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Vibrio parahaemolyticus is an important pathogen that causes food-borne gastroenteritis in humans . The type III secretion system encoded on chromosome 2 ( T3SS2 ) plays a critical role in the enterotoxic activity of V . parahaemolyticus . Previous studies have demonstrated that T3SS2 induces actin stress fibers in var...
Many bacterial pathogens manipulate the actin cytoskeleton of mammalian cells to establish pathogenesis via invasion , to evade killing by phagocytes , to disrupt a barrier function , and to induce inflammation caused by translocation type III secretion ( T3S ) effector proteins . We demonstrated that the T3S effector ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Interaction between the Type III Effector VopO and GEF-H1 Activates the RhoA-ROCK Pathway
Neuronal membrane potential resonance ( MPR ) is associated with subthreshold and network oscillations . A number of voltage-gated ionic currents can contribute to the generation or amplification of MPR , but how the interaction of these currents with linear currents contributes to MPR is not well understood . We explo...
Many neuron types exhibit membrane potential resonance ( MPR ) in which the neuron produces the largest response to oscillatory input at some preferred ( resonant ) frequency and , in many systems , the network frequency is correlated with neuronal MPR . MPR is captured by a peak in the impedance vs . frequency curve (...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "resonance", "frequency", "medicine", "and", "health", "sciences", "neural", "networks", "membrane", "potential", "electrophysiology", "neuroscience", "animals", "crabs", "crustaceans", "ionic", "current", "bioenergetics", "computer", "and", "information", ...
2017
Mechanisms of generation of membrane potential resonance in a neuron with multiple resonant ionic currents
Most utility theories of choice assume that the introduction of an irrelevant option ( called the decoy ) to a choice set does not change the preference between existing options . On the contrary , a wealth of behavioral data demonstrates the dependence of preference on the decoy and on the context in which the options...
While faced with a decision between two options for which you have no clear preference ( say , a small cheap TV and a large expensive TV ) , you are presented with a new but inferior option ( say , a medium expensive TV ) . The mere presence of the new option , which you would not select anyway , shifts your preference...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "cognitive", "neuroscience", "behavioral", "neuroscience", "decision", "making", "computational", "neuroscience", "biology", "computational", "biology", "neuroscience" ]
2012
A Range-Normalization Model of Context-Dependent Choice: A New Model and Evidence
Seminal fluid proteins affect fertility at multiple stages in reproduction . In many species , a male's ejaculate coagulates to form a copulatory plug . Although taxonomically widespread , the molecular details of plug formation remain poorly understood , limiting our ability to manipulate the structure and understand ...
Male reproductive fitness is strongly affected by seminal fluid . In many animals , the male's ejaculate coagulates in the female's reproductive tract to form a structure known as the copulatory plug . Here , I show that male mice without a functional copy of the gene transglutaminase IV cannot form a plug and suffer s...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "genetics", "reproductive", "system", "genetic", "mutation", "sexual", "reproduction", "anatomy", "and", "physiology", "gene", "function", "animal", "models", "model", "organisms", "sexual", "selection", "biology", "mouse", "mutagenesis", "physiology", "geneti...
2013
Genetic Disruption of the Copulatory Plug in Mice Leads to Severely Reduced Fertility
Only limited information is currently available on the prevalence of vector borne and zoonotic pathogens in dogs and ticks in Nigeria . The aim of this study was to use molecular techniques to detect and characterize vector borne pathogens in dogs and ticks from Nigeria . Blood samples and ticks ( Rhipicephalus sanguin...
In Nigeria , dogs are not only kept as pets , but are also used for hunting as well as a source of animal protein among some ethnic groups . Ticks are known to infest dogs and serve as vectors for some pathogens of zoonotic and veterinary importance . There is limited information on the prevalence and distribution of v...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "veterinary", "science" ]
2013
Molecular Detection and Characterization of Tick-borne Pathogens in Dogs and Ticks from Nigeria
Esophageal cancer occurs as either squamous cell carcinoma ( ESCC ) or adenocarcinoma . ESCCs comprise almost 90% of cases worldwide , and recur with a less than 15% five-year survival rate despite available treatments . The identification of new ESCC drivers and therapeutic targets is critical for improving outcomes ....
The DEK oncogene is overexpressed in nearly all human cancers and portends a poor prognosis for many cancer types . High DEK expression causes cancer related phenotypes such as increased cellular proliferation , migration , and invasion in vitro . Despite the well documented link between high DEK expression and cancer ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "keratinocytes", "cancer", "treatment", "carcinomas", "cancers", "and", "neoplasms", "epithelial", "cells", "animal", "models", "oncology", "model", "organisms", "tongue", "experimental", "organism", "systems", "molecular", "biol...
2018
Dek overexpression in murine epithelia increases overt esophageal squamous cell carcinoma incidence
MicroRNAs ( miRNAs ) are 19 to 23 nucleotide–long RNAs that post-transcriptionally regulate gene expression . Human cells express several hundred miRNAs which regulate important biological pathways such as development , proliferation , and apoptosis . Recently , 12 miRNA genes have been identified within the genome of ...
Kaposi sarcoma–associated herpesvirus ( KSHV ) is a gamma-herpesvirus associated with Kaposi sarcoma , primary effusion lymphoma , and a subset of muticentric Castleman disease . Recently , it was found that KSHV encodes 12 microRNAs ( miRNAs ) within its latency-associated region . miRNAs are small ∼22 nucleotide-long...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "microrna", "targets", "kshv", "virology" ]
2007
Identification of Cellular Genes Targeted by KSHV-Encoded MicroRNAs
While sexual reproduction is pervasive in eukaryotic cells , the strategies employed by fungal species to achieve and complete sexual cycles is highly diverse and complex . Many fungi , including Saccharomyces cerevisiae and Schizosaccharomyces pombe , are homothallic ( able to mate with their own mitotic descendants )...
Candida albicans is notorious as a human fungal pathogen that causes millions of incidents of thrush and systemic infection every year . Sexual reproduction plays a pivotal role in the biology and survival of pathogenic fungal pathogens . However , C . albicans is predominantly clonal , suggesting that mating and recom...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "cellular", "stress", "responses", "pathology", "and", "laboratory", "medicine", "chemical", "compounds", "gene", "regulation", "pathogens", "regulatory", "proteins", "cell", "processes", "microbiology", "dna-binding", "proteins", ...
2019
Environment-induced same-sex mating in the yeast Candida albicans through the Hsf1–Hsp90 pathway
The ability of phagocytes to clear pathogens is an essential attribute of the innate immune response . The role of signaling lipid molecules such as phosphoinositides is well established , but the role of membrane sphingolipids in phagocytosis is largely unknown . Using a genetic approach and small molecule inhibitors ...
The fungus Candida albicans is not only a commensal of the digestive system , but also a common cause of human opportunistic infections . Macrophages and dendritic cells can eliminate C . albicans by phagocytosis , a complex process that involves extensive membrane reorganization at the cell surface . The extent to whi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Disruption of Sphingolipid Biosynthesis Blocks Phagocytosis of Candida albicans
HIV-1 can downregulate HLA-C on infected cells , using the viral protein Vpu , and the magnitude of this downregulation varies widely between primary HIV-1 variants . The selection pressures that result in viral downregulation of HLA-C in some individuals , but preservation of surface HLA-C in others are not clear . To...
HLA-C is a member of the major histocompatibility complex class-I ( MHC-I ) family of molecules which are integral to many responses of innate and adaptive immunity . HIV-1 can downregulate the expression level of HLA-C on infected cells , using the viral protein Vpu , but the magnitude of HLA-C downregulation varies w...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "transfection", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "pathogens", "variant", "genotypes", "immunology", "microbiology", "cloning", "genetic", "mapping", "retroviruses", "viruses", "im...
2018
HLA-C downregulation by HIV-1 adapts to host HLA genotype
Defining the most penetrating correlates of protective memory T cells is key for designing improved vaccines and T cell therapies . Here , we evaluate how interleukin ( IL-2 ) production by memory CD4 T cells , a widely held indicator of their protective potential , impacts immune responses against murine influenza A v...
We show that memory CD4 T cell mediated protection against influenza A virus is independent of the signature multifunctional cytokine IL-2 that is thought to define the most protective memory cells . IL-2 deficient cells are more effective than wild-type memory cells on a per cell basis at combating IAV and drive tempe...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "t", "helper", "cells", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "respiratory", "infections", "cytokines", "pathogens", "immunology...
2019
Memory CD4 T cell-derived IL-2 synergizes with viral infection to exacerbate lung inflammation
Structural diversity in the peptide binding sites of the redundant classical MHC antigen presenting molecules is strongly selected in humans and mice . Although the encoded antigen presenting molecules overlap in antigen presenting function , differences in polymorphism at the MHC I A , B and C loci in humans and highe...
MHC I genes are best understood as regulators of antiviral immunity . In humans and mice there are 2 to 3 homologous MHC I genes encoding highly polymorphic antigen presenting molecules which present virus proteins to T lymphocytes . A world wide effort has catalogued more than 6 , 300 classical HLA MHC I alleles in hu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "model", "organisms", "genetics", "population", "genetics", "biology", "immunology", "population", "biology", "neuroscience", "genetics", "and", "genomics" ]
2012
Nonequivalence of Classical MHC Class I Loci in Ability to Direct Effective Antiviral Immunity
DNA methylation acts in concert with restriction enzymes to protect the integrity of prokaryotic genomes . Studies in a limited number of organisms suggest that methylation also contributes to prokaryotic genome regulation , but the prevalence and properties of such non-restriction-associated methylation systems remain...
DNA methylation is a chemical modification of DNA present in many prokaryotic genomes . The best-known role of DNA methylation is as a component of restriction-modification systems . In these systems , restriction enzymes target foreign DNA for cleavage , while DNA methylation protects the host genome from destruction ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "sequencing", "techniques", "gene", "regulation", "regulator", "genes", "dna", "replication", "genome", "analysis", "gene", "types", "sequence", "motif", "analysis", "molecular", "biology", "techniques", "epigenetics", "dna", "dna", "methylation", "chromatin", "research...
