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P51582
P19086
1
binding
up-regulates activity
0.2
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257122
O00444
O00444
2
phosphorylation
up-regulates
0.2
Autophosphorylation probably plays a role in the process of centriole duplication, because mimicking s305 phosphorylation enhances the ability of overexpressed plk4 to induce centriole amplification. Importantly, we show that s305-phosphorylated plk4 is specifically sequestered at the centrosome contrary to the nonphos...
SIGNOR-162559
P25098
P21462
1
phosphorylation
down-regulates activity
0.2
Phosphorylation of the FPR carboxyl terminus by GRK2 is the result of a high affinity interaction and proceeds in a hierarchical manner. sequential mechanism of phosphorylation beginning with residues 328 and/or 329, followed by residues 331 and/or 332, and finally residues 334 through 339. Attenuation of receptor-med...
SIGNOR-251452
Q5S007
Q5S007
2
phosphorylation
up-regulates
0.2
Three putative autophosphorylation sites (thr-2031, ser-2032, and thr-2035) have been identified within the activation segment of the lrrk2 kinase domain based on sequence homology to mixed-lineage kinases. Phosphorylation at one or more of these sites is critical for the kinase activity of lrrk2.
SIGNOR-166470
O14965
Q9UNN5
1
phosphorylation
down-regulates activity
0.2
This study reports that aurora-a (aur-a) phosphorylates fas-associated factor-1 (faf1) at ser-289 and ser-29 our findings support the negative feedback regulation of aur-a via phosphorylation of the death-promoting protein, faf1
SIGNOR-180891
P49840
O15084
1
phosphorylation
down-regulates activity
0.2
We provide evidence for a dual kinase-mediated regulation of the PITK holoenzyme whereby PITK phosphorylation at S1017 is catalyzed by calcium/calmodulin-dependent kinase II-delta (CaMKIIdelta), promoting the subsequent phosphorylation of S1013 by glycogen synthase kinase-3 (GSK3) in vitro.|the phosphorylation of PITK ...
SIGNOR-264792
P68400
Q8N163
1
phosphorylation
up-regulates activity
0.2
CK2alphawas bound to DBC1 and phosphorylated DBC1. The phosphorylation of DBC1 by CK2alphawas evidenced by co-immunoprecipitation of CK2alphaand DBC1 in a GST pull-down assay, an in vitro kinase assay, and immunofluorescence staining. |In our results, CK2alpha affected the|These results suggest that DBC1 may be involve...
SIGNOR-267666
P25103
P08754
1
binding
up-regulates activity
0.2
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257161
P01241
P24385
1
transcriptional regulation
up-regulates quantity by expression
0.2
Autocrine hGH increased the transcription and subsequent mRNA level and protein expression of c-Myc, Cyclin D1, and Bcl-2 in human mammary epithelial cells
SIGNOR-261629
Q68D86
Q5TZA2
1
binding
up-regulates activity
0.2
CCDC102B is recruited to the centrosome by C-Nap1 (also known as CEP250) and interacts with the centrosome linker components rootletin and LRRC45. CCDC102B decorates and facilitates the formation of rootletin filaments. Furthermore, CCDC102B is phosphorylated by Nek2A (an isoform encoded by NEK2) and is disassociated f...
SIGNOR-275628
P07858
P0DTC2
1
cleavage
up-regulates activity
0.2
SARS-2-S can use both CatB/L as well as TMPRSS2 for priming in these cell lines.
SIGNOR-260738
P68400
O14737
1
phosphorylation
up-regulates
0.2
Programmed cell death 5 (pdcd5), a protein involved in cell death and down-regulated in different forms of human tumors. Pdcd5 is phosphorylated in vitro by both ck2alpha subunit and by the ck2 holoenzyme at a residue, s118, which is found phosphorylated in vivo. Transfection of the non-phosphorylatable mutant (s118a) ...
SIGNOR-187106
Q86Y07
P04406
1
phosphorylation
up-regulates activity
0.2
Mechanistically, FBXW10 promotes GAPDH polyubiquitination and activation; VRK2-dependent phosphorylation of GAPDH Ser151 residue is critical for GAPDH ubiquitination and activation. 
