IdA string | IdB string | labels int64 | mechanism string | effect string | score float64 | sentence string | signor_id string |
|---|---|---|---|---|---|---|---|
P10451 | Q13950 | 0 | transcriptional regulation | up-regulates quantity | 0.488 | Ets-1 and Runx2 are critical transcriptional regulators of OPN expression in CT26 colorectal cancer cells. Suppression of these transcription factors results in significant down-regulation of the OPN metastasis protein. | SIGNOR-245336 |
P43403 | P06241 | 0 | phosphorylation | up-regulates activity | 0.574 | Subsequently, Lck and Fyn phosphorylate and activate the Syk family kinase ZAP-70 when it is recruited to the phosphorylated ITAM motifs xref . | SIGNOR-279043 |
P52292 | Q09472 | 0 | acetylation | up-regulates | 0.357 | Ampk triggered the acetylation of importin alpha1 on lys(22), a process dependent on the acetylase activity of p300 | SIGNOR-128625 |
O14733 | Q13233 | 0 | phosphorylation | up-regulates | 0.723 | Here we show that jnkk2, a novel member of the map kinase kinase family, was phosphorylated and activated by mekk1 | SIGNOR-51207 |
Q9UKX5 | Q02447 | 0 | null | up-regulates quantity by expression | 0.2 | We speculate that the "mesenchymal signature" of alpha11 integrin gene expression is controlled by the activity of Sp1/Sp3, fibroblast-specific combinations of Ets family members and yet unidentified enhancer-binding transcription factors. | SIGNOR-253351 |
O14770 | P42771 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.2 | We show that the Pbx1 and Meis2 homeodomain proteins interact with Klf4 and can be recruited to DNA elements comprising a Klf4 site or G C box, with adjacent Meis and Pbx sites. Meis2d and Pbx1a activate expression of p15(Ink4a) and E-cadherin, dependent on the Meis2d transcriptional activation domain. We suggest a mod... | SIGNOR-267240 |
P19525 | Q9NYA1 | 1 | phosphorylation | up-regulates activity | 0.2 | This suggests that PKR is critical in the phosphorylation of SPHK1 at Ser225.|We confirmed that phosphorylated PKR activates SPHK1 kinase activity, but it remained necessary to determine whether there has mutual correlation or any reciprocal effect between these two kinases in stressed cells. | SIGNOR-278514 |
Q5S007 | P61026 | 1 | phosphorylation | down-regulates activity | 0.33 | To investigate whether the phosphorylation of Rab10 by LRRK2 is direct, we performed an in vitro kinase assay using recombinant components. Notably, we found that both wt and LRRK2-G2019S, but neither kinase inactive D1994A mutant nor small molecule-inhibited LRRK2, efficiently phosphorylated Rab10, proving a direct ki... | SIGNOR-261277 |
P53778 | Q13424 | 1 | phosphorylation | up-regulates | 0.668 | Sapk3 phosphorylates alpha1-syntrophin at serine residues 193 and 201 in vitro and phosphorylation is dependent on binding to the pdz domain of alpha1-syntrophin. The finding that sapk3 co-localizes with _1-syntrophin in skeletal muscle, that it binds to the pdz domain of _1-syntrophin, and that phosphorylation of _1-s... | SIGNOR-67061 |
P50613 | Q9NYV4 | 1 | phosphorylation | up-regulates activity | 0.51 | Although Cdk12/CycK kinase complex lacking T-loop phosphorylation showed some basal activity towards a CTD substrate prephosphorylated at position Ser7, its activity was significantly increased upon coexpression with the CAK from S. cerevisiae (Supplementary Fig. 9a). Mutation of T893 to E to mimic phosphorylation show... | SIGNOR-275509 |
P10644 | P24941 | 0 | phosphorylation | up-regulates | 0.343 | In this context, we have identified rialpha as a novel substrate for the g(1)/s-cyclin-dependent kinase, cdk2/cyclin e, and found that rialpha is specifically phosphorylated at the serine residue. | SIGNOR-145577 |
B2RTY4 | P61586 | 1 | gtpase-activating protein | down-regulates activity | 0.565 | We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity... | SIGNOR-260508 |
P00540 | Q02750 | 1 | phosphorylation | up-regulates activity | 0.485 | Our data indicate that Mos activated MEK1 in vitro as well as in vivo by phosphorylating Ser 222. | SIGNOR-260920 |
