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Please summerize the given abstract to a title
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Bridging Disciplines: Applications of Forensic Science and Industrial Hemp
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Forensic laboratories are required to have analytical tools to confidently differentiate illegal substances such as marijuana from legal products (i.e., industrial hemp). The Achilles heel of industrial hemp is its association with marijuana. Industrial hemp from the Cannabis sativa L. plant is reported to be one of the strongest natural multipurpose fibers on earth. The Cannabis plant is a vigorous annual crop broadly separated into two classes: industrial hemp and marijuana. Up until the eighteenth century, hemp was one of the major fibers in the United States. The decline of its cultivation and applications is largely due to burgeoning manufacture of synthetic fibers. Traditional composite materials such as concrete, fiberglass insulation, and lumber are environmentally unfavorable. Industrial hemp exhibits environmental sustainability, low maintenance, and high local and national economic impacts. The 2018 Farm Bill made way for the legalization of hemp by categorizing it as an ordinary agricultural commodity. Unlike marijuana, hemp contains less than 0.3% of the cannabinoid, Δ(9)-tetrahydrocannabinol, the psychoactive compound which gives users psychotropic effects and confers illegality in some locations. On the other hand, industrial hemp contains cannabidiol found in the resinous flower of Cannabis and is purported to have multiple advantageous uses. There is a paucity of investigations of the identity, microbial diversity, and biochemical characterizations of industrial hemp. This review provides background on important topics regarding hemp and the quantification of total tetrahydrocannabinol in hemp products. It will also serve as an overview of emergent microbiological studies regarding hemp inflorescences. Further, we examine challenges in using forensic analytical methodologies tasked to distinguish legal fiber-type material from illegal drug-types.
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Characterizing heat mitigation strategies utilized by beef processors in the United States
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During lairage at slaughter plants, cattle can be exposed to extreme heat conditions from pen densities and holding pen microclimates. While research outlining heat mitigation strategies used in other sectors of the beef supply chain is available, there is no published data on the use of heat mitigation strategies at slaughter plants. The objective of this study was to characterize short-term heat mitigation strategies used by commercial beef slaughter plants in the United States. Twenty-one beef slaughter plants, representing an estimated 60% of beef slaughter in the United States, were included in the study. All plants indicated use of at least one heat mitigation strategy, and five of them used more than one type. Sprinklers/misters were the most commonly used heat mitigation type (n = 17, 81%), and fans were the least common type (n = 4, 19%). Shade usage was present in several plants (n = 7, 33%), ranging from barn style roofs to shade cloths. Respondents indicated that they believed heat mitigation strategies provide benefits both to cattle well-being and meat quality outcomes. Future research should focus on the effectiveness of these techniques in improving animal well-being and quality outcomes in the slaughter plant environment and protocols for optimum implementation.
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Evaluation of Different Interstimulus Rinse Protocols on Smoke Attribute Perception in Wildfire-Affected Wines.
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Wildfires produce smoke that can carry organic compounds to a vineyard, which are then absorbed by the grape berry and result in wines with elevated levels of smoke-related phenols. These phenols have been found to have a large impact on the flavor of wines, being the cause of a smokey flavor with a lasting ashy aftertaste. When evaluating the sensory profile of these wines, there is an observed problem due to the lasting nature of these undesirable attributes and potential flavor carryover between samples. Through the use of standard and temporal attribute check-all-that-apply, this research desires to better understand the impact of smoke on the sensorial profiles of wines with various levels of smoke phenols (high, moderate, and low). Additionally, through the employment of different interstimulus protocols, the effectiveness of rinses on diminishing the smoke flavor in wines and optimal time separation were investigated. It was determined that a 1 g/L pectin rinse in between samples with a 120 s separation is optimal to ensure the removal of smoke attribute perception. This work also indicated the need to look deeper at the effects of the in-mouth hydrolysis of glyconjugate phenols that impact overall smoke flavor.
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Evaluating the contribution of synthetic fungicides to cereal plant health and CO2 reduction targets against the backdrop of the increasingly complex regulatory environment in Europe
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The global COVID pandemic has impacted the world in ways and at a scale that few could have predicted, with many industries severely disrupted. Despite this, crops were sown and harvested, food was produced and agriculture continued to function, albeit it with many logistical challenges. Plant health lies at the heart of preventing crop losses through a combination of varietal resistance and agronomic practices. In the case of foliar plant diseases in wheat, varietal resistance plays a key role, but the use of synthetic fungicides is essential to minimize crop losses. European arable production faces a dilemma: how to contribute and maintain global food supplies but at the same time decrease emissions of greenhouse gases (GHGs), reduce inputs potentially harmful to biodiversity, society and the environment while ensuring no more land is brought into production. Throughout history, major disruptions in society have led to big steps in agricultural innovation. Presently, the major disruptive forces in Europe are not just a result of the COVID pandemic but the increasingly urgent need to address climate change. Within the European Green Deal, the Farm‐to‐Fork strategy is in place to help achieve climate neutrality by 2050, aiming for a reduction of GHG emissions of 55% by 2030. To achieve this, there will need to be a major adjustment to how food is produced, a realignment in plant health strategies and accelerated innovation across the agricultural sector. This paper aims to evaluate how synthetic fungicides presently contribute to plant health (mainly wheat) and food production as well as the management of GHG emissions. In addition, it explores the future challenges and prospects for their positive contribution in achieving global food security alongside emerging innovative technologies. [ABSTRACT FROM AUTHOR] Copyright of Plant Pathology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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Lessons and surprises from an inter- island re-introduction of the critically endangered raso lark alauda razae of cape verde
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Confined to a single island where its small population fluctuates in response to rainfall, the Raso lark is likely to remain Critically Endangered unless a second population becomes established This paper reports translocations of larks, 37 in 2018 and 33 in 2019, to the nearby island of Santa Luzia where the species existed until about 500 years ago Both islands lie within the Reserva Natural Integral de Santa Luzia While the hard release protocol proceeded without mishap, problems with radio-tagging the released birds were encountered However early indications are encouraging;the released birds have bred, and their annual survival is not substantially worse than that of larks on the source island, Raso Unexpectedly, the study documented several natural, unassisted movements of larks from Raso to Santa Luzia (and, less unexpectedly, of translocated birds returning to Raso) The ultimate outcome of the project remains uncertain since eradication of Santa Luzia’s introduced cats, possible predators of the larks, was interrupted when the island was evacuated because of the Covid-19 pandemic © Paul Donald
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Long-Term Monitoring of Amphibian Populations of a National Park in Northern Spain Reveals Negative Persisting Effects of Ranavirus, but Not Batrachochytrium dendrobatidis
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Amphibians are the most highly threatened vertebrates, and emerging pathogens are a serious threat to their conservation. Amphibian chytrid fungi and the viruses of the Ranavirus genus are causing disease outbreaks worldwide, including in protected areas such as National Parks. However, we lack information about their effect over amphibian populations in the long-term, and sometimes these mortality episodes are considered as transient events without serious consequences over longer time-spans. Here, we relate the occurrence of both pathogens with the population trends of 24 amphibian populations at 15 sites across a national Park in northern Spain over a 14-year period. Just one out 24 populations presents a positive population trend being free of both pathogens, while seven populations exposed to one or two pathogens experienced strong declines during the study period. The rest of the study populations (16) remain stable, and these tend to be of species that are not susceptible to the pathogen present or are free of pathogens. Our study is consistent with infectious diseases playing an important role in dictating amphibian population trends and emphasizes the need to adopt measures to control these pathogens in nature. We highlight that sites housing species carrying Ranavirus seems to have experienced more severe population-level effects compared to those with the amphibian chytrid fungus, and that ranaviruses could be just as, or more important, other more high-profile amphibian emerging pathogens.