2016
The Epigenomic Landscape of Prokaryotes
Non-enveloped viruses penetrate host membranes to infect cells . A cell-based assay was used to probe the endoplasmic reticulum ( ER ) -to-cytosol membrane transport of the non-enveloped SV40 . We found that , upon ER arrival , SV40 is released into the lumen and undergoes sequential disulfide bond disruptions to reach...
Biological membranes represent a major barrier during viral infection . While the mechanism by which an enveloped virus breaches the limiting membrane of a host cell is well-characterized , this membrane penetration process is poorly understood for non-enveloped viruses . Indeed , most available insights on membrane tr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology", "biology", "microbiology" ]
2011
A Large and Intact Viral Particle Penetrates the Endoplasmic Reticulum Membrane to Reach the Cytosol
Sex determination is a hierarchically-regulated process with high diversity in different organisms including insects . The W chromosome-derived Fem piRNA has been identified as the primary sex determination factor in the lepidopteran insect , Bombyx mori , revealing a distinctive piRNA-mediated sex determination pathwa...
Sex determination is an essential and universal process for metazoan reproduction and development . Insect sex determination is highly diverse , especially for the primary signal and transductory genes . Mechanism of sex determination in the model lepidopteran insect , Bombyx mori , is largely unknown , although a piRN...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "reproductive", "system", "dna-binding", "proteins", "animals", "reproductive", "physiology", "animal", "models", "developmental", "biology", "drosophila", "melanogaster", "model", "organisms", "silkworms", "immunopr...
2018
Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination
Genome-wide association analysis in populations of European descent has recently found more than a hundred genetic variants affecting risk for common disease . An open question , however , is how relevant the variants discovered in Europeans are to other populations . To address this problem for cardiovascular phenotyp...
Single-base changes in DNA can affect biochemical measures , such as blood cholesterol or lipid levels . Such changes or “variants” can be associated with a trait either because they cause the trait or because they are linked to other causal variants . In either case , the associated variant ( s ) may be useful in pred...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cardiovascular", "disorders/coronary", "artery", "disease", "genetics", "and", "genomics" ]
2009
Genetic Differences between the Determinants of Lipid Profile Phenotypes in African and European Americans: The Jackson Heart Study
Nucleoporins are the constituents of nuclear pore complexes ( NPCs ) and are essential regulators of nucleocytoplasmic transport , gene expression and genome stability . The nucleoporin MEL-28/ELYS plays a critical role in post-mitotic NPC reassembly through recruitment of the NUP107-160 subcomplex , and is required fo...
Most animal cells have a nucleus that contains the genetic material: the chromosomes . The nucleus is enclosed by the nuclear envelope , which provides a physical barrier between the chromosomes and the surrounding cytoplasm , and enables precisely controlled transport of proteins into and out of the nucleus . Transpor...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "caenorhabditis", "metaphase", "cell", "cycle", "and", "cell", "division", "cell", "processes", "light", "microscopy", "animals", "animal", "models", "mitosis", "germ", "cells", "developmental", "biology", "caenorhabditis", "elegans", "oocytes", "model"...
2016
Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation
Based on our initial observations showing that mice consuming a probiotic product develop more severe cryptosporidiosis , we investigated the impact of other dietary interventions on the intracellular proliferation of Cryptosporidium parvum and C . tyzzeri in the mouse . Mice were orally infected with oocysts and paras...
The infection with Cryptosporidium parasite , a condition known as cryptosporidiosis , is a common cause of infant diarrhea in developing countries . We have previously shown that mice infected with C . parvum , one of the main cause of human cryptosporidiosis , develop a more severe infection if given probiotics . To ...
[ "Abstract", "Introduction", "Material", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "parasite", "groups", "oocysts", "microbiome", "microbiology", "diet", "cryptosporidium", "parasitic", "diseases", "parasitic", "protozoans", "parasitology", "apicomplexa", "nutrition", "protozoans", "cryptosporidiosis", "microbial", ...
2019
Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis
Immunity to the sand fly salivary protein SALO ( Salivary Anticomplement of Lutzomyia longipalpis ) protected hamsters against Leishmania infantum and L . braziliensis infection and , more recently , a vaccine combination of a genetically modified Leishmania with SALO conferred strong protection against L . donovani in...
Immunity to sand fly salivary proteins has been shown to confer protection against leishmaniasis in rodent models . Recombinant salivary protein SALO ( Salivary Anticomplement of Lutzomyia longipalpis ) was shown to protect hamsters against the fatal outcome of visceral leishmaniasis caused by Leishmania infantum and t...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "immune", "physiology", "pichia", "pastoris", "immunology", "tropical", "diseases", "sand", "flies", "parasitic", "diseases", "vaccines", "fungi", "protein", "structure", "neglected", "tropical", "diseases", "infectious", "diseas...
2017
Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis
The emergence and spread of antibiotic resistance among Acinetobacter spp . have been investigated extensively . Most studies focused on the multiple antibiotic resistance genes located on plasmids or genomic resistance islands . On the other hand , the mechanisms controlling intrinsic resistance are still not well und...
The level of interest in intrinsic resistance genes has increased recently , and one of reasons is that their mobilization could lead to emergence of resistant pathogens . Insertion sequences ( ISs ) or plasmids can capture intrinsic resistance genes and disseminate them in bacterial populations . In this study , we id...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "antimicrobials", "taxonomy", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "drugs", "microbiology", "antibiotic", "resistance", "streptomycin", "phylogenetics", "data", "management", "antibiotics", "phylogenetic", "anal...
2017
A new subclass of intrinsic aminoglycoside nucleotidyltransferases, ANT(3")-II, is horizontally transferred among Acinetobacter spp. by homologous recombination
Intestinal schistosomiasis and soil-transmitted helminth ( STH ) infections constitute major public health problems in many parts of sub-Saharan Africa . In this study we examined the functional significance of such polyparasite infections in anemia and undernutrition in Rwandan individuals . Three polyparasite infecti...
The helminth infections—schistosomiasis , hookworm , ascariasis and trichuriasis—are the main neglected tropical diseases ( NTDs ) to thrive in sub-Saharan Africa . Here we assess the distribution and the intensities of such polyparasite infections in two districts of the Northern Province in Rwanda and determine wheth...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "public", "health", "and", "epidemiology/epidemiology", "infectious", "diseases/helminth", "infections", "public", "health", "and", "epidemiology/global", "health", "public", "health", "and", "epidemiology/infectious", "diseases", "mathematics/statistics" ]
2009
Polyparasite Helminth Infections and Their Association to Anaemia and Undernutrition in Northern Rwanda
The last 20 years has seen a significant series of outbreaks of Buruli/Bairnsdale Ulcer ( BU ) , caused by Mycobacterium ulcerans , in temperate south-eastern Australia ( state of Victoria ) . Here , the prevailing view of M . ulcerans as an aquatic pathogen has been questioned by recent research identifying native wil...
Mycobacterium ulcerans causes the disfiguring human skin disease Buruli ulcer ( BU ) . The mechanism of transmission and reservoir for human infection remain unknown . In previous research , we reported the detection of M . ulcerans DNA in the faeces of possums ( small tree-dwelling marsupials ) in an area of South-Eas...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "bacteriology", "emerging", "infectious", "diseases", "medical", "microbiology", "biology", "microbiology", "bacterial", "pathogens" ]
2014
Potential Wildlife Sentinels for Monitoring the Endemic Spread of Human Buruli Ulcer in South-East Australia
Poxviruses have evolved unique proteins and mechanisms to counteract the nuclear factor κB ( NF-κB ) signaling pathway , which is an essential regulatory pathway of host innate immune responses . Here , we describe a NF-κB inhibitory virion protein of orf virus ( ORFV ) , ORFV073 , which functions very early in infecte...
Successful infection of the host by poxviruses relies on control of innate immune responses by virus-encoded immunomodulators . In particular , poxviruses evolved to counteract the NF-κB pathway by encoding multiple inhibitors targeting various levels of NF-κB signaling . We identified a NF-κB inhibitor encoded by ORFV...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "phosphorylation", "medicine", "and", "health", "sciences", "immune", "physiology", "chemical", "compounds", "ruminants", "immunology", "microbiology", "vertebrates", "viral", "structure", "animals", "mammals", "esters", "dna", "transcription", "signal", "inhibition", "am...
2017
A parapoxviral virion protein inhibits NF-κB signaling early in infection
The anthelmintic praziquantel ( ±PZQ ) serves as a highly effective antischistosomal therapy . ±PZQ causes a rapid paralysis of adult schistosome worms and deleterious effects on the worm tegument . In addition to these activities against the parasite , ±PZQ also modulates host vascular tone in blood vessels where the ...
Praziquantel is a key drug for combating diseases caused by parasitic flatworms . It is the therapeutic mainstay for treatment of schistosomiasis , a disease that afflicts over 200 million people worldwide . In this study , we investigate potential molecular targets of praziquantel , and demonstrate interactions with s...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Host", "target(s)", "of", "±PZQ", "Parasite", "target(s)", "of", "±PZQ" ]
[ "schistosoma", "fluorescence", "imaging", "invertebrates", "medicine", "and", "health", "sciences", "stereoisomers", "chemical", "compounds", "helminths", "cardiovascular", "anatomy", "membrane", "potential", "mesenteric", "arteries", "electrophysiology", "neuroscience", "ani...
2018
Activation of host transient receptor potential (TRP) channels by praziquantel stereoisomers
Cercospora zeae-maydis causes gray leaf spot of maize , which has become one of the most widespread and destructive diseases of maize in the world . C . zeae-maydis infects leaves through stomata , which is predicated on the ability of the pathogen to perceive stomata and reorient growth accordingly . In this study , t...
Fungal diseases of crop plants are a significant threat to global food security . Improving host resistance is the most cost-effective and environmentally sound strategy for sustainable disease management . However , many devastating diseases of important crops have proven impossible to manage through genetic resistanc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "microbial", "metabolism", "sustainable", "agriculture", "plant", "biology", "microbiology", "host-pathogen", "interaction", "developmental", "biology", "plant", "science", "microbial", "growth", "and", "development", "fungal", "reproduction", "morphogenesis", "mycology", "...
2011
Regulation of Stomatal Tropism and Infection by Light in Cercospora zeae-maydis: Evidence for Coordinated Host/Pathogen Responses to Photoperiod?
