text stringlengths 1 27.2k | document_url stringclasses 590
values | source_url stringclasses 590
values | country stringclasses 7
values |
|---|---|---|---|
We analyzed how mosquitoes’ resistance levels affect their response to synergist exposure.
This review collected 2,799 paired (insecticide and paired synergized insecticides) records covering six major types of synergists and 26 mosquito species (complexes) from four genera.
Piperonyl butoxide (PBO) was the most test... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
PBO pre-exposure nearly fully restored the susceptibility of Anopheles funestus and Anopheles arabiensis to pyrethroid insecticides, but not with highly insecticide-resistant Anopheles gambiae sensu lato, Culex, and Aedes mosquitoes.
PBO was also a good synergist for DDT and carbamate insecticides.
S, S, S-tributyl p... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
PBO-treated long-lasting insecticidal nets (PBO-LLINs) significantly increased mosquito mortality compared to regular LLINs; however, their effect was compromised against highly insecticide-resistant mosquitoes.
There was not enough evidence to examine how synergist exposure affects metabolic enzymatic activity in mos... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Potential development of mosquito tolerance to synergized insecticide products needs to be closely monitored.1314QUANTIFICATIONS OF CYTOCHROME P450S GENES INVOLVE IN ANOPHELES GAMBIAE S.L. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
METABOLIC RESISTANCE IN THE CASCADES REGION OF BURKINA FASOAntoine Sanou1, Moussa W. Guelbeogo1, Madou Tapsoba1, Soumanaba Zongo1, Fatoumata Cisse1, Benoit Sanon1, Nfale Sagnon1, Geraldine M. Foster2, Hilary Ranson21Centre National de Recherche et de Formation sur le Paludisme, Ouagagougou, Burkina Faso, 2Liverpool Sch... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
With the development of resistance to the recommended classes of insecticides the efficacy of these tools is threatened.
To mitigate this resistance, Burkina Faso, one of the ten-plus-one high malaria burden countries have successfully piloted new generation nets (NGN) including Piperonyl Butoxide (PBO)-pyrethroid and... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The current study assessed the variation in expression levels of known cytochrome P450s genes associated with metabolic insecticide resistance on data collected between 2020 and 2022.
Mosquito populations were assessed using adults reared from larvae collected from different aquatic habitats from three villages with h... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Collections began in 2020 and ended in 2022.
Mosquitoes were exposed to deltamethrin and alphacypermethrin after pre-exposure to PBO following WHO tube bioassay guidelines then stored in RNAlater.
cDNA developed from extracted mRNA will be use during a multiplex TaqMan qPCR to assess for the variation in expression o... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The overall mean of mortality rate was < 20% after exposure to the diagnostic dose and varied between sites and insecticide used.
The pre-exposure to PBO improved the mortality rate to between 79 and 86%, depending on collection site and insecticide.
These results indicate an involvement of cytochrome P450 and estera... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The multiplex qPCR results will inform on the presence of the given metabolic resistance genes and the variation in their expression level over years.1315415NEXT GENERATION MOSQUITO CONTROL: REMOTE SENSING AND APPLIED MACHINE LEARNING TECHNIQUES ACCURATELY PREDICT CULEX SP. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
ABUNDANCE AND MOSQUITO BREEDING HABITATSSarah Gunter1, Abiodun Oluyomi1, Timothy Erickson1, Huixuan Li2, Chris Fredregill3, Jerry Helfand1, Melissa Nolan21Baylor College of Medicine, Houston, TX, United States, 2University of South Carolina, Columbia, SC, United States, 3Harris County Mosquito and Vector Control Divisi... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
With few effective therapeutics or preventative vaccine available, public health officials rely on targeted mosquito abatement efforts to reduce the burden of disease.
Effective insecticides have been developed to reduce the mosquito population and therefore reduce disease transmission.
However, widespread insecticid... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Targeted mosquito abatement efforts are critical to effectively reducing the burden of disease in affected areas.
Our current collaborative project is proof-of-concept study demonstrating the use of remote sensing and machine learning to effectively predict Culex sp.
mosquito abundance in Harris County, Texas.
Mosqu... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Using Sentinel imagery, a U-Net convolutional neural-network machine learning algorithm was employed to generate land classification and land-use categories for seasonal images.
Additionally, indices for vegetation and water were derived from the same images.