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Ranking the risk of animal-to-human spillover for newly discovered viruses
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The death toll and economic loss resulting from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic are stark reminders that we are vulnerable to zoonotic viral threats. Strategies are needed to identify and characterize animal viruses that pose the greatest risk of spillover and spread in humans and inform public health interventions. Using expert opinion and scientific evidence, we identified host, viral, and environmental risk factors contributing to zoonotic virus spillover and spread in humans. We then developed a risk ranking framework and interactive web tool, SpillOver, that estimates a risk score for wildlife-origin viruses, creating a comparative risk assessment of viruses with uncharacterized zoonotic spillover potential alongside those already known to be zoonotic. Using data from testing 509,721 samples from 74,635 animals as part of a virus discovery project and public records of virus detections around the world, we ranked the spillover potential of 887 wildlife viruses. Validating the risk assessment, the top 12 were known zoonotic viruses, including SARS-CoV-2. Several newly detected wildlife viruses ranked higher than known zoonotic viruses. Using a scientifically informed process, we capitalized on the recent wealth of virus discovery data to systematically identify and prioritize targets for investigation. The publicly accessible SpillOver platform can be used by policy makers and health scientists to inform research and public health interventions for prevention and rapid control of disease outbreaks. SpillOver is a living, interactive database that can be refined over time to continue to improve the quality and public availability of information on viral threats to human health.
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Please summerize the given abstract to a title
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232,708
Bird-window collisions: Mitigation efficacy and risk factors across two years
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BACKGROUND: Research on bird-window collision mitigation is needed to prevent up to a billion bird fatalities yearly in the U.S. At the University of Utah campus (Salt Lake City, Utah, USA), past research documented collisions, especially for Cedar Waxwings (Bombycilla cedrorum) drawn to fruiting ornamental pears in winter. Mirrored windows, which have a metallic coating that turns window exteriors into mirrors, had frequent collisions, which were mitigated when Feather Friendly®bird deterrent markers were applied. Bird-friendly windows–ORNILUX®ultraviolet (UV) and fritted windows–also reduced collisions when data were collected across fall and winter. Extending this prior research, we evaluated additional mitigation and tested the replicability of effects for pear trees, mirrored windows, and bird-friendly windows across two years. METHODS: Using published data from eight buildings monitored for collisions in year 1 (Fall and Winter, 2019–2020), we added another year of monitoring, Fall and Winter, 2020–2021. Between years, Feather Friendly®mitigation markers were added to collision-prone areas of two buildings, including both mirrored and transparent windows. RESULTS: The two buildings that received new Feather Friendly®mitigation had significantly fewer collisions post-mitigation. Control areas also had nonsignificant decline in collisions. The interaction of area (mitigation vs. control) by time (year 1 vs. 2) was significant, based on generalized estimating equations (GEE). The total yearly collisions across all eight buildings declined from 39 to 23. A second GEE analysis of all 8 buildings showed that mirrored windows, pear trees, and bird-friendly windows were each significant when analyzed separately. The best-fit model showed more collisions for mirrored windows and fewer collisions for bird-friendly windows. We found pear tree proximity to be related to more collisions in winter than fall. In addition, pear trees showed reduced collisions from year 1 to 2, consistent with new mitigation for two of three buildings near pear trees. DISCUSSION: Feather Friendly(®) markers can mitigate collisions with transparent windows, not only mirrored windows, compared to unmitigated areas over 2 years. Results also underscore the dangers of pear tree proximity and mirrored windows and the efficacy of bird-friendly windows. Thus, bird collisions can be prevented by window mitigation, permanent bird-friendly windows, and landscape designs that avoid creating ecological traps.
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232,710
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232,735
Roadmap to sustainable carbon-neutral energy and environment: can we cross the barrier of biomass productivity?
