IdA string | IdB string | labels int64 | mechanism string | effect string | score float64 | sentence string | signor_id string |
|---|---|---|---|---|---|---|---|
P35222 | Q05513 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.599 | Yap and β-catenin are direct substrates of PKCζ. Similar MS/MS analysis to map the sites phosphorylated in β-catenin by PKCζ identified S45 and several sites of low abundance that included S552 and S675 (Figure S3C). | SIGNOR-276880 |
Q15139 | P35222 | 1 | phosphorylation | up-regulates | 0.388 | This study provides evidence that pkd1 interacts with and phosphorylates beta-catenin at thr(112) and thr(120) we postulate that pkd1 phosphorylation is required to maintain _-catenin transcription activity. | SIGNOR-183384 |
P12931 | P46527 | 1 | phosphorylation | down-regulates | 0.496 | Src regulates p27 stability through phosphorylation of p27 at tyrosine 74 and tyrosine 88. Our data indicate that phosphorylation by src impairs the cdk2 inhibitory action of p27 | SIGNOR-152839 |
P19484 | P10619 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.298 | Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy|A chromatin immunoprecipitation (ChIP) assay with antibodies against TFEB or ACSS2 demonstrated that glucose deprivation results in the binding of TFEB (Figure 3D) and ACSS2 (Figure 3E) to the promoter regions of CTSA, GBA, GU... | SIGNOR-276549 |
Q9NQU5 | P35222 | 1 | phosphorylation | down-regulates quantity | 0.2 | Moreover, we find that \u03b2-catenin is also localized with PAK6 in cell-cell junctions and is a novel PAK6 substrate.|PAK6 binds to and phosphorylates beta-catenin. | SIGNOR-279546 |
P46531 | Q13233 | 0 | phosphorylation | down-regulates activity | 0.367 | As a result, MEKK1 suppresses the Notch1 intracellular domain protein stability and transcriptional activity.|We confirmed that MEKK1 binds to Notch1 intracellular domain and phosphorylates the Notch1 intracellular domain Threonine 2512 residue. | SIGNOR-279628 |
O96013 | Q9BY11 | 1 | phosphorylation | up-regulates activity | 0.295 | We identified two novel Pak5 substrates, Pacsin1 and Synaptojanin1, proteins that directly interact with one another to regulate synaptic vesicle endocytosis and recycling. Pacsin1 and Synaptojanin1 were phosphorylated by Pak5 and the other group II Paks in vitro, and Pak5 phosphorylation promoted Pacsin1-Synaptojanin1... | SIGNOR-263023 |
P29375 | P68431 | 1 | demethylation | up-regulates activity | 0.2 | KDM5 subfamily is capable of removing tri‐ and di‐ methyl marks from lysine 4 on histone H3 (H3K4). Depending on the methylation site, its effect on transcription can be either activating or repressing. | SIGNOR-264299 |
P24941 | Q06609 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.595 | Phosphorylation of the BRCA2 C-terminal RAD51 binding site by CDK2 promotes RAD51 filament disassembly, leading to nucleolitic cleavage of newly synthesized DNA and compromised fork integrity. | SIGNOR-280213 |
O60858 | P31751 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.2 | Here, we demonstrate that overexpression of RFP2 in cells induced apoptosis through proteasomal degradation of MDM2 and AKT. We observed that RFP2 formed a complex with MDM2, a negative regulator of the p53 tumor suppressor, and AKT, a regulator of apoptosis inhibition at the cellular level. Additionally, we found tha... | SIGNOR-271853 |
Q9Y222 | Q9NZW4 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.2 | In addition, our in vitro analyses showed that DMP1 and its 57-kDa C-terminal fragment significantly up-regulated the Dspp promoter activities in a mesenchymal cell line. | SIGNOR-271685 |
Q9HCU5 | P29376 | 0 | phosphorylation | down-regulates activity | 0.2 | Furthermore, we show that LTK interacts with and phosphorylates Sec12. Expression of a phosphoablating mutant of Sec12 reduces the efficiency of ER export. Thus, LTK-to-Sec12 signaling represents the first example of an ER-resident signaling module with the potential to regulate proteostasis.Altogether, we propose that... | SIGNOR-273650 |
Q00535 | O60260 | 1 | phosphorylation | down-regulates | 0.2 | Phosphorylation by cdk5 decreased the auto-ubiquitylation of parkin both in vitro and in vivo. | SIGNOR-153445 |
