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Stored Procedure is one of my favorite database objects in Microsoft SQL Server. In Data Processing Continuum, SP is more near to Data then view or batch. Moving nearer to data for good performance and data integrity is a rule of thumb in SQL Server. Creating your logic in Stored Procedure is not only improving performance and integrity but it reduce the network traffic as well because you are not making logic in client application but making it in SQL Server itself.
Anyway, let us now focus on our core topic of the article.
–SP are compiled object so it is anyway fastest than batch or T-SQL query.
–Provide consistency as you can use one stored procedure at many places in client application and at the time of modification all you need to do is, change at one place and you will done.
–provide abstraction layer and security as it hides the table name.
Why is it boosting up the speed?
Answer is quite simple as I described earlier is, it used to compile the Stored Procedure at first run, creates query plan for that and store it in memory so than repeated use of same stored procedure can be freed out from the burden of creating query plan. You can see stored query plan with following query in SSMS.
Note: below query will show you all compiled plan stored in system, not only those who are stored via stored procedures only.
Be aware that, all the plans for stored procedure will not stay forever in syscacheobjects. It will obsolete when any index, on the table we have used in SP, is created/ dropped or big data manipulation performed on the table. However, you can mark your stored procedure as recompiled by following command in SSMS.
There are mainly three commands to manage stored procedure. ALTER, CREATE and DROP.
–after creating SP,let us check whether it actually works!!!
I will provide you with more examples with complex structure of stored procedure very soon. | 2019-04-23T10:21:48Z | https://riteshshah.wordpress.com/category/drop-proc/ |
Vienna, Austria, August 1, 2018 – Things can change quickly in beach volleyball and the Grimalt cousins Marco and Esteban are the most recent example of it. Just two days after winning the first ever FIVB World Tour gold medal for their country in Tokyo, the Chileans are competing in the qualification tournament of the $600,000 A1 Major Vienna presented by Swatch.
The Rio 2016 Olympians were one of the 16 men’s teams to win their opening match in the Austrian tournament as they posted a 2-0 (23-21, 21-16) victory over Sweden’s Martin Appelgren and Simon Bohman.
They will play Switzerland’s Marco Krattiger and Nico Beeler in the next round for a spot in the main draw.
If their physical conditions might have been negatively affected by the demanding tournament they played in Japan, their morale was certainly boosted by the unprecedented gold medal. And they want to keep the momentum going on the Danube Island as the Rio 2016 Olympians are looking forward to their best finish in a Major event in 2018 after finishing 25th in Fort Lauderdale and 33rd in Gstaad. | 2019-04-25T16:49:11Z | http://worldtour.2018.fivb.com/en/news/after-historical-gold-the-grimalts-play-for?id=77252 |
I run a 1972 Chevelle in a Pure Stock class.
I replaced the right front spring with a spring from a big block Chevelle. The car is still way to soft. Anyone have any suggestions on how to stiffen this car up and still be legal (no racing springs). Is their a truck spring or some other vehicle with stiffer springs that I can use. Chevelle springs are 5 1/2" dia. by 12" long.I had spring rubbers in but I had to remove them per the rules.
Are you using the sway bar on front?
Our rules read the same in the pure stock class.We got the "stock appearing" springs suppose to be same diameter as stock.A oldtimmer says BLACK is stock so I painted them black,rolled them around in the dirt,sand and gravel set them in the sun and put them up under the car.I overheard another driver and a tech talking about removing a spring for inspection.....the driver says it would put him and others in danger to attemp removing spring at the track without the proper equipment.The tech agreed and nothing eles was ever said about it.I think I will use the same story if they asked me to pull a spring out of our pure stock at the track.Another thing I learned from an oldtimmer was NEVER lend a tech your flashlight to tech your car.....yet ALWAYS offer your flashlight to the tech to tech someone eles car!!!!
carfule double revenge can be a ***** sometimes.
his rules say no racing springs!!!! why cheat? if you wanna use racing springs just move up to the next class, the lower classes are for lower budget guys, why ruin it for them? sleepy21 look around the junkyards and youll find a stock spring that will work for you somehow, and then when you pass those guys that are cheating you will have a bigger smile on your face than if you were cheating!! GOOD LUCK!!
I use a front spring out of a big block 1970 pontiac catalina. Then i heated it up and shrunk a coil down to stiffen it up. Works perfect. I do have the sway bar on too though.
Hi there well dont heat the spring it does not help spring rate one thing you can do is look for a stock longer spring and cut it that will increase rate alot depending but heating doesnt creat more spring rate just lowers car and helps to have a spring bind later. We run a ford torino and they are the same diameter that you are looking for so you might try that. Cut them to the heighth you want and then go check them at your local speed shop.
I have used alot of different springs to do what you are doing. big Cadillac springs, Lincoln Continental, F100 trucks, whatever we found at the you pull it yard that met our measurement requirements as far as diameter and thickness, then just cut them to what we needed to get ride height and called it good. That was 20 years ago but would still be true today. Any parts stores that can read a catalog also has a listing in the back the gives spring rates and dimensions. At least Moog does you just gotta find someone that can read it. But if you find what you are after, then hit the local yard and search for that vehicle. I guess it was easier for me then, I was working at the parts store but there are a few good ones left that can do that for you.
Mr. Dupree, that's not entirely true. If you heat a spring to the point where one coil collapses on another, it would be equivalent to cutting a coil. The 3 things that determine spring rate are the wire diameter, the diameter of the coil, and the number of active coils. I know this isn't the preferred method by the majority so cutting is probably still the best option.
Well Subwitchew I just wanted to give him the best way to achieve what he wanted and do it right. We use to heat are springs but very hard to get excactly what height you want or know just how much you increased the spring preasure. But i guess thats up to want you want Goodluck and i am sure he has enough info now to get the springs he needs.
I read in one of the Circle Track articles a couple of years ago that if you cut the spring it increases the spring rate. They suggested cutting a 1/2 coil at a time. I just can't remember how much the spring rate increased. They had it figured out I just don't remember. They also said that heating the springs was a bad idea because it just weakens the spring and makes it more prone to failure. I've seen springs crack and break where they were heated up. I'm sure the guy who did it got it to hot but how do you really know for sure?
Mr. Dupree, you did give him the best way (outside of buying new springs. ). I meant nothing by my statement. I agree with you that cutting is much better than heating. The only reason I even said anything was because people on here rely on this information. It should be as accurate as possible. If you heat a spring and collapse it, it will increase the spring rate.
crc1124- Spring rate is directly proportional to the number of active coils (as long as it is not a progressive spring). If you reduce the number of active coils by half, you will double the spring rate. So if there were 5 active coils and you cut off half a coil, you would increase spring rate by 10%. Hope this helps.
We totally understand we love the help we get here from the dirt forum. Sorry i should have explained my self more in my statement. Talk at you soon fellow drivers and tahnks for all the help too.
I replaced the right front spring with a spring from a big block Chevelle. The car is still way to soft. Anyone have any suggestions on how to stiffen this car up and still be legal (no racing springs). Is their a truck spring or some other vehicle with stiffer springs that I can use.Chevelle springs are 5 1/2" dia. by 12" long.I had spring rubbers in but I had to remove them per the rules. | 2019-04-23T06:58:32Z | http://www.thedirtforum.com/technical/Purestock/001791.html |
The Kookaburra Ghost was one of the best selling cricket bats for most retailers last year. Therefore, it was no surprise then to see Kookaburra expand the whole Ghost range quite substantially.
Keeping with the white and silver colour scheme from the original range, the new additions look really classy and manage to show off the product to great effect.
At the top of the range is the Kookaburra Ghost Pro batting gloves. A sausage style glove, the Ghost Pro offers top levels of protection to the batsmen and thanks to the top quality materials used by Kookaburra, the glove offers excellent protection and comfort too.
It’s commonly known that sausage style batting gloves offer very good levels of protection, but it’s also known that due to the sausage style design with no precut fingers, this style of glove takes longer to break in too.
The Kookaburra Ghost Pro Batting Gloves work well and offers the flexibility batsmen need.
As for sizes, they are available in both small men’s and standard men’s sizes. | 2019-04-18T18:30:56Z | https://vks.com/blog/kookaburra-ghost-pro-batting-gloves/ |
Sharing another on-line chat from an undocumented youth as he gathers support to organize on campus. We’re mobilizing!
you are familiar with the immigration struggle nowadays right?
yea its really sad, and I think we should do something about it.
Would you support immigration reform and if we start organizing in campus to create awareness? | 2019-04-22T12:19:48Z | http://organizedcommunities.org/it-means-the-world-to-me/ |
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I was at the Sydney store a week ago and was assisted by Jack. He was quite knowledgeable, friendly. | 2019-04-22T19:21:06Z | https://www.dhamaalshopping.com/home-page-11/ |
An LLC, or a limited liability company, allows the entity of a corporation to be formed while allowing that entity to have limited financial and legal liability for the actions of that corporation.
AN LLC can include any number of shareholders or partners which includes taxation as a corporation, but they are not technically a corporation. Many times, a limited liability corporation is a sole proprietor company with a single owner.
A single owner may form an LLC in order to protect their finances and personal interests from the corporation. For example, if someone were to sue the LLC and win, the individual owners’ finances could not be included in any awards. In most cases, a judgment against an LLC could not include collection for that judgment from the personal or individual property of any of the individuals that make up the LLC.
Although a limited liability corporation helps to protect those who make up the corporation, there are issues where legal and financial liability is not limited to the corporate entity. For example, if individuals in that corporation commit fraud or misrepresent the corporation, individuals and the company could be liable.
For smaller companies, an LLC is a great way to form a corporation while allowing those within the corporation to have limited financial and legal liability. In addition, the formation of an LLC protects the company itself from being legally and financial liable in most cases. For instance, the owner of an LLC that goes bankrupt would not be liable to the creditors of that corporation in most cases. | 2019-04-22T19:01:19Z | http://business.islaws.com/what-is-an-llc |
As its name implies, CandleVolume charts merge volume into candlesticks. This allows chartists to analyze both price action and volume with one look at the price chart. CandleVolume charts are similar to EquiVolume charts, but offer more information because candlesticks are used instead of high-low boxes. This means chartists can see the open and close for each period, as well as the high and the low. CandleVolume charts can be used just like normal charts. Chartists can look for candlestick patterns as well as classical chart patterns, such as triangles and wedges, to generate signals.
A CandleVolume candlestick consists of five components: open, high, low, close and volume. As with normal candlesticks, the open and close form the body of the candlestick, while the high and low form the upper and lower shadows. Volume determines the width of the candlestick. Wide candlesticks form when volume is high, while narrow candlesticks form when volume is low. The example below shows basic black and white (filled and hollow) candlesticks based on CandleVolume. Wide and hollow candlesticks form when the close is well above the open and volume is high. Wide and filled candlesticks form when the close is well below the open and volume is high. Narrow candlesticks form when volume is relatively low.
Chartists can also colorize candlesticks and volume bars to identify up periods and down periods. The second chart shows a colorized version of the same stock (FDX) over the same period. Just below the chart, in the Chart Attributes section, chartists can check the “Color Prices” box and “Color Volume” box to colorize. A hollow candlestick still means the close was above the open, and a filled candlestick means the close was below the open. The red candlestick means the close was below the prior close, while a black candlestick means the close was above the prior close. The same applies to the red and green volume bars.
When calculating CandleVolume charts, note that volume is normalized to show it as a percentage of the look-back period. For a four-month daily chart, each day's volume would be divided by total volume for the look-back period (four months). As such, the width of each box represents the percentage of total volume for the look-back period. Big volume days take up more space on the X-axis (horizontal) than low volume days. With varying width, this means the date axis is usually not uniform on CandleVolume charts. Some weeks will extend longer because of wide candlesticks, while others will be shorter because of narrow candlesticks. The first chart below shows Pfizer (PFE) with normal candlesticks and a normal X-axis. The second chart shows how CandleVolume changes the X-axis because volume was much higher in June than in prior months.
CandleVolume charts can be used to validate candlestick reversal patterns. A candlestick reversal pattern on high volume carries more weight than a candlestick reversal pattern on low volume. The first chart below shows Transocean (RIG) forming a wide hammer in mid-April. The second chart shows RIG forming a wide bearish engulfing in mid-May. The third chart shows price action after these patterns.
Volume is an important part of chart analysis, especially where it concerns validating support and resistance breaks. An upside breakout on high volume is more bullish than a breakout on low volume because volume is fuel. An upside breakout on high volume shows strong demand that is less likely to fade away. The chart below shows Google (GOOG) with a CandleVolume chart ending on April 19th. Notice how the stock broke above resistance with a wide hollow candlestick. This shows high volume (strong demand) on the breakout. The second chart shows what happened next. Not all setups pay off quite this well, but CandleVolume can help separate the pretenders from the contenders.
CandleVolume charts put price action and volume together for easy visual analysis. Because these candlesticks share the same features as normal candlesticks, chartists can use them to validate candlestick patterns. CandleVolume charts can also be used to affirm a support test or validate a resistance level. A bounce off support with a wide candlestick is stronger than a bounce with a narrow candlestick. The same is true for a decline from resistance. Basically, almost anything done on normal candlestick charts can be applied to CandleVolume charts. Click here for a live CandleVolume chart.
SharpCharts users can find CandleVolume under “Chart Attributes” and “Type”. There is also a volume option directly underneath. Users can choose to have volume off, separate or as an overlay. Volume can also be skipped (off) because it is reflected right on the CandleVolume chart. The example below also shows the check boxes for selecting “Color Prices” and “Color Volume”. | 2019-04-25T22:33:47Z | http://www.stockcharts.com/school/doku.php?id=chart_school:chart_analysis:candlevolume |
This unusual variation of the canteen-shaped container is lightly chiseled with a combination of geometric patterns, birds, and gazelle-like creatures. The distinctive technique and style of surface decoration seen on this vessel associate it with bronzes excavated in far southern China, near modern Vietnam. Under occupation by Han rulers, bronze foundries in this frontier area produced intriguing bronzes in which Chinese styles are blended with those of the native, non-Chinese tribes. | 2019-04-22T05:05:17Z | https://www.artic.edu/artworks/23497/wine-flask-bian-hu?filters=collection_category%3A%221%2FPrints+and+Drawings%2FFrench%22&place_ids=China&classification_ids=vessel |
A huge thank you to the public of Lugano!!!
From the 2 to 9 November, with 10 incredible sold-out performances, more than 10,000 people attended the new show Per te. at the Lac (Lugano Arte Cultura). Our heartfelt thanks for the all the love you showed us. We’d like to remind you that from 18 to 19 February, we will be back in Lugano with La Verità – advance tickets sales available.
“Per te. – almost in paradoxical sense is a hymn to life, to the incredible strength of the theatre, to the imagination and creativity, the basic importance of friendship under the most difficult circumstances of existence”.
“Per te. is studded with moments when large tracts of the lightest fabric flutter, thanks to airstreams measured to the accuracy of a millimetre, at times providing life to souls that refuse to leave, or to the sails of ghost ships that dare to face the worst waves , or even to a large red heart that beats”. | 2019-04-25T06:20:59Z | https://finzipasca.com/en/2016/11/12149/ |
Uniquely-DC has a team of meeting, convention, and event planners who will coordinate all aspects of your professional meetings and events. They will assist you in choosing meeting locations, arranging transportation, and coordinating other details like registration, audio visual, exhibits, food & beverage and much more. The Uniquely DC team has worked in all 50 states and abroad and can also claim to having worked in most all the major convention centers and conference hotels across the United States. They have the experience you need on your team for successful conference, meeting, convention or exhibition execution here in the United States of America.
Please click on the Uniquely DC logo below for more information on their scope of complete meeting planning services. | 2019-04-21T22:47:08Z | http://americathebeautiful.com/meetings.html |
XXL doesn't even begin to describe it. I literally had to take off my cardigan in the first 15 minutes. It's everything you wanted more of from the first "Magic Mike," just with a few unnecessary scenes of dialogue. But they really gave us what we craved: shirtless men, pelvic-thrusting and gyrating to slow R&B jamz, including none other than Mike's signature "Pony." We certainly get an eyefull. I always knew Channing had moves, but Manganiello and Bomer? Out of nowhere! You don't have to see the first "Magic Mike" to appreciate all that XXL has to offer (though you should - it is also entertaining.) Tons of grammies in the audience seemed to be enjoying it too.
Items unfortunately not drawn to scale.
The boys reunite, this time sans Dallas (McConaughey) and head to Myrtle Beach for the male stripper convention. Along the way, they encounter drag queens, lonely housewives and Michael Strahan like you've never seen him before. Live with Kelly and Michael? More like Live with Michael's groin straddling a woman's face.
For every slow and lackluster scene (there are a handful), there is a riveting, sit up in your seat scene, most notably Manganiello dancing to a famous boy band song in a 7-Eleven and Bomer serenading a 40-something housewife to the song "Heaven." Just when you're itching for a new dance number, you get one. Also, I have a new crush on Manganiello. I resisted as long as I could, but this movie will make anyone weak in the knees for him.
This movie has a touch of lady power, though the men clearly shine. Jada Pinkett Smith is slightly annoying as Rome, a nightclub owner who becomes the boys' emcee. Amber Heard (aka Mrs. Johnny Depp) is hardly recognizable and doesn't add much pizzaz to the movie. Andie Macdowell's Southern accent is too forced, which surprised me considering she is from the South. However, her 10 minute scene is one of the more entertaining.
On a more "film studies" note, you can expect the same sepia tones as in the first film. A few scenes were even shot in the shadows, almost so dark that you couldn't see faces or who is speaking. Totally reminisceient of the indie feel of the first "Mike." Don't be shy - go see this film. There's no shame in the gratuitousness these men give. Just enjoy. Then go take a cold shower. | 2019-04-24T15:50:15Z | http://www.cuisineandscreen.com/2015/07/magic-mike-xxl.html |
The Duchess hailed volunteers as the “skeleton” of the country, as she made calls at a telephone hotline founded by Dame Esther Rantzen to help the isolated and lonely.
Saying she was sure The Silver Line made a "huge difference" to the people who now rely on it, she stepped in to speak to 88-year-old Betty Whitcher to hear a poem she had written, share memories of their fathers' service in the war, and wish her happy Christmas.
Speaking at charity's offices, the Duchess, who became a patron this year as she turned 70, said: "It's such a lovely thing to do, especially coming up to Christmas.
"We've got to look after each other."
In a discussion about the problem of loneliness, she said her many visits to charities across the country had taught her of the importance of volunteers.
“Without volunteers, this country would just come to an end," she said. "Volunteers are the skeleton, I think, of Britain.
“And if you talk to them they always say they get back as much as they give. "Not only are you making the other person feel happy, you’re making yourself feel happy."
Asked by Dame Esther what she would say to anyone wondering about volunteering, she added: “Get on and do it. Find something you’re interested in.
"There are so many people who have retired and want to keep the old brain cells ticking over. They can see what they’re interested in and go for it.
"Volunteering is the best thing anybody can do.
The Silver Line runs a helpline, a befriending system - where a volunteer speaks to the same person once a week - and a telephone circle for group conversations about mutual interests such as art and music.
The charity currently takes around 10,000 calls a week, with more than half of callers saying they speak to no-one else.
Designed for the over-55s, organisers are expecting a peak of calls around Christmas and New Year, and are seeking donations to keep lines fully staffed.
Mrs Whitcher, a widow who uses the service, told the Duchess on the telephone that knowing there was someone she could call is "very reassuring".
In a long conversation, she disclosed she had previously sent a poem for Remembrance Sunday to the Duke of Cambridge and Prince Harry, receiving a long letter back.
“Well I can tell them I’ve talked to you," said the Duchess.
"I think it’s such a clever thing to do to write poetry, I’d love to be able to.
Asking Mrs Whitcher to keep in touch and send her any poems she wrote in the future, she added: “You sound wonderful, I hope one day we might meet."
Dame Esther Rantzen, who founded The Silver Line after being widowed herself and realising how lonely an empty house could be even for someone with an able body and active social life, said she had been amazed by the difference one phone call can make.
“It’s really difficult to fundraise for charities about older people," she said.
“Why is that? Is it because of our attitude to older people?
“We are hoping that if people have got any money to spare this Christmas, even though we know it’s tough times, that they may think of those who are going to be completely alone. | 2019-04-24T22:10:18Z | https://www.telegraph.co.uk/news/2017/12/09/got-look-otherduchess-cornwall-urges-public-volunteer-fight/ |
Hollywood travel agencies are incessantly occupied nevertheless it’s likely that it is because of folks as we speak planning to see celebrities and have footage of widespread Hollywood landmarks on their very own. Was established as a travel agent and a tour operator in 1990. Utilizing the companies of a journey agent means you get the customer service you deserve. The primary enterprise activity of the corporate was inbound tourism. If you happen to run into any issues, your journey agent could make calls and usually fix the scenario so you don’t have to waste useful trip time downside fixing.
Travel Brokers Have Contacts – Journey brokers do not get “free” journeys. In this course, you will uncover how this fascinating trip destination satisfies what your clients crave. Self-employment or freelance work is opening up with the recognition of on-line bookings and tailor-made package deal excursions, however this has obvious risks hooked up to it, notably in an business which might expertise downturns and upturns in equal measure.
On-line reserving: Trip packages could be seen and booked on-line by the corporate web site. This web site is just not meant to offer assurance that any specific journey agent meets sure high quality standards or authorized obligations. In case you plan ahead and use a travel agent, you will solely be required to pay a small deposit to substantiate your family’s trip reservations.
We specialize in all-inclusive vacations, group journey, including vacation spot weddings and exclusive hosted teams , cruises, river cruises and way more. We offer a whole range of travel companies for each particular person and company clients and dedicate ourselves to turning those once-in-a-lifetime vacation dreams into realities.
We’re extra than just journey specialists, Our specialties include planning quality customized-made packages at greatest costs, arranging luxurious holidays for VIP shoppers, representing and dealing with travel agents, offering super-yacht charters and aviation ground-handling help. | 2019-04-26T08:09:06Z | https://www.okuhida-yodel.com/maine-travel-companies.html |
Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein localization and dynamics. We engineered a specific binder for fluorescent proteins based on a 13-kDa GFP binding fragment derived from a llama single chain antibody. This GFP-binding protein (GBP) can easily be produced in bacteria and coupled to a monovalent matrix. The GBP allows a fast and efficient (one-step) isolation of GFP fusion proteins and their interacting factors for biochemical analyses including mass spectroscopy and enzyme activity measurements. Moreover GBP is also suitable for chromatin immunoprecipitations from cells expressing fluorescent DNA-binding proteins. Most importantly, GBP can be fused with cellular proteins to ectopically recruit GFP fusion proteins allowing targeted manipulation of cellular structures and processes in living cells. Because of the high affinity capture of GFP fusion proteins in vitro and in vivo and a size in the lower nanometer range we refer to the immobilized GFP-binding protein as GFP-nanotrap. This versatile GFP-nanotrap enables a unique combination of microscopic, biochemical, and functional analyses with one and the same protein.