SIGNOR-277840
Q96LB1
P08754
1
binding
up-regulates activity
0.2
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257172
P68400
Q9BXW9
1
phosphorylation
down-regulates activity
0.2
Here, we report a cluster of phosphosites on FANCD2 whose phosphorylation by CK2 inhibits both FANCD2 recruitment to ICLs and its monoubiquitination in vitro and in vivo. We have found that phosphorylated FANCD2 possesses reduced DNA binding activity, explaining the previous observations. 
SIGNOR-276730
P08047
Q9BSI4
1
transcriptional regulation
up-regulates quantity by expression
0.2
Transfection of a plasmid carrying the Sp1 transcription factor into Sp-deficient SL2 cells strongly activated TIN2 promoter-driven luciferase reporter expression.
SIGNOR-271698
Q5T0T0
P13762
1
polyubiquitination
down-regulates quantity by destabilization
0.2
Two E3 ligases, MARCH I and MARCH VIII, have been shown to polyubiquitinate lysine residue 225 in the cytoplasmic tail of I-Abeta and HLA-DRbeta. We show that lysine residue 219 in the cytoplasmic tail of DRalpha is also subject to polyubiquitination.
SIGNOR-271407
P19838
P12644
1
transcriptional regulation
down-regulates quantity by repression
0.2
 The effect of TNF-alpha on the Bmp4 promoter is mediated through NF-kB.
SIGNOR-266086
P52333
P52333
2
phosphorylation
up-regulates
0.2
The phosphorylation of wt jak3 and y980f/y981f/y785f mutant jak3 is presumably mediated through autophosphorylation at distinct jak3 sites within this model systemhosphorylation of jak3 on y785 has been reported to create a binding site for the adaptor protein sh2b-beta
SIGNOR-160660
Q07812
Q07812
2
binding
up-regulates activity
0.2
Following bid-induced conformational change, bax oligomerizes and inserts tightly within the outer mitochondrial membrane. The integration of bax in the outer mitochondrial membrane is followed by cytochrome crelease
SIGNOR-73895
Q9HCK8
P50553
1
transcriptional regulation
down-regulates quantity
0.2
Many of the most significantly up-regulated genes in Chd8+/− and Chd8−/− NPCs are involved in later stages of neuronal development, including Ascl1 [a central driver of neural reprogramming (29)], Dcx, Map2, Nefm, Neurod4, and Neurog1 (Fig. 2 E and F). Additionally, we found that Sox3 is derepressed in both Chd8+/− and...
SIGNOR-268914
P02489
P43320
1
binding
up-regulates activity
0.2
Aberrant protein interactions can lead to aggregation and insolubilization, such as occurs during cataract formation. Deamidation, a prevalent age-related modification in the lens of the eye, decreases stability of the major lens proteins, crystallins. Deamidation did not disrupt specific αA/βB2 interactions but favore...
SIGNOR-252155
Q92585
P62805
1
acetylation
down-regulates activity
0.2
We speculated that maml1, in addition to recruiting p300, might directly interact with histones to facilitate histone acetylation. We had observed acetylation of the histones h3 and h4.
SIGNOR-153041
Q16549
P30305
1
phosphorylation
down-regulates
0.2
We propose that regulation of cdc25b phosphorylation by p38 is a critical event for initiating the g2/m checkpoint after ultraviolet radiation
SIGNOR-107423
P05771
P05771
2
phosphorylation
up-regulates
0.2
The catalytic or kinase domain requires phosphorylation at three sites for full activation (24, 25): ? Phosphorylation of threonine 500 (thr-500) in the activation loop by the upstream kinase pdk-1 is a prerequisite for the maturation of the enzyme (26), which subsequently leads to autophosphorylation at threonine 641 ...
SIGNOR-150865
Q96QT6
Q04726
1
binding
up-regulates activity
0.2
We have cloned and characterized a new member of the PHD zinc finger family called Pf1 that interacts with two global transcription corepressors: mSin3A and TLE. Pf1 interacts with TLE. The Groucho/TLE proteins are members of an abundant corepressor family, and we hypothesized that Pf1 might interact with TLE family m...