Antiretroviral therapy ( ART ) effectively controls HIV infection , suppressing HIV viral loads . However , some residual virus remains , below the level of detection , in HIV-infected patients on ART . The source of this viremia is an area of debate: does it derive primarily from activation of infected cells in the la...
In HIV+ individuals , antiretroviral therapy ( ART ) effectively controls HIV viral loads to below levels detectable by routine tests . However , more sensitive tests can detect some residual viremia . The source of this virus is a matter of debate: does it derive from ongoing viral replication , or from viral producti...
[ "Abstract", "Introduction", "Models", "Discussion" ]
[]
2016
Residual Viremia in Treated HIV+ Individuals
As an inhibitor of cyclin-dependent kinases , p16INK4A is an important tumour suppressor and inducer of cellular senescence that is often inactivated during the development of cancer by promoter DNA methylation . Using newly established lymphoblastoid cell lines ( LCLs ) expressing a conditional EBNA3C from recombinant...
We previously showed that two Epstein-Barr virus latency-associated proteins—EBNA3A and EBNA3C—contribute to enhanced B cell survival by inhibiting the expression of the death-inducing protein BIM . This repression involves remodelling of the BIM gene promoter by polycomb proteins and DNA methylation within an unusuall...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "molecular", "biology/histone", "modification", "virology/persistence", "and", "latency", "molecular", "biology/transcription", "initiation", "and", "activation", "cell", "biology/cellular", "death", "and", "stress", "responses", "cell", "biology/cell", "growth", "and", "di...
2010
Epigenetic Repression of p16INK4A by Latent Epstein-Barr Virus Requires the Interaction of EBNA3A and EBNA3C with CtBP
There is an increasing need to evaluate the impact of chemotherapeutic and vector-based interventions as onchocerciasis affected countries work towards eliminating the disease . The Esperanza Window Trap ( EWT ) provides a possible alternative to human landing collections ( HLCs ) for the collection of anthropophilic b...
Using human bait to collect blood-feeding insects is an ethically sensitive issue . Whereas researchers investigating insect-borne diseases such as sleeping sickness , leishmaniasis and malaria have a range of traps at their disposal , those investigating blackflies and river blindness ( onchocerciasis ) still rely on ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "onchocerca", "volvulus", "chemical", "compounds", "ruminants", "helminths", "geographical", "locations", "tropical", "diseases", "uganda", "vertebrates", "parasitic", "diseases", "animals", "mammals", "diptera", "...
2017
Esperanza Window Traps for the collection of anthropophilic blackflies (Diptera: Simuliidae) in Uganda and Tanzania
Pathogens hijack host endocytic pathways to force their own entry into eukaryotic target cells . Many bacteria either exploit receptor-mediated zippering or inject virulence proteins directly to trigger membrane reorganisation and cytoskeletal rearrangements . By contrast , extracellular C . trachomatis elementary bodi...
Chlamydia trachomatis remains the leading bacterial agent of sexually transmitted disease worldwide and causes a form of blindness called trachoma in Developing nations , which is recognised by the World Health Organisation as a neglected tropical disease . Despite this burden , we know comparatively little about how i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "intracellular", "pathogens", "gene", "regulation", "chlamydia", "trachomatis", "pathogens", "immunology", "microbiology", "sexually", "transmitted", "diseases", ...
2018
Chlamydia exploits filopodial capture and a macropinocytosis-like pathway for host cell entry
Histone demethylases have emerged as important players in developmental processes . Jumonji domain containing-3 ( Jmjd3 ) has been identified as a key histone demethylase that plays a critical role in the regulation of gene expression; however , the in vivo function of Jmjd3 in embryonic development remains largely unk...
A chromosome in the eukaryotic nucleus is an organized package of DNA coiled around histone proteins . DNA contains genes and other nucleotide sequences as well as histone proteins including H1 , H2A , H2B , H3 , and H4 . Gene expression is dynamically regulated by DNA and histone modifications , such as methylation an...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "histology", "anatomy", "genetics", "biology", "and", "life", "sciences" ]
2014
Stage-Dependent and Locus-Specific Role of Histone Demethylase Jumonji D3 (JMJD3) in the Embryonic Stages of Lung Development
Chagas' disease is an important neglected public health problem in many Latin American countries , but population-based epidemiological data are scarce . Here we present a nationwide analysis on Chagas-associated mortality , and risk factors for death from this disease . We analyzed all death certificates of individual...
American trypanosomiasis ( Chagas' disease ) is a parasitic disease which remains a public health problem in Latin America , but studies investigating the dynamics in populations under risk are scarce . We conducted a nation-wide study based on about 9 million Brazilian death certificates from 1999–2007 . Epidemiologic...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "public", "health", "and", "epidemiology", "epidemiology", "global", "health", "public", "health" ]
2012
Epidemiology of Mortality Related to Chagas' Disease in Brazil, 1999–2007
Recently , a growing number of biological research and scientific experiments have demonstrated that microRNA ( miRNA ) affects the development of human complex diseases . Discovering miRNA-disease associations plays an increasingly vital role in devising diagnostic and therapeutic tools for diseases . However , since ...
Identifying potential miRNA-disease associations enhances the understanding towards molecular mechanisms and pathogenesis of diseases , which is beneficial for the development of diagnostic/treatment tools for diseases . Compared with traditional experiment methods , computational models can help experimenters reduce t...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "linguistics", "gene", "regulation", "applied", "mathematics", "cancers", "and", "neoplasms", "social", "sciences", "simulation", "and", "modeling", "oncology", "algorithms", "hematologic", "cancers", "and", "related", "disorders...
2018
MDHGI: Matrix Decomposition and Heterogeneous Graph Inference for miRNA-disease association prediction
An outstanding problem in neuroscience is to understand how information is integrated across the many modules of the brain . While classic information-theoretic measures have transformed our understanding of feedforward information processing in the brain’s sensory periphery , comparable measures for information flow i...
Information theory has been key to our understanding of the feedforward pathways of the brain’s sensory periphery . But , traditional information-theoretic measures only quantify communication between pairs of transmitters and receivers , and have been of limited utility in decoding signals in the recurrent networks th...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "cluster", "analysis", "medicine", "and", "health", "sciences", "neural", "networks", "nervous", "system", "applied", "mathematics", "vertebrates", "neuroscience", "animals", "mammals", "simulation", "and", "modeling", "algorithms", "primates", "mathematics", "network", ...
2019
Information integration in large brain networks
Plasmacytoid dendritic cell ( pDC ) -mediated protection against cytopathic virus infection involves various molecular , cellular , tissue-scale , and organism-scale events . In order to better understand such multiscale interactions , we have implemented a systems immunology approach focusing on the analysis of the st...
Human infections with highly virulent viruses , such as 1918 influenza or SARS-coronavirus , represent major threats to public health . The initial innate immune responses to such viruses have to restrict virus spread before the adaptive immune responses fully develop . Therefore , it is of fundamental practical import...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/animal", "models", "of", "infection", "immunology/innate", "immunity", "computer", "science/numerical", "analysis", "and", "theoretical", "computing", "computational", "biology/systems", "biology", "virology/host", "antiviral", "responses" ]
2010
A Systems Immunology Approach to Plasmacytoid Dendritic Cell Function in Cytopathic Virus Infections
It has long been suspected that the rate of mutation varies across the human genome at a large scale based on the divergence between humans and other species . However , it is now possible to directly investigate this question using the large number of de novo mutations ( DNMs ) that have been discovered in humans thro...
Using a dataset of more than 130 , 000 de novo mutations we show that there is large-scale variation in the mutation rate at the 100KB and 1MB scales . We show that different types of mutation vary in concert and in a manner that is not expected to generate variation in base composition; hence mutation bias is not resp...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "split-decomposition", "method", "vertebrates", "human", "genomics", "animals", "mammals", "primates", "multiple", "alignment", "calculation", "mutation", "substitution", "mutation", "genome", "analysis", "dna", "recombination", "mammalian", "genomics", "dna", "gene", "co...
2018
Large scale variation in the rate of germ-line de novo mutation, base composition, divergence and diversity in humans
Pneumococcal conjugate vaccines ( PCVs ) have substantially reduced morbidity and mortality of pneumococcal disease . The impact of the 7-valent PCV on all-serotype invasive pneumococcal disease ( IPD ) among children was reported to vary between high-income countries . We investigate the ability to predict this hetero...
Pneumococcal vaccines ( PCVs ) that protect children against 7 , 10 and 13 of the most pathogenic pneumococcal serotypes have substantially reduced childhood morbidity and mortality . A recent analysis that evaluated the impact of the 7 valent PCV in multiple high income settings in North America , Europe and Oceania f...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
The Serotype Distribution among Healthy Carriers before Vaccination Is Essential for Predicting the Impact of Pneumococcal Conjugate Vaccine on Invasive Disease
A wide range of biological processes are regulated by sumoylation , a post-translational modification involving the conjugation of SUMO ( Small Ubiquitin-Like Modifier ) to protein . In Arabidopsis thaliana , AtSIZ1 encodes a SUMO E3 ligase for SUMO modification . siz1 mutants displayed defective secondary cell walls (...
Secondary cell wall ( SCW ) is essential for upright plant growth and long-distance transport of water and solutes . Regulation of SCW formation at the transcriptional level has been much studied . Here we show that AtSIZ1 , a small ubiquitin-related modifier ( SUMO ) E3 ligase , mediates the sumoylation of transcripti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "biotechnology", "plant", "anatomy", "engineering", "and", "technology", "gene", "regulation", "brassica", "plant", "science", "model", "organisms", "sumoylation", "genetically", "modified", "plants", "experimental", "organism", "systems", "inflorescences", "plants", "flo...
2019
SUMO modification of LBD30 by SIZ1 regulates secondary cell wall formation in Arabidopsis thaliana
Most available drugs against visceral leishmaniasis are toxic , and growing limitations in available chemotherapeutic strategies due to emerging resistant strains and lack of an effective vaccine against visceral leishmaniasis deepens the crisis . Antineoplastic drugs like miltefosine have in the past been effective ag...