Q96J02 | Q7Z434 | 1 | ubiquitination | down-regulates quantity by destabilization | 0.646 | These data collectively indicate that AIP4 is the E3 ligase for MAVS.|We generated single substitutions (K362A, K371A or K420A) and combined point substitutions of MAVS and tested their degradation. K371A or K420A MAVS showed partial resistance to PCBP2-induced degradation (data not shown), whereas MAVS with the combin... | SIGNOR-260362 |
Q96DX5 | P12277 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.683 | We demonstrate that creatine kinase B (CKB) interacts with Asb-9 in a specific, SOCS box-independent manner. This interaction increases the polyubiquitylation of CKB and decreases total CKB levels within the cell. The targeting of CKB for degradation by Asb-9 was primarily SOCS box-dependent and suggests that Asb-9 act... | SIGNOR-271623 |
Q9UKX2 | Q14814 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.327 | Myocyte enhancer factor-2 and serum response factor binding elements regulate fast Myosin heavy chain transcription in vivo. We show that the upstream promoter region of the gene most abundantly expressed in mouse skeletal muscles, IIb MyHC, retains binding activity and transcriptional activation for three positive tra... | SIGNOR-238712 |
O14757 | P12830 | 1 | phosphorylation | down-regulates activity | 0.301 | Phosphorylation of Cdh1 by Chk1 promotes recognition of Cdh1 by SCF betaTRCP1.|These data suggest that Chk1 negatively regulates APC/C Cdh1 activity by both promoting Cdh1 destruction and by destabilizing its association with the APC/C. | SIGNOR-278396 |
P36897 | P29353 | 1 | phosphorylation | up-regulates | 0.548 | We now report that upon TGF-_ stimulation, T_RI phosphorylates ShcA on serine and, to a lesser degree, on tyrosine to activate Erk MAP kinases. | SIGNOR-227503 |
P29350 | P32927 | 1 | dephosphorylation | down-regulates | 0.522 | However, inhibition of shp2 binding to betac, did not prevent tyrosine phosphorylation of shp2. Interestingly, this same phosphopeptide served as a substrate for the tyrosine phosphatase activity of both shp1 and shp2. | SIGNOR-114597 |
O00743 | Q8N884 | 1 | dephosphorylation | down-regulates activity | 0.2 | In this study, we found that PPP6C suppressed phosphorylation of human cGAS (hcGAS) at S435 or mouse cGAS (mcGAS) at S420 in its substrate-binding pocket, thus preventing its binding to GTP and inhibiting the synthesis of cGAMP.|These data suggest that PPP6C inhibits cGAS activity. | SIGNOR-276990 |
P12931 | O15524 | 1 | phosphorylation | down-regulates activity | 0.45 | SOCS1 is phosphorylated on Y80 by SRC family kinase members SRC and YES1. | SIGNOR-276857 |
P46937 | P31749 | 0 | phosphorylation | down-regulates | 0.593 | One protein that associates with 14-3-3 in an akt-dependent manner is shown here to be the yes-associated protein (yap), which is phosphorylated by akt at serine 127, leading to binding to 14-3-3. Akt promotes yap localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of t... | SIGNOR-252593 |
P31645 | P12931 | 0 | phosphorylation | up-regulates quantity by stabilization | 0.258 | We found that 1) SERT exists in a tyrosine-phosphorylated form, 2) inhibition of tyrosine kinase(s) reduces SERT expression levels by facilitating SERT protein degradation, 3) Src-kinase activity up-regulates SERT protein expression with a concomitant increase in 5-HT uptake and tyrosine phosphorylation, and 4) mutatio... | SIGNOR-276386 |
P50549 | Q15418 | 0 | phosphorylation | up-regulates activity | 0.35 | Here we describe that the 90-kDa ribosomal S6 kinase 1 (RSK1), a protein kinase downstream of the extracellular signal-regulated kinase (ERK) subclass of MAPKs, binds to ER81, phosphorylates it, and enhances ER81-dependent transcription. Two in vivo RSK1 phosphorylation sites within ER81, Ser(191) and Ser(216), were id... | SIGNOR-249163 |
O95398 | P62834 | 1 | guanine nucleotide exchange factor | up-regulates activity | 0.71 | Epac1 (cAMP-GEFI) and Epac2 (cAMP-GEFII) are closely related guanine nucleotide exchange factors (GEFs) for the small GTPase Rap1, which are directly regulated by cAMP. Here we show that both GEFs efficiently activate Rap2 as well. | SIGNOR-263956 |
O60936 | P68400 | 0 | phosphorylation | up-regulates activity | 0.324 | Phosphorylation of ARC at T149 Is Required for Its Antiapoptotic Effect. Here we report that the function of ARC is regulated by protein kinase CK2. ARC at threonine 149 is phosphorylated by CK2. This phosphorylation targets ARC to mitochondria. ARC is able to bind to caspase-8 only when it is localized to mitochondria... | SIGNOR-262837 |