SIGNOR-266993
P08238
Q8NFG4
1
binding
up-regulates quantity by stabilization
0.2
Heat shock protein-90 (Hsp90) is an essential molecular chaperone in eukaryotes involved in maintaining the stability and activity of numerous signalling proteins, also known as clients. Hsp90 ATPase activity is essential for its chaperone function and it is regulated by co-chaperones. Here we show that the tumour supp...
SIGNOR-261418
Q13131
P26358
1
phosphorylation
down-regulates activity
0.2
Together, these results indicate that AMPK phosphorylated DNMT1-Ser730, RBBP7-Ser314, and HAT1-Ser190|AMPK decreased DNMT1 activity
SIGNOR-264783
Q14493
Q9BTM1
1
translation regulation
up-regulates quantity by expression
0.2
Synthesis of mature histone mRNA requires only a single processing reaction: an endonucleolytic cleavage between a conserved stem-loop and a purine-rich downstream element to form the 3' end. The stem-loop binding protein (SLBP) is required for processing, and following processing, histone mRNA is transported to the cy...
SIGNOR-265412
Q05086
O60936
1
ubiquitination
down-regulates quantity by destabilization
0.2
Incubation of transfected HEK293T cells with the proteasome inhibitor, MG132 blocked Ube3A mediated degradation of Arc suggesting that Ube3A degrades Arc via the ubiquitin proteasome system .|The ubiquitination of Arc by Ube3A was confirmed by mass spectrometry which revealed that Ube3A catalyzed the polyubiquitination...
SIGNOR-278522
Q6U7Q0
Q9H9S0
1
transcriptional regulation
up-regulates quantity by expression
0.2
Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays revealed that Zfp322a binds to Pou5f1 and Nanog promoters and regulates their transcription.
SIGNOR-264899
O60260
O43236
1
ubiquitination
down-regulates quantity by destabilization
0.2
Thus, Parkin degrades ARTS in a proteasome dependent manner.|Together, these results suggest that Parkin specifically ubiquitinates and degrades ARTS, and that this degradation may be linked to the pro-apoptotic function of ARTS.
SIGNOR-278642
Q00839
P01375
1
post transcriptional regulation
up-regulates quantity by stabilization
0.2
In the present study, we show that hnRNP-U specifically enhances the expression of tumor necrosis factor alpha mRNA by increasing its stability, possibly through binding to the 3' untranslated region. We also show that hnRNP-U enhances the expression of several other genes as well, including GADD45A, HEXIM1, HOXA2, IER...
SIGNOR-262281
P05129
P09211
1
phosphorylation
up-regulates activity
0.2
Peptide phosphorylation analyses and both phosphorylation and enzyme kinetic studies with GSTP1 proteins mutated at candidate amino acid residues established Ser-42 and Ser-184 as putative phospho-acceptor residues for both kinases in the GSTP1 protein. Together, these findings show PKA- and PKC-dependent phosphorylati...
SIGNOR-276018
O00141
P08151
1
binding
down-regulates
0.2
SGK1 is known to inhibit another intrinsic pathway, the Hedgehog pathway, through downregulation of SMO and the GLI transcription factor family
SIGNOR-251672
O60260
Q9Y6H5
1
ubiquitination
down-regulates quantity by destabilization
0.2
Here we show that parkin interacts with and ubiquitinates the alpha-synuclein-interacting protein, synphilin-1.
SIGNOR-278550
Q13315
Q9Y6D9
1
phosphorylation
up-regulates activity
0.2
ATM mediated Mad1 Serine 214 phosphorylation regulates Mad1 dimerization and the spindle assembly checkpoint.|Together, these findings reveal an important role of ATM-mediated Mad1 Serine 214 phosphorylation in mitosis.
