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In 2007 , a novel bunyavirus was found in Henan Province , China and named fever , thrombocytopenia and leukocytopenia syndrome virus ( FTLSV ) ; since then , FTLSV has been found in ticks and animals in many Chinese provinces . Human-to-human transmission has been documented , indicating that FTLSV should be considere...
FTLSV is novel bunyavirus belonging to genus Phlebovirus and was first found in Huaiyangshan area . The epidemiology and pathogenesis of FTLSV remain poorly understood . This lack of information underscores the importance of analyzing the movement and genetic history of FTLSV in China . Recent advances in Bayesian coal...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biology", "and", "life", "sciences", "microbiology", "virology" ]
2014
The Evolutionary History and Spatiotemporal Dynamics of the Fever, Thrombocytopenia and Leukocytopenia Syndrome Virus (FTLSV) in China
Vertebrate skin is characterized by its patterned array of appendages , whether feathers , hairs , or scales . In avian skin the distribution of feathers occurs on two distinct spatial levels . Grouping of feathers within discrete tracts , with bare skin lying between the tracts , is termed the macropattern , while the...
The distribution of hairs or feathers across the body is not homogeneous , and many animals have characteristic regions of their skin with either profuse or reduced coverage . These features , such as manes , crests , or bald patches , are seen in diverse species , suggesting that they can be selectively advantageous a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "systems", "biology", "developmental", "biology", "mathematics", "theoretical", "biology", "genetics", "biology", "evolutionary", "biology", "nonlinear", "dynamics", "genetics", "and", "genomics" ]
2011
Cryptic Patterning of Avian Skin Confers a Developmental Facility for Loss of Neck Feathering
Magnetoencephalography and electroencephalography ( MEG , EEG ) are essential techniques for studying distributed signal dynamics in the human brain . In particular , the functional role of neural oscillations remains to be clarified . For that reason , imaging methods need to identify distinct brain regions that concu...
The oscillatory activity of the brain produces a repertoire of signal dynamics that is rich and complex . Noninvasive recording techniques such as scalp magnetoencephalography and electroencephalography ( MEG , EEG ) are key methods to advance our comprehension of the role played by neural oscillations in brain functio...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "acoustics", "engineering", "and", "technology", "signal", "processing", "random", "variables", "neuroscience", "covariance", "signal", "filtering", "mathematics", "brain", "mapping", "discrete", "mathematics", "neuroimaging", "combinatorics", "research", "and", "analysis",...
2018
Imaging of neural oscillations with embedded inferential and group prevalence statistics
Urinary schistosomiasis , the result of infection by Schistosoma haematobium ( Sh ) , remains a major global health concern . A schistosome vaccine could represent a breakthrough in schistosomiasis control strategies , which are presently based on treatment with praziquantel ( PZQ ) . We report the safety and efficacy ...
Vaccines represent an attractive tool in the fight against schistosomiasis . Pre-clinical immunization studies with the schistosome enzyme 28 kDa glutathione S-transferase ( 28GST ) have shown a significant reduction of schistosome egg production and subsequent pathology . The objective of the present phase 3 trial was...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "schistosoma", "invertebrates", "medicine", "and", "health", "sciences", "immune", "physiology", "body", "fluids", "helminths", "immunology", "tropical", "diseases", "parasitic", "diseases", "animals", "vaccines", "preventive", "medicine", "urine", "neglected", "tropical"...
2018
Safety and efficacy of the rSh28GST urinary schistosomiasis vaccine: A phase 3 randomized, controlled trial in Senegalese children
Heterotrimeric G proteins act as the physical nexus between numerous receptors that respond to extracellular signals and proteins that drive the cytoplasmic response . The Gα subunit of the G protein , in particular , is highly constrained due to its many interactions with proteins that control or react to its conforma...
Proteins evolve new protein-protein interactions through changes to their residues . Many residue changes are harmful because they disrupt important existing interactions and functions . The more interactions a protein participates in , the more difficult it is to make changes that are not harmful to the protein . And ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry/molecular", "evolution", "computational", "biology/macromolecular", "structure", "analysis", "computational", "biology/comparative", "sequence", "analysis", "biochemistry/protein", "chemistry", "biochemistry/macromolecular", "chemistry" ]
2010
Evolution of a Signaling Nexus Constrained by Protein Interfaces and Conformational States
The occurrence of outbreaks of human rabies transmitted by Desmodus rotundus in Brazil in 2004 and 2005 reinforced the need for further research into this zoonosis . Studies of knowledge and practices related to the disease will help to define strategies for the avoidance of new cases , through the identification of ga...
In 2004 and 2005 , the occurrence of outbreaks of human rabies caused by the hematophagous bat , Desmodus rotundus in the Brazilian Amazon , highlighted the role of this bat in the transmission of the disease and the importance of further research on this zoonosis in this region . In the present study , we investigated...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "animal", "types", "medicine", "and", "health", "sciences", "education", "immunology", "tropical", "diseases", "sociology", "vertebrates", "social", "sciences", "domestic", "animals", "animals", "mammals", "preventive", "medicine", "rabies", "neglected", "tropical", "di...
2016
Rabies: Knowledge and Practices Regarding Rabies in Rural Communities of the Brazilian Amazon Basin
Lymphatic filariasis ( LF ) , a neglected tropical disease ( NTD ) preventable through mass drug administration ( MDA ) , is one of six diseases deemed possibly eradicable . Previously we developed one LF elimination scenario , which assumes MDA scale-up to continue in all countries that have previously undertaken MDA ...
Lymphatic filariasis ( LF ) is a neglected tropical disease ( NTD ) that is targeted for elimination and is thought to be potentially eradicable through once yearly mass drug administration ( MDA ) using drugs that are currently donated to LF endemic countries by the pharmaceutical companies that manufacture them . MDA...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "tropical", "diseases", "social", "sciences", "parasitic", "diseases", "animals", "data", "management", "filariasis", "pharmaceutics", "drug", "administration", "neglected", "tropical", "diseases", "lymphatic", "fi...
2017
How much will it cost to eradicate lymphatic filariasis? An analysis of the financial and economic costs of intensified efforts against lymphatic filariasis
Ever since we developed mitochondria to generate ATP , eukaryotes required intimate mito-nuclear communication . In addition , since reactive oxygen species are a cost of mitochondrial oxidative phosphorylation , this demands safeguards as protection from these harmful byproducts . Here we identified a critical transcr...
Energy homeostasis is a vital prerequisite for optimal nutrient utilization and prolonged survival in an environment with fluctuating and frequently scarce food resources . Numerous studies have elucidated the important roles of mitochondrial energy in fasting status but less is known about the role of mitochondria in ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
CAPER Is Vital for Energy and Redox Homeostasis by Integrating Glucose-Induced Mitochondrial Functions via ERR-α-Gabpa and Stress-Induced Adaptive Responses via NF-κB-cMYC
Dengue is a major global public health problem with increasing incidence and geographic spread . The epidemiology is complex with long inter-epidemic intervals and endemic with seasonal fluctuations . This study was initiated to investigate dengue transmission dynamics in Binh Thuan province , southern Vietnam . Wavele...
Dengue has become a major international public health problem due to increasing geographic distribution and a transition from epidemic transmission with long inter-epidemic intervals to endemic transmission with seasonal fluctuation . Seasonal and multi-annual cycles in dengue incidence vary over time and space . We pe...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "public", "health", "and", "epidemiology/epidemiology", "infectious", "diseases/neglected", "tropical", "diseases", "public", "health", "and", "epidemiology/infectious", "diseases", "infectious", "diseases/viral", "infections" ]
2010
Dengue Dynamics in Binh Thuan Province, Southern Vietnam: Periodicity, Synchronicity and Climate Variability
How duplicate genes provide genetic robustness remains an unresolved question . We have examined the duplicated histone deacetylases Sir2p and Hst1p in Saccharomyces cerevisiae and find that these paralogs with non-overlapping functions can provide genetic robustness against null mutations through a substitution mechan...
Gene duplication is an important force in evolution , as it provides a source of new genetic material . However , the mechanisms by which duplicated genes are retained and diverge are understudied at the experimental level . We have examined a pair of duplicated histone deacetylases Hst1p and Sir2p from baker's yeast ,...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "hst1p/sir2p", "genetic", "robustness", "duplication", "deacetylase", "repression", "molecular", "biology" ]
2007
Substitution as a Mechanism for Genetic Robustness: The Duplicated Deacetylases Hst1p and Sir2p in Saccharomyces cerevisiae
This study aimed to assess the impact of the Zika epidemic on the registration of birth defects in Brazil . We used an interrupted time series analysis design to identify changes in the trends in the registration of congenital anomalies . We obtained monthly data from Brazilian Live Birth Information System and used tw...
Zika can be characterized as one of the most significant emerging arboviruses . The Zika epidemic in Brazil and the subsequent increase in the number of serious brain anomalies , such as microcephaly , has demonstrated the importance of analysing the impact of Zika infection on the rate of congenital anomalies in an af...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "microcephaly", "pathology", "and", "laboratory", "medicine", "congenital", "anomalies", "statistics", "pathogens", "geographical", "locations", "microbiology", "time", "series", "analysis", "viruses", "developmental", "biology", "...
2019
Impact evaluation of Zika epidemic on congenital anomalies registration in Brazil: An interrupted time series analysis
More than 260 million people live with schistosomiasis and regular mass-treatment should be implemented to prevent morbidity . Praziquantel , dosed at 40 milligrams per kilogram bodyweight , is the drug of choice . During the last decades the WHO Tablet Pole–which estimates tablet need by height as representing weight–...
Schistosomiasis is an acute and chronic parasitic disease caused by Schistosoma worms , contributing to morbidity and mortality in 261 million people , mainly in poor , rural communities . In an effort to control schistosomiasis , the World Health Organization advocates for regular mass treatment to at-risk populations...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "children", "body", "weight", "medicine", "and", "health", "sciences", "body", "fluids", "education", "sociology", "tropical", "diseases", "geographical", "locations", "social", "sciences", "parasitic", "diseases", "health", "care", "urine", "age", "groups", "south", ...