After the identification of most components of the cell, further insights into their regulation and function require information on their abundance, localization, and dynamic interactions. Green fluorescent proteins (GFPs)1 and spectral variants thereof became popular tools to determine protein localization and, in combination with fluorescence photobleaching techniques, provided unique information on protein dynamics in living cells (1–4). Necessary additional information on DNA binding, enzymatic activity, and complex formation can be obtained with various methods including chromatin immunoprecipitation (ChIP) and affinity purification (5, 6). These methods, however, are hampered by the limited availability of specific antibodies. Those limitations are often bypassed by fusing the protein of interest to specific epitope or protein tags including hemagglutinin, c-Myc, FLAG, or GST (7, 8). Curiously GFP, the most widely used labeling tag in cell biology, is rarely used for biochemical analyses, although various mono- and polyclonal antibodies have been described (9, 10). This may be due in part to limited availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses. An alternative to conventional antibodies are variable single domain antibody fragments, also referred to as VHH, derived from heavy chain antibodies of Camelidae (11). These VHHs, which present the smallest intact antigen-binding units with a molecular mass of about ∼15 kDa are highly soluble and stable and can be efficiently produced in heterologous systems (12, 13). VHH fragments have been used like conventional antibodies for various immunological applications (14–16).
Here we describe a novel application of a 13-kDa GFP binding fragment derived from a llama single chain antibody (17). This GFP-binding protein (GBP) has a small (2.5 × 4.5-nm) barrel-shaped structure and can easily be produced in bacteria. We immobilized the GBP to generate a GFP-nanotrap, which enables a fast and efficient isolation of GFP fusion proteins and their interacting factors for biochemical and ChIP analyses. Moreover we demonstrated that the GFP-binding protein can be fused with structural proteins to ectopically recruit GFP fusion proteins and interacting factors at defined regions in living cells.
Llama immunization, VHH library construction, and selection of the GBP were described previously (17). The coding sequence of the GFP-binding VHH domain was cloned into the pHEN6 (18) vector using the NcoI and NotI restriction sites adding a C-terminal histidine (His6) tag, and chemically competent Escherichia coli BL21 cells were transformed. For expression and purification a 500-ml E. coli culture was induced with 1 mm isopropyl β-d-1-thiogalactopyranoside for 20 h at room temperature. Bacterial cells were harvested by centrifugation (10 min at 5000 × g), and the pellet was resuspended in 10 ml of binding buffer (1× PBS, pH 8.0, 0.5 m NaCl, 20 mm imidazole, 1 mm PMSF, 10 μg/μl lysozyme). The cell suspension was incubated for 1 h at 4 °C in a rotary shaker and then sonified (6 × 10-s pulse) on ice. After centrifugation (20 min at 20,000 × g) soluble proteins were loaded on a preequilibrated 1-ml HiTrap column (GE Healthcare) and purified. The His-tagged GBP was eluted by a linear gradient ranging from 20 to 500 mm imidazole. Elution fractions containing the GBP were pooled and dialyzed into PBS. Protein concentration was adjusted to 1 μg/μl. The yield of purified GBP/liter of bacterial culture was in the range of 10–15 mg.
For bacterial expression and purification of GFP we used the bacterial expression plasmid pRSet5D containing the GFP coding sequence with a C-terminal His6 tag kindly provided by B. Steipe. For mammalian cells we used the following expression vectors encoding different fluorescent fusion proteins: pEGFP-C1, pEYFP-C1, pECFP-C1, GFP-β-actin (all from Clontech), GFP-Lamin B1 (19), GFP-PCNA (20), mRFP-PCNA, GFP-Dnmt1, and GFP-Dnmt1(P1229W) (21). We also used mammalian expression vectors containing cDNAs coding for mRFP1 (22), mCherry, or mOrange (23) kindly provided by R. Tsien. Overlap extension PCR was performed to construct the CFP I147N and the CFP T154M using mutagenic primers CFP-I147N-FW (5′-GAGTACAACTACAACAGCCACAACGTCTATA-3′) in combination with CFP-I147N-Rev (5′-AACGTCTATATCATGGCCGACAAGCAG-3′) and CFP-T154M-FW (5′-AACGTCTATATCATGGCCGACAAGCAG-3′) in combination with CFP-T154M-Rev (5′-CTGCTTGTCGGCCATGATATAGACGTT-3′), respectively, and the flanking primer CFP FW (5′-GATCCGCTAGCGCTACCGGTCGCCACCATG-3′) and CFP-Rev (5′-AACGTCTATATCATGGCCGACAAGCAG-3′). The PCR fragment was cloned into the NheI/BsrGI site of the pECFP-C1 vector. For GBP-Lamin B1, the Lamin B1 was amplified from GFP-Lamin B1 by PCR with primers LaminB1EcoRV#F (5′-CCCCGATATCGGCGACTGCGACCCCC-3′) and LaminB1HisNotI#R (5′-GGGGGCGGCCGCCTAGTGATGGTGATGGTGGTGTTACATAATTGCACAGCTTC-3′). The PCR product was purified, digested with EcoRV and NotI, and ligated into the EcoRV/NotI sites of the GFP chromobody vector. All resulting constructs were sequenced and tested for expression in HEK 293T cells followed by Western blot analysis.
For immunoprecipitation and immunoblotting a mixture of two anti-GFP monoclonal antibodies (clones 7.1 and 13.1) from Roche Diagnostics and an affinity-purified polyclonal anti-GFP antibody raised in rabbit (a kind gift from D. Nowak, Berlin, Germany) were used. Precipitated proteins were detected with antibodies against GFP, mRFP, Lamin B (H-90, Santa Cruz Biotechnologies), β-actin (monoclonal anti-β-actin, Sigma), and PCNA (monoclonal anti-PCNA, clone 16D10). Promyelocytic leukemia protein (PML) bodies were detected with a monoclonal anti-PML antibody (5E10).
1 × 106–1 × 107 HeLa, HeLa H2B-GFP, or HEK 293T cells either mock-treated or transiently transfected with expression vectors coding for GFP, YFP, CFP, mRFP, mCherry, mOrange, GFP-β-actin, GFP-Lamin B1, GFP-PCNA, or GFP-Dnmt1 were homogenized in 200 μl of lysis buffer (20 mm Tris/HCl, pH 7.5, 150 mm NaCl, 0.5 mm EDTA, 2 mm PMSF, 0.5% Nonidet P-40). For extraction of GFP-Lamin B1 or H2B-GFP the lysis buffer was modified by adding 0.5 m NaCl, 1 μg of DNase I, 5 mm MgCl2, and 0.1% SDS. After a centrifugation step (10 min at 20,000 × g at 4 °C) the supernatant was adjusted with dilution buffer (20 mm Tris/HCl, pH 7.5, 150 mm NaCl, 0.5 mm EDTA, 2 mm PMSF) to 1 ml. 20 μl (2%) were added to SDS-containing sample buffer (referred to as input). 1 μg of purified GBP or 2 μg of anti-GFP antibodies were added and incubated for 5–60 min on an end-over-end rotor at 4 °C. For pulldown of immunocomplexes 25 μl of an equilibrated mixture of protein A/G-Sepharose (Amersham Biosciences) were added, and incubation continued for 60 min. After a centrifugation step (2 min at 5000 × g at 4 °C) supernatant was removed, and 2% was used for SDS-PAGE (referred to as flow-through). The bead pellet was washed two times in 1 ml of dilution buffer containing 300 mm NaCl. After the last washing step the beads were resuspended in 2× SDS-containing sample buffer and boiled for 10 min at 95 °C.
1 mg of purified GBP was covalently coupled to 1 ml of N-hydroxysuccinimide-Sepharose (GE Healthcare), according to the manufacturer's instructions, generating the GFP-nanotrap. Subsequently 50 μl of the GFP-nanotrap was transferred to a 1-ml column (MoBiTec) and preequilibrated with 5 ml of dilution buffer. Protein extracts of GFP-producing cells were prepared as described above, and the soluble protein fraction was loaded onto the column. The protein solution (1 ml) passed through the column at 100–200 μl/min flow rate. Subsequently the column material was washed (two times) with 1 ml of dilution buffer containing 300 mm NaCl, and bound proteins were eluted with 100 μl 0.1 m glycine, pH 3.2. 2% of the input and flow-through and 10% of bound material were resuspended in 2× SDS-containing sample buffer and analyzed by SDS-PAGE and Coomassie Blue and by immunostaining.
Soluble protein extracts of HEK 293T cells producing GFP-Dnmt1 or GFP-Dnmt1(P1229W) were prepared, and immunoprecipitation was performed as described above. Beads containing the GBP-GFP-Dnmt1 complexes were washed extensively in dilution buffer containing 300 mm NaCl, and after centrifugation the beads were resuspended in 500 μl of assay buffer (100 mm KCl, 10 mm Tris, pH 7.6, 1 mm EDTA, 1 mm DTT). Beads were washed (two times) in assay buffer, and after centrifugation (1000 × g for 1 min) 30 μl of methylation mixture (0.1 μCi of S-[3H]adenosylmethionine (Amersham Biosciences), 1.67 pmol/μl hemimethylated double-stranded 35-bp DNA (50 pmol/μl), 160 ng/μl BSA) were added. As a positive control 2 μg of recombinant purified DNA methyltransferase I (DNMT1) was used. Incubation was carried out for 2.5 h at 37 °C, and the reactions were spotted onto DE81 cellulose filters. Subsequently filters were washed (three times) with 0.2 m (NH4)HCO3, once with H2O, and once with 100% EtOH. After drying at 80 °C the filter was transferred into a Mini-Poly-Q vial with 5 ml of Ultima Gold LSC Mixture (PerkinElmer Life Sciences), and each sample was measured for 1 min in a scintillation counter (Beckman LS1801). A sample without enzyme addition was used as negative control.
For chromatin immunoprecipitation experiments, formaldehyde was diluted to 1% in serum-free medium, and 15 ml were added to monolayers of C2C12 and C2C12/HMGA1a-GFP cells for 10 min at room temperature. The cross-link was stopped by adding 1.5 ml of 1.25 m glycine. After removal of the medium, cells were washed (two times) on plates with cold 1× PBS, scraped off, and washed again in cold 1× PBS and once with hypotonic LSB buffer (10 mm Hepes, pH 7.9, 10 mm KCl, 1.5 mm MgCl2). Cells were resuspended in 2.7 ml of LSB buffer and lysed by adding 300 μl of 20% Sarkosyl. The chromatin was carefully layered onto a 40-ml sucrose cushion (LSB buffer plus 100 mm sucrose) and centrifuged (10 min at 4 °C at 4000 × g). Supernatant was removed, and the chromatin was resuspended in 2 ml of TE (10 mm Tris, 0.5 mm EDTA, pH 8.0) and sonicated (Branson sonifier 250-D, 35% amplitude, 2 min in 1-s intervals). For each immunoprecipitation, 500 μg of the nucleoprotein were adjusted with volume of 1× NET (50 mm Tris, 150 mm NaCl, 0.5 m EDTA, 0.5% Nonidet P-40). 10 μg of two anti-GFP monoclonal antibodies (clones 7.1 and 13.1; Roche Diagnostics), a mouse IgG1 isotype control, and 5 μg of GFP-nanotrap were incubated overnight at 4 °C. The purification of co-precipitated DNA was performed as described above. Real time PCR was performed with the Light Cycler instrument (Roche Diagnostics) using a ready-to-use “hot start” reaction mixture (FastStart DNA Master SYBR Green I; Roche Diagnostics). Real time PCR analysis was performed according to the manufacturer's instructions using the same parameters as described previously (24). The list of primer pairs is given in supplemental Table 1.
Ideally the subcellular localization and binding dynamics of fluorescent fusion proteins should be complemented with biochemical data on interacting factors, enzymatic activity, and DNA binding properties (Fig. 1). To generate a nanotrap for fast and efficient purification of GFP fusion proteins we used a small recombinant 13-kDa GBP comprising the epitope recognition domain of a heavy chain antibody raised in an alpaca (Lama pacos) against GFP (17). The GBP was fused with a C-terminal histidine (His6) tag, produced in E. coli, and purified by IMAC (supplemental Fig. S1A). Subsequent gel filtration analysis showed a single elution peak at about 13 kDa indicating that the GBP can easily be produced and purified as a stable monomer (supplemental Fig. S1B). For a first functional analysis we tested the binding capacity of the GBP to purified GFP. Equimolar amounts of both proteins were mixed and incubated for about 20 min. Subsequent gel filtration analysis showed that the GBP and its antigen rapidly assemble into a stable, stoichiometric complex with a corresponding native molecular mass of 41 kDa (supplemental Fig. S2). We then tested the ability of GBP to precipitate its antigen from cell extracts and directly compared its performance with mono- and polyclonal anti-GFP antibodies. GBP and anti-GFP antibodies were added to protein extracts of HEK 293T cells expressing GFP. Precipitated proteins were separated by SDS-PAGE and visualized by Coomassie Blue or immunoblot analysis (Fig. 2A). After precipitation with GBP only two protein bands (GFP at ∼28 kDa and the GBP itself at ∼13 kDa) were detectable, whereas additional bands corresponding to the light and heavy chains of the denatured IgGs as well as unspecifically precipitated proteins could be observed in immunoprecipitations with the mono- and polyclonal anti-GFP antibodies. The quantitative comparison of precipitated GFP in relation to antibody input showed that the GBP is clearly more efficient than established mono- and polyclonal antibodies (Fig. 2A and supplemental Fig. S3).
Outline of possible GFP fusion protein applications with the GFP-nanotrap.FRAP, fluorescence recovery after photobleaching; FRET, fluorescence resonance energy transfer.
One-step purification of fluorescent proteins.A, immunoprecipitation of GFP. Left panel, comparison of GBP with mono- and polyclonal anti-GFP antibodies. Protein extracts of GFP-producing HEK 293T cells were subjected to immunoprecipitation with GBP, mono-, or polyclonal anti-GFP antibodies. Throughout (A–C) aliquots of input (I), flow-through (FT), and bound fraction (B) were separated by SDS-PAGE and visualized either by Coomassie Blue (top) or by immunoblot analysis (bottom). Precipitated GFP, denatured heavy (hc) and light chains (lc) of the IgGs, and the GBP are marked by arrows. Right panel, column-based purification with the GFP-nanotrap. GFP-containing protein extracts were loaded on a column containing GBP directly coupled to Sepharose (GFP-nanotrap), and bound proteins were eluted. B, one-step purification of GFP fusion proteins with the GFP-nanotrap. Representative images of HeLa cells producing GFP-β-actin, GFP-Lamin B1, GFP-PCNA, or H2B-GFP are shown (upper panel). Cells were lysed, and GFP fusion proteins were one-step purified with the GFP-nanotrap. C, co-precipitation of GFP fusion proteins and interacting factors. HeLa cells coexpressing either GFP-Dnmt1 or a deletion mutant of Dnmt1 (GFP-Dnmt1Δ-PBD) together with the red fluorescent version of PCNA (mRFP-PCNA) were analyzed by live cell microscopy. Representative confocal images of two cells in early-to-mid S phase are shown (scale bar, 5 μm). Subsequently cells were lysed and subjected to immunoprecipitation with the GFP-nanotrap. wt, wild type.
Conveniently GBP complexes can be immobilized and precipitated with protein A-agarose just like conventional antibodies (Fig. 2A, left panel). However, to prevent elution of the GBP itself and to avoid any interference with subsequent analyses we covalently coupled GBP to N-hydroxysuccinimide-Sepharose beads generating a stable GFP-binding matrix (GFP-nanotrap). This GFP-nanotrap allowed a very fast (5-min) column-based purification of GFP from total cell extracts yielding a single protein band without any visible unspecific protein contamination (Fig. 2B, right panel). Moreover the immunoblotting analysis revealed a quantitative precipitation of the antigen visible as depletion of GFP from the flow-through fraction.
To analyze the specificity of GBP we performed immunoprecipitation with a set of different fluorescent proteins. Besides GFP itself, GBP recognizes the yellow variant YFP but not CFP or any derivatives of DsRed like mRFP, mCherry, or mOrange (supplemental Fig. S4). To further characterize the binding properties of GBP we tested different salt, temperature, and pH conditions. First, we increased the NaCl molarity of the binding buffer from 0.15 to 2 m and found that GBP precipitates its antigen even under high salt conditions (supplemental Fig. S5A). Second, we tested different incubation temperatures and observed quantitative precipitation of GFP even at 65 °C (supplemental Fig. S5B) demonstrating the temperature-stable folding and binding of GBP. Third, we tested the elution of bound epitope at different pH conditions. GFP was stably bound in the range from pH 4 up to pH 11. Lowering the pH to 3.2 caused quantitative release of GFP providing a fast and efficient method to elute bound antigen complexes from the GFP-nanotrap (supplemental Fig. S5C).
To investigate whether GBP also precipitates GFP fusion proteins we chose four well characterized constructs from different subcellular compartments: GFP-β-actin (25), GFP-Lamin B1 (26), GFP-PCNA (20), and H2B-GFP (27). Cells transiently or stably expressing the fusion proteins were analyzed by confocal microscopy to determine the subcellular localization of the GFP fusion protein (Fig. 2B, upper panel). Subsequently cells were harvested, and GFP fusion proteins were precipitated with the GFP-nanotrap followed by Coomassie staining and Western blot analysis (Fig. 2B, lower panel). The results show that GBP efficiently precipitated N- or C-terminal GFP fusion proteins from different subcellular compartments and structures.
One of the most interesting applications of the GBP is the possibility to correlate subcellular localization, dynamics, and complex formation of specific proteins and mutants thereof. We had previously identified a deletion mutant of Dnmt1 lacking the PCNA interaction domain (Dnmt1Δ-PBD) (28). In comparison with wild-type Dnmt1 (GFP-Dnmt1) the fluorescent labeled mutant (GFP-Dnmt1Δ-PBD) showed no colocalization with the red fluorescent PCNA (mRFP-PCNA) during S phase (Fig. 2C, upper panel). We then compared this observation from living cells with biochemical data. Cells were lysed immediately after microscopic analysis and subjected to immunoprecipitation with the GFP-nanotrap. Immunoblot analysis showed that mRFP-PCNA co-precipitates with GFP-Dnmt1 but not with GFP-Dnmt1Δ-PBD (Fig. 2C, lower panel). The unbound mRFP-PCNA fraction reflects the transient nature of the Dnmt1-PCNA interaction during S phase (21). In addition, we also detected endogenous PCNA co-precipitating with GFP-Dnmt1 but not with GFP-Dnmt1Δ-PBD, demonstrating that GBP can also pick up interacting endogenous proteins. These results show that the GFP-nanotrap is suitable to identify (even) transiently interacting factors and cell cycle-dependent complexes. Taking advantage of its avid and specific binding we now routinely use the GFP-nanotrap to identify interacting factors by MALDI mass spectrometry.
A critical question in live cell studies is whether the fluorescent fusion proteins are enzymatically active and have biochemical properties similar to their endogenous counterparts. For fast determination of enzymatic activity we chose fluorescent fusion constructs of the murine Dnmt1, which are actively investigated in our laboratory. With live cell microscopy we could not distinguish the active GFP-Dnmt1 (wild type) and an enzymatically inactive version, GFP-Dnmt1(P1229W) (Fig. 3, upper panel). After visualization of the fluorescent Dnmt1 constructs in nuclei of mammalian cells both proteins were one-step purified from cell extracts with the GFP-nanotrap (Fig. 3, middle panel) and directly assayed for enzymatic measurements. GFP-Dnmt1 showed a specific DNA methyltransferase activity similar to that of purified recombinant DNMT1 (Fig. 3, lower panel). The comparison with the catalytically inactive GFP-Dnmt1(P1229W) revealed that the endogenous DNA methyltransferase activities of mammalian cells were efficiently removed by this quick one-step purification. These results demonstrate that the GFP-nanotrap is a highly efficient tool for quick and reliable biochemical characterization of fluorescent fusion proteins.
GFP fusion proteins retain their enzymatic activity after purification with the GFP-nanotrap. Representative images of nuclei from HeLa cells show the endogenous Dnmt1, GFP-Dnmt1, or catalytically inactive mutant GFP-Dnmt1(P1229W) (top). Soluble protein extracts containing GFP-Dnmt1 or GFP-Dnmt1(P1229W) were one step-purified with the GFP-nanotrap and analyzed by SDS-PAGE followed by Coomassie Blue staining (middle). DNA methyltransferase activity of purified GFP fusion proteins (∼4 μg each) and recombinant DNMT1 (∼2 μg) were determined (bottom). n = 4; error bars show standard deviation.
To investigate whether the GFP-nanotrap has the required specificity to function in chromatin immunoprecipitations we performed ChIP experiments using a mouse myoblast cell line stably expressing a GFP fusion of the high mobility group protein A1a (HMGA1a-GFP).2 We analyzed the promoter regions of the Interleukin 6 and Igf-binding protein 1 genes that are known to preferentially bind HMGA1a (29–32). 500 μg of formaldehyde-cross-linked nucleoprotein were subjected to immunoprecipitation with the GFP-nanotrap and compared with commercially available GFP-specific antibodies. Only the GFP-nanotrap showed a reproducible 3-fold enrichment of promoter fragments over distal sites. This preferential binding of HMGA1a-GFP at the specific promoter sites was not detectable with conventional GFP-specific antibodies (Fig. 4). The parental C2C12 cells not expressing HMGA1a-GFP showed no specific enrichment of any sequences in comparison with the isotype control (data not shown). These data confirm the high specificity and sensitivity of the GFP-nanotrap.
ChIP analysis with the GFP-nanotrap. For ChIP experiments, 500 μg of C2C12/HMGA1a-GFP cells were subjected to immunoprecipitation using 5 μg of GFP-nanotrap, 10 μg of anti-GFP antibody (monoclonal), and 10 μg of mouse IgG1 isotype control, respectively. Co-precipitated DNA was quantified by real time PCR analysis using primer pairs of the IgfBP1 and Il6 promoter regions and indicated downstream regions (distal region 1/2). Results obtained for the Interleukin 6 and IgfBP1 promotor are shown in dark gray. Column heights indicate the relative enrichment obtained in two independent experiments as the difference between PCR values obtained with the GFP-nanotrap and anti-GFP antibodies over mouse IgG1 isotype control antibodies.
These experiments show in vitro applications of the GFP-binding protein. In a second step we explored potential in vivo applications and placed the coding region of the GFP-binding protein at a defined position within the cell by fusing it to a structural component of the nuclear envelope. We fused the GBP with Lamin B1 (GBP-Lamin B1) to generate a cellular nanotrap at the nuclear lamina. As described above, GBP efficiently recognized and bound YFP but not the blue variant CFP in vitro (supplemental Fig. S4). To compare these biochemical data with binding specificity in living cells we transfected HeLa cells with GBP-Lamin B1, GFP, and CFP and determined the distribution of GFP and CFP with respect to the GFP-nanotrap positioned at the nuclear lamina. Confocal microscopy showed that only GFP was exclusively localized at the nuclear lamina, whereas CFP showed a strikingly different, disperse distribution throughout the cell (Fig. 5A). Sequence alignment of GFP, YFP, and CFP showed that CFP differs from the two other fluorescent proteins at two relevant amino acid positions (Fig. 5B). To define the epitope recognized by GBP we introduced single mutations reverting stepwise CFP to GFP. We found that the exchange of isoleucine at position 147 to asparagine (I147N) restored binding to the GFP-nanotrap, whereas mutating methionine at position 154 to a tryptophan (T154M) did not restore any detectable binding (Fig. 5C). This demonstrates the high selectivity and specificity of the GBP in vitro and in vivo even allowing detection of single amino acid substitutions. Finally we analyzed whether the GFP-nanotrap can be used to manipulate endogenous factors and cellular structures. As a target we chose the endogenous PML. The PML is organized in nuclear PML bodies, which play a role in regulation of gene expression, apoptosis, DNA repair, and proteolysis (33, 34). We could detect 10–30 PML bodies by immunofluorescence staining of PML in HeLa cells where PML bodies are evenly distributed throughout the nucleus (data not shown). In transiently transfected cells PML bodies are highlighted by the green fluorescence of the GFP-PML (35) fusion protein and immunostaining with a specific antibody against PML (Fig. 6A). Upon expression of GBP-Lamin B1 green fluorescent labeled PML bodies were exclusively found at the nuclear rim, and subsequent antibody staining confirmed the depletion of endogenous PML from the nuclear interior (Fig. 6B). These results demonstrate the potential of the GFP-nanotrap not only to recruit GFP fusion proteins but also interacting endogenous factors.