P31749 | Q9BRS8 | 1 | phosphorylation | up-regulates activity | 0.353 | Akt dependent phosphorylation of LARP6. We provide the first description that LARP6 is phosphorylated at multiple sites and that phosphorylation of S451 is critical to activate the protein in type I collagen biosynthesis. | SIGNOR-277213 |
P00519 | Q99638 | 1 | phosphorylation | up-regulates activity | 0.477 | The SH3 domain of c-Abl interacts directly with the C-terminal region of Rad9. c-Abl phosphorylates the Rad9 Bcl-2 homology 3 domain (Tyr-28) in vitro and in cells exposed to DNA-damaging agents. | c-Abl-mediated phosphorylation of Rad9 induces binding of Rad9 to Bcl-xL |these findings indicate that Rad9 is regulated b... | SIGNOR-260843 |
Q13443 | P50281 | 0 | cleavage | down-regulates quantity by destabilization | 0.341 | Here we show that MT1-MMP forms a complex with FGFR2 and ADAM9 in osteoblasts and proteolytically inactivates ADAM9|Western blotting using antibodies against ectodomain of ADAM9 detected a fragment (around 26 kDa) of ADAM9 in the conditioned culture medium from cells cotransfected with wild-type MT1-MMP, but not in tha... | SIGNOR-260301 |
P04637 | P06493 | 0 | phosphorylation | up-regulates activity | 0.579 | The N-terminus of E2F1 can interact directly with a region towards the C-terminus of p53, resulting in increased nuclear retention of p53 and p53-mediated transcription and apoptosis. This is inhibited by competition between p53 and cyclin A at the binding site within E2F19,10. The interaction between p53 and E2F1 is e... | SIGNOR-167779 |
P05771 | Q14847 | 1 | phosphorylation | down-regulates activity | 0.2 | Actin binding of human lim and sh3 protein is regulated by cgmp- and camp-dependent protein kinase phosphorylation on serine 146. Phosphorylation of lasp at ser-146 leads to a redistribution of the actin-bound protein from the tips of the cell membrane to the cytosol, accompanied with a reduced cell migration | SIGNOR-97942 |
P35222 | Q96EB6 | 0 | deacetylation | up-regulates quantity | 0.505 | SIRT1 deacetylates β-catenin to promote its accumulation in the nucleus and thus induces the transcription of genes that block MSC adipogenesis. | SIGNOR-256208 |
Q6R6M4 | O14503 | 1 | deubiquitination | up-regulates quantity by stabilization | 0.2 | Upon DNA damage, DEC1 is rapidly induced in an ATM/ATR-dependent manner. DEC1 induction results from protein stabilization via a mechanism that requires the USP17 ubiquitin protease. USP17 binds and deubiquitylates DEC1, markedly extending its half-life. | SIGNOR-276852 |
O96013 | P07954 | 1 | phosphorylation | down-regulates quantity | 0.2 | FH is massively phosphorylated at the Ser 46 by PAK4 in non-small cell lung cancer (NSCLC) cells, and PAK4-phosphorylated FH binds to 14-3-3, resulting in cytosolic detention of FH and prohibition of FH/CSL/p53 complex formation. | SIGNOR-266315 |
P11021 | Q15011 | 0 | relocalization | up-regulates quantity by stabilization | 0.411 | A key inhibitor of the turnover and Nt-arginylation of BiP was HERP (homocysteine-responsive ER protein), a 43-kDa ER membrane-integrated protein that is an essential component of ER-associated protein degradation. | SIGNOR-261346 |
Q13557 | Q14654 | 1 | phosphorylation | down-regulates | 0.2 | Results showed that activation of camkii triggered dynamin-dependent internalization of k(atp) channels. This process required phosphorylation of threonine at 180 and 224 and an intact (330)yskf(333) endocytosis motif of the k(atp) channel kir6.2 pore-forming subunit. | SIGNOR-200027 |
P42345 | Q9HBH9 | 1 | phosphorylation | down-regulates activity | 0.274 | MTOR phosphorylates MNK2a on Ser74. Here, we show that mTORC1, a key regulator of mRNA translation and oncogenesis, directly phosphorylates MNK2 on Ser74. This suppresses MNK2 activity and impairs binding of MNK2 to eIF4G. | SIGNOR-277516 |
O00273 | P42574 | 0 | cleavage | up-regulates activity | 0.755 | DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. We have identified and purified from HeLa cytosol a protein that induces DNA fragmentation in coincubated nuclei after it is activated by caspase-3. | SIGNOR-47416 |
