Search is not available for this dataset
article
stringlengths
4.36k
149k
summary
stringlengths
32
3.35k
section_headings
listlengths
1
91
keywords
listlengths
0
141
year
stringclasses
13 values
title
stringlengths
20
281
The mainstay of current schistosomiasis control programs is mass preventive chemotherapy of school-aged children with praziquantel . This treatment is delivered through school-based , community-based , or combined school- and community-based systems . Attaining very high coverage rates for children is essential in mass...
Schistosomiasis is a chronic inflammatory condition , caused by parasitic flukes , that affects over 290 million people worldwide . Consequences of infection include anemia , stunted growth , liver abnormalities , and subfertility . Currently , the main approach to schistosomiasis control involves mass preventive treat...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "children", "medicine", "and", "health", "sciences", "education", "sociology", "tropical", "diseases", "social", "sciences", "teachers", "parasitic", "diseases", "health", "care", "age", "groups", "pharmaceutics", "neglected", "tropical", "diseases", "pharmacology", "fa...
2017
Systematic review of community-based, school-based, and combined delivery modes for reaching school-aged children in mass drug administration programs for schistosomiasis
Recent work has shown that much of the missing heritability of complex traits can be resolved by estimates of heritability explained by all genotyped SNPs . However , it is currently unknown how much heritability is missing due to poor tagging or additional causal variants at known GWAS loci . Here , we use variance co...
Heritable diseases have an unknown underlying “genetic architecture” that defines the distribution of effect-sizes for disease-causing mutations . Understanding this genetic architecture is an important first step in designing disease-mapping studies , and many theories have been developed on the nature of this distrib...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2013
Quantifying Missing Heritability at Known GWAS Loci
Voltage-gated cation channels regulate neuronal excitability through selective ion flux . NALCN , a member of a protein family that is structurally related to the α1 subunits of voltage-gated sodium/calcium channels , was recently shown to regulate the resting membrane potentials by mediating sodium leak and the firing...
Neurons communicate to their targets through synapses that are activated by the electrical signals conveyed along neuronal processes . The tightly regulated ion flux across the cell membrane drives the generation of these electrical signals; it is therefore important to identify ion channels that regulate the excitabil...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience", "developmental", "biology" ]
2008
A Putative Cation Channel, NCA-1, and a Novel Protein, UNC-80, Transmit Neuronal Activity in C. elegans
The V3 loop of the HIV-1 Env protein is the primary determinant of viral coreceptor usage , whereas the V1V2 loop region is thought to influence coreceptor binding and participate in shielding of neutralization-sensitive regions of the Env glycoprotein gp120 from antibody responses . The functional properties and antig...
The HIV envelope gene ( env ) encodes viral surface proteins ( Env ) that are vital to the basic processes used by the virus to infect and cause disease in humans . Adaptations in env determine which cells the virus can infect , and permit the virus to avoid elimination by the immune system . Env is one of the most var...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "evolutionary", "biology/microbial", "evolution", "and", "genomics", "infectious", "diseases", "infectious", "diseases/sexually", "transmitted", "diseases", "virology/virus", "evolution", "and", "symbiosis", "virology/virion", "structure,", "assembly,", "and", "egress", "viro...
2010
HIV-1 Envelope Subregion Length Variation during Disease Progression
A potent therapeutic T-cell vaccine may be an alternative treatment of chronic hepatitis B virus ( HBV ) infection . Previously , we developed a DNA prime-adenovirus ( AdV ) boost vaccination protocol that could elicit strong and specific CD8+ T-cell responses to woodchuck hepatitis virus ( WHV ) core antigen ( WHcAg )...
Chronic hepatitis B virus ( HBV ) infection is one of the major causes of liver cirrhosis and liver cancer worldwide . Recommended treatment regimens of chronic hepatitis B based on interferon alpha and nucleot ( s ) ide analogues do not lead to the satisfactory results . Over the last 20 years , continuous efforts hav...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "clinical", "immunology", "immunology", "biology", "microbiology" ]
2013
Combination of DNA Prime – Adenovirus Boost Immunization with Entecavir Elicits Sustained Control of Chronic Hepatitis B in the Woodchuck Model
Changes in gene expression play an important role in evolution , yet the molecular mechanisms underlying regulatory evolution are poorly understood . Here we compare genome-wide binding of the six transcription factors that initiate segmentation along the anterior-posterior axis in embryos of two closely related specie...
The differentiation of cells , tissues , and organs during animal development is established by a process in which genes that control cell identity and behavior are turned on and off at specific times and places . This process is choreographed , to a large extent , by a collection of proteins known as transcription fac...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genetics", "and", "genomics/comparative", "genomics", "genetics", "and", "genomics/functional", "genomics", "computational", "biology/comparative", "sequence", "analysis", "developmental", "biology/developmental", "evolution", "computational", "biology/evolutionary", "modeling", ...
2010
Binding Site Turnover Produces Pervasive Quantitative Changes in Transcription Factor Binding between Closely Related Drosophila Species
Staphylococcus aureus is a devastating mammalian pathogen for which the development of new therapeutic approaches is urgently needed due to the prevalence of antibiotic resistance . During infection pathogens must overcome the dual threats of host-imposed manganese starvation , termed nutritional immunity , and the oxi...
During infection , pathogens must overcome the restriction of essential nutrients such as manganese by the host , while simultaneously coping with other host defenses such as the oxidative burst . Using the host protein that limits manganese availability during infection and mice lacking this effector , we determined t...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "manganese", "oxidative", "stress", "enzymes", "pathogens", "microbiology", "enzymology", "dismutases", "staphylococcus", "aureus", "bacterial", "diseases", "nutrition", "bacteria", "ma...
2017
A Superoxide Dismutase Capable of Functioning with Iron or Manganese Promotes the Resistance of Staphylococcus aureus to Calprotectin and Nutritional Immunity
Over two-thirds of the world's population lives in regions where rabies is endemic , resulting in over 15 million people receiving multi-dose post-exposure prophylaxis ( PEP ) and over 55 , 000 deaths per year globally . A major goal in rabies virus ( RABV ) research is to develop a single-dose PEP that would simplify ...
Over two-thirds of the world's population lives in regions where rabies is endemic , resulting in over 15 million people receiving post-exposure treatment . A person , disproportionately a child , dies of rabies every 20 minutes and the cost of rabies prevention exceeds $1 billion US dollars per year . The development ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "humoral", "immunity", "medicine", "viral", "vaccines", "immune", "cells", "emerging", "viral", "diseases", "immunity", "to", "infections", "immunology", "microbiology", "host-pathogen", "interaction", "emerging", "infectious", "diseases", "global", "health", "immunizatio...
2013
Investigating the Role for IL-21 in Rabies Virus Vaccine-induced Immunity
We have performed a metabolite quantitative trait locus ( mQTL ) study of the 1H nuclear magnetic resonance spectroscopy ( 1H NMR ) metabolome in humans , building on recent targeted knowledge of genetic drivers of metabolic regulation . Urine and plasma samples were collected from two cohorts of individuals of Europea...
Physiological concentrations of metabolites—small molecules involved in biochemical processes in living systems—can be measured and used to diagnose and predict disease states . A common goal is to detect and clinically exploit statistical differences in metabolite concentrations between diseased and healthy individual...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "public", "health", "and", "epidemiology", "mathematics", "epidemiology", "statistics", "genetics", "chemistry", "biology", "genomics", "biostatistics", "genetics", "and", "genomics", "genetic", "epidemiology" ]
2011
A Genome-Wide Metabolic QTL Analysis in Europeans Implicates Two Loci Shaped by Recent Positive Selection
Hybridization between species is an important mechanism for the origin of novel lineages and adaptation to new environments . Increased allelic variation and modification of the transcriptional network are the two recognized forces currently deemed to be responsible for the phenotypic properties seen in hybrids . Howev...
The Saccharomyces cerevisiae “sensu stricto” group represent an excellent example of closely related species which can readily hybridise to occupy new ecological niches . Hybrids harbour the DNA of both parents and can display diverse pattern of gene expression . Less is known about the protein interactions that occur ...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Chimeric Protein Complexes in Hybrid Species Generate Novel Phenotypes
Almost all attention and learning—in particular , most early learning—take place in social settings . But little is known of how our brains support dynamic social interactions . We recorded dual electroencephalography ( EEG ) from 12-month-old infants and parents during solo play and joint play . During solo play , flu...
We are a social species . Most infants and young children spend the majority of their early waking hours in the company of others . However , almost everything that we know about how the brain subserves early attention and learning comes from studies that examined brain function in one individual at a time just because...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "learning", "children", "medicine", "and", "health", "sciences", "sociology", "brain", "electrophysiology", "social", "sciences", "electrophysiology", "neuroscience", "learning", "and", "memory", "social", "systems", "age", "groups", "clinical", "medicine", "cognitive", ...
2018
Parental neural responsivity to infants’ visual attention: How mature brains influence immature brains during social interaction
Predictions of interactions between target proteins and potential leads are of great benefit in the drug discovery process . We present a comprehensively applicable statistical prediction method for interactions between any proteins and chemical compounds , which requires only protein sequence data and chemical structu...
This work describes a statistical method that identifies chemical compounds binding to a target protein given the sequence of the target or distinguishes proteins to which a small molecule binds given the chemical structure of the molecule . As our method can be utilized for virtual screening that seeks for lead compou...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "chemical", "biology", "mathematics/statistics", "pharmacology/drug", "development", "computational", "biology" ]
2009
Integrating Statistical Predictions and Experimental Verifications for Enhancing Protein-Chemical Interaction Predictions in Virtual Screening
M . tuberculosis N-acetyl-glucosamine-1-phosphate uridyltransferase ( GlmUMtb ) is a bi-functional enzyme engaged in the synthesis of two metabolic intermediates N-acetylglucosamine-1-phosphate ( GlcNAc-1-P ) and UDP-GlcNAc , catalyzed by the C- and N-terminal domains respectively . UDP-GlcNAc is a key metabolite essen...