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232,736
The total number of inhabitants on the Earth is estimated to cross a record number of 9 × 10(3) million by 2050 that present a unique challenge to provide energy and clean environment to every individual. The growth in population results in a change of land use, and greenhouse gas emission due to increased industrialization and transportation. Energy consumption affects the quality of the environment by adding carbon dioxide and other pollutants to the atmosphere. This leads to oceanic acidification and other environmental fluctuations due to global climate change. Concurrently, speedy utilization of known conventional fuel reservoirs causes a challenge to a sustainable supply of energy. Therefore, an alternate energy resource is required that can maintain the sustainability of energy and environment. Among different alternatives, energy production from high carbon dioxide capturing photosynthetic aquatic microbes is an emerging technology to clean environment and produce carbon-neutral energy from their hydrocarbon-rich biomass. However, economical challenges due to low biomass production still prevent the commercialization of bioenergy. In this work, we review the impact of fossil fuels burning, which is predominantly used to fulfill global energy demand, on the quality of the environment. We also assess the status of biofuel production and utilization and discuss its potential to clean the environment. The complications associated with biofuel manufacturing using photosynthetic microorganisms are discussed and directed evolution for targeted phenotypes and targeted delivery of nutrients are proposed as potential strategies to increase the biomass production.
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Heat stress effects on bovine sperm cells: a chronological approach to early findings.
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Testicular heat stress affects sperm quality and fertility. However, the chronology of these effects is not yet fully understood. This study aimed to establish the early sequential effects of heat stress in bull sperm quality. Semen and blood samples of Nellore breed bulls were collected and distributed into control and testicular heat stress (scrotal bags/96 h) groups. Semen samples were evaluated for sperm motility, abnormalities, plasma membrane integrity, acrosomal membrane integrity, mitochondrial membrane potential, sperm lipid peroxidation, seminal plasma lipid peroxidation, and DNA fragmentation. Blood plasma was also evaluated for lipid peroxidation. An increase in sperm abnormalities was observed 7 days following heat stress. After 14 days, sperm lipid peroxidation increased and mitochondrial membrane function, sperm motility, and plasma membrane integrity decreased. Heat stress effects were still observed after 21 days following heat stress. An increase in sperm DNA fragmentation was observed as a late effect after 28 days. Thus, the initial effects of heat stress (i.e., increasing sperm abnormalities and lipid peroxidation) suggest the presence of oxidative stress in the semen that alters mitochondrial function, sperm motility, plasma membrane integrity, and belatedly, DNA fragmentation. Although sperm abnormalities persisted and increased over time, sperm lipid peroxidation, in turn, increased only until 21 days after heat stress. In this regard, these findings provide a greater understanding of the chronological effects of experimentally induced heat stress on bovine sperm, providing valuable insights about spermatogenesis during the first 28 days following heat stress.
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Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park
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SIMPLE SUMMARY: DNA molecular techniques, including multiple microsatellite analysis and genome-wide SNP-genotyping, were used to unlock and prove the poaching of wild goats (Capra caucasica) in an area of the Caucasian mountains in Russia. ABSTRACT: Poaching is one of the major types of wildlife crime in Russia. Remnants of goats (presumably the wild endemic species, the Caucasian tur) were found in an area of the Caucasian mountains. The case study involves a suspected poacher whose vehicle was found to have two duffel bags containing pieces of a carcass, which he claimed was that of a goat from his flock. The aim of the forensic genetic analysis for this case was to (i) establish individual identity and (ii) perform species identification. DNA typing based on fourteen microsatellites revealed that STR-genotypes generated from pieces of evidence found at crime scene fully matched those obtained from the evidence seized from the suspect. The results of genome-wide SNP-genotyping, using Illumina Goat SNP50 BeadChip, provided evidence that the poached animal was a wild Caucasian tur (Capra caucasica). Thus, based on comprehensive molecular genetic analysis, evidence of poaching was obtained and sent to local authorities. To our knowledge, this case study is the first to attempt to use DNA chips in wildlife forensics of ungulates.
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Mobilome-driven segregation of the resistome in biological wastewater treatment
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Biological wastewater treatment plants (BWWTP) are considered to be hotspots of evolution and subsequent spread of antimicrobial resistance (AMR). Mobile genetic elements (MGEs) promote the mobilization and dissemination of antimicrobial resistance genes (ARGs) and are thereby critical mediators of AMR within the BWWTP microbial community. At present, it is unclear whether specific AMR categories are differentially disseminated via bacteriophages (phages) or plasmids. To understand the segregation of AMR in relation to MGEs, we analyzed meta-omic (metagenomic, metatranscriptomic and metaproteomic) data systematically collected over 1.5 years from a BWWTP. Our results showed a core group of fifteen AMR categories which were found across all timepoints. Some of these AMR categories were disseminated exclusively (bacitracin) or primarily (aminoglycoside, MLS, sulfonamide) via plasmids or phages (fosfomycin and peptide), whereas others were disseminated equally by both MGEs. Subsequent expression- and protein-level analyses further demonstrated that aminoglycoside, bacitracin and sulfonamide resistance genes were expressed more by plasmids, in contrast to fosfomycin and peptide AMR expression by phages, thereby validating our genomic findings. Longitudinal assessment further underlined these findings whereby the log2-fold changes of aminoglycoside, bacitracin and sulfonamide resistance genes were increased in plasmids, while fosfomycin and peptide resistance showed similar trends in phages. In the analyzed communities, the dominant taxon Candidatus Microthrix parvicella was a major contributor to several AMR categories whereby its plasmids primarily mediated aminoglycoside resistance. Importantly, we also found AMR associated with ESKAPEE pathogens within the BWWTP, for which MGEs also contributed differentially to the dissemination of ARGs. Collectively our findings pave the way towards understanding the segmentation of AMR within MGEs, thereby shedding new light on resistome populations and their mediators, essential elements that are of immediate relevance to human health.