SIGNOR-278467
Q13131
Q16576
1
phosphorylation
up-regulates activity
0.2
AMPK increased HAT1 activity through phosphorylation of HAT1-Ser190 and RBBP7-Ser314| interaction between RBBP7 and HAT1 is required for acetyltransferase activity
SIGNOR-264784
P47900
P63096
1
binding
up-regulates activity
0.2
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257072
Q02156
P46940
1
phosphorylation
up-regulates
0.2
Using a mass spectrometry-based assay, we show that egf induces phosphorylation of iqgap1 ser(1443), a residue known to be phosphorylated by pkcthe nonphosphorylatable iqgap1 s1441a/s1443a had no effect. In contrast, the s1441e/s1443d mutation markedly enhanced the ability of iqgap1 to induce neurite outgrowth.
SIGNOR-128718
Q15831
O14744
1
phosphorylation
up-regulates activity
0.2
We found that PRMT5 is a bona fade substrate for LKB1. We identified T132, 139 and 144 residues, located in the TIM-Barrel domain of PRMT5, as target sites for LKB1 phosphorylation. The point mutation of PRMT5 T139/144 to A139/144 drastically decreased its methyltransferase activity, due probably to the loss of its int...
SIGNOR-277412
P49841
Q6U7Q0
1
phosphorylation
down-regulates quantity by destabilization
0.2
CK1delta and GSK3beta kinases sequentially phosphorylate ZNF322A at serine-396 and then serine-391. Moreover, the doubly phosphorylated ZNF322A protein creates a destruction motif for the ubiquitin ligase FBXW7alpha leading to ZNF322A protein destruction.
SIGNOR-264891
Q13315
A1Z1Q3
1
phosphorylation
down-regulates activity
0.2
We found that MacroD2 is exported from the nucleus upon DNA damage and that this depends on the ATM-induced phosphorylation of the MacroD2 C-terminal IDR.|ATM activation leads to phosphorylation of the MacroD2 C-terminal region.
SIGNOR-279792
O15344
P18031
1
ubiquitination
down-regulates quantity
0.2
Proteasome inhibitor treatment diminished the decrease of PTP1B (Figure 5F) caused by TRIM18 overexpression.|TRIM18 Interacts With PTP1B and Promotes PTP1B Ubiquitination.
SIGNOR-278703
P67775
Q12800
1
dephosphorylation
up-regulates
0.2
We previously established that phosphorylation of lsf in early g1 at ser-291 and ser-309 inhibits its transcriptional activity and that dephosphorylation later in g1 is required for its reactivation. This predominant cis conformation would also limit dephosphorylation of ser-291 and ser-309 by phosphatases such as pp2a
SIGNOR-167385
Q96PZ7
P0C0L5
1
binding
down-regulates quantity
0.2
CUB and sushi multiple domains 1 (CSMD1) is a relatively poorly studied large transmembrane protein of 390 kDa composed of 14 N-terminal CUB domains interspersed with complement control protein (CCP) domains followed by 15 consecutive CCP domains. The active domains of CSMD1 were then identified in CCP17-21, which were...
SIGNOR-265149
Q15300
P40763
1
phosphorylation
up-regulates activity
0.2
In addition, RET/PTC-mediated cellular transformation and proliferation of transformed cells require tyrosine 705 phosphorylation of STAT3 in NIH3T3 cells. We conclude that STAT3 activation by the RET/PTC tyrosine kinase is one of the critical signaling pathways for the regulation of specific genes, such as cyclin D1, ...
SIGNOR-260917
O00712
Q14393
1
transcriptional regulation
down-regulates quantity
0.2
By integrating transcriptomic profiling (RNA-seq) of Nfia- and Nfix-deficient GNPs with epigenomic profiling (ChIP-seq against NFIA, NFIB and NFIX, and DNase I hypersensitivity assays), we reveal that these transcription factors share a large set of potential transcriptional targets, suggestive of complementary roles f...
SIGNOR-268882
P52564
P46108
1
phosphorylation
up-regulates
0.2
Mapkk6 was shown to phosphorylate and specifically activate the p38/mpk2 sub-family of the mitogen-activated protein kinase superfamily.