The synthesis of the Mtb cell wall involves a cascade of reactions catalyzed by cytosolic and cell membrane-bound enzymes . The reaction catalyzed by GlmUMtb ( an enzyme with acetyltransferase and uridyltransferase activities ) generates UDP-GlcNAc , a central nucleotide-sugar building block of the cell wall . Apart fr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Depletion of M. tuberculosis GlmU from Infected Murine Lungs Effects the Clearance of the Pathogen
In budding yeast , the major regulator of the mitotic exit network ( MEN ) is Tem1 , a GTPase , which is inhibited by the GTPase-activating protein ( GAP ) , Bfa1/Bub2 . Asymmetric Bfa1 localization to the bud-directed spindle pole body ( SPB ) during metaphase also controls mitotic exit , but the molecular mechanism a...
During mitosis the replicated chromosomes are distributed equally to the daughter cells . Once the chromosomes have segregated properly , a pathway called the mitotic exit network ( MEN ) becomes activated to complete mitosis . How MEN activation is coordinated with segregation of the chromosomes is currently a focus o...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "mitosis", "model", "organisms", "molecular", "cell", "biology", "cell", "division", "cell", "biology", "gene", "regulation", "genetics", "molecular", "genetics", "biology", "yeast", "and", "fungal", "models", "saccharomyces", "cerevisiae", "microbiology", "genetics", ...
2012
Cdc5-Dependent Asymmetric Localization of Bfa1 Fine-Tunes Timely Mitotic Exit
Inhibition of human papillomavirus ( HPV ) replication is a promising therapeutic approach for intervening with HPV-related pathologies . Primary targets for interference are two viral proteins , E1 and E2 , which are required for HPV replication . Both E1 and E2 are phosphoproteins; thus , the protein kinases that pho...
Human papillomaviruses ( HPVs ) are small DNA viruses that infect epithelial cells and can cause a variety of hyperplastic changes in the infected tissue . Replication of the HPV genomes relies largely on the host cell factors . The only viral proteins that are necessary for the virus replication are E1 and E2 . Relati...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "phosphorylation", "urology", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "hpv-11", "microbiology", "viruses", "dna", "replication", "dna", "viruses", "sexually", "transmitted", "diseases", "h...
2019
Activity of CK2α protein kinase is required for efficient replication of some HPV types
The acceleration of the control of soil-transmitted helminth ( STH ) infections in Nigeria , emphasizing preventive chemotherapy , has become imperative in light of the global fight against neglected tropical diseases . Predictive risk maps are an important tool to guide and support control activities . STH infection p...
Infections with three kinds of parasitic worms—hookworm , roundworm , and whipworm—are collectively known as soil-transmitted helminths ( STHs ) . These parasitic worm infections are widespread in Nigeria , but the exact distribution is poorly understood . In view of the global commitment to control STH infections , th...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Bayesian Geostatistical Model-Based Estimates of Soil-Transmitted Helminth Infection in Nigeria, Including Annual Deworming Requirements
Metrics of phylogenetic tree reliability , such as parametric bootstrap percentages or Bayesian posterior probabilities , represent internal measures of the topological reproducibility of a phylogenetic tree , while the recently introduced aLRT ( approximate likelihood ratio test ) assesses the likelihood that a branch...
The construction of phylogenetic trees , which depict past relationships between groups of DNA or protein sequences , has valuable application in many fields of study , most commonly evolutionary and population biology . Before drawing conclusions from phylogenetic trees , it is important to assess how accurate those r...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology", "none", "computational", "biology" ]
2007
Measures of Clade Confidence Do Not Correlate with Accuracy of Phylogenetic Trees
The hepatic circadian clock plays a key role in the daily regulation of glucose metabolism , but the precise molecular mechanisms that coordinate these two biological processes are not fully understood . In this study , we identify a novel connection between the regulation of RORγ by the clock machinery and the diurnal...
The circadian clock plays a critical role in the regulation of many physiological processes , including metabolism and energy homeostasis . The retinoic acid-related orphan receptor γ ( RORγ ) functions as a ligand-dependent transcription factor that regulates transcription by binding as a monomer to ROR-responsive ele...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "carbohydrate", "metabolism", "body", "weight", "medicine", "and", "health", "sciences", "functional", "genomics", "diabetic", "endocrinology", "gene", "regulation", "regulatory", "proteins", "dna", "transcription", "diabetes", "mellitus", "physiological", "parameters", "...
2014
Retinoic Acid-Related Orphan Receptor γ (RORγ): A Novel Participant in the Diurnal Regulation of Hepatic Gluconeogenesis and Insulin Sensitivity
The mismatch negativity ( MMN ) is an event related potential evoked by violations of regularity . Here , we present a model of the underlying neuronal dynamics based upon the idea that auditory cortex continuously updates a generative model to predict its sensory inputs . The MMN is then modelled as the superposition ...
Computational neuroimaging enables quantitative inferences from non-invasive measures of brain activity on the underlying mechanisms . Ultimately , we would like to understand these mechanisms not only in terms of physiology but also in terms of computation . So far , this has not been addressed by mathematical models ...
[ "Abstract", "Introduction", "Models", "Results", "Discussion" ]
[]
2013
A Neurocomputational Model of the Mismatch Negativity
Beyond Mycobacterium ulcerans—specific therapy , sound general wound management is required for successful management of Buruli ulcer ( BU ) patients which places them among the large and diverse group of patients in poor countries with a broken skin barrier . Clinically BU suspicious patients were enrolled between Oct...
Buruli ulcer ( BU ) , currently endemic in more than 30 countries , particularly in West and Central Africa , causes chronic necrotising disease of the skin and subcutaneous soft tissue . The task is twofold , specific therapy directed against the causative agent Mycobacterium ulcerans and general wound management to g...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion", "Conclusion" ]
[ "rheumatology", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "drugs", "tropical", "diseases", "osteomyelitis", "physiological", "processes", "bacterial", "diseases", "signs", "and", "symptoms", "ulcers", "analgesics", "neglected",...
2017
Assessing and managing wounds of Buruli ulcer patients at the primary and secondary health care levels in Ghana
Wild mammals serve as reservoirs for a variety of microbes and play an important role in the enzootic cycles of these microbes . Some of them are vector-borne bacteria in the genera Anaplasma , Ehrlichia and Rickettsia of the order Rickettsiales , which can cause febrile illnesses in human beings as well as animals . A...
In this study , the 16S rDNA-targeted metagenomic sequencing was used to determine the bacterial community and diversity in the spleens of small wild mammals from China . The 16S rDNAs were amplified from the spleen genomic DNAs of 35 small wild mice and shrews and sequenced by Illumina MiSeq technology . More than 1 ,...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "geomorphology", "medicine", "and", "health", "sciences", "immune", "physiology", "microbiome", "pathology", "and", "laboratory", "medicine", "landforms", "shrews", "spleen", "pathogens", "topography", "microbiology", "vertebrates", "rickettsia", "animals", "mammals", "ri...
2018
The spleen microbiota of small wild mammals reveals distinct patterns with tick-borne bacteria
The Epstein-Barr virus ( EBV ) nuclear antigen leader protein ( EBNA-LP ) is the first viral latency-associated protein produced after EBV infection of resting B cells . Its role in B cell transformation is poorly defined , but it has been reported to enhance gene activation by the EBV protein EBNA2 in vitro . We gener...
Epstein-Barr virus ( EBV ) infects almost everyone . Once infected , people harbor the virus for life , shedding it in saliva . Infection of children is asymptomatic , but a first infection during adolescence or adulthood can cause glandular fever ( infectious mononucleosis ) . EBV is also implicated in several differe...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "body", "fluids", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "regulatory", "proteins", "immunology", "microbiology", "dna-binding", "proteins", "dna", "...
2018
Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome
Gastrointestinal infection is often associated with hypophagia and weight loss; however , the precise mechanisms governing these responses remain poorly defined . Furthermore , the possibility that alterations in feeding during infection may be beneficial to the host requires further study . We used the nematode Trichi...
Infection with intestinal parasites often results in a period of reduced appetite which can result in weight loss; however the factors which control these feeding alterations and the reason why they occur is unknown . We used the nematode parasite Trichinella spiralis , which during its life cycle causes intestinal and...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "small", "intestine", "immune", "physiology", "immune", "cells", "cytokines", "immunologic", "subspecialties", "immunity", "to", "infections", "immunology", "anatomy", "and", "physiology", "parasitic", "diseases", "helminth", "infection", "adaptive", "immunity...
2013
Adaptive Immunity Alters Distinct Host Feeding Pathways during Nematode Induced Inflammation, a Novel Mechanism in Parasite Expulsion
Yeast Npl3 is a highly abundant , nuclear-cytoplasmic shuttling , RNA-binding protein , related to metazoan SR proteins . Reported functions of Npl3 include transcription elongation , splicing and RNA 3’ end processing . We used UV crosslinking and analysis of cDNA ( CRAC ) to map precise RNA binding sites , and strand...
Npl3 is a yeast mRNA binding protein with many reported functions in RNA processing . We wanted to identify direct targets and therefore combined analyses of the transcriptome-wide effects of the loss of Npl3 on gene expression with UV crosslinking and bioinformatics to identify RNA-binding sites for Npl3 . We found th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Loss of the Yeast SR Protein Npl3 Alters Gene Expression Due to Transcription Readthrough
The epistatic interactions that underlie evolutionary constraint have mainly been studied for constant external conditions . However , environmental changes may modulate epistasis and hence affect genetic constraints . Here we investigate genetic constraints in the adaptive evolution of a novel regulatory function in v...
Epistatic interactions limit the number of adaptive trajectories to peaks on evolutionary fitness landscapes , and may therefore hamper the progress of evolution . Recent research has focused on adaptive landscapes in one constant environment . However , adaptive evolution is generally known to occur in variable , hete...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "physics", "systems", "biology", "biochemistry", "ecology", "genetics", "biology", "computational", "biology", "evolutionary", "biology", "microbiology", "biophysics" ]
2013
Environmental Dependence of Genetic Constraint
The parasite Leishmania often relies on gene rearrangements to survive stressful environments . However , safeguarding a minimum level of genome integrity is important for cell survival . We hypothesized that maintenance of genomic integrity in Leishmania would imply a leading role of the MRE11 and RAD50 proteins consi...