P10586 | P07949 | 1 | dephosphorylation | down-regulates | 0.426 | Lar expression significantly reduced tyrosine-1062 phosphorylation in ret-men2a but not in ret-men2b | SIGNOR-85170 |
Q16665 | P13674 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.314 | Hypoxia upregulates P4HA1 expression in PDAC PDOs through HIF1α. Our results show that the treatment of PDO4 and PDO11 cells with 10 μmol/L of PX-478 for 24 hours reduced the levels of P4HA1 in hypoxia (Fig. 2F), suggesting P4HA1 expression in hypoxia is regulated by HIF1α expression. | SIGNOR-279840 |
P17252 | P29972 | 1 | phosphorylation | up-regulates | 0.2 | Activation of protein kinase c (pkc) by 1-oleoyl-2-acetyl-sn-glycerol (oag) induced a marked increase of aqp1-dependent water permeability. This regulation was abolished in mutated aqp1 channels lacking both consensus pkc phosphorylation sites thr(157) and thr(239) (termed aqp1 deltapkc). | SIGNOR-155106 |
P55075 | Q05925 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.452 | Our results in ES cells suggest that Engrailed inhibits Fgf8 expression in the absence of Pbx1. We identified single Engrailed- and Pbx-binding sites in the Fgf8 intron that inhibit expression of Fgf8 in mouse ES cells, but that together can allow full Fgf8 expression. Our data support the model that Engrailed heterodi... | SIGNOR-265776 |
P35869 | P49841 | 0 | phosphorylation | up-regulates activity | 0.25 | A proposed model of GSK3β role on AHR function and degradation. AHR is phosphorylated by GSK3β in a p23-dependent manner in HeLa cells. This phosphorylation is required for optimal activation of the ligand-dependent AHR target gene transcription. After phosphorylation, AHR is K63-ubiquitinated and is targeted for the L... | SIGNOR-276664 |
P26651 | P15172 | 1 | post transcriptional regulation | down-regulates quantity by destabilization | 0.292 | The TTP binding site in the 3′ UTR of MyoD would permit TTP-mediated mRNA decay | SIGNOR-253597 |
Q9Y2W1 | P21127 | 0 | phosphorylation | up-regulates activity | 0.206 | In addition, genetic knockout of CLK1 or chemical inhibition in mice ameliorated diet-induced obesity and insulin resistance at 22\u00b0C. Through proteomics, we uncovered thyroid hormone receptor-associated protein 3 (THRAP3) as an interacting partner of CLK1, further confirmed by co-immunoprecipitation assays.|We fur... | SIGNOR-280209 |
Q13144 | P41091 | 1 | guanine nucleotide exchange factor | up-regulates activity | 0.735 | EIF2B converts the protein synthesis initiation factor 2 (eIF2) from an inactive GDP-bound form to an active eIF2-GTP complex owing to its guanine nucleotide exchange factor (GEF) activity. | SIGNOR-269133 |
P11388 | P68400 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.595 | This study also reports the novel finding that topoIIα may be a target of GSK3β phosphorylation. Evidence suggests that CK2 serves as a priming kinase, through phosphorylation at Ser1365, for GSK3β-mediated phosphorylation at Ser1361. | SIGNOR-276300 |
P61289 | O96017 | 0 | phosphorylation | up-regulates activity | 0.349 | REGγ interacts with DBC1 and is phosphorylated by Chk2. | SIGNOR-273611 |
O60674 | Q06187 | 1 | phosphorylation | up-regulates activity | 0.447 | Jak2 and Lyn coimmunoprecipitated with Btk and phosphorylated Btk on tyrosine (XREF_FIG C). | SIGNOR-279196 |
Q00987 | P46531 | 1 | ubiquitination | up-regulates | 0.476 | Although the interaction between notch1 and mdm2 results in ubiquitination of notch1, this does not result in degradation of notch1, but instead leads to activation of the intracellular domain of notch1. | SIGNOR-200197 |
O15169 | Q9NTX7 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.645 | Here, we identify RNF146, a RING-domain E3 ubiquitin ligase, as a positive regulator of Wnt signalling. RNF146 promotes Wnt signalling by mediating tankyrase-dependent degradation of axin. Mechanistically, RNF146 directly interacts with poly(ADP-ribose) through its WWE domain, and promotes degradation of PARsylated pro... | SIGNOR-263335 |