2016
The Accuracy of Praziquantel Dose Poles for Mass Treatment of Schistosomiasis in School Girls in KwaZulu-Natal, South Africa
Gene duplication is a major mechanism facilitating adaptation to changing environments . From recent genomic analyses , the acquisition of zinc hypertolerance and hyperaccumulation characters discriminating Arabidopsis halleri from its zinc sensitive/non-accumulator closest relatives Arabidopsis lyrata and Arabidopsis ...
Arabidopsis halleri has developed the characters of zinc hypertolerance and hyperaccumulation as compared to its close relatives Arabidopsis thaliana and Arabidopsis lyrata . Different candidate genes were proposed to account for the appearance of these characters in A . halleri . One of them is MTP1 ( Metal Tolerance ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/comparative", "genomics", "genetics", "and", "genomics/functional", "genomics", "plant", "biology/plant-environment", "interactions", "genetics", "and", "genomics/plant", "genomes", "and", "evolution", "plant", "biology/plant", "biochemistry", "an...
2010
The Five AhMTP1 Zinc Transporters Undergo Different Evolutionary Fates towards Adaptive Evolution to Zinc Tolerance in Arabidopsis halleri
The ability of influenza A viruses ( IAVs ) to cross species barriers and evade host immunity is a major public health concern . Studies on the phylodynamics of IAVs across different scales – from the individual to the population – are essential for devising effective measures to predict , prevent or contain influenza ...
Influenza A viruses ( IAVs ) are major pathogens of humans and animals . Understanding how IAVs spread and evolve at different scales ( individual , regional , global ) in natural conditions is critical for preventing or managing influenza epidemics . A vast body of knowledge has been generated on the evolution of IAVs...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "public", "health", "and", "epidemiology", "emergence", "viral", "transmission", "and", "infection", "virology", "emerging", "viral", "diseases", "epidemiology", "infectious", "disease", "epidemiology", "biology", "evolutionary", "biology", "microbiology", "ev...
2012
Transmission of Equine Influenza Virus during an Outbreak Is Characterized by Frequent Mixed Infections and Loose Transmission Bottlenecks
N6-Methyladenosine ( m6A ) accounts for approximately 0 . 2% to 0 . 6% of all adenosine in mammalian mRNA , representing the most abundant internal mRNA modifications . m6A RNA immunoprecipitation followed by high-throughput sequencing ( MeRIP-seq ) is a powerful technique to map the m6A location transcriptome-wide . H...
N6-Methyladenosine ( m6A ) is one of the most abundant and conserved mRNA modifications . It has been reported to influence multiple steps of RNA life cycle and play an important role in the initiation and progression of human cancers . m6A RNA immunoprecipitation followed by high-throughput sequencing ( MeRIP-seq ) is...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cdna", "libraries", "sequencing", "techniques", "medicine", "and", "health", "sciences", "methods", "and", "resources", "gene", "regulation", "messenger", "rna", "cancers", "and", "neoplasms", "oncology", "immunoprecipitation", "forms", "of", "dna", "dna", "libraries...
2018
Refined RIP-seq protocol for epitranscriptome analysis with low input materials
Protozoa and bacteria infect various types of phagocytic cells including macrophages , monocytes , dendritic cells and eosinophils . However , it is not clear which of these cells process and present microbial antigens in vivo and in which cellular compartments parasite peptides are loaded onto Major Histocompatibility...
Phagocytosis is a cellular process that allows the engulfment of solid particles such as bacteria or parasites by the cell membrane and leads to the formation of an intracytoplasmic vesicle , the phagosome . Cell types that are capable of phagocytosis are called phagocytes and include monocytes , macrophages , dendriti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/antigen", "processing", "and", "recognition", "microbiology/immunity", "to", "infections", "microbiology/parasitology", "infectious", "diseases/protozoal", "infections", "immunology/immunity", "to", "infections" ]
2010
Direct Visualization of Peptide/MHC Complexes at the Surface and in the Intracellular Compartments of Cells Infected In Vivo by Leishmania major
Hepatitis C virus ( HCV ) p7 is a membrane-associated ion channel protein crucial for virus production . To analyze how p7 contributes to this process , we dissected HCV morphogenesis into sub-steps including recruitment of HCV core to lipid droplets ( LD ) , virus capsid assembly , unloading of core protein from LDs a...
Viroporins are small hydrophobic viral membrane proteins which oligomerize and modulate membrane properties to facilitate virus propagation . Within their membrane environment these proteins can form membrane pores or channels which change the permeability of membranes for ions . These properties are known to contribut...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "cell", "biology", "virology", "viral", "packaging", "biology", "microbiology", "viral", "replication" ]
2013
Hepatitis C Virus p7 is Critical for Capsid Assembly and Envelopment
Activity regulated neurotransmission shapes the computational properties of a neuron and involves the concerted action of many proteins . Classical , intuitive working models often assign specific proteins to specific steps in such complex cellular processes , whereas modern systems theories emphasize more integrated f...
Synapses , which are small structures where information is transmitted between neurons , are the functional units of computation in the brain implicated in information processing , learning and memory . In the last few years several genes that are expressed in synapses have been linked to brain disorders such as autism...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[ "algorithms", "systems", "biology", "computer", "science", "genomics", "computer", "modeling", "biology", "computational", "biology", "neuroscience", "genetics", "and", "genomics" ]
2012
Molecular Machines in the Synapse: Overlapping Protein Sets Control Distinct Steps in Neurosecretion
Rapid and reliable diagnostic assays for enteric ( typhoid and paratyphoid ) fever are urgently needed . We report the characterization of novel approach utilizing lymphocyte secretions , for diagnosing patients with enteric fever by the TPTest procedure . TPTest detects Salmonella-specific IgA responses in lymphocyte ...
Salmonella enterica serotype Typhi and Paratyphi A are responsible for typhoid and paratyphoid fever respectively and the disease caused is known generally as enteric fever . Appropriate and early diagnosis of the disease is important for initiation of treatment of the patient with a suitable antibiotic . The performan...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "immunology", "immune", "response", "biology" ]
2013
Evaluation of a Typhoid/Paratyphoid Diagnostic Assay (TPTest) Detecting Anti-Salmonella IgA in Secretions of Peripheral Blood Lymphocytes in Patients in Dhaka, Bangladesh
Type VI secretion systems ( T6SS ) are macromolecular machines of the cell envelope of Gram-negative bacteria responsible for bacterial killing and/or virulence towards different host cells . Here , we characterized the regulatory mechanism underlying expression of the enteroagregative Escherichia coli sci1 T6SS gene c...
DNA methylation plays an important role in the regulation of genes involved in assembly of cell surface adhesins or appendages . Methylation at a GATC motif by the Dam methylase influences binding of transcriptional regulators , leading to variation in the gene expression pattern . In several cases , this may lead to d...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacteriology", "macromolecular", "assemblies", "microbiology", "host-pathogen", "interaction", "dna", "transcription", "escherichia", "coli", "epigenetics", "bacterial", "pathogens", "gene", "expression", "microbial", "pathogens", "biology", "dna", "modification", "biochemis...
2011
An Epigenetic Switch Involving Overlapping Fur and DNA Methylation Optimizes Expression of a Type VI Secretion Gene Cluster
The 3’end genomic region encodes a wide range of regulatory process including mRNA stability , 3’ end processing and translation . Here , we systematically investigate the sequence determinants of 3’ end mediated expression control by measuring the effect of 13 , 000 designed 3’ end sequence variants on constitutive ex...
We present a large-scale experimental investigation into sequence determinants of 3’ end mediated gene expression regulation , by measuring 13 , 000 designed 3’ end sequences . While 3’ end sequences contribute to expression differences through a variety of mechanisms including mRNA stability and regulation of translat...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Systematic Dissection of the Sequence Determinants of Gene 3’ End Mediated Expression Control
The complex three-dimensional shapes of tree-like structures in biology are constrained by optimization principles , but the actual costs being minimized can be difficult to discern . We show that despite quite variable morphologies and functions , bifurcations in the scleractinian coral Madracis and in many different ...
Morphology is constrained by function and vice-versa . Often , intricate morphology can be explained by optimization of a cost . However , in biology , the exact form of the cost function is seldom clear . Previously , for many different natural trees authors have reported that most bifurcations are planar and we confi...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[ "medicine", "plant", "biology", "cardiovascular", "anatomy", "trees", "anatomy", "and", "physiology", "geometry", "neuroscience", "marine", "biology", "plant", "science", "mathematics", "computational", "neuroscience", "plants", "corals", "theoretical", "biology", "marine...
2012
Geometric Theory Predicts Bifurcations in Minimal Wiring Cost Trees in Biology Are Flat
Alternative splicing ( AS ) provides a potent mechanism for increasing protein diversity and modulating gene expression levels . How alternate splice sites are selected by the splicing machinery and how AS is integrated into gene regulation networks remain important questions of eukaryotic biology . Here we report that...
Mammalian gene expression is extensively controlled at the post-transcriptional level and understanding of the underlying mechanisms can provide important biomedical insights . Here we identified a number of novel alternate splicing ( AS ) events where the choice between competing splice sites ( ss ) is regulated by po...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "alternative", "splicing", "biochemistry", "rna", "developmental", "biology", "rna", "processing", "cell", "biology", "gene", "regulatory", "networks", "gene", "expression", "genetics", "gene", "regulation", "biology", "and", "life", "sciences", "cell", "differentiation...
2014
Regulation of mRNA Abundance by Polypyrimidine Tract-Binding Protein-Controlled Alternate 5′ Splice Site Choice
Acquired resistance is one of the major barriers to successful cancer therapy . The development of resistance is commonly attributed to genetic heterogeneity . However , heterogeneity of drug penetration of the tumor microenvironment both on the microscopic level within solid tumors as well as on the macroscopic level ...