SIGNOR-42384
Q14493
Q16695
1
translation regulation
up-regulates quantity by expression
0.2
Synthesis of mature histone mRNA requires only a single processing reaction: an endonucleolytic cleavage between a conserved stem-loop and a purine-rich downstream element to form the 3' end. The stem-loop binding protein (SLBP) is required for processing, and following processing, histone mRNA is transported to the cy...
SIGNOR-265417
Q8WZ60
P24941
1
polyubiquitination
down-regulates quantity by destabilization
0.2
We confirmed the formation of ubiquitin and CDK2 by different systems and further identified the E3 ligase KLHL6 as a mediator of the ubiquitination and degradation of CDK2. 
SIGNOR-272310
P06213
P06213
2
phosphorylation
up-regulates activity
0.2
We identified the major autophosphorylation sites in the insulin receptor and correlated their phosphorylation with the phosphotransferase activity of the receptor on synthetic peptides. We conclude that 1) autophosphorylation of the insulin receptor begins by phosphorylation of Tyr-1146 and either Tyr-1150 or Tyr-1151...
SIGNOR-106510
Q2M2I3
P48730
1
binding
up-regulates quantity
0.2
We identified members of the FAM83 family of proteins as partners of CK1 in cells. All eight members of the FAM83 family (FAM83A–H) interacted with the α and α-like isoforms of CK1; FAM83A, -B, -E, and -H also interacted with the δ and ε isoforms of CK1. The intrinsic catalytic activity of CK1 is not affected by or req...
SIGNOR-273767
Q15077
P09471
1
binding
up-regulates activity
0.2
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257076
P01137
P01137
2
binding
up-regulates activity
0.2
The active form of TGF-beta is a dimer stabilized by hydrophobic interactions and usually further strengthened by an intersubunit disulfide bridge.
SIGNOR-148605
P52333
Q96LC7
1
phosphorylation
up-regulates
0.2
These results suggest that the tyrosines at positions 597 and 667, contained within itim-like motifs, are likely targets of phosphorylation by several classes of signaling molecules, including lck, jak3, and emt. The tyrosine located at position y691 was also contributing to the phosphorylation of the wild-type siglec ...
SIGNOR-112479
O60341
P68431
1
demethylation
up-regulates activity
0.2
Here, we provide evidence that LSD1 (KIAA0601), a nuclear homolog of amine oxidases, functions as a histone demethylase and transcriptional corepressor. LSD1 specifically demethylates histone H3 lysine 4, which is linked to active transcription.
SIGNOR-264507
Q01196
P10147
1
transcriptional regulation
up-regulates quantity by expression
0.2
We show that RUNX1 can specifically bind to both RUNX sites but that only the proximal RUNX site is essential for PMA/ PHA stimulation of the MIP-1a promoter in Jurkat T-cells. We also show that the endogenous MIP-1a promoter is constitutively bound by RUNX1.
SIGNOR-251738
P30153
Q5FBB7
1
binding
up-regulates
0.2
Identification of subunits of protein phosphatase 2a (pp2a) as sgo1 binding proteins / pp2a is required for proper chromosome segregation and centromeric localization of sgo1 in hela cells
SIGNOR-145486
Q6UWZ7
P38398
1
binding
up-regulates
0.2
Full length abraxas binds the brct-repeats of brca1the rap80-abraxas complex may help recruit brca1 to dna damage sites in part through recognition of ubiquitinated proteins
SIGNOR-155144
P18848
P54577
1
transcriptional regulation
up-regulates quantity by expression
0.2
QRICH1 promotes the expression of translation-related genes. our combined ChIP-seq and RNA-seq analyses identified that QRICH1 and ATF4 were enriched at the promoters of these specific tRNA synthetases, and that ER stress positively regulated their transcription (Fig. 4I). Together, these findings suggest that QRICH1 a...
SIGNOR-269431
O43318
O75901
1
phosphorylation
up-regulates activity
0.2
As expected, increased expression of TAK1 induced by LV-TAK1 stimulated p-RASSF9, whereas p-MEK and p-ERK were reduced.|We further identified RASSF9 as a downstream target of TAK1 which phosphorylates RASSF9 at S284.
