mahendrawada_2025 / README.md
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Removed applies_to for wt_degron_control_counts
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metadata
license: mit
language:
  - en
tags:
  - biology
  - genomics
  - yeast
  - transcription-factors
  - gene-expression
  - binding
  - chec
  - perturbation
  - rnaseq
  - nascent rnaseq
pretty_name: Mahendrawada 2025 ChEC-seq and Nascent RNA-seq data
size_categories:
  - 100K<n<1M
experimental_conditions:
  temperature_celsius: 30
  growth_phase_at_harvest:
    od600: 1
  media:
    name: synthetic_complete
    nitrogen_source:
      - compound: yeast_nitrogen_base
        concentration_percent: 0.17
        specifications:
          - without_ammonium_sulfate
          - without_amino_acids
      - compound: ammonium_sulfate
        concentration_percent: 0.5
      - compound: amino_acid_dropout_mix
        concentration_percent: 0.06
      - compound: adenine_sulfate
        concentration_percent: 0.004
      - compound: uracil
        concentration_percent: 0.0002
configs:
  - config_name: genomic_features
    description: >-
      Comprehensive genomic features and regulatory characteristics for yeast
      genes
    dataset_type: genomic_features
    data_files:
      - split: train
        path: features_mahendrawada_2025.parquet
    dataset_info:
      features:
        - name: gene_id
          dtype: string
          description: >-
            Systematic gene name (ORF identifier) from SGD
            (https://yeastgenome.org/)
        - name: SGD_id
          dtype: string
          description: Unique identifier for each gene from SGD (https://yeastgenome.org/)
        - name: gene_name
          dtype: string
          description: Common name of each gene
        - name: chr
          dtype: string
          description: Chromosome number corresponding to gene
        - name: strand
          dtype: string
          description: Strandedness of the gene (+ or -)
        - name: start
          dtype: int64
          description: Start position of the ORF
        - name: end
          dtype: int64
          description: End position of the ORF
        - name: TSS
          dtype: int64
          description: >-
            Transcription start site based on Park et al., 2014
            (doi:10.1093/nar/gkt1366)
        - name: TATA_category
          dtype: string
          description: >-
            TATA box classification from Donczew et al., 2020 using consensus
            TATAWAW (doi:10.7554/eLife.50109)
        - name: expression
          dtype: float64
          description: >-
            Average signal normalized to gene length from Donczew et al., 2020
            (doi:10.7554/eLife.50109)
        - name: +1 nucleosome
          dtype: float64
          description: >-
            Position of +1 nucleosome from Chereji et al., 2018
            (doi:10.1186/S13059-018-1398-0)
        - name: '-1 nucleosome'
          dtype: float64
          description: >-
            Position of -1 nucleosome from Chereji et al., 2018
            (doi:10.1186/S13059-018-1398-0)
        - name: NDR Center
          dtype: float64
          description: >-
            Center of nucleosome depleted region from Chereji et al., 2018
            (doi:10.1186/S13059-018-1398-0)
        - name: NDR Width
          dtype: float64
          description: >-
            Width of nucleosome depletion region from Chereji et al., 2018
            (doi:10.1186/S13059-018-1398-0)
        - name: tail-dependence
          dtype: string
          description: >-
            Tail classification based on Mediator tail dependence from Warfield
            L, Donczew R et al., 2022 (doi:10.1016/j.molcel.2022.09.016)
        - name: coactivator
          dtype: string
          description: >-
            Coactivator classification based on TFIID and/or SAGA dependence
            from Donczew et al., 2020 (doi:10.7554/eLife.50109)
        - name: LCID_center
          dtype: string
          description: >-
            Genes near boundaries of chromosomal interacting domains from
            Swygert et al., 2020 (doi:10.1016/j.molcel.2018.11.020)
        - name: Rossi_classes
          dtype: string
          description: >-
            Promoter classes from Rossi et al., 2021
            (doi:10.1038/s41586-021-03314-8)
        - name: RP_category
          dtype: string
          description: >-
            Ribosomal protein (RP) and ribosomal biogenesis (RiBi) gene
            classification from Zencir et al., 2020 (doi:10.1093/NAR/GKAA852)
        - name: binding_cluster
          dtype: string
          description: >-
            Clusters from unsupervised K-means clustering using binary binding
            data of 178 transcription factors
        - name: list_of_TFS_bound
          dtype: string
          description: >-
            List of transcription factors bound to gene promoter (-400 to +200
            bp from TSS; Homer peak calling)
        - name: number_of_bound_tfs
          dtype: int64
          description: Number of transcription factors bound to each promoter
        - name: locus_tag
          dtype: string
          description: Systematic gene identifier from yeast_genome_resources dataset
        - name: symbol
          dtype: string
          description: Standard gene symbol from yeast_genome_resources dataset
  - config_name: mahendrawada_chec_seq
    description: >-
      ChEC-seq transcription factor binding data with peak scores (original
      authors' processed data)
    default: true
    dataset_type: annotated_features
    metadata_fields:
      - regulator_locus_tag
      - regulator_symbol
    data_files:
      - split: train
        path: chec_mahendrawada_2025.parquet
    dataset_info:
      features:
        - name: sample_id
          dtype: integer
          description: >-
            unique identifier for a specific sample, which uniquely identifies
            one of the 178 TFs. Across datasets in this repo, the a given
            sample_id identifies the same regulator.