Failure of cancer therapy is commonly attributed to the outgrowth of pre-existing resistant mutants already present prior to treatment , yet there is increasing evidence that the tumor microenvironment influences cell sensitivity to drugs and thus mediates the evolution of resistance during treatment . Here , we take i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Spatial Heterogeneity in Drug Concentrations Can Facilitate the Emergence of Resistance to Cancer Therapy
Uncontrolled immune responses to intracellular DNA have been shown to induce autoimmune diseases . Homeostasis regulation of immune responses to cytosolic DNA is critical for limiting the risk of autoimmunity and survival of the host . Here , we report that the E3 ubiquitin ligase tripartite motif protein 30α ( TRIM30α...
Negative-feedback regulation is a broad and pivotal biological event to maintain the homeostasis of the host . Viral DNA species derived from DNA viruses or retroviruses can activate STING signaling to produce pro-inflammatory cytokines and type I interferon , which further recruit immune cells or induce interferon sti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
TRIM30α Is a Negative-Feedback Regulator of the Intracellular DNA and DNA Virus-Triggered Response by Targeting STING
Selective and/or neutral processes may govern variation in DNA content and , ultimately , genome size . The observation in several organisms of a negative correlation between recombination rate and intron size could be compatible with a neutral model in which recombination is mutagenic for length changes . We used whol...
One major implication from genetic work done several decades ago is that the genome contains a lot of sequences that do not constitute genes or other functional elements . The total amount of DNA—the genome size—is thus not necessarily an indicator of DNA complexity or organismal complexity , an observation often refer...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genomics", "genetic", "mutation", "genome", "evolution", "genomic", "evolution", "genetics", "biology", "evolutionary", "biology", "comparative", "genomics", "evolutionary", "genetics", "genetics", "and", "genomics" ]
2012
Recombination Drives Vertebrate Genome Contraction
The fruit fly Drosophila melanogaster is a powerful model system for the study of innate immunity in vector insects as well as mammals . For vector insects , it is particularly important to understand all aspects of their antiviral immune defenses , which could eventually be harnessed to control the transmission of hum...
Mosquitoes and other biting insects transmit many harmful pathogens to humans , including parasites and viruses . In order to better protect humans from these diseases , we must gain a more complete understanding of how insects successfully—or unsuccessfully—combat these infections . While we know a great deal regardin...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "rna", "interference", "viral", "transmission", "and", "infection", "microbiology", "animals", "salmonellosis", "animal", "models", "bacterial", "diseases", "drosophila", "melanogaster", "model", "organisms", "expe...
2018
p38b and JAK-STAT signaling protect against Invertebrate iridescent virus 6 infection in Drosophila
RNA polymerase II ( RNAPII ) contains a unique C-terminal domain that is composed of heptapeptide repeats and which plays important regulatory roles during gene expression . RNAPII is responsible for the transcription of most protein-coding genes , a subset of non-coding genes , and retrotransposons . Retrotransposon t...
RNA Polymerase II ( RNAPII ) is the enzyme responsible for the transcription of all protein-coding genes , many non-coding genes and retrotransposons . RNAPII has a unique C-terminal domain ( CTD ) that is composed of repeats of a seven amino acid sequence and is conserved across species . The CTD functions as a recrui...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
The RNAPII-CTD Maintains Genome Integrity through Inhibition of Retrotransposon Gene Expression and Transposition
Homolog pairing , which plays a critical role in meiosis , poses a potential risk if it occurs in inappropriate tissues or between nonallelic sites , as it can lead to changes in gene expression , chromosome entanglements , and loss-of-heterozygosity due to mitotic recombination . This is particularly true in Drosophil...
Meiosis is a specialized cell division that permits the transmission of genetic material to following generations . A pivotal step for this process is the pairing and recombination between homologous chromosomes . In the case of Drosophila , which supports organismal-wide homolog pairing throughout development , it has...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Germline Progenitors Escape the Widespread Phenomenon of Homolog Pairing during Drosophila Development
The genetic basis of gene expression variation has long been studied with the aim to understand the landscape of regulatory variants , but also more recently to assist in the interpretation and elucidation of disease signals . To date , many studies have looked in specific tissues and population-based samples , but the...
Variation among individuals in the degree to which genes are expressed ( i . e . turned on or off ) is a characteristic exhibited by all species , and studies have identified regions of the genome harboring genetic variation affecting gene expression levels . To assess the degree of human inter-population variability i...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "genomics", "gene", "expression", "genetics", "population", "genetics", "biology", "human", "genetics", "genetics", "and", "genomics" ]
2012
Patterns of Cis Regulatory Variation in Diverse Human Populations
All living organisms exist in a precarious state of homeostasis that requires constant maintenance . A wide variety of stresses , including hypoxia , heat , and infection by pathogens perpetually threaten to imbalance this state . Organisms use a battery of defenses to mitigate damage and restore normal function . Prev...
It is increasingly clear that living organisms surveil their cellular structures and physiology to gain early warning about the presence and activity of pathogens or abiotic threats ( like hypoxia , hyper- or hypothermia , oxidative damage , etc . ) . Upon recognizing dysfunction , host defense networks are engaged to ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "rna", "interference", "pathology", "and", "laboratory", "medicine", "caenorhabditis", "gene", "regulation", "pathogens", "regulatory", "proteins", "immunology", "microbiology", "dna-binding", "proteins", "animals", ...
2017
A conserved mitochondrial surveillance pathway is required for defense against Pseudomonas aeruginosa
As emerging and re-emerging infectious arboviruses like dengue , chikungunya , and Zika threaten new populations worldwide , officials scramble to assess local severity and transmissibility , with little to no epidemiological history to draw upon . Indirect estimates of risk from vector habitat suitability maps are pro...
When novel infectious diseases like chikungunya or Zika emerge and threaten global spread , public health officials worldwide must assess the risk for local introductions and outbreaks . These assessments are made in anticipation of local case data , and officials must draw upon historic evidence from similar diseases ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "united", "states", "dengue", "virus", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "infectious", "disease", "epidemiology", "pathogens", "geographical", "locations", "microbiology", "north", "america", "viruses", "texas", "prob...
2019
Downgrading disease transmission risk estimates using terminal importations
Although scabies is estimated to be one of the most common skin conditions globally , prevalence data is not available in most settings . Disease mapping is required to develop and monitor successful control programs . Non-expert health workers are likely to play an important role in scabies mapping activities in endem...
Scabies is a parasitic infection that leads to significant morbidity worldwide . Mapping of scabies prevalence would improve the understanding of the true burden of disease and the need for control programs in specific countries and regions . The diagnosis of scabies in low resource settings , where the disease is most...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "solomon", "islands", "pruritus", "pathology", "and", "laboratory", "medicine", "education", "medical", "personnel", "sociology", "tropical", "diseases", "geographical", "locations", "social", "sciences", "parasitic", "diseases", ...
2019
The diagnosis of scabies by non-expert examiners: A study of diagnostic accuracy
Mutation of DNA damage checkpoint signaling kinases ataxia telangiectasia-mutated ( ATM ) or ATM- and Rad3-related ( ATR ) results in genomic instability disorders . However , it is not well understood how the instability observed in these syndromes relates to DNA replication/repair defects and failed checkpoint contro...
All cells have evolved pathways to maintain the integrity of the genetic information stored in their chromosomes . Endogenous and exogenous agents induce mutations and other damage in DNA , most frequently during DNA replication . Such DNA damage is under surveillance by a complex network of proteins that interact with...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "viral", "replication", "dna", "replication", "nucleic", "acids", "virology", "viral", "nucleic", "acid", "viruses", "and", "cancer", "dna", "biology", "dna", "recombination", "dna", "repair", "microbiology" ]
2013
ATM and ATR Activities Maintain Replication Fork Integrity during SV40 Chromatin Replication
The identity of plant host genetic factors controlling the composition of the plant microbiota and the extent to which plant genes affect associated microbial populations is currently unknown . Here , we use a candidate gene approach to investigate host effects on the phyllosphere community composition and abundance . ...
The leaves of plants are inhabited by a diverse community of microorganisms . These leaf inhabitants influence their hosts with respect to growth and resistance to abiotic and biotic stresses . Recent studies revealed that the bacterial communities associated with leaves undergo selection , resulting in conserved micro...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "prokaryotic", "models", "plant", "science", "model", "organisms", "plant", "and", "algal", "models", "plant", "microbiology", "genetic", "screens", "gene", "identification", "and", "analysis", "genetics", "synthetic", "biology", "biology", "and", "life", "sciences", ...
2014
A Synthetic Community Approach Reveals Plant Genotypes Affecting the Phyllosphere Microbiota
Hepatitis C virus ( HCV ) entry , translation , replication , and assembly occur with defined kinetics in distinct subcellular compartments . It is unclear how HCV spatially and temporally regulates these events within the host cell to coordinate its infection . We have developed a single molecule RNA detection assay t...
The stages of the viral life cycle are spatially and temporally regulated to coordinate the infectious process in a way that maximizes successful replication and spread . In this study , we used RNA in situ hybridization ( ISH ) to simultaneously detect HCV ( + ) and ( − ) RNAs and analyze the kinetics of HCV infection...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Spatiotemporal Analysis of Hepatitis C Virus Infection
While women are generally underrepresented in STEM fields , there are noticeable differences between fields . For instance , the gender ratio in biology is more balanced than in computer science . We were interested in how this difference is reflected in the interdisciplinary field of computational/quantitative biology...
There are fewer women than men working in Science , Technology , Engineering and Mathematics ( STEM ) . However , some fields within STEM are more gender-balanced than others . For instance , biology has a relatively high proportion of women , whereas there are few women in computer science . But what about computation...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "engineering", "and", "technology", "bibliometrics", "sexual", "and", "gender", "issues", "sociology", "social", "sciences", "social", "media", "scientists", "network", "analysis", "social", "networks", "social", "communication", "science", "and", "technology", "workfor...
2017
Women are underrepresented in computational biology: An analysis of the scholarly literature in biology, computer science and computational biology
Bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke Opisthorchis viverrini . This parasite is a major public health problem in different countries throughout the Greater Mekong sub-region ( Thailand , southern Vietnam , Lao PDR and Cambodia ) . Chron...