Specificity of the GFP-nanotrap.A, in HeLa cells expressing GFP, CFP, and GBP-Lamin B1 GFP is exclusively localized at the nuclear lamina, whereas CFP is dispersedly distributed. B, amino acid sequence alignment of GFP, CFP, CFP I147N, and CFP T154M. Non-conserved residues are marked in red. The crystal structure of GFP (36) is shown, and the asparagine at position 147 is displayed as a blue sphere using PyMOL Version 0.99 (DeLano Scientific LLC). C, soluble protein extracts of HEK 293T cells expressing GFP, CFP, CFP I147N, and CFP T154M were subjected to one-step purification with the GFP-nanotrap. Input (I) and bound fractions (B) were separated by SDS-PAGE and analyzed by Coomassie Blue staining and by immunoblotting with anti-GFP antibody.
Targeted manipulation of nuclear structures with the GFP-nanotrap. HeLa cells producing GFP-PML either alone or in combination with GBP-Lamin B1 are shown. Illustrating outlines are shown on the left. A, GFP-PML is assembled into endogenous PML bodies shown by immunofluorescence staining with an anti-PML antibody. B, coexpression of the GBP-Lamin B1 together with GFP-PML leads to a depletion of PML bodies from the nuclear interior shown by the absence of PML bodies in the nuclear interior after immunostaining with an anti-PML antibody.
One challenge of the postgenomics era is the effective integration of genetic, biochemical, and cell biological data. This integration has in part been impeded by the simple fact that different protein tags are used for different applications. Here we present a GBP as a simple, robust, and versatile tool for biochemical analyses of GFP fusion proteins and even functional studies in vivo. The small and stable GBP has several decisive advantages over the conventional, 10 times larger, mono- and polyclonal antibodies. First, the GBP can be produced in bacteria in unlimited quantities and with reproducible quality and can easily be coupled to beads or matrices. Second, the high affinity of single chain GBP allows short (5–30-min) incubations to isolate fluorescent fusion proteins and even transiently bound factors from different cellular compartments. Third, its small size of about 13 kDa minimizes unspecific binding and entirely avoids contamination by heavy and light chains of conventional antibodies (50 and 25 kDa) that normally interfere with subsequent analyses.
The specificity of the GFP-nanotrap was further underlined by ChIP experiments with stable cells expressing a HMGA1a-GFP fusion. Analyzing DNA binding at two gene loci with the GFP-nanotrap we detected a preferential binding of HMGA1a at the promoter regions that was far less evident with established anti-GFP antibodies.
In conclusion, this versatile GFP-nanotrap together with the widespread use of fluorescent fusion proteins now enables a fast and direct correlation of the subcellular localization and mobility of fluorescent fusion proteins with their enzymatic activity, interacting factors, and DNA binding properties combining cell biology and biochemistry with mutual benefits. Unlike conventional antibodies, the GFP-nanotrap can also be used for functional studies in vivo. We demonstrated that GBP can be fused with cellular proteins to set a GFP-nanotrap at a defined subcellular site to either ectopically recruit GFP fusion proteins and their associated factors or deplete them from their native surroundings. In one example we anchored the GBP at the nuclear lamina to capture nuclear bodies. Multiple other applications are possible because any GFP fusion protein can be combined with any cellular anchor point. The use of GBP as a nanotrap in living cells opens up an entire spectrum of new types of functional studies to probe and manipulate cellular processes and structures.
We thank A. Gahl and D. Nowak for excellent technical assistance; A. Jegg, J. Helma, R. Kleinhans, and K. Schmidthals for practical support; G. Längst and K. Dachauer for enzyme activity measurements; and R. Hock for the C2C12 HMGA1a-GFP cell line.
↵ 1 The abbreviations used are: GFP, green fluorescent protein; GBP, GFP-binding protein; ChIP, chromatin immunoprecipitation; VHH, variable domain of heavy chain antibody; HEK, human embryonic kidney; IgG, immunoglobulin G; YFP, enhanced yellow fluorescent protein; CFP, enhanced cyan fluorescent protein; DsRed, Discosoma genus red fluorescent protein; mRFP, monomeric red fluorescent protein; PCNA, proliferating cell nuclear antigen; H2B, histone H2B; Dnmt1, DNA methyltransferase I; PBD, PCNA binding domain; HMGA1a, high mobility group protein A1a; Igf, insulin-like growth factor; PML, promyelocytic leukemia protein.
↵ 2 J. Brocher and R. Hock, manuscript submitted.
↵* This work was supported by the Center for NanoScience, by the Nanosystems Initiative Munich, and by grants from the Deutsche Forschungsgemeinschaft. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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Just over a year ago I tasted a couple of dozen clarets from 1996 and found them to be ripe, with classy fruit as well as good tannins and acidity. At the time I wrote “…high quality vintage with considerable cellaring potential. The wines as a group show very good balance and good structure. The merlot-dominated right bank wines also showed well I thought, though perhaps the fruit quality is better in the cabernet-based wines”. This was an interesting chance to re-assess the vintage, though only a few of the wines that were on tasting back in April 1999 were also included here. Those are indicated, with a link to the original note.
The tasting was not blind. .
This has a deep, dark ruby colour. Very claretty nose, distinctly cedary edge to deep plum fruit. Real Christmas cake aromas. Quite cool, classy fruit on the palate. This has a gamy quality too and lots of sinewy tannins. There is some creamy blackcurrant in there. The finish shows quite highish acidity, suggesting this might be better with food. Quite good value.
Quite a deep, vibrant ruby with warmth at rim. More new oak and a much riper quality of fruit. Much sweeter nose with more obviously ripe, concentrated fruit. Lots of blackcurrant and plum with chocolate and coffee bean nuances. Medium-bodied, there is a little leanness on the palate with firm, chewy tannins gripping the finish along with toasty oak, but just a touch austere? Has structure, so should come together, but a little awkward at the moment.
First bottle corked. Bright, dark ruby. Opaque core. Very dark cedar wood and creamy dark chocolate nose with dried fruit, prune aromas. Lots of spice too and there is a blackcurranty note in there. Palate shows much greater concentration of fruit than previous wine; much riper too with a blue/black character of bitter-edged plumskins and cherries. Very long with spice and sweet oak bolstered by ripe, chewy tannins. Very long. Fine and should cellar well.
Medium ruby colour, just gaining warmth. Nose a touch stalky. A bit dumb, with rather flabby fruit aromas though there’s a gamy, meaty quality too. On the palate more definitely lacking fruit with tannins dominating along with some old tasting, cedary oak. Hints of sweet fruit and game, but this is not very well balanced.
Very deep and dark ruby/crimson. Loaded with coffee bean, charry oak and tobacco on the nose. Little violet notes too and some blackcurrant and plum fruit. Nice fruit on the palate which is quite cool and classy with grippy tannins. Medium-bodied, it has pretty good balance with integrated acidity. Good value, and with a touch more fruit would have been excellent.
Second wine of Pichon-Baron. Very dark blackish/crimson colour. Sulphury bottle stink blows off (just about) to reveal a slightly rubbery, stewed black fruit. Not sure this isn’t mildly corked either? Palate has good weight, but again fruit quality is a bit baked. Grippy tannins and some dry, mineral and ash flavours. Not a convincing bottle.
Very dark ruby. Deep, dark toasted oak aromas, almost liquoricy. Beneath the fruit is slightly baked again, blackberry and spiced plum. Lots of concentration here. The fruit is dark and bitter-edged, but it is ripe and the acidity and firm, juicy tannins are balanced. Good length and purity into the finish. Very good.
Second wine of Léoville-Las-Cases. Medium/dark vibrant crimson/black. Restrained and elegant, classy nose with cedary, savoury oak but plenty of ripe blackcurrant fruit and a mineral quaity. Lovely sweetness of fruit on the palate. Very ripe, pure cassis, quite firm and grippy but also pure, juicy and fresh. Fine balance into a long, oak-infused finish which hints at a lovely future for this wine. Excellent.
Tarry, dense, blackish colour. Obviously huge extraction. Lots of charry new oak on the nose with pencil-shaving aromas adding savouriness to ripe, crunchy blackberry, damson and plum. Silky mouthfeel with a palate of sweet, dark, bittersweet fruit and liquorice. Firm tannins are gripping powerfully though acidity is moderate. Very good indeed.
Medium/deep ruby fading to orange at rim. Nose is a little rubbery, even slightly dank, with a hard cedariness that doesn’t have enough freshness of fruit to really balance. Better on the palate, still not displaying enough quality of fruit to be really enjoyable at present, but there are blackcurrant flavours as well as some coffeeish oak and pretty good length. I last tasted this wine a couple of years ago and enjoyed it a lot more then, so a confusing picture. Perhaps a slightly out of condition bottle.
Quite a deep glowing gold. Fine botrytis nose loaded with honeyed figs and notes of ripe melon and quince. There’s a lightness about the nose too, a flowery acacia hint, but then another layer is like butterscotch and caramel. On the palate this has fine melon fruit with a candied pineapple note and citrus flavours. Medium-bodied, and only medium sweet, there is also an intriguing aniseed note in the finish. Very elegant and fine. Very good. | 2019-04-18T22:47:53Z | https://wine-pages.com/tasting-articles/reassessing-the-1996-clarets-10-wines-0600/ |
More than 50 people had to be rescued from a fatal fire in an apartment block in Paris on Monday.
Claire spoke about her escape from the fire, which was one of the most deadly in the French capital in years.
Investigators believe the fire may have been started deliberately. | 2019-04-20T19:08:27Z | https://www.bbc.co.uk/news/av/world-europe-47138450/paris-fire-survivor-jumped-across-balconies-to-escape |
7 Benefits from Time Apart | Lucinda Secrest McDowell – "Encouraging Words"
I’ve often encouraged others to “come apart before you fall apart.” As 2019 dawned, I realized it was my turn to do just that.
When I chose to “hibernate” for the month of January, my intention was to deliberately live quietly, offline, and mostly out of touch. I continued much of my work and daily life responsibilities, but did relocate part of the month – primarily for my husband’s recovery from recent brain surgery.
There is no one formula for such a time of desperate respite. Because mine came at the first of a new year, it was for me a time to pray, plan and purpose for the days ahead. But it can also look like a weekend retreat or even one day away at a local monastery. Whatever form your time apart takes, God can use those moments to restore your soul.
Rest is good for both body and brain. Most of us know this, but often resist tending to our own needs. Richard Foster observed, “I have discovered that the most difficult problem is not finding the time but convincing myself that this is important enough to set apart the time.” In our currently sleep-deprived culture, health experts agree that “a good night’s sleep helps foster both mental and emotional resilience. On the other hand, chronic sleep disruptions set the stage for negative thinking and emotional vulnerability.” (Harvard Health) Late last year I began to manifest being overstressed and exhausted. As I listened to my husband’s neurologist speak of how important it is to rest both body and brain, the thought occurred, Perhaps I need to recover from his brain surgery. “When our brain enters the rest circuit, we don’t actually rest, we move into a highly intelligent, self-reflective, directed state. And the more often we go there, the more we get in touch with the deep, spiritual part of who we are.” (Dr. Caroline Leaf, “Switch on Your Brain”) Time apart gave me the freedom to sleep, nap, rest my mind and actually become more creative and energetic at the same time.
I find wisdom in embracing the paradox of productivity during such a season. Truth is that (even whilst on a book deadline) I had to realize that though few words were written during my time apart, many words were being lived out and others marinating for eventual release. I often think of this reminder from John Ruskin: “There is no music in a rest, but there is the making of music in it. In our whole life-melody the music is broken off here and there by ‘rests,’ and we foolishly think we have come to the end of the tune. Not without design does God write the music of our lives. But be it ours to learn the tune, and not be dismayed at the ‘rests.’ They are not to be slurred over nor to be omitted, nor to destroy the melody, nor to change the keynote. If we look up, God Himself will beat the time for us. With the eye on Him, we shall strike the next note full and clear.” As with much of life, there is often more going on than is outwardly evident.
My prayer life becomes richer when I have the freedom of fewer “to do” lists competing for my attention. For someone who occasionally struggles with focus while praying, a time apart can provide (mostly) uninterrupted spaces and seasons to pray continually. I also love to pull out treasured prayer books and lift up words prayed through the centuries. While at the beach I turned to this prayer from St. Aidan of Lindisfarne (590-651 AD) “Leave me alone with God as much as may be. As the tide draws the waters close in upon the shore, Make me an island set apart, Alone with You, O God, holy to You. Then with the turning of the tide Prepare me to carry Your presence to the busy world beyond, The world that rushes in on me, Till the waters come again and take me back to You.” This month I also enjoyed many of the liturgies from “Every Moment Holy” — “In our days away let us play together. Let us laugh together. Let us be moved to speak such meaningful words as ought to be spoken among family and friends. Let us linger long at tables and drink deeply of one another’s company, enjoying each for who they are with the steady pressures of our ordinary days now lifted.” (Douglas McKelvey, 2017) My prayerful conversations with God were quieter but deeper.
Words and songs of gratitude became my mantra. Over and over I thanked God not only for all He continues to give and do and be for me, but for the absolute undeserved grace gift of this time apart. Australian novelist Morris West suggests that at a certain age our lives simplify and we need have only three phrases left in our spiritual vocabulary: “Thank you! Thank you! Thank you!” That is exactly how I feel in this season of my life, but it sometimes takes a new perspective to recharge me back to constant praise. Though I’ve always read at least one Psalm a day, I’ve been inspired to dig even deeper in this amazingly poetical and praise-worthy book. A dying woman once told my author friend Barbara Mahany “If you love the life you have, please, please, please practice gratitude. Wake up every morning acknowledging just how much beauty is in your world. Pay attention to it, honor it and keep your heart and your eyes wide open. You won’t regret it.” (“The Blessings of Motherprayer”) Yes, Thank You, Thank You, Thank You!
Would you like to embrace some of these amazing benefits of a time apart?
I hope you can schedule into your new year some intentional time apart. And I’d love to hear the benefits you discover in the process.
Cindy, this blog drew me right back to my core, where I live with God alone in intimacy. | 2019-04-22T12:04:25Z | https://lucindamcdowell.wordpress.com/2019/02/05/7-benefits-from-time-apart/ |
Round Table, India organized their Annual General Meeting in Unawatuna, Sri Lanka from 30th June to 1st July 2018. The event was successfully planned and managed by Colombo Conferences.
The team arrived from the airport and relaxed at the Jungle Village, Unawatuna in the afternoon. Later that evening, A welcome dinner with shisha and cocktails was organized at the beautiful Comaran Beach Club. The night was based on the theme “Fire & Coal”. Light music, cocktails, bohemian shisha huts and minimal decor by the calm, beautiful unawatuna beach made it a memorable one.
The second day, the AGM held followed by interactive programs, cocktails and dinner. The event was based on Coconut theme from decor, props, ambiance, setting & arrangement to the dinner menu which was mostly with coconut dishes.
Once the AGM was over, the DJ took over the night and the pool party continued till dusk. | 2019-04-19T01:09:32Z | http://colomboconferences.com/round-table-india-hosts-their-annual-general-meeting-in-unawatuna-sri-lanka/ |
This contemporary and classically styled block of six flats was manufactured, erected and insulated by Timber Frame It for local developers Orchard Estates Ltd.
The robust party wall detail included a combination of two 90mm timber stud walls filled with Warmcel 500 separated by a 50mm wall cavity. This particular type wall was specifically requested by the client and endured a number of sound and watertight tests which all passed with flying colours.
We used Cellecta DECKfon ScreedBoard 28 for the flooring on this project. Being a new material used by TFI, we researched and vigorously tested the product and was impressed with its benefits, it passed the sound test and is now product we offer within our products. | 2019-04-23T20:21:07Z | https://www.timberframeit.co.uk/portfolio-item/flats-orchard-court-little-common-east-sussex/ |
This handy and small box contains a mix of small candies in Orange, Green Apple, Strawberry, and Lemon flavored chewy candies. In 1975 Morinaga introduced a new fruit flavored candy called Hi-Chew, which have since become a cultural icon in Japan. Their goal was to create an irresistible candy which at first and feels and tastes like bubble gum, but becomes softer and softer after chewing. Hi-Chew candies are much more juicy and fruity than regular candies. No matter what Hi-Chew flavor you choose, you're in for a long-lasting, mouth-watering treat. Enjoy! | 2019-04-19T21:07:10Z | https://www.japancandystore.com/hi-chew-chewy-mini-candy |
These settings can be changed in the admin panel > Settings > Campaign Settings > "Various" section. Please see below for explanation on each setting.
Make address optional in detailed registration (detailed registration only): If this is checked the address fields during registration won't be validated.
Make address required: Checking this option will require users to enter their complete address and phone number in the "Account Details" popup-window.
Show RSVP pop after "Detailed Registration": If RSVP task is added as a "HQ Startup Guide" in the "HQ Tasks", it will be shown even for campaigns that are using the "Detailed Registration" option.
Don't allow returning users to import email templates: Check this to prohibit registrants from previous year to import email templates into this year's campaign.
RSVP task never gets crossed out: Check this to ensure the RSVP task in the HQ never gets crossed out even if RSVP task is completed.
Hide sub-registrants in team list by default: Regardless of this setting, you can always show or hide individual sub-registrants by accessing their record in the People app.
Email address is optional for offline donations: Check this to allow email address as an optional field when member submits offline donations.
Donation form should open in lightbox popup-window on personal pages: Uncheck this if the Donate button on personal pages should direct to the donation page instead of opening a lightbox on the page.
Note: The phone number field and custom fields from the donation form are not presented in the lightbox. Leave this unchecked if you need to collect that information.
All new personal pages are delisted by default: Check this for new personal pages to not show up in site-wide lists or search. They will still be accessible to anyone via direct link. Page access can be changed by the admin (per page) after registration.
All new team pages are delisted by default: Check this for new team pages to not show up in site-wide lists or search. They will still be accessible to anyone via direct link. Page access can be changed by the admin (per page) after registration.
Require campaign admin to activate every new personal page: Admins will be able to see all pages and members will be able to see their pages even when inactive.
Require campaign admin to activate every new team page: Admins will be able to see all pages and team captains will be able to see their team pages even when inactive.
Allow fundraisers to see their donors' email address: Check this to allow members to see their donor's email address in their Fundraising report.
Allow fundraisers to see their donors' billing address: Check this to allow members to see their donor's billing address in their Fundraising report.
Allow team captains to see team members' email addresses and see the above enabled info for ALL team donations: Regardless of this setting, team captains will be treated as the member for general team donations. Check this setting if you want team captains to see ALL the team donors' enabled info - even for donations submitted towards another team member.
Require users to RSVP: RSVP step will not have a skip option. Members will also be redirected to the RSVP step until it's completed.
Add a "subscribe to newsletter" checkbox to the contact form: Check this to add a "subscribe to newsletter" checkbox to the contact form. | 2019-04-18T13:15:26Z | https://confluence.rallybound.com/display/KB/Campaign+Settings+-+Various |
The biggest US-based genealogy company will acquire the biggest Swedish genealogy company. Subscription genealogy site Ancestry.com has agreed to buy Genline, a subscription site featuring virtually all Swedish church records, for about $6.7 million, according to Global NewsWire. Read more about the transaction here.
The biggest US-based genealogy company will acquire the biggest Swedish genealogy company. Subscription genealogy site Ancestry.com has agreed to buy Genline, a subscription site featuring virtually all Swedish church records, for about $6.7 million, according to Global NewsWire.
This entry was posted in Ancestry.com, Genealogy Industry, Genealogy Insider, International Genealogy Research. Bookmark the permalink. | 2019-04-25T18:35:14Z | https://www.familytreemagazine.com/articles/genealogy-websites/ancestry/ancestry-com-to-buy-genline/ |
Vallejo Symphony is committed to bringing vibrant, live music to students in schools and the concert hall.
Vallejo Symphony musicians, guest artists, and Maestro Taddei are committed to bringing live music into classrooms across Solano County, creating participatory experiences that engage and thrill students of all ages. Through interactive discussions, performances, and clinics, we aim to help our community give a every student a chance at music.
Vallejo Symphony is also committed to giving students a real concert experience once a year in the Empress.
The Vallejo Symphony Trio performs in elementary and middle schools each year.
Marc Taddei introduces Mozart! 800 students from four cities were invited to the performances at the Empress.
Our goal is to expose children to different genres of music by playing works from different time periods and teaching them about music and composers, with the hope of engaging them in musical performance and planting the seeds of a life-long love of music.
If you’re interested in having Vallejo Symphony partner with your school, or would like to support these community-enriching programs, please get in touch! | 2019-04-26T10:27:42Z | https://vallejosymphony.org/education-1/ |
Essays aren’t just composed on a few subjects but are also presented in various modes. Realistically, possibly sort of composition will generally tackle both similarities and variations. If you determine to examine a topic from the physical along with the social studies, elements, like measurements, methods, relationship studies, and data about the experiments conducted have to be contemplated. Now you’ll furthermore wish to predetermine the particular regions the article may concentrate on. »A function can get modern only when it is first postmodern. Here, I’ll teach you the means to write a very powerful outlines and a number of examples of focus getters for you personally next article! Below are some amazing article issues that kids may possibly discover straightforward to compose. Also the writers should have a pattern to perform on so that they are going to write a productive essay. An composition isn’t about fiction, is regarding the fact. Counterfeit documents are essays at which writer pulls out the primary thesis and synopsis of a specific document, after which it composes an article in his or her own type.
Insert a no cost close, such as for example truly or sincerely, and hit the return key 3 x.
Comparison documents are inclined to concentrate on likenesses, although contrast documents concentrate on variations. custom essay A comparative composition is applied to compare two notions or theories. Creating style is among the most crucial aspects in writing. Meeting essays ensure it is feasible that you use individuals as your own sources in contrast to publications. A enormous literature was composed on this special problem Perhaps a small should to be said about spritiual poverty. Instructors must also provide the youngsters proper instructions for every form of article they have to write. | 2019-04-19T03:14:01Z | http://fjell-bygg.no/erwin-chargaff-and-the-history-of-genetics/ |
Of the four chambers of your heart, the most significant is your left ventricle, which receives oxygenated blood from your lungs via your right ventricle and then diffuses it to the cells throughout your body. When you train consistently, your left ventricle actually increases in diameter, meaning a larger chamber to accommodate a greater volume of oxygen-rich blood. A concurrent adaptation is an increased total blood volume, with more red blood cells to transport oxygen.