The parasite Leishmania relies on gene rearrangements to survive stressful conditions . However , maintaining a minimum level of genomic integrity is crucial for cell survival . Studies in other organisms have provided evidence that the DNA repair proteins MRE11 and RAD50 are involved in chromosomes organization , prot...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "Methods" ]
[ "cell", "hybridization", "molecular", "probe", "techniques", "biological", "cultures", "parasitic", "protozoans", "microhomology-mediated", "end", "joining", "protozoans", "leishmania", "molecular", "biology", "techniques", "translocations", "dna", "research", "and", "analy...
2016
Chromosomal Translocations in the Parasite Leishmania by a MRE11/RAD50-Independent Microhomology-Mediated End Joining Mechanism
Pathogens causing acute fever , with the exception of malaria , remain largely unidentified in sub-Saharan Africa , given the local unavailability of diagnostic tests and the broad differential diagnosis . We conducted a cross-sectional study including outpatient acute undifferentiated fever in both children and adults...
Malaria remains one of the most important causes of fever in sub-Saharan Africa . However , its share is declining , since the diagnosis and treatment of malaria have improved significantly over the years . Hence leading to an increase in the number of patients presenting with non-malarial fever . Often , obvious clini...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "dengue", "virus", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "togaviruses", "chikungunya", "infection", "pathogens", "immunology", "tropical", "diseases", "microbiology", ...
2019
Dengue and chikungunya among outpatients with acute undifferentiated fever in Kinshasa, Democratic Republic of Congo: A cross-sectional study
Pitch is one of the most important features of natural sounds , underlying the perception of melody in music and prosody in speech . However , the temporal dynamics of pitch processing are still poorly understood . Previous studies suggest that the auditory system uses a wide range of time scales to integrate pitch-rel...
Pitch is one of the most important features of natural sounds . The pitch sensation depends strongly on its temporal context , as happens , for example , in the perception of melody in music and prosody in speech . However , the temporal dynamics of pitch processing are poorly understood . Perceptual studies have shown...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "otolaryngology/audiology", "computational", "biology/computational", "neuroscience" ]
2009
Understanding Pitch Perception as a Hierarchical Process with Top-Down Modulation
Nelfinavir is a potent HIV-protease inhibitor with pleiotropic effects in cancer cells . Experimental studies connect its anti-cancer effects to the suppression of the Akt signaling pathway , but the actual molecular targets remain unknown . Using a structural proteome-wide off-target pipeline , which integrates molecu...
The traditional approach to drug discovery of “one drug – one target – one disease” is insufficient , especially for complex diseases , like cancer . This inadequacy is partially addressed by accepting the notion of polypharmacology – one drug is likely to bind to multiple targets with varying affinity . However , to i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "medicine", "molecular", "dynamics", "drugs", "and", "devices", "pharmacology", "chemistry", "biology", "drug", "discovery", "systems", "biology", "biochemistry", "drug", "research", "and", "development", "computational", "chemistry", "computational", "bi...
2011
Drug Discovery Using Chemical Systems Biology: Weak Inhibition of Multiple Kinases May Contribute to the Anti-Cancer Effect of Nelfinavir
Most natural odors have sparse molecular composition . This makes the principles of compressed sensing potentially relevant to the structure of the olfactory code . Yet , the largely feedforward organization of the olfactory system precludes reconstruction using standard compressed sensing algorithms . To resolve this ...
Many olfactory systems are capable of accurately sensing a minimum of thousands of different odorants using as few as hundreds of different receptors . This compression raises the possibility that the mathematical properties of compressed sensing might be relevant to olfaction , similar to how these properties were fou...
[ "Abstract", "Introduction", "Models", "and", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "brain", "vertebrates", "neuroscience", "animals", "animal", "models", "optimization", "drosophila", "melanogaster", "model", "organisms", "mathematics", "odorants", "materials", "science", "drosophila", "research", ...
2016
A Robust Feedforward Model of the Olfactory System
Trypanosoma brucei belongs to a group of unicellular , flagellated parasites that are responsible for human African trypanosomiasis . An essential aspect of parasite pathogenicity is cytoskeleton remodelling , which occurs during the life cycle of the parasite and is accompanied by major changes in morphology and organ...
Trypanosoma brucei is the parasite responsible for human African trypanosomiasis , a disease also known as sleeping sickness . African trypanosomiasis is present in Sub-Saharan Africa and transmitted by infected tsetse flies . This devastating disease is lethal if untreated , making it important to understand and chara...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "rna", "interference", "pathology", "and", "laboratory", "medicine", "microtubules", "pathogens", "parasitic", "protozoans", "trypanosoma", "brucei", "protozoans", "epigenetics", "immunologic", "techniques", "cellular", "structures",...
2017
Interaction between the flagellar pocket collar and the hook complex via a novel microtubule-binding protein in Trypanosoma brucei
Protein-protein interactions ( PPIs ) formed between short linear motifs and globular domains play important roles in many regulatory and signaling processes but are highly underrepresented in current protein-protein interaction databases . These types of interactions are usually characterized by a specific binding mot...
Fine-tuning of many cellular processes relies on weak , transient protein-protein interactions . Such interactions often involve compact functional modules , called short linear motifs ( SLiMs ) that can bind to specific globular domains . SLiM-mediated interactions can carry out diverse molecular functions by targetin...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "protein", "interactions", "protein", "interaction", "networks", "dyneins", "molecular", "motors", "network", "analysis", "sequence", "motif", "analysis", "research", "and", "analysis", "methods", "sequence", "analysis", "computer", "and", "information", "sciences", "ne...
2017
Novel linear motif filtering protocol reveals the role of the LC8 dynein light chain in the Hippo pathway
Mathematical models have been used successfully at diverse scales of biological organization , ranging from ecology and population dynamics to stochastic reaction events occurring between individual molecules in single cells . Generally , many biological processes unfold across multiple scales , with mutations being th...
Mathematical understanding of how randomness at the molecular scale , also known as molecular noise , ultimately affects the fate of organisms and whole populations is widely recognized as a challenging problem in multi-scale modeling . Here , we develop an analytical framework for analyzing how the randomness of indiv...
[ "Abstract", "Introduction", "Methods", "and", "results", "Discussion" ]
[ "bacteriology", "organismal", "evolution", "innate", "immune", "system", "ecology", "and", "environmental", "sciences", "medicine", "and", "health", "sciences", "bacteriophages", "immunology", "microbiology", "viruses", "methylation", "microbial", "evolution", "theoretical"...
2019
Molecular noise of innate immunity shapes bacteria-phage ecologies
Trachoma is a disease that can lead to visual impairment and ultimately blindness . Previous estimates of health losses from trachoma using the Global Burden of Disease methodology have not , however , included the stage prior to visual impairment . We estimated the burden of all stages of trachoma in South Sudan and a...
Trachoma is an infectious disease that is endemic to the Republic of South Sudan . In the absence of appropriate treatment recurrent re-infection in an individual will lead to progressively severe states of trachoma , eventually leading to the loss of visual acuity and finally blindness . Here we distinguish between th...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "public", "health", "and", "epidemiology", "epidemiology", "global", "health", "neglected", "tropical", "diseases" ]
2012
The Burden of Trachoma in South Sudan: Assessing the Health Losses from a Condition of Graded Severity
Toxocariasis is a worldwide helminthic zoonosis caused by infection with the larvae of the ascarid worms that comprise the Toxocara spp . Children are particularly prone to infection because they are exposed to the eggs in sandboxes and playgrounds contaminated with dog and cat feces . Certain behaviors , such as a geo...
Human toxocariasis is a neglected parasitic infection occurring in Brazil and worldwide . The combination of the close proximity of canines and felines to humans , environmental contamination by infectious forms of the parasite and neglect by public health officials provides a favorable situation for the spread of this...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Conclusions" ]
[ "public", "and", "occupational", "health", "infectious", "diseases", "medicine", "and", "health", "sciences", "epidemiology", "pediatrics" ]
2014
Seroprevalence and Modifiable Risk Factors for Toxocara spp. in Brazilian Schoolchildren
Natural products have moved into the spotlight as possible sources for new drugs in the treatment of helminth infections including schistosomiasis . Surprisingly , insect-derived compounds have largely been neglected so far in the search for novel anthelminthics , despite the generally recognized high potential of inse...
Natural compounds represent one of the richest sources for the discovery of new active compounds against diseases such as cancer or infections , including helminth infections that cause the highest disease burden in tropical countries . Surprisingly , insects have been almost completely neglected with respect to anthel...
[ "Abstract", "Introduction", "Materials", "&", "methods", "Results", "Discussion" ]
[ "schistosoma", "invertebrates", "schistosoma", "mansoni", "medicine", "and", "health", "sciences", "alkaloids", "reproductive", "system", "chemical", "compounds", "helminths", "light", "microscopy", "animals", "confocal", "laser", "microscopy", "microscopy", "confocal", "...
2019
Insects in anthelminthics research: Lady beetle-derived harmonine affects survival, reproduction and stem cell proliferation of Schistosoma mansoni
Widely used chemical genetic screens have greatly facilitated the identification of many antiviral agents . However , the regions of interaction and inhibitory mechanisms of many therapeutic candidates have yet to be elucidated . Previous chemical screens identified Daclatasvir ( BMS-790052 ) as a potent nonstructural ...
The emergence of drug resistance during antiviral treatment limits treatment options and poses challenges to pharmaceutical development . Meanwhile , the search for novel antiviral compounds with chemical genetic screens has led to the identification of antiviral agents with undefined drug mechanisms . Daclatasvir , an...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "antimicrobials", "organismal", "evolution", "microbial", "evolution", "virology", "microbial", "control", "biology", "and", "life", "sciences", "microbiology", "antivirals", "evolutionary", "biology", "viral", "evolution" ]
2014
A Quantitative High-Resolution Genetic Profile Rapidly Identifies Sequence Determinants of Hepatitis C Viral Fitness and Drug Sensitivity
Zoonotic pathogens respond to changes in host range and/or pathogen , vector and host ecology . Environmental changes ( biodiversity , habitat changes , variability in climate ) , even at a local level , lead to variability in environmental pathogen dynamics and can facilitate their transmission from natural reservoirs...