The control of snails involved in transmission of trematodes plays a key role in the fight against these parasitic diseases . While some snails have been studied thoroughly , only few genomic and proteomic studies have focused on Bithynia siamensis goniomphalos , the snail intermediate host for Opisthorchis viverrini ,...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "invertebrates", "medicine", "and", "health", "sciences", "immune", "cells", "immunology", "parasitic", "diseases", "animals", "protein", "expression", "gastropods", "peptide", "libraries", "molecular", "biology", "techniques", "snails", "research", "a...
2016
Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini
We propose a novel statistical framework for integrating the result from molecular quantitative trait loci ( QTL ) mapping into genome-wide genetic association analysis of complex traits , with the primary objectives of quantitatively assessing the enrichment of the molecular QTLs in complex trait-associated genetic va...
Genome-wide association studies ( GWAS ) have been tremendously successful in identifying genetic variants that impact complex diseases . However , the roles of such studies in disease etiology remain poorly understood , primarily because a large proportion of the GWAS findings are located in the non-coding region of t...
[ "Abstract", "Introduction", "Method", "Results", "Discussion" ]
[ "genome-wide", "association", "studies", "medicine", "and", "health", "sciences", "body", "fluids", "quantitative", "trait", "loci", "applied", "mathematics", "simulation", "and", "modeling", "algorithms", "mathematics", "genome", "analysis", "lipid", "analysis", "resea...
2017
Integrating molecular QTL data into genome-wide genetic association analysis: Probabilistic assessment of enrichment and colocalization
Correctly identifying nearest “neighbors” of a given microorganism is important in industrial and clinical applications where close relationships imply similar treatment . Microbial classification based on similarity of physiological and genetic organism traits ( polyphasic similarity ) is experimentally difficult and ...
Taxonomic classification of microorganisms according to similarity is important for industrial and clinical applications where close relationships imply similar uses and/or treatments . Current microbial taxonomy is phylogeny-guided , i . e . , the organisms are grouped based on their evolutionary relationships , defin...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Methods" ]
[]
2015
Functional Basis of Microorganism Classification
Chronic hepatitis C virus ( HCV ) infection is a leading cause of liver disease . Liver inflammation underlies infection-induced fibrosis , cirrhosis and liver cancer but the processes that promote hepatic inflammation by HCV are not defined . We provide a systems biology analysis with multiple lines of evidence to ind...
Hepatitis C virus ( HCV ) causes chronic infection of the liver and is a leading cause of liver inflammation , cirrhosis and liver cancer in nearly 200 million people worldwide . Importantly , hepatic inflammation during chronic HCV infection is considered to be the primary catalyst for progressive liver disease and de...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "immune", "cells", "cytokines", "immunology", "microbiology", "hepatitis", "hepatitis", "c", "infectious", "diseases", "inflammation", "viral", "immune", "evasion", "biology", "pathogenesis", "immune", "response", "immune", "system", "immunopathology", "monocy...
2013
IL-1β Production through the NLRP3 Inflammasome by Hepatic Macrophages Links Hepatitis C Virus Infection with Liver Inflammation and Disease
Misfolded proteins challenge the ability of cells to maintain protein homeostasis and can accumulate into toxic protein aggregates . As a consequence , cells have adopted a number of protein quality control pathways to prevent protein aggregation , promote protein folding , and target terminally misfolded proteins for ...
Most polypeptides by necessity must fold into three-dimensional structures in order to become functional proteins . Misfolding , either during or subsequent to initial folding , can result in toxic protein aggregation . As a consequence , cells have adopted a number of protein quality control pathways to prevent protei...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "flow", "cytometry", "engineering", "and", "technology", "enzymes", "enzymology", "light", "microscopy", "mutation", "microscopy", "materials", "science", "industrial", "engineering", "quality", "control", "chaperone", "proteins", "ligases", "research", "and", "analysis",...
2016
Prefoldin Promotes Proteasomal Degradation of Cytosolic Proteins with Missense Mutations by Maintaining Substrate Solubility
Strigolactones ( SLs ) are well known for their role in repressing shoot branching . In pea , increased transcript levels of SL biosynthesis genes are observed in stems of highly branched SL deficient ( ramosus1 ( rms1 ) and rms5 ) and SL response ( rms3 and rms4 ) mutants indicative of negative feedback control . In c...
Plant shoot branching results from the precise regulation of bud growth versus dormancy . Positive and negative feedback mechanisms are likely involved in the dynamic control of this highly plastic trait . Strigolactones , the most recently discovered class of plant hormones , play a key role in controlling shoot branc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "anatomy", "stem", "anatomy", "internodes", "brassica", "hormones", "plant", "science", "model", "organisms", "rice", "plant", "hormones", "membrane", "receptor", "signaling", "experimental", "organism", "systems", "hormone", "receptor", "signaling", "plants", ...
2017
The pea branching RMS2 gene encodes the PsAFB4/5 auxin receptor and is involved in an auxin-strigolactone regulation loop
Bacteria have developed various motility mechanisms to adapt to a variety of solid surfaces . A rhizosphere isolate , Paenibacillus sp . NAIST15-1 , exhibited unusual motility behavior . When spotted onto 1 . 5% agar media , Paenibacillus sp . formed many colonies , each of which moved around actively at a speed of 3 ....
Motility is crucial as it enables bacteria to seek out and colonize suitable environments . Here , a new isolate from the rhizosphere , Paenibacillus sp . , exhibited unusual motility behavior . When spotted on low wet , hard agar media , the bacterium formed many colonies , each of which moved around actively like an ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "bacteriology", "cell", "motility", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "bacillus", "microbiology", "light", "microscopy", "flagellar", "motility", "biomechanics", "biological", "locomotion", "prokaryotic", "...
2016
Genetic Analysis of Collective Motility of Paenibacillus sp. NAIST15-1
It is estimated that almost a half of all of people living in developing countries today are infected with roundworms , hookworms , or whipworms or combinations of these types of intestinal nematode worms . They can all be treated using safe , effective , and inexpensive single-dose generic drugs costing as little as U...
Almost one in every two people in the developing world is infected with one or more types of intestinal nematode worms . When fewer than 50% of people are infected , most carry only a few worms; but when more than 50% are infected , the number carrying moderate to heavy numbers increases markedly , as does the risk of ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "public", "health", "and", "epidemiology/infectious", "diseases" ]
2009
The Costs and Cost-Effectiveness of Mass Treatment for Intestinal Nematode Worm Infections Using Different Treatment Thresholds
Tetherin ( BST-2/CD317/HM1 . 24 ) is an IFN induced transmembrane protein that restricts release of a broad range of enveloped viruses . Important features required for Tetherin activity and regulation reside within the cytoplasmic domain . Here we demonstrate that two isoforms , derived by alternative translation init...
Regulation of innate immunity is critical to maintain a balance between control of a perceived threat and immunopathology . The interferon induced cellular factor Tetherin has been shown to restrict budding of a broad range of enveloped viruses including the human immunodeficiency virus . Though Tetherin appears to be ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "biology", "microbiology" ]
2012
Identification of Alternatively Translated Tetherin Isoforms with Differing Antiviral and Signaling Activities
Between 1998 and 2010 , S . Typhi was an uncommon cause of bloodstream infection ( BSI ) in Blantyre , Malawi and it was usually susceptible to first-line antimicrobial therapy . In 2011 an increase in a multidrug resistant ( MDR ) strain was detected through routine bacteriological surveillance conducted at Queen Eliz...
Typhoid fever is a major cause of disease and death around the world , particularly in resource limited settings , although reports suggest that until recently it has been much less prominent in sub-Saharan Africa ( SSA ) than Asia . Estimates of the precise burden of this disease are , however , difficult , as diagnos...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Rapid Emergence of Multidrug Resistant, H58-Lineage Salmonella Typhi in Blantyre, Malawi
Metabolomics is a powerful approach for discovering biomarkers and for characterizing the biochemical consequences of genetic variation . While untargeted metabolite profiling can measure thousands of signals in a single experiment , many biologically meaningful signals cannot be readily identified as known metabolites...
Untargeted metabolomics can systematically profile thousands of metabolite signals in biological samples and is an increasingly popular approach for discovering biomarkers and predictors for human traits and diseases . However , currently , a significant portion of the measured signals cannot be identified as known met...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genome-wide", "association", "studies", "statistics", "metaanalysis", "metabolomics", "mathematics", "metabolites", "genome", "analysis", "molecular", "biology", "techniques", "information", "technology", "data", "processing", "discrete", "mathematics", "combinatorics", "res...
2019
PAIRUP-MS: Pathway analysis and imputation to relate unknowns in profiles from mass spectrometry-based metabolite data
Barrett’s esophagus ( BE ) patients are routinely screened for high grade dysplasia ( HGD ) and esophageal adenocarcinoma ( EAC ) through endoscopic screening , during which multiple esophageal tissue samples are removed for histological analysis . We propose a computational method called the multistage clonal expansio...
Endoscopic screening for detecting cancer and cancer precursors in Barrett’s esophagus ( BE ) is currently informed by repeated systematic biopsying of the metaplastic BE tissue . Here we present a comprehensive multiscale model of the natural history of esophageal adenocarcinoma ( EAC ) , which describes the entire mu...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
A Multiscale Model Evaluates Screening for Neoplasia in Barrett’s Esophagus
Stage-specific transcription is a fundamental biological process in the life cycle of the Plasmodium parasite . Proteins containing the AP2 DNA-binding domain are responsible for stage-specific transcriptional regulation and belong to the only known family of transcription factors in Plasmodium parasites . Comprehensiv...
Although malarial parasites have a complex life cycle , they harbor only 30 transcription factors in their genome . The majority of these transcription factors belong to a single family referred to as the AP2 family . Our previous study suggested that stage-specific AP2 family transcription factors have critical roles ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Genome-Wide Identification of the Target Genes of AP2-O, a Plasmodium AP2-Family Transcription Factor
Having multiple domains in proteins can lead to partial folding and increased aggregation . Folding cooperativity , the all or nothing folding of a protein , can reduce this aggregation propensity . In agreement with bulk experiments , a coarse-grained structure-based model of the three-domain protein , E . coli Adenyl...