Cardiac tissue is a specialized type of muscle, called smooth muscle, that contracts involuntarily. Like other muscles, the strength of contraction of heart muscle depends on the size and strength of individual muscle fibers. When you make your heart pump harder through regular exercise, cardiac muscle fibers increase in size, leading to a denser, stronger ventricular wall. The stronger muscle tissue enables you heart to contract with greater force, ejecting a higher volume of blood per stroke.
When your heart health is at an enhanced state from exercise, it runs more smoothly and conserves energy. Your heart rate is lower at rest and at relative levels of exercise intensity. This is due to a combination of greater available blood volume and increased force of contraction. In other words, each time your heart beats, a greater volume of blood enters your circulatory system, and the heart needs to beat less frequently to meet your body’s oxygen demands. The combined product of stroke volume and beats per minute is called cardiac output.
Regular exercise changes the cells in your skeletal muscles at the same time that your heart adapts. When both the skeletal muscle cells and the cardiac muscle cells are exposed to consistent overload, you are able to utilize more oxygen at higher intensities, measured as VO2 max, or maximal oxygen consumption. Individuals with a high VO2 max typically outperform those with a lesser training status. This may equate to being able to run, swim or bike farther, at a faster pace.
Like many things in life, the benefits of exercise to heart health have their limits. Excessive cardiac training may lead to increased risk of arrhythmias like atrial fibrillation. It may also lead to scarring of your cardiac tissue, inhibiting optimal function. Moderation in intensity and duration of exercise may be the best option for training a healthy heart.
At the heart of all change is education. The more you know and understand about your body and how lifestyle choices affect your health, the better equipped you will be to prevent and reverse the deadly precursors to heart disease. As a fitness professional, it is up to you to educate your clients and help them to make better choices. W.I.T.S. offers certification and continuing education courses that will fill the gaps in your understanding about heart disease. To get started, check out our courses in Nutritional Concepts, Exercise Program Design, Group Exercise Certification and Certified Personal Trainer.
Cleveland Clinic: Can Too Much Extreme Exercise Damage Your Heart?
mercola.com: Long-Distance Running: One of the Worst Forms of Exercise There Is. | 2019-04-18T23:27:32Z | https://www.witseducation.com/fit/blog/youre-all-heart-5-ways-exercise-changes-your-most-vital-organ/ |
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Like many sites, “cookies” are used by this Site to collect information. You can instruct your browser to refuse all cookies or to indicate when a cookie is being sent. However, if you do not accept cookies, you may not be able to use some portions of this Site. | 2019-04-20T00:45:50Z | https://healthliteracyhub.org.au/terms-privacy/ |
Craftsman Coffee Table – Here’s some pictures of design ideas for your home furniture design related to craftsman coffee table. We collected the images from various sources to provide the best inspiration for you. The image that we serve is high quality and high resolution, with the hope that you can clearly see the detail of inspiration about craftsman coffee table.
We hope, some of the furniture design below can add to the beauty of your home. You can find related images related to Craftsman Coffee Table on this website by discovering the category section, or the related posts below. So, take your time and find the Lovely Craftsman Coffee Table pictures informed here that suitable with your needs.
Hopefully, the pictures about Craftsman Coffee Table that we have provided this can be useful for you all. Don’t forget to share this post with your friends to help your friend in finding their inspiration. Creative greeting from our and Thank you for visiting. Remarkable Craftsman Coffee Table Woodsmith Shop Americas Favorite Woodworking Tv Show. Fabulous Craftsman Coffee Table Craftsman Style Coffee Table Mission Style End Table Robertoboat. Brilliant Craftsman Coffee Table Craftsman Coffee Table Rustic Wood Cocktail Table Custom Made. Amazing of Craftsman Coffee Table Craftsman Coffee Table Bungalow Style Arts Craft Rustic. Awesome Craftsman Coffee Table Living Room Furniture Mission Furniture Craftsman Furniture. | 2019-04-19T04:40:20Z | http://www.furniturefavourites.com/3621/craftsman-coffee-table/ |
Mobile virtual network operators, MVNOs are mobile service providers who do not own the network infrastructure on which they operate. This implies that the MVNO will get into an agreement with a Telco to obtain certain services at a negotiated/wholesale price; the MVNO will in turn offer retail services to its customers independently.
has so far licensed three MVNOs in Kenya. The licensed operators are Finserve Africa (a subsidiary of Equity bank), Tangaza Mobile (associated with Mobile Pay Limited) and Zioncell Kenya (associated with Mo-De). The three licensed MVNOs in Kenya have partnered with Airtel Kenya. The three licensed operator will focus on offering Mobile money, SMS, Data and voice services.
The biggest influence for operating a MVNO in Kenya is competition in the mobile money space which is currently dominated by safaicoms Mpesa. The biggest beneficiary of all these developments in the Mobile money sector will surely be the wananchi through reduced money transfer charges, easy access to Loans from your phone and a variety of providers to choose from.
Technology Airtel Kenya, Communications Authority of Kenya, Finserve, mobile telephony, MVNO. permalink. | 2019-04-22T22:30:40Z | http://infosasa.com/mvnos-in-kenya/ |
Crossfit Kisco is the name of a training facility. Crossfit is a type of training that involves very high intensity workouts. We want the color scheme to be balck with lime green (florescent green, bright green)and white. We want the initials of the company to be either CK or CFK. We were thinking about some sort of graffiti involved. That's just one idea tho. Again, this company represents a high intensity training facility. | 2019-04-24T23:47:17Z | https://www.designcontest.com/logo-design/crossfit-kisco/brief/ |
Pets are wonderful companions that can make any place feel like home. Sometimes, however, pets can get territorial over pieces of furniture, or leave a trail of shed fur all over a clean couch or bed. When these inconveniences arise, it's important to teach your pet to stay off the furniture. Learning how to break these bad habits can help you train your pets to stay off the furniture for good.
Get your pet a comfy bed. Before you can train your dog or cat out of sleeping on the couch or bed, you'll need to give your pet a comfortable alternative. You probably won't have much luck keeping your pet off the furniture if you don't provide some kind of alternative. In fact, some pets are better off sleeping on soft furniture, due to arthritic pain or discomfort sleeping on the floor. A dog or cat bed can help provide your pet with a comfortable place to sleep without causing you any inconvenience.
Get your cat a condo. If your cat continually climbs on furniture and it's causing you problems, one of the best alternatives you can provide your cat is a cat condo. Cat condos are multi-level structures that allow cats to climb, jump, and sleep in one of the "rooms" or on one of the platforms. These indoor structures allow your cat his own place to sleep while also allowing him to climb and get exercise.
Designate one piece of furniture for pets. If your pet isn't satisfied with sleeping in a dog or cat bed, consider designating one piece of furniture as a pet-friendly spot. It could be an old arm chair or a love seat that you don't really use anymore - anything that your pet will take a liking to. However, it's important to reinforce to your pet that he can't climb on other pieces of furniture.
Some animals may have a hard time understanding, especially at first, why one piece of furniture is okay but others aren't. The key is consistency. Any time your pet tries to climb up onto a "humans only" piece of furniture, lead him off that furniture and onto his designated chair.
Use treats as an incentive. Once you provide your pet with an alternative, "pet-friendly" piece of furniture, you may need to train your pet to use that piece of furniture instead of humans-only furniture. Treats can be very effective for this. Leave treats on the dog/cat bed, in the cat condo, or on the pet-friendly piece of furniture to make these pieces of furniture highly desirable to your pet. Any time your pet tries to get on a humans-only piece of furniture, make him get down, and toss a treat onto the piece of furniture or bedding you've provided for him.
Restrict your pet's access from the start. If you've recently brought home a new pet, or you plan to in the near future, it's best to restrict your pet's access to furniture from day one, if being on the furniture is an issue for you. It will be much harder (though not impossible) to break this habit once your pet has learned that it's acceptable to be on the couch/chair/bed, so curbing this behavior from an early age is crucial.
Keep food away from the furniture. It's possible that your dog climbs on the couch because he knows you snack there, and there might be crumbs in the cushions. Or maybe your cat climbs on the kitchen counter because she knows you put her food bowl up there from time to time. Whatever piece of furniture your pet is climbing on, there's a chance he's come to associate that location with food. Keep food away from the furniture you're trying to train your pet away from, and promptly clean up any messes you make on or near that furniture.
Use double-sided tape. One way to train your pet out of using furniture (and keeping your pet off, even when you're not home) is by making the furniture less comfortable. Buy some cheap place mats and cover one side of them with double-sided scotch tape. You can then lay these sticky mats, with the adhesive side up, on whatever counters or couch cushions your pet is drawn to, or just apply double-sided tape directly to the furniture. The sticky sensation will be extremely uncomfortable, yet it won't harm your pet, and if you're using place mats it won't damage your furniture, either.
Use aluminum foil. Aluminum foil is another simple furniture deterrent that will make furniture loud and uncomfortable, and therefore undesirable, without harming your pet or damaging your furniture. Simply lay sheets of aluminum foil across couches, chairs, and counter tops. You can also use an upside down plastic carpet runner across your couch and chair cushions.
Block access to furniture. A simple way to keep pets off your couches and recliners is by laying other furniture, like folding chairs, across the cushions. This will remove all temptation by making the furniture completely inaccessible to your pet, yet it's still simple enough for you to easily remove when you want to sit on the couch or chair.
Create a gentle booby trap. One way to train your pet out of climbing onto furniture, especially when you're not home, is to lay a gentle trap. A good example of a harmless (yet effective) booby trap is to stack a bunch of empty soda cans on the furniture. Be sure the empty cans are well-rinsed, to ensure that no sticky residue gets on your furniture. You can build a little pyramid, either on a couch cushion or on the headrest above an armchair, and if your pet tries to climb up the toppling cans will frighten him off the furniture. Over time it may startle him enough to stay off the furniture permanently.
Buy a deterrent device. If you don't want to create your own furniture blockers, there are a number of commercially-available furniture deterrent devices on the market. The Snappy Trainer consists of a large paddle attached to a sensitive trigger, which can detect when an animal has climbed onto the couch or chair cushion and causes the whole device to snap up in the air. The SSSCAT, another motion-activated device, shoots a blast of air at your pet if he crosses the motion sensor while climbing onto your furniture. The Sofa Saver is a device you lay across the couch, and if a pet climbs onto it, the device activates a noisy alarm that will scare away your pet. Each of these devices can prove to be highly effective because they startle your pet without causing any harm, and they can be used even when you're not home.
Observe your pet on the couch. The off command, though highly effective, does require you to be present and observe your pet climbing onto off-limits furniture. This technique works better for dogs, since dogs tend to respond to verbal commands better than cats. When you observe your dog climbing onto a couch or chair he's not supposed to be on, rather than physically removing him (which may elicit an aggressive reaction from him), teach him the "off" command.
Say "Off" and give a treat. As soon as you observe your dog on the couch or chair, use a calm-yet-stern voice to say, "Off." Then hold a small treat in front of his face, and pull the treat (with your dog's nose in tow) slowly away from the couch or chair and down to the floor.
Praise your dog, and repeat. As soon as your dog gets down from the furniture, offer him verbal praise and give him the treat you led him away with. Start out giving him treats every time he climbs down, then gradually taper off how frequently you give treats with the "off" command. Eventually you should be able to command your dog to get off the furniture simply by saying, "Off."
Give your pet more exercise. One possible reason your cat may be climbing on furniture is because he isn't getting enough exercise and stimulation on a daily basis. If you cannot provide him with a cat condo, get your cat a scratching post, and use interactive toys that allow him to chase, run, and jump. This should tire him out, removing some of the necessity to climb, scratch, and explore your furniture.
Use chemical deterrents. Cats and dogs have a natural aversion to anything that smells or tastes like citrus or bitter apple. You can use this to your advantage as you train your pet to stay off counters or furniture. Use citrus-scented cleaning products on the counters you want to keep a cat off of, or spray a little citrus-scented oil or bitter apple on couches to keep pets off. (Just be sure that you don't ruin your furniture by spraying oil extracts on the cushions!) Dogs and cats are both repelled by these scents, and will stay away if you make certain environmental elements smell like these undesirable scents.
Crate or confine your pet. If your pet tends to climb on furniture when you're not home (evidenced by lots of hair or scratch marks on furniture), consider getting a crate for your dog when you're not home, or confining your pet(s) to a designated area, like a bathroom or kitchen. You can also use baby gates to block access to certain rooms or pieces of furniture. Just be sure your animals have access to food and fresh, clean water, and aren't exposed to uncomfortable temperatures, no matter where you confine them.
My dog knows it's wrong to jump on the sofa, but still does it when we're not there. He cannot be confined to a room or crate, as he will destroy things. What do I do?
The chances are he associates any punishment for jumping up with you being present rather than the action of jumping on the sofa. This is why he jumps up when you're not there. Consider if it might be simplest just to put a dog blanket on the sofa when you aren't there. Alternatively, teach him two new commands: "Up" for jumping up on the sofa, and "Floor" for getting off. By putting the action on cue, you can then 'forget' to give him the "Up" command, which means he's a lot less likely to do so of his own accord.
Is there a spray to keep cats off furniture?
Some people recommending daubing furniture with essentials oils that cats dislike the smell of (such as eucalyptus or lemon). Always test the furniture for color-fastness first. Otherwise, a remote deterrent such as motion-activated compressed air is the best thing, as this gives the cat a remote punishment that she won't link with your presence and is therefore effective at all times, not just when you're around.
Do cats like to sleep with their owners?
This depends on how well socialized the cat is and their individual personality. But as a general rule, a cat that likes people will love the warmth and comfort of sleeping next to their owner.
How do I stop my dog from getting on the couch when I'm not home?
This is tricky if you allow the dog to get up there when you're home. It is too confusing for the dog if he's allowed up when you're home, but not when you're out. So the first step is to have zero tolerance of the dog getting on the couch at all. If you can't trust the dog while you retrain him, then consider crate training to keep him confined (and out of trouble) when you aren't home.
Train the dog to jump down on command. The "Floor" cue works well. If the dog is on the furniture, lure him off with a treat. As soon as his paws hit the floor, say "Floor" and give the treat. Keep practicing this. You can also teach "Up" for him to jump onto the furniture. Although this sounds a bad thing to do, by putting "Up" on cue, it does help with the "Floor" training and often lessens their spontaneous jumping up.
Why does my more submissive Chihuahua mix dog sometimes pee on my bed or on my couch and lately anything that is new to her when she is medically fine?
If you have other dogs, then she is trying to tell them that she is the leader and to stay away from those areas but if she is the only pet, then she just wants to mark territory or get attention.
Can I put my cat's litter box and food bowl in the same room?
How do I break my dog's habit of sitting on the furniture?
Every time she is off the couch, reward her. When she is on the couch, make a sound which can be associated with whenever she doesn't do the right thing.
Will apple cider vinegar keep my cats off my furniture?
In my experience, no. However, it really depends on the individual cat.
How do I teach a deaf kitten to stop scratching the back side of the sofa? Also, how can I train her? I also have another senior cat and 2 dogs.
Get a scratching post and introduce it to her using treats and/or catnip to encourage her to use it. Usually when they have something else to scratch, they'll stop scratching the furniture. Treats should work for training her in general, but try to do the training in a separate room away from your other pets.
Reward your pet for doing as he is told.
Be consistent in your training.
Give your dog or cat plenty of toys to play with.
Don't reward your dog for hopping up onto the couch, rather reward them when they hop off.
Double-sided tape is highly effective, but be aware that it will get very dirty rather quickly. You'll need to change the tape on a regular basis. Tape may also be difficult to remove from furniture, especially if your furniture is made of wood.
Never punish your pet for climbing on furniture. Your furniture is designed to be comfortable, and your pet simply wants the same comfort that you do. Yelling at your pet for wanting to join you on the couch will only confuse and frighten him. Providing him with a comfy, pet-friendly alternative will be much more effective than yelling or punishing.
To keep pets off your furniture, provide them with an alternative like a dog bed or cat condo so they have something to sit or lie on. You can also stick double-sided tape to a place mat or piece of cardboard and leave it on the furniture as this will make it very uncomfortable for your pet. Alternatively, try laying out a sheet of tin foil, which will make the furniture loud and unappealing for your pet. Additionally, you could spray the furniture you don't want your pet to climb on with deterrent spray to keep them away from it. For tips on how to teach your pet the "off" command, keep reading!
Thanks to all authors for creating a page that has been read 527,906 times.
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"I thought there were some really good ideas in this article." | 2019-04-25T10:46:51Z | https://www.wikihow.com/Keep-Pets-off-the-Furniture |
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The information below is provided to keep you informed about the Forest Preserve District of Will County's ongoing or recently completed land acquisition, restoration and development projects.
Project Status: Estimated completion in July 2019.
Project Location: From 111th Street to Rockwell Lane, just north of 119th Street in Naperville and Plainfield.
Project Description: The Forest Preserve will continue the process of converting the underutilized road into a trail. This project includes the design and construction of 0.85 miles of trail. | 2019-04-23T20:47:40Z | https://www.reconnectwithnature.org/conservation/land-preservation-and-improvement-projects/normantown-trail-improvement-project |
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Related Posts of "Medical Billing Modifiers Examples" | 2019-04-18T20:51:31Z | https://myfountainonline.com/medical-billing-modifiers-examples/ |
Walk into a dealership and a customer expects to see cars on the showroom floor, smiling faces of sales associates and possibly a service department employee. But for many dealerships, there’s another addition: a dealership dog.
At Kingston Nissan in New York, customers walking in the front door were immediately drawn to the site of an English bulldog, Ozzie, with a balloon tied to his collar (so customers wouldn’t trip over his sleeping form, general manager Tom Gordon says). Ozzie has since passed, but his legacy at the dealership lives on.
At Stoudt-Ross Ford, Heather Mae Wetzel is often greeted as “Ike’s mom” by customers who know Ike, her five-month-old Bernese Mountain dog.
Pets at the dealership—just a cute gimmick? Think again. For roughly 10 years, not only was Ozzie a customer service greeter, he also starred in the dealership’s commercials, adding antics to the advertisements like when he wore a cape and rescued a woman tied to train tracks.
Ozzie entered the dealership mere months after it opened and was a vital point of customer retention from the beginning, Gordon says.
Wetzel got Ike in October 2018 and immediately started bringing him into Stoudt-Ross Ford in Valley City, N.D. Wetzel, the business development manager, says she took pictures of him and put him on the dealership’s Facebook page.
“As long as he makes someone smile during the day, we’ve done a great job!” Wetzel says.
From the puppy stages onward, the reality is that pets, in all their glory, can be an extremely effective tool to add personality and become memorable to customers.
Part of the allure to bringing Ozzie into the shop was to make it feel like a family atmosphere. Ozzie was adopted by Gordon and his wife six months after the dealership opened its doors. At the time, the dealership was just starting out in the market and underperforming, Gordon says.
In 2018, the dealership produced over 100 percent more business than it had when it started in 2005. And, Gordon has reason to believe that Ozzie was partly responsible for that increase.
At Stoudt-Ross Ford, Wetzel and Mike Rhinehart, general manager, bring in their dogs to the office in order to break the ice when meeting new people. While Wetzel and Rhinehart bring in the pets in order to bring a smile to someone’s face after he or she was in an accident, they remain aware of situations in which people don’t like dogs. In those instances, Wetzel will take her dog and Rhinehart’s gordon setters into a separate room with a closed door to make the customer feel comfortable.
Gordon immediately started using Ozzie in commercials for the business. The cable television advertisements showed Gordon either holding Ozzie or having Ozzie sit next to him.
Some of the ads featured only Ozzie with the name of the dealership across the screen and Ozzie riding a skateboard or snowboard. Gordon featured Ozzie in the ads as a way to make the dealership stand out and for the public to gain awareness of the fact the business supports animals.
In addition to spreading the name of the shop as a customer- and pet-friendly company, the advertisements had an easy theme when they occurred during the national dog holidays like National Dog Day, National Pet Week and National Pet Month. During these days, the dealership ran one type of advertisement that featured pictures of Ozzie in order to keep the name top-of-mind with the customer and connect with people who own pets.
At Stoudt-Ross Ford, Wetzel says she sees more customers walking back in the door after having bought a service and inquiring after her puppy.
After Ozzie’s unexpected passing in 2015, Gordon and the rest of the staff created “The Ozzie Fund,” where Gordon and the staff picked five local, no-kill animal shelters to which to donate.
Kingston Nissan advertised the charity fundraiser to the public and the customers who had come in to the dealership inquiring after Ozzie. For every car the dealership sold, the business donated to the shelters. The dealership asked for public donations, as well. The news of Ozzie’s passing had made its way into the community, so the campaign started when his death was fresh in the public’s mind and lasted about three months during the year. In 2018, Gordon decided to lower the timeframe to three weeks. The dealership raised roughly $12,000 for the shelters that year.
For a dealership looking to turn one animal into a charity cause, Gordon recommends starting by picking local charities that are nonprofit, in which the dealership can donate money to. | 2019-04-21T02:13:48Z | https://www.fixedopsbusiness.com/articles/3011-a-far-fetched-marketing-idea |
Taylor has been designing software since the age of 10 when he developed a website for his church and began writing Casio™ Graphing calculator apps. In 2009 Taylor earned degrees in Math Education (5-12) and Computer Science from Saint Olaf College.
Taylor has spent the past five years teaching high school in Saint Paul, MN. He has worked with youth in urban and rural schools, in public, ALC, and charter schools. Over the last four years Taylor has helped to bring Saint Paul Conservatory for Performing Artists (SPCPA) charter school from 50th percentile to the top 1.3 percentile in mathematics in Minnesota.
Professional Resumes can be found below. | 2019-04-18T16:16:14Z | http://reecemath.com/about.php |
A long time to produce, but worth it: version 0.3.0!
* And much work by Marten Richter to keep the windows code up to date alongside the linux stuff, Thanks Marten!
Many things have been forgotten off this list I am sure. Many thanks to all who contributed.
The next few versions hopefully won't take so long as they won't get bogged down in such major protocol rewrites.
Apologies for those wanting particular features in this version that aren't there, we both know who you are.
I forgot to mention (and strangely had no complaints about )... The l10n directory distributed in vdr-vompserver-0.3.0.tar.gz needs to be copied to [vdr-config]/plugins/vompserver. (Copy the l10n directory, not just the contents). Then the languages will show up again in the client. | 2019-04-25T07:20:29Z | https://forum.loggytronic.com/index.php?topic=349.0 |
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A MISSING PAINTING. A THRILLING CHASE. A CONMAN AT HIS PEAK.
THE HEIST OF A LIFETIME.
Vagh Pratap Singh, aka the Captain, is conman extraordinaire. From transporting illegal merchandise and stealing cars to breaking safes, he’s done it all. But now, in his fortieth year, he’s ready to retire.
So when Udhham Kumar, a crooked politician from Uttar Pradesh, commissions the Captain to track down and steal Poppy Flowers, a Vincent van Gogh painting that has been smuggled into India after it went missing from a museum in Egypt in 2010, the Captain knows that he’s found his last and biggest case.
But a dangerous gangster is now in possession of the painting, and the Captain is being followed by Udhham Kumar’s ruthless associates, greedy for both money and power. As the odds against him begin to stack up, the Captain realizes that his last heist might not be as easy as he’d imagined.
Vish Dhamija’s latest, an ingeniously crafted page-turner about an audacious modern-day heist, is crime fiction at its finest.