        - name: regulator_locus_tag
          dtype: string
          description: Systematic gene name (ORF identifier) of the transcription factor
        - name: regulator_symbol
          dtype: string
          description: Standard gene symbol of the transcription factor
        - name: target_locus_tag
          dtype: string
          description: Systematic gene name (ORF identifier) of the target gene
        - name: target_symbol
          dtype: string
          description: Standard gene symbol of the target gene
        - name: peak_score
          dtype: float64
          description: >-
            ChEC signal around peak center (sum of ChEC signal from -150 to +150
            bp from peak summit) normalized to Drosophila spike-in control
        - name: processing_method
          dtype: string
          description: Method used for peak calling and quantification (original authors)
  - config_name: chec_genome_map_meta
    description: >-
      Sample-level metadata for ChEC-seq experiments including regulator
      information, experimental conditions, and replicate structure
    dataset_type: metadata
    applies_to:
      - chec_mahendrawada_m2025_af_replicates
      - chec_genome_map
    data_files:
      - split: train
        path: chec_genome_map_meta.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: >-
            SRA (Sequence Read Archive) accession identifier for this biological
            replicate
          role: sample_id
        - name: mahendrawada_symbol
          dtype: string
          description: Transcription factor symbol as reported in Mahendrawada et al. 2025
        - name: replicate
          dtype: string
          description: Biological replicate identifier. One of A,B,C. All have 3 replicates
        - name: condition
          dtype:
            class_label:
              names:
                - standard
                - SM
                - WT
                - WT_SM
                - admut
                - admut_SM
                - cAD
                - cAD_SM
                - dbdmut
                - dbdmut_SM
                - nAD
                - nAD_SM
                - ncAD
                - ncAD_SM
                - '30'
                - '37'
                - galactose
                - raffinose
          description: Experimental condition for this sample
          role: experimental_condition
        - name: regulator_locus_tag
          dtype: string
          description: >-
            Systematic gene name (ORF identifier) of the depleted transcription
            factor
          role: regulator_identifier
        - name: regulator_symbol
          dtype: string
          description: Standard gene symbol of the depleted transcription factor
          role: regulator_identifier
        - name: sample_id
          dtype: int
          description: >-
            Unique identifier combining regulator, condition, and replicates
            (used in combined datasets)
  - config_name: chec_genome_map
    description: >-
      ChEC-seq data reprocessed in the Brent Lab. See scripts/ for more details.