Leishmaniasis , a neglected tropical disease ( NTD ) caused by Leishmania , has been put on the World Health Organization agenda for eradication as a part of their Special Programme for Tropical Diseases Research . Visceral leishmaniasis ( VL ) is a life-threatening disease when no treatment is given . Most of the drug...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine" ]
2012
Evaluation of Nephroprotective and Immunomodulatory Activities of Antioxidants in Combination with Cisplatin against Murine Visceral Leishmaniasis
Transcription factors are key components of regulatory networks that control development , as well as the response to environmental stimuli . We have established an experimental pipeline in Caenorhabditis elegans that permits global identification of the binding sites for transcription factors using chromatin immunopre...
The C . elegans transcription factor PHA-4 is a member of the highly conserved FOXA family of transcription factors . These factors act as master regulators of organ development by controlling how genes are turned off and on as tissues are formed . Additionally they regulate genes in response to nutrient levels and con...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genomics", "genetics", "and", "genomics/functional", "genomics", "genetics", "and", "genomics/genome", "projects", "genetics", "and", "genomics/gene", "function", "developmental", "biology/organogenesis", "genetics", "and", "genomics" ]
2010
Genome-Wide Identification of Binding Sites Defines Distinct Functions for Caenorhabditis elegans PHA-4/FOXA in Development and Environmental Response
While the hypothalamo-pituitary-adrenal axis ( HPA ) activates a general stress response by increasing glucocorticoid ( Gc ) synthesis , biological stress resulting from infections triggers the inflammatory response through production of cytokines . The pituitary gland integrates some of these signals by responding to ...
Global biological responses involve pleiotropic , general components exhibited by many cells/tissues together with cell-specific responses . Typically , such responses are dependent on multiple signaling pathways that integrate different inputs to trigger concerted tissue/cell responses . In studying LIF action in the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genomics", "cell", "biology/gene", "expression", "genetics", "and", "genomics/functional", "genomics", "genetics", "and", "genomics/gene", "expression", "computational", "biology/molecular", "genetics", "immunology/immune", "response", "immunology/i...
2008
Regulatory Network Analyses Reveal Genome-Wide Potentiation of LIF Signaling by Glucocorticoids and Define an Innate Cell Defense Response
X-chromosome inactivation ( XCI ) in female lymphocytes is uniquely regulated , as the inactive X ( Xi ) chromosome lacks localized Xist RNA and heterochromatin modifications . Epigenetic profiling reveals that Xist RNA is lost from the Xi at the pro-B cell stage and that additional heterochromatic modifications are gr...
Females are predisposed to develop various autoimmune disorders , and the genetic basis for this susceptibility is the X-chromosome . X-linked genes are dosage compensated between sexes by X-chromosome Inactivation ( XCI ) during embryogenesis and maintained into adulthood . Here we show that the chromatin of the inact...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "rna-binding", "proteins", "medicine", "and", "health", "sciences", "immune", "cells", "immunology", "bone", "marrow", "cells", "x-linked", "traits", "rna", "isolation", "molecular", "biology", "techniques", "epigenetics", "chromatin", "research", "a...
2017
Loss of Xist RNA from the inactive X during B cell development is restored in a dynamic YY1-dependent two-step process in activated B cells
Meconium ileus ( MI ) , a life-threatening intestinal obstruction due to meconium with abnormal protein content , occurs in approximately 15 percent of neonates with cystic fibrosis ( CF ) . Analysis of twins with CF demonstrates that MI is a highly heritable trait , indicating that genetic modifiers are largely respon...
Cystic fibrosis ( CF ) is a monogenic disease with considerable phenotypic variability . About 15% of newborns with CF suffer from an intestinal obstruction called meconium ileus ( MI ) , and studies in CF twins have shown that modifier genes play a substantial role in the development of this complication . We used a f...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "biology", "human", "genetics", "genetics", "and", "genomics" ]
2012
Variation in MSRA Modifies Risk of Neonatal Intestinal Obstruction in Cystic Fibrosis
The functional effects of most amino acid replacements accumulated during molecular evolution are unknown , because most are not observed naturally and the possible combinations are too numerous . We created 168 single mutations in wild-type Escherichia coli isopropymalate dehydrogenase ( IMDH ) that match the differen...
Many bioinformatics and functional genomics predictions are derived from evolutionary patterns of amino acid replacement in protein sequence alignments . Most computational methods assume that replacements in one sequence will be tolerated in all related sequences . Here , we evaluate—by direct experiment—the functiona...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "biochemistry/molecular", "evolution", "evolutionary", "biology/microbial", "evolution", "and", "genomics" ]
2010
Pervasive Cryptic Epistasis in Molecular Evolution
Cestodes are a diverse group of parasites , some of them being agents of neglected diseases . In cestodes , little is known about the functional properties of G protein coupled receptors ( GPCRs ) which have proved to be highly druggable targets in other organisms . Notably , serotoninergic G-protein coupled receptors ...
Cestode parasites are flatworms with the ability to parasitize almost every vertebrate species . Several of these parasites are etiological agents of neglected diseases prioritized by WHO , such as hydatid disease , or hydatidosis , a zoonosis caused by species of the genus Echinococcus that affects millions of people ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "neurochemistry", "cestodes", "helminths", "split-decomposition", "method", "neuroscience", "animals", "parasitic", "diseases", "serotonin", "multiple", "alignment", "calculation", "sequence", "motif", "analysis", "n...
2018
Unique pharmacological properties of serotoninergic G-protein coupled receptors from cestodes
A key question in decision-making is how people integrate amounts and probabilities to form preferences between risky alternatives . Here we rely on the general principle of integration-to-boundary to develop several biologically plausible process models of risky-choice , which account for both choices and response-tim...
Decision-making under risk requires a selection between alternatives , such as lotteries , which offer a reward with a specified probability . Human decision between such alternatives is at the center of the normative decision theory , which assumes that decisions are rationally made by forming a value for each alterna...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "infographics", "medicine", "and", "health", "sciences", "decision", "theory", "decision", "making", "statistics", "applied", "mathematics", "social", "sciences", "neuroscience", "research", "design", "cognitive", "psychology", "mathematics", "cognition", "research", "and...
2019
The formation of preference in risky choice
In humans , the absence or irreversible loss of hair cells , the sensory mechanoreceptors in the cochlea , accounts for a large majority of acquired and congenital hearing disorders . In the auditory and vestibular neuroepithelia of the inner ear , hair cells are accompanied by another cell type called supporting cells...
By screening for regeneration deficient zebrafish mutations , we identified a zebrafish mutant line deficient in a highly specific regeneration process , the renewal of hair cells in the lateral line . Although this organ is specific to fish and amphibians , it contains essentially the same mechanosensory cells ( the h...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "function", "genetics", "and", "genomics/gene", "discovery", "developmental", "biology/stem", "cells" ]
2009
Phoenix Is Required for Mechanosensory Hair Cell Regeneration in the Zebrafish Lateral Line
Cotton bacterial blight ( CBB ) , an important disease of ( Gossypium hirsutum ) in the early 20th century , had been controlled by resistant germplasm for over half a century . Recently , CBB re-emerged as an agronomic problem in the United States . Here , we report analysis of cotton variety planting statistics that ...
Cotton bacterial blight ( CBB ) , caused by Xanthomonas citri pv . malvacearum ( Xcm ) , significantly limited cotton yields in the early 20th century but has been controlled by classical resistance genes for more than 50 years . In 2011 , the pathogen re-emerged with a vengeance . In this study , we compare diverse pa...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "&", "methods" ]
[ "taxonomy", "phylogenetics", "data", "management", "fiber", "crops", "genome", "analysis", "crops", "plants", "flowering", "plants", "bacteria", "genomic", "libraries", "computer", "and", "information", "sciences", "crop", "science", "gene", "expression", "evolutionary"...
2017
Genomics-enabled analysis of the emergent disease cotton bacterial blight
The premotor theory of attention postulates that spatial attention arises from the activation of saccade areas and that the deployment of attention is the consequence of motor programming . Yet attentional and oculomotor processes have been shown to be dissociable at the neuronal level in covert attention tasks . To in...
The premotor theory of attention postulates that spatial visual attention is a consequence of the brain activity that controls eye movement . Indeed , attention and eye movement share overlapping brain networks , and attention is deployed at the target of an eye movement ( saccade ) even before the eyes start to move ....
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cognitive", "neurology", "medicine", "and", "health", "sciences", "reaction", "time", "social", "sciences", "neuropsychology", "neuroscience", "surgical", "and", "invasive", "medical", "procedures", "cognitive", "neuroscience", "cognitive", "psychology", "functional", "e...
2018
Visual attention is not deployed at the endpoint of averaging saccades
Lectin-like bacteriocins consist of tandem monocot mannose-binding domains and display a genus-specific killing activity . Here we show that pyocin L1 , a novel member of this family from Pseudomonas aeruginosa , targets susceptible strains of this species through recognition of the common polysaccharide antigen ( CPA ...
Due to rapidly increasing rates of antibiotic resistance observed among Gram-negative pathogens , such as Pseudomonas aeruginosa , there is an urgent requirement for novel approaches to the treatment of bacterial infections . Lectin-like bacteriocins are highly potent protein antibiotics that display an unusual ability...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacteriology", "microbial", "mutation", "protein", "interactions", "microbiology", "bacterial", "biochemistry", "genome", "sequencing", "protein", "classes", "protein", "structure", "bacterial", "pathogens", "plant", "microbiology", "proteins", "microbial", "pathogens", "b...
2014
Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor
Eukaryotic DNA replication origins differ both in their efficiency and in the characteristic time during S phase when they become active . The biological basis for these differences remains unknown , but they could be a consequence of chromatin structure . The availability of genome-wide maps of nucleosome positions ha...
Eukaryotic DNA replication begins at specific sites in the genome called replication origins , which are bound by the proteins that comprise the origin recognition complex ( ORC ) . In budding yeast , there are more replication origins available than are used in any particular cell division cycle . Each origin has a ch...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry/replication", "and", "repair", "genetics", "and", "genomics/chromosome", "biology" ]
2010
Diversity of Eukaryotic DNA Replication Origins Revealed by Genome-Wide Analysis of Chromatin Structure
Sex chromosomes evolve distinctive types of chromatin from a pair of ancestral autosomes that are usually euchromatic . In Drosophila , the dosage-compensated X becomes enriched for hyperactive chromatin in males ( mediated by H4K16ac ) , while the Y chromosome acquires silencing heterochromatin ( enriched for H3K9me2/...