A novel data extraction method was employed to ensure remo... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
A predictive model of mosquito abundance by these micro-environmental factors was developed using a suite of 5 different machine learning statistical techniques.
Random Forest achieved the highest overall accuracy of 95% and was used to derive a predicated mosquito risk map for the entire county.
This study demonstra... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
We believe integration of this technology into routine vector control activities will work to reduce the burden of mosquito-borne disease and limit insecticide residence through targeted abatement.1316GROUND MOSQUITO ADULTICIDE FIELD TRIAL OKLAHOMACaio Martinelle B. Franca1, Brianna Temby1, Tre Williams2, Mandy Dixon3,... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Assessing the level of insecticide resistance in a given mosquito population is essential for the efficient management and control of mosquito vectors.
Three populations were analyzed in this pioneer study in Oklahoma: an adult Mixed wild population from the city of Bethany, and two laboratory-reared larvae population... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Our approach consisted of a ground adulticide field trial in a field station arranged in a 3x3 grid.
The adulticides used were the organophosphate Fyfanon EW and pyrethroid PermaSease 4-4.
Cages were collected 20-30 minutes following spray off and mortality readings were taken in the following 12hr and 24hr.
Conside... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Survivors were stored at -80 ºC and enzyme activity assays of P450 cytochrome, α- and β-carboxylesterases, and glutathione S-transferases astmh.org 416were determined using a laboratory susceptible strain as control.
Mortality data was not adjusted using Abbott’s formula because control mortality exceeded 20%. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
For all populations in response to Fyfanon EW, the highest 24hr mortality was achieved at 100 ft whereby the Culex population had the highest overall mortality in response to Fyfanon EW, ranging from 77.78%-84.13%.
The 24hr mortality at all distances for all populations in response to PermaSease 4-4 was high (88.36-10... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Minute recovery was observed between the 12 and 24 hr mortality readings, suggesting that kdr is not an abundant insecticide resistance mechanism in these populations.1317IMPROVED GLUE FOR TRAPPING BLACK FLIESMichael Banfield1, Monsuru Adeleke2, David W. Oguttu3, Ruth Dixon4, Louise Hamill4, Thomas Unnasch51BanfieldBio... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Preliminary studies in both Uganda and Nigeria revealed that a BanfieldBio Inc. (BBI) glue with an ultraviolet (UV) component performed equal to, or better, than the TAD (formerly Tanglefoot) formulations being used.
Our team then compared the efficacy of six different glue types on EWTs in two separate locations per ... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
We tested two glues from Trecé Adhesives Division (TAD) (All-weather and Hand Applied) and four from BBI (BioGlue and Autocidal Gravid Ovitrap (AGO)).
Both BBI formulas were tested with and without a UV pigment component.
Using a Latin square design, glues were rotated between six separate sites per location each day... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
In Uganda, the TAD All Weather formulation outperformed the other glues for both sites.
The BioGlue (UV) was the next best in the proportion of flies caught.
Among the BBI formulations, the glues with a UV component outperformed their counterparts.
In Nigeria, both TAD formulations and the UV-infused glues were deem... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
In Uganda, the stability results were similar, but the UV-infused glues required more recoats than the TAD formulations.
Based on the preliminary study, it was hypothesized that the UV-infused glues would outperform their counterparts and the TAD formulations, but, while the former was found to be true, the latter was... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Based on both the stability of the glue and the proportion of flies caught compared to the other glue formulations, the TAD All Weather formulation appears to have the highest efficacy for catching black flies.
This study demonstrated that infusing a glue with UV can increase the efficacy of the glue, but other compon... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Using the TAD All Weather formulation provided a demonstrated increase in EWT efficacy over the previous adhesives used.1318INSECTICIDE RESISTANCE OF AEDES ALBOPICTUS INVADED MOZAMBIQUEKawane Uruma1, Kyoko Futami1, Hitoshi Kawada1, Nelson Cuamba2, Noboru Minakawa11Nagasaki University, Nagasaki, Japan, 2National Malaria... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Although Ae.
albopictus has already established in West Africa, it had not established in the continental part of East Africa until its establishment was confirmed in southern Mozambique by 2019.
A recent population genetic study suggests that the Mozambique population originated in Southeast Asia.
Because Asian pop... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
In the analyses, we used Ae.
albopictus samples collected at Maputo, the capital city of Mozambique in 2019.