Vish Dhamija is the bestselling author of seven crime fiction books. He is frequently referred to in the press as the ‘master of crime and courtroom drama’ in India. In August 2015, at the release of his first legal fiction, Deja Karma, Glimpse magazine called him, ‘India’s John Grisham’ for stimulating the genre of legal fiction in India, which was almost non-existent before his arrival on the scene. The Heist Artist is his eighth novel. Vish lives in London with his wife, Nidhi. | 2019-04-20T20:09:14Z | https://harperbroadcast.com/2019/01/29/harpercollins-india-presents-the-heist-artist-by-vish-dhamija/ |
Created in 1980 as a private label brand of Seiyu department stores in Tokyo, retail giant MUJI sells everyday necessities including clothing, household goods and food.
After becoming independent from Seiyu in 1989, it initially established a global presence by opening stores in London and Hong Kong.
MUJI now boasts around 400 stores in Japan and 300 across the rest of the world, with the number of stores outside Japan expected to exceed its domestic operations in the next few years.
In addition to physical stores, MUIJ created an online store and a smartphone application, MUJI Passport, for customers in Japan. Since its creation in 2013, MUJI Passport has attracted over 5 million users, and acts as a tool for communication between the customer and company. Since 2015 the MUJI Passport app has expanded beyond Japan, into China and Taiwan.
As physical stores make up 90% of MUJI’s total sales, they are a major focus of the digital marketing department. MUJI Passport works to bring customers into physical stores, thereby increasing sales.
As Chief Marketing Technologist Kosuke Hamano explained, the digital marketing department had conducted trial and error to find methods of increasing the number of customers visiting their stores. Previously, there were promotions conducted using the MUJI web site or email newsletters, but any increase in customers tended to be temporary or limited to a certain area. As a result of this trial and error, the MUJI Passport application was developed.
In addition to its function as a rewards card, where customers gather points from buying items or checking in at a store, the MUJI Passport application also offers information useful to the customer, including the ability to look up items in stock in a nearby store.
The data collected from the MUJI Passport service is used for trade area analysis. Through GPS information from the app, plotted onto a map for analysis, the data that area managers previously dealt with through feeling and intuition can be utilised more effectively with tangible statistics. This data can then be used to lead customers to sales, and determine effective locations for expansion.
"We have conducted trading area analysis using other tools in the past, but it was complicated and difficult to operate, as it was designed to be used by data scientists. It was difficult for non-technical staff such as store developers and area managers to use on a daily basis. We had also previously considered implementing a Geographic Information System, but the cost was a big hurdle," Chief Marketing Technologist Hamano recalls.
"We have millions of customer records collected from MUJI Passport. It was important that it had an intuitive interface for non-technical staff to use, and that we could quickly plot information onto a map. Considering these requirements and performance balanced with cost, we came to MotionBoard Cloud. We chose to go with the cloud solution because our IT staff are limited, and we could trust the security and management of the server," continues Hamano.
The decision to purchase was made in June 2015, and WingArc developed dashboards based on feedback from area managers and store developers.
"We can now see all the important information in one screen. Currently the dashboards are aimed at area managers and we are expanding to include individual branch owners," says Hamano.
Using MotionBoard Cloud, the area manager can see where customers are coming from to get to stores in the areas they manage. Also, the store developer can analyse the potential effect of opening a new store on others in the district, and can confirm after opening whether the results were as predicted.
Currently, MUJI is using MotionBoard alongside two other data analysis tools. The other two are a tool for data scientists to flexibly analyse hundreds of millions of records, and another to analyse whether the products developed by the merchandising department are selling as intended. This is due to Hamano's belief in having the most suitable person do a job.
"It’s not what tool is being used, it’s who will use the tool that is important. If the tool isn’t suitable for the person using it, they won’t continue with it. For the area manager and store developer, MotionBoard Cloud was the most suitable tool due to its intuitive interface."
MUJI's company strategy has been rooted in listening and responding to customer needs. Thus, the visualisation of customer actions and business areas was a natural step.
Beginning with MUJI passport, MUJI is opening channels for communication with the customer. The end goal is to maximise "customer time", so that the customer will think of MUJI not just at the time of purchase, but before and after as well. MUJI Passport and MotionBoard Cloud are crucial to MUJI's plan to execute on this strategy.
MUJI had already collected millions of customer data records from the MUJI Passport rewards app, but had no environment with which to effectively analyse this data.
Decision making was solely done by area managers directly viewing the data.
Data could not be effectively utilised.
MUJI uses MotionBoard Cloud to aggregate customer behaviour data and perform commercial area analysis. This brings data that area managers need to see together into a single dashboard. Where decisions were previously based only on the area manager’s intuition, these are now backed by concrete statistics.
The area manager can estimate their market size and the specific characteristics of the area they are responsible for, and store development personnel can plan for store openings and predict the impact on existing stores. | 2019-04-22T18:57:35Z | https://wingarc.com.au/customers/case-studies/muji/ |
One of the biggest obstacles in many burn injury cases is identifying the likely cause and origin of the fire. The “area of origin” for a fire can be a broad area, like a room in a commercial building. The “point of origin” is the smallest area that can be identified as the origin of the fire. After identifying the origin of a fire, investigators look for things that could have served as an ignition source.
In determining the cause and origin of a fire, experts look to the evidence at the scene. There are typical fire patterns which can be seen by looking at the way that certain items at the scene are burnt, discolored, etc. However, fire scenes are dynamic. Critical evidence can be displaced or damaged while rescue workers struggle to put out the fire. It is important that the proper experts are retained by your attorney and sent to the scene as soon as possible to photograph and gather important evidence. If evidence is taken from the scene, experts must ensure that the evidence is properly secured. | 2019-04-19T06:29:40Z | https://hhklawfirm.com/cause-origin/ |
In this new environment, how do you see a list of the threads you're watching (or subscribed to pre-update)? I was able to re-locate a thread of interest and I noticed I had the option to Unwatch. Sounds like they're still tracked. Apologies in advance if it's something obvious I'm missing. Thanks.
Wow. Pretend you never read that. Thanks! | 2019-04-24T05:50:36Z | https://www.troublefreepool.com/threads/subscribed-watched-threads.177179/ |
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Anda juga akan mendapatkan pengingat jika check-in online sudah bisa dilakukan untuk penerbangan Anda. Begitu penumpang duduk di kursi Enterprise Class, mereka bisa segera menikmati fasilitas hiburan dengan konten movie, musik, dan permainan terbaru. By the point I had plugged my destination – a bar I selected mostly for its straightforward title – into my rideshare app and wove by virtually empty streets to the other aspect of Boston Widespread, the Patriots led by only a subject goal.
A passion, no matter it might be is certainly a fun activity and a stress buster. Setelah Anda berhasil melakukan test-in on-line, Anda akan mendapatkan boarding cross. Hal ini bukanlah momen yang membanggakan untuk Batik Air, terlebih saat itu Batik Air masih terhitung sangat baru di industri penerbangan. Menu makanan di Enterprise Class Batik Air cukup beragam, dari makanan ringan hingga makanan utama, yang penuh dengan cita rasa khas Indonesia.
Once you hear about travel and leisure vacations what thought or creativeness will get into your head? Allow them to gather the entire current deals and reductions on airfare and resorts world wide. Setelah menyetujui Kebijakan Check-in On-line, isi knowledge test-in Anda. Chairs whose occupants had jumped out of them in pleasure have been like MINI Coopers wedged between pickup vans: they tricked you into considering you had discovered an open spot before sorely disappointing you. | 2019-04-24T06:21:47Z | https://www.blackpoolbeachhotels.com/the-significance-of-motels-and-lodging-in-selling-tourism.html |
I love candles and candle holders. There are so many different designs for a candle holder; I am going to look at some of the basic variations such as votive, wrought iron, hurricane and crystal holder. In the beginning candles were made of tallow, very smoky and burnt very quickly. Later polish was used, but was expensive. Candle light holders came into being to hold the candle and protect the user's hand, but also to collect the wax and reuse it. Nowadays the simple candle holder has become an ornamental item and is utilised in interior design. They are available in many forms, styles and colors. There is something to charm to everyone from votive holders to ones composed of wrought iron, or mug and crystal.
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As some of you may know, I am into 360/3D/VR Marketing. The idea is simple. A camera takes a video/pictures of the 360° around it and then you can look around as if you were there. Like most marketing techniques there are the best practices to follow to achieve the best results.
Have you ever seen a 360 video or picture? How long were you there? A few seconds? A minute? A year ago or so I saw my first (and only) 360 ad. IT WAS AWESOME! I wasn't even interested in what they were selling, but spent about 5 minutes on their ad! How much would you pay for 5 minutes in front of a potential client?
Even though I wasn't a passing customer I did like the ad, but I can't remember the name of the company that made the ad! Just because something is deemed cool doesn't mean people will remember it. MySpace for example! Even in 360 videos you need to put your brand various times in the video or picture!
While 360 is still new Howell Marketing outperforms the rest. If you have any questions about 360 feel free to ask us! | 2019-04-24T10:00:01Z | https://www.howell.marketing/blog/360-marketing |
Our cucumber powder is made from premium cucumbers. Each product is thoroughly washed, trimmed, cut, and selected. The raw materials are dried by controlled, moderately heated forced air. After dehydration the cucumbers are sorted,sifted and screened to correct size. The end product is a fresh and tasty cucumber powder that can be uses for soups, dressings, dips, or any recipe you have that needs that cucumber flavor. | 2019-04-24T00:51:12Z | https://www.americanspice.com/cucumber-powder/ |
Sunday, Father’s day, is the open house for our home. We’ll be hiding out at church and at Kings Dominion all day so that we are not in the way.
So today, its cleaning out the garage, the packing room, and putting more touches of cleanliness on the house. Selling a house while trying to pack it up seems like a bad mix. Have to keep it clean and spotless, yet your trashing stuff, half used packing boxes, overflowing recylcing containers with papers from high school, selling stuff on craigslist and freecycling stuff — well, the house just doesn’t stay clean.
At church on Sunday, we’ll be presenting our minute for mission and saying our goodbyes. And our little Spanish church is planning on throwing us a big party in July.
With regards to our business, our houses have three showings this weekend that are currently scheduled.
Today: 33 days to departure. | 2019-04-19T13:17:52Z | https://www.missiontotheamericas.org/page/52/ |
North Carolina's Republican-controlled state legislature voted Monday night to override Democratic Gov. Bev Perdue's veto of a state budget that strips money from Planned Parenthood.
The same lawmakers overrode Perdue's veto and moved to defund Planned Parenthood last year, but a judge blocked the provision, arguing that a state can't single out a particular health provider. This time around, lawmakers found a way to sidestep legal challenges by not specifically naming Planned Parenthood in the budget. Instead, the bill prevents the state's Health and Human Services department from contracting with "private providers" of family planning services -- effectively, but not explicitly, singling out Planned Parenthood.
Planned Parenthood of Central North Carolina, one of the state's two Planned Parenthood providers, estimates that it will lose $200,000 as a result of the new budget. Because no state money is used for abortion services, the funding cuts will affect the clinic's ability to provide affordable birth control, cancer screenings, pap smears and other services for low-income women in the state.
"If we aren't able to figure something out and replace this funding, this will affect the price and availability of services and prescription drugs for patients," Paige Johnson, a spokesperson for PPCNC, told The Huffington Post. "We won't be able to provide the walk-in care we currently provide."
The new budget bill redirects $343,000 in state family planning funding from private family planning providers to county health departments, effective July 1, 2012, because private providers -- namely, Planned Parenthood -- also offer abortions.
Johnson said it was unclear whether the judge's 2011 injunction against the defunding provision would apply to the new budget, now that it doesn't specifically name Planned Parenthood.
"We're looking at all legal options," she said. | 2019-04-25T04:06:11Z | https://www.huffpost.com/entry/north-carolina-planned-parenthood_n_1646466 |
We have advice from Madrid, that the Points relating to Trade and Navigation, which are to be settled between the King of Spain and the States general of the United Provinces, will be referred to the Examination of Commissaries or Plenipotentiaries appointed by both Powers.
We hear that a certain Person having plung’d himself into great Difficulties, has call’d an extraordinary CONVENTION of his Friends, to beg their Assistance, on a remarkable Occasion.
We hear the King of Spain expects Satisfaction to be made him for beating and destroying the Spanish Armada in the GLORIOUS Reign of Queen Elizabeth, as well as for beating and destroying his Fleet in 1718, in the Mediterranean. | 2019-04-26T03:59:57Z | https://www.newsletter.co.uk/retro/reported-280-years-ago-march-20-1739-pleas-over-lost-locket-sleeve-button-set-in-gold-1-8860280 |
We spent the weekend doing mooncakes! Hahaha.
Anyway, for this week’s mooncakes, we are happy! Our first time doing the “traditional” snowskin mooncakes, and I think they look pretty good, which exceeded my own expectation.
Love the Hello Kitty ones!
The mooncakes are filled with coffee lotus paste – which is not really that sweet, and I like that 🙂 Some of the fillings had peach vodka white chocolate truffle centers, some were filled with semi sweet chocolate bits.
1st Step: Prepare the lotus paste by rolling into balls. Put them into the fridge before use to firm them up. Flatted the lotus paste, before we put in the chocolate truffles or chocolate bits.
2nd Step: Prepare the snowskin dough. Put the dough into the fridge for15 minutes before starting to work on the mooncakes.
3rd Step: Flatten and roll out the mooncake dough. Place the lotus paste inside the dough and roll them into a ball. Place dough into the mold and press.
Tadah! Mr Pau’s first try.
I like that I made the skin so thin! | 2019-04-22T06:52:27Z | https://cherienoms.com/2012/09/snowskin-mooncake-making.html |
Do you have trouble in completely uninstalling Winuptime 1.1 from your system? Are you searching for an effective solution to thoroughly get rid of it off your PC? Don't worry! You have come to the right place and you will be able to uninstall Winuptime 1.1 without any difficulty.
* Winuptime 1.1 does not work as good as you thought.
* You can not uninstall Winuptime 1.1 with Add or Remove Programs in Control Panel.
* You keep receiving error messages when you are uninstalling Winuptime 1.1.
* Winuptime 1.1 is still running in the background even after you uninstalled it.
* You can still find the folders and files of Winuptime 1.1 after the uninstallation.
There may be many other problems computer users run into when uninstalling Winuptime 1.1. As a matter of fact, those issues are caused by improper uninstallation of the program. Also, if the program is corrupted or damaged, users may not be able to uninstall it. Here, you will learn how to correctly and completely remove Winuptime 1.1.
1.Exit Winuptime 1.1 and end all of its processes before uninstalling it.
4.Find Winuptime 1.1 in the list and click Remove button to initiate the uninstallation.
If you do not want to use Control Panel to uninstall Winuptime 1.1, you can also uninstall it with its own uninstaller.
1.Go to the desktop and find the icon of Winuptime 1.1.
Both of the above uninstallation methods are the most common methods for people to uninstall Winuptime 1.1. Yet, they only allow you to uninstall small programs with few files and are not able to completely remove stubborn software like Winuptime 1.1. So, the possibility is that many invalid registry files and program files are left in your system and hard disk. If you leave them in your system, your system registry will be stuffed with useless entries and system becomes unstable and slow. In such situation, you need to manually delete those entries and files left in the registry and the system.
Step 3: Select Winuptime 1.1 and click Uninstall button to uninstall.
Download Automatic Winuptime 1.1 Uninstaller Now! | 2019-04-24T17:55:18Z | http://www.quicklyuninstall.com/article/Quickly-Uinstall-Winuptime-1.1.html |
Advertising is a form of marketing to promote a product, service of idea. Companies spend lots of money on advertising to promote their products and services and in the end hope that the advertising will translate to awareness and profits.
There are many ways of advertising such as television, radio, emails, newspapers to the rising advertising darling which is social media. The main aim of advertising is to reach as big an audience as possible.
Television is one of the most expensive ways of advertising as companies may choose to run an advertisement to brand their product or services or even sponsor a drama to get viewers interested in the product by inserting the advertised product in the drama. Mostly big companies with great cash backing will choose this to advertise their product or services. Though this is an expensive method, it is almost foolproof as we see how sales of a certain product soared when the actors in a popular drama used it during their shoot.
Newspapers used to be one of the popular advertising methods as almost everyone reads the local major newspaper so advertising in it guarantees that your product or services will be seen and known by the readers. It still is one of the core methods for some industries even though the paper circulation is facing a decline no thanks to the internet.
Some of us are used to getting catalogues or brochures in our mail. This is also a form of advertising and regular mailers may translate into sales in the long run. There is a limit to the number of customers who walk into your store. However, when you choose to mail out to your entire community you are almost sure that a percentage will look at the various services or products you can provide.
Another popular advertising method these days is using the internet. Some companies have started to invest in autoresponders which regularly send emails of interesting promotions to their subscribers on their mailing list. Others have engaged social media such as Facebook or Youtube to blast to subscribers what they are providing. This is a relatively inexpensive method and the reach is massive. If your online advertisement is entertaining or interesting, it can turn viral where customers share the ad online to their friends and you have suddenly a large group of followers wanting your product.
Companies may choose to employ a mixture of ways to advertise themselves based on their budget and target customer group. | 2019-04-23T02:17:06Z | http://www.marketingsingapore.com/advertising-for-local-businesses/ |
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A good manual! fast service and good qualityi for pdf document. thanks!
Very helpful and complete manual. Maybe only one negative is schematics have sometimes unreadable name of the parts. But it's not a big problem.
Excellent high quality schematics brought my old Heidelberg back to life. Fast download at a reasonable price. Thanks.
This document is just what I was looking for, it´s very useful, it contains adjustment procedures for the final stage of the power amp and also has a complete wiring diagram and description of the main semiconductors used in the design. | 2019-04-19T22:42:33Z | https://www.owner-manuals.com/505-service-manual-FISHER.html |
The death has taken place at the Carndonagh Community Hospital of Ellen Mc Gonagle, Upper Drung, Quigley's Point.
Funeral leaving the Carndonagh Community Hospital Chapel of Rest on Monday evening April, 8 at 6pm going to St Columba's Church, Drung, Quigley's Point to repose.
Requiem mass on Wednesday, April 10 at 11am with interment afterwards in adjoining cemetery.
Tommy's remains will repose at his late home from 5pm on Monday, April 8.
Funeral from his late residence on Wednesday, April 10 at 10.30am for Requiem Mass at 11am in St Patrick's Church, Crossroads, Killygordon. Interment afterwards in the adjoining Churchyard.
The death has taken place of Thomas (Tom) Calpin, Killbarron, Rossnowlagh Road, Ballyshannon, F94 E608.
Reposing at the family home Monday from 2pm until 10pm. Requiem Mass on Tuesday in Saint Patrick's Church, Ballyshannon, at 11am, followed by interment in The Abbey Assaroe Cemetery.
Enquiries to Patsy McCauley Funeral Director on 07703210437.
The death has occurred at Belfast City Hospital of Bernadette O’Hare, Donegal Road, Ballybofey.
Former Director of Nursing, at the Sean O’Hare Unit, Stranorlar. Her remains will repose at her late residence today Saturday from 3pm.
Funeral leaving her home on Monday at 10.30am for Requiem Mass in St Mary's Church, Stranorlar at 11am.
Interment afterwards in Antrim Cemetery, Moylena Road, County Antrim, BT41 4PA.
Family time please, from 11pm until 11am and on the morning of the funeral.
The death has taken place at the Donegal Hospice of Madge McKinney, Harbour View, Poundtown, Greencastle.
Madge's remains will repose at her home from 6pm this evening, Saturday.
Funeral from there on Monday at 10.15am for 11am Requiem Mass in St Pius X Church, Moville followed by burial in Ballybrack cemetery.
Donations in lieu of flowers to the Donegal Hospice c/o Liam Collins, Funeral Director or any family member. | 2019-04-23T18:56:04Z | https://www.donegaldemocrat.ie/news/deaths/374599/deaths-in-donegal-monday-morning-april-8-2019.html |
Olly upended the vitamin category by color-coding benefits, creating easy-to-understand variants and offering a fun chewable format.
The term “challenger” was first coined by Adam Morgan, author of “Eating the Big Fish: How Challenger Brands Can Compete against Brand Leaders,”* to describe brands that upend assumptions in a given category or industry. Today, beauty is in the age of the challenger brand—companies that disrupt the dynamics of segments, or even entire industries, and unseat the market leader.
Every category has a Goliath, a leader or dominant brand with the biggest market share that establishes the segment’s rules and conventions. Challengers play the David to their category’s Goliath. But being a small brand, or the number two or three player in a category, doesn’t automatically make a company a challenger.
What defines the challenger is a mindset. Challengers define what they are challenging, so that they can introduce new criteria of choice. They also typically have big ambitions that outstrip their resources. As a result, they must think smarter and be more agile in order to challenge the category’s behemoth.
Challengers can’t compete with the big fish on their own terms, so they introduce something new—a different approach to positioning, product, packaging, distribution or communication—thereby changing the rules of the game.
Every industry is subject to disruption by challengers, including hospitality (Airbnb), livery (Uber), education (Coursera), financing (Kickstarter) and retail (Etsy). Larger CPG brands, in particular, are prone to challenger disruption as a result of consumers’ changing expectations (where agility is in inverse proportion to size) and fragmentation of channels that reduces barriers to entry for emerging brands.
Disruption of a category needn’t be seen as a negative thing, said Mia Davis, head of environment, health and safety for Beautycounter, a California-based beauty and personal care company with an uncompromising focus on ingredient safety and transparency. Indeed, Beautycounter, which sells its products online, as well as via beauty consultants, sees itself as disrupting the entire beauty industry, with its rigorous ingredient selection process and explicit “Never List” (see photo) of more than 1,500 ingredients that it bans from its products.
Challengers live by several core principals. Here are just a few examples of how today’s beauty and personal care brands are challenging the Goliaths of their category.
Challenger brands find real opportunities for growth by viewing a category with fresh, or “innocent,” eyes. They ask simple, but intelligent questions that brands within the category may lack perspective to do. This “intelligent naiveté” is often a real advantage to a challenger brand, Morgan wrote in “Eat Big Fish,” because it takes big ambitions to change the way the world feels about a given industry.
When Craig Dubitsky, founder and CEO of Hello Products—the world’s first “naturally friendly” oral care brand—first surveyed the category, he was surprised by what he saw.
Dubitsky saw an opportunity to bring a friendly, human touch to the combative narrative of oral care.
“I wanted to elevate the everyday, turn a commodity into a desirable,” he said.
Hello Products achieved this transformation through vibrant packaging, appealing flavor combinations (including Mojito Mint and Blue Raspberry) and an eminently likable brand tone.
Olly, a vitamin and supplement brand, jumped from the health aisle into other areas, according to CEO and co-founder Brad Harrington. The brand also focused on the consumer experience of taking supplements.
In addition, Harrington and his team saw the vitamin category as being divided into two uninspiring factions: scientific-sounding brands such as Centrum and folksy brands such as Nature’s Garden.
Challenger brands are rigorous in identifying who they are and what they stand for and develop a very focused “lighthouse identity” based on an “inarguable brand truth.” Challengers project this identity intensely and consistently.
Brands with fewer resources need to know what they are not going to do, which can be as critically important as what they are going to do. It is not a question of prioritization, but rather eliminating nice-to-have, as opposed to must-have, targets. Indeed, this concentrated focus can be seen as one of the few advantages that challenger brands have in their arsenal, compared to market leaders.