Q6PIY7 | P31749 | 0 | phosphorylation | down-regulates activity | 0.2 | We found that Gld2 activity is regulated by site-specific phosphorylation in its disordered N-terminal domain. We identified two phosphorylation sites (S62, S110) where phosphomimetic substitutions increased Gld2 activity and one site (S116) that markedly reduced activity. Using mass spectrometry, we confirmed that HEK... | SIGNOR-259405 |
Q5S007 | P61006 | 1 | phosphorylation | up-regulates activity | 0.33 | In a screen for Rab8A kinases we identify TAK1 and MST3 kinases that can efficiently phosphorylate the Switch II residue Threonine72 (Thr72) in a similar manner as LRRK2 in vitro. |Overall our data suggests that the phosphorylation of Rab8A at Ser111 may influence Switch II-binding by regulators, thus disrupting intera... | SIGNOR-260267 |
O60260 | P02788 | 1 | ubiquitination | down-regulates activity | 0.2 | We propose that Parkin ubiquitylation of LTF at K649 perturbs LTF\u2019s ability to accumulate intracellular iron levels and that depletion of Parkin, or substitution of K649 on LTF, allows LTF to accumulate intracellular iron levels.|Parkin dependent ubiquitylation of LTF occurred most often on lysines (K) 182 and 649... | SIGNOR-278641 |
Q7Z6Z7 | O95251 | 1 | ubiquitination | down-regulates quantity by destabilization | 0.2 | Moreover, we determined that Huwe1 is a novel E3 ligase for Myst2 degradation in mESCs, and Brpf3 disturbs Huwe1 mediated ubiquitination of Myst2 via interaction with Huwe1 and Myst2. | SIGNOR-278652 |
Q12968 | P45983 | 0 | phosphorylation | down-regulates | 0.837 | Jnks directly phosphorylate nuclear factor of activated t-cell (nfat) transcription factors, thus antagonizing the effects of calcium-regulated signaling through the protein phosphatase calcineurin | SIGNOR-118220 |
Q9C037 | O75298 | 1 | ubiquitination | down-regulates quantity | 0.2 | 2: TRIM4 ubiquitinates and degrades the NSP2 protein.|Mechanistic studies showed that TRIM4 ubiquitinated modified NSP2 and down-regulated NSP2 expression. | SIGNOR-278793 |
P35813 | Q86WV6 | 1 | dephosphorylation | down-regulates activity | 0.2 | Collectively, our findings suggest that the overexpression of PPM1A antagonizes the STING- and TBK1-induced type I interferon signaling pathway.|First, PPM1A directly dephosphorylated STING, likely via its S358 site (Figs.|Moreover, our study demonstrates that while TBK1 enhances STING aggregation in a kinase activity-... | SIGNOR-276958 |
Q5XPI4 | P45973 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.2 | In the present study, we report that HP1α and β undergo proteasomal degradation in lamin A/C knock-down cells and show by ectopic expression, RNAi and binding studies that the RING finger ubiquitin ligase RNF123 is directly involved in HP1 degradation. | SIGNOR-272035 |
P04637 | Q14694 | 0 | deubiquitination | up-regulates quantity by stabilization | 0.662 | Since USP10 is known as a deubiquitinating protease of p53 (Yuan et al., 2010), inhibition of USP10 by spautin-1 may promote the degradation of p53. | SIGNOR-260297 |
O96017 | P10636 | 1 | phosphorylation | down-regulates | 0.2 | Tau pseudophosphorylation at specific sites such as s262, s293, s324 and s356 was reported to induce tau conformational change and attenuate tau binding to microtubules (fischer et al., 2009). Then, newly soluble tau proteins are targeted by post-translational modifications that directly or indirectly alter tau conform... | SIGNOR-171026 |
P67775 | Q04206 | 1 | dephosphorylation | down-regulates | 0.483 | Rela was dephosphorylated by a purified pp2a core enzyme, a heterodimer formed by the catalytic subunit of pp2a (pp2ac) and pr65, in a concentration-dependent manner.These data suggest that the constitutive activation of rela in melanoma cells could be due, at least in part, to the deficiency of pp2a, which exhibits de... | SIGNOR-110959 |
P52926 | P06493 | 0 | phosphorylation | down-regulates | 0.374 | Architecture of high mobility group protein i-c dna complex and its perturbation upon phosphorylation by cdc2 kinase. Phosphorylation by cdc2 reduces binding strength of the mammalian and insect hmgi proteins to dna. After phosphorylation of the protein at ser-43 and ser-58 by cdc2 kinase multiple contacts of dbds, esp... | SIGNOR-74098 |