DNA is packaged with proteins into two general types of chromatin: the transcriptionally active euchromatin and repressive heterochromatin . Sex chromosomes typically evolve from a pair of euchromatic autosomes . The Y chromosome of Drosophila is gene poor and almost entirely heterochromatic; the X chromosome , in cont...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Ancestral Chromatin Configuration Constrains Chromatin Evolution on Differentiating Sex Chromosomes in Drosophila
HIV-1 infection is characterized by a chronic activation of the immune system and suppressed function of T lymphocytes . Regulatory CD4+ CD25high FoxP3+CD127low T cells ( Treg ) play a key role in both conditions . Here , we show that HIV-1 positive patients have a significant increase of Treg-associated expression of ...
HIV-1 infection is characterized by a chronic activation of the immune system . Regulatory T cells ( Treg ) represent a population of lymphocytes that controls inappropriate or exaggerated immune activation induced by pathogens , thereby influencing the outcome of various infections . Several studies have shown that Tr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine" ]
2011
CD39/Adenosine Pathway Is Involved in AIDS Progression
So far , the computational identification of transcription factor binding sites is hampered by the complexity of vertebrate genomes . Here we present an in silico procedure to predict target sites of a transcription factor in complex genomes using its binding site . In a first step sequence , comparison of closely rela...
To establish regulatory gene networks that drive key biological processes is of crucial importance to identify the genes that are directly controlled by transcriptional regulators . Ideally , this can be accomplished by identifying the direct transcription factor binding site in the cis-regulatory regions of the respec...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "developmental", "biology", "chicken", "mammals", "medaka", "in", "vitro", "computational", "biology", "teleost", "fishes", "genetics", "and", "genomics" ]
2007
In Vivo Validation of a Computationally Predicted Conserved Ath5 Target Gene Set
Oropouche ( ORO ) virus , a member of the Simbu serogroup , is one of the few human pathogens in the Orthobunyavirus genus in the family Bunyaviridae . Genetic analyses of ORO-like strains from Iquitos , Peru , identified a novel reassortant containing the S and L segments of ORO virus and the M segment of a novel Simb...
Oropouche ( ORO ) virus is one of the few human pathogens in the Orthobunyavirus genus in the family Bunyaviridae . Phylogenetic analyses of ORO-like strains isolated from febrile patients in Iquitos , Peru , identified a novel ORO reassortant virus , which we named Iquitos ( IQT ) virus based on the location of the is...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "virology", "emerging", "viral", "diseases", "neglected", "tropical", "diseases", "biology", "microbiology", "arboviral", "infections" ]
2011
Iquitos Virus: A Novel Reassortant Orthobunyavirus Associated with Human Illness in Peru
Signaling via pattern recognition receptors ( PRRs ) expressed on professional antigen presenting cells , such as dendritic cells ( DCs ) , is crucial to the fate of engulfed microbes . Among the many PRRs expressed by DCs are Toll-like receptors ( TLRs ) and C-type lectins such as DC-SIGN . DC-SIGN is targeted by seve...
Among the most successful of human microbes are intracellular pathogens . By entering the intracellular milieu , these pathogens are protected from harsh environmental factors in the host , including the humoral and cellular immune responses . Porphyromonas gingivalis is an opportunistic pathogen that colonizes the ora...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Porphyromonas gingivalis Evasion of Autophagy and Intracellular Killing by Human Myeloid Dendritic Cells Involves DC-SIGN-TLR2 Crosstalk
Monocyte phenotype and output changes with age , but why this occurs and how it impacts anti-bacterial immunity are not clear . We found that , in both humans and mice , circulating monocyte phenotype and function was altered with age due to increasing levels of TNF in the circulation that occur as part of the aging pr...
As we age , levels of inflammatory cytokines in the blood and tissues increase . Although this appears to be an inevitable part of aging , it ultimately contributes to declining health . Epidemiological studies indicate that older adults with higher than age-average levels of inflammatory cytokines are at increased ris...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2016
TNF Drives Monocyte Dysfunction with Age and Results in Impaired Anti-pneumococcal Immunity
Zika virus ( ZIKV ) is an emergent threat provoking a worldwide explosive outbreak . Since January 2015 , 41 countries reported autochthonous cases . In Brazil , an increase in Guillain-Barré syndrome and microcephaly cases was linked to ZIKV infections . A recent report describing low experimental transmission efficie...
The American continent has recently been the scene of a devastating epidemic of Zika virus and its severe manifestations , such as microcephaly in newborns and Guillain-Barré Syndrome . Zika virus , first detected in 1947 in Africa , only from 2007 started provoking outbreaks . Zika , dengue and chikungunya viruses are...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "dengue", "virus", "vero", "cells", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "togaviruses", "pathogens", "biological", "cultures", "microbiology", "saliva", "alphaviruses", "viruses", "urine", "chikungunya"...
2016
Isolation of Infective Zika Virus from Urine and Saliva of Patients in Brazil
Mass anthelmintic drug administration is recommended in developing countries to address infection by soil-transmitted helminthiases ( STH ) . We quantified the public health benefit of treatment with mebendazole in eight million Vietnamese children aged 5–14 years from 2006 to 2011 . This was compared to the environmen...
Millions of children from developing countries are infected by soil-transmitted helminthiases ( STH ) , an infection of intestinal worms that cause abdominal pain , bad absorption of nutrients from food and a decrease in the amount of red blood cells . This disease can be treated with anthelmintic medication , such as ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "markov", "models", "helminths", "tropical", "diseases", "hookworms", "parasitic", "diseases", "anemia", "animals", "ascaris", "ascaris", "lumbricoides", "mathematics", "pharmaceutics", "neglected", "tropical", "di...
2018
The public health benefit and burden of mass drug administration programs in Vietnamese schoolchildren: Impact of mebendazole
Mucosal associated invariant T cells ( MAIT ) are innate T lymphocytes that detect a large variety of bacteria and yeasts . This recognition depends on the detection of microbial compounds presented by the evolutionarily conserved major-histocompatibility-complex ( MHC ) class I molecule , MR1 . Here we show that MAIT ...
Human Mucosa-Associated Invariant T cells ( MAIT ) detect microbe-derived compounds presented by the MHC-like molecule , MR1 . These foreign antigens are produced by a wide variety of microbes , including commensal and pathogenic bacteria or yeasts . MAIT cells expend shortly after birth and constitute the major antiba...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
MAIT Cells Detect and Efficiently Lyse Bacterially-Infected Epithelial Cells
The anthrax toxin is a tripartite toxin , where the two enzymatic subunits require the third subunit , the protective antigen ( PA ) , to interact with cells and be escorted to their cytoplasmic targets . PA binds to cells via one of two receptors , TEM8 and CMG2 . Interestingly , the toxin times and triggers its own e...
Bacillus anthracis is the bacterium responsible for the anthrax disease . Its virulence is mainly due to 2 factors , the anthrax toxin and the anti-phagocytic capsule . This toxin is composed of three independent polypeptide chains . Two of these have enzymatic activity and are responsible for the effects of the toxin ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/membranes", "and", "sorting", "microbiology/cellular", "microbiology", "and", "pathogenesis" ]
2010
Endocytosis of the Anthrax Toxin Is Mediated by Clathrin, Actin and Unconventional Adaptors
One of the major mechanisms driving the evolution of all organisms is genomic rearrangement . In hyperthermophilic Archaea of the order Thermococcales , large chromosomal inversions occur so frequently that even closely related genomes are difficult to align . Clearly not resulting from the native homologous recombinat...
Mobile elements ( MEs ) such as viruses , plasmids and transposons infect most living organisms and often encode recombinases promoting their insertion into cellular genomes . These insertions alter the genome of their host according to two main mechanisms . First , MEs provide new functions to the cell by integrating ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "recombination", "reactions", "enzymes", "enzymology", "plasmid", "construction", "genome", "analysis", "genetic", "elements", "dna", "construction", "dna", "molecular", "biology", "techniques", "homologous", "recombination", "research", "and", "analysis", "methods", "seq...
2017
Flipping chromosomes in deep-sea archaea
Human Herpesvirus 6 ( HHV-6 ) is a ubiquitous virus with an estimated seroprevalence of 95% in the adult population . HHV-6 is associated with several neurologic disorders , including multiple sclerosis , an inflammatory demyelinating disease affecting the CNS . Animal models of HHV-6 infection would help clarify its r...
The human herpesviruses HHV-6A and HHV-6B are widely distributed in the human population , but also specifically associated with several central nervous system ( CNS ) diseases . We investigated HHV-6A and HHV-6B infections in the common marmoset , a non-human primate naturally susceptible to infection , unlike rodents...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "marmoset", "viral", "transmission", "and", "infection", "immunology", "microbiology", "neuroscience", "animal", "models", "model", "organisms", "neurovirulence", "neuroimaging", "viral", "disease", "diagnosis", "animal", "models", "of", "infection", "viral", "immune", ...
2013
Novel Marmoset (Callithrix jacchus) Model of Human Herpesvirus 6A and 6B Infections: Immunologic, Virologic and Radiologic Characterization
Motor adaptation paradigms provide a quantitative method to study short-term modification of motor commands . Despite the growing understanding of the role motion states ( e . g . , velocity ) play in this form of motor learning , there is little information on the relative stability of memories based on these movement...
Human motor adaptation of limb movement in response to force perturbations has been shown to be motion-state dependent . That is , the compensatory response to these disturbances is correlated and proportional to the temporal changes in the position , velocity , and acceleration during the motion . Despite a growing un...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "velocity", "learning", "medicine", "and", "health", "sciences", "classical", "mechanics", "perturbation", "(geology)", "social", "sciences", "neuroscience", "learning", "and", "memory", "simulation", "and", "modeling", "cognitive", "psychology", "geology", "evolutionary"...