O15119 | Q8N726 | 1 | transcriptional regulation | down-regulates quantity by repression | 0.395 | TBX2 and TBX3 function as transcriptional repressors and both have been shown to inhibit myogenesis (Carlson et al, 2002; Zhu et al, 2014). Abnormal expression of TBX2 has been reported in several cancers including breast, pancreas, and melanoma, where it has been shown to drive proliferation (reviewed in Abrahams et a... | SIGNOR-249603 |
O75365 | P15311 | 1 | dephosphorylation | down-regulates activity | 0.277 | Here we report the identification of Ezrin as a specific and direct cellular substrate of PRL-3. In HCT116 colon cancer cell line, Ezrin was identified among the cellular proteins whose phosphorylation level decreased upon ectopic over-expression of wtPRL-3 but not of catalytically inactive PRL-3 mutants. Although PRL-... | SIGNOR-248342 |
P45984 | P16949 | 1 | phosphorylation | down-regulates | 0.256 | Involved in the regulation of the microtubule (mt) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Here we show that in response to hyperosmotic stress, jnk phosphorylates a key cytoplasmic microtubule regulatory protein, stathmin (stmn), on conserved ser-25 an... | SIGNOR-166698 |
O00429 | P06493 | 0 | phosphorylation | up-regulates activity | 0.466 | Drp1 is phosphorylated at the Ser616 position and activated predominantly by CDK1. | SIGNOR-279394 |
Q8TEP8 | P53350 | 0 | phosphorylation | up-regulates activity | 0.608 | In the presence of AurA binding, Plk1 preferentially phosphorylates and interacts with the T44 motif of Cep192 through the “self-priming and binding” mechanism | SIGNOR-266405 |
P55075 | P19622 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.416 | Our results in ES cells suggest that Engrailed inhibits Fgf8 expression in the absence of Pbx1. We identified single Engrailed- and Pbx-binding sites in the Fgf8 intron that inhibit expression of Fgf8 in mouse ES cells, but that together can allow full Fgf8 expression. | SIGNOR-265801 |
Q9Y2Y9 | Q13523 | 0 | phosphorylation | down-regulates | 0.342 | Using yeast two-hybrid screening of a human thymus cdna library, prp4, a serine/threonine protein kinase, was identified as a klf13-binding protein...coexpression of prp4 and klf13 increases nuclear localization of klf13 and ccl5 transcription. | SIGNOR-154951 |
P12931 | P29353 | 1 | phosphorylation | up-regulates activity | 0.666 | Here, we report the identification of two major and novel Shc tyrosine phosphorylation sites, Y239 and Y240. Y239/240 are co-ordinately phosphorylated by the src protein-tyrosine kinase in vitro, and in response to epidermal growth factor stimulation or in v-src-transformed cells in vivo. phosphorylation of y317 has b... | SIGNOR-44870 |
Q2TAL8 | Q5JPH6 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.2 | QRICH1 promotes the expression of translation-related genes. our combined ChIP-seq and RNA-seq analyses identified that QRICH1 and ATF4 were enriched at the promoters of these specific tRNA synthetases, and that ER stress positively regulated their transcription (Fig. 4I). Together, these findings suggest that QRICH1 a... | SIGNOR-269402 |
Q13009 | P12931 | 0 | phosphorylation | up-regulates | 0.657 | Tiam1 cooperated with src to induce activation of rac1 in vivo and the formation of membrane ruffles. | SIGNOR-102354 |
P35222 | Q15139 | 0 | phosphorylation | up-regulates | 0.388 | This study provides evidence that pkd1 interacts with and phosphorylates beta-catenin at thr(112) and thr(120) we postulate that pkd1 phosphorylation is required to maintain _-catenin transcription activity. | SIGNOR-183384 |
P49336 | P51946 | 1 | phosphorylation | down-regulates | 0.645 | Cdk8 phosphorylates mammalian cyclin h in the vicinity of its functionally unique amino-terminal and carboxy-terminal alpha-helical domains. This phosphorylation represses both the ability of tfiih to activate transcription and its ctd kinase activity | SIGNOR-82033 |
Q06187 | P42336 | 0 | phosphorylation | up-regulates activity | 0.514 | Activation of Btk occurs by transphosphorylation of tyrosine 551 in the catalytic domain, resulting in a dramatic increase in the catalytic activity of the kinase (11, 12, 13). This allows for autophosphorylation at tyrosine 223 in the SH3 domain (14). Both Lyn and Syk have been demonstrated to be involved in BCR-media... | SIGNOR-249610 |