SIGNOR-279534
Q08117
O95343
1
binding
down-regulates activity
0.2
Biochemical and mutational analysis shows that the Six domain of both SIX3 and SIX6 strongly interact with the QD domain of TLE1 and AES. AES abrogates SIX3- and SIX6-induced phenotypes
SIGNOR-234586
P48729
Q8TB45
1
phosphorylation
down-regulates
0.2
Phosphorylation of all three serine residues in the deptor degron (ser286, ser287, and ser291) is necessary for - and directly mediates - the interaction with _trcp. ck1 phosphorylated the degron of deptor, as shown by western blotting with the phospho-specific antibody (fig. S3e-f). In contrast, mtor alone was unable ...
SIGNOR-176871
O94901
Q8NA31
1
binding
up-regulates activity
0.2
In this study, we found that SUN1 not only interacted with TERB1 but also interacted with MAJIN, and the interaction of SUN1 with MAJIN is stronger than TERB1. We also found that SUN1 interacted with SPDYA, an activator of CDK2. | It will be of great interest to test this hypothesis to fully understand the mechanisms o...
SIGNOR-263299
P47900
Q14344
1
binding
up-regulates activity
0.2
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257442
P46020
P46020
2
phosphorylation
up-regulates activity
0.2
Phk is activated in vitro by autophosphorylation. Ser1018 and at least three of the other six serine residues (Ser972, -985, and -1007) can be phosphorylated in vitro by Phk itself (autophosphorylation)
SIGNOR-250282
P0C6X7-PRO_0000037317
P00403
1
binding
down-regulates activity
0.2
This result suggests that the nsp10 protein could affect the activities of NADH and cytochrome oxidase II via a direct interaction while being involved in viral replication.
SIGNOR-260254
Q13627
Q13627
2
phosphorylation
up-regulates
0.2
Mirk kinase is activated by autophosphorylation on tyrosine at the y271/y273 site
SIGNOR-79760
Q05655
P11831
1
phosphorylation
down-regulates activity
0.2
Protein kinase C delta blocks immediate-early gene expression in senescent cells by inactivating serum response factor.|The phosphorylation of T160 of SRF by PKC delta in vitro and in vivo led to loss of SRF DNA binding activity.
SIGNOR-279347
Q9Y5H8
P05556
1
binding
up-regulates activity
0.2
The clustered protocadherins comprise the largest subfamily of the cadherin superfamily and are predominantly expressed in the nervous system. Pcdh-alpha proteins interact with beta1-integrin to promote cell adhesion.
SIGNOR-265668
Q14774
P49918
1
transcriptional regulation
up-regulates quantity by expression
0.2
In this study, we have identified cell cycle regulatory genes as downstream targets of the homeobox gene HLX in cultured trophoblast cells, namely RB1, MYC, EGR1, CDKN1C, ELK1, CCNB1, and JUN. RB1 and MYC mRNA expression was increased with HLX inactivation, whereas EGR1, CDKN1C, ELK1, CCNB1, and JUN mRNA expression was...
SIGNOR-261620
P31749
P78563
1
phosphorylation
down-regulates activity
0.2
AKT-dependent phosphorylation of the adenosine deaminases ADAR-1 and -2 inhibits deaminase activity. Coimmunoprecipitation studies and in vitro kinase assays revealed that AKT-1, -2, and -3 interact with both ADAR1p110 and ADAR2 and phosphorylate these RNA editases. Using site-directed mutagenesis of suspected AKT phos...
SIGNOR-276194
Q9NZJ5
Q08209
1
phosphorylation
down-regulates activity
0.2
CN becomes phosphorylated by PERK, decreasing its activity.|Finally, evidence is presented that PERK phosphorylates CN-A at low resting levels of Ca 2+.
SIGNOR-278933
P42684
Q14653
1
phosphorylation
up-regulates activity
0.2
The data in this study show that IRF3 is physically associated with c-Abl in vivo and directly binds to c-Abl in vitro. IRF3 is phosphorylated by c-Abl and c-Abl-related kinase, Arg, mainly at Y292.