This meant sacrificing how quickly the brand grew in the first year, including international expansion, in order to stay focused on product, product delivery and merchandising.
The flip side of sacrifice is over-committing, driving success through a relentless drive to succeed in one or two key activities.
Hello Products describes itself as being an intense listener.
Dubitsky makes himself easily accessible by Skype to anyone who contacts him, including dentists, dental hygienists, entrepreneurs, people living in China looking for Hello Products and even dads helping their kids on their school projects.
In short, brands can never obsess too much over their area of expertise. | 2019-04-23T14:57:45Z | https://www.gcimagazine.com/business/marketers/positioning/How-to-Be-a-David-396223361.html |
Total Mixed Ration or Pasture feeding? Deciding on the best method of cattle feeding will have long-term effects on your farm’s profitability. It’s important to go with the most effective option for your farm early. Two of the most prominent feeding systems are the Total Mixed Ration (TMR) and pasture, which have many differences. Here’s a brief outline of the two methods and some factors to consider.
What is a Total Mixed Ration (TMR)?
A Total Mixed Ration is a cattle feeding method that combines forages, grains, protein feeds, minerals, vitamins and feed additives. These ingredients are formulated to a specific nutrient concentration, all in a single feed mix.
TMR allows for improved feeding efficiency because each mouthful contains balanced ingredients.
The balance results in a stable environment for rumen microbes, offering the right amount of carbohydrates and nitrogen needed for digestion.
There are different rates of breakdown because of the various feeds, allowing for better nutrient use.
Forage is fed with concentrate rather than separately, this to reduce the chance of rumen digestive problems.
There’s a greater variety of by-product feeds, which allows for ration cost savings.
A decrease in digestive and metabolic problems.
An increase of up to 5% in milk production compared to conventional rations.
Provides greater accuracy in the formulation and feeding when well managed.
TMR is well adapted to mechanisation with a mixer wagon or stationary mixer with conveyors or mobile feeders.
Commodity ingredients, which are often less expensive in bulk order can be fed in a TMR diet.
Flavours of less palatable feeds are masked.
The TMR mixing devices needed for the ration will include costs related to equipment and maintenance.
Over mixing could cause grinding and pulverizing the feed.
Under mixing can result in a less effective feed for cattle.
Accurate weighing – using particular calibrated scale is essential.
An imbalance in the diet may result in reduced performance by the cattle.
Some housing and feeding facilities are not well suited to the TMR system.
It might not be economical for smaller herds to use TMR feeding.
Pasture-raised cattle receive a large portion of their nutritional intake from organically-managed pasture and stored-dried forages. They may also receive supplemental organic grains, both during the grazing season into winter months, which can include corn, soy, oats, barley, and triticale, among others. The cattle might also receive mineral supplements.
Pastured animals provide humans with more health-enhancing, disease-fighting materials than industrial-grade concentrated animal feeding operations (CAFOs).
Grass is the cheapest feed available so systems incorporating pasture are less costly.
Because of the lower cost, there is typically a higher profitability when compared with a similar herd size in confined systems.
There is increased health for the cattle – fewer instances of lameness or mastitis.
There is increased performance and fertility.
There is improved milk composition.
There are fewer environmental impacts.
There is a possibility of erosion.
There are high costs involved.
It’s important to assess various elements such as herd size, location, weather and costs before deciding on the best feeding method. There is also the possibility of implementing a ‘partial’ TMR, which could prove more beneficial for the herd, rather than opting for just one method.
For more information on feeding, pasture and tillage equipment, contact Radium Engineering. With years of expertise, a passion for the agricultural industry and a commitment to manufacturing superior equipment, Radium Engineering is the answer to all your farming and agricultural and tillage equipment needs. | 2019-04-22T04:06:31Z | https://www.radium.co.za/total-mixed-ration-vs-pasture/ |
Alexander Wurz is a former racing driver from Austria who has competed in various series, including Formula 1 and World Endurance Championship. Most recenty, after few years out of racing, he reactivated himself in rallycross.
Wurz recorded 69 starts in the Formula One World Championship between 1997 and 2007, driving for three teams (Benetton, McLaren and Williams). He scored three F1 podiums.
In sports car racing, he won Le Mans 24 Hours two times, in 1996 with Joest Racing and in 2009 with Team Peugeot Total. After four FIA WEC seasons with Toyota, Wurz retired as a driver at the end of 2015 but stayed to work for Toyota.
He was born on February 1974 15, in Waidhofen an der Thaya, in the district of Lower Austria. Interestingly, his first sport was cycling. He has competed in the BMX World Championship and in 1986 became a champion. He stayed involved in cycling even after he was established driver, formed his own team and even presented a limited edition of the high-quality mountain bikes.
Wurz’s motorsport career started relatively late considering that he is the son of a former rally driver and three-time European champion, Franz Wurz. He was driving karting and in 1991 moved to German Formula Ford while two years later he was in the German Formula 3 Championship.
In the following year, Alexander became the series runner-up what was his best achievement until 1996. That year Wurz won 24 Hours of Le Mans for the first time. Driving Joest Racing’s TWR Porsche WSC-95 alongside Davy Jones and Manuel Reuter he surprised many and become the youngest ever winner of the prestigious race, at the age 22.
The same year, driving Joest’s Opel Calibra, Wurz spent a full season in the International Touring Car Championship and did relatively well, scoring points in the most of the races but he couldn’t score any top 5 finish.
In 1997 Wurz has competed in the FIA GT Championship driving a Mercedes. He won one out of 10 races and finished 10th in the championship. However, the most important thing that year was unexpected Formula 1 debut.
He jumped into the seat of Benetton, replacing Gerhard Berger in the Canadian Grand Prix. He retired from the race, just as in the French Grand Prix, but impressed in his third and last appearing that year, finishing 3rd in the British Grand Prix, behind Jacques Villeneuve and Jean Alesi.
That result persuaded everybody in Benetton to promote Wurz to a race driver in 1998. The first half of the campaign was really good for the Austrian who finished in Top 5 in six out of nine races, but later his performances deteriorated and in the last seven races he couldn’t win a point. Anyway, he was 8th in the Drivers’ Championship what was a result worthy of respect for the rookie.
Wurz stayed with Benetton for another two seasons but the results weren’t any near to the ones from the previous one. In 1999 Alex scored only in two races, in Monaco and Austria, while in 2000 he won points only in the Italian Grand Prix. He left the team at the end of the season but stayed in Formula 1 World championship.
In 2001 Wurz signed with McLaren F1 team but acted only as the test driver for five years! His only chance to race in Formula 1 appeared in 2005 when he replaced Juan Pablo Montoya in San Marino Grand Prix and finished 17th in the race. He left McLaren at the end of 2005 to join Williams F1 team but again only as the reserve and test driver during 2006.
Williams promoted Wurz to a race driver in 2007 but he struggled during most of the season. However, there were some bright moments for Alex like 3rd place in the Canadian Grand Prix and 4th in European Grand Prix. With 13 points on his account, Wurz finished 11th in the final standings and decided to leave the series, saying that he was unable to fully commit to his duties and added that he will turn to endurance racing and road safety.
He stayed involved in Formula 1 as a test driver for Honda and Brawn GP in 2008 and 2009 while in 2012 acted as a tutor to Williams’ young drivers Bruno Senna and Pastor Maldonado.
After many years, Wurz returned to Circuit de la Sarthe in 2008 with Team Peugeot Total to finish fifth at 24-hour race. The following season he scored his second victory there, driving Peugeot 908 HDI FAP alongside Marc Gene and David Brabham. That was his last triumph at Le Mans but in the following years, Wurz scored some notable results, driving for Peugeot and Toyota factory teams.
Since 2012 Alexander Wurz has a contract with Toyota Racing to drive one of their cars in the FIA World Endurance Championship. In his first season in the WEC Wurz scored three wins together with Nicolas Lapierre and Kazuki Nakajima to finish 3rd in the overall standings. In the following two years, he finished 4th and 5th while in 2015 Wurz was 6th, failing to win a single race for the first time.
In a meantime, Wurz has won many famous endurance races, like 1000 km of Spa, 12 hours of Sebring or Petit Le Mans at Road Atlanta, establishing himself as one of the most accomplished drivers in the last decade.
Nowadays Wurz is one of the most influential and most respected people among the drivers. He is involved in many projects of drivers educations and training, as well in the road safety programs. His academy is closely connected with the FIA and supports various programs intended to young drivers. Wurz was also the president of Grand Prix Drivers’ Association.
Most recently, he was involved into the selection process for the brand new all-female W Series.
After leaving Toyota's FIA WEC programme at the end of 2015, Wurz continued to work for Toyota. In January 2016, he joined Chip Ganassi Racing to drive Riley-Ford prototype at 24 Hours of Daytona, finishing in the fifth place.
After that, he was out from competitive racing for several years, reactivating himself in 2018 as a rallycross driver. He joined compatriot Max Pucher and competed with his team in two rounds of the World Rallycross Championship. For 2019, Wurz was announced as one of drivers in brand-new GRC Europe rallycross series, organized by Max Pucher. | 2019-04-26T10:14:40Z | http://www.snaplap.net/driver/alexander-wurz/ |
Can (and should) the US back the Dollar with gold??
”Over the weekend I’ve been mulling over the US’s problem of its falling dollar (of course the US may want a weaker dollar in order to pay off its debts). If I was Secretary of the Treasury, I would ask myself, ‘What could I do to render the dollar more attractive?’ Here’s an idea that occurred to me, and this is what I might do.
The US owns the largest hoard of gold on the planet (in terms of sheer number of ounces). Suppose the US decided to back the ‘dollar’ with gold. That would immediately make the dollar more attractive to dollar-holders. But if the US decided to back the dollar with gold, skeptical investors, worldwide would be likely to turn in all their dollars to the US and soon clean out the US’s hoard of gold.
The dollar would be backed by gold, making it a one-of-a-kind currency, but few people would be willing to turn in their dollars for ‘expensive’ US gold.
Nevertheless, the dollar would be the only gold-backed currency on the planet. And that would render the dollar a wanted currency, perhaps the world’s most wanted currency, because it would be the only currency backed by something tangible.
But wait, there’s a problem. If it requires $5,000 dollars to buy a single ounce of gold, isn’t the dollar being effectively devalued in that it would require an enormous amount of dollars to buy an ounce of gold?
So the key question — If the US unilaterally raised the price of gold, would it be inflationary or deflationary? Do any of my subscribers have an answer?
In actual practice, the dollar price of gold has been rising for 10 years in succession. Each year it requires more dollars to buy one ounce of gold. Thus, against gold, the dollar has been declining for years. This means that the dollar, in terms of gold, is being devalued by the market.
Gold is traded around the world, 24 hours a day. Thus, it cannot be manipulated by the US or any single country. Gold’s price is a function of what people around the world will pay for it at any given time. If people believe their currency is declining, they will be willing to put up more of their own currency for an ounce of gold. | 2019-04-19T01:07:51Z | https://www.monex.com/value-ataglance/can-and-should-the-us-back-the-dollar-with-gold/ |
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Egypt Jaw Stone Crusher Supplier. What is price of a 10 TPH small diesel stone crusher for sale in a . Small diesel stone crusher and mobile crushing plant are technical revolution . Coal Fired Power Improve Productivity & Safety Martin Engineering . Nigeria Used Coal Crusher Power Plant For Barite,Dolomite Buy . | 2019-04-20T00:34:37Z | http://theaceaward.org/leadmc/14970/7229/safe-and-durable-crushing-plant-for-barite-in-egypt/ |
Yesterday had been a long day and my brain had turned to mush so when looking at my blank sketch pad on the way home on the train yesterday the first thing that came in to my mind was this.
Blame the tiredness! And the long day! And missing me train! And the heat! Please? | 2019-04-18T23:05:51Z | https://lloydandthebear.com/?p=560 |
B. Albrecht hit by pitch.
T. Luckey hit by pitch; B. Albrecht advanced to second.
M. Plato doubled, RBI; T. Luckey advanced to third; B. Albrecht scored.
M. Plato advanced to third.
R. Highden singled; B. Wrolstad advanced to second.
J. Factora struck out swinging, reached first on an error by c.
A. Oasay reached first on an error by 3b.
B. Wrolstad walked; A. Oasay advanced to second.
M. Plato singled, advanced to second on an error by lf.
C. Santillan singled; M. Plato advanced to third.
T. Toepke singled, RBI; C. Santillan advanced to second; M. Plato scored.
C. Santillan advanced to third.
C. Benson to p for T. Fox.
N. Soltero to p for R. Shipley.
C. Russell out at first ss to 2b, RBI; N. Paragoso scored.
A. Oasay hit by pitch; C. Isa advanced to second.
K. Cook to p for C. Benson.
S. Fagerness pinch hit for N. Ka'ahanui.
T. Henry pinch hit for T. McGrath.
D. Miller pinch hit for T. Henry.
C. Tam pinch hit for J. Hinkle. | 2019-04-26T02:30:47Z | http://bbccvikings.com/sports/bsb/2018-19/boxscores/20190317_dqfr.xml |
Sydney Metro is the biggest urban rail project in Australian history, transforming how we get around the nation’s biggest city.
The Sydney Metro West project is Sydney’s next big railway infrastructure investment.
It will deliver a direct connection between the CBDs of Parramatta and Sydney, linking communities along the way that have not been previously serviced by rail and unlocking housing supply and employment growth between the two major CBDs.
This means Sydney Trains and inter-city services will be freed up for customers in the city’s outer west in areas like Blacktown, Penrith and the Blue Mountains. In turn, these services will become more reliable, with increased capacity, reduced crowding and faster travel times.
As a new railway, Sydney Metro West will work together with the existing T1 Western Line, effectively doubling rail capacity from Parramatta to the CBD and supporting the Greater Sydney Commission’s vision for better connections between these two major centres.
The NSW Government will integrate transport and land use planning along the corridor, with the project will be delivered as an underground metro railway line.
Community, stakeholder and industry consultation will now help shape Sydney Metro West’s development, including the alignment and potential station locations.
The Sydney CBD, allowing easy access to the existing public transport network and Stages 1 and 2 of Sydney Metro, which is currently under construction.
Find out more on the Sydney Metro West website. | 2019-04-26T14:24:34Z | https://www.transport.nsw.gov.au/projects/current-projects/sydney-metro-west |
It is fairly satisfying to create works of artwork. From scrapbooking, developing models to making jewelry, there is certainly a craft action that will match any a single of your passions. Hold reading through to find out more about the matter.
Go simple on youngsters who are doing arts and crafts. You need to bear in mind that this is developed to foster their creativeness. If you are pointing out imperfections, then their self self-confidence will experience.
Maintain your provides for arts and crafts well arranged. Your provides can be saved in numerous numerous techniques, just locate a single that works the very best for you. When provides are arranged, you can simply locate what is essential for a project. Additionally, firm aids make sure you have what you want to total your crafting venture.
Numerous little ones take pleasure in arts and crafts. Try to consider of initiatives your young children will take pleasure in doing work on. If you never know what to do, inquire a good friend or seem online.
If you are starting out early on in oil portray and discover your latest canvas creation isn’t all that great, just paint anything new on top of it. Oil is adaptable, and the area will not be harmed by extra coats. It can make your creation have a exclusive layout and depth.
Make a chicken feeder with peanut putter and a pine cone. Merely smear peanut butter on the outside the house of the pine cone. Roll the sticky pinecone in birdseed. The seeds will stick to the cone even following the peanut butter dries, so use a string to hold it from and tree and look at as the birds feast on it.
There is so a lot to find out about arts and crafts, and this report is a great beginning level. Your hobby dreams can appear true when you put this information to use. With the aid previously mentioned, you are now ready to make gorgeous items to take pleasure in for years to come. | 2019-04-23T03:05:41Z | http://www.google-logos.com/tag/google-doodles |
Many people think that the key to success is to cultivate and doggedly maintain an optimistic outlook.
What about humans? Human groups are not like ant colonies.
Children who play the violin or study piano could be learning more than just Mozart. | 2019-04-21T21:03:40Z | https://daily-archive.jackwestin.com/mcat-cars-practice/category/psychology/ |
And if that wasn’t exciting enough, we are also announcing a store expansion. Now you’ll be able to shop for even more fresh summer stock.
See you on Saturday, London!
P.S. There’s also going to be birthday treats (happy birthday Nat!) and cold brew. | 2019-04-24T09:51:54Z | https://filthyrebena.com/blogs/news/flash-sale |
Our new website will be online very soon !!!
Enjoy and discover the Restaurant Le pressoir in Grand Case, Saint Martin !!!! | 2019-04-20T15:13:52Z | http://www.lepressoirsxm.com/news/new-website/ |
I have studied the Palmarian Church for many years. In May 2017, I published a book on the subject and through the years I have written some twenty blog posts on El Palmar de Troya and the church’s development, structure and teaching. Interestingly enough, the Palmarian Church plays a part in bestselling author Dan Brown’s most recent book, Origin, which was published in September 2017.
Why haven’t I mentioned Origin in any blog post until now? The reason is simple. I think that it is a very bad book even by Dan Brown standards. In the afterword, the author, as always, claims that the book is based on much research. I doubt it and I wonder how it is possible to write such a boring book about such an intriguing theme as the Palmarian Church. Only Dan Brown knows and does he even care?
If you want to read something good about Brown and El Palmar, I would suggest that you read Damian Thomson’s “Dan Brown’s new target?: The creepy Catholic sect with its very own pope and ‘Vatican'”, published in The Spectator in October 2017 or why not Matthew Walther’s “Dan Brown is a very bad author”, published in The Week at about the same time. | 2019-04-18T12:56:46Z | https://magnuslundberg.net/category/dan-brown/ |
Alex Carrigan is a recent college grad hailing from Newport News, Virginia. Growing up, Alex was very much drawn to media, spending a lot of time reading books and watching television and movies. As he got older, Alex began to spend more time researching media and learning more about the various books, shows, and films he was exposed to. In high school, Alex began to take a turn more towards his future goals. He took classes on studying film and creative writing and became more interested in the fields. He was accepted to Virginia Commonwealth University in 2010 and moved to Richmond, Virginia to attend the school.
At VCU, Alex received a major in print/online journalism and a minor in world cinema. While his interests and career plans changed radically in college, Alex did discover that he wants to live a life engaged in artistic passions, where he could meet and engage with creative individuals and write about them. It was in this time that Alex decided to try and merging his interests, using his degrees in order to try and work for an arts publication or publishing group.
Alex first got a taste for the literary scene when he joined a literary journal called Poictesme in 2010. In Poictesme, Alex got to read poetry and prose submitted by students, critique them, then help create an annual publication showing the best of VCU student art and literature. He stuck with the group all through his college career, even obtaining the rank of deputy editor-in-chief from 2012-2014. Because of changes within Poictesme, Alex and the editor got to go to AWP 2014 in Seattle in order to promote Poictesme, attend panels, and prowl the book fair. It was at the book fair that Alex met Cambridge Writers Workshop and passed along his resume. The rest is history.
Alex is excited to be the new Editorial and PR intern for Cambridge. He hopes to help promote the group online and assist in the various projects of the group. He can also be followed on his blog at carriganak.wordpress.com. He also can be followed on Twitter @carriganak and his Facebook page “Alex K. Carrigan, Journalist.” He is also the staff film reviewer for Quail Bell Magazine and has articles published regularly there.
This entry was tagged intern. Bookmark the permalink. | 2019-04-22T16:40:19Z | https://cambridgewritersworkshop.org/2014/06/24/meet-alex-carrigan-our-new-editorial-and-public-relations-intern/ |
Financial contributions from individuals, businesses, civic groups and foundations are vital to each resident's ability to live independently with grace, comfort and dignity. Your tax-deductible, financial contribution – however great or small – is greatly appreciated and helps our community move forward day to day, month to month, year in and year out. Thank you for your generosity!
Your Household Gifts Turn Into "Miracles"
Our low-income residents are sometimes forced to choose between purchasing food or purchasing medications. By donating perishable food items, you are helping supplement the nutritional needs of those who need it most.
Please click here for a complete list of requested food items.
Please call us at 520-624-5860 or send an email using the form below if you would like to donate to Miracle Square, volunteer your time or services, or if you have any general questions. | 2019-04-20T13:19:42Z | http://www.miraclesquare.org/contact |
From Franklin Square you are about 16 minutes away. Take New Hyde Park road north to Jericho Turnpike (Jamaica Avenue) Route 25. Make a right and head east about three miles to Willis Avenue. When you get to Willis, make a left and head north. About 1 mile up Willis Avenue is our location. Destination will be on the left side of the street.
Listed below are actual vehicle purchases from sellers in Franklin Square over the years. | 2019-04-23T02:45:16Z | https://www.sellyourcarforcash.com/towns/franklin-square.php |
Exclusive EMI options of 3, 6 II 9 months.
Minimum cart value for EMI transactions are Rs.2500 for ICICI and HDFC bank Credits and Virtual Cards.Rs.5000 for SnapMint EMI loan (No Credit Card required).
Exclusive EMI options of 4, 7 II 9 months. | 2019-04-22T12:02:17Z | https://www.vijaysales.com/jbl%20t450%20headphone%20(blue)/6299 |
The Ranger is distributed in North America by Synergy Systems, Inc. Synergy Systems was given the opportunity from Outram Research Ltd in 2003 to be in charge of sales, marketing, and support in North America. Synergy Systems origin dates back to the Ranger 1 and they recruited individuals that had a long Ranger history to help with that support. Today, if you call for support you will probably talk with someone with over 20 years of experience with the Ranger line.
Ranger data loggers began life in Southern England in 1984. A small, handheld, 4 channel, data logger, was licensed to Gulton Graphic Instruments Inc of Rhode Island, owners of the Rustrak paper chart recorders.
The name Ranger (from the much loved character, The Lone Ranger) was chosen for the logger and the accompanying software was called Pronto (nearly the same as Tonto, the Lone Ranger’s trusty Indian companion). Early advertisements encouraged customers to buy by declaring, “save a lot of silver too.” (The Lone Ranger’s horse was Silver).
The original (Rustrak) Ranger (RR400) with a whooping 8kb of memory was one of the first electronic, graphing, logging devices and replaced the paper chart recorders which recorded temperature primarily, but also 4-20 mA and 0-2 Volts. Much thought went into the design of the user interface, culminating in a very simple, two button, selection operation. The DOS based software Pronto managed the data and was appreciated by customers for its ease of use, clarity and reliability. (and it could be ran without a hard drive which was important in those days).
After Ranger 1 came Ranger II, retaining the same essence as this first Ranger. In addition it had more channels and was very versatile, offering a range of pods for different applications. Gradually power quality measurement capability appeared and indeed started to take over. The Ranger II series measured power parameters. It soon became clear that this was a market on which to concentrate as it requires a high level of expertise.
The Ranger technical team thrive on difficult challenges. The first instrument dedicated to power quality measurement was the Power Logger, then came the Harmonic Analyzer the HA5000 and finally, still under license, the PM6000. Adaptive Store delighted customers with the increasing detail it provided as its techniques and the instruments evolved. Pronto became Pronto for Windows.
In 2003 Outram Research Ltd (ORL), the Ranger design house, negotiated back the sole rights to the Intellectual Property for the brand and is now able to focus fully on Ranger’s destiny. The culmination of its years of experience in the data logger market is a state of the art, extended family of Ranger Power Quality Analyzers. Each member of the family is described on this website.