      These are bed format, therefore intervals are 0-based, half open, eg
      chrI:0-10 in a bed format would be chr:1-10 in IGV which is 1-indexed,
      closed interval
    dataset_type: genome_map
    data_files:
      - split: train
        path: chec_genome_map/sra_accession=*/*.parquet
    dataset_info:
      partitioning:
        enabled: true
        partition_by:
          - sra_accession
      features:
        - name: sra_accession
          dtype: string
          description: >-
            SRA accession identifier used for partitioning (links to
            chec_genome_map_meta)
          role: sample_id
        - name: chr
          dtype: string
          description: Chromosome identifier (e.g., chrI, chrII, chrXVI)
        - name: start
          dtype: int
          description: Feature start position (0-based half open)
        - name: end
          dtype: int
          description: Feature end position (0-based, half open)
        - name: name
          dtype: string
          description: >-
            This stores the character `.`, one of the accepted characters for NA
            in bed6
        - name: score
          dtype: int
          description: The depth of 5 prime read ends at this location
          role: quantitative_measure
        - name: strand
          dtype: string
          description: >-
            Strand orientation of the mapped fragment from which the 5 prime end
            originates (+ or -)
  - config_name: chec_genome_map_control_meta
    description: Sample-level metadata for ChEC free MNase experiments
    dataset_type: metadata
    applies_to:
      - chec_genome_map_control
    data_files:
      - split: train
        path: chec_genome_map_control_meta.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: >-
            SRA (Sequence Read Archive) accession identifier for this biological
            replicate
        - name: replicate
          dtype: string
          description: Biological replicate identifier. One of A,B,C. All have 3 replicates
        - name: notes
          dtype: string
          description: Additional notes or experimental details for this sample
  - config_name: chec_genome_map_control
    description: >-
      ChEC free MNase genome-wide signal tracks partitioned by SRA accession,
      providing position-level chromatin accessibility data
    dataset_type: genome_map
    data_files:
      - split: train
        path: chec_genome_map_control/sra_accession=*/*.parquet
    dataset_info:
      partitioning:
        enabled: true
        partition_by:
          - sra_accession
      features:
        - name: sra_accession
          dtype: string
          description: >-
            SRA accession identifier used for partitioning (links to
            chec_genome_map_control_meta)
          role: sample_id
        - name: chr
          dtype: string
          description: Chromosome identifier (e.g., chrI, chrII, chrXVI)
        - name: start
          dtype: int
          description: Feature start position (0-based half open)
        - name: end
          dtype: int
          description: Feature end position (0-based, half open)
        - name: name
          dtype: string
          description: >-
            This stores the character `.`, one of the accepted characters for NA
            in bed6
        - name: score
          dtype: int
          description: The depth of 5 prime read ends at this location
          role: quantitative_measure
        - name: strand
          dtype: string
          description: >-
            Strand orientation of the mapped fragment from which the 5 prime end
            originates (+ or -)
  - config_name: chec_mahendrawada_m2025_af_replicates
    description: >-
      ChEC-seq annotated features at biological replicate level with binding
      peaks and statistical significance metrics
    dataset_type: annotated_features
    data_files:
      - split: train
        path: chec_mahendrawada_m2025_af_replicates.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: >-
            SRA (Sequence Read Archive) accession identifier for this biological
            replicate
          role: sample_id
        - name: target_locus_tag
          dtype: string
          description: Systematic gene identifier for the target gene
          role: target_identifier
        - name: target_symbol
          dtype: string
          description: Standard gene symbol for the target gene
          role: target_identifier
        - name: seqnames
          dtype: string
          description: Chromosome identifier (e.g., chrI, chrII)
        - name: start
          dtype: int64
          description: Peak start position (0-based coordinate)
        - name: end
          dtype: int64
          description: Peak end position (0-based, exclusive)
        - name: width
          dtype: int64
          description: Width of the peak in base pairs
        - name: strand
          dtype: string
          description: Strand orientation (+ or -)
        - name: enrichment
          dtype: float64
          description: Enrichment score for the binding peak
          role: quantitative_measure
        - name: poisson_pval
          dtype: float64
          description: P-value from Poisson distribution test for peak significance
          role: quantitative_measure
        - name: log_poisson_pval
          dtype: float64
          description: Log-transformed Poisson p-value
          role: quantitative_measure
        - name: hypergeometric_pval
          dtype: float64
          description: P-value from hypergeometric distribution test for peak significance
          role: quantitative_measure
        - name: log_hypergeometric_pval
          dtype: float64
          description: Log-transformed hypergeometric p-value
          role: quantitative_measure
        - name: poisson_qval
          dtype: float64
          description: FDR-adjusted q-value from Poisson test (multiple testing correction)
          role: quantitative_measure
        - name: hypergeometric_qval
          dtype: float64
          description: >-
            FDR-adjusted q-value from hypergeometric test (multiple testing
            correction)
          role: quantitative_measure
  - config_name: chec_mahendrawada_m2025_af_combined_meta
    description: >-
      Sample-level metadata for combined ChEC-seq experiments with regulator
      information and experimental conditions
    dataset_type: metadata
    applies_to:
      - chec_mahendrawada_m2025_af_combined
    data_files:
      - split: train
        path: chec_mahendrawada_m2025_af_combined_meta.parquet
    dataset_info:
      features:
        - name: sample_id
          dtype: string
          description: Unique identifier combining regulator, condition, and replicates
        - name: regulator_locus_tag
          dtype: string
          description: >-
            Systematic gene name (ORF identifier) of the depleted transcription
            factor
          role: regulator_identifier
        - name: regulator_symbol
          dtype: string
          description: Standard gene symbol of the depleted transcription factor
          role: regulator_identifier
        - name: mahendrawada_symbol
          dtype: string
          description: Transcription factor symbol as reported in Mahendrawada et al. 2025
        - name: condition
          dtype:
            class_label:
              names:
                - standard
                - SM
                - WT
                - WT_SM
                - admut
                - admut_SM
                - cAD
                - cAD_SM
                - dbdmut
                - dbdmut_SM
                - nAD
                - nAD_SM
                - ncAD
                - ncAD_SM
                - '30'
                - '37'
                - galactose
                - raffinose
          description: Experimental condition for this sample
          role: experimental_condition
  - config_name: chec_mahendrawada_m2025_af_combined
    description: >-
      Brent Lab reprocessed data from Mahendrawada 2025. See scripts/ for more
      details.