Most individual protein domains fold in an all or nothing fashion . This cooperative folding is important because it reduces the existence of partially folded proteins which can stick to each other and create disease causing aggregates . However , numerous proteins have multiple domains , independent units of folding ,...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "allosteric", "regulation", "biochemistry", "computational", "chemistry", "molecular", "dynamics", "proteins", "protein", "folding", "enzyme", "regulation", "protein", "structure", "protein", "domains", "biology", "and", "life", "sciences", "chemistry", "enzymology", "com...
2014
In the Multi-domain Protein Adenylate Kinase, Domain Insertion Facilitates Cooperative Folding while Accommodating Function at Domain Interfaces
Several intracellular Leishmania antigens have been identified in order to find a potential vaccine capable of conferring long lasting protection against Leishmania infection . Histones and Acid Ribosomal proteins are already known to induce an effective immune response and have successfully been tested in the cutaneou...
Visceral leishmaniasis caused by Leishmania infantum is the most severe form of leishmaniasis . The disease is fatal if not treated and there is no vaccine available for human use . In the search for potential antigens , the protective ability of conserved parasite protein families such as L . infantum histones ( HIS )...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Vaccination with Leishmania infantum Acidic Ribosomal P0 but Not with Nucleosomal Histones Proteins Controls Leishmania infantum Infection in Hamsters
Lymphatic filariasis ( LF ) , onchocerciasis , schistosomiasis , soil-transmitted helminths ( STH ) and trachoma represent the five most prevalent neglected tropical diseases ( NTDs ) . They can be controlled or eliminated by means of safe and cost-effective interventions delivered through programs of Mass Drug Adminis...
The five most prevalent neglected tropical diseases ( NTDs ) are lymphatic filariasis ( LF ) , onchocerciasis , schistosomiasis , soil-transmitted helminths ( STH ) and trachoma . They can be controlled or eliminated by means of safe and cost-effective interventions delivered through programs of Mass Drug Administratio...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "tropical", "diseases", "social", "sciences", "parasitic", "diseases", "anemia", "health", "care", "bacterial", "diseases", "eye", "diseases", "neglected", "tropical", "diseases", "onchocerciasis", "public", "and", "occupational"...
2017
The Socioeconomic Benefit to Individuals of Achieving the 2020 Targets for Five Preventive Chemotherapy Neglected Tropical Diseases
The behaviour of insect vectors has an important bearing on the epidemiology of the diseases they transmit , and on the opportunities for vector control . Two sorts of electrocuting device have been particularly useful for studying the behaviour of tsetse flies ( Glossina spp ) , the vectors of the trypanosomes that ca...
With a view to refining studies of tsetse fly behaviour , we used a catch method , in the field in Zimbabwe , to assess and improve the efficiency of 0 . 5m2 electrocuting grids commonly used to sample tsetse that collide with netting while in flight or which alight on cloth . We found that the efficiency of both sorts...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Efficacy of Electrocuting Devices to Catch Tsetse Flies (Glossinidae) and Other Diptera
Phages shape the structure of natural bacterial communities and can be effective therapeutic agents . Bacterial resistance to phage infection , however , limits the usefulness of phage therapies and could destabilise community structures , especially if individual resistance mutations provide cross-resistance against m...
Phage therapy is a promising alternative to antibiotics for treating bacterial infections . Yet as with antibiotics , bacteria readily evolve resistance to phage attack , including cross-resistance that protects against multiple phages at once and so limits the usefulness of phage cocktails . Here we show , using labor...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "bacteriology", "organismal", "evolution", "microbial", "mutation", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "bacteriophages", "pathogens", "microbiology", "pili", "and", "fimbriae", "pseudomonas", "aeruginosa", "viruses", "de...
2018
Cross-resistance is modular in bacteria–phage interactions
Keratocytes are fast-moving cells in which adhesion dynamics are tightly coupled to the actin polymerization motor that drives migration , resulting in highly coordinated cell movement . We have found that modifying the adhesive properties of the underlying substrate has a dramatic effect on keratocyte morphology . Cel...
Cell migration is important for many biological processes: white blood cells chase down and kill bacteria to guard against infection , epithelial cells crawl across open wounds to promote healing , and embryonic cells move collectively to form organs and tissues during embryogenesis . In all of these cases , migration ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "systems", "biology", "cell", "motility", "molecular", "cell", "biology", "biology", "biophysics", "genetics", "and", "genomics" ]
2011
An Adhesion-Dependent Switch between Mechanisms That Determine Motile Cell Shape
Phosphorothioate ( PT ) modifications of the DNA backbone , widespread in prokaryotes , are first identified in bacterial enteropathogens Escherichia coli B7A more than a decade ago . However , methods for high resolution mapping of PT modification level are still lacking . Here , we developed the PT-IC-seq technique ,...
Phosphorothioate ( PT ) modification is a novel DNA modification , previous studies showed that PT modifications in E . coli occure at GpsAAC/GpsTTC motifs , but the modification frequency at each site are not known . In this study , we introduced two methods: PT-IC-seq , which could quantitatively characterize the gen...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "bacteriology", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "microbiology", "organisms", "bacterial", "diseases", "enterobacteriaceae", "genome", "analysis", "bacterial", "genetics", "sequence", "motif", "analysis", ...
2019
Quantitative mapping of DNA phosphorothioatome reveals phosphorothioate heterogeneity of low modification frequency
One of the central questions of developmental biology is how cells of equivalent potential—an equivalence group—come to adopt specific cellular fates . In this study we have used a combination of live imaging , single cell lineage analyses , and perturbation of specific signaling pathways to dissect the specification o...
How specific genes and signals act on initially identical cells to generate the different tissues of the body remains one of the central questions of developmental genetics . Zebrafish are a useful model system to tackle this question as the optically clear embryo allows direct imaging of forming tissues , tracking ind...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "embryology", "animal", "genetics", "genetics", "molecular", "genetics", "biology", "morphogenesis", "pattern", "formation", "genetics", "and", "genomics", "cell", "fate", "determination", "gene", "function" ]
2012
Morphogenesis and Cell Fate Determination within the Adaxial Cell Equivalence Group of the Zebrafish Myotome
Rabies virus ( RABV ) invades the central nervous system and nearly always causes fatal disease in humans . How RABV interacts with host neuron membrane receptors to become internalized and cause rabid symptoms is not yet fully understood . Here , we identified a novel receptor of RABV , which RABV uses to infect neuro...
Rabies virus ( RABV ) invades the central nervous system and nearly always causes fatal disease in humans . How RABV interacts with host neuron membrane receptors to become internalized and cause rabid symptoms is not yet fully understood . Here , we identified metabotropic glutamate receptor subtype 2 ( mGluR2 ) as a ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "immune", "physiology", "vesicular", "stomatitis", "virus", "pathology", "and", "laboratory", "medicine", "vesicles", "nervous", "system", "gene", "regulation", "pathogens", "immunology", "microbiology", "neuroscience", "viruses", ...
2018
Metabotropic glutamate receptor subtype 2 is a cellular receptor for rabies virus
During postnatal life the cerebral cortex passes through critical periods of plasticity allowing its physiological adaptation to the environment . In the visual cortex , critical period onset and closure are influenced by the non-cell autonomous activity of the Otx2 homeoprotein transcription factor , which regulates t...
Classically , cell signaling is based on the secretion of molecules that bind cell surface receptors . Lipophilic agents can do without cell-surface receptors due to their ability to diffuse through the plasma membrane , but this is normally not the case for proteins , which cannot pass the membrane barrier . However ,...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "nervous", "system", "gene", "regulation", "regulatory", "proteins", "brain", "dna-binding", "proteins", "social", "sciences", "neuroscience", "visual", "acuity", "physiological", "processes", "transcription", "...
2016
A Mouse Model for Conditional Secretion of Specific Single-Chain Antibodies Provides Genetic Evidence for Regulation of Cortical Plasticity by a Non-cell Autonomous Homeoprotein Transcription Factor
Participants in mass gathering events are at risk of acquiring imported and locally endemic infectious diseases . The 2014 dengue outbreak in Tokyo gathered attention since it was the first time in 70 years for Japan to experience an autochthonous transmission . Preparation for emerging infectious threats is essential ...
Dengue is a mosquito-borne disease that is most prevalent among the emerging arboviruses . Most patients recover from dengue without complications , but a small portion of cases may progress to severe dengue which carries a high mortality rate if left untreated . In 2014 , a dengue outbreak unexpectedly occurred in Tok...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "physicians", "medical", "doctors", "medical", "personnel", "japan", "sociology", "geographical", "locations", "tropical", "diseases", "social", "sciences", "vector-borne", "diseases", "health", "care", "health", "care", "provide...
2018
Health preparedness plan for dengue detection during the 2020 summer Olympic and Paralympic games in Tokyo
Studies of learning mechanisms critically depend on the ability to accurately assess learning outcomes . This assessment can be impeded by the often complex , multidimensional nature of behavior . We present a novel , automated approach to evaluating imitative learning . Conceptually , our approach estimates how much o...
Measuring learning outcomes is a critical objective of research into the mechanisms that support learning . Demonstration that a given manipulation results in better or worse learning outcomes requires an accurate and consistent measurement of learning quality . However , many behaviors ( e . g . speaking , walking , a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "syllables", "learning", "ornithology", "linguistics", "vocalization", "vertebrates", "social", "sciences", "neuroscience", "animals", "learning", "and", "memory", "animal", "signaling", "and", "communication", "cognitive", "psychology", "animal", "behavior", "mathematics",...
2018
An automated approach to the quantitation of vocalizations and vocal learning in the songbird
Pathogenic uncultivable treponemes comprise human and animal pathogens including agents of syphilis , yaws , bejel , pinta , and venereal spirochetosis in rabbits and hares . A set of 10 treponemal genome sequences including those of 4 Treponema pallidum ssp . pallidum ( TPA ) strains ( Nichols , DAL-1 , Mexico A , SS1...