P28845 | P17676 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.284 | In conclusion, C/EBPalpha and C/EBPbeta control basal transcription, and TNF-alpha upregulates 11beta-HSD1, most likely by p38 MAPK-mediated increased binding of C/EBPbeta to the human HSD11B1 promoter. | SIGNOR-268972 |
P84022 | Q13705 | 0 | phosphorylation | up-regulates activity | 0.69 | It has been suggested that binding of myostatin to the ActRIIB results in the phosphorylation of two serine residues of Smad2 or Smad3 at COOH domains | SIGNOR-254985 |
O43583 | O60674 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.2 | DENR directly regulates JAK2 expression. | SIGNOR-269675 |
P49674 | P12830 | 1 | phosphorylation | down-regulates activity | 0.259 | Casein kinase 1 is a novel negative regulator of E-cadherin-based cell-cell contacts|CK1 colocalizes with E-cadherin and phosphorylates the cytoplasmic domain of E-cadherin in vitro and in a cell culture system. We show that the major CK1 phosphorylation site of E-cadherin is serine 846 | SIGNOR-274047 |
P60568 | O95644 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.565 | Together, our results demonstrate that dnNFAT inhibits the production of IL-2. Thus, the NFAT transcription factor contributes to the regulation of IL-2 gene expression and therefore plays a critical role in the initiation of immune responses. | SIGNOR-275405 |
P10636 | Q15759 | 0 | phosphorylation | down-regulates activity | 0.341 | Phosphorylation of tau by SAPK3 and SAPK4 resulted in a marked reduction in its ability to promote microtubule assembly.|Tau phosphorylated by SAPK2b and SAPK2a also reacted with AT8, whereas AT8 failed to recognise tau phosphorylated by SAPK1gamma. | SIGNOR-279637 |
P30281 | P49841 | 0 | phosphorylation | down-regulates | 0.435 | We have previously shown that both basal and camp-induced degradation of cyclin d3 in reh cells is dependent on thr-283 phosphorylation by glycogen synthase kinase-3beta (gsk-3beta). | SIGNOR-142880 |
Q99814 | Q9Y2K7 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.2 | To this end, we confirm that KDM3A, KDM4B, KDM4C, KDM5B, KDM5C, and KDM62 are direct targets of HIF-1a while extent the list of known targets to KDM2A, KDM2B, KDM4D, KDM5A, and KDM6A. The results demonstrated that majority of the KDMs were similarly induced (KDM2A, KDM2B, KDM3A, KDM4B, KDM4C, KDM4D, KDM5A, KDM5B, KDM5C... | SIGNOR-271581 |
Q92481 | P02511 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.2 | Aberrant expression of CRYAB has been shown to be associated with several neurological diseases and malignant neoplasms. To identify transcriptional regulators of CRYAB expression, we examined its promoter for binding sites of transcription factors and identified four potential AP-2 binding sites in addition to a p53 b... | SIGNOR-253637 |
Q16665 | Q9UGL1 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.271 | To this end, we confirm that KDM3A, KDM4B, KDM4C, KDM5B, KDM5C, and KDM62 are direct targets of HIF-1a while extent the list of known targets to KDM2A, KDM2B, KDM4D, KDM5A, and KDM6A. The results demonstrated that majority of the KDMs were similarly induced (KDM2A, KDM2B, KDM3A, KDM4B, KDM4C, KDM4D, KDM5A, KDM5B, KDM5C... | SIGNOR-271563 |
P43405 | Q32MZ4 | 1 | phosphorylation | up-regulates activity | 0.2 | However, this inhibitory activity TRIP can be reversed by the co-expression of Syk, which might inhibit the activity of cytoplasmic TRIP, sequester TRIP from important nucleolar targets or even counter TRIP 's inhibitory effects on TRAF and TNF signaling by regulating the activity of alternative components of the pathw... | SIGNOR-279297 |
Q05513 | Q8N2W9 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.519 | In this study, we discovered a new protein isoform encoded by KIAA0317, termed fibrosis-inducing E3 ligase 1 (FIEL1), which potently stimulates the TGFbeta signaling pathway through the site-specific ubiquitination of PIAS4.FIEL1 targets PIAS4 using a double locking mechanism that is facilitated by the kinases PKCzeta ... | SIGNOR-275513 |