2017
The decay of motor adaptation to novel movement dynamics reveals an asymmetry in the stability of motion state-dependent learning
The broadly conserved bacterial signalling molecule cyclic-di-adenosine monophosphate ( c-di-AMP ) controls osmoresistance via its regulation of potassium ( K+ ) and compatible solute uptake . High levels of c-di-AMP resulting from inactivation of c-di-AMP phosphodiesterase activity leads to poor growth of bacteria und...
Second messengers relay signals received from the environment to intracellular targets that adjust cellular physiology . One widespread bacterial cyclic-dinucleotide signalling molecule , cyclic-di-AMP ( c-di-AMP ) has been shown to regulate a range of cellular processes via binding to protein and riboswitch targets , ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "deletion", "mutation", "medicine", "and", "health", "sciences", "classical", "mechanics", "chemical", "compounds", "pathology", "and", "laboratory", "medicine", "aliphatic", "amino", "acids", "pathogens", "population", "genetics", "microbiology", "operons", "organic", ...
2018
Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
The rapid proliferation of antibiotic-resistant pathogens has spurred the use of drug combinations to maintain clinical efficacy and combat the evolution of resistance . Drug pairs can interact synergistically or antagonistically , yielding inhibitory effects larger or smaller than expected from the drugs' individual p...
The use of antibiotics against bacterial infections has led to the emergence of multi-drug resistant pathogens such as tuberculosis and MRSA . In order to control resistance , clinicians have increasingly turned to multi-antibiotic therapies . The common wisdom is to use combinations of drugs that act synergistically t...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/population", "genetics", "evolutionary", "biology/microbial", "evolution", "and", "genomics", "ecology/evolutionary", "ecology", "pharmacology/drug", "resistance", "microbiology/microbial", "evolution", "and", "genomics", "computational", "biology/evolutio...
2010
Optimal Drug Synergy in Antimicrobial Treatments
Long noncoding RNAs constitute a major fraction of the eukaryotic transcriptome , and together with proteins , they intricately fine-tune various growth regulatory signals to control cellular homeostasis . Here , we describe the functional characterisation of a novel pair of long intergenic noncoding RNAs ( lincRNAs ) ...
The growth of multicellular organisms is tightly regulated by cellular homeostasis mediated by cell division . This is achieved with the help of various proteins acting in a highly coordinated manner via intricately woven intercellular signalling pathways , which regulate cell division . Here , we identify a long nonco...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "non-coding", "rna", "sequences", "centrosomes", "protein", "interactions", "gene", "regulation", "cancer", "risk", "factors", "long", "non-coding", "rnas", "oncology", "mammalian", "genomics", "medical", "risk", "factors", "ce...
2018
Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins
The adjuvanticity of bacterial adenylate cyclase toxins has been ascribed to their capacity , largely mediated by cAMP , to modulate APC activation , resulting in the expression of Th2–driving cytokines . On the other hand , cAMP has been demonstrated to induce a Th2 bias when present during T cell priming , suggesting...
Colonization by pathogens requires keeping at bay the host immune defenses , at least at the onset of infection . The adenylate cyclase ( AC ) toxins produced by many pathogenic bacteria assist in this crucial function by catalyzing the production of cAMP , which acts as a potent immunosuppressant . Nevertheless , at l...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/cellular", "microbiology", "and", "pathogenesis", "infectious", "diseases", "immunology/immunomodulation", "immunology/immune", "response", "infectious", "diseases/bacterial", "infections", "immunology/immunity", "to", "infections", "immunology/leukocyte", "activation" ]
2009
The Adenylate Cyclase Toxins of Bacillus anthracis and Bordetella pertussis Promote Th2 Cell Development by Shaping T Cell Antigen Receptor Signaling
Nelson Bay orthoreoviruses ( NBVs ) are members of the fusogenic orthoreoviruses and possess 10-segmented double-stranded RNA genomes . NBV was first isolated from a fruit bat in Australia more than 40 years ago , but it was not associated with any disease . However , several NBV strains have been recently identified a...
Nelson Bay orthoreoviruses ( NBVs ) are members of the fusogenic orthoreoviruses that have various host species , including reptiles , birds , and mammals . Recently , several NBV strains have been isolated from patients with acute respiratory tract infections . Isolation of these pathogenic reoviruses raises concerns ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "serum", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "pathogens", "biological", "cultures", "microbiology", "viral", "structure", "reoviruses", "viruses", "rna", "viruses", "forms", "of", "dna", "...
2016
Reverse Genetics for Fusogenic Bat-Borne Orthoreovirus Associated with Acute Respiratory Tract Infections in Humans: Role of Outer Capsid Protein σC in Viral Replication and Pathogenesis
Ribosome biogenesis is a global process required for growth and proliferation of all cells , yet perturbation of ribosome biogenesis during human development often leads to tissue-specific defects termed ribosomopathies . Transcription of the ribosomal RNAs ( rRNAs ) by RNA polymerases ( Pol ) I and III , is considered...
Ribosomes synthesize all proteins , and are therefore critical for cell growth and proliferation . Ribosome biogenesis , or the process of making ribosomes , is one of the most energy consuming processes within a cell , and disruptions in ribosome biogenesis can lead to congenital disorders termed ribosomopathies . Int...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "death", "medicine", "and", "health", "sciences", "cell", "processes", "vertebrates", "animals", "animal", "models", "osteichthyes", "developmental", "biology", "model", "organisms", "embryos", "cartilage", "cellular", "structures", "and", "organelles", "researc...
2016
The Roles of RNA Polymerase I and III Subunits Polr1c and Polr1d in Craniofacial Development and in Zebrafish Models of Treacher Collins Syndrome
Classical and Connectionist theories of cognitive architecture seek to explain systematicity ( i . e . , the property of human cognition whereby cognitive capacity comes in groups of related behaviours ) as a consequence of syntactically and functionally compositional representations , respectively . However , both the...
Our minds are not the sum of some arbitrary collection of mental abilities . Instead , our mental abilities come in groups of related behaviours . This property of human cognition has substantial biological advantage in that the benefits afforded by a cognitive behaviour transfer to a related situation without any of t...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "mathematics", "neuroscience/psychology", "computational", "biology" ]
2010
Categorial Compositionality: A Category Theory Explanation for the Systematicity of Human Cognition
Tomato Yellow Leaf Curl Virus Disease incited by Tomato yellow leaf curl virus ( TYLCV ) causes huge losses in tomato production worldwide and is caused by different related begomovirus species . Breeding for TYLCV resistance has been based on the introgression of multiple resistance genes originating from several wild...
Tomato yellow leaf curl virus and related begomoviruses cause major economic damage to tomato production in tropical and subtropical regions around the world . Because cultivated tomato is inherently susceptible to these viruses , breeders have incorporated several resistance alleles from wild tomato relatives . Among ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "rna", "interference", "plant", "biology", "crop", "genetics", "gene", "function", "plant", "science", "crops", "plant", "pathology", "vegetables", "gene", "expression", "plant", "genetics", "crop", "diseases", "biology", "agriculture", "plant", "path...
2013
The Tomato Yellow Leaf Curl Virus Resistance Genes Ty-1 and Ty-3 Are Allelic and Code for DFDGD-Class RNA–Dependent RNA Polymerases
In developing strategies to control malaria vectors , there is increased interest in biological methods that do not cause instant vector mortality , but have sublethal and lethal effects at different ages and stages in the mosquito life cycle . These techniques , particularly if integrated with other vector control int...
It has recently been proposed that mosquito vectors of malaria may be controlled by biopesticide sprays containing spores of fungi that are pathogenic to mosquitoes , causing reduced blood feeding activity and eventual death . This technique has been shown to have strong potential to reduce malaria transmission rates ,...
[ "Abstract", "Introduction", "Models", "Results", "Discussion" ]
[ "infectious", "diseases", "infectious", "diseases/fungal", "infections", "public", "health", "and", "epidemiology/epidemiology", "public", "health", "and", "epidemiology/infectious", "diseases", "infectious", "diseases/protozoal", "infections", "infectious", "diseases/epidemiolog...
2009
Combining Fungal Biopesticides and Insecticide-Treated Bednets to Enhance Malaria Control
Congenital toxoplasmosis is a serious but preventable and treatable disease . Gestational screening facilitates early detection and treatment of primary acquisition . Thus , fetal infection can be promptly diagnosed and treated and outcomes can be improved . We tested 180 sera with the Toxoplasma ICT IgG-IgM point-of-c...
Toxoplasmosis , a disease caused by the parasite Toxoplasma gondii , presents a major health burden in both the developed and developing world . Untreated congenital toxoplasmosis causes damage to the eye and brain , but early detection and treatment reduce transmission and disease . Fetal infection can be promptly dia...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "maternal", "health", "obstetrics", "and", "gynecology", "toxoplasma", "gondii", "geographical", "locations", "parasitic", "diseases", "parasitic", "protozoans", "pr...
2017
Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries
Although a vaccine could be available as early as 2016 , vector control remains the primary approach used to prevent dengue , the most common and widespread arbovirus of humans worldwide . We reviewed the evidence for effectiveness of vector control methods in reducing its transmission . Studies of any design published...
Dengue fever has increased dramatically over the past 50 years and today is the most widespread mosquito-borne arboviral disease , affecting nearly half the world’s population in 128 countries . Until the arrival of a vaccine , control of its Aedes vectors has been the only method to prevent dengue infection . With den...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "vector-borne", "diseases", "randomized", "controlled", "trials", "animals", "clinical", "medicine", "mathematics", "statistics", "(mathematics)", "pharmacology", "infec...
2016
Is Dengue Vector Control Deficient in Effectiveness or Evidence?: Systematic Review and Meta-analysis
Site-directed mutagenesis combined with binding affinity measurements is widely used to probe the nature of ligand interactions with GPCRs . Such experiments , as well as structure-activity relationships for series of ligands , are usually interpreted with computationally derived models of ligand binding modes . Howeve...
G-protein coupled receptors constitute a family of drug targets of outstanding interest , with more than 30% of the marketed drugs targeting a GPCR . The combination of site-directed mutagenesis , biochemical experiments and computationally generated 3D structural models has traditionally been used to investigate these...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "membrane", "proteins", "biomacromolecule-ligand", "interactions", "biochemistry", "biochemical", "simulations", "transmembrane", "proteins", "cell", "biology", "proteins", "biology", "and", "life", "sciences", "computational", "biology", "cellular", "structures", "and", "o...