The genetic analysis found that some individuals had F1534C knockdown resistance (kdr) mutation.
The WHO Tube Assay showed that the F10 generation with F1534C mutation are resistant to 0.25% permethrin, the o... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
In the bioassay with 0.25% permethrin, the F1534C homozygous individuals had a higher survival rate (50%, n = 4) after 24 hours than the wild-type F1534 homozygous individuals (19%, n = 22).
However, the number of homozygous individuals with the mutation was small, and nearly 20% of wild type individuals survived. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
We are conducting additional experiments with a larger sample size, and testing whether the population has another resistant mechanism such as metabolic resistance.1319CHARACTERIZING PYRETHROID RESISTANCE IN FIELD-COLLECTED ANOPHELES GAMBIAE S.S AND AN. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
ARABIENSIS FROM 11 DISTRICTS IN UGANDAHenry D. Mawejje1, David D. Weetman2, Adrienne Epstein2, Amy D. Lynd2, Moses R. Kamya1, Jimmy Opigo3, Catherine Maiteki-Sebuguzi3, Jo Lines4, Grant Dorsey5, Martin J. Donnelly2, Sarah G. Staedke41Infectious Diseases Research Collaboration, Kampala, Uganda, 2Liverpool School of Trop... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Here, An.
gambiae s.s and An.
arabiensis were collected from 11 districts stratified by IRS status namely; No IRS, IRS stopped (2009-2014, then 2017) and IRS active (2014-2019).
In An.
gambiae, mosquitoes were genotyped for resistance mutations: Vgsc-1014S, Vgsc-1014F, Cyp6aa1, Cyp6p4, ZZB-TE, Cyp4j5, and Coeae1d.
... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
gambiae s.s. and 60% (1648) An.
arabiensis.
Mortality to permethrin was significantly higher in An.
arabiensis compared to An.
gambiae in No IRS (67.7% vs 16.1%, OR 0.10, 95% CI:0.05-0.19, p<0.0001) and IRS stopped (63.6% vs 11.3%, OR 0.20, 95% CI: 0.10-0.38, p<0.0001).
Higher mortality to deltamethrin compared to... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
arabiensis.
In An.
gambiae, PBO showed partial restoration of susceptibility to permethrin (16.1% vs 54.5%, OR 6.80, 95% CI: 3.08-15.05, p<0.0001) and deltamethrin (24.6% vs 55.6%, OR 3.83, 95% CI: 2.01-7.31, p<0.0001) with near restoration of susceptibility in An.
arabiensis.
The Vgsc-1014S mutation approached fix... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
An.
arabiensis were predominantly (~97%) wild type-Vgsc-1014L.
Notably, Vgsc-1014S/F resistant alleles were associated with An.
gambiae survival to deltamethrin (OR 3.44, 95% CI: 1.02-11.57, p=0.046.
The Cyp4j5 mutation and the triple mutation (Cyp6aa1, Cyp6p4 and ZZB-TE) were associated with survival to Deltamethr... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
In this study, molecular markers showed consistency with association to pyrethroid resistance advocating for the need to have resistance marker screening in entomological surveillance programs.astmh.org 1320ABSENCE OF INSECTICIDE SELECTION IN A PYRETHROID RESISTANT AEDES AEGYPTI STRAIN: RESPONSE IN SUSCEPTIBILITY, CROS... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
This resulted in selection of resistance mechanisms that either reduced pyrethroid binding to their target site, the voltage-gated sodium channel (VGSC) or increased their metabolism by detoxification enzymes.
Since few insecticide classes are available for public health, management of resistance has been proposed as ... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Management of resistance relies in the assumption that negative fitness is associated with resistance and when insecticides are no longer in use, susceptibility is restored.
To test this hypothesis in the laboratory, we characterized the resistance profile of a pyrethroid resistant Ae.
aegypti strain from Mexico (VF1... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
In parallel, we maintained a permethrin resistant strain by artificial selection (VPERM).
By the 27th generation, VSUS resistance ratio (RR) to permethrin declined from 55-fold to 2.4-fold and deltamethrin declined from 73 to 1.24-fold.
Malathion RR was maintained at 2-fold.
Chlorpyrifos RR declined from 6.8 to 0.85... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
By comparing the exomes of the 27th generation of VSUS and VPERM, we identified the serine/arginine protein, ovochymase-2|polyserase-2 and transmembrane serine protease 9 with high association values in chromosome 1.