    dataset_type: annotated_features
    data_files:
      - split: train
        path: chec_mahendrawada_m2025_af_combined.parquet
    dataset_info:
      features:
        - name: sample_id
          dtype: string
          description: >-
            Unique identifier for a specific regulator/condition. These are made
            up of the replicates marked as `combine == TRUE` in the replicate
            level metadata
        - name: target_locus_tag
          dtype: string
          description: Systematic gene identifier for the target gene
          role: target_identifier
        - name: target_symbol
          dtype: string
          description: Standard gene symbol for the target gene
          role: target_identifier
        - name: seqnames
          dtype: string
          description: Chromosome identifier (e.g., chrI, chrII)
        - name: start
          dtype: int64
          description: Peak start position (1-based)
        - name: end
          dtype: int64
          description: Peak end position (1-based, inclusive)
        - name: width
          dtype: int64
          description: Width of the peak in base pairs
        - name: strand
          dtype: string
          description: Strand orientation (+ or -)
        - name: enrichment
          dtype: float64
          description: Enrichment of experiment vs background
          role: quantitative_measure
        - name: poisson_pval
          dtype: float64
          description: P-value from Poisson distribution test for peak significance
          role: quantitative_measure
        - name: log_poisson_pval
          dtype: float64
          description: Log-transformed Poisson p-value
          role: quantitative_measure
        - name: hypergeometric_pval
          dtype: float64
          description: P-value from hypergeometric distribution test for peak significance
          role: quantitative_measure
        - name: log_hypergeometric_pval
          dtype: float64
          description: Log-transformed hypergeometric p-value
          role: quantitative_measure
        - name: poisson_qval
          dtype: float64
          description: FDR-adjusted q-value from Poisson test (multiple testing correction)
          role: quantitative_measure
        - name: hypergeometric_qval
          dtype: float64
          description: >-
            FDR-adjusted q-value from hypergeometric test (multiple testing
            correction)
          role: quantitative_measure
  - config_name: rna_seq
    description: >-
      Nascent RNA-seq differential expression data following transcription
      factor depletion using 4TU metabolic labeling
    dataset_type: annotated_features
    metadata_fields:
      - regulator_locus_tag
      - regulator_symbol
    data_files:
      - split: train
        path: rnaseq_mahendrawada_2025.parquet
    dataset_info:
      features:
        - name: sample_id
          dtype: integer
          description: >-
            unique identifier for a specific sample, which uniquely identifies
            one of the 178 TFs. Across datasets in this repo, the a given
            sample_id identifies the same regulator.
        - name: db_id
          dtype: integer
          description: >-
            an old unique identifer, for use internally only. Deprecated and
            will be removed eventually. Do not use in analysis.