2014
Computational Prediction of Alanine Scanning and Ligand Binding Energetics in G-Protein Coupled Receptors
The majority of mammalian microRNA ( miRNA ) genes reside within introns of protein-encoding and non-coding genes , yet the mechanisms coordinating primary transcript processing into both mature miRNA and spliced mRNA are poorly understood . Analysis of melanoma invasion suppressor miR-211 expressed from intron 6 of me...
MicroRNA ( miRNA ) genes are transcribed as long primary RNAs containing local hairpins that are excised by the Microprocessor complex minimally composed of Drosha and DGCR8 . Most mammalian miRNAs reside in introns of protein-encoding and non-coding genes , but it is unclear how microprocessing of an intronic miRNA an...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "genetics", "biology", "molecular", "cell", "biology", "genetics", "and", "genomics" ]
2011
Feed-Forward Microprocessing and Splicing Activities at a MicroRNA–Containing Intron
Soil-transmitted helminth ( STH ) infections are among the most prevalent neglected tropical diseases ( NTD ) worldwide . Since the publication of the WHO road map to combat NTD in 2012 , there has been a renewed commitment to control STH . In this study , we analysed the geographical distribution and effect of communi...
Soil transmitted helminth infections ( hookworms , Trichuris and Ascaris ) are highly prevalent across south Asia and south east Asia and recently several large initiatives have been launched to control or interrupt transmission . We conducted a systematic review of STH infections to identify the communities in south A...
[ "Abstract", "Introduction", "Methods", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "helminths", "population", "dynamics", "tropical", "diseases", "hookworms", "geographical", "locations", "parasitic", "diseases", "animals", "ascaris", "neglected", "tropical", "diseases", "population", "biology", ...
2018
Geographical distribution of soil transmitted helminths and the effects of community type in South Asia and South East Asia – A systematic review
miRNAs are small regulatory RNAs that , due to their considerable potential to target a wide range of mRNAs , are implicated in essentially all biological process , including cancer . miR-10a is particularly interesting considering its conserved location in the Hox cluster of developmental regulators . A role for this ...
Posttranscriptional regulation by microRNA molecules constitutes an important mechanism for gene regulation and numerous studies have demonstrated a correlation between deregulated microRNA levels and diseases , such as cancer . However , genetics studies linking individual microRNAs to the etiology of cancer remain sc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Loss of miR-10a Activates Lpo and Collaborates with Activated Wnt Signaling in Inducing Intestinal Neoplasia in Female Mice
Animals are constantly exposed to the time-varying visual world . Because visual perception is modulated by immediately prior visual experience , visual cortical neurons may register recent visual history into a specific form of offline activity and link it to later visual input . To examine how preceding visual inputs...
Animals are constantly exposed to a visual world that varies over time . To examine how the visual cortex integrates visual information that is temporally spaced , we monitored neuronal activity of the primary visual cortex ( V1 ) using single- and multicell recording techniques . We discovered that a brief visual stim...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Neocortical Rebound Depolarization Enhances Visual Perception
Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential . During development , the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential . In the mouse , mammary stem cells with transplantable regenerat...
Many adult tissues are maintained by a rare subset of undifferentiated stem cells that can self-renew and give rise to specialized daughter cells that have a more limited regenerative ability . The recent identification of cells in the fetal and adult mammary gland that display the properties of stem cells provides a f...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "stem", "cells", "developmental", "biology", "biology", "adult", "stem", "cells" ]
2013
Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells
Circadian clocks have evolved as internal time keeping mechanisms that allow anticipation of daily environmental changes and organization of a daily program of physiological and behavioral rhythms . To better examine the mechanisms underlying circadian clocks in animals and to ask whether clock gene expression and func...
The fruit fly Drosophila melanogaster is an excellent model system for studying the internal circadian clocks that animals use for daily time keeping . Since clocks exist and function in animals not only in adults , but also during prior development , the question arises if and how adult circadian rhythms depend on dev...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "gene", "regulation", "anatomy", "and", "physiology", "neuroscience", "gene", "function", "animal", "models", "physiological", "processes", "developmental", "biology", "drosophila", "melanogaster", "model", "organisms", "organism", "development", "chronobiology", "molecular...
2011
Adult Circadian Behavior in Drosophila Requires Developmental Expression of cycle, But Not period
Down syndrome ( DS ) , commonly caused by an extra copy of chromosome 21 ( chr21 ) , occurs in approximately one out of 700 live births . Precisely how an extra chr21 causes over 80 clinically defined phenotypes is not yet clear . Reduced representation bisulfite sequencing ( RRBS ) analysis at single base resolution r...
Down syndrome ( DS ) occurs in approximately one out of 700 live births . DS is caused by an extra copy of chromosome 21 . Although over 80 clinically defined phenotypes are identified for DS , each affected individual may only show some of the disease phenotypes . Understanding how the extra chromosome 21 causes vario...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "aneuploidy", "dna", "modification", "chromosomal", "disorders", "genetics", "epigenetics", "biology", "human", "genetics", "down", "syndrome" ]
2013
Global DNA Hypermethylation in Down Syndrome Placenta
Dengue virus ( DENV ) is a flavivirus of worldwide importance , with approximately 4 billion people across 128 countries at risk of infection , and up to 390 million infections and 96 million clinically apparent cases estimated annually . Previous in vitro studies have shown that lipids and lipoproteins play a role in ...
Dengue is a viral infection of worldwide importance with up to 96 million cases annually . Cholesterol , a type of lipid , may play a role in dengue virus infectivity and severity , but changes in cholesterol levels over the course of illness are not well-understood . To investigate the relationship between development...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Lower Low-Density Lipoprotein Cholesterol Levels Are Associated with Severe Dengue Outcome
As circulating monocytes enter the site of disease , the local microenvironment instructs their differentiation into tissue macrophages ( MΦ ) . To identify mechanisms that regulate MΦ differentiation , we studied human leprosy as a model , since M1-type antimicrobial MΦ predominate in lesions in the self-limited form ...
Mycobacterial diseases , such as leprosy , continue to be serious causes of mortality and morbidity worldwide . They pose a unique treatment challenge due to their ability to modify the immune response in infected individuals . For example , in leprosy there are two distinct manifestations of the disease , each charact...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "antimicrobials", "biotechnology", "mycobacterium", "leprae", "medicine", "and", "health", "sciences", "immune", "cells", "chemical", "compounds", "small", "molecules", "drugs", "immunology", "tropical", "diseases", "microbiology", "organic", "compounds"...
2016
Jagged1 Instructs Macrophage Differentiation in Leprosy
Chromosome instability ( CIN ) is observed in most solid tumors and is linked to somatic mutations in genome integrity maintenance genes . The spectrum of mutations that cause CIN is only partly known and it is not possible to predict a priori all pathways whose disruption might lead to CIN . To address this issue , we...
Cancer results from mutations that alter the function of normal genes . The results of these mutations directly lead to the known properties of cancer cells , for example , over-proliferation or resistance to cellular death signals . An additional property of most tumors is chromosome instability ( CIN ) —the unequal d...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "gene", "networks", "cancer", "genetics", "genetic", "mutation", "chromosome", "structure", "and", "function", "microbiology", "model", "organisms", "mutation", "types", "chromosome", "biology", "biology", "systems", "biology", "cell", "biology", "genetic", "screens", ...
2011
The Complete Spectrum of Yeast Chromosome Instability Genes Identifies Candidate CIN Cancer Genes and Functional Roles for ASTRA Complex Components
The molecular nature of biological variation is not well understood . Indeed , many questions persist regarding the types of molecular changes and the classes of genes that underlie morphological variation within and among species . Here we have taken a candidate gene approach based on previous mapping results to ident...
Among the goals of modern evolutionary biology is to identify the molecular genetic sources of natural variation . Although genetic mapping has led to an increased understanding of the genetic architecture of natural variation , there are surprisingly few cases where the molecular source of the variation has been ident...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "developmental", "biology", "plant", "science", "plant", "biology", "genetics", "biology", "population", "biology", "evolutionary", "biology", "genetics", "and", "genomics" ]
2011
Natural Allelic Variation Defines a Role for ATMYC1: Trichome Cell Fate Determination
It has been hypothesized that helminth infections increase HIV susceptibility by enhancing systemic immune activation and hence contribute to elevated HIV-1 transmission in sub-Saharan Africa . To study systemic immune activation and HIV-1 co-receptor expression in relation to different helminth infections and in respo...
Helminth infections are common in sub-Saharan Africa where about half of the population may be infected with one or more helminth species . HIV infection is also highly prevalent in this region . Because of the geographic overlap of helminth and HIV infections , it has been hypothesized that helminth infections may inc...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "flow", "cytometry", "medicine", "and", "health", "sciences", "immune", "cells", "cytokines", "immune", "activation", "immunity", "to", "infections", "immunology", "tropical", "diseases", "parasitic", "diseases", "developmental", "biology", "molecular"...
2014
Helminth-Associated Systemic Immune Activation and HIV Co-receptor Expression: Response to Albendazole/Praziquantel Treatment
This study sought understand how the 2014–2016 EVD Virus Disease ( EVD ) outbreak impacted the nutrition sector in Sierra Leone and use findings for improving nutrition responses during future outbreaks of this magnitude . This qualitative study was iterative and emergent . In-depth interviews ( n = 42 ) were conducted...
The 2014–2016 EVD outbreak has greatly impacted the population health and nutrition of affected countries in West Africa , including that of Sierra Leone . Since this recent outbreak , the humanitarian community acknowledges the need for improved solutions to better prepare for , and respond . Despite the importance of...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Conclusions" ]
[ "neonatology", "learning", "children", "medicine", "and", "health", "sciences", "maternal", "health", "geographical", "locations", "social", "sciences", "neuroscience", "learning", "and", "memory", "pediatrics", "age", "groups", "research", "design", "cognitive", "psych...