The genus Treponema comprises several uncultivable human and animal pathogens including Treponema pallidum ssp . pallidum ( TPA ) , the causative agent of syphilis , T . p . ssp . pertenue ( TPE , the causative agent of yaws ) , and T . p . ssp . endemicum ( TEN , the causative agent of bejel ) . Simian TPE strain Frib...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions
The major histocompatibility complex ( MHC ) is a crucial component of the vertebrate immune system and shows extremely high levels of genetic polymorphism . The extraordinary genetic variation is thought to be ancient polymorphisms maintained by balancing selection . However , introgression from related species was re...
The major histocompatibility complex ( MHC ) , a crucial component of the defense against pathogens , contains the most polymorphic functional genes in vertebrate genomes . The extraordinary genetic variation is generally considered to be ancient . We investigated whether a previously neglected mechanism , introgressio...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[ "haplotypes", "major", "histocompatibility", "complex", "genetic", "polymorphism", "genome", "evolution", "clinical", "immunology", "natural", "selection", "genetics", "introgression", "biology", "and", "life", "sciences", "population", "genetics", "gene", "flow", "comput...
2014
Introgression from Domestic Goat Generated Variation at the Major Histocompatibility Complex of Alpine Ibex
The importance of microRNAs in the regulation of various aspects of biology and disease is well recognized . However , what remains largely unappreciated is that a significant number of miRNAs are embedded within and are often co-expressed with protein-coding host genes . Such a configuration raises the possibility of ...
Animal genomes encode hundreds of microRNA genes that impact all areas of biology by limiting the expression of their targets . What remains largely unappreciated is that a significant proportion of microRNA genes are embedded within protein-coding genes , and are often co-expressed with their hosts , which raises the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "death", "invertebrates", "animal", "genetics", "gene", "regulation", "cell", "cycle", "and", "cell", "division", "cell", "processes", "animals", "gene", "function", "animal", "models", "drosophila", "melanogaster", "model", "organisms", "molecular", "genetics...
2014
An Intronic microRNA Links Rb/E2F and EGFR Signaling
Yaws is a neglected tropical disease , caused by Treponema pallidum subsp . pertenue . The disease causes chronic lesions , primarily in young children living in remote villages in tropical climates . As part of a global yaws eradication campaign initiated by the World Health Organization , we sought to develop and eva...
Yaws is a neglected treponemal infection that is often transmitted among children in developing countries . Eradication programs in the 1940–50’s significantly reduced the incidence of yaws , but the disease has resurged . The World Health Organization has proposed to eliminate yaws by 2020 , and mass treatment trials ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "geomorphology", "taxonomy", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "landforms", "pathogens", "topography", "tropical", "diseases", "microbiology", "treponematoses", "bacterial", "diseases", "phylogenetics", "data", "managemen...
2017
Development of a Multilocus Sequence Typing (MLST) scheme for Treponema pallidum subsp. pertenue: Application to yaws in Lihir Island, Papua New Guinea
Neuronal representation and extraction of spatial information are essential for behavioral control . For flying insects , a plausible way to gain spatial information is to exploit distance-dependent optic flow that is generated during translational self-motion . Optic flow is computed by arrays of local motion detector...
Insects , with their limited brain resources and high performance in a wide behavioral repertoire , are exquisite model systems for studying parsimonious signal processing . They extract spatial information by actively shaping their self-motion ( e . g . when performing peering movements or during flight segments with ...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[ "velocity", "medicine", "and", "health", "sciences", "classical", "mechanics", "engineering", "and", "technology", "motion", "detectors", "social", "sciences", "neuroscience", "insect", "flight", "biological", "locomotion", "flight", "(biology)", "vision", "sensory", "p...
2017
Local motion adaptation enhances the representation of spatial structure at EMD arrays
The role of IL-1β and IL-18 during lung infection with the gram-negative bacterium Francisella tularensis LVS has not been characterized in detail . Here , using a mouse model of pneumonic tularemia , we show that both cytokines are protective , but through different mechanisms . Il-18-/- mice quickly succumb to the in...
Francisella tularensis is a Gram-negative bacterium that infects macrophages and other cell types causing tularemia . F . tularensis is considered a potential bioterrorism agent and is a prime model intracellular bacterium to study the interaction of pathogens with the host immune system . The role of the proinflammato...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Production of Anti-LPS IgM by B1a B Cells Depends on IL-1β and Is Protective against Lung Infection with Francisella tularensis LVS
How tightly packed chromatin is thoroughly inspected for DNA damage is one of the fundamental unanswered questions in biology . In particular , the effective excision of carcinogenic lesions caused by the ultraviolet ( UV ) radiation of sunlight depends on UV-damaged DNA-binding protein ( UV-DDB ) , but the mechanism b...
Like all molecules in living organisms , DNA undergoes spontaneous decay and is constantly under attack by endogenous and environmental agents . Unlike other molecules , however , DNA—the blueprint of heredity—cannot be re-created de novo; it can only be copied . The original blueprint must therefore remain pristine . ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology", "medicine", "dermatology", "public", "health", "and", "epidemiology", "biology", "geriatrics", "molecular", "cell", "biology", "public", "health" ]
2011
Regulation of Nucleotide Excision Repair by UV-DDB: Prioritization of Damage Recognition to Internucleosomal DNA
Breast cancer , the second leading cause of cancer death of women worldwide , is a heterogenous disease with multiple different subtypes . These subtypes carry important implications for prognosis and therapy . Interestingly , it is known that these different subtypes not only have different biological behaviors , but ...
Breast cancer , the second leading cause of cancer death of women worldwide , is a heterogenous disease . Different subtypes of breast cancer display very different expression programs , and these expression programs are associated with different patient outcomes and with different treatment protocols . However , littl...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "breast", "tumors", "gene", "regulation", "cancers", "and", "neoplasms", "oncology", "alternative", "splicing", "genome", "analysis", "bioassays", "and", "physiological", "analysis", "research", "and", "analysis", "methods", "s...
2017
Robust stratification of breast cancer subtypes using differential patterns of transcript isoform expression
Whole-genome sequencing is a powerful technique for obtaining the reference sequence information of multiple organisms . Its use can be dramatically expanded to rapidly identify genomic variations , which can be linked with phenotypes to obtain biological insights . We explored these potential applications using the em...
In this manuscript , we describe novel applications of the newly developed Solexa sequencing technology . We aim to provide insights into the following questions: ( 1 ) Can whole-genome sequencing , while rapidly surveying mega-bases of genome information , also reliably identify variations at the base-pair resolution ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genomics", "genetics", "and", "genomics/microbial", "evolution", "and", "genomics", "microbiology/microbial", "physiology", "and", "metabolism" ]
2008
High-Precision, Whole-Genome Sequencing of Laboratory Strains Facilitates Genetic Studies
Pools of mutants of minimal complexity but maximal coverage of genes of interest facilitate screening for genes under selection in a particular environment . We constructed individual deletion mutants in 1 , 023 Salmonella enterica serovar Typhimurium genes , including almost all genes found in Salmonella but not in re...
One strategy to define bacterial genes with a role during infection involves the screening of a pool of random transposon insertion mutants , where each mutant is identifiable by a specific motif or unique transcript . Changes in the survival of each mutant indicate a role of the mutated region during infection . To en...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "genetics", "and", "genomics/functional", "genomics", "cell", "biology/microbial", "growth", "and", "development", "genetics", "and", "genomics/disease", "models", "genetics", "and", "genomics/gene", "function", "genetics", "and", "genomics/genetics", "of",...
2009
Analysis of Pools of Targeted Salmonella Deletion Mutants Identifies Novel Genes Affecting Fitness during Competitive Infection in Mice
Resting CD4+ T-cells harboring inducible HIV proviruses are a critical reservoir in antiretroviral therapy ( ART ) -treated subjects . These cells express little to no viral protein , and thus neither die by viral cytopathic effects , nor are efficiently cleared by immune effectors . Elimination of this reservoir is th...
Although modern therapies have greatly improved the lives of HIV-positive people with access to care , a cure remains elusive . This leaves these individuals burdened by a lifelong commitment to medication , and fails to fully restore health . Curing infection would likely require therapies that combine the ability to ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "flow", "cytometry", "hiv", "infections", "medicine", "and", "health", "sciences", "immune", "cells", "enzyme-linked", "immunoassays", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "microbiology", "cloning", "retroviruses", "...
2016
A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes
Apoptotic cell death is an important response to genotoxic stress that prevents oncogenesis . It is known that tissues can differ in their apoptotic response , but molecular mechanisms are little understood . Here , we show that Drosophila polyploid endocycling cells ( G/S cycle ) repress the apoptotic response to DNA ...
In order to maintain genome integrity , eukaryotic cells have evolved multiple ways to respond to DNA damage stress . One of the major cellular responses is apoptosis , during which the cell undergoes programmed cell death in order to prevent the propagation of the damaged genome to daughter cells . Although clinical o...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "and", "life", "sciences" ]
2014
Low Levels of p53 Protein and Chromatin Silencing of p53 Target Genes Repress Apoptosis in Drosophila Endocycling Cells
Microbial infections usually lead to host innate immune responses and inflammation . These responses most often limit pathogen replication although they can also result in host-tissue damage . The enteropathogenic bacteria Salmonella Typhimurium utilizes a type III secretion system to induce intestinal inflammation by ...
The inflammatory response to microbial pathogens usually limits their replication but it can also cause tissue damage . The enteropathogenic bacteria Salmonella Typhimurium stimulate host signal transduction pathways that result in inflammation . We show here that a family of related Salmonella Typhimurium effector pro...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "transfection", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "physiology", "cytokines", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "regulatory", "proteins", "immunology", "microbiology", "dna-bindin...