SIGNOR-277441
O75385
P46937
1
phosphorylation
up-regulates quantity by stabilization
0.2
Mechanistically, hypoxia stimulates ULK1 to translocate into nucleus, where it interacts with and phosphorylates yes-associated protein (YAP) at Ser227, resulting in YAP stabilization through blockade of ubiquitin-proteasome system (UPS), which in turn facilitates PKM2 transcription, glycolysis, cell proliferation in v...
SIGNOR-277570
P43405
P43405
2
phosphorylation
up-regulates activity
0.2
These represented sites of tyrosine phosphorylation previously identified from the study of in vitro autophosphorylated Syk. Phosphorylation was observed on peptides corresponding to Tyr130, Tyr317, Tyr342, Tyr346, Tyr519, and Tyr520
SIGNOR-246621
O75582
P84243
1
phosphorylation
down-regulates activity
0.2
Phosphorylation at ser-11 (h3s10ph) by rps6ka4 and rps6ka5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or uv irradiation and result in the activation of genes, such as c-fos and c-jun.
SIGNOR-119233
Q96FX8
P19838
1
ubiquitination
down-regulates quantity
0.2
We identify KPC1 as the Ub ligase (E3) that binds to the ankyrin repeats domain of p105, ubiquitinates it, and mediates its processing both under basal conditions and following signaling
SIGNOR-255843
Q5T1M5
P60709
1
binding
up-regulates activity
0.2
However, we did detect WAFL binding to bothWIP and actin by immunoprecipitation (Fig. 4). In conclusion, we propose a model whereby WAFL associates toendocytic vesicles by its coiled-coil domain and is involved in actin-based movement of early endosomes via WIP and binding to actin.
SIGNOR-260595
Q92830
Q6NXT2
1
acetylation
down-regulates activity
0.2
The HAT module within the SAGA and ADA complexes acetylates histone H3, mainly on residues K9 and K14.
SIGNOR-269608
Q04759
Q9Y4G8
1
phosphorylation
up-regulates
0.2
After t-cell activation, the direct phosphorylation of rapgef2 at ser960 by pkc- theta regulates rap1 activation as well as lfa-1 adhesiveness to icam-1. Pkc- theta and its effector rapgef2 are critical factors in tcr signaling to rap1
SIGNOR-181186
Q5T0T0
O00220
1
ubiquitination
down-regulates quantity
0.2
The collective data indicate that endogenous MARCH-8 ubiquitinates TRAIL-R1 to attenuate its cell surface expression.
SIGNOR-278570
Q92830
P84243
1
acetylation
down-regulates activity
0.2
The HAT module within the SAGA and ADA complexes acetylates histone H3, mainly on residues K9 and K14.
SIGNOR-269603
P68400
Q14761
1
phosphorylation
up-regulates quantity by stabilization
0.2
We demonstrated for the first time that LPAP is a substrate for protein kinase CK2 that phosphorylates it at Ser153, presumably ensuring LPAP resistance to degradation.
SIGNOR-273629
Q13185
P68431
1
binding
up-regulates activity
0.2
A core characteristic of heterochromatin is its association with heterochromatin protein 1 (HP1) proteins, a highly conserved family of chromosomal proteins that bind to di- and trimethylated H3K9 via a conserved N-terminal domain called the chromodomain (CD) HP1 proteins are a highly conserved family of eukaryotic pr...
SIGNOR-264496
Q15831
Q15831
2
phosphorylation
up-regulates activity
0.2
It was shown that thr336 and thr366 are the major autophosphorylation sites of mouse lkb1 (sapkota et al., 2002). We confirmed these data on the human orthologues thr336 and thr363. Moreover, the enhanced stoichiometry of lkb1 autophosphorylation by strad enabled us to identify two novel sites: thr185 and thr402. We sh...
SIGNOR-101844
P43405
P31995
1
phosphorylation
up-regulates activity
0.2
Fyn and Blk definitely phosphorylate Y-282 in the ITAM of FcgRIIa/c, whereas the non-ITAM tyrosine residue (Y-275) becomes phosphorylated by Syk, as the phosphorylation of double point mutants shows. In addition to these tyrosine residues, Fyn, Blk, and Syk might phosphorylate the most C-terminal tyrosine residue (Y-29...