2019
A qualitative study to understand how Ebola Virus Disease affected nutrition in Sierra Leone—A food value-chain framework for improving future response strategies
Crimean-Congo hemorrhagic fever virus ( CCHFV ) is a zoonotic agent that causes severe , life-threatening disease , with a case fatality rate of 10–50% . It is the most widespread tick-borne virus in the world , with cases reported in Africa , Asia and Eastern Europe . CCHFV is a genetically diverse virus . Its genetic...
Crimean-Congo hemorrhagic fever ( CCHF ) is an acute , tick-borne disease with a case fatality rate of 10–30% . It is geographically the most widespread tick-borne disease in the world . In recent years there has been an increase of the disease incidence in several countries , mainly in the countries of the Balkan . Th...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "viral", "hemorrhagic", "fevers", "infectious", "diseases", "zoonoses", "crimean-congo", "hemorrhagic", "fever", "neglected", "tropical", "diseases", "biology", "microbiology", "evolutionary", "biology", "viral", "diseases" ]
2014
Molecular Epidemiology of Crimean-Congo Hemorrhagic Fever Virus in Kosovo
Old world Zoonotic Cutaneous Leishmaniasis ( ZCL ) is a vector-borne human disease caused by Leishmania major , a unicellular eukaryotic parasite transmitted by pool blood-feeding sand flies mainly to wild rodents , such as Psammomys obesus . The human beings who share the rodent and sand fly habitats can be subverted ...
Old world cutaneous leishmaniasis is a vector-borne disease occurring in rural areas of developing countries . The main reservoirs are the rodents Psammomys obesus and Meriones shawi . Zoonotic Leishmania transmission cycle is maintained in the burrows of rodents where the sand fly Phlebotomus papatasi finds the ideal ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "public", "health", "and", "epidemiology", "environmental", "sciences", "statistics", "mathematics", "biostatistics", "infectious", "diseases", "environmental", "geography", "epidemiology", "biology", "public", "health", "confidence", "intervals", "ecology", "ea...
2012
Temporal Dynamics and Impact of Climate Factors on the Incidence of Zoonotic Cutaneous Leishmaniasis in Central Tunisia
Arenaviridae synthesize viral mRNAs using short capped primers presumably acquired from cellular transcripts by a ‘cap-snatching’ mechanism . Here , we report the crystal structure and functional characterization of the N-terminal 196 residues ( NL1 ) of the L protein from the prototypic arenavirus: lymphocytic choriom...
The Arenaviridae virus family includes several life-threatening human pathogens that cause meningitis or hemorrhagic fever . These RNA viruses replicate and transcribe their genome using an RNA synthesis machinery for which no structural data currently exist . They synthesize viral mRNAs using short capped primers pres...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "virology/viral", "replication", "and", "gene", "regulation", "biophysics/structural", "genomics", "biochemistry/protein", "folding", "virology/emerging", "viral", "diseases" ]
2010
The N-Terminal Domain of the Arenavirus L Protein Is an RNA Endonuclease Essential in mRNA Transcription
Ovulation is essential for the propagation of the species and involves a proteolytic degradation of the follicle wall for the release of the fertilizable oocyte . However , the precise mechanisms for regulating these proteolytic events are largely unknown . Work from our lab and others have shown that there are several...
Ovulation is the process of releasing fertilizable oocytes from the ovary and is essential for metazoan reproduction . Our recent work has demonstrated principles governing ovulation process that are highly conserved across species , such that both mammals and Drosophila utilize matrix metalloproteinase ( Mmp ) to degr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
A Follicle Rupture Assay Reveals an Essential Role for Follicular Adrenergic Signaling in Drosophila Ovulation
Antibiotic treatment of Group A Streptococcus ( GAS ) pharyngitis is important in acute rheumatic fever ( ARF ) prevention , however clinical guidelines for prescription vary . GAS carriers with acute viral infections may receive antibiotics unnecessarily . This review assessed the prevalence of GAS pharyngitis and car...
Treating sore throats caused by Group A Streptococcus infections ( GAS pharyngitis ) with antibiotics is important for preventing acute rheumatic fever ( ARF ) . It is impossible to distinguish patients with true GAS pharyngitis infections from GAS carriers with pharyngitis caused by viral infections when throat swab c...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "antimicrobials", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "drugs", "microbiology", "database", "searching", "group", "a", "streptococcal", "infection", "throat", "bacterial", "diseases", "age", "groups", "streptococcal", "p...
2018
Group A Streptococcus pharyngitis and pharyngeal carriage: A meta-analysis
Calmodulin ( CaM ) is a ubiquitous Ca2+ buffer and second messenger that affects cellular function as diverse as cardiac excitability , synaptic plasticity , and gene transcription . In CA1 pyramidal neurons , CaM regulates two opposing Ca2+-dependent processes that underlie memory formation: long-term potentiation ( L...
Calmodulin is a versatile Ca2+ signal mediator and a buffer in a wide variety of body organs including the heart and brain . In the brain , calmodulin regulates intracellular molecular processes that change the strength of connectivity between neurons , thus contributing to various brain functions including memory form...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "neuroscience/theoretical", "neuroscience", "cell", "biology/cell", "signaling", "biophysics/theory", "and", "simulation", "computational", "biology/computational", "neuroscience", "neuroscience/neuronal", "signaling", "mechanisms", "biochemistry/cell", "signaling", "and", "traffic...
2010
Lobe Specific Ca2+-Calmodulin Nano-Domain in Neuronal Spines: A Single Molecule Level Analysis
Estimates of leptospirosis morbidity identified Oceania as the region with highest burden . Besides Australia and New Zealand , Oceania is home of Pacific Island Countries and Territories , most of which are developing countries facing a number of challenges . Their archipelago geography notably affects health infrastr...
Leptospirosis is thought to impose its highest burden to tropical island populations , especially in the Pacific region of Oceania . Yet , very few information has ever been reported from some of the Pacific Island Countries and Territories , including Vanuatu . In this study , we aimed at evidencing leptospirosis in a...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "geomorphology", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "leptospira", "pathology", "and", "laboratory", "medicine", "landforms", "pathogens", "topography", "tropical", "diseases", "geographical", "locations", "vanuatu", "mi...
2018
High incidence of leptospirosis in an observational study of hospital outpatients in Vanuatu highlights the need for improved awareness and diagnostic capacities
Blood flow and mechanical forces in the ventricle are implicated in cardiac development and trabeculation . However , the mechanisms of mechanotransduction remain elusive . This is due in part to the challenges associated with accurately quantifying mechanical forces in the developing heart . We present a novel computa...
We present a novel computational workflow for quantifying hemodynamic forces in developing zebrafish embryos by coupling high resolution 4-D light sheet imaging with a moving domain blood flow solver . Our framework employs deformable image registration to extract the motion of the ventricle from high resolution image ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "fish", "medicine", "and", "health", "sciences", "body", "fluids", "cardiovascular", "anatomy", "cardiac", "ventricles", "vertebrates", "endocardium", "animals", "animal", "models", "osteichthyes", "developmental", "biology", "viscosity", "model", "organisms", "hemodynami...
2017
A method to quantify mechanobiologic forces during zebrafish cardiac development using 4-D light sheet imaging and computational modeling
The determinants of parasite persistence or elimination after treatment and clinical resolution of cutaneous leishmaniasis ( CL ) are unknown . We investigated clinical and parasitological parameters associated with the presence and viability of Leishmania after treatment and resolution of CL caused by L . Viannia . Se...
Control of cutaneous leishmaniasis ( CL ) in the Americas is dependent upon active case detection and treatment . The efficacy and effectiveness of therapeutic interventions is based on clinical resolution of disease , not on parasitological clearance . The detection of dermotropic Leishmania in tissues such as nasal a...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "parasitology", "respiratory", "system", "protozoans", "signs", "and", "symptoms", "leish...
2017
Clinical and parasitological factors in parasite persistence after treatment and clinical cure of cutaneous leishmaniasis
The evolution of substitutions conferring drug resistance to HIV-1 is both episodic , occurring when patients are on antiretroviral therapy , and strongly directional , with site-specific resistant residues increasing in frequency over time . While methods exist to detect episodic diversifying selection and continuous ...
When exposed to treatment , HIV-1 and other rapidly evolving viruses have the capacity to acquire drug resistance mutations ( DRAMs ) , which limit the efficacy of antivirals . There are a number of experimentally well characterized HIV-1 DRAMs , but many mutations whose roles are not fully understood have also been re...
[ "Abstract", "Introduction", "Models", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "hiv", "evolutionary", "modeling", "biology", "computational", "biology", "viral", "diseases" ]
2012
Modeling HIV-1 Drug Resistance as Episodic Directional Selection
The Huntington’s disease ( HD ) protein , huntingtin ( HTT ) , is a large protein consisting of 3144 amino acids and has conserved N-terminal sequences that are followed by a polyglutamine ( polyQ ) repeat . Loss of Htt is known to cause embryonic lethality in mice , whereas polyQ expansion leads to adult neuronal dege...
The 17 amino acids in the N-terminal region of huntingtin ( HTT ) are conserved in a wide range of species and are followed by a polyglutamine repeat whose expansion causes selective neurodegeneration in Huntington’s disease ( HD ) . Loss of Htt can affect developing neurons and early embryonic development in mice . Wh...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "astrocytes", "neurodegenerative", "diseases", "genetic", "diseases", "brain", "neurites", "neuroscience", "macroglial", "cells", "animal", "models", "developmental", "biology", "model", "organisms", "embryos", "autosomal", "domina...
2016
N-terminal Huntingtin Knock-In Mice: Implications of Removing the N-terminal Region of Huntingtin for Therapy
Gametogenesis is a sexually dimorphic process requiring profound differences in germ cell differentiation between the sexes . In mammals , the presence of heteromorphic sex chromosomes in males creates additional sex-specific challenges , including incomplete X and Y pairing during meiotic prophase . This triggers form...
Genes related to the sexual regulator Doublesex of Drosophila have been found to control sexual development in a wide variety of animals , ranging from roundworms to mammals . In this paper , we investigate the function of the Dmrt7 gene , one of seven related genes in the mouse . Female mammals are XX and males are XY...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "meiosis", "developmental", "biology", "gametogenesis", "dm", "domain", "dmrt7", "xy", "body" ]
2007
A Mammal-Specific Doublesex Homolog Associates with Male Sex Chromatin and Is Required for Male Meiosis