Many emerging pathogens are zoonotic and transmit from their abiotic reservoir to wild animals , domesticated animals and humans . It is now well known that environmental changes lead to variability in their dynamics in the environment and contribute to changes in the infectious risk . Many aquatic bacteria are respons...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "leptospira", "pathology", "and", "laboratory", "medicine", "pathogens", "tropical", "diseases", "microbiology", "seasons", "bacterial", "diseases", "urban", "environments", "geology",...
2019
Comparison of Mycobacterium ulcerans (Buruli ulcer) and Leptospira sp. (Leptospirosis) dynamics in urban and rural settings
The gambiense form of sleeping sickness is a neglected tropical disease , which is presumed to be anthroponotic . However , the parasite persists in human populations at levels of considerable rarity and as such the existence of animal reservoirs has been posited . Clarifying the impact of animal host reservoirs on the...
Sleeping sickness , a disease that strikes predominantly poor populations in sub-Saharan Africa , has been targeted for elimination as a public health problem . Despite decades of control operations the disease remains enigmatic and is capable of persisting in populations at low levels of prevalence . Two mechanisms ar...
[ "Abstract", "Introduction", "Discussion", "Methods" ]
[]
2015
Implications of Heterogeneous Biting Exposure and Animal Hosts on Trypanosomiasis brucei gambiense Transmission and Control
TAR-DNA-binding protein-43 ( TDP-43 ) C-terminus encodes a prion-like domain widely presented in RNA-binding proteins , which functions to form dynamic oligomers and also , amazingly , hosts most amyotrophic lateral sclerosis ( ALS ) -causing mutations . Here , as facilitated by our previous discovery , by circular dic...
Amyotrophic lateral sclerosis ( ALS ) is the most prevalent fatal motor neuron disease . It was identified ~140 years ago , but the exact mechanism underlying the disease has still not been well defined . TAR-DNA-binding protein-43 ( TDP-43 ) was identified as the major component of the proteinaceous inclusions present...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2016
ALS-Causing Mutations Significantly Perturb the Self-Assembly and Interaction with Nucleic Acid of the Intrinsically Disordered Prion-Like Domain of TDP-43
There is increasing evidence that genetic risk variants for non-syndromic cleft lip/palate ( nsCL/P ) are also associated with normal-range variation in facial morphology . However , previous analyses are mostly limited to candidate SNPs and findings have not been consistently replicated . Here , we used polygenic risk...
Non-syndromic cleft lip/palate ( nsCL/P ) is a birth defect , primarily affecting the upper lip and hard palate . Individuals with nsCL/P and their unaffected family members sometimes present with other minor craniofacial anomalies and may have differences in facial morphology compared to the general population . Here ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "genome-wide", "association", "studies", "medicine", "and", "health", "sciences", "face", "computational", "biology", "developmental", "biology", "mathematics", "statistics", "(mathematics)", "genome", "analysis", "cleft", "palate", "molecular", "genetics", "morphogenesis",...
2018
Investigating the shared genetics of non-syndromic cleft lip/palate and facial morphology
The evolutionary divergence of mitochondrial ribosomes from their bacterial and cytoplasmic ancestors has resulted in reduced RNA content and the acquisition of mitochondria-specific proteins . The mitochondrial ribosomal protein of the small subunit 34 ( MRPS34 ) is a mitochondria-specific ribosomal protein found only...
Mitochondria make most of the energy required by eukaryotic cells and therefore they are essential for their normal function and survival . Mitochondrial function is regulated by both the mitochondrial and nuclear genome . Mutations in nuclear genes encoding mitochondrial proteins lead to mitochondrial dysfunction and ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction
Bunyamwera orthobunyavirus is both the prototype and study model of the Bunyaviridae family . The viral NSs protein seems to contribute to the different outcomes of infection in mammalian and mosquito cell lines . However , only limited information is available on the growth of Bunyamwera virus in cultured mosquito cel...
Bunyamwera and serologically related viruses are widely distributed in tropical and sub-tropical regions , and cause febrile illness in man . The viruses possess a trisegmented genome and can evolve by genetic reassortment generating viruses with different pathogenicity , like Ngari virus , a reassortant between Bunyam...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "virology", "biology", "microbiology" ]
2012
Role of Bunyamwera Orthobunyavirus NSs Protein in Infection of Mosquito Cells
Comparative research on food web structure has revealed generalities in trophic organization , produced simple models , and allowed assessment of robustness to species loss . These studies have mostly focused on free-living species . Recent research has suggested that inclusion of parasites alters structure . We assess...
Food webs are networks of feeding interactions among species . Although parasites comprise a large proportion of species diversity , they have generally been underrepresented in food web data and analyses . Previous analyses of the few datasets that contain parasites have indicated that their inclusion alters network s...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "community", "ecology", "ecology", "food", "web", "structure", "coastal", "ecology", "biology", "microbiology", "marine", "biology", "parasitology" ]
2013
Parasites Affect Food Web Structure Primarily through Increased Diversity and Complexity
Brucellosis is a highly contagious zoonosis affecting livestock and human beings . The human disease lacks pathognomonic symptoms and laboratory tests are essential for its diagnosis . However , most tests are difficult to implement in the areas and countries were brucellosis is endemic . Here , we compared the simple ...
The Rose Bengal Test ( RBT ) for brucellosis serological diagnosis was adapted to test serum dilutions and its usefulness evaluated using sera of Brucella culture positive patients , persons with contact with Brucella but no symptoms , veterinarians accidentally injected with vaccine Rev 1 who had not developed the dis...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/bacterial", "infections", "microbiology/medical", "microbiology" ]
2011
The Rose Bengal Test in Human Brucellosis: A Neglected Test for the Diagnosis of a Neglected Disease
Groups can make precise collective estimations in cases like the weight of an object or the number of items in a volume . However , in others tasks , for example those requiring memory or mental calculation , subjects often give estimations with large deviations from factual values . Allowing members of the group to co...
We modelled how humans interact , and used the models to find strategies that can make groups more accurate . Each individual in a group combines private and public information to make estimations . But when the public information is biased , social information has the effect of making groups agree even more on an inco...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Improving Collective Estimations Using Resistance to Social Influence
Juvenile dermatomyositis ( JDM ) is a chronic inflammatory myopathy and vasculopathy driven by genetic and environmental influences . Here , we investigated the genetic underpinnings of an analogous , spontaneous disease of dogs also termed dermatomyositis ( DMS ) . As in JDM , we observed a significant association wit...
Juvenile dermatomyositis ( JDM ) is an autoimmune disease of the skin and muscle influenced by both genetic and environmental components . Although genes independent of the MHC are thought to contribute to disease pathogenesis , their identification has been complicated by a paucity of biological samples , disease hete...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Methods" ]
[ "genome-wide", "association", "studies", "medicine", "and", "health", "sciences", "variant", "genotypes", "immunology", "vertebrates", "dogs", "animals", "genetic", "mapping", "mammals", "clinical", "medicine", "genome", "analysis", "mammalian", "genomics", "major", "hi...
2017
Beyond the MHC: A canine model of dermatomyositis shows a complex pattern of genetic risk involving novel loci
The replacement of histone H2A with its variant forms is critical for regulating all aspects of genome organisation and function . The histone variant H2A . B appeared late in evolution and is most highly expressed in the testis followed by the brain in mammals . This raises the question of what new function ( s ) H2A ...
The substitution of core histones with their non-allelic variant forms plays a particular important role in regulating chromatin function because they can directly alter the structure of chromatin , and provide new protein interaction interfaces for the recruitment of proteins involved in gene expression . Despite bein...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "sequencing", "techniques", "dna-binding", "proteins", "dna", "transcription", "germ", "cells", "epigenetics", "molecular", "biology", "techniques", "rna", "sequencing", "chromatin", "sperm", "research", "and", "analysis", "methods", "spermatids", "chromosome", "biology",...
2017
A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B
Spatial regulation is often encountered as a component of multi-tiered regulatory systems in eukaryotes , where processes are readily segregated by organelle boundaries . Well-characterized examples of spatial regulation are less common in bacteria . Low-fidelity DNA polymerase V ( UmuD′2C ) is produced in Escherichia ...
Escherichia coli , and many other bacteria , respond to high levels of DNA damage with an inducible system called the SOS response . In this response , bacteria first try to restart replication using non-mutagenic DNA repair strategies . If that fails , replication can be restored using DNA polymerases that simply repl...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[]
2015
Regulation of Mutagenic DNA Polymerase V Activation in Space and Time
Rabies is endemic in Sri Lanka , but little is known about the temporal and spatial trends of rabies in this country . Knowing these trends may provide insight into past control efforts and serve as the basis for future control measures . In this study , we analyzed distribution of rabies in humans and animals over a p...
Rabies is a public health concern in Sri Lanka . The incidence of dog rabies remains unchanged , but the incidence of suspect human rabies is decreasing gradually in Sri Lanka . This finding indicates the effects of improved access to postexposure prophylaxis by animal bite victims and increased rabies awareness . As i...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "disease", "surveillance", "infectious", "diseases", "rabies", "plant", "science", "veterinary", "diseases", "zoonoses", "medicine", "and", "health", "sciences", "epidemiology", "disease", "surveillance", "plant", "pathology", "biology", "and", "life", "sc...
2014
Twelve Years of Rabies Surveillance in Sri Lanka, 1999–2010
The intracellular accommodation structures formed by plant cells to host arbuscular mycorrhiza fungi and biotrophic hyphal pathogens are cytologically similar . Therefore we investigated whether these interactions build on an overlapping genetic framework . In legumes , the malectin-like domain leucine-rich repeat rece...