O15264 | Q12888 | 1 | phosphorylation | down-regulates activity | 0.2 | Here we show that 53BP1 is phosphorylated during mitosis on two residues, T1609 and S1618, located in its well-conserved ubiquitination-dependent recruitment (UDR) motif.|Dephosphorylation enables the recruitment of 53BP1 to double-strand DNA breaks |phosphorylation of T1609 is likely to be mediated by p38 MAPK | SIGNOR-264449 |
P28482 | O60674 | 1 | phosphorylation | down-regulates | 0.513 | We hypothesize that phosphorylation of ser523 in jak2 by erks 1 and/or 2 or other as-yet-unidentified kinases acts in a negative feedback manner | SIGNOR-236331 |
Q13882 | Q12929 | 1 | phosphorylation | up-regulates activity | 0.357 | Eps8 which was identified by this method is phosphorylated by Myr-PTK6 in HEK293 cells. Mouse Eps8 expressed in HEK293 cells is phosphorylated by Myr-PTK6 at residues Tyr497, Tyr524, and Tyr534. These results indicate that plasma-membrane-associated PTK6 phosphorylates Eps8, which promotes cell proliferation, adhesion... | SIGNOR-263191 |
P06239 | P22681 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.711 | Coexpression in 293T cells demonstrated that Lck kinase activity and Cbl ubiquitin ligase activity were essential for Lck ubiquitination and negative regulation of Lck-dependent serum response element-luciferase reporter activity. The Lck SH3 domain was pivotal for Cbl-Lck association and Cbl-mediated Lck degradation, ... | SIGNOR-272614 |
Q15746 | P00519 | 0 | phosphorylation | up-regulates | 0.3 | Nonmuscle myosin light chain kinase (nmmlck), a multi-functional cytoskeletal protein critical to vascular homeostasis, is highly regulated by tyrosine phosphorylation. We identified multiple novel c-abl-mediated nmmlck phosphorylation sites by mass spectroscopy analysis (including y231, y464, y556, y846) and examined ... | SIGNOR-167989 |
P20336 | P53611 | 0 | lipidation | up-regulates activity | 0.656 | Prenylation (or geranylgeranylation) of Rab GTPases is catalysed by RGGT (Rab geranylgeranyl transferase) and requires REP (Rab escort protein). In the classical pathway, REP associates first with unprenylated Rab, which is then prenylated by RGGT. In the alternative pathway, REP associates first with RGGT; this compl... | SIGNOR-265575 |
P17612 | Q9Y6D6 | 1 | phosphorylation | up-regulates activity | 0.2 | Within 20 min after addition of 8-Br-cAMP, BIG1 accumulated in nuclei, and this effect was blocked by protein kinase A (PKA) inhibitors H-89 and PKI, suggesting a dependence on PKA-catalyzed phosphorylation. |Mutant BIG1 (S883A) in which Ala replaced Ser-883, a putative PKA phosphorylation site, did not move to the nuc... | SIGNOR-272146 |
P35968 | P48730 | 0 | phosphorylation | down-regulates activity | 0.328 | CKIdelta phosphorylates VEGFR2 at both DSG and DDTD phosphodegrons to promote its interaction with beta-TRCP1.|In a reciprocal set of experiments, we found that overexpression of CKIdelta markedly decreased the half-life of VEGFR2 (XREF_FIG). | SIGNOR-280235 |
P27216 | P46934 | 1 | relocalization | up-regulates quantity | 0.344 | Annexin XIII has two known isoforms, a and b, that are apically localized, although XIIIa is also found in the basolateral compartment. In vitro binding and coprecipitation experiments showed that the Nedd4-C2 domain interacts with both annexin XIIIa and b in the presence of Ca2+, and the interaction is direct and opti... | SIGNOR-272571 |
Q99683 | Q5SGD2 | 0 | dephosphorylation | down-regulates | 0.318 | Exogenous pp2cepsilon associated with exogenous ask1 in hek-293 cells under non-stressed conditions, inactivating ask1 by decreasing thr845 phosphorylation | SIGNOR-154554 |
P49137 | Q16539 | 0 | phosphorylation | up-regulates activity | 0.769 | Here we show that in vitro rk phosphorylates human gst-mapkap kinase-2 at thr25 in the proline-rich n-terminal region thr222 and ser272 in the catalytic domain and thr334 in the c-terminal domain. Using novel methodology we demonstrate that activation of mapkap kinase-2 requires the phosphorylation | SIGNOR-44351 |
O94953 | P04637 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.281 | KDM4B/JMJD2B is a p53 target gene that modulates the amplitude of p53 response after DNA damage. p53 directly regulates JMJD2B gene expression by binding to a canonical p53-consensus motif in the JMJD2B promoter. | SIGNOR-263729 |