        - name: regulator_locus_tag
          dtype: string
          description: >-
            Systematic gene name (ORF identifier) of the depleted transcription
            factor
        - name: regulator_symbol
          dtype: string
          description: Standard gene symbol of the depleted transcription factor
        - name: target_locus_tag
          dtype: string
          description: >-
            Systematic gene name (ORF identifier) of the differentially
            expressed target gene
        - name: target_symbol
          dtype: string
          description: Standard gene symbol of the differentially expressed target gene
        - name: log2fc
          dtype: float64
          description: >-
            Log2 fold change (IAA/DMSO) for significantly affected genes
            (DESeq2, padj <0.1, FC >= 1.3)
  - config_name: rnaseq_reprocessed
    description: >-
      Reprocessed nascent RNA-seq differential expression data using DESeq2
      without thresholding, comparing IAA-induced TF degradation versus DMSO
      control
    dataset_type: annotated_features
    metadata_fields:
      - regulator_locus_tag
      - regulator_symbol
      - sample_id
      - env_condition
      - timepoint
    data_files:
      - split: train
        path: rnaseq_reprocessed.parquet
    dataset_info:
      features:
        - name: sample_id
          dtype: string
          description: >-
            Composite identifier combining regulator, condition, timepoint, and
            treatment information from the merged IAA and DMSO sample IDs
          role: sample_id
        - name: regulator_locus_tag
          dtype: string
          description: Systematic gene identifier for the depleted transcription factor
          role: regulator_identifier
        - name: regulator_symbol
          dtype: string
          description: Standard gene symbol for the depleted transcription factor
          role: regulator_identifier
        - name: env_condition
          dtype:
            class_label:
              names:
                - standard_30C
                - SM
                - galactose
                - raffinose
                - heat_shock_37C
          description: Environmental growth condition for this experiment
          role: experimental_condition
        - name: timepoint
          dtype: float64
          description: >-
            Time point in minutes (standard is 30 minutes post-treatment. very
            few other timepoints)
          role: experimental_condition
        - name: target_locus_tag
          dtype: string
          description: >-
            Systematic gene identifier for the differentially expressed target
            gene
          role: target_identifier
        - name: target_symbol
          dtype: string
          description: Standard gene symbol for the differentially expressed target gene
          role: target_identifier
        - name: baseMean
          dtype: float64
          description: Mean of normalized counts across all samples (DESeq2 output)
          role: quantitative_measure
        - name: log2FoldChange
          dtype: float64
          description: >-
            Log2 fold change IAA versus DMSO (DESeq2 output, no thresholding
            applied)
          role: quantitative_measure
        - name: lfcSE
          dtype: float64
          description: Standard error of the log2 fold change estimate (DESeq2 output)
          role: quantitative_measure
        - name: stat
          dtype: float64
          description: Wald test statistic (DESeq2 output)
          role: quantitative_measure
        - name: pvalue
          dtype: float64
          description: Wald test p-value (DESeq2 output)
          role: quantitative_measure
        - name: padj
          dtype: float64
          description: Benjamini-Hochberg adjusted p-value (DESeq2 output)
          role: quantitative_measure
  - config_name: degron_counts_meta
    description: >-
      Sample-level metadata for auxin-inducible degron perturbation experiments
      with HTSeq count statistics
    dataset_type: metadata
    applies_to:
      - degron_counts
    data_files:
      - split: train
        path: degron_counts_meta.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: SRA run accession identifier for this sample
          role: sample_identifier
        - name: gsm_accession
          dtype: string
          description: GEO sample accession identifier (GSM ID)
        - name: regulator_locus_tag
          dtype: string
          description: Systematic gene identifier for the transcription factor
          role: regulator_identifier
        - name: regulator_symbol
          dtype: string
          description: Standard gene symbol for the transcription factor
          role: regulator_identifier
        - name: degron_treatment
          dtype:
            class_label:
              names:
                - DMSO
                - IAA
          description: Degron treatment condition (DMSO control or IAA degradation)
          role: experimental_condition
        - name: degron_variant
          dtype:
            class_label:
              names:
                - mini_N_terminal_IAA7
                - full_or_short_IAA7
          description: Type of auxin-inducible degron tag used
        - name: env_condition
          dtype:
            class_label:
              names:
                - standard_30C
                - SM
                - galactose
                - raffinose
                - heat_shock_37C
          description: Environmental growth condition
          role: experimental_condition
        - name: timepoint
          dtype: float64