Insecticide detoxification genes included the glutathione S-transferase theta-1 and cytochrome CYP12F8... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
In chromosome 3, the mitochondrial genome maintenance exonuclease, angio-associated migratory cell protein, VGSC, CYP6AK1 and carboxy/choline esterase CCEunk1O differed significantly among strains.
Further research will elucidate the functional role of these genes in conferring permethrin resistance.417that the mosqui... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
While the other resistant field colony presented a lower susceptibility to infection and a higher expression of immune markers.
Our results indicated that these insecticide resistance mechanisms could potentially affect susceptibility to VDEN-2 infection.
In addition to highlighting the importance of understanding th... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
aegypti to evaluate the possible consequences in the transmission of disease-causing pathogens in mosquitoes that survive the control methods commonly used in public health.1322A SHUT-DOWN IN EXPRESSION OF A MALE-SPECIFIC AMINOPEPTIDASE IMPEDES MALE REPRODUCTIVE SUCCESS OF THE MOSQUITO CULEX PIPIENSTatyana Martynova, C... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
However, little is known about the molecular functions of male accessory glands in this species.
After using RNA-seq and qRT-PCR analysis to identify sex-specific genes during pupal and adult stages of the mosquito Culex pipiens, we then examined male accessory gland tissues of adult male mosquitoes using RNA-seq and ... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
RNAi knockdown of the male-specific gene aminopeptidase (CPIJ003539) was then performed to show the functional role in the male accessory glands in Cx.
pipiens.
Observations showed clear differences in knockdown tissue under light microscopy.
In-cage fertility assays were also performed to assess fertility and fecun... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The percentage of sperm in the spermatheca was recorded after dissection to assess significant differences in insemination rate between knockdown versus wild type Cx. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
pipiens males.13231321DIAPAUSE-SPECIFIC GENE EXPRESSION IN THE FAT BODY OF THE MOSQUITO CULEX PIPIENSVECTOR COMPETENCE AND IMMUNE RESPONSE IN PYRETHROID-RESISTANT AEDES AEGYPTI FOR DENV-2 INFECTIONXueyan Wei, Cheolho SimBaylor University, Waco, TX, United StatesPatricia Penilla1, Luis A. Caraveo-Centeno1, Humberto Lanz... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Depending on their resistance levels and different mechanisms it is expected to affect the interaction between mosquitoes and the pathogens they transmit.
In this study we investigated whether resistance to pyrethorids in Ae.
aegypti has an effect on its vector competence and immune response to DENV-2. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
High levels of esterases and MFO enzymes, as well as kdr mutations (Ile 1016 and Cys 1534) were present in two field mosquito populations from Tapachula, Mexico, compared to the New Orleans susceptible strain.
The field mosquitoes and the laboratory colony were infected with DENV-2.
Viral load detection was performed... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The statistical analysis indicated Northern house mosquito Culex pipiens is a major disease vector for West Nile virus that enter mosquito diapause during winter to survive inimical conditions by inducing a series of unique diapause phenotypes such as stress tolerance, lipid accumulation, and extended longevity.
Forkh... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Since the fat body plays a crucial role in the storage and release of nutrients, synthesis of hemolymph proteins, and cross-talk with the endocrine system, the fat body-specific genes play a key role in the diapause program in this species.
Although gene expression profiles have been studied in diapausing and non-diap... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
pipiens.
Here, we use RNA sequencing to contrast gene expression profiles between non-diapause and diapause to identify transcriptional changes specific to the fat body of Cx.
pipiens.
Functional annotation of upregulated genes in diapausing mosquito fat body identified genes related to carbohydrate metabolism, plec... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Quantitative real-time PCR validation of candidate genes from astmh.org 418RNA-seq analysis is used to verify the upregulation of these genes in the diapausing condition.
Our findings provide promising insights into the discovery of potential regulators during diapause in Cx. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
pipiens.1324RAPID EVOLUTION OF TRANSCRIPTION REGULATION BETWEEN ANOPHELES GAMBIAE COMPLEX MEMBER SPECIES IMPACTS HYBRID STERILITY AND INTROGRESSIONKevin C. Deitz1, Michel A. Slotman21American Museum of Natural History, New York, NY, United States, 2Avans University, Breda, NetherlandsA major goal in evolutionary biolog... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
While variation in complex traits has been mapped to quantitative trait loci, individual genes, amino acid substitutions, or cis-regulatory variants, our understanding of how most complex traits are regulated by interacting gene networks remains incomplete.