P35813 | Q16539 | 1 | dephosphorylation | down-regulates activity | 0.408 | Moreover, when expressed in mammalian cells, pp2ca inhibited the activation of the p38 and jnk cascades induced by environmental stresses. Both in vivo and in vitro observations indicated that pp2ca dephosphorylated and inactivated mapkks (mkk6 and sek1) and a mapk (p38) in the stress-responsive mapk cascades. Furtherm... | SIGNOR-59618 |
Q13153 | P27361 | 0 | phosphorylation | down-regulates | 0.337 | Activated erk can phosphorylate t292 in the prs, and this blocks the ability of pak to phosphorylate s298 and of rac-pak signaling to enhance mek1-erk complex formation. | SIGNOR-123074 |
Q14653 | Q12899 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.672 | TRIM26 bound to IRF3 and promoted its K48-linked polyubiquitination and degradation in nucleus. | SIGNOR-272440 |
P17302 | P05129 | 0 | phosphorylation | down-regulates activity | 0.568 | Phosphorylation of connexin43 on serine368 by protein kinase C regulates gap junctional communication.|These data strongly suggest that PKC directly phosphorylates Cx43 on S368 in vivo, which results in a change in single channel behavior that contributes to a decrease in intercellular communication. | SIGNOR-249050 |
P46531 | Q8NBL1 | 0 | glycosylation | up-regulates | 0.606 | O-glucosylation of epidermal growth factor-like (egf) repeats in the extracellular domain of notch is essential for notch function. A udp-glucose:protein o-glucosyltransferase (poglut/rumi) transfers o-glucose to serine within the o-glucose consensus. | SIGNOR-198713 |
P12931 | Q13642 | 1 | phosphorylation | up-regulates activity | 0.26 | However, overexpression of Src promoted most of the Flag-FHL1-WT to translocate to the nucleus, whereas the Flag-FHL1-Y149-272F mutant (phosphorylation deficient mutant) remained in the cytoplasm (XREF_FIG).|We found that Src phosphorylates FHL1 at Y149 and Y272, demonstrating that FHL1 is a bona fide novel substrate o... | SIGNOR-278213 |
Q14191 | P78527 | 0 | phosphorylation | up-regulates | 0.641 | Here, we identify ser-440 and -467 in wrn as major phosphorylation sites mediated by dna-pk our findings indicate that phosphorylation of ser-440 and -467 in wrn are important for relocalization of wrn to nucleoli, and that it is required for efficient dsb repair. | SIGNOR-203737 |
P28482 | P50548 | 1 | phosphorylation | up-regulates | 0.596 | The experiments presented here indicate that erf is regulated during nuclear import and/or export and that this process depends on its phosphorylation by erks our analysis indicates that in addition to t526 (position 7), s161 (position 2), s246 (position 3), and s251 (position 4) are also phosphorylated in vitro by er... | SIGNOR-67524 |
O96017 | Q13972 | 1 | phosphorylation | down-regulates | 0.405 | During interphase, cdc25 is inhibited by ser287 phosphorylation (xenopus cdc25;ser 216 in human cdc25c) and this inhibitory phosphorylation is maintained by dna-responsive checkpoints / s287 is targeted by many kinases, including chk1, chk2, ctak-1, pka, p38 and mapkap kinase-2 suggesting that phosphorylation of this s... | SIGNOR-150843 |
P01189-PRO_0000024969 | P29120 | 0 | cleavage | up-regulates quantity | 0.2 | POMC is post-translationally cleaved by prohormone convertase enzymes 1 and 2 (PC1, PC2) into ACTH, an N-terminal glycopeptide | SIGNOR-268724 |
P15172 | Q12857 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.246 | NFIA binds to and activates the brown-fat-specific enhancers even before differentiation and later facilitates the binding of PPARgamma|NFIA has at least three functions on the transcriptional regulation of brown fat [2]. First, NFIA activates adipogenesis per se, through activating the transcription of Pparg, which en... | SIGNOR-263982 |
Q9ULT6 | O60353 | 1 | ubiquitination | down-regulates quantity | 0.614 | Here we show that the cell-surface transmembrane E3 ubiquitin ligase zinc and ring finger 3 (ZNRF3) and its homologue ring finger 43 (RNF43) are negative feedback regulators of Wnt signalling. ZNRF3 is associated with the Wnt receptor complex, and inhibits Wnt signalling by promoting the turnover of frizzled and LRP6. | SIGNOR-260114 |