Q15418 | Q96QZ7 | 1 | phosphorylation | up-regulates activity | 0.286 | We report herein that p90RSK associates with MAGI1 in ECs and executes 2 independently regulated PTMs of MAGI1: S741 phosphorylation and K931 deSUMOylation. MAGI1-S741 phosphorylation is vital for Rap1 activation. | SIGNOR-273837 |
Q05469 | P27361 | 0 | phosphorylation | up-regulates activity | 0.415 | Thus, activation of the ERK pathway appears to be able to regulate adipocyte lipolysis by phosphorylating HSL on Ser(600) and increasing the activity of HSL. | SIGNOR-249470 |
P51948 | P04637 | 1 | ubiquitination | down-regulates quantity by destabilization | 0.351 | Collectively, these results indicate that MNAT1 increases p53 ubiquitination, thus promoting its proteasomal degradation.|These suggest that MNAT1 decreases p53 expression by the proteasome. | SIGNOR-278831 |
Q9H0H5 | Q96GD4 | 0 | phosphorylation | up-regulates activity | 0.78 | It was found that the 5A fragment in which five Ser/Thr residues were substituted with Ala (S144A/T145A/S185A/T186A/S187A) fully prevented phosphorylation (Fig. 5B), confirming that Aurora B primarily phosphorylates five Ser/Thr residues in the basic region of MgcRacGAP. | the strong phosphorylation of the basic region... | SIGNOR-250590 |
P17174 | P17676 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.2 | In cotransfection experiments, the C/EBP beta protein trans-activated 10-15-fold the cAspAT gene promoter in HepG2 cells. Deletion studies revealed that regions P2 and P4 are critical for promoter activity. In gel retardation experiments, the P4 region bound different C/EBP-related proteins in different tissues | SIGNOR-254051 |
Q9H6R7 | P53350 | 0 | phosphorylation | up-regulates activity | 0.2 | PLK1 Phosphorylates MMAP to Promote Its Interaction with KIF2A and MRE11. we performed in vitro kinase assays followed by mass spectrometry and found that two sites (S686 and S695) in this cluster were phosphorylated. Thus, all of these results are in agreement that this cluster is phosphorylated by PLK1. | SIGNOR-273730 |
P49589 | P18848 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.2 | QRICH1 promotes the expression of translation-related genes. our combined ChIP-seq and RNA-seq analyses identified that QRICH1 and ATF4 were enriched at the promoters of these specific tRNA synthetases, and that ER stress positively regulated their transcription (Fig. 4I). Together, these findings suggest that QRICH1 a... | SIGNOR-269416 |
P46734 | O15264 | 1 | phosphorylation | up-regulates activity | 0.607 | p38-δ is activated by environmental stress, extracellular stimulants, and MAPK kinase-3, -4, -6, and -7. we investigated whether this Thr180-Gly-Tyr182 motif was essential for p38-δ activation. Taken together, these results suggest that the dual phosphorylation TGY motif is required for p38-δ activation. | SIGNOR-273950 |
P00748 | P01008 | 0 | cleavage | down-regulates activity | 0.6 | Antithrombin (AT), a member of the serine protease inhibitor (SERPIN) superfamily, is a major circulating inhibitor of blood coagulation proteases such as factor (F) IIa (known as thrombin), FXa and, to a lesser extent, FIXa, FXIa and FXIIa. SERPINC1, which encodes AT in humans, is located on chromosome 1q25.1 | SIGNOR-264139 |
Q13315 | Q09472 | 1 | phosphorylation | up-regulates | 0.4 | Atm mediates phosphorylation of p300 in response to dna damageexpression of nonphosphorylatable serine to alanine form of p300 (s106a) destabilized both p300 and nbs1 proteins, after dna damage | SIGNOR-165567 |
Q96EB6 | P35222 | 1 | deacetylation | up-regulates quantity | 0.505 | SIRT1 deacetylates β-catenin to promote its accumulation in the nucleus and thus induces the transcription of genes that block MSC adipogenesis. | SIGNOR-256208 |
Q96TA1 | P15056 | 0 | phosphorylation | down-regulates activity | 0.2 | Overall, this indicates that BRAF-dependent phosphorylation of FAM129B controls its cellular localization and thus its ability to bind to KEAP1 to block NRF2 degradation. | SIGNOR-279595 |