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Grasping at digitalisation: turning imagination into fact in the sugarcane farming community
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Nutrient runoff from catchments that drain into the Great Barrier Reef (GBR) is a significant source of stress for this World Heritage Area. An alliance of collaborative on-ground water quality monitoring (Project 25) and technologically driven digital application development (Digiscape GBR) projects were formulated to provide data that highlighted the contribution of a network of Australian sugar cane farmers, amongst other sources, to nutrient runoff. This environmental data and subsequent information were extended to the farming community through scientist-led feedback sessions and the development of specialised digital technology (1622™WQ) that help build an understanding of the nutrient movements, in this case nitrogen, such that farmers might think about and eventually act to alter their fertilizer application practices. This paper reflects on a socio-environmental sustainability challenge that emerged during this case study, by utilising the nascent concept of digi-grasping. We highlight the importance of the entire agricultural knowledge and advice network being part of an innovation journey to increase the utility of digital agricultural technologies developed to increase overall sustainability. We develop the digi-MAST analytical framework, which explores modes of being and doing in the digital world, ranging from ‘the everyday mystery of the digital world (M)’, through digital ‘awareness (A)’, digitally ‘sparked’ being/s (S), and finally the ability of individuals and/or groups to ‘transform (T)’ utilising digital technologies and human imaginations. Our digi-MAST framework allows us to compare agricultural actors, in this case, to understand present modes of digi-grasping to help determine the resources and actions likely to be required to achieve impact from the development of various forms of digital technological research outputs.
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Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils
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Nano-bioremediation application is an ecologically and environmentally friendly technique to overcome the catastrophic situation in soil because of petroleum waste contamination. We evaluated the efficiency of oil-degrading bacterial consortium and silver nanoparticles (AgNPs) with or without fertilizer to remediate soils collected from petroleum waste contaminated oil fields. Physicochemical characteristics of control soil and petroleum contaminated soils were assessed. Four oil-degrading strains, namely Bacillus pumilus (KY010576), Exiguobacteriaum aurantiacum (KY010578), Lysinibacillus fusiformis (KY010586), and Pseudomonas putida (KX580766), were selected based on their in vitrohydrocarbon-degrading efficiency. In a lab experiment, contaminated soils were treated alone and with combined amendments of the bacterial consortium, AgNPs, and fertilizers (ammonium nitrate and diammonium phosphate). We detected the degradation rate of total petroleum hydrocarbons (TPHs) of the soil samples with GC-FID at different intervals of the incubation period (0, 5, 20, 60, 240 days). The bacterial population (CFU/g) was also monitored during the entire period of incubation. The results showed that 70% more TPH was degraded with a consortium with their sole application in 20 days of incubation. There was a positive correlation between TPH degradation and the 100-fold increase in bacterial population in contaminated soils. This study revealed that bacterial consortiums alone showed the maximum increase in the degradation of TPHs at 20 days. The application of nanoparticles and fertilizer has non-significant effects on the consortium degradation potential. Moreover, fertilizer alone or in combination with AgNPs and consortium slows the rate of degradation of TPHs over a short period. Still, it subsequently accelerates the rate of degradation of TPHs, and a negligible amount remains at the end of the incubation period.
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Wheat yield potential in controlled-environment vertical farms.
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Scaling current cereal production to a growing global population will be a challenge. Wheat supplies approximately one-fifth of the calories and protein for human diets. Vertical farming is a possible promising option for increasing future wheat production. Here we show that wheat grown on a single hectare of land in a 10-layer indoor vertical facility could produce from 700 ± 40 t/ha (measured) to a maximum of 1,940 ± 230 t/ha (estimated) of grain annually under optimized temperature, intensive artificial light, high CO2 levels, and a maximum attainable harvest index. Such yields would be 220 to 600 times the current world average annual wheat yield of 3.2 t/ha. Independent of climate, season, and region, indoor wheat farming could be environmentally superior, as less land area is needed along with reuse of most water, minimal use of pesticides and herbicides, and no nutrient losses. Although it is unlikely that indoor wheat farming will be economically competitive with current market prices in the near future, it could play an essential role in hedging against future climate or other unexpected disruptions to the food system. Nevertheless, maximum production potential remains to be confirmed experimentally, and further technological innovations are needed to reduce capital and energy costs in such facilities.
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Mapping the Distributions of Mosquitoes and Mosquito-Borne Arboviruses in China
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The geographic expansion of mosquitos is associated with a rising frequency of outbreaks of mosquito-borne diseases (MBD) worldwide. We collected occurrence locations and times of mosquito species, mosquito-borne arboviruses, and MBDs in the mainland of China in 1954−2020. We mapped the spatial distributions of mosquitoes and arboviruses at the county level, and we used machine learning algorithms to assess contributions of ecoclimatic, socioenvironmental, and biological factors to the spatial distributions of 26 predominant mosquito species and two MBDs associated with high disease burden. Altogether, 339 mosquito species and 35 arboviruses were mapped at the county level. Culex tritaeniorhynchus is found to harbor the highest variety of arboviruses (19 species), followed by Anopheles sinensis (11) and Culex pipiens quinquefasciatus (9). Temperature seasonality, annual precipitation, and mammalian richness were the three most important contributors to the spatial distributions of most of the 26 predominant mosquito species. The model-predicted suitable habitats are 60–664% larger in size than what have been observed, indicating the possibility of severe under-detection. The spatial distribution of major mosquito species in China is likely to be under-estimated by current field observations. More active surveillance is needed to investigate the mosquito species in specific areas where investigation is missing but model-predicted probability is high.
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Consumption and ocurrence of antidepressants (SSRIs) in pre- and post-COVID-19 pandemic, their environmental impact and innovative removal methods: A review
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Selective serotonin reuptake inhibitors (SSRIs) are pharmaceuticals whose consumption has increased significantly. They are prescribed as first-line treatment in mental disorders such as depression, obsessive-compulsive disorder, phobias, and anxiety; also, they are indicated as adjuvants in diseases such as fibromyalgia and bulimia nervosa. In addition to being linked to the illegal market to be consumed as recreational drugs. The relevance of this review lies in the fact that worldwide consumption has increased significantly during the COVID-19 pandemic, due to the depression and anxiety that originated in the population. As a consequence of this increase in consumption, concentrations of SSRIs in the environment have increased, and these have become a relevant issue for toxicologists due to the effects that they could generate in different organisms, both aquatic and terrestrial. For this reason, the objective of this article was to do a critical evaluation of the existing data on the characteristics and physicochemical properties of SSRIs, consumption data during the COVID-19 pandemic, its occurrence in the environment and the reports of toxic effects that have been generated in different organisms; we also conclude with an updated review of different methods that have been used for their removal. With this analysis, it can be concluded that, despite SSRIs are pharmaceutical products widely studied since their launching to the market, still currently under investigation to clarify their mechanisms of action to understand the different effects on the organisms, adverse reactions, as well as possible toxicological effects on non-target organisms. On the other hand, it has been proven that although it is already possible to eliminate a significant percentage of SSRIs in the laboratory, due to their physicochemical characteristics and their behavior in complex mixtures in the environment, they have not yet been eradicated, showing a persistence in the soil, subsoil and surface waters of the entire planet that may represent a future risk.
