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Update app.py
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app.py
CHANGED
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@@ -109,7 +109,21 @@ if uploaded_file is not None:
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'PCR Product Size (bp)': primers.get(f'PRIMER_PAIR_{i}_PRODUCT_SIZE', 'N/A')
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}
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if primer_info['Left Sequence'] != 'N/A' and primer_info['Right Sequence'] != 'N/A':
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primer_data.append(primer_info)
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if primer_data:
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primer_df = pd.DataFrame(primer_data)
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@@ -129,4 +143,4 @@ if uploaded_file is not None:
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feature_length = len(feature_sequence) # Obtain the length of the selected feature
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plot_pcr_product_with_df(feature_sequence, primer_df, feature_length)
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else:
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st.error('No primers were found. Please adjust your parameters and try again.')
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'PCR Product Size (bp)': primers.get(f'PRIMER_PAIR_{i}_PRODUCT_SIZE', 'N/A')
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}
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if primer_info['Left Sequence'] != 'N/A' and primer_info['Right Sequence'] != 'N/A':
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primer_info['Primer'] = f"Left {i+1}"
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primer_data.append(primer_info)
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primer_info = {
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'Primer Pair': i + 1,
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'Left Sequence': primers.get(f'PRIMER_LEFT_{i}_SEQUENCE', 'N/A'),
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'Right Sequence': primers.get(f'PRIMER_RIGHT_{i}_SEQUENCE', 'N/A'),
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'Left TM (°C)': primers.get(f'PRIMER_LEFT_{i}_TM', 'N/A'),
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'Right TM (°C)': primers.get(f'PRIMER_RIGHT_{i}_TM', 'N/A'),
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'Left Length': primers.get(f'PRIMER_LEFT_{i}_SIZE', 'N/A'),
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'Right Length': primers.get(f'PRIMER_RIGHT_{i}_SIZE', 'N/A'),
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'PCR Product Size (bp)': primers.get(f'PRIMER_PAIR_{i}_PRODUCT_SIZE', 'N/A')
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}
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if primer_info['Left Sequence'] != 'N/A' and primer_info['Right Sequence'] != 'N/A':
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primer_info['Primer'] = f"Right {i+1}"
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primer_data.append(primer_info)
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if primer_data:
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primer_df = pd.DataFrame(primer_data)
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feature_length = len(feature_sequence) # Obtain the length of the selected feature
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plot_pcr_product_with_df(feature_sequence, primer_df, feature_length)
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else:
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st.error('No primers were found. Please adjust your parameters and try again.')
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