P67870 | P35222 | 1 | phosphorylation | up-regulates activity | 0.601 | We show that CKII phosphorylates the N-terminal region of beta-catenin and we identified Ser29, Thr102, and Thr112 as substrates for the enzyme. We provide evidence that CKII regulates the cytoplasmic stability of beta-catenin and acts synergistically with GSK-3beta in the multi-protein complex that controls the degrad... | SIGNOR-251067 |
O14763 | P25490 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.403 | Depletion of FKBP51 impairs the acetylation status of YY1 and interferes with its binding on the DR5 promoter. The lack of the repressor activity of YY1 increases DR5 transcription and sensitizes melanoma cell to TRAIL-induced apoptosis. | SIGNOR-268793 |
Q9HAZ1 | Q13285 | 1 | phosphorylation | up-regulates activity | 0.2 | Immunoblotting analyses showed that the phosphorylation status of NR5A1 at Ser203 was attenuated by the CLK1/4 inhibitor. | SIGNOR-274117 |
Q14106 | P06493 | 0 | phosphorylation | up-regulates activity | 0.2 | Taken together, these observations strongly support the notion that several different CDK-cyclin complexes are involved in the phosphorylation of Tob2 at S254.A more detailed regulatory context of Tob2 phosphorylation at S254 is provided by our findings from mass-spec and in vitro kinase analyses that suggest connectio... | SIGNOR-273591 |
O75093 | Q14938 | 0 | transcriptional regulation | up-regulates quantity | 0.2 | For example, within the NFI targetome, we identified 6 collagen genes, 13 genes encoding potassium channel or glutamate receptor subunits and a range of factors related to axon guidance (e.g. Slit1, Robo1, Epha4, Epha5, Epha8) | SIGNOR-268906 |
P35236 | Q15139 | 0 | phosphorylation | up-regulates activity | 0.2 | HePTP is phosphorylated by PKC isozymes at Ser-225 in vitro. While all isozymes phosphorylated Ser-225 predominantly and Ser-113 to a lesser extent (Fig. (Fig.5),5), they differed strikingly in how much 32P they incorporated into HePTP during the 30-min assay. PKC θ was the most efficient, while PKC ζ and PKC μ were c... | SIGNOR-276046 |
Q05655 | O76090 | 1 | phosphorylation | down-regulates activity | 0.2 | We have identified a PKC phosphorylation site (S358) located in the C terminal region of hBest1 critical for channel rundown. Phosphorylation of this site by PKC activators and PP2A inhibitors reduces channel rundown. | SIGNOR-260880 |
P49841 | P15260 | 1 | phosphorylation | up-regulates quantity by stabilization | 0.2 | Our data suggest that glycogen synthase kinase 3 beta (GSK3beta) phosphorylates IFNGR1 thereby enhancing receptor protein stability by limiting ubiquitin proteasomal degradation. | SIGNOR-279720 |
P52292 | Q14653 | 1 | relocalization | up-regulates activity | 0.2 | The results from Figure 1C suggest that ORF6 inhibits IFN-β production through IRF3 or a component downstream of IRF3. Thus, we examined the effect of ORF6 on IRF3 nuclear translocation. Upon poly(I:C) treatment, IRF3 translocated to the cell nucleus in the absence of ORF6, whereas the expression of ORF6 blocked its nu... | SIGNOR-262514 |
P03372 | Q14164 | 0 | phosphorylation | up-regulates | 0.341 | Here, we show that ikkepsilon interacts with and phosphorylates estrogen receptor alpha (eralpha) on serine 167 in vitro and in vivo. As a result, ikkepsilon induces eralpha transactivation activity and enhances eralpha binding to dna. | SIGNOR-161834 |
Q9UL62 | P17612 | 0 | phosphorylation | down-regulates quantity | 0.2 | Together, these results suggest that TRPC5 is directly phosphorylated by G(s)/cAMP/PKA at positions S794 and S796. These inhibitory effects were blocked by the protein kinase A (PKA) inhibitors, KT-5720 and H-89, as well as by two point mutations at consensus PKA phosphorylation sites on TRPC5 (S794A and S796A). | SIGNOR-277823 |
P49815 | P49840 | 0 | phosphorylation | up-regulates | 0.362 | Gsk3 inhibits the mtor pathway by phosphorylating tsc2 in a manner dependent on ampk-priming phosphorylation. | SIGNOR-149377 |
O60285 | P55212 | 1 | phosphorylation | down-regulates activity | 0.483 | ARK5 negatively regulates procaspase-6 by phosphorylation at Ser257, leading to resistance to the FasL/Fas system. | SIGNOR-250209 |