Our work reveals genetic commonalities between biotrophic intracellular interactions with symbiotic and pathogenic hyphal microbes . The majority of land plants engages in arbuscular mycorrhiza ( AM ) symbiosis with phosphate-acquiring arbuscular mycorrhizal fungi to avoid phosphate starvation . Nutrient exchange in th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "anatomy", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "intracellular", "pathogens", "fungal", "genetics", "symbiosis", "pathogens", "brassica", "fungal", "structure", "plant", "science", "model", "organisms", "experim...
2019
A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis
The infection cycle of viruses creates many opportunities for the exchange of genetic material with the host . Many viruses integrate their sequences into the genome of their host for replication . These processes may lead to the virus acquisition of host sequences . Such sequences are prone to accumulation of mutation...
Many studies focused on the exchange of genetic material between viruses and cellular hosts . The diversity of viruses argues that , along the evolutionary history , viruses have shaped the host genomes . While most viruses have many opportunities to exchange genetic material with their hosts , tracing such events is c...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "genomics", "virology", "viral", "classification", "immunology", "biology", "computational", "biology", "evolutionary", "biology", "microbiology", "evolutionary", "processes", "proteomics", "genetics", "and", "genomics" ]
2012
Viral Proteins Acquired from a Host Converge to Simplified Domain Architectures
In the tropics , the utilization of insecticides is still an important strategy for controlling Aedes aegypti , the principle vector of dengue , chikungunya and Zika viruses . However , increasing insecticide resistance in Ae . aegypti populations might hinder insecticide efficacy on a long-term basis . It will be impo...
Among the pathogens transmitted by Aedes aegypti , dengue virus is the most important due to the number of people affected or at risk and the high rate of mortality worldwide . The confirmation that Ae . aegypti is also the vector of Zika , chikungunya and urban yellow fever poses serious consequences for public health...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "larvicides", "invertebrates", "medicine", "and", "health", "sciences", "geographical", "locations", "enzymology", "animals", "developmental", "biology", "mutation", "substitution", "mutation", "pest", "control", "enzyme", "metabolism", "infectious", "disease", "control", ...
2018
The impact of insecticide applications on the dynamics of resistance: The case of four Aedes aegypti populations from different Brazilian regions
Podoconiosis is an environmental lymphoedema affecting people living and working barefoot on irritant red clay soil . Podoconiosis is relatively well described in southern Ethiopia , but remains neglected in other parts of the Ethiopian highlands . This study aimed to assess the burden of podoconiosis in rural communit...
Podoconiosis is a chronic non-infectious disease resulting in below-knee swelling of the legs in bare-footed people living in red clay soil areas . It is an important and yet neglected problem in tropical Africa , central and south America , and north India . Podoconiosis can be prevented by consistently wearing shoes ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "public", "health", "and", "epidemiology", "primary", "care", "epidemiology", "global", "health", "public", "health" ]
2011
Burden of Podoconiosis in Poor Rural Communities in Gulliso woreda, West Ethiopia
The increase in availability of whole genome sequences makes it possible to search for evidence of adaptation at an unprecedented scale . Despite recent progress , our understanding of the adaptive process is still very limited due to the difficulties in linking adaptive mutations to their phenotypic effects . In this ...
Given the predictions of future environmental fluctuations , it is crucial to understand how organisms adapt to changing environments . The fruit fly Drosophila melanogaster is an ideal model organism to study environmental adaptation because of our deep understanding of developmental , physiological , and metabolic ne...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "biology", "and", "life", "sciences", "population", "biology", "evolutionary", "biology", "molecular", "biology" ]
2014
A Transposable Element Insertion Confers Xenobiotic Resistance in Drosophila
Apart from sharing common ancestry with chordates , sea cucumbers exhibit a unique morphology and exceptional regenerative capacity . Here we present the complete genome sequence of an economically important sea cucumber , A . japonicus , generated using Illumina and PacBio platforms , to achieve an assembly of approxi...
Echinoderms , ubiquitous in the marine environment , are important from evolutionary , ecological , and socioeconomic perspectives . Together with chordates and hemichordates , they form the deuterostome clade , making them a crucial node in the study of chordate ancestry . Within echinoderms , class Holothuroidea is u...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "invertebrates", "methods", "and", "resources", "genomic", "library", "construction", "animals", "invertebrate", "genomics", "genome", "analysis", "dna", "construction", "molecular", "biology", "techniques", "echinoderms", "starfish", "research", "and", "analysis", "metho...
2017
The sea cucumber genome provides insights into morphological evolution and visceral regeneration
During meiosis , chromosomes undergo DNA double-strand breaks ( DSBs ) , which can be repaired using a homologous chromosome to produce crossovers . Meiotic recombination frequency is variable along chromosomes and tends to concentrate in narrow hotspots . We mapped crossover hotspots located in the Arabidopsis thalian...
Sexually reproducing plants and animals produce gametes with half the number of chromosomes , which can participate in fertilization . A specialized cell division called meiosis generates gametes , where the chromosomes are copied once and segregated twice . A further key feature of meiosis is that chromosomes physical...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "methods" ]
[ "meiosis", "plant", "anatomy", "cell", "cycle", "and", "cell", "division", "population", "genetics", "brassica", "cell", "processes", "pollen", "plant", "science", "model", "organisms", "experimental", "organism", "systems", "dna", "molecular", "biology", "techniques"...
2018
Interhomolog polymorphism shapes meiotic crossover within the Arabidopsis RAC1 and RPP13 disease resistance genes
Although evolutionary transitions from sexual to asexual reproduction are frequent in eukaryotes , the genetic bases of these shifts remain largely elusive . Here , we used classic quantitative trait analysis , combined with genomic and transcriptomic information to dissect the genetic basis of asexual , parthenogeneti...
Asexual reproduction is widespread among all major clades of eukaryotes . Parthenogenesis represents a specific mode of asexual reproduction , secondarily derived from sexual reproduction , and refers to the development of a multicellular organism from an unfertilised gamete . Parthenogenesis has evolved independently ...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "methods" ]
[ "quantitative", "trait", "loci", "alleles", "sequence", "assembly", "tools", "developmental", "biology", "genome", "analysis", "molecular", "biology", "techniques", "plants", "research", "and", "analysis", "methods", "sex", "chromosomes", "genomics", "gene", "mapping", ...
2019
A key role for sex chromosomes in the regulation of parthenogenesis in the brown alga Ectocarpus
Rates of random , spontaneous mutation can vary plastically , dependent upon the environment . Such plasticity affects evolutionary trajectories and may be adaptive . We recently identified an inverse plastic association between mutation rate and population density at 1 locus in 1 species of bacterium . It is unknown h...
Spontaneous mutations fuel evolution , but the rate at which they occur can vary for a particular organism depending on its environment—a phenomenon known as mutation-rate plasticity . For microbes growing in liquid , the density to which a population can grow is a key feature of the environment . We find that organism...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "taxonomy", "organismal", "evolution", "microbial", "mutation", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "microbiology", "pseudomonas", "aeruginosa", "fungi", "phylogenetics", "model", "organisms", "data", "manage...
2017
Spontaneous mutation rate is a plastic trait associated with population density across domains of life
Axon pathfinding and synapse formation rely on precise spatiotemporal localization of guidance receptors . However , little is known about the neuron-specific intracellular trafficking mechanisms that underlie the sorting and activity of these receptors . Here we show that loss of the neuron-specific v-ATPase subunit a...
Brain wiring is determined by genetic and environmental factors , nature and nurture . The Drosophila brain is a model for the genetic basis of brain wiring . The fly visual system in particular is thought to be “hard-wired , ” i . e . , encoded solely by a genetic program . Some key genes encode the guidance receptors...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/neuronal", "and", "glial", "cell", "biology" ]
2010
Guidance Receptor Degradation Is Required for Neuronal Connectivity in the Drosophila Nervous System
Accurate gene or protein function prediction is a key challenge in the post-genome era . Most current methods perform well on molecular function prediction , but struggle to provide useful annotations relating to biological process functions due to the limited power of sequence-based features in that functional domain ...
Despite painstaking experimental efforts and the extensive sequence similarity based annotation transfers , less than a half of the fruit fly protein sequences in UniProtKB have some functional annotation . To help fill in this gap , we test the usefulness of publicly available temporal gene expression profiles and the...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[]
2017
Analysis of temporal transcription expression profiles reveal links between protein function and developmental stages of Drosophila melanogaster
The Gram-positive , spore-forming bacterium Paenibacillus larvae is the etiological agent of American Foulbrood ( AFB ) , a globally occurring , deathly epizootic of honey bee brood . AFB outbreaks are predominantly caused by two genotypes of P . larvae , ERIC I and ERIC II , with P . larvae ERIC II being the more viru...
Paenibacillus larvae is the most devastating bacterial pathogen of honey bees . However , the molecular interactions between infected larvae and P . larvae are poorly understood and little more than speculation exist concerning virulence factors . Recently , a putative S-layer protein has been identified in P . larvae ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "veterinary", "diseases", "model", "organisms", "veterinary", "microbiology", "biology", "microbiology", "veterinary", "science" ]
2012
Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
In recent years , an increased focus has been placed upon the possibility of the elimination of soil-transmitted helminth ( STH ) transmission using various interventions including mass drug administration . The primary diagnostic tool recommended by the WHO is the detection of STH eggs in stool using the Kato-Katz ( K...
Soil-transmitted helminths are categorised as a neglected tropical disease and comprise four dominant species ( two hookworms , Trichuris trichuria & Ascaris lumbricoides ) that affect the poorest people in the world . The World Health Organisation ( WHO ) has made great strides in reducing the morbidity induced by STH...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "helminths", "tropical", "diseases", "hookworms", "parasitic", "diseases", "animals", "health", "care", "simulation", "and", "modeling", "ascaris", "neglected", "tropical", "disease...