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Natural history of social and sexual behavior in fruit flies
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The past 2 decades have seen fruit flies being widely adopted for research on social behavior and aggression. This fruitful research, however, has not been well tied to fruit flies’ natural history. To address this knowledge gap, I conducted a field study. My goal was to inform future research conducted in artificial surroundings, and to inspire new investigations that can rely more heavily on fruit flies’ actual natural behavior. My two main novel findings were first, that flies in the field showed significant sociability, as they formed social groups rather than dispersed randomly among fruits of similar quality. Second, males showed fair levels of aggression towards each other as indicated by a lunging rate of 17 per hour, and lower rates of wing threat and boxing. Courtship was the most prominent activity on fruits, with females rejecting almost all males’ advances. This resulted in an estimated mating rate of 0.6 per female per day. Flies showed a striking peak of activity early in the mornings, even at cold temperatures, followed by inactivity for much of the day and night. Flies, however, handled well high temperatures approaching 40 °C by hiding away from fruit and concentrating activity in the cooler, early mornings. My field work highlights a few promising lines of future research informed by fruit flies’ natural history. Most importantly, we do not understand the intriguing dynamics that generate significant sociability despite frequent aggressive interactions on fruits. Males’ responses to female rejection signals varied widely, perhaps because the signals differed in information content perceived by flies but not humans. Finally, flies tolerated cold early mornings perhaps owing to fitness benefits associated with increased mating and feeding opportunities at this time. Flies were adept at handling very high temperatures under the natural daily temperature fluctuations and availability of shelters, and this can inform more realistic research on the effects of global warming on animals in their natural settings.
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Feeding ecology of bonobos living in forest‐savannah mosaics: Diet seasonal variation and importance of fallback foods
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Primates along with many other animal taxa are forced to cope with large shifts in basic ecological conditions because of rapid anthropogenically induced changes of their habitats. One of the coping strategies for primates is to adjust their diet to these changes, and several studies have demonstrated the importance of fallback resources for this. Bonobos, like chimpanzees, might be particularly vulnerable to habitat fragmentation because of their high dependence on fruit availability. Little is known, however, about bonobo feeding ecology in fragmented habitats and their use of fallback resources. In this study, we investigate diet seasonal variation and the exploitation of preferred and fallback foods in a bonobo population living in forest‐savannah mosaics. Results show that bonobos have adapted to this fragmented habitat by feeding on only a few fruit species, including an important number of non‐tree species (liana, herb and savannah shrub), in comparison to populations living in dense forests. These non‐tree plants have been defined as fallback and non‐preferred foods, which are most probably consumed to maintain high frugivory. Interestingly, we identified that preferred foods are all typical of mature forests while fallback resources are mainly found in forest edges or disturbed areas. This finding indicates that bonobos prefer to use mature forests when feeding, as they do for nesting, but extend their range use to forest areas in close proximity to humans when the availability of preferred fruits is low. Finally, we show that bonobo diet relies heavily on two abundant fallback fruits: Musanga cecropioides and Marantochloa leucantha. Other studies have demonstrated that the selection of abundant fallback resources enables primates to subsist at high densities and to maintain cohesive groups, as observed at this study site. Our findings suggest that bonobos living in forest‐savannah mosaics can be considered as staple fallback food consumers. Am. J. Primatol. 77:948–962, 2015. © 2015 Wiley Periodicals, Inc.
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Variability of metabolic, protective, antioxidant, and lysosomal gene transcriptional profiles and microbiota composition of Mytilus galloprovincialis farmed in the North Adriatic Sea (Italy)
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This study evaluates the transcriptional profiles of genes related to physiological responses in digestive glands (DG) of Mytilus galloprovincialis under the influence of seasonal changes of environmental variables, gender bias, and gonadal development. Composition of the DG microbiome was also explored. Mussels were collected across 7 months encompassing 3 seasons from a farm in the Northwestern Adriatic Sea. All gene products showed complex transcriptional patterns across seasons. Salinity, surface oxygen and transparency significantly correlate with transcriptional profiles of males, whereas in females temperature and gonadal maturation mostly explained the observed transcriptional changes. Seasonal variations and gender-specific differences were observed in DG microbiome composition, with variations resembling metabolic accommodations likely facing season progression and reproductive cycle. Results provide baseline information to improve actual monitoring strategies of mussel farming conditions and forecast potential detrimental impacts of climatological/environmental changes in the study area.
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79,672
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238,606
Bovine Immunology: Implications for Dairy Cattle
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238,607
The growing world population (7.8 billion) exerts an increased pressure on the cattle industry amongst others. Intensification and expansion of milk and beef production inevitably leads to increased risk of infectious disease spread and exacerbation. This indicates that improved understanding of cattle immune function is needed to provide optimal tools to combat the existing and future pathogens and improve food security. While dairy and beef cattle production is easily the world’s most important agricultural industry, there are few current comprehensive reviews of bovine immunobiology. High-yielding dairy cattle and their calves are more vulnerable to various diseases leading to shorter life expectancy and reduced environmental fitness. In this manuscript, we seek to fill this paucity of knowledge and provide an up-to-date overview of immune function in cattle emphasizing the unresolved challenges and most urgent needs in rearing dairy calves. We will also discuss how the combination of available preventative and treatment strategies and herd management practices can maintain optimal health in dairy cows during the transition (periparturient) period and in neonatal calves.
