Update app.py

app.py

@@ -60,11 +60,14 @@ if uploaded_file is not None:
 
     product_size_range = st.text_input("Enter desired PCR product size range (e.g., 150-500):", value="150-500")
 
+    # Let users specify the minimum number of primer pairs to return, ensuring it's at least 5
+    min_num_primers = st.number_input("Enter minimum number of primer pairs to return:", min_value=5, value=5, step=1)
+
     if st.button(f'Design Primers for selected {feature_type}'):
-        primers = design_primers_for_region(feature_sequence, product_size_range, num_to_return=5)
+        primers = design_primers_for_region(feature_sequence, product_size_range, num_to_return=min_num_primers)
         
         primer_data = []
-        for i in range(5):  # Collect data for 5 primer pairs
+        for i in range(min_num_primers):  # Collect data for the specified number of primer pairs
             left_sequence = primers.get(f'PRIMER_LEFT_{i}_SEQUENCE', 'N/A')
             right_sequence = primers.get(f'PRIMER_RIGHT_{i}_SEQUENCE', 'N/A')
             if left_sequence != 'N/A' and right_sequence != 'N/A':