2018
Testing for soil-transmitted helminth transmission elimination: Analysing the impact of the sensitivity of different diagnostic tools
In many biological settings , two or more cells come into physical contact to form a cell-cell interface . In some cases , the cell-cell contact must be transient , forming on timescales of seconds . One example is offered by the T cell , an immune cell which must attach to the surface of other cells in order to deciph...
The elastohydrodynamics of water in and around cells is playing an increasingly recognized role in biology . In this work , we investigate the flow of extracellular fluid in between cells during the formation of a cell-cell contact , to determine whether its necessary evacuation as the cells approach is a rate-limiting...
[ "Abstract", "Introduction", "Results" ]
[ "classical", "mechanics", "fluid", "mechanics", "permeability", "viscosity", "developmental", "biology", "membrane", "receptor", "signaling", "materials", "science", "molecular", "development", "materials", "physics", "cellular", "structures", "and", "organelles", "chemical...
2019
Hydrodynamics of transient cell-cell contact: The role of membrane permeability and active protrusion length
Periplasmic binding proteins ( PBPs ) are a large family of molecular transporters that play a key role in nutrient uptake and chemotaxis in Gram-negative bacteria . All PBPs have characteristic two-domain architecture with a central interdomain ligand-binding cleft . Upon binding to their respective ligands , PBPs und...
Maltose-binding protein ( MBP ) is a bacterial protein involved in nutrient uptake . Crystallographic studies have revealed two stable conformations: a ligand-free open form and a liganded closed form . The interconversion between the 2 forms has been traditionally viewed as a ligand “induced” process . However , recen...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biotechnology", "biomacromolecule-ligand", "interactions", "molecular", "dynamics", "classical", "mechanics", "statistical", "mechanics", "gibbs", "free", "energy", "enthalpy", "biological", "systems", "engineering", "newton's", "laws", "of", "motion", "protein", "structur...
2011
Accessing a Hidden Conformation of the Maltose Binding Protein Using Accelerated Molecular Dynamics
Paramyxoviruses can establish persistent infections both in vitro and in vivo , some of which lead to chronic disease . However , little is known about the molecular events that contribute to the establishment of persistent infections by RNA viruses . Using parainfluenza virus type 5 ( PIV5 ) as a model we show that ph...
As well as causing acute infections that result in mild to serious disease , many RNA viruses can establish prolonged or persistent infections in some infected individuals , that occasionally lead to chronic or reactive disease . Little is known about the molecular mechanisms involved in the establishment of such infec...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "sequencing", "techniques", "medicine", "and", "health", "sciences", "respiratory", "infections", "rna", "extraction", "microbiology", "pulmonology", "molecular", "biology", "techniques", "rna", "sequencing", "extraction", "techniques", "microbial", "geno...
2019
The switch between acute and persistent paramyxovirus infection caused by single amino acid substitutions in the RNA polymerase P subunit
The Government of Senegal has embarked several years ago on a project that aims to eradicate Glossina palpalis gambiensis from the Niayes area . The removal of the animal trypanosomosis would allow the development more efficient livestock production systems . The project was implemented using an area-wide integrated pe...
The Government of Senegal has embarked since several years on a project that aims to create a tsetse-free area in the Niayes . The project was implemented using an area-wide integrated pest management ( AW-IPM ) strategy where the sterile flies used for the sterile insect technique ( SIT ) component were derived from a...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "sequencing", "techniques", "invertebrates", "medicine", "and", "health", "sciences", "geographical", "locations", "pigments", "animals", "glossina", "organisms", "insect", "pests", "materials", "science", "pest", "control", "molecular", "biology", "techniques", "tsetse",...
2016
A Molecular Method to Discriminate between Mass-Reared Sterile and Wild Tsetse Flies during Eradication Programmes That Have a Sterile Insect Technique Component
Experimental studies show that human pain sensitivity varies across the 24-hour day , with the lowest sensitivity usually occurring during the afternoon . Patients suffering from neuropathic pain , or nerve damage , experience an inversion in the daily modulation of pain sensitivity , with the highest sensitivity usual...
Human pain sensitivity follows a daily ( ∼24 hour ) rhythm . In particular , humans experience the highest sensitivity to pain in the middle of night and lowest in the afternoon . Patients suffering from neuropathy , a disease resulting from nerve damage leading to an increase in pain sensitivity , experience an approx...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "somatosensory", "system", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "nervous", "system", "neuroscience", "signs", "and", "symptoms", "nerve", "fibers", "interneurons", "neuropathic", "pain", "sensory", "physiology", "spinal"...
2019
Modeling the daily rhythm of human pain processing in the dorsal horn
The blood cancer T cell large granular lymphocyte ( T-LGL ) leukemia is a chronic disease characterized by a clonal proliferation of cytotoxic T cells . As no curative therapy is yet known for this disease , identification of potential therapeutic targets is of immense importance . In this paper , we perform a comprehe...
T-LGL leukemia is a blood cancer characterized by an abnormal increase in the abundance of a type of white blood cell called T cell . Since there is no known curative therapy for this disease , identification of potential therapeutic targets is of utmost importance . Experimental identification of manipulations capable...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biology", "computational", "biology", "signaling", "networks" ]
2011
Dynamical and Structural Analysis of a T Cell Survival Network Identifies Novel Candidate Therapeutic Targets for Large Granular Lymphocyte Leukemia
Typhoid fever is endemic in Fiji , with high reported annual incidence . We sought to identify the sources and modes of transmission of typhoid fever in Fiji with the aim to inform disease control . We identified and surveyed patients with blood culture-confirmed typhoid fever from January 2014 through January 2017 . F...
Modeling suggests that Oceania has surpassed Asia and sub-Saharan Africa as the region with the highest typhoid fever incidence . While Pacific Islands are often neglected due to small population sizes , there is an urgent need to understand the epidemiology of typhoid fever in the region . Fiji , an upper-middle incom...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "surface", "water", "body", "fluids", "pathology", "and", "laboratory", "medicine", "pathogens", "geographical", "locations", "microbiology", "salmonella", "typhi", "health", "care", "bacterial", "diseases", "signs", "and", "sy...
2018
Epidemiology and risk factors for typhoid fever in Central Division, Fiji, 2014–2017: A case-control study
After germination , plants enter juvenile vegetative phase and then transition to an adult vegetative phase before producing reproductive structures . The character and timing of the juvenile-to-adult transition vary widely between species . In annual plants , this transition occurs soon after germination and usually i...
The existence of discrete juvenile and adult phases of vegetative development in plants was first recognized in trees , in which these phases are usually prolonged and quite stable . Annual plants also undergo changes in vegetative morphology during shoot development , but the relationship between this process and vege...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "plant", "science", "plant", "biology", "genetics", "biology", "evolutionary", "biology", "genetics", "and", "genomics" ]
2011
MiRNA Control of Vegetative Phase Change in Trees
The activity of trans-membrane proteins such as ion channels is the essence of neuronal transmission . The currently most accurate method for determining ion channel kinetic mechanisms is single-channel recording and analysis . Yet , the limitations and complexities in interpreting single-channel recordings discourage ...
Voltage-gated ion channels affect neuronal integration of information . Some neurons express more than ten different types of voltage-gated ion channels , making information processing a highly convoluted process . Kinetic modelling of ion channels is an important method for unravelling the role of each channel type in...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "physiology", "neuroscience", "rattus", "(rat)", "computational", "biology" ]
2007
A Numerical Approach to Ion Channel Modelling Using Whole-Cell Voltage-Clamp Recordings and a Genetic Algorithm
One goal of modern day neuroscience is the establishment of molecular maps that assign unique features to individual neuron types . Such maps provide important starting points for neuron classification , for functional analysis , and for developmental studies aimed at defining the molecular mechanisms of neuron identit...
Maps of gene expression patterns in nervous systems provide an important resource for neuron classification , for functional analysis , and for developmental studies that ask how different neurons acquire their unique identities . By analyzing transgenic GFP reporter strains , we describe here the expression pattern of...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "methods", "and", "resources", "nervous", "system", "caenorhabditis", "social", "sciences", "reporter", "genes", "neuroscience", "animals", "animal", "models", "caenorhabditis", "elegans", "model", "organisms", "...
2018
An atlas of Caenorhabditis elegans chemoreceptor expression
During meiosis , the rapid movement of telomeres along the nuclear envelope ( NE ) facilitates pairing/synapsis of homologous chromosomes . In mammals , the mechanical properties of chromosome movement and the cytoskeletal structures responsible for it remain poorly understood . Here , applying an in vivo electroporati...
Meiosis is a special type of cell division for gametogenesis , errors in which cause several genetic disorders such as infertility and Down syndrome . In meiotic prophase I , chromosomes are tethered to the nuclear envelope ( NE ) through telomeres , and move rapidly along the NE to get homologs aligned and juxtaposed ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "and", "life", "sciences", "research", "and", "analysis", "methods" ]
2014
The Dissection of Meiotic Chromosome Movement in Mice Using an In Vivo Electroporation Technique
The proteomes of cells , tissues , and organisms reflect active cellular processes and change continuously in response to intracellular and extracellular cues . Deep , quantitative profiling of the proteome , especially if combined with mRNA and metabolite measurements , should provide an unprecedented view of cell sta...
The development of next-generation DNA and RNA sequencing methods has transformed biology , with current platforms generating >1 billion sequencing reads per run . Unfortunately , no method of similar scale and throughput exists to identify and quantify specific proteins in complex mixtures , representing a critical bo...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[]
2015
A Theoretical Justification for Single Molecule Peptide Sequencing
Our work focuses on the stability , resilience , and response to perturbation of the bacterial communities in the human gut . Informative flash flood-like disturbances that eliminate most gastrointestinal biomass can be induced using a clinically-relevant iso-osmotic agent . We designed and executed such a disturbance ...
Complex dynamics of microbial communities underlie their essential roles in health and disease . To maintain or restore healthy states , we must better understand the nature and basis of stability in the gut microbiota , under normal and perturbed conditions . Stability , resilience , and response to perturbation are c...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "taxonomy", "medicine", "and", "health", "sciences", "gut", "bacteria", "pathology", "and", "laboratory", "medicine", "bacteroides", "diarrhea", "phylogenetics", "data", "management", "metagenomics", "signs", "and", "symptoms", "phylogenetic", "analysis", "gastroenterolog...