Q13541 | P49841 | 0 | phosphorylation | down-regulates activity | 0.364 | We found that gsk-3Beta phosphorylates and inactivates 4e-bp1, thereby increasing eif4e-dependent protein synthesis. upon stimulation, 4e-bp1 is phosphorylated on several threonine and serine residues, including thr-37/46 (36). This results in dissolution of the complex, freeing eif4e to bind with mrna cap to promote t... | SIGNOR-236026 |
Q9ULU4 | P30542 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.2 | We also confirmed transcriptional coactivator functions of ZMYND8 in ERα-driven reporter assays and on endogenous E2-dependent genes (Figure 5F,G). siRNA knockdown of ZMYND8 showed markedly decreased transcription at the presumptive ERα/Z3 target genes ADORA1 and NAV2, while the classical ERα targets pS2/TFF1 and GREB1... | SIGNOR-266208 |
Q92831 | Q16695 | 1 | acetylation | down-regulates activity | 0.2 | The HAT module within the SAGA and ADA complexes acetylates histone H3, mainly on residues K9 and K14. | SIGNOR-269621 |
Q8TCQ1 | P13762 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.2 | Two E3 ligases, MARCH I and MARCH VIII, have been shown to polyubiquitinate lysine residue 225 in the cytoplasmic tail of I-Abeta and HLA-DRbeta. We show that lysine residue 219 in the cytoplasmic tail of DRalpha is also subject to polyubiquitination. | SIGNOR-271409 |
P35813 | Q14164 | 1 | dephosphorylation | down-regulates activity | 0.279 | PPM1A directly dephosphorylates both MAVS and TBK1 and IKKepsilon.|In a similar in vitro phosphatase assay, incubation of PPM1A also eliminated TBK1 and IKKepsilon phosphorylation at Ser 172 residue, evidenced by phospho-S172 immunoblotting (XREF_FIG, F and G).|These observations suggest that PPM1A may block kinase act... | SIGNOR-277072 |
Q14940 | P32121 | 0 | relocalization | down-regulates activity | 0.397 | Internalization of the Na(+)/H(+) exchanger NHE5 into recycling endosomes is enhanced by the endocytic adaptor proteins beta-arrestin1 and -2, best known for their preferential recognition of ligand-activated G protein-coupled receptors (GPCRs) | SIGNOR-275506 |
P45974 | Q08050 | 1 | deubiquitination | up-regulates quantity by stabilization | 0.351 | Wnt signaling activation inhibits FoxM1 phosphorylation by GSK3-Axin complex and leads to interaction between FoxM1 and deubiquitinating enzyme USP5, thereby deubiquitination and stabilization of FoxM1. | SIGNOR-277210 |
P09211 | Q05513 | 0 | phosphorylation | up-regulates activity | 0.2 | Peptide phosphorylation analyses and both phosphorylation and enzyme kinetic studies with GSTP1 proteins mutated at candidate amino acid residues established Ser-42 and Ser-184 as putative phospho-acceptor residues for both kinases in the GSTP1 protein. Together, these findings show PKA- and PKC-dependent phosphorylati... | SIGNOR-276017 |
Q9Y297 | Q13315 | 0 | phosphorylation | up-regulates quantity by stabilization | 0.299 | ATM phosphorylates and stabilizes β-TrCP1 upon DNA damage. | SIGNOR-277549 |
Q15208 | P38936 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.2 | More importantly, with the direct regulation of p21 stability by phosphorylation on Ser 146, we define here the first downstream signaling mechanisms by which NDR kinases can control G1/S progression.|NDR kinases regulate p21 stability by phosphorylation of S146 on p21. | | SIGNOR-272961 |
P05107 | O60674 | 0 | phosphorylation | up-regulates activity | 0.265 | PTKs of the JAK and SRC families have a regulatory role in LFA-1 affinity triggering, with JAKs showing a positive role (3), whereas SRCs possibly have a negative role. | SIGNOR-254738 |
P07948 | P53396 | 1 | phosphorylation | up-regulates activity | 0.2 | We demonstrate the binding of PIP2 to the CoA-binding domain of ACLY and identify the six tyrosine residues of ACLY that are phosphorylated by Lyn. Three of them (Y682, Y252, Y227) can be also phosphorylated by Src and they are located in catalytic, citrate binding and ATP binding domains, respectively. PI3K and Lyn i... | SIGNOR-274105 |
Q15797 | P36894 | 0 | phosphorylation | up-regulates activity | 0.732 | Two types of bmp-induced signaling pathways are known, the smad and p38 mapk pathways. In the former case, bmpr1 phosphorylates smad-1,-5,-8, which forms a complex with smad4 that translocates into the nucleus and regulates gene expression. | SIGNOR-255263 |
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