P05771 | P61764 | 1 | phosphorylation | down-regulates activity | 0.398 | Munc18a is essential for neurotransmitter release by exocytosis and can be phosphorylated by PKC in vitro on Ser-306 and Ser-313. We demonstrate that it is phosphorylated on Ser-313 in response to phorbol ester treatment in adrenal chromaffin cells. Mutation of both phosphorylation sites to glutamate reduces its affini... | SIGNOR-249186 |
O14965 | Q96CG3 | 1 | phosphorylation | up-regulates activity | 0.2 | Here, we report that Aurora A is essential for Thr9 phosphorylation of the TRAF-interacting protein TIFA, triggering activation of the NF-κB survival pathway in AML. | SIGNOR-273551 |
O14920 | P04637 | 1 | phosphorylation | up-regulates activity | 0.52 | Here , we show that IKKbeta modulates the activity of p53 in response to glutamine depletion to promote cancer cell adaptation .|Taken together, these results indicate that IKK\u03b2 phosphorylates p53 on Ser392 as an early response to glutamine deprivation and possibly later facilitates its phosphorylation at Ser15 an... | SIGNOR-278516 |
P29590 | P27361 | 0 | phosphorylation | up-regulates | 0.339 | Phosphorylation of pml by mitogen-activated protein kinases plays a key role in arsenic trioxide-mediated apoptosis. | SIGNOR-124317 |
P46531 | Q9BWU1 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.302 | Mapping of cyclin C-dependent phosphosites on ICN1, using mass spectrometry revealed that several of them are located within the PEST-domain of Notch1, which controls ICN1 degradation38,39 (Fig. 5g and Supplementary Table 1). Three of them (T2512, S2514 and S2517) are localized within the consensus motif, “Cdc4 phospho... | SIGNOR-273135 |
Q09472 | Q9H2X6 | 0 | phosphorylation | up-regulates activity | 0.611 | Furthermore, HIPK2 forms a complex with the coactivator p300 and AML1, phosphorylates p300 at multiple Serine/Threonine sites and activates p300 HAT activity and coactivator function. | SIGNOR-278943 |
P06493 | Q9H1E3 | 1 | phosphorylation | down-regulates activity | 0.474 | putative phosphorylation site for Cdk1 is present in the DNA-binding domain peptide. This site, corresponding to Ser 181 in the NUCKS primary structure, is phosphorylated in vitro by Cdk1 with a Km of approximately 35 μM [7]. Phosphorylation of Ser 181 in the synthetic, DNA-binding domain peptide reduces its affinity f... | SIGNOR-261959 |
P43405 | P46934 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.291 | The latent membrane protein (LMP) 2A of Epstein-Barr virus (EBV) is implicated in the maintenance of viral latency and appears to function in part by inhibiting B-cell receptor (BCR) signaling. LMP2A enhances Lyn and Syk ubiquitination in vivo in a fashion that depends on the activity of Nedd4 family members and correl... | SIGNOR-272581 |
Q00535 | P26358 | 1 | phosphorylation | up-regulates | 0.358 | We report that cyclin-dependent kinases (cdks) 1, 2 and 5 can phosphorylate ser154 of human dnmt1 in vitro. Further evidence of phosphorylation of endogenous dnmt1 at position 154 by cdks is also found in 293 cells treated with roscovitine, a specific inhibitor of cdk1, 2 and 5 | SIGNOR-173685 |
P31260 | Q06124 | 0 | dephosphorylation | up-regulates | 0.373 | We also identified hoxa10 as a substrate for shp2 in undifferentiated myeloid cells, an effect that diminished during myelopoiesis. However, a constitutively active form of shp2 dephosphorylated hoxa10 throughout ex vivo myelopoiesis and sustained repression of hoxa10 target genes involved in phagocyte effector functio... | SIGNOR-182475 |
Q9P0J1 | P11362 | 0 | phosphorylation | down-regulates activity | 0.292 | Here we report that phosphorylation at another tyrosine residue, Tyr-94, inhibits PDP1 by reducing the binding ability of PDP1 to lipoic acid, which is covalently attached to the L2 domain of dihydrolipoyl acetyltransferase (E2) to recruit PDP1 to PDC. We found that multiple oncogenic tyrosine kinases directly phosphor... | SIGNOR-276640 |
O95831 | Q9NQU5 | 0 | phosphorylation | down-regulates activity | 0.2 | Furthermore, PAK5 phosphorylates AIF at Thr281 site to inhibit the formation of AIF/importin \u03b13 complex, leading to decrease AIF nuclear translocation.|These results suggested that PAK5 can inhibit AIF from entering the nucleus and prevent caspase- independent apoptosis. | SIGNOR-278969 |