2017
Multidomain analyses of a longitudinal human microbiome intestinal cleanout perturbation experiment
The coordinated action of a variety of virulence factors allows Salmonella enterica to invade epithelial cells and penetrate the mucosal barrier . The influence of the age-dependent maturation of the mucosal barrier for microbial pathogenesis has not been investigated . Here , we analyzed Salmonella infection of neonat...
Non-typhoidal Salmonella are among of the most prevalent causative agents of infectious diarrheal disease worldwide but also very significantly contribute to infant sepsis and meningitis particularly in developing countries . The underlying mechanisms of the elevated susceptibility of the infant host to systemic Salmon...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacterial", "diseases", "infectious", "diseases", "infectious", "disease", "immunology", "medicine", "and", "health", "sciences", "pediatric", "gastroenterology", "clinical", "immunology", "medical", "microbiology", "gastroenterology", "and", "hepatology", "microbial", "pa...
2014
Age-Dependent Enterocyte Invasion and Microcolony Formation by Salmonella
Helminth parasites are an assemblage of two major phyla of nematodes ( also known as roundworms ) and platyhelminths ( also called flatworms ) . These parasites are a major human health burden , and infections caused by helminths are considered under neglected tropical diseases ( NTDs ) . These infections are typified ...
The fungal metabolite cladosporin is a potent and selective inhibitor of the malaria parasite protein translation machinery enzyme lysyl-tRNA synthetase ( KRS ) . Our computational annotations of parasitic aaRSs from Loa loa and Schistosoma mansoni provide catalogs of these enzymes that drive parasitic protein translat...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "sequencing", "techniques", "schistosoma", "invertebrates", "schistosoma", "mansoni", "crystal", "structure", "helminths", "condensed", "matter", "physics", "animals", "parasitic", "protozoans", "acylation", "protozoans", "sequence", "motif", "analysis", "molecular", "biolo...
2016
Protein Translation Enzyme lysyl-tRNA Synthetase Presents a New Target for Drug Development against Causative Agents of Loiasis and Schistosomiasis
The host vasculature is believed to constitute the principal route of dissemination of Neisseria meningitidis ( Nm ) throughout the body , resulting in septicaemia and meningitis in susceptible humans . In vitro , the Nm outer membrane protein Opc can enhance cellular entry and exit , utilising serum factors to anchor ...
Neisseria meningitidis is a human pathogen that can cross the natural cellular barriers to reach the blood and the brain , causing septicaemia and meningitis . One of its surface molecules , Opc , has the capacity to attach to human cells lining the blood vessels . In vitro , the bacterium can do this by coating itself...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/bacterial", "infections", "cell", "biology/cell", "adhesion", "microbiology/cellular", "microbiology", "and", "pathogenesis" ]
2010
Neisseria meningitidis Opc Invasin Binds to the Sulphated Tyrosines of Activated Vitronectin to Attach to and Invade Human Brain Endothelial Cells
Proteins do not carry out their functions alone . Instead , they often act by participating in macromolecular complexes and play different functional roles depending on the other members of the complex . It is therefore interesting to identify co-complex relationships . Although protein complexes can be identified in a...
Many proteins perform their jobs as part of multi-protein units called complexes , and several technologies exist to identify these complexes and their components with varying precision and throughput . In this work , we describe and apply a computational framework for combining a variety of experimental data to identi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "computational", "biology" ]
2008
Predicting Co-Complexed Protein Pairs from Heterogeneous Data
The nuclear lamina is the structural scaffold of the nuclear envelope and is well known for its central role in nuclear organization and maintaining nuclear stability and shape . In the past , a number of severe human disorders have been identified to be associated with mutations in lamins . Extensive research on this ...
Diploid germ line cells have to undergo meiosis to produce haploid gametes . Haploidization involves pairing and recombination of homologous chromosomes as a prerequisite for their proper segregation . Pairing of homologous chromosomes requires their active repositioning within meiotic nuclei , which depends on the int...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "meiosis", "cellular", "structures", "gene", "function", "germ", "cells", "animal", "models", "model", "organisms", "cell", "nucleus", "chromosome", "biology", "biology", "mouse", "cell", "biology", "genetics", "cellular", "types", "molecular", "cell", "biology", "g...
2013
The Meiotic Nuclear Lamina Regulates Chromosome Dynamics and Promotes Efficient Homologous Recombination in the Mouse
Distributions of the backbone dihedral angles of proteins have been studied for over 40 years . While many statistical analyses have been presented , only a handful of probability densities are publicly available for use in structure validation and structure prediction methods . The available distributions differ in a ...
The three-dimensional structure of a protein enables it to perform its specific function , which may be catalysis , DNA binding , cell signaling , maintaining cell shape and structure , or one of many other functions . Predicting the structures of proteins is an important goal of computational biology . One way of doin...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "mathematics/statistics", "computational", "biology/protein", "structure", "prediction" ]
2010
Neighbor-Dependent Ramachandran Probability Distributions of Amino Acids Developed from a Hierarchical Dirichlet Process Model
Despite its century-old use , the interpretation of local field potentials ( LFPs ) , the low-frequency part of electrical signals recorded in the brain , is still debated . In cortex the LFP appears to mainly stem from transmembrane neuronal currents following synaptic input , and obvious questions regarding the ‘loca...
The first recording of electrical potential from brain activity was reported already in 1875 , but still the interpretation of the signal is debated . To take full advantage of the new generation of microelectrodes with hundreds or even thousands of electrode contacts , an accurate quantitative link between what is mea...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "physics", "cellular", "neuroscience", "neuronal", "morphology", "neurophysiology", "central", "nervous", "system", "biophysic", "al", "simulations", "computational", "neuroscience", "electric", "field", "electricity", "biology", "computational", "biology", "biophysics", "s...
2013
Frequency Dependence of Signal Power and Spatial Reach of the Local Field Potential
p53-signaling is modulated by viruses to establish a host cellular environment advantageous for their propagation . The Epstein-Barr virus ( EBV ) lytic program induces phosphorylation of p53 , which prevents interaction with MDM2 . Here , we show that induction of EBV lytic program leads to degradation of p53 via an u...
Inhibition of p53-mediated transactivation is essential for regulating the cellular environment advantageous for viral infection . Specially , DNA viruses target p53 for inactivation through the ubiquitin-proteasome pathway . The E6 protein of the high-risk human papillomaviruses and the cellular ubiquitin-protein liga...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology/post-translational", "regulation", "of", "gene", "expression", "virology", "cell", "biology/cell", "signaling" ]
2009
Degradation of Phosphorylated p53 by Viral Protein-ECS E3 Ligase Complex
Intracellular pathogens include all viruses , many bacteria and parasites capable of invading and surviving within host cells . Key to survival is the subversion of host cell pathways by the pathogen for the purpose of propagation and evading the immune system . The intracellular bacterium Shigella flexneri , the causa...
Shigella flexneri is an intracellular bacterial pathogen and the causative agent of bacillary dysentery . It possesses the ability to invade and propagate within human cells by injecting bacterial effector proteins directly into host cells . Shortly after entry within a vacuole , S . flexneri induces vacuolar rupture a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "vesicles", "pathology", "and", "laboratory", "medicine", "vacuoles", "intracellular", "pathogens", "pathogens", "microbiology", "shigella", "molecular", "biology", "techniques", "cellular", "structures", "and", "organelles", "bact...
2016
Macropinosomes are Key Players in Early Shigella Invasion and Vacuolar Escape in Epithelial Cells
Japanese encephalitis ( JE ) is a flaviviral disease of public health concern in many parts of Asia . JE often occurs in large epidemics , has a high case-fatality ratio and , among survivors , frequently causes persistent neurological sequelae and mental disabilities . In 1997 , the Vietnamese government initiated imm...
Japanese encephalitis ( JE ) is a disease caused by a flavivirus transmitted by mosquitoes . Although pigs and wild birds are main reservoirs of the disease , it is occasionally transmitted to humans . The majority of infections in humans are asymptomatic . In persons developing encephalitis , JE has a high case-fatali...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "public", "health", "and", "epidemiology", "clinical", "research", "design", "infectious", "disease", "epidemiology", "flavivirus", "immunology", "vaccines", "neglected", "tropical", "diseases", "infectious", "disease", "control", "japanese", "encephalitis", "...
2012
Effectiveness of the Viet Nam Produced, Mouse Brain-Derived, Inactivated Japanese Encephalitis Vaccine in Northern Viet Nam
Transcriptome variation plays an important role in affecting the phenotype of an organism . However , an understanding of the underlying mechanisms regulating transcriptome variation in segregating populations is still largely unknown . We sought to assess and map variation in transcript abundance in maize shoot apices...
Phenotypes are determined by the expression of genes , the environment , and the interaction of gene expression and the environment . However , a complete understanding of the inheritance of and genome-wide regulation of gene expression is lacking . One approach , called expression quantitative trait locus ( eQTL ) map...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "biology", "population", "genetics", "crop", "genetics", "plant", "science", "cereals", "crops", "plant", "genomics", "population", "biology", "genetic", "polymorphism", "maize", "gene", "expression", "plant", "genetics", "biology", "agriculture", "plant", "b...
2013
Mendelian and Non-Mendelian Regulation of Gene Expression in Maize
The evolution of bacterial pathogenicity , heavily influenced by horizontal gene transfer , provides new virulence factors and regulatory connections that alter bacterial phenotypes . Salmonella pathogenicity islands 1 and 2 ( SPI-1 and SPI-2 ) are chromosomal regions that were acquired at different evolutionary times ...
Salmonella infect humans and a wide range of mammalian hosts . Successful infection requires the bacteria to sense their surroundings and regulate gene expression in a way that maximizes fitness in that particular environment . The two major lifestyles of Salmonella include extracellular stages and intracellular stages...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "immunology", "microbiology", "plasmid", "construction", "dna", "transcription", "bacterial", "diseases", "regu...