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80,011
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239,623
Let more big fish sink: Fisheries prevent blue carbon sequestration—half in unprofitable areas
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239,624
Contrary to most terrestrial organisms, which release their carbon into the atmosphere after death, carcasses of large marine fish sink and sequester carbon in the deep ocean. Yet, fisheries have extracted a massive amount of this “blue carbon,” contributing to additional atmospheric CO(2) emissions. Here, we used historical catches and fuel consumption to show that ocean fisheries have released a minimum of 0.73 billion metric tons of CO(2) (GtCO(2)) in the atmosphere since 1950. Globally, 43.5% of the blue carbon extracted by fisheries in the high seas comes from areas that would be economically unprofitable without subsidies. Limiting blue carbon extraction by fisheries, particularly on unprofitable areas, would reduce CO(2) emissions by burning less fuel and reactivating a natural carbon pump through the rebuilding of fish stocks and the increase of carcasses deadfall.
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239,625
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80,076
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239,818
Phylogeny matters: revisiting ‘a comparison of bats and rodents as reservoirs of zoonotic viruses’
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239,819
Diseases emerging from wildlife have been the source of many major human outbreaks. Predicting key sources of these outbreaks requires an understanding of the factors that explain pathogen diversity in reservoir species. Comparative methods are powerful tools for understanding variation in pathogen diversity and rely on correcting for phylogenetic relatedness among reservoir species. We reanalysed a previously published dataset, examining the relative effects of species' traits on patterns of viral diversity in bats and rodents. We expanded on prior work by using more highly resolved phylogenies for bats and rodents and incorporating a phylogenetically controlled principal components analysis. For rodents, sympatry and torpor use were important predictors of viral richness and, as previously reported, phylogeny had minimal impact in models. For bats, in contrast to prior work, we find that phylogeny does have an effect in models. Patterns of viral diversity in bats were related to geographical distribution (i.e. latitude and range size) and life history (i.e. lifespan, body size and birthing frequency). However, the effects of these predictors were marginal relative to citation count, emphasizing that the ability to accurately assess reservoir status largely depends on sampling effort and highlighting the need for additional data in future comparative studies.
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Predicting the global mammalian viral sharing network using phylogeography
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Understanding interspecific viral transmission is key to understanding viral ecology and evolution, disease spillover into humans, and the consequences of global change. Prior studies have uncovered macroecological drivers of viral sharing, but analyses have never attempted to predict viral sharing in a pan-mammalian context. Using a conservative modelling framework, we confirm that host phylogenetic similarity and geographic range overlap are strong, nonlinear predictors of viral sharing among species across the entire mammal class. Using these traits, we predict global viral sharing patterns of 4196 mammal species and show that our simulated network successfully predicts viral sharing and reservoir host status using internal validation and an external dataset. We predict high rates of mammalian viral sharing in the tropics, particularly among rodents and bats, and within- and between-order sharing differed geographically and taxonomically. Our results emphasize the importance of ecological and phylogenetic factors in shaping mammalian viral communities, and provide a robust, general model to predict viral host range and guide pathogen surveillance and conservation efforts.
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240,643
Early Life Fecal Microbiota Transplantation in Neonatal Dairy Calves Promotes Growth Performance and Alleviates Inflammation and Oxidative Stress during Weaning
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240,644
SIMPLE SUMMARY: Neonatal dairy calves born with an immature immune system and gut microbiota are exposed to several stressors they have to overcome early in life. Therefore, we proposed the use of gut microbiota from a pre-screened healthy adult donor adapted to the local pathogen load to perform a fecal microbiota transplantation in neonatal dairy calves. Our results suggest that early life fecal microbiota transplantation in neonatal dairy calves is a relatively safe method, influencing growth and development and possibly alleviating the stress caused by weaning procedures. ABSTRACT: This study aimed to evaluate the effects of early life fecal microbiota transplantation (FMT) on the health and performance of neonatal dairy calves. The donor was selected based on health and production records and fecal material testing negative for infectious pathogens. Sixteen healthy newborn Holstein calves were randomized to either a baseline nutritional program (CON) or 1×/d inoculations with 25 g of fecal donor material (FMT) mixed in the milk replacer (n = 8/TRT) from 8 to 12 days of age. Blood and fecal samples were collected weekly, and calves were weaned at 7 weeks of age. A TRT × Week interaction was observed in haptoglobin, which was reflected in a positive quadratic effect in FMT calves but not in CON. A trend for a TRT × Week interaction was observed in the liver function biomarker paraoxonase, which resulted in greater paraoxonase in FMT calves than CON at three weeks of age. Fecal microbial community analysis revealed a significant increase in the alpha-diversity between week 1 and week 5 for the FMT calves. These results suggest that early life FMT in neonatal calves has positive effects in mediating the inflammatory response and gut microbial maturation.
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240,645
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240,676
Aluminum bioconcentration in female Nile tilapia Oreochromis niloticus (Perciformes: Cichlidae) and the effects on pituitary gonadotropins.
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240,677
In this study, we measured aluminum (Al) bioconcentration in the brain, ovaries, and liver of Oreochromis niloticus females, and analyzed the effects of exposure to Al and acidic pH on the gene expression of follicle-stimulating hormone (βfsh) and luteinizing hormone (βlh) in these animals. Mature females were divided into 4 groups, thus being maintained for 96 h in one of the following conditions: control at neutral pH (Ctr); Al at neutral pH (Al); acidic pH (Ac), and Al at acidic pH (Al-Ac). pH alone did not influence Al bioconcentration in the brain. The animals from the Al-Ac group bioconcentrated more Al in the ovaries than those from the Al group, while no differences were observed in the liver. Aluminum bioconcentration was higher in the brain than in the liver and ovaries in Al-exposed animals (Al and Al-Ac), and higher in the brain than in the ovaries in the Ctr and Ac groups. The liver bioconcentrates more Al than the ovaries in the females from the Ctr and Ac groups. Aluminum and/or acidic pH did not alter βfsh gene expression, while βlh gene expression decreased in females from the Al group. Aluminum acted as an endocrine disruptor, suggesting deleterious effects in reproduction that could result in ovulation failure. Aluminum can act directly and/or indirectly in the pituitary, affecting ovarian steroidogenesis and altering the reproductive endocrine axis of mature O. niloticus females in an acute period of exposure.