2016
A Family of Salmonella Type III Secretion Effector Proteins Selectively Targets the NF-κB Signaling Pathway to Preserve Host Homeostasis
African trypanosomosis , primarily transmitted by tsetse flies , remains a serious public health and economic challenge in sub-Saharan Africa . Interventions employing natural repellents from non-preferred hosts of tsetse flies represent a promising management approach . Although zebras have been identified as non-pref...
The use of repellents from non-preferred hosts represents an innovative approach to control animal African trypanosomosis by limiting contact between tsetse flies and livestock . Although zebras are non-preferred hosts , the possible chemical basis of their avoidance by tsetse flies is unknown . We hypothesized that ce...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "body", "fluids", "chemical", "compounds", "ketones", "vertebrates", "animals", "mammals", "parasitic", "protozoans", "organic", "compounds", "glossina", "parasitology", "urine", "protozoans", "tsetse", "fly", "i...
2019
Zebra skin odor repels the savannah tsetse fly, Glossina pallidipes (Diptera: Glossinidae)
A close relative of poliovirus , enterovirus 71 ( EV71 ) is regarded as an important neurotropic virus of serious public health concern . EV71 causes Hand , Foot and Mouth Disease and has been associated with neurological complications in young children . Our limited understanding of the mechanisms involved in its neur...
A close relative of poliovirus , Enterovirus 71 ( EV71 ) causes Hand , Foot and Mouth Disease and has been associated with neurological complications in young children . The lack of effective therapeutics is largely due to our limited understanding of the mechanisms involved in EV71 neuropathogenesis . Here , using a p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "transfection", "medicine", "and", "health", "sciences", "immune", "physiology", "nervous", "system", "gene", "regulation", "immunology", "microbiology", "neuroscience", "immunoprecipitation", "mitochondria", "co-immunoprecipitation", "bioenergetics", "molecular", "biology", ...
2018
Prohibitin plays a critical role in Enterovirus 71 neuropathogenesis
Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis , a health-threatening human systemic mycosis endemic to Latin America . Infection by Paracoccidioides , a dimorphic fungus in the order Onygenales , is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a ...
Paracoccidioides sp . are fungal pathogens that cause paracoccidioidomycosis in humans . They are part of a larger group of dimorphic fungi causing pulmonary infections in immunocompetent people , whereas many other fungi cause opportunistic infections . We sequenced the genomes of two strains of Paracoccidioides brasi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome", "evolution", "microbiology", "fungal", "physiology", "genome", "sequencing", "fungal", "evolution", "microbial", "evolution", "genome", "complexity", "mycology", "microbial", "pathogens", "comparative", "genomics", "biology", "pathogenesis", "genomics", "computati...
2011
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
Microbial infection during various stages of human development produces widely different clinical outcomes , yet the links between age-related changes in the immune compartment and functional immunity remain unclear . The ability of the immune system to respond to specific antigens and mediate protection in early life ...
Newborns typically have a heightened sensitivity to infectious diseases , the reasons for which are not yet well understood . One contributing factor is the limited diversity of lymphocyte receptors early in life to recognize antigen and control infection . We have previously shown that antigen-specific CD8+ T cell rep...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "models", "of", "infection", "viral", "vaccines", "adaptive", "immunity", "immunity", "virology", "biology", "microbiology" ]
2013
Acute Neonatal Infections ‘Lock-In’ a Suboptimal CD8+ T Cell Repertoire with Impaired Recall Responses
Small oligomers formed early in the process of amyloid fibril formation may be the major toxic species in Alzheimer's disease . We investigate the early stages of amyloid aggregation for the tau fragment AcPHF6 ( Ac-VQIVYK-NH2 ) using an implicit solvent all-atom model and extensive Monte Carlo simulations of 12 , 24 ,...
It is believed that the self association of certain protein molecules into aggregated structures , known as amyloid fibrils , plays an important role in a variety of human diseases , such as Alzheimer's disease and Parkinson's disease . Although the ability to form such amyloid fibrils is a common property for proteins...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biophysics/theory", "and", "simulation" ]
2008
Formation and Growth of Oligomers: A Monte Carlo Study of an Amyloid Tau Fragment
Preventive chemotherapy has long been practiced against nematode parasites of livestock , leading to widespread drug resistance , and is increasingly being adopted for eradication of human parasitic nematodes even though it is similarly likely to lead to drug resistance . Given that the genetic architecture of resistan...
Teladorsagia circumcincta is an economically significant nematode ( roundworm ) pathogen affecting sheep and goats in temperate regions of the world . The widespread use of prophylactic treatment has resulted in rapid selection for anthelmintic ( anti-worm drug ) resistance in this and other species of livestock parasi...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "ruminants", "population", "genetics", "vertebrates", "animals", "mammals", "invertebrate", "genomics", "genetic", "mapping", "molecular", "biology", "techniques", "population", "biology", "research", "and", "analysis", "methods", "gene", "mapping", "introgression", "mole...
2017
Genomic introgression mapping of field-derived multiple-anthelmintic resistance in Teladorsagia circumcincta
G-protein–coupled receptors ( GPCRs ) are generally thought to signal to second messengers like cyclic AMP ( cAMP ) from the cell surface and to become internalized upon repeated or prolonged stimulation . Once internalized , they are supposed to stop signaling to second messengers but may trigger nonclassical signals ...
Cells respond to many environmental cues through the activity of cell surface receptor proteins , which sense these cues and convey that information to signaling molecules inside the cell . G-protein–coupled receptors ( GPCRs ) form the largest eukaryotic family of plasma membrane receptors . They convert the informati...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "diabetes", "and", "endocrinology/thyroid", "cell", "biology/cell", "signaling" ]
2009
Persistent cAMP-Signals Triggered by Internalized G-Protein–Coupled Receptors
Recent increased activity of the mosquito-borne Murray Valley encephalitis virus ( MVEV ) in Australia has renewed concerns regarding its potential to spread and cause disease . To better understand the genetic relationships between earlier and more recent circulating strains , patterns of virus movement , as well as t...
Murray Valley encephalitis virus is the most significant cause of mosquito-borne encephalitis in humans in Australia , and can also cause neurological disease in horses . This study reports an expanded phylogenetic study of this virus and the first molecular evolutionary analysis . Of the four recognized genotypes of M...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
The Molecular Epidemiology and Evolution of Murray Valley Encephalitis Virus: Recent Emergence of Distinct Sub-lineages of the Dominant Genotype 1
The maintenance of chromosome integrity is crucial for genetic stability . However , programmed chromosome fragmentations are known to occur in many organisms , and in the ciliate Tetrahymena the five germline chromosomes are fragmented into hundreds of minichromosomes during somatic nuclear differentiation . Here , we...
Chromatin diminution was first discovered in the parasite Parascaris univalens by T . Boveri more than one century ago . This process eliminates a large proportion of the genome accompanying germline-soma differentiation during development . Ciliates also carry out DNA elimination that have been studied in depth . Duri...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "Methods" ]
[ "sequencing", "techniques", "chromosome", "structure", "and", "function", "genomic", "databases", "telomeres", "model", "organisms", "protozoans", "genome", "analysis", "tetrahymena", "thermophila", "sequence", "motif", "analysis", "ciliate", "protozoans", "molecular", "b...
2016
Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila
Cell migration in 3D microenvironments is fundamental to development , homeostasis and the pathobiology of diseases such as cancer . Rab-coupling protein ( RCP ) dependent co-trafficking of α5β1 and EGFR1 promotes cancer cell invasion into fibronectin ( FN ) containing extracellular matrix ( ECM ) , by potentiating EGF...
The majority of cancer-related fatalities are caused by the movement of cancer cells away from the primary site to form metastases , making understanding the signalling mechanisms which underpin cell migration and invasion through their local environment of paramount importance . Much has been discovered about key even...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "cell", "motility", "cancer", "cell", "migration", "gene", "regulation", "enzymes", "enzymology", "signaling", "networks", "fluorophotometry", "developmental", "biology", "gtpase", "signaling", "network", "analysis", "research", "and", "analysis", "methods", "small", "i...
2016
A MAPK-Driven Feedback Loop Suppresses Rac Activity to Promote RhoA-Driven Cancer Cell Invasion
Forward genetic screens in model organisms are an attractive means to identify those genes involved in any complex biological process , including neural circuit assembly . Although mutagenesis screens are readily performed to saturation , gene identification rarely is , being limited by the considerable effort generall...
In the nervous system , every neuronal process should know where to grow and when to establish contacts to the next-order neurons . During development , it is known that neural circuit formation is primarily determined by the genes . To identify these genes , we focused on the Drosophila visual circuitry as a model sys...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "function", "developmental", "biology/neurodevelopment", "genetics", "and", "genomics/gene", "discovery", "neuroscience/neurodevelopment" ]
2008
Systematic Identification of Genes that Regulate Neuronal Wiring in the Drosophila Visual System
Biogenesis of mammalian mitochondrial ribosomes requires a concerted maturation of both the small ( SSU ) and large subunit ( LSU ) . We demonstrate here that the m5C methyltransferase NSUN4 , which forms a complex with MTERF4 , is essential in mitochondrial ribosomal biogenesis as mitochondrial translation is abolishe...
Mitochondria perform a number of essential functions in the cell , including synthesis of ATP via the oxidative phosphorylation ( OXPHOS ) system . Normal mitochondrial function requires coordinated expression of two genomes: mitochondria's own genome ( mtDNA ) , which encodes 13 respiratory chain subunits with essenti...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biochemistry", "rna", "rna", "processing", "nucleic", "acids", "proteins", "protein", "translation", "gene", "expression", "genetics", "biology" ]
2014
NSUN4 Is a Dual Function Mitochondrial Protein Required for Both Methylation of 12S rRNA and Coordination of Mitoribosomal Assembly
Relative to normal tissues , tumor microcirculation exhibits high structural and functional heterogeneity leading to hypoxic regions and impairing treatment efficacy . Here , computational simulations of blood vessel structural adaptation are used to explore the hypothesis that abnormal adaptive responses to local hemo...