Our ability to understand epistatic interactions between loci... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Gene expression differences between malaria vectors impact their behaviors, physiologies, and capacity to transmit malaria to humans.
Cis-regulatory elements (CREs) and trans-acting factors (TAFs) interact to modulate the expression of their target genes.
As species diverge, selection acts to fix genetic variation in... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
In this study we compare allele-specific expression of Anopheles gambiae species complex F1 hybrids to pure species and quantify gene expression divergence in cis- and trans-.
We integrate these results into a QTL analysis of hybrid female sterility between two An. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
gambiae complex member species and highlight putative sterility loci which serve as barriers to introgression between these important malaria vectors.1325ASSESSING THE ROLE OF FAMILY-LEVEL VARIATION AND HEAT SHOCK GENE EXPRESSION IN THE THERMAL STRESS RESPONSE OF THE MOSQUITO AEDES AEGYPTIFhallon Ware-Gilmore1, Mario N... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The mosquito Aedes aegypti is the primary vector of many human disease-causing viruses worldwide, and its geographic range is expanding, in part, due to changing climate.
The capacity for this species to respond adaptively to change will be a key component determining its success in warming environments.
It is not cl... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Nor are the physiological response underpinning thermal tolerance well understood.
To cope with thermal stress, in the short term, cells induce a plastic response referred to as the Heat Shock Response (HSR).
Heat Shock Proteins characterize part of the HSR, and the contribution of these thermotolerance genes to the ... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Using a high throughput physiological ‘knockdown’ assay, we investigated mosquito thermal tolerance in the framework of a family-based breeding design, with the goal of characterizing the role of genetic variation for the trait.
While families representing the extreme ends of the distribution differed in their knockdo... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
We then explored whether siblings in the families representing the extremes in performance for knockdown time differed in their heat shock response, as assayed via the expression of multiple Heat Shock Protein genes (HSP’s 26, 83, 70).
We discovered that after exposure to heat stress, families with higher thermal tole... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
This pattern suggests that other mechanisms of heat tolerance may be more important than HSP’s, and that increased expression may be a sign of poor adaptation to heat stress. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Dissecting the genetic mechanisms that underpin thermal tolerance is key to assessing the future impact that climate change will have on mosquito-borne disease transmission.1326RNA-SEQ-POP: EXPLOITING THE SEQUENCE IN RNA-SEQ - A SNAKEMAKE WORKFLOW REVEALS PATTERNS OF INSECTICIDE RESISTANCE IN ANOPHELES GAMBIAESanjay Cu... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Here, we present a scalable snakemake workflow, RNA-Seq-Pop, which comprehensively analyses RNA-Sequencing data in a reproducible manner, performing quality control and differential expression analyses, as well as calling genomic variants and generating a range of useful statistics.
We demonstrate the utility of the w... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
We provide additional modules specifically of interest for An.
gambiae s.l.The workflow reports allele frequencies of variants of interest, detecting a dramatic shift in frequency of VGSC-995S (26→100%) and Gste2-119V (11→53%) during insecticide selections.
RNA-Seq-Pop summarises genetic diversity, capturing effects ... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
RNA-Seq-Pop can estimate recent ancestry within the gambiae complex; we show that the reference An.
gambiae Kisumu strain, contains a large degree of An.
coluzzii ancestry on the autosomes.
The workflow can also determine the karyotype of common chromosomal inversions - we show that the 2La chromosomal inversion ros... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
RNA-Seq-Pop is designed for ease-of-use, and does not require programming skills to configure and run.
We hope that the workflow will be a useful tool to facilitate reproducible, comprehensive transcriptomic studies both in An.
gambiae, and across other taxa.1327YEAST INTERFERING RNA LARVICIDES FACILITATE SCALED REAR... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
MOSQUITOESTeresia Njoroge, Keshava Mysore, Molly Duman ScheelIndiana University School of Medicine, South Bend, IN, United StatesSeveral emerging population-based mosquito control technologies, such as the sterile insect technique (SIT) and incompatible insect technique (IIT), will benefit from the establishment of eff... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Small interfering RNA- (siRNA-) based screens conducted in Aedes aegypti larvae revealed multiple genes that are required for survival of female larvae or the development of female-specific traits.