          description: Time point for time-series experiments (minutes)
        - name: replicate
          dtype: string
          description: Biological replicate identifier (A, B, or C)
        - name: no_feature
          dtype: int64
          description: HTSeq count of reads not assigned to any feature
        - name: ambiguous
          dtype: int64
          description: HTSeq count of reads mapping ambiguously to multiple features
        - name: too_low_aQual
          dtype: int64
          description: HTSeq count of reads filtered due to low alignment quality
        - name: alignment_not_unique
          dtype: int64
          description: HTSeq count of reads with non-unique alignments
        - name: sample_id
          dtype: string
          description: Unique sample identifier combining replicates by treatment
  - config_name: degron_counts
    description: >-
      HTSeq gene-level read counts for auxin-inducible degron perturbation
      experiments
    dataset_type: annotated_features
    data_files:
      - split: train
        path: degron_counts.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: SRA run accession identifier linking to sample metadata
          role: sample_id
        - name: target_locus_tag
          dtype: string
          description: >-
            Systematic gene identifier from BrentLab yeast genomic features
            (compatible with BrentLab yeast collection)
          role: target_identifier
        - name: target_symbol
          dtype: string
          description: >-
            Standard gene symbol from BrentLab yeast genomic features
            (compatible with BrentLab yeast collection)
          role: target_identifier
        - name: orig_locus_tag
          dtype: string
          description: >-
            Original systematic gene identifier from R64-1-1 genome annotation
            as reported by Mahendrawada et al. 2025
        - name: count
          dtype: int64
          description: HTSeq gene-level read count from R64-1-1 genome annotation
          role: quantitative_measure
  - config_name: mnase_fusion_rnaseq_counts_meta
    description: >-
      Sample-level metadata for MNase fusion strain RNA-seq experiments with
      HTSeq count statistics
    dataset_type: metadata
    applies_to:
      - mnase_fusion_rnaseq_counts
    data_files:
      - split: train
        path: mnase_fusion_rnaseq_counts_meta.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: SRA run accession identifier for this sample
          role: sample_identifier
        - name: gsm_accession
          dtype: string
          description: GEO sample accession identifier (GSM ID)
        - name: regulator_locus_tag
          dtype: string
          description: Systematic gene identifier for the MNase-tagged transcription factor
          role: regulator_identifier
        - name: regulator_symbol
          dtype: string
          description: Standard gene symbol for the MNase-tagged transcription factor
          role: regulator_identifier
        - name: env_condition
          dtype:
            class_label:
              names:
                - standard_30C
                - SM
                - galactose
                - raffinose
                - heat_shock_37C
          description: Environmental growth condition
          role: experimental_condition
        - name: replicate
          dtype: string
          description: Biological replicate identifier (A, B, or C)
        - name: no_feature
          dtype: int64
          description: HTSeq count of reads not assigned to any feature
        - name: ambiguous
          dtype: int64
          description: HTSeq count of reads mapping ambiguously to multiple features
        - name: too_low_aQual
          dtype: int64
          description: HTSeq count of reads filtered due to low alignment quality
        - name: alignment_not_unique
          dtype: int64
          description: HTSeq count of reads with non-unique alignments
        - name: sample_id
          dtype: string
          description: Unique sample identifier combining replicates by treatment
  - config_name: mnase_fusion_rnaseq_counts
    description: HTSeq gene-level read counts for MNase fusion strain RNA-seq experiments
    dataset_type: annotated_features
    data_files:
      - split: train
        path: mnase_fusion_rnaseq_counts.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: SRA run accession identifier linking to sample metadata
          role: sample_id
        - name: target_locus_tag
          dtype: string
          description: >-
            Systematic gene identifier from BrentLab yeast genomic features
            (compatible with BrentLab yeast collection)
          role: target_identifier
        - name: target_symbol
          dtype: string
          description: >-
            Standard gene symbol from BrentLab yeast genomic features
            (compatible with BrentLab yeast collection)
          role: target_identifier
        - name: orig_locus_tag
          dtype: string
          description: >-
            Original systematic gene identifier from R64-1-1 genome annotation
            as reported by Mahendrawada et al. 2025
        - name: count
          dtype: int64
          description: HTSeq gene-level read count from R64-1-1 genome annotation
          role: quantitative_measure
  - config_name: wt_baseline_counts_meta
    description: >-
      Sample-level metadata for wild-type baseline RNA-seq experiments with
      HTSeq count statistics
    dataset_type: metadata
    applies_to:
      - wt_baseline_counts
    data_files:
      - split: train
        path: wt_baseline_counts_meta.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: SRA run accession identifier for this sample