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240,678
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240,841
Rapid genomic convergent evolution in experimental populations of Trinidadian guppies (Poecilia reticulata)
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240,842
Although rapid phenotypic evolution has been documented often, the genomic basis of rapid adaptation to natural environments is largely unknown in multicellular organisms. Population genomic studies of experimental populations of Trinidadian guppies (Poecilia reticulata) provide a unique opportunity to study this phenomenon. Guppy populations that were transplanted from high-predation (HP) to low-predation (LP) environments have been shown to evolve toward the phenotypes of naturally colonized LP populations in as few as eight generations. These changes persist in common garden experiments, indicating that they have a genetic basis. Here, we report results of whole genome variation in four experimental populations colonizing LP sites along with the corresponding HP source population. We examined genome-wide patterns of genetic variation to estimate past demography and used a combination of genome scans, forward simulations, and a novel analysis of allele frequency change vectors to uncover the signature of selection. We detected clear signals of population growth and bottlenecks at the genome-wide level that matched the known history of population numbers. We found a region on chromosome 15 under strong selection in three of the four populations and with our multivariate approach revealing subtle parallel changes in allele frequency in all four populations across this region. Investigating patterns of genome-wide selection in this uniquely replicated experiment offers remarkable insight into the mechanisms underlying rapid adaptation, providing a basis for comparison with other species and populations experiencing rapidly changing environments.
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241,282
How Cellular Agriculture Systems Can Promote Food Security
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241,283
Cellular agriculture, the manufacturing of animal-sourced foods by cell cultures, may promote food security by providing a food source that is available, accessible, utilized, and stable. The extent to which cellular agriculture can promote food security, however, will depend in part on the supply system by which it produces food. Many cellular agriculture companies appear poised to follow a centralized supply system, in which production is concentrated within a small number of large plants and products are distributed over a wide area. This model benefits from economies of scale, but has several weaknesses to food security. By being built of a handful of plants with products distributed by a large transportation network, the centralized model is vulnerable to closures, as became clear for animal-sourced centralized system during the COVID-19 pandemic. Cellular agriculture systems are being built now;therefore, alternative supply system models of decentralized and distributed systems should be considered as the systems of cellular agriculture production are established. This paper defines both the requirements of food security and three possible supply system models that cellular agriculture could take and evaluates each model based on the requirements of food security.
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Comprehensive pollution monitoring of the Egyptian Red Sea coast by using the environmental indicators.
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The environmental pollution indicators and multivariate statistical analysis were used to evaluate the potential ecological risk and the contamination of Fe, Mn, Zn, Ni, Pb, Cu, Cd, and Co in surface sediments of the Egyptian Red Sea coast. The results revealed that the studied area suffers from high contamination of certain metals such as the Hurghada area (Pb, Cd, Zn, Ni, and Cu), Quseir City area (Cd, Co, Pb, and Ni), and Safaga and Marsa Alam areas (Cd and Pb). Enrichment factor and principal components analysis reported that the pollution sources of Fe, Mn, and Co are related to natural weathering process while Cu, Zn, Ni, and Pb are related to anthropogenic sources as landfill, plastic rubbish, fishing boats, phosphate operations, and tourist activities. Moreover, Co and Cd metals can come from both of natural and anthropogenic sources. The average concentrations of Cd, Cu, Zn, Pb, Ni, and Co in sediments of the Egyptian Red Sea coast are higher than those in the coasts of the Red Sea (Saudi Arabia), Mediterranean Sea (Egypt and Libya), Bengal bay (India), and the Caspian Sea (Russia). However, the studied metal content is lower than the sediment quality guideline values except for Cd.
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241,348
Impacto Da Iatf (inseminação Artificial Em Tempo Fixo) Sobre Características De Importância Econômica Em Bovinos Nelore
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A produção de carne no Brasil cresce constantemente, assim como as exigências do mercado e dos consumidores, que fazem a pecuária de corte estar sempre em busca de estratégias tecnológicas e de manejo para garantir maior retorno econômico da atividade. Aliado ao melhoramento genético, o uso da inseminação artificial em tempo fixo (IATF) ganha destaque na produção comercial. O objetivo deste estudo foi comparar grupos de progênies oriundas de IATF e de monta natural (MN), a fim de avaliar o impacto da tecnologia reprodutiva no peso a desmama (PD), ganho de peso pós-desmama (GPD), perímetro escrotal (PE) e musculatura (MUS), além de verificar a idade ao primeiro parto (IPP) e tempo de permanência (TP) das fêmeas em rebanhos Nelore criados em sistema extensivo. Os dados avaliados foram provenientes de seis fazendas participantes do programa de melhoramento genético Nelore Qualitas, utilizou-se registro de 65.086 animais. Análises estatísticas dos dados foram realizadas pelo procedimento GLM e as médias ajustadas foram comparadas entre os grupos, G_MN (grupo de progênies oriundas de MN) e G_IA (grupo de progênies oriundas de inseminação artificial), a partir do teste de Tukey-Kramer no programa SAS. A concentração de nascimentos, em ambos os grupos, ocorreu entre agosto e novembro, período com maior oferta de pastagem, o que resultou maior potencial de desempenho das progênies ao desmame. Os touros apresentaram papel fundamental no desempenho das progênies, sendo aquelas oriundas de IA mais eficientes (p<0,001) nas características estudadas. Os fenótipos do grupo G_IA para PD, GPD, PE e MUS foram 187,02 kg, 81,69 kg, 24,09 cm e 3,92, respectivamente, e com valores genéticos iguais a 4,64 kg, 7,48 kg, 0,49 cm e 0,23, respectivamente. Estes resultados apresentaram fenótipos e valores genéticos superiores (p<0,001) aos do grupo G_MN, 180,8 kg, 69,81 kg, 23,68 cm e 3,78 e 0,04 kg, 0,05 kg, -0,01 cm e 0,02, respectivamente. A tendência genética para as características avaliadas apresentaram-se positivas em ambos os grupos, porém com superioridade ao grupo G_IA, indicando a importância do uso de biotecnologia reprodutiva nos rebanhos a fim de garantir aumento do mérito genético dos animais e maior retorno econômico da atividade para os produtores. Para IPP, as fêmeas mais precoces foram do grupo G_MN, sugerindo falta de correlação genética com as características presentes no índice de seleção e que o uso de IATF não interfere na precocidade. O contrário acontece com a característica TP, a qual apresentou maior resultado em fêmeas do grupo G_IA, sugerindo maior retorno econômico da atividade em rebanhos que utilizam biotecnologia reprodutiva. A rentabilidade econômica apresentou-se eficiente com a utilização de IATF nos rebanhos estudados.