Blood vessels of tumors have abnormal structures , being irregular and tortuous . Oxygen supply to tumors is heterogeneous , with regions of low oxygen that resist radiation treatment and some types of chemotherapy . Blood vessels undergo continual structural change ( adaptation ) in response to blood flow and metaboli...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "physiology/cardiovascular", "physiology", "and", "circulation", "oncology/oncology", "agents", "computational", "biology/systems", "biology" ]
2009
Structural Adaptation and Heterogeneity of Normal and Tumor Microvascular Networks
Chagas disease is caused by the parasitic infection of Trypanosoma cruzi ( T . cruzi ) . The STOP CHAGAS clinical trial was initiated in 2011 to evaluate posaconazole in treating Chagas disease , with treatment success defined as negative qualitative PCR results of detecting the parasites in blood specimens collected p...
Chagas disease is caused by the infection of the protozoan parasite Trypanosoma cruzi ( T . cruzi ) and carries a significant tropical disease burden in the Western Hemisphere . The STOP CHAGAS study was a global clinical trial evaluating therapies for Chagas disease . PAXgene blood DNA tubes used by the study simplifi...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion", "Conclusions" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "organisms", "protozoans", "dna", "replication", "neglected", "tropical", "diseases", "molecular", "biology", "techniques", "dna", "cel...
2016
Development of a PCR Assay to Detect Low Level Trypanosoma cruzi in Blood Specimens Collected with PAXgene Blood DNA Tubes for Clinical Trials Treating Chagas Disease
HIV reservoirs and production of viral antigens are not eliminated in chronically infected participants treated with combination antiretroviral therapy ( cART ) . Novel therapeutic strategies aiming at viral reservoir elimination are needed to address chronic immune dysfunction and non-AIDS morbidities that exist despi...
Current HIV therapies prevent AIDS by dramatically reducing , but not eliminating , HIV infection . A reservoir of HIV-infected cells persists during long-term antiviral therapy , and individuals are at increased risk to develop non-AIDS illnesses , e . g . , accelerated heart , bone , or kidney disease . Novel strateg...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Targeting HIV Reservoir in Infected CD4 T Cells by Dual-Affinity Re-targeting Molecules (DARTs) that Bind HIV Envelope and Recruit Cytotoxic T Cells
Reduced DNA methylation has been reported in DICER1-deficient mouse ES cells . Reductions seen at pericentric satellite repeats have suggested that siRNAs are required for the proper assembly of heterochromatin . More recent studies have postulated that the reduced methylation is an indirect effect: the loss of Mir290 ...
In mammalian cells , DNA methylation is required for the maintenance of genome stability . Recent studies have shown that the genome-wide levels of DNA methylation can be reduced in DICER1-deficient mouse embryonic stem ( ES ) cells , suggesting that the activity of DNA methylating enzymes ( DNMTs ) may be regulated by...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "developmental", "biology", "genetics", "biology", "molecular", "cell", "biology", "genetics", "and", "genomics" ]
2012
Normal DNA Methylation Dynamics in DICER1-Deficient Mouse Embryonic Stem Cells
Faithful chromosome segregation during meiosis I depends on the establishment of a crossover between homologous chromosomes . This requires induction of DNA double-strand breaks ( DSBs ) , alignment of homologs , homolog association by synapsis , and repair of DSBs via homologous recombination . The success of these ev...
During meiosis , one round of replication is followed by two divisions to produce haploid gametes . During the first meiotic division , faithful segregation is facilitated by the formation between the parental homologs of a physical tether called crossover . Crossovers require the introduction of DNA double-strand brea...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2013
Matefin/SUN-1 Phosphorylation Is Part of a Surveillance Mechanism to Coordinate Chromosome Synapsis and Recombination with Meiotic Progression and Chromosome Movement
Timely , and sometimes rapid , metabolic adaptation to changes in food supply is critical for survival as an organism moves from the fasted to the fed state , and vice versa . These transitions necessitate major metabolic changes to maintain energy homeostasis as the source of blood glucose moves away from ingested car...
Homeostasis of blood glucose concentrations during circadian shifts in survival-related activities , sleep and food availability is crucial for the survival of mammals . This process depends upon glucose intake , short-term storage as glycogen , and gluconeogenesis . The integration of hepatic glycogen anabolic and cat...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "systems", "biology", "mathematics", "applied", "mathematics", "biology", "computational", "biology", "nonlinear", "dynamics" ]
2011
A Whole-Body Model for Glycogen Regulation Reveals a Critical Role for Substrate Cycling in Maintaining Blood Glucose Homeostasis
Sinorhizobium meliloti differentiates into persisting , nitrogen-fixing bacteroids within root nodules of the legume Medicago truncatula . Nodule-specific cysteine-rich antimicrobial peptides ( NCR AMPs ) and the bacterial BacA protein are essential for bacteroid development . However , the bacterial factors central to...
Certain bacterial species have the unique capacity to enter into eukaryotic host cells and establish prolonged infections , which can be beneficial ( e . g . bacterial-legume symbiosis ) or detrimental ( e . g . chronic disease ) for the host . However , the mechanisms by which bacteria persist in host cells are poorly...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "plant", "science", "plant", "microbiology", "plant", "biology", "microbial", "pathogens", "plant", "cell", "biology", "biology", "microbial", "growth", "and", "development", "microbiology", "host-pathogen", "interaction", "bacterial", "pathoge...
2011
Protection of Sinorhizobium against Host Cysteine-Rich Antimicrobial Peptides Is Critical for Symbiosis
Dose response in micro-parasite infections is usually shallower than predicted by the independent action model , which assumes that each infectious unit has a probability of infection that is independent of the presence of other infectious units . Moreover , the prevalence of mixed-genotype infections was greater than ...
What elements are indispensable in the description of the most basic host-pathogen interactions ? The simplest models of infection generally fail to predict how many host plants or animals will become infected , and which virus genotypes will be present in these infected hosts . These simple models of infection are the...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "population", "ecology", "ecology", "population", "modeling", "virology", "biology", "computational", "biology", "microbiology", "host-pathogen", "interaction" ]
2011
Heterogeneous Host Susceptibility Enhances Prevalence of Mixed-Genotype Micro-Parasite Infections
The hierarchical relationships between stem cells and progenitors that guide mammary gland morphogenesis are still poorly defined . While multipotent basal stem cells have been found within the myoepithelial compartment , the in vivo lineage potential of luminal progenitors is unclear . Here we used the expression of t...
Tissue-specific stem cells are believed to be multipotent , thus able to generate all cell types of their tissue of origin . In the mammary gland epithelium , however , the existence of multipotent versus unipotent adult stem cells is currently under debate . In this study , we have identified and characterized a popul...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Luminal Progenitors Restrict Their Lineage Potential during Mammary Gland Development
Despite of a high disease burden , mainly in Latin America , Chagas disease ( CD ) is underdiagnosed and undertreated . Rapid diagnostic tests ( RDTs ) might improve the access to diagnosis . The aim of this study is to review the accuracy of commercially available RDTs used in field conditions for the diagnosis of chr...
Chagas disease ( CD ) is a parasitic disease that can affect seriously the health status of affected individuals . People with CD , mainly living in remote areas of Latin America , often face major barriers to the disease recognition , diagnosis and treatment . The World Health Organization recommends the combined use ...
[ "Abstract", "Introduction", "Methods", "Inclusion", "criteria", "Results", "Discussion" ]
[ "recombination-based", "assay", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "biochemical", "analysis", "enzyme", "assays",...
2019
Rapid immunochromatographic tests for the diagnosis of chronic Chagas disease in at-risk populations: A systematic review and meta-analysis
Mitochondrial antiviral-signaling protein ( MAVS ) transmits signals from RIG-I-like receptors after RNA virus infections . However , the mechanism by which MAVS activates downstream components , such as TBK1 and IKKα/β , is unclear , although previous work suggests the involvement of NEMO or TBK1-binding proteins TANK...
Innate immunity is the first line of defense against virus infection . RIG-I-like receptors ( RLRs ) recognize various viral RNA from RNA viruses and initiate host antiviral responses to produce type I interferons ( IFNs ) and other cytokines . RLRs sense distinct types of viruses by sharing a common adaptor protein ca...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "reverse", "transcriptase-polymerase", "chain", "reaction", "phosphorylation", "precipitates", "enzyme-linked", "immunoassays", "hela", "cells", "293t", "cells", "enzymes", "biological", "cultures", "enzymology", "immunoprecipitation", "cell", "cultures", "molecular", "biolog...
2017
MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner
Molecular recognition is determined by the structure and dynamics of both a protein and its ligand , but it is difficult to directly assess the role of each of these players . In this study , we use Markov State Models ( MSMs ) built from atomistic simulations to elucidate the mechanism by which the Lysine- , Arginine-...
Protein-ligand interactions are crucial to chemistry , biology and medicine . Many studies have been conducted to probe the mechanism of protein-ligand binding , leading to the development of the induced fit and conformational selection models . Unfortunately , experimentally probing the atomistic details of protein-li...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biomacromolecule-ligand", "interactions", "physics", "biochemistry", "protein", "chemistry", "protein", "interactions", "proteins", "protein", "structure", "biology", "biophysics", "drug", "discovery" ]
2011
A Role for Both Conformational Selection and Induced Fit in Ligand Binding by the LAO Protein
Kinetically stable proteins , those whose stability is derived from their slow unfolding kinetics and not thermodynamics , are examples of evolution's best attempts at suppressing unfolding . Especially in highly proteolytic environments , both partially and fully unfolded proteins face potential inactivation through d...
Proteins , synthesized as linear polymers of amino acids , fold up into compact native states , burying their hydrophobic amino acids into their interiors . Protein folding minimizes the non-specific interactions that unfolded protein chains can make , which include aggregation with other proteins and degradation by pr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biophysics/protein", "folding", "biochemistry/protein", "folding", "biophysics/theory", "and", "simulation", "biochemistry/theory", "and", "simulation", "computational", "biology/molecular", "dynamics" ]
2010
Unfolding Simulations Reveal the Mechanism of Extreme Unfolding Cooperativity in the Kinetically Stable α-Lytic Protease