ORL made the excellent decision of recruiting their most successful distributor to head sales, marketing, and support for the America's, Synergy Systems Inc. Synergy Systems origin dates back to the Ranger I and they recruited individuals that had a long Ranger history to help with that support. Today, if you call for support you will probably talk with someone with over 20 years of experience with the "Rustrak" Ranger line.
Today, Synergy Systems supports users around the clock with the top notch support you can only get from that amount of experience. Not only are customers supported daily from the USA, repairs are also performed by our experienced staff, here in the USA. | 2019-04-26T12:53:43Z | http://www.rangerpq.com/about-us.html |
Philadelphia, PA: Livent Corp. (NYSE:LTHM), producer of efficiency lithium compounds, launched its This autumn 2018 outcome which didn’t reside as much as analyst expectations, inflicting the stock to fall by 6.63% within the buying and selling session. While the corporate’s revenues are diversified throughout multiples geographies akin to North America, Latin America, Europe, Middle East and Africa, and Asia Pacific, its heavy dependence on Chinese consumption led to the corporate lacking out on income estimates.
Livent reported a top-line of $119.80 million which is a 5.6% development over the corresponding quarter of the earlier yr however it did not impress analysts who had anticipated the revenues to be $3.18 million greater. The reported EPS of $0.23 was in step with analyst expectations however this was not ok to forestall the stock from crashing.
In the phrases of the administration, the slowdown in China within the latter half of 2018 resulted within the firm’s Chinese prospects not being prepared to make firm commitments for value and quantity at ranges that had been acceptable to the group. As a outcome, the corporate has determined to focus its Asia-Pacific operations on economies like Japan and South Korea whereas hoping for the Chinese market to stay flat. To create optimism across the stock, the administration went forward and claimed that FMC Corp. is ready to spin off its stability stake in Livent on March 1, 2019 and that it remained optimistic concerning the elementary drivers of demand for its business. While the corporate focuses on battery-grade lithium hydroxide, butyllithium, and purity lithium metallic that are utilized in numerous efficiency purposes, the enterprise of lithium hydroxide is predicted to get a lift because of the robust rise in electrical car gross sales.
In phrases of outlook, CEO Paul Graves and his group count on Livent to generate a income of $495 million to $525 million for 2019, offering for a 12-19% development within the top-line. They count on the annual EBITDA to be within the vary of $190 million to $200 million and an EPS starting from $0.92 to $0.98 per diluted share.
While the 2019 outlook appears fairly optimistic, it’s value noting that the stock has already misplaced greater than 25% of its worth over the previous 3 months. It is to be seen if the corporate is ready to ship on the outlook that it has promised and if the administration can efficiently create shareholder worth in 2019. | 2019-04-19T16:26:47Z | https://smallcapsdaily.com/china-slowdown-revenue-miss-causes-a-drop-in-livent-share-price/ |
Очки Peltor MAXIM (зеркальные), 13227-00000М - очень легкие очки для использования с наушниками. Обеспечивают защиту глаз и спереди, и сбоку без ограничения обзора.
The 3M™ Maxim Spectacles has an aspherical lens for 180° of pure vision and superior eye coverage. Ventilation channels across the lens to minimise misting with a DX coating to resist fogging, scratching and chemicals. Soft, universal nosebridge with dual-injected cushion browguard for increased comfort and shock absorption. Adjustable temple lengths and pantoscopic (angle) adjustments. | 2019-04-21T14:30:36Z | http://forgun.ru/zaschita-sluha/ochki-peltor-maxim-zerkalnye-13227-00000m.html |
We arrived in Shroud Cay when the wind and the wave were not so friendly..we battled to tie up at the mooring ball. But did not stop us after the boat is tied, we jump at dinghy and went exploring the mangrove creek...., what we can say..that was incredible awesome! Take a look some of the pictures below..the pictures could not capture what we saw..From Shroud Cay we continued to Normans Cay and...to Highborne Cay..will updating soon the complete story.. | 2019-04-20T13:00:01Z | http://sabre425.blogspot.com/2011/04/exploring-mangroves-creek-shroud-cay.html |
"RSCA" redirects here. RSCA may also refer to Rotating Savings and Credit Association.
This article is about the men's football team. For the women's football team, see RSC Anderlecht (women). For the racing team, see R.S.C. Anderlecht (Superleague Formula team).
Royal Sporting Club Anderlecht, usually known as Anderlecht (Dutch pronunciation: [ˈɑndərlɛxt], French: [ɑ̃dəʁlɛkt], German: [ˈandɐlɛçt]) or RSCA (Dutch: [ˌɛrɛseːˈjaː, -seːˈʔaː], French: [ɛʁɛsse.ɑ], German: [ˌɛɐ̯ʔɛs.tseːˈʔaː]), is a Belgian professional football club based in Anderlecht, Brussels Capital-Region. Anderlecht plays in the Belgian First Division A and is the most successful Belgian football team in European competitions, with five trophies, as well as in the Belgian domestic league, with 34 championship wins. They have also won nine Belgian Cups and hold the record for most consecutive Belgian championship titles, winning five between the 1963–64 and 1967–68 seasons.
Anderlecht have been playing their matches in the Astrid Park in the municipality of Anderlecht since 1917. Their current stadium, Constant Vanden Stock Stadium, was first opened in 1983, and replaced the former Emile Versé Stadium. They play in purple and white outfits. They have long-standing rivalries with Club Brugge and Standard Liège.
Founded as Sporting Club Anderlechtois on 27 May 1908 by a dozen football lovers at the Concordia café (located in the Rue d'Aumale/Aumalestraat in the municipality of Anderlecht), the club beat Institut Saint-Georges in their first match, 11–8. They joined the official competition in 1909–10, starting at the lowest level in the Belgian football league system, then the third provincial division. In 1912–13, they gained promotion to the second-higher level of football, then named the Promotion. After only one season at that level, the championships were suspended due to World War I, and resumed in 1919–20. With the popularity of the team increasing, Anderlecht had moved to a new stadium in the Astrid Park in 1917 (then known as Meir Park). They baptized the stadium Stade Emile Versé in honor of the club's first major patron, the industrialist Emile Versé.
At the end of the 1920–21 season, Anderlecht were promoted to the first division for the first time in their history. In the next 14 seasons, Anderlecht were relegated four times (1923, 1926, 1928 and 1931) and promoted four times (1924, 1927, 1929, 1935), earning themselves the mockery of local rival clubs Union Saint-Gilloise and Daring Club de Bruxelles, who nicknamed them the "lift club". In 1933, 25 years after their formation, the club changed their name to Royal Sporting Club Anderlechtois. Since their promotion in 1935, Anderlecht has remained at the top level of football. With Jef Mermans, a striker signed from K Tubantia FC in 1942 for a record fee of 125,000 Belgian francs, Anderlecht won their first league title in 1947. Their success increased in the following years as they won six more titles between 1949–50 and 1955–56 (winning three consecutive titles twice) and two more in 1958–59 and 1961–62. In the 1960s, under the coaching of Pierre Sinibaldi and then of Andreas Beres, the club even won five titles in a row (from 1963–64 to 1967–68), which is still a Belgian league record. The star of this team was Paul Van Himst, topscorer in 1965, 1967 and 1969 and Belgian Golden Shoe winner in 1960, 1961, 1965 and 1974.
Anderlecht played in the first European Champion Clubs' Cup in 1955–56, and lost both legs of their tie against Vörös Lobogo. They had to wait until the 1962–63 season to win their first European tie, with a 1–0 victory over Real Madrid, which followed a 3–3 draw in Spain. For the first time, they advanced to the second round, where they beat CSKA Sofia before losing to Dundee in the quarter-finals. In the 1969–70 Inter-Cities Fairs Cup, Anderlecht lost in the final against Arsenal. Between 1975 and 1984, Anderlecht only won one championship but they achieved considerable European success: they won the 1975–76 and 1977–78 European Cup Winners' Cups against West Ham United and Austria Wien respectively, as well as the two subsequent European Super Cups.
The 1982–83 season was a noteworthy season for the club for numerous reasons: former Anderlecht favourite Paul Van Himst was named the new coach, they won the 1982–83 UEFA Cup and the rebuilding of the club stadium began. But in the domestic league, Anderlecht had to settle for second place behind Standard. Their bid to retain the UEFA Cup in 1983–84 failed at the final hurdle against English side Tottenham Hotspur. Anderlecht reached the final controversially by beating another English side, Nottingham Forest, with a debatable extra time penalty to win 3–2 on aggregate. It was later found Anderlecht had bribed the referee the equivalent of £27,000 to ensure passage to the final.
After three second-place finishes in a row, the Purple and Whites secured an easy 18th title in 1984–85, 11 points ahead of Club Brugge. In 1985–86, Anderlecht won the championship again, but this time after a two-legged play-off against Club Brugge. Anderlecht won their 20th championship on the last matchday of the 1986–87 season. They then lost key players Franky Vercauteren, Enzo Scifo (transferred in the summer of 1987) and Juan Lozano (heavily injured in a game at KSV Waregem a few months earlier). A weakened team coached by Raymond Goethals finished only fourth in 1988 behind Club Brugge, KV Mechelen and Royal Antwerp, but they nonetheless managed to lift the Belgian Cup for the sixth time in club history after a 2–0 victory over Standard Liège, with goals by Luc Nilis and Eddie Krnčević. The next year, Anderlecht retained the trophy with goals by Eddie Krncevic and Milan Janković (again with a 2–0 win over Standard), but finished second in the championship. After his second cup win, Goethals left for Bordeaux in the French Ligue 1.
During the 1990s, Anderlecht reached one more European final, the 1990 European Cup Winners' Cup Final, which they lost to Italian club Sampdoria. The club then declined in European competitions, with the 1990–91 and 1996–97 UEFA Cup quarter-finals their best results. In national competition, they won four championship titles and a cup. During the 2000s, Anderlecht secured five more Belgian champion titles, reaching a total of 29 titles in 2007, in addition to one more cup victory. In the 2000–01 UEFA Champions League, they qualified for the first time to the second round, then another group stage, where they finished third in their group behind Real Madrid and Leeds United.
In 2009–10, the Purple and Whites won their 30th Belgian league title, while in the 2011–12 UEFA Europa League, Anderlecht made history by becoming the first Belgian team to finish the group stage of a European competition with the maximum number of points, dominating group opposition Lokomotiv Moscow, Sturm Graz and AEK Athens. They were also the only team of that year's Europa League to achieve this feat. On 6 May 2012, Anderlecht won their 31st Belgian championship, while on 22 July, they won their tenth Belgian Super Cup.
Anderlecht colours are purple and white, and the club's home kit is generally white with purple trim, though they did wear a black and purple home kit in the 2005–06 season, and a grey in the 2007–08 season. In the beginning, purple was the main colour of the shirts. The motto of Anderlecht ("Mens sana in corpore sano") is written on its badge as are the three letters "SCA", referring to the initial name of the club (Sporting Club Anderlechtois). A crown was added in 1933 following the name change to Royal Sporting Club Anderlechtois. Anderlecht's colours inspired those of Al Ain FC in the United Arab Emirates.
Anderlecht play their home matches at the Constant Vanden Stock Stadium located within the Astrid Park in the municipality of Anderlecht. It currently has a capacity of 21,500 places. The works should start in 2011 and last two years. Anderlecht has been playing in the Astrid Park since the building of the Emile Versé Stadium in 1917. The stadium was completely rebuilt in 1983 and it was renamed in honour of the then chairman Constant Vanden Stock. Prior to 1917, the club has played on a ground in the current Rue du Serment/Eedstraat for a couple of years since 1908, then in a stadium located Rue Verheydenstraat (now Rue Démosthènestraat). In 2013 the stadium was refreshed, installing new scoreboards and advertising strips along the perimeter of the pitch in accordance with UEFA regulations for the Champions League. ColosseoEAS was chosen as the provider for the ultra-modern LED strips and their controllers.
Anderlecht were due to move to the 60,000 capacity Eurostadium upon its expected completion in 2019. The Eurostadium will also be home of the Belgium national team and host fixtures in UEFA Euro 2020. However, during the years that followed, the project was plagued by numerous political delays. In February 2017, Anderlecht eventually pulled out of the project.
The club has had the highest average attendance in the Belgian First Division for ten years, until 2004–05. Anderlecht supporters hail from all over the country and only a minority come from the Brussels Capital Region. Anderlecht counts 77 fan clubs, of which 5 are abroad (one in France, one in Poland, one in Texas, one in Montreal and one in Sunderland).
For a list of all former and current R.S.C. Anderlecht players with a Wikipedia article, see Category:R.S.C. Anderlecht players.
For a list of former and current managers, see List of R.S.C. Anderlecht managers.
There have been a total of 37 permanent managers and 3 caretaker managers of Anderlecht since the appointment of the first manager, Sylva Brébart, in 1920. The club's longest-serving manager is Englishman Bill Gormlie, who served during nine seasons between 1950 and 1959. Frenchman Georges Perino is the first Anderlecht manager to have claimed a trophy, with the first championship win in 1946–47. Seven Anderlecht managers have managed the club on two occasions: Ernest Churchill Smith, Pierre Sinibaldi, Urbain Braems, Raymond Goethals, Arie Haan, Johan Boskamp and Franky Vercauteren. Other managers have also played another role in the club before being appointed manager, including Jean Dockx, who served three times as caretaker before being appointed manager.
In 1993, Brussels D71 became Anderlecht's women team. The team has won three Leagues and five Cups since.
^ "Een nationaal stadion stamt uit de koloniale tijd". erasmix.be. 15 May 2013. Retrieved 23 November 2018.
^ "UEFA coefficient". Retrieved 28 August 2012.
^ "UEFA coefficient". Retrieved 1 November 2010.
^ "RSC Anderlecht official website". Archived from the original on 2 September 2010. Retrieved 24 October 2010.
^ "Forest sues Anderlecht over '84 bribery scandal". BBC News. 24 December 1997.
^ Gallez, Marcel & Serkijn Johan (2008). Le dictionnaire du RSC Anderlecht (tome 2). Magnad. p. 122. ISBN 978-2-9600723-3-4.
^ "Anderlecht crowned champions of Belgium By Berend Scholten". Berend Scholten on UEFA.com. 6 May 2012.
^ "Anderlecht edge Lokeren to lift Belgian Super Cup By Berend Scholten". Berend Scholten on UEFA.com. 22 July 2012.
^ "Histoire 1908–2005" (in French). Archived from the original on 2 September 2010. Retrieved 1 November 2010.
^ lej, mvd,. "Anderlecht speelt vanaf 2019 in nieuw stadion".
^ UEFA.com. "UEFA EURO 2020 hosts to be revealed in Geneva". UEFA.com.
^ "'Doodsteek dreigt voor Eurostadion'". De Tijd (in Dutch). 29 April 2016.
^ "En nóg een mokerslag voor Eurostadion: verkeerde rechter en opnieuw extra vertraging" (in Dutch). Voetbalkrant.com. 6 May 2016.
^ "Anderlecht stapt uit Eurostadion-project". Het Laatste Nieuws (in Dutch). 17 February 2017. | 2019-04-23T12:54:27Z | https://en.m.wikipedia.org/wiki/R.S.C._Anderlecht |
Trichophyton mentagrophytes is epidemiologically divided into two distinct forms, zoophilic and anthropophilic. The zoophilic isolates of T.mentagrophytes have generally been identified by morphological and biochemical examination as well as through mating experiments. The confirmed teleomorphs of the zoophilic isolates of the T.mentagrophytes complex are Arthroderma benhamiae, A. simii and A. vanbreuseghemii. On the other hand, no teleomorph has been identified in an anthropophilic isolate of T. mentagrophytes, such as T. mentagrophytes var. interdigitale (T. interdigitale) or in the other anthropophilic strains. In the present study, the mating type (MAT) (－)-specific gene of the MAT1-1 (alpha-box) and the MAT (＋)-specific gene of the MAT1-2 (high-mobility-group : HMG) DNA binding domain were confirmed in zoophilic dermatophytes of A. benhamiae, A. simii and A. vanbreuseghemii. The sequence of the MAT1-1 was about 1.3kbp, containing 2 exons in the A. benhamiae, A. simii and A. vanbreuseghemii (－) mating type strain. The sequence of the MAT1-2 was 1.9 kbp, containing 2 exons in the A. benhamiae, A. simii and A. vanbreuseghemii (＋) mating type strain. Of 15 animal isolates and 72 human isolates examined, the MAT1-1 was detected in 5 of the animal isolates and in none of the human isolates, while the MAT1-2 was detected in the other 10 of the animal isolates and in all of the human isolates. These results indicate that anthropophilic T. mentagrophytes evolved from the A. vanbreuseghemii (＋) mating strain.
More than 10 years have passed since Trichophyton tonsurans infection first began to increase in Japan. Initially the infection was confined to high school and university students participating in combat sports clubs, but it has now spread among the athletes' family members and friends. In a recent survey, 10% of Judo athletes tested positive for Trichophyton tonsurans; most were asymptomatic carriers. T.tonsurans infection usually causes tinea corporis or tinea capitis, but lesions can occur on other sites, causing tinea unguim, tinea manus, etc . The course of infection is usually only mildly symptomatic, and individuals with long-term infection can become asymptomatic carriers. It is likely that many individuals are unaware that they have Trichophyton tonsurans infection. The number of individuals infected with clear without repeating is difficult to assess due to the complexity of the fungal culture process. Diagnosis is made by direct examination in KOH and culture, and treatment consists of topical and / or oral antifungals. Prevention of Trichophyton tonsurans infection through increased awareness of the disease and careful hygiene is important.
An epidemiological survey of dermatomycoses and the causative fungus flora of dermatomycoses in Japan for 2006 was made on a total number of 63,029 outpatients who visited 16 dermatological clinics throughout Japan. The results were as follows.
1) Dermatophytosis was the most prevalent cutaneus fungal infection (7,582 cases) seen in these clinics, followed by candidiasis (842 cases) and then Malassezia infections (283 cases).
2) Among dermatophytoses, tinea pedis was the most frequent (4,779 cases : male 2,358, female 2,241), then in decreasing order, tinea unguium (2,582 cases : male 1,376, female 1,206), tinea corporis (564 cases : male 341, female 223), tinea cruris (309 cases : male 254, female 57), tinea manuum (145 cases : male 92, female 53), and tinea capitis including kerion (17 cases : male12, female 5).
3) Tinea pedis and tinea unguium are seen to increase in the summer season, among the aged population. When compared to the last survey 2002 by clinical form, t. unguium patients increased 459 cases.
4) As the causative dermatophyte species, Trichophyton rubrum was the most frequently isolated among all dermatophyte infections except tinea capitis. Microsporum canis was slightly increased. M.gypseum and Epidermophyton floccosum are small number. T.tonsurans was increased up to 37 cases.
5) Cutaneous candidiasis was seen in 842 cases (305 male, 537 female). Intertrigo (298 cases) was the most frequent clinical form, followed by erosion interdigitalis (136 cases), oral candidiasis (135 cases), onychia et paronychia (108 cases), genital and diaper candidiasis in total (88 cases).
6) Tinea versicolor was seen in 175 cases. Malassezia folliculitis were collected 108 cases, 63 cases are reported from one clinic.
Some yeast agents including Candida albicans, Candida tropicalis and Candida glabrata have a role in recurrent vulvovaginal candidiasis. We studied the frequency of both common and recurrent vulvovaginal candidiasis in symptomatic cases which were referred to Urmia Medical Sciences University related gynecology clinics using morphologic and molecular methods. The aim of this study was the identification of Candida species isolated from recurrent vulvovaginal candidiasis cases using a rapid and reliable molecular method.
Vaginal swabs obtained from each case, were cultured on differential media including cornmeal agar and CHROM agar Candida. After 48 hours at 37℃, the cultures were studied for growth characteristics and color production respectively. All isolates were identified using the molecular method of PCR - restriction fragment length polymorphism.
Among all clinical specimens, we detected 19 ( 16 % ) non fungal agents, 87 ( 82.1 % ) yeasts and 2 ( 1.9 % ) multiple infections. The yeast isolates identified morphologically included Candida albicans ( n = 62 ), Candida glabrata ( n = 9 ), Candida tropicalis ( n = 8 ), Candida parapsilosis ( n = 8 ) and Candida guilliermondii and Candida krusei ( n = 1 each ). We also obtained very similar results for Candida albicans, Candida glabrata and Candida tropicalis as the most common clinical isolates, by using PCR - Restriction Fragment Length Polymorphism. Use of two differential methods, morphologic and molecular, enabled us to identify most medically important Candida species which particularly cause recurrent vulvovaginal candidiasis.
This study investigated the effects of cinnamaldehyde in combatting the hyphal growth of Candida albicans under varying concentrations, treatment times, and temperatures to determine the potential benefits of applying this substance to anti-Candida foods or gargles. From the results of pretreatment with cinnamaldehyde against Candida hyphae, we found that its inhibitory activity seemed to be strengthened in parallel with prolonged pretreatment time and a rise in temperature, and that pretreatment of 2,000μg/ml for only 1 minute significantly inhibited the hyphal growth of C.albicans. We also demonstrated by XTT assay that pretreatment with cinnamaldehyde affected the metabolic activity of Candida hyphal cells. These findings suggest that a warm drink or mouthwash containing cinnamaldehyde might be a candidate as a prophylactic or therapeutic tool against oral Candida infection.
Onychomyosis caused by Aspergillus sydowii is rare and difficult to diagnose. We report a case in which this disease was diagnosed by molecular-biological methods. The patient was a 53-year-old woman without any notable past history. She visited our hospital complaining of itching around the right first toenail in June 2010, although she had noticed nail opacification for 4 years. Opaque thickening of the nail, covering 57.3% of the normal nail area, was observed. Direct microscopic examination revealed thick mycelia with septa and black spores. While colonies with a red brown center and a grayish blue-green margin were observed in culture on Sabouraud ' s glucose agar at 25℃, radially arranged conidia in the conidial head were observed in slide culture. Thus, onychomycosis caused by Aspergillus was suspected. There were no blood or biochemical test abnormalities. We directly extracted deoxyribonucleic acid from the nail and analyzed the base sequences of the internal transcribed spacer 1 and 2 regions of the ribosomal ribonucleic acid gene, and identified Aspergillus sydowii. Because the minimal inhibitory concentration of itraconazole (ITCZ) is 0.25μg/ml, we administered pulse therapy with monthly 1-week cycles of oral ITCZ 400 mg / day for 3 consecutive months. The opaque area subsided to 17. 9% of the normal nail by 6 months after treatment completion. However, 3 months later, the opaque area increased again to 22. 3%, and the same fungus was isolated and identified. The 3-month ITCZ pulse therapy was repeated and the symptoms disappeared, with complete cure achieved by 3 months after the second therapy. | 2019-04-22T19:18:29Z | https://www.jstage.jst.go.jp/browse/mmj/53/3/_contents |
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If you visit here at all regularly you'll be aware of my tendency to make stuff over and over until I find the perfect recipe. It's a personality flaw.
When I first met Adam, I made him chocolate self-saucing pudding. I think it was actually for our second date (chronology dependent on who you ask). He said he loved it. So I made it again. And again. And sometime after we were married (that would be SEVEN years later and heavens knows how many puddings) he confessed that he really didn't like chocolate self-saucing pudding. Any of them.
I halved this recipe for my own sake given I knew I would not throw it away.