Several of the siRNAs identified significantly increased male:female sex ratios by causing female larval death, while oth... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
A number of the genes identified in the A. aegypti screen are well conserved in multiple species of Diptera, including vector mosquitoes.
Silencing orthologs of these genes in other mosquitoes, including Aedes albopictus, Anopheles gambiae, Culex pipiens, and Culex quinquefasciatus, resulted in significantly increased... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Larval consumption of Saccharomyces cerevisiae (yeast) strains engineered to express interfering RNA corresponding to these genes results in increased 5 male: 1 female ratios in surviving astmh.org adults.
Incorporation of the yeast larvicides into mass rearing protocols for culturing each species of mosquito is facil... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Initial efforts to scale yeast fermentation and drying have been successful and are being further expanded to facilitate the global distribution of this technology to support mosquito control programs worldwide. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
These efforts, the putative functions of the genes identified in these screens, and the implications of these findings for the study of mosquito sex chromosome evolution will be discussed.1328A ZONE OF INHIBITION ASSAY TO SCREEN FOR HUMORAL ANTIMICROBIAL ACTIVITY IN ANOPHELES GAMBIAE HEMOLYMPHBianca Morejon, Kristin Mi... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The transcription of innate immunity genes encoding for AMPs is highly dependent on several signaling cascade pathways, such as the Toll pathway.
In mosquitoes, AMPs hold special interest as their upregulation limits the growth of variety of mosquito-borne pathogens, including malaria parasites.
However, the regulati... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Here, we establish a zone of inhibition assay (ZOI) coupled with RNAi to identify the contribution of AMPs and components of the Toll pathway to humoral antimicrobial activity of Anopheles gambiae hemolymph.
With this assay, we show that Micrococcus luteus challenge induces antimicrobial activity in the adult female m... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Moreover, by silencing CACTUS, REL1 and MyD88 we demonstrate that CACT kd induces antimicrobial activity in the mosquito hemolymph, whereas the antimicrobial activity in REL1 kd and MyD88 kd is reduced after challenge, formally establishing a role of MyD88 upstream of CACT in antimicrobial immunity in An.
gambiae.
In... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Together, our results establish ZOI assays as a scalable medium throughput assay to identify components of the humoral immune system and their regulation in this important mosquito vector species.1329IDENTIFICATION OF CYCLE TARGETS THAT CONTRIBUTE DIVERSE FEATURES OF CIRCADIAN RHYTHMS IN THE MOSQUITO CULEX PIPIENSPrabi... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
These rhythmic patterns are believed to be controlled by the functional endogenous light-entrainable circadian clock comprising positive and negative regulators that works in a transcription-translation feedback loop.
Moreover, these mosquitoes undergo seasonal diapause in response to the short photoperiod of late sum... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
However, the exact genetic and cellular mechanism behind the clock gene-mediated activity pattern and developmental switching to diapause still need to be completely unraveled.
To determine the possible linkage between clock genes and downstream processes, we used ChIP-sequencing to identify the direct targets of one ... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The nearest genes corresponding to the peaks within the 1Kb upstream of the transcription start site in promoter with the predicted binding site, E box element (CACGTG), were extracted for each binding site, resulting in a dataset comprising the target genes possibly regulated by CYC.
The top five hundred candidates w... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Genes related to different processes, including insect hormone synthesis, and feeding behavior regulation, were selected and tested.
ChIP-quantitative PCR and quantitative real-time PCR validated ten candidate genes related to the behaviors mentioned above. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
These genes showed a significantly high expression in dawn compared to dusk in accordance with the expression pattern of cyc mRNA level and are thus strong candidates for mediating the circadian rhythmicity.1330A NEW DATABASE OF STAKEHOLDER AND PUBLIC ENGAGEMENT EFFORTS FOR BIO-CONTROL TECHNOLOGIES FOR CONTROLLING VECT... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The effective and sufficient engagement is especially important for genetic bio-control technologies that involve mass releases of modified organisms, where potential risks and benefits are likely to spread past local circumstances and initial jurisdictions.
While there are many examples of efforts to engage stakehold... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
This makes it difficult to learn from past efforts when assessing needs and opportunities in new contexts.