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241,681
Efficacy of aggregation nano gel pheromone traps on the catchability of Rhynchophorus ferrugineus (Olivier) in Egypt
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Abstract Field experiments were carried out in the reclaimed land east of Cairo-Alexandria Desert Road, Village of Wardan, Giza Governorate, Egypt, to evaluate the impact of using nano-gels pheromone for the first time on the attraction of the RPW adults by pheromone traps compared to the control which is normal pheromone during two successive seasons in 2018 and 2019. Also, this study aimed to compare the efficacy of two types of trap, traditional trap (type 1) and dry funnel trap (type 2) in capturing of RPW adults. Results revealed that the nano gel pheromone traps attracted significantly more adults with an average of (4.26 and 3.56) adults/trap and total of (55.33 and 46.33) adults/trap compared to control with an average (2.69 and 2.46) and total of (35.00 and 32.00) adults/trap for the two seasons 2018 and 2019 respectively. Results indicated that the use of nano gel pheromone for the first time in field improved the trap catchability to RPW adults by 22.51 and 18.30% of total number of RPW adults captured throw out the two successive seasons respectively. In addition, there was no significant difference between the total numbers of RPW adults collected by the two tested type of traps over the two successive seasons. Furthermore, the sex ratio between males and females of total collected RPW adults found to be1: 1.43 and 1: 1.94 at 2018 and 2019 respectively.
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80,767
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241,891
The interactive effects of titanium dioxide nanoparticles and light on heterotrophic bacteria and microalgae associated with marine aggregates in nearshore waters
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Titanium dioxide nanoparticles (n-TiO2) are emerging contaminants and the ecological impact of these materials to the nearshore environment is largely unknown. The reactivity of n-TiO2 increases with light exposure, and the photocatalytic effects have been shown on cultures of bacteria and microalgae in the laboratory. The purpose of this study was to assess the response of natural bacterial and microalgal communities associated with marine aggregates to n-TiO2 under conditions similar to those found in the photic zone of nearshore waters. Nano and bulk TiO2 particles were incorporated into marine aggregates over 4 days under two light conditions: 6:18 and 0:24 (hours light:dark). The abundance and metabolic response of heterotrophic bacteria and viability of microalgae associated with aggregates were assessed. Although the proportion of living microalgae was unchanged, the abundance, total metabolic activity and functional diversity of heterotrophic bacteria were significantly altered by irradiated n-TiO2.
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Increasing the Awareness of Animal Welfare Science in Marine Mammal Conservation: Addressing Language, Translation and Reception Issues
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SIMPLE SUMMARY: Traditional conservation strategy focuses on population-level effects. However, the rapidly evolving scientific discipline of animal welfare science, in conjunction with growing societal expectations around the value of individuals, is prompting change in conservation. Despite growing recognition of this approach in terrestrial conservation efforts, limited application of animal welfare science to marine mammals has been observed. To investigate the factors underlying this disparity, we undertook an initial “Welfare in the Wild” workshop at the 32nd European Cetacean Society conference (La Spezia, Italy) to explore expert opinion on this topic. Secondly, we analysed the English language peer-reviewed literature to assess to what extent marine mammal welfare research is reported. The results of the workshop reveal a range of views about the scientific discipline of animal welfare science, with participants’ definitions varying depending on their disciplinary expertise. Meanwhile, the extensive literature review spanning 1950 to July 2020 revealed extremely low reporting of research related to welfare in the context of marine mammals, with only 0.96% (n = 299) of all published articles on marine mammal taxa (n = 31,221) featuring the word welfare in either the title, abstract or keywords. This highlighted a need to explore differences and distil common themes. Here we suggest practical solutions to the language, translation and reception issues of this cross-disciplinary collaboration between animal welfare science and marine mammal conservation. ABSTRACT: Integrating welfare principles into conservation strategy is an emerging synthesis that encourages consideration of individual animals’ quality of life in research, policies and law. However, these principles have gained limited traction in marine compared to terrestrial animal conservation. This manuscript investigates several factors that may be contributing to this disparity. In order to gauge current understanding of animal welfare science principles by marine mammal researchers and other stakeholders, a “Welfare in the Wild” workshop was convened at the 32nd European Cetacean Society conference (La Spezia, Italy, April 2018). The workshop was attended by 30 participants who completed pre- and post-workshop surveys on animal welfare principles. The survey results highlight a range of different views about exactly what animal welfare science is and how it can be applied to marine mammals. Specifically, participants’ definitions appeared to vary depending on the type of employment or research they engaged in, indicating a need for an interdisciplinary common language. Secondly, we analysed the peer-reviewed literature in order to ascertain where marine mammal publications exploring welfare were being published. From 1950 to July 2020, a total of 299 articles featured both marine mammal taxa (one or more) and the word welfare in the title, abstract or keywords. This represents just 0.96% of the total peer-reviewed published papers on marine mammal taxa (n = 31,221) during the same period. When examining articles published within “Welfare and Ethics” (n = 6133) and “Aquatic-focused” (n = 139,352) journals, just 1.2% (n = 71) and 0.04% (n = 57) of articles, respectively, featured the word welfare when examining marine mammals. With the aim of exploring how explicitly including welfare evaluations in marine mammal research and management can benefit conservation outcomes, we framed our workshop and quantitative literature review findings to provide practical solutions to the language, translation and reception issues of this burgeoning cross-disciplinary collaboration.
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