Q86Y13 | P0C0S5 | 1 | monoubiquitination | up-regulates activity | 0.2 | 2A-HUB catalyzes monoubiquitination of H2A at lysine 119, functioning as a combinatoric component of the repression machinery required for specific gene regulation programs. Thus, 2A-HUB mediates a selective repression of a specific set of chemokine genes in macrophages, critically modulating migratory responses to TL... | SIGNOR-271748 |
Q9BV73 | P51955 | 0 | phosphorylation | down-regulates | 0.774 | C-nap1 hyperphosphorylation triggers the loss of both oligomerization and, crucially, interaction with the core centriole proximal-end protein, cep135. All three of these sites were identified in our in vivo analysis but only two (s2234 and s2394) were identified as nek2 phosphorylation sites in vitro. | SIGNOR-204837 |
Q9NWD9 | P53350 | 0 | phosphorylation | up-regulates activity | 0.2 | In addition, the inhibition of PLK1 activity by BI2536 treatment sharply reduced BEX4 protein, which was localized at the centrosomes in the HeLa cells or the GFP-BEX4 stable cell lines.|PLK1 directly phosphorylates BEX4 at T107 and contributes to BEX4-induced aneuploidy. | SIGNOR-279550 |
P28482 | Q05923 | 0 | dephosphorylation | down-regulates | 0.745 | Pac1 and mkp-1 previously have been implicated in the in vivo inactivation of erk or of erk and jnk, respectively. | SIGNOR-40915 |
P49327 | Q6Y1H2 | 0 | chemical activation | up-regulates activity | 0.2 | Very long-chain fatty acids are produced through a four-step cycle. However, the 3-hydroxyacyl-CoA dehydratase catalyzing the third step in mammals has remained unidentified. Mammals have four candidates, HACD1-4, based on sequence similarities to the recently identified yeast Phs1, although HACD3 and HACD4 share relat... | SIGNOR-267761 |
P14652 | P32243 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.259 | Transactivation of the mouse OTX2 Luc constructs by the human HOXB1, HOXB2, and HOXB3 proteins. | Likewise, the construct pOTX2LucΔ−710 showed an 8-, 12-, and 6-fold increase in transcriptional activity if co-transfected with pSG-HOXB1, -HOXB2, and -HOXB3, respectively | SIGNOR-261634 |
P78527 | Q13426 | 1 | phosphorylation | up-regulates activity | 0.907 | In response to ionizing radiation, ATM phosphorylates FBXW7 at serine 26 to recruit it to DNA double-strand break (DSB) sites, whereas activated DNA-PKcs phosphorylates XRCC4 at serines 325/326, which promotes binding of XRCC4 to FBXW7. SCF(FBXW7) E3 ligase then promotes polyubiquitylation of XRCC4 at lysine 296 via ly... | SIGNOR-277198 |
P37840 | P48729 | 0 | phosphorylation | up-regulates | 0.371 | In vitro experiments and two-dimensional phosphopeptide mapping provided further evidence that serine 129 was phosphorylated by ck-1 and ck-2. Moreover, phosphorylation of serine 129 was reduced in vivo upon inhibition of ck-1 or ck-2. These data demonstrate that alpha-synuclein is constitutively phosphorylated within ... | SIGNOR-73799 |
P02462 | Q8NBJ5 | 0 | glycosylation | up-regulates activity | 0.402 | Recombinant GLT25D1 and GLT25D2 enzymes showed a strong galactosyltransferase activity toward various types of collagen and toward the serum mannose-binding lectin MBL, which contains a collagen domain. Amino acid analysis of the products of GLT25D1 and GLT25D2 reactions confirmed the transfer of galactose to hydroxyly... | SIGNOR-261155 |
P05771 | Q06187 | 1 | phosphorylation | down-regulates activity | 0.382 | We provide direct evidence that PKCbeta acts as a feedback loop inhibitor of Btk activation. Inhibition of PKCbeta results in a dramatic increase in B-cell receptor (BCR)-mediated Ca2+ signaling. We identified a highly conserved PKCbeta serine phosphorylation site in a short linker within the Tec homology domain of Btk... | SIGNOR-249110 |
Q9Y696 | Q00535 | 0 | phosphorylation | up-regulates quantity by stabilization | 0.2 | These results confirm that CDK5 phosphorylates CLIC4 at serine 108.|We found that activated CDK5 phosphorylated serine 108 in CLIC4, increasing CLIC4 protein stability, and accumulation. | SIGNOR-279451 |
P06493 | O75122 | 1 | phosphorylation | up-regulates activity | 0.569 | Overall, these results support the idea that phosphorylation of CLASP2 on S1234 by Cdk1, but not phosphorylation of the CLASP2 C terminal by Plk1, is required to maintain mitotic spindle bipolarity.|We propose that Cdk1 and Plk1 mediate a CLASP2 phospho-switch that is necessary to stabilize KT-MT attachments in human c... | SIGNOR-278233 |
