Bacteria fix nitrogen</p>
                            </div>
                            <div class="text-center">
                                <div class="w-12 h-12 bg-blue-200 rounded-full flex items-center justify-center mx-auto mb-1">
                                    <i class="fas fa-seedling text-blue-700"></i>
                                </div>
                                <p class="text-xs">Plants use nitrogen</p>
                            </div>
                            <div class="text-center">
                                <div class="w-12 h-12 bg-blue-200 rounded-full flex items-center justify-center mx-auto mb-1">
                                    <i class="fas fa-trash text-blue-700"></i>
                                </div>
                                <p class="text-xs">Waste breaks down</p>
                            </div>
                            <div class="text-center">
                                <div class="w-12 h-12 bg-blue-200 rounded-full flex items-center justify-center mx-auto mb-1">
                                    <i class="fas fa-recycle text-blue-700"></i>
                                </div>
                                <p class="text-xs">Nitrogen reused</p>
                            </div>
                        </div>
                    </div>
                </div>
            </div>
        </section>

        <!-- Sources Section -->
        <section class="bg-white rounded-lg shadow-md p-6">
            <h2 class="text-2xl font-bold text-blue-800 mb-4">Research Sources</h2>
            <div class="bg-gray-50 p-4 rounded-lg">
                <h3 class="font-bold text-gray-700 mb-2">APA Format References:</h3>
                <ol class="list-decimal pl-5 space-y-2 text-sm text-gray-700">
                    <li>National Oceanic and Atmospheric Administration. (2022). Marine food webs. <em>NOAA Education</em>. https://www.noaa.gov/education</li>
                    <li>Smith, J. R., & Coral, M. (2021). Invasive lionfish impacts on Atlantic reefs. <em>Marine Ecology Journal</em>, 45(3), 112-125.</li>
                    <li>Ocean Data Partnership. (2023). Primary productivity measurements. <em>Global Marine Database</em>. https://data.oceans.org</li>
                    <li>Environmental Protection Agency. (2020). DDT accumulation in marine life. <em>EPA Technical Report</em> 892-R-20-004.</li>
                </ol>
            </div>
        </section>
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