We compiled documented examples of stakeholder, community and public engagement efforts for bio-control technologies for the control of vector borne diseases and developed a database to catalogue this information... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The database will allow interested parties such as technology developers, vector control and public health specialists, regulatory and government officials, and funders to learn from past engagement efforts and identify opportunities for future work in different contexts.1331EXPERTS INSIGHTS ON THE ROLE OF STAKEHOLDER ... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
However, concerns and uncertainty surrounding these technologies have led to increasing calls to ensure sufficient and effective community and stakeholder engagement.
This study aimed to explore insights and recommendations from key stakeholders on how community and stakeholder engagement (CSE) could be fed into decis... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Discussions were centered around how CSE can be used to build partnerships and facilitate knowledge-sharing in different astmh.org 420contexts where these technologies will likely be deployed.
Participants framed the role of CSE in informing decisions in different ways.
Gene drive developers based their insights on h... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
On the other hand, vector control specialists focused their discussions on how the technologies could fit within current vector control contexts and complement ongoing efforts.
Differently, policy contributors focused on how to build a sustainable process to ensure local ownership and sustainability of the technologie... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The findings point to the need for effective strategies for coordinating these priorities in order to develop gene drive technologies that are not only effective, but also meet the community needs and integrate with the ongoing local vector control efforts.1332AEDES AEGYPTI PHOSPHOLIPASE-C MUTANT LACKS LIGHT-EVOKED BEH... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The Aedes aegypti β-class phospholipase C (PLCβ) is the ortholog of Drosophila NORPA PLCβ essential for light detection in both larval and adult photoreceptors.
To study visual contributions to Aedes behaviors, we created CRISPR-CAS9 induced mutations within the Aedes aegypti PLCβ gene.
A homozygous viable Aedes muta... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The predicted 124 kD protein PLCβ was readily detected in protein blots of wild type head extracts but not norpACAT head extracts.
In histological analysis of retinal tissue, PLCβ protein was detected in the rhabdomeres of wild type photoreceptor cells, the expected location, but not anywhere in the retina of norpACAT... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
This response was diminished, being notably slow to respond and slow to recover from a light stimulus relative to the wild type control.
To determine if the norpACAT mutant is deficient in visual responses, we developed assays to detect larval light-triggered startle responses, larval light-avoidance behavior, and adu... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
The norpACAT mutant shows normal circadian activity and locomotion, demonstrating that the absence of immediate light-triggered responses is not due to general lethargy. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Our results demonstrate that the norpACAT mutant is defective in acquisition of visual information and therefore provides a genetic model capable of discerning the role of vision in complex mosquito behaviors.1333GENETIC DIVERSITY AND POPULATION STRUCTURE OF ANOPHELES FUNESTUS BASED ON MITOCHONDRIAL DNA MARKERS (MTDNA-... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Adult mosquitoes were collected using mechanical aspirators in four sites (Bungoma, Port Victoria, Kombewa and Migori) across four counties in western Kenya.
All samples were morphologically identified as An.
funestus s.l.
The COI and COII genes were amplified, sequenced and analyzed to identify sibling species and ... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Hundred and Sixty (160) An.
funestus s l specimens (40 from each site) were used for species identification, genetic structure and gene flow.
The COI gene showed that An.
funestus constitutes 96% of all the specimens identified.
Other members of the An. | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
Other members of the An.
funestus complex identified includes Anopheles funestus-like Anopheles longipalpis, Anopheles parensis and Anopheles vaneedeni.Both genes exhibit high haplotype diversity (COI, Hd=0.99; COII, Hd=0.98) but low nucleotide diversity (COI, Π=0.03; COII, Π=0.02). | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
A minimal level of genetic differentiation was observed between Port Victoria and Bungoma (FST = 0.01512, P= 0.00000), Port Victoria and Kombewa (FST = 0.03135, P=0.00000), Migori and Kombewa (FST = 0.03568, P=0.000), Migori and Bungoma (FST = 0.01621, P=0.01802) but no genetic differentiation between Port Victoria and... | https://docs-lawep.s3.us-east-2.amazonaws.com/1710413448560.pdf | https://www.astmh.org/getmedia/65cc0d8d-1208-4d9a-9f77-734d40de4c02/ASTMH-2022-Annual-Meeting-Abstract-Book.pdf | Ukraine |
End of preview. Expand in Data Studio
README.md exists but content is empty.
- Downloads last month
- 2