Q7Z2W4 | Q14258 | 0 | ubiquitination | up-regulates activity | 0.398 | Our data demonstrates that TRIM25 triggers ubiquitination of ZAP and enhances its antiviral activity through inhibition of viral translation, highlighting the importance of cofactors in the mechanisms of broadly antiviral proteins. | SIGNOR-278565 |
O60346 | Q13043 | 1 | dephosphorylation | up-regulates activity | 0.295 | PHLPPs dephosphorylate Mst1 on the T387 inhibitory site, which activate Mst1 and its downstream effectors p38 and JNK to induce apoptosis. | SIGNOR-248329 |
P60953 | O15068 | 0 | guanine nucleotide exchange factor | up-regulates activity | 0.751 | We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity... | SIGNOR-260560 |
O95071 | Q9BPZ3 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.445 | We demonstrate a mechanism for this co-regulation that involves an E3 ubiquitin ligase, EDD, which targets Paip2 for degradation. PABP depletion by RNA interference (RNAi) causes co-depletion of Paip2 protein without affecting Paip2 mRNA levels. Upon PABP knockdown, Paip2 interacts with EDD, which leads to Paip2 ubiqui... | SIGNOR-272648 |
Q9NRM7 | Q9GZV5 | 1 | phosphorylation | down-regulates | 0.698 | Activated lats1/2 in turn phosphorylate and inhibit yap/taz transcription co-activators | SIGNOR-175787 |
O00429 | P42345 | 0 | phosphorylation | up-regulates activity | 0.345 | Furthermore, we confirmed also in Jurkat cells that the specific silencing of both ERK1/2 and mTOR by siRNA downregulates Drp1 phosphorylation on Ser616 | SIGNOR-275430 |
Q13118 | P04049 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.2 | RAF1 phosphorylates the Thr93 site of KLF10 in vivo. Since the phosphorylation of Thr93 enables KLF10 and PIN1 to bind, it seems likely that RAF-1 will have an effect on KLF10 stability that is similar to that of PIN1.PIN1 facilitates KLF10 protein degradation. ( | SIGNOR-276502 |
Q13976 | P49841 | 1 | phosphorylation | down-regulates activity | 0.26 | Moreover, PrkG1 inhibits GSK3\u03b2 by binding and directly phosphorylating GSK3\u03b2 at its serine-9 residue (Zhao et al., xref ).|Moreover, PrkG1 inhibits GSK3beta by binding and directly phosphorylating GSK3beta at its serine 9 residue. | SIGNOR-280094 |
O95644 | P53779 | 0 | phosphorylation | down-regulates | 0.445 | We show that jnk, erk, and p38 physically associate with the nfatc n-terminal regulatory domain and can directly phosphorylate functionally important residues involved in regulating nfatc subcellular localization, namely ser(172) and the conserved nfatc ser-pro repeats. | SIGNOR-74556 |
Q9UMS4 | O43395 | 1 | polyubiquitination | up-regulates activity | 0.754 | Here, we report that the spliceosomal Prp19 complex modifies Prp3, a component of the U4 snRNP, with nonproteolytic K63-linked ubiquitin chains. The K63-linked chains increase the affinity of Prp3 for the U5 snRNP component Prp8, thereby allowing for the stabilization of the U4/U6.U5 snRNP. | SIGNOR-271966 |
P15514 | P19544 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.396 | The Wilms Tumor Suppressor WT1 Encodes a Transcriptional Activator of amphiregulin | SIGNOR-251745 |
P22607 | P40763 | 1 | phosphorylation | up-regulates activity | 0.631 | Activation of Stat1 and Stat3 by FGFR derivatives. Lysates of 293T cells transfected as indicated were analysed by Western blotting using Phospho-Stat1 (Y701) antisera (top) or Stat1 antisera (bottom). (b) The same lysates in (a) were re-examined for phosphorylated Stat3 by Western blotting with Phospho-Stat3 (Y705) (t... | SIGNOR-251139 |
P51452 | P29597 | 0 | phosphorylation | up-regulates | 0.265 | Phosphorylation of vhr at tyr(138) was required for its phosphatase activity toward stat5. In addition, the src homology 2 domain of stat5 was required for the effective dephosphorylation of stat5 by vhr. The tyrosine kinase tyk2, which mediates the phosphorylation of stat5, was also responsible for the phosphorylation... | SIGNOR-157655 |
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