          role: sample_identifier
        - name: gsm_accession
          dtype: string
          description: GEO sample accession identifier (GSM ID)
        - name: env_condition
          dtype:
            class_label:
              names:
                - standard_30C
                - SM
                - galactose
                - raffinose
                - heat_shock_37C
          description: Environmental growth condition
          role: experimental_condition
        - name: replicate
          dtype: string
          description: Biological replicate identifier (A, B, or C)
        - name: no_feature
          dtype: int64
          description: HTSeq count of reads not assigned to any feature
        - name: ambiguous
          dtype: int64
          description: HTSeq count of reads mapping ambiguously to multiple features
        - name: too_low_aQual
          dtype: int64
          description: HTSeq count of reads filtered due to low alignment quality
        - name: alignment_not_unique
          dtype: int64
          description: HTSeq count of reads with non-unique alignments
        - name: sample_id
          dtype: string
          description: Unique sample identifier combining replicates by treatment
  - config_name: wt_baseline_counts
    description: HTSeq gene-level read counts for wild-type baseline RNA-seq experiments
    dataset_type: annotated_features
    data_files:
      - split: train
        path: wt_baseline_counts.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: SRA run accession identifier linking to sample metadata
          role: sample_id
        - name: target_locus_tag
          dtype: string
          description: >-
            Systematic gene identifier from BrentLab yeast genomic features
            (compatible with BrentLab yeast collection)
          role: target_identifier
        - name: target_symbol
          dtype: string
          description: >-
            Standard gene symbol from BrentLab yeast genomic features
            (compatible with BrentLab yeast collection)
          role: target_identifier
        - name: orig_locus_tag
          dtype: string
          description: >-
            Original systematic gene identifier from R64-1-1 genome annotation
            as reported by Mahendrawada et al. 2025
        - name: count
          dtype: int64
          description: HTSeq gene-level read count from R64-1-1 genome annotation
          role: quantitative_measure
  - config_name: wt_degron_control_counts_meta
    description: >-
      Sample-level metadata for wild-type degron control RNA-seq experiments
      with HTSeq count statistics
    dataset_type: metadata
    applies_to:
      - wt_degron_control_counts
    data_files:
      - split: train
        path: wt_degron_control_counts_meta.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: SRA run accession identifier for this sample
          role: sample_identifier
        - name: gsm_accession
          dtype: string
          description: GEO sample accession identifier (GSM ID)
        - name: degron_treatment
          dtype:
            class_label:
              names:
                - DMSO
                - IAA
          description: Degron treatment condition (DMSO control or IAA degradation)
          role: experimental_condition
        - name: replicate
          dtype: string
          description: Biological replicate identifier (A, B, or C)
        - name: no_feature
          dtype: int64
          description: HTSeq count of reads not assigned to any feature
        - name: ambiguous
          dtype: int64
          description: HTSeq count of reads mapping ambiguously to multiple features
        - name: too_low_aQual
          dtype: int64
          description: HTSeq count of reads filtered due to low alignment quality
        - name: alignment_not_unique
          dtype: int64
          description: HTSeq count of reads with non-unique alignments
        - name: sample_id
          dtype: string
          description: Unique sample identifier combining replicates by treatment
  - config_name: wt_degron_control_counts
    description: >-
      HTSeq gene-level read counts for wild-type degron control RNA-seq
      experiments
    dataset_type: annotated_features
    data_files:
      - split: train
        path: wt_degron_control_counts.parquet
    dataset_info:
      features:
        - name: sra_accession
          dtype: string
          description: SRA run accession identifier linking to sample metadata
          role: sample_id
        - name: target_locus_tag
          dtype: string
          description: >-
            Systematic gene identifier from BrentLab yeast genomic features
            (compatible with BrentLab yeast collection)
          role: target_identifier
        - name: target_symbol
          dtype: string
          description: >-
            Standard gene symbol from BrentLab yeast genomic features
            (compatible with BrentLab yeast collection)
          role: target_identifier
        - name: orig_locus_tag
          dtype: string
          description: >-
            Original systematic gene identifier from R64-1-1 genome annotation
            as reported by Mahendrawada et al. 2025
        - name: count
          dtype: int64
          description: HTSeq gene-level read count from R64-1-1 genome annotation
          role: quantitative_measure

Mahendrawada 2025

This data is taken from the Supplement of

Mahendrawada, L., Warfield, L., Donczew, R. et al. Low overlap of transcription factor DNA binding and regulatory targets. Nature 642, 796–804 (2025). https://doi.org/10.1038/s41586-025-08916-0

and GSE236948