2017
The transcriptional regulator SsrB is involved in a molecular switch controlling virulence lifestyles of Salmonella
Autosomal dominant neovascular inflammatory vitreoretinopathy ( ADNIV ) is an autoimmune condition of the eye that sequentially mimics uveitis , retinitis pigmentosa , and proliferative diabetic retinopathy as it progresses to complete blindness . We identified two different missense mutations in the CAPN5 gene in thre...
We care for several families with an inherited form of autoimmune inflammation inside the eye . The patients also develop bleeding , scar tissue , and eventually blindness . Using advanced gene analysis methods , we discovered the cause of this disease is gene mutations in the CAPN5 gene . This gene makes a protein , c...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "ophthalmology", "genetics", "and", "genomics", "clinical", "genetics" ]
2012
Calpain-5 Mutations Cause Autoimmune Uveitis, Retinal Neovascularization, and Photoreceptor Degeneration
Dynamic regulation of leukocyte population size and activation state is crucial for an effective immune response . In malaria , Plasmodium parasites elicit robust host expansion of macrophages and monocytes , but the underlying mechanisms remain unclear . Here we show that myeloid expansion during P . chabaudi infectio...
Malaria , caused by Plasmodium parasites , places a huge disease burden on humankind . Efforts to develop an effective vaccine for this pathogen are hampered by a poor understanding of the kinds of immune responses needed for protection . When infected with Plasmodium , humans and other mammalian hosts develop greatly ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "immunology", "parasitic", "diseases", "parasitology", "animal", "models", "parasitemia", "bone", "marrow", "cells", "model", "organisms", "lymph", "nodes", "lymphatic", "system", "quantitat...
2016
Macrophage Colony Stimulating Factor Derived from CD4+ T Cells Contributes to Control of a Blood-Borne Infection
Endogenous RNAi ( endoRNAi ) is a conserved mechanism for fine-tuning gene expression . In the nematode Caenorhabditis elegans , several endoRNAi pathways are required for the successful development of reproductive cells . The CSR-1 endoRNAi pathway promotes germ cell development , primarily by facilitating the express...
During the oocyte-to-embryo transition , the control of development is transferred from the mother to the embryo . A key event during this transition is the transcriptional activation of the embryonic genome , which is tightly controlled . Here , by using the nematode C . elegans , we uncover a role for endogenous RNA ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "reproductive", "system", "rna", "interference", "gonads", "caenorhabditis", "messenger", "rna", "animals", "dna", "transcription", "germ", "cells", "animal", "models", "developmental", "biology", "oocytes", "cae...
2018
The CSR-1 endogenous RNAi pathway ensures accurate transcriptional reprogramming during the oocyte-to-embryo transition in Caenorhabditis elegans
Translation of hundreds of small ORFs ( smORFs ) of less than 100 amino acids has recently been revealed in vertebrates and Drosophila . Some of these peptides have essential and conserved cellular functions . In Drosophila , we have predicted a particular smORF class encoding ~80 aa hydrophobic peptides , which may fu...
In our genomes there are millions of short open reading frames that could produce small peptides of less than 100 amino-acids if translated . These sequences have been so far disregarded , but increasing evidence supports the notion that a subset of these–termed smORFs–are translated; however the function of most of th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "bacteriology", "invertebrates", "medicine", "and", "health", "sciences", "lysosomes", "immune", "cells", "vesicles", "immunology", "microbiology", "vertebrates", "animals", "animal", "models", "drosophila", "melanogaster", "model", "organisms", "bacteri...
2016
Hemotin, a Regulator of Phagocytosis Encoded by a Small ORF and Conserved across Metazoans
The human brain has the impressive capacity to adapt how it processes information to high-level goals . While it is known that these cognitive control skills are malleable and can be improved through training , the underlying plasticity mechanisms are not well understood . Here , we develop and evaluate a model of how ...
The human brain has the impressive ability to adapt how it processes information to high level goals . While it is known that these cognitive control skills are malleable and can be improved through training , the underlying plasticity mechanisms are not well understood . Here , we derive a computational model of how p...
[ "Abstract", "Introduction", "Models", "Results", "Discussion" ]
[ "learning", "control", "theory", "engineering", "and", "technology", "social", "sciences", "neuroscience", "learning", "and", "memory", "control", "engineering", "cognitive", "psychology", "systems", "science", "mathematics", "cognition", "vision", "computer", "and", "i...
2018
Rational metareasoning and the plasticity of cognitive control
Electrophysiological studies of the human heart face the fundamental challenge that experimental data can be acquired only from patients with underlying heart disease . Regarding human atria , there exist sizable gaps in the understanding of the functional role of cellular Ca2+ dynamics , which differ crucially from th...
In the human heart , the contraction of atrial and ventricular muscle cells is based largely on common mechanisms . There is , however , a fundamental difference in the cellular calcium dynamics that underlie the contractile function . Here , we have developed a computational model of the human atrial cell that convinc...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "cell", "biology/cell", "signaling", "cardiovascular", "disorders/arrhythmias,", "electrophysiology,", "and", "pacing", "physiology/cardiovascular", "physiology", "and", "circulation", "physiology/muscle", "and", "connective", "tissue", "computational", "biology/signaling", "netw...
2011
Impact of Sarcoplasmic Reticulum Calcium Release on Calcium Dynamics and Action Potential Morphology in Human Atrial Myocytes: A Computational Study
According to World Health Organization ( WHO ) prevalence estimates , 1 . 1 million people in Mexico are infected with Trypanosoma cruzi , the etiologic agent of Chagas disease ( CD ) . However , limited information is available about access to antitrypanosomal treatment . This study assesses the extent of access in Me...
Chagas disease is a vector-borne disease caused by the parasite Trypanosoma cruzi . The disease is most frequently transmitted by triatomine insects but can also be passed through blood donation or from mother to child at birth . Experts estimate that 8 million people are infected with Chagas disease globally and that ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2013
Barriers to Treatment Access for Chagas Disease in Mexico
Endothelial cells ( EC ) are the main target for Orientia tsutsugamushi infection and EC dysfunction is a hallmark of severe scrub typhus in patients . However , the molecular basis of EC dysfunction and its impact on infection outcome are poorly understood . We found that C57BL/6 mice that received a lethal dose of O ...
Scrub typhus is a life-threatening disease , caused by infection with O . tsutsugamushi , a Gram-negative bacterium that preferentially infects and replicates in the endothelium . Every year , approximately one million people are infected globally , especially in the Asia-Pacific region . However , the molecular mechan...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "death", "typhus", "medicine", "and", "health", "sciences", "innate", "immune", "system", "immune", "physiology", "cytokines", "pathology", "and", "laboratory", "medicine", "pathogens", "cell", "processes", "rna", "extraction", "microbiology", "immunology", "a...
2016
IL-33-Dependent Endothelial Activation Contributes to Apoptosis and Renal Injury in Orientia tsutsugamushi-Infected Mice
Trypanosoma cruzi is a protist parasite that causes Chagas disease . Several proteins that are essential for parasite virulence and involved in host immune responses are anchored to the membrane through glycosylphosphatidylinositol ( GPI ) molecules . In addition , T . cruzi GPI anchors have immunostimulatory activitie...
Chagas disease , considered one of the most neglected tropical diseases , is caused by the blood-borne parasite Trypanosoma cruzi and currently affects about 8 million people in Latin America . T . cruzi can be transmitted by insect vectors , blood transfusion , organ transplantation and mother-to-baby as well as throu...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "molecular", "cell", "biology", "microbiology", "biology", "genomics" ]
2013
Identification and Functional Analysis of Trypanosoma cruzi Genes That Encode Proteins of the Glycosylphosphatidylinositol Biosynthetic Pathway
Amebiasis , a global intestinal parasitic disease , is due to Entamoeba histolytica . This parasite , which feeds on bacteria in the large intestine of its human host , can trigger a strong inflammatory response upon invasion of the colonic mucosa . Whereas information about the mechanisms which are used by the parasit...
Entamoeba histolytica is a unicellular parasite which infects millions of humans worldwide via contaminated food and water . It resides in the colon and most infected individuals are asymptomatic . In some people , the parasite can spread into both intestinal and extraintestinal tissues , and results in amebiasis . E ....
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "trophozoites", "parasite", "groups", "medicine", "and", "health", "sciences", "oxidative", "stress", "caenorhabditis", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "animals", "parasitology", "animal", "models", "apicomplexa",...
2018
Escherichia coli mediated resistance of Entamoeba histolytica to oxidative stress is triggered by oxaloacetate
Perception is fundamentally underconstrained because different combinations of object properties can generate the same sensory information . To disambiguate sensory information into estimates of scene properties , our brains incorporate prior knowledge and additional “auxiliary” ( i . e . , not directly relevant to des...
To perceive your surroundings your brain must distinguish between different possible scenes , each of which is more or less likely . In order to disambiguate interpretations that are equally likely given sensory input , the brain aggregates multiple sensations to form an interpretation of the world consistent with each...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "neuroscience/sensory", "systems", "computational", "biology/computational", "neuroscience", "neuroscience/psychology", "neuroscience/experimental", "psychology", "neuroscience/natural", "and", "synthetic", "vision" ]
2010
Within- and Cross-Modal Distance Information Disambiguate Visual Size-Change Perception
Human T-lymphotropic virus type 1 ( HTLV-1 ) has worldwide distribution and is considered endemic in many world regions , including southwestern Japan and Brazil . Japanese immigrants and their descendants have a high risk of acquiring this infection due to intense population exchange between Brazil and Japan . This cr...
The population of Okinawan immigrants is considered vulnerable to human T-lymphotropic virus type 1 ( HTLV-1 ) infection because the Okinawa region in Japan is an endemic area . The second Brazilian largest Okinawan community is set in Campo Grande , Middle-West Brazil . This study aimed to estimate the prevalence and ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion", "Conclusion" ]
[]
2015
High Prevalence of HTLV-1 Infection among Japanese Immigrants in Non-endemic Area of Brazil