And I figured: 21 weeks pregnant. Earned it.
Place butter in dish, melt in microwave. Stir in sifted flour, sugar and cocoa, milk and vanilla, beat until smooth. Sprinkle with combined sifted brown sugar and extra cocoa and pour boiling water over. Cook on high for 12 minutes or until just cooked in centre. Stand for a few minutes before serving because it will be piping hot. Awesome with icecream.
I love love love the chemistry of self saucing chocolate pudding. Pour on water – get a cake and sauce – MAGIC! And my Nan’s recipe is quite good too, and is the most used recipe in my scrapbook judging by the splashes of batter on it. Love your style – there is nothing worng with making a pudding just for yourself.
I love self saucing chocolate pudding. I had no idea you could make it in the microwave! Genius! I second the notion of making a pudding for yourself – I often make things just for me that Tom (husband to be) doesn’t like.
21 hours overtime? Do not halve that recipe, lady!!
Yummy! I love self saucing pud, haven’t had one in ages. And hey, being pregnant, I’m sure you could eat a whole batch easily!
Fi … you’ll never guess what I was inspired to make for dessert last night!!! I don’t have a computer at home, so found a recipe in one of Bill Granger’s book – it was pretty good. He also has a recipe for passionfruit puddings so I am going to try them this weekend. At this time of year I would normally use up all of the passionfruit making vodka 🙁 I thought you were meant to stop feeling like drinking alcohol when you were pregnant??? | 2019-04-19T06:26:19Z | https://buenavistafarm.com.au/chocolate-selfsaucing-pudding/ |
Best Buy customers often prefer the following products when searching for Freezer Compressor.
Browse the top-ranked list of Freezer Compressor below along with associated reviews and opinions.
"It is frost free...good for the price...You will not have to worry about your compressor overheating due to running too long....This freezer had everything I was looking for; shelves in the door, plenty of room, interior light, a lock and a indicator light on the outside so you can tell at a glance that the freezer is on"
"You will not have to worry about your compressor overheating due to running too long."
"the size is great...so far no problems...Energy use is low and the compressor is very quiet....Great freezer with all the room you will need I'm a avid deer hunter and this freezer holds a good amount of vacuum sealed meat and has a good seal you can hear the freezer make the seal once the door has been shut to assure a good seal on all the meat you've invested in"
"its a nice small freezer for a small family or for family need additional freezer beside their regular refrigerator, it has nice capacity and cooling is good to freeze items in short time, ideal for frozen meals, meats, icecreams...needed some extra freezer space so got this one and am very happy with this product the price was great and fits well in the garage cant beat it for extra freezer space it keeps everything frozen nicely"
"I research the freezer at kmart and best buy was cheaper by 4.00 I really love it its very deep and I am doing a lot of shopping so I am exicted and I am glad I invest in a freezer and yes I would recommend this freezer to friends family and to anyone...The wire baskets are a nice addition to this freezer because it allows you to organize and store your vegetables or meet in a nice separate section of the freezer"
"Got lots of room...Gets the job done...I just went to buy this freezer and I m so happy with it, so efficient nice and dipp and the most important thing for me is that this is so Quiet...The door is so much lighter and opens completely so you don't have to hold it open... So far we've been very pleased with how cold it is... Would definitely recommend it to anyone who needs extra freezer room"
"Seems to be good...LOTS of space too...Needed a new freezer as we had one over 20 years old, so we did a little research and found that this was one of the best rated...The speed that it freezes things is great, and the seal is the best on any fridge or freezer I've ever had"
"2nd one is great...I really love it...After looking at a few freezer options we choose this one over a similarly sized chest freezer and were not disappointed...Nice separations to be able to organize different food.Not frost free so it won't dry out food that stored"
"It is frost free...i luv my freezer...We purchased this freezer because we didn't have enough space in our bottom freezer refrigerator so we decided to go with an upright freezer and it was the best decision we made...We like the freezer and it does keep our food frozen and the panel on the front door is a good touch so we can see the status of the freezer"
"No issues at all...And is very quiet...This is a replacement deep freeze and the shelves are more sturdy and the layout is very practical to store and still be able to see what is in the freezer...Love the fact that it is a frost free upright which makes finding food so much easier"
"It works real well...No issues thus far...Love the light display showing if electric current is coming to freezer or no green light means power is out on freezer...Research confirmed the freezer would likely hold up, and while we haven't yet had triple digits I'm optimistic that the freezer will operate as well in August as it has since taking delivery a few months ago"
"It works real well...No issues thus far...Love the light display showing if electric current is coming to freezer or no green light means power is out on freezer...I just bought this freezer and so far, it has worked like a charm"
"I'd buy it again...it does cool well...Works well for me as a freezer but so far it has switched itself from freezer to fridge mode two times while the control panel was locked...We love that it can be a fridge or a freezer with the switch of a button"
"We also use it for extra ice cream, vegetables and meat." | 2019-04-21T18:07:50Z | https://www.bestbuy.com/site/shop/freezer-compressor |
A lot of intense design work went into the making of Sunset Overdrive for Xbox, making it that much more satisfying to recreate these digital weapons in the real world. Not only did Figure Plant have to match the look of the game, each piece had to be functional! With names like TNTeddy, Hack N Slay, Pyro Geyser, and the Roman Candle Gun you can imagine the kind of fun challenges we were up against. Here’s a few shots from the studio.
When you regularly convert vehicles and build custom mechanical objects, you end up with an assortment of interesting spare parts, making Figure Plant the perfect place to put these weapons together! | 2019-04-25T20:33:48Z | https://theartofdang.com/tag/sunset-overdrive/ |
Do you know how to find someone on Instagram by email? Or maybe you are looking for a way to find a person at Instagram with phone number or social media. Whatever it is, you can follow the method I am going to give next.
1/12/2013�� People say that no one can tell, but I've been looking at someones page and every time I look for their account they change their username. They also changed their profile to private after I've been looking at their page. I don't have an instagram account. | 2019-04-23T14:33:13Z | http://montrealpoutine.com/newfoundland-and-labrador/how-to-know-if-someone-looks-at-your-instagram.php |
A. CRG Global, Inc. has been in business with Nielsen for 24 years.
A. CRG Global, Inc. is a full-service data collection agency. We pride ourselves in assisting Nielsen with its data collection needs within our facilities located throughout the United States. In addition to recruitment and data collection, our corporate project management team provides quota oversight on a weekly basis.
A. Working with Nielsen the past 24 years has been an excellent opportunity for us as a full service research solutions provider. When speaking about the non-tangible benefits of this partnership, we always recognize the leadership position Nielsen has globally in the research industry. This association has elevated our company’s exposure to additional companies in need of research solutions and has cemented our reputation with our existing clients. Our firm has also established many processes and business protocols by working with a company of Nielsen’s magnitude. This has benefitted us both internally and externally with clients to provide them with the best possible research solutions for their individual needs.
A. The advice we would offer to other diverse owned businesses is to always remember the value that you bring to large corporations. Diversity provides a different point of view and we believe strongly that this is a distinguishing characteristic of our business. Being a certified woman-owned and lead organization offers many opportunities. We offer a consistent approach, detailed process, and outstanding customer service that has allowed us to grow to an industry leader and has opened up many doors. Large corporations expect the best, regardless of the diversity ownership, so we never take that for granted and strive to be the best provider and partner to all of our clients and we would encourage other diverse owned businesses to do the same. | 2019-04-20T14:57:22Z | https://www.nielsen.com/us/en/about-us/supplier-diversity/crg-global-inc.html |
Perfect medium elevation for your dream walkout cabin with nice lake views and privacy. The driveway is established, soil testing complete and on record for a conventional septic system. Electric and phone at the road. 220' meandering lake shoreline varies from muck to hard sand, some wetland due to creek that runs along the property line. Only 6.5 miles from Hayward. Certified Survey Map completed with ribbons marking the property lines.
220 feet of frontage on Nelson. The water is 33 ft deep and covers 2503 acres. | 2019-04-25T10:35:45Z | https://www.woodlanddevrealty.com/search/property.phtml/L/1506324/0_norway_point_hayward_wi/ |
A new social networking site has been set up to help people write books online. Despite the new forms of writing on the internet -blogs etc – books continue to hold sway over people’s minds. As such, this e-Publishing House could be quite successful.
Zazew – set up in Suffolk – is free but there is the understanding that the company will take a cut of the profits from books completed through the site.
The features the site offers: collaborative editing tools, writing feedback forums and opportunities to self-publish, are available elsewhere, but this site brings them together in a social network.
Authors can simply use it as a private online space to tinker with their work; they can open it up to a selected group of friends for feedback and editing; they can open it up to the whole network for comment or they can actively seek co-authors or collaborators for books they want help with.
It doesn’t have to be novels either: it can be any kind of book including novels, short stories, manuals, research papers, white papers, poetry and other documents.
Works can be sold as eBooks or printed and bound and sold on Amazon. They’re also running their own X-factor for authors competition.
From a browser’s point of view, the site is also interesting for a little read, in that compulsive way that myspace blogs are. | 2019-04-20T02:50:38Z | https://www.shinyshiny.tv/2009/11/zazew_-_a_socia.html |
Makano Surplus was established in 2012 offering a large range of stock for ex UK military wheeled and tracked vehicles such as collectable vehicles like Saracen, Saladin, Ferret, CVR(T)/W, Chieftain, 432, CET; and other track and wheeled variants. In addition to these vehicles, other equipment and vehicle type materials and spares are offered where available including marine and ground support parts.We work closely with our International shipping agent to transport, export and Import parts where needed with the all the legal paperwork in hand for an easy and safe transaction at either end. As a registered exporter, we can also arrange for any shipping license application such as OGEL, SIEL & EUU’s through our dedicated agent with offices situated around the world. We stock a large range of parts in our warehouse that are offered for sale ‘as is’ ‘where is’ in unused old stock or with service life remaining condition where stated.
To supply, source and deliver quality vehicle parts and goods to our customers within the UK, Europe and the rest of the world. | 2019-04-18T20:55:11Z | https://makanosurplus.co.uk/about-us/ |
Sometimes I look at a piece of code or a design or a problem that I want to solve and I can see the ultimate goal for how I want to approach it. I can see it very clearly. When this happens, I go straight to the end goal and do it the way that I can see it in my mind. When this happens it's great and I can get a tremendous amount accomplished in a very short time. It's great when it happens.
Unfortunately, it doesn't always happen that way.
Sometimes I look at a piece of code or a design or a problem and I know that it can be so much better. I know that there is a smooth and elegant way to approach it. I can feel it in my bones. I just can't see it yet.
The problem with these times when I know there is an elegant solution but I can't see it, is that it's like writers block for me. It will make me sit and stew and do nothing, because I can't quite formulate exactly what I want to do.
I've found that the best way to get past this writers block is to just force myself to do something. Anything. Just start with small changes, or pieces of code and gradually iterate through changing them and getting them closer and closer to where I need to end up.
It's like that scene in Finding Forrester where Sean Connery tells Rob Brown to just start typing. He starts with a paragraph from an existing paper, and then it starts to flow from himself.
Start with something. Even just a simple loop that has nothing in it. Get it to compile. Add more to it. Compile it, run it, extend it. It starts to flow. Then you can get it to do what you need it to do.
Iterate. Don't be afraid to start small and grow it into something bigger with many, many iterations. | 2019-04-19T04:29:12Z | http://helixsdk.org/qa/?qa=149/iteration-is-important |
If you're a Chinese food lover, then you don't need to go to China just to eat Chinese food. You will find abundant, delicious food that gives good impression, there is a great variety of food restaurant here in Davao that caters Chinese cuisine.
Chinese cuisine has a very high influence worldwide, it represents the magnificent culture they have almost a thousand old history. They are most famous with rice and wheat, millet and corn, buckwheat, potato, sweet potato, and many kind of legumes. Chinese dishes apart from rice, they are also most renowned in meals such as noodles, steamed bread, deep fry twisted dough sticks and siopao(steamed stuffed buns). You can enjoy delicious Chinese food not only in a high class restaurant but also ordinary eateries that serves quality service that are much cheaper but still serve delicious dishes.
DAVAO DENCIA'S RESTAURANT is famous with their TOKWA'T BABOY and LUGAW(congee with pork and tofu side dish).
A Chinese origin cook congee but when served with a side dish of tokwa't baboy it is definitely a Filipino meal.
NEW DAVAO FAMOUS RESTAURANT CO. One of the oldest typical Chinese restaurant here in Davao City. Is most famous with their own recipe of buttered chicken, Chopsuey, Chinese fried rice and my fave SATEMI.
A spicy noodles Chinese dish. Super hot!
DAVAO SHANGHAI RESTAURANT also one of the oldest Chinese restaurant here in Davao, famous for their crab omelette and century egg.
AHFAT SEAFOOD PLAZA. One of the best Chinese Restaurant here in Davao City. You must try their spicy spare ribs and my fave sizzling gambas(spicy prawn)!
SY BEE TIN BAKESHOPPE AND FOOD HAUS. The new addition for Chinese dish craving here in Davao, famous for their siopao and garlic bread.
Food here in Davao City as I say in my last post is not so hard to find, especially the cheapest one. There are so many choices and you will be the one to judge the taste.
Have a wonderful CHINESE NEW YEAR TO ALL!
It's nice to see that there are a lot of Chinese food in Davao. They also have a nice price range.
Happy Chinese New Year! I would love to try those Chinese food in Davao, it seems to be healthy and yum yum.
I do love eating Chinese foods. On this array of Chinese restos in Davao, I go for Sy Bee Tin. My wife and I really love eating siopao. Siopao is one of the Chinese food delicacies which we do crave to eat every time we see them.
Definitely all! Hahahaha...oh well...if you want me to choose one..I recommend sizzling gambas from AHFAT.
This will definitely satisfy my Chinese food cravings. I have many reasons to visit Davao City now. That crab omelet looks yummy.
I loooooove Satemi! Even though it's spicy but damn! The flavors are just so hard to resist that I would get another one! I would also love to try the Crab Omelet since I love crab and its taste. And who wouldn't love Ahfat with their tasty Chinese dishes feel like you're an emperor, eating all of the dishes in their round table!
Regarding foods addicts who may have still to test the initial taste involving Middle Western dishes, and also who want to eat the initial ethnic connection with eating at the Persia bistro, can get that will eating at one of these places to eat may be overpowering in the beginning. However, if you wish to make the most of eating at the Persia bistro, there are Restaurants tips to consider to be able to possess the very best knowledge probable at one of these shops. | 2019-04-19T22:18:18Z | http://www.foodiepalonline.com/2015/02/chinese-food-i-love-to-eat.html |
BASED IN BRISBANE TRAVELING FROM THE SUNSHINE COAST TO NORTHERN NEW SOUTH WALES.
I am a super suave 1952 caravan with a new lease on life.
I now serve alcohol at weddings and events posing as a mobile bar.
Copyright © 2018 hello mr Jenkins - All Rights Reserved. | 2019-04-19T03:00:01Z | https://hellomrjenkins.com/ |
This is a placeholder page for Carliegh Towsend, which means this person is not currently on this site. We do suggest using the tools below to find Carliegh Towsend.
You are visiting the placeholder page for Carliegh Towsend. This page is here because someone used our placeholder utility to look for Carliegh Towsend. We created this page automatically in hopes Carliegh Towsend would find it. If you are not Carliegh Towsend, but are an alumni of Hargrave High School, register on this site for free now. | 2019-04-22T16:26:41Z | http://hargravehighschool.org/alumni/3225637/carliegh-towsend.html |
The month of May marks an exciting day in the QLD Go Karting calendar with the Queensland Open State Titles run in conjugation with the 3rd Round of TLVKC Championship Season.
THREE DAYS OF RACING! - Hurry entries close 13th of May!
Friday 17th of May - Gates open 9am for pit set up with practice begin at 12pm and concluding at 5pm.
Saturday 18th of May - Gates open at 6 am, with drivers briefing at 8am with carby session and timed qualifying to follow at 8.30am.
Sunday 19th of May - Gates open at 6 am, with the running of Pre Final and Finals for all classes.
To form a class, MUST HAVE A MINIMUM OF 6 ENTRIES.
I am aware that participating in the Toowoomba & Lockyer Valley Kart Club Inc club competition is a potentially dangerous undertaking and that participating in that activity is at my own risk. I acknowledge that the Competition Organisers (which term includes all persons involved in the designing, construction and administering the competition, the host club, its Committee, members, servants or agents), cannot control a range of matters that will create or vary risks to my health and safety. Such things include the weather, deterioration of range surfaces through use, personal attributes such as my fitness and level of expertise and my approach to the challenges presented in this competition.
I am also aware that any person participating in the competition at this range is only allowed to do so on the distinct understanding that they do so at their own risk.
By accepting this document by checking the box below I understand that it is valid for the period 1st of January 2019 to 31st of December 2019. | 2019-04-24T02:22:33Z | https://www.eventbrite.com.au/e/queensland-open-state-titles-tickets-60038193854?aff=ehomecard&fbclid=IwAR3mhiXSzmrEv-TWXv9PEVf01C3kRxrTr09LiwTzmV0oAhckB0F_wxBOG90 |
Scown P E are Transport Operators providers. We help people in Taranaki. For more information, please call us today. | 2019-04-23T14:44:17Z | https://yellow.co.nz/y/scown-pe-upper |
Done! We’re proud to present an absolute highlight of a project: the Digital Signage Installation we did in line with the Nordkettenbahnen Media-Project.
The project demanded manifold channels and outputs. So we developed a tailor made application that is capable for this multi-platform distribution.
The Innsbruck Nordkettenbahnen Betriebs GmbH wanted to provide its guests with extended concierge and information services. In addition to a successful balance of information and emotion, the guests will be given an orientation aid on the mountain, to the respective tourist offers.
The individual chapters to the respective stations also show centrally collected information and dynamic live content.
We staged the athletes, who conquer the mountain with skis or ride the steep trails with the downhill bike during summer time. In other film parts the guest is shown the immediate proximity of the high-alpine scenario ("The Mountain in the City").
ARTFABRIK was responsible for the conception, the design-phase, programming, the creation of all contents - film parts & 3D / CGI - as well as the choreography of the Digital Signage Solution (cinematic). | 2019-04-24T03:48:42Z | https://artfabrik.at/innsbrucker-nordkettenbahnen-digital-signage.html |
Quicken Loans, Inc, is one of the reliable lending institutions that people can run to. A company founded during 1985, this was previously known as the Rock Financial, Corp. In 1999, they changed their name to Quicken Loans, Inc. offering mortgage loan solutions to those who want to purchase a home or keep the ones they are currently living in. The bank provides a loan modification service that matches no other. They even offer services online for retail and lender mortgages in the United States. Other services that are ready for borrowers are loan modification, refinancing, reverse mortgage, direct access to mortgage specialists, loan options home equities, real-time loan comparison, real-time loan status information and home-financing education.
This bank focuses on their refinancing division. As much as they can help people with their home mortgages, they will. As a result of this, they were able to come up with the Quicken Loans Mortgage Services. It is a type of loan modification program that improves the payment terms of their clients. For those outside the vicinity of the United States, they can enjoy this offer.
Banks in general are becoming stricter in approving loan modification applications. They have to make sure that they are very critical in making the decision to find out who is qualified or not. As the applicant, you have to do your part in finding out if you are qualified for this or not. You will find that although a loan modification may sound helpful, it may not necessarily be the one that is the perfect solution.
Quicken Loans Mortgage Services will be able to deliver service as you can expect it. For the longest time, they are known to provide customers with only the best. This also applies for this program too. Clients will be able to choose from the different options for a loan modification. No matter what it is that they are experiencing, they will be able to start working with the right loan modification program from Quicken Loans Mortgage Services.
Recently, they have joint business with the Fortress Investment Group LCC. The public has been waiting for what comes with this joint venture as is it a big step taken. The bank has carefully strategized refinance options for customers. They make sure that payment term adjustments are made properly. Two companies will share equal benefit from their loan modification programs. They also make sure that borrowers will benefit as well. | 2019-04-18T18:34:58Z | https://www.foreclosureprocess.org/quicken-loans-inc/ |
All information and specifications shown on this website are based upon the latest available information provided by race organizations, teams and riders. No rights can be withdrawn from rankings, results or statistics as presented on this website. 0.08026 sec. | 2019-04-23T22:49:43Z | https://www.procyclingstats.com/race/okolo-jiznich-cech/2018/stage-1 |
Asia’s #1 Favourite Anisong artiste, LiSA, holds first solo concert in Singapore!
Singapore, 30 June 2015 – With less than one month before LiSA’s concert in Singapore titled “LiVE is Smile Always ~ Singapore 2015 ~” on the 18th of July at the Resorts World™ Theatre, Resorts World™ Sentosa.
As part of the highly demanded LiVE is Smile Always Asia tour to be held in Hong Kong, Taipei, Shanghai and Singapore. Performing her first solo live in Singapore on the 18th of July. With an extensive list of theme songs from hugely popular anime titles from Fate/Zero and Sword Art Online, LiSA is set to wow the audiences together with her infectious, energetic live performances.
2015 also marks the first time LiSA has performed in Mexico, USA and The Philippines.
LiVE is Smile Always ~ Singapore 2015 ~ is proudly organised by SOZO in partnership with Sony Music Artists , under the Japan Music Festival platform.
Tickets for the concert are now on sale via SISTIC, and VIP tickets are SOLD OUT on shortly after the start of ticketing sales. With only limited number of General Access (GA) tickets priced at S$98 on sale, consumers are encouraged to purchase tickets early to avoid disappointment. Prices do not include ticketing charges and entry into Sentosa.
No Comment to " LISA ~ LiVE is Smile Always Prelude " | 2019-04-22T00:21:08Z | https://www.milkcananime.com/2015/07/lisa.html |
Home » About Us » How does it work?
Members join – buying a share in Sastak, register any machinery, labour, services, commodities etc they may have available and this goes on to Sastak’s central database.
A customer (member or non-member) requiring a particular service or product contacts the Ring office.
The office will contact suppliers to find one available to carry out the work or supply the product required.
The customer is advised which supplier is offering the required service/product.
There is no obligation for a supplier to undertake a job or let a machine go out on hire.
The customer/supplier make arrangements and the job is completed.
A customer can request a specific supplier or state who he or she does not wish to use.
Supplier completes simple worksheet, agreed and signed by the client – and sends to Sastak.
Sastak produces 2 invoices including suppliers VAT etc.
a) To customer – total plus the agreed commission.
b) To supplier – total minus the agreed commission.
Payment collected by Direct Debit from Client within 21 days.
The supplier is paid by Direct Credit in to bank account in 30 days.
Sastak acts as an agent only and not as principle.
Labour payment terms differ and are collected in 7 days and paid out in 10 days.
Other payment terms can be made by arrangement. | 2019-04-23T07:54:35Z | https://sastak.com/about-us/how-does-it-work/ |
The Clarke Nicklin Group will make a new start this New Year as the team relocates from Hazel Grove to a prestigious new development at Cheadle Royal Business Park.
The new building, developed by Muse Developments, is synonymous with the company’s fresh culture and ethos and reflects the Group’s plans to extend its presence in the region, and become more accessible for clients. It will also help accommodate the rapid growth through which the business has gone, with the accountancy practice doubling in size in just four years. | 2019-04-19T08:20:37Z | http://www.clarkenicklin.co.uk/news/12-news/68-feb-10-office-move |
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