SIGNOR-262675
Q9NYL2
Q9NYL2
2
phosphorylation
up-regulates
0.2
Ionizing radiation induces mrk autophosphorylation and activation. Within the mrk kinase loop between the dfg (subdomain vii) and ape (subdomain viii) residues, there are three conserved threonine/serine residues (thr161, thr162, and ser165) that are important for activation.
SIGNOR-128581
P06493
P17661
1
phosphorylation
down-regulates quantity by destabilization
0.2
In line with this, we found that in Drp/MC mice desmin was hyperphosphorylated in Ser-31 by a hyperactivated Cdk-1.
SIGNOR-278331
P07947
O15524
1
phosphorylation
up-regulates quantity by stabilization
0.2
 Samples from human lymphomas that often overexpress SOCS1 also displayed SRC family kinase activation, constitutive phosphorylation of SOCS1 on Y80, and SOCS1 cytoplasmic localization. 
SIGNOR-277453
P33981
P10636
1
phosphorylation
up-regulates activity
0.2
Fig. 6C-1 shows that the GST-TTK fusion protein phosphorylated both tau and tubulin, but the phosphorylating activity on tau was much higher than that on tubulin.
SIGNOR-279132
P24723
P29474
1
phosphorylation
down-regulates activity
0.2
The phosphorylation of both S617 and S635 have also been shown to promote increased eNOS-derived NO release (Michell et al., 2002). The phosphorylaiton of S617 can be induced by PKA or Akt activity, and may serve to sensitize eNOS to calmodulin binding and modulate the phosphorylation of other eNOS sites
SIGNOR-251634
Q5T6F0
P43357
1
binding
down-regulates quantity by destabilization
0.2
 The CRL4‐DCAF12 E3 ubiquitin ligase degrades MAGE‐A3/6
SIGNOR-272254
Q96SW2
Q9UJQ4
1
binding
down-regulates quantity by destabilization
0.2
Thalidomide-induced teratogenicity is dependent on its binding to cereblon (CRBN), the substrate receptor of the Cul4A-DDB1-CRBN-RBX1 E3 ubiquitin ligase complex. Thalidomide binding to CRBN elicits subsequent ubiquitination and proteasomal degradation of CRBN neosubstrates including SALL4, a transcription factor of wh...
SIGNOR-272208
Q96EP0
P29466
2
polyubiquitination
up-regulates activity
0.2
HOIP forms a constitutive interaction with caspase-1 and mediates the linear ubiquitination of the CARD pro-domain. Upon engagement of apoptosis, caspase-1 and caspase-8 cleave HOIP at Asp-348 and Asp-387, limiting the ability of LUBAC to ubiquitinate substrates.
SIGNOR-272191
Q6NT76
P01579
1
transcriptional regulation
down-regulates quantity by repression
0.2
Additionally, by luciferase reporter assay, HMBOX1 displayed suppressive effect on the transcription activity of IFN-γ promoter. 
SIGNOR-261625
Q86UY5
P48730
1
binding
up-regulates quantity
0.2
We identified members of the FAM83 family of proteins as partners of CK1 in cells. All eight members of the FAM83 family (FAM83A–H) interacted with the α and α-like isoforms of CK1; FAM83A, -B, -E, and -H also interacted with the δ and ε isoforms of CK1. The intrinsic catalytic activity of CK1 is not affected by or req...
SIGNOR-273765
Q9UNH5
Q96R06
1
dephosphorylation
down-regulates activity
0.2
We also demonstrate that Cdc14A dephosphorylates Astrin, and therefore the overexpression of Cdc14A sequesters Astrin in the centrosome and results in aberrant chromosome alignment.
SIGNOR-277066
P31749
Q96QF0
1
phosphorylation
up-regulates activity
0.2
Rabin8 is phosphorylated and activated by Akt in cells grown on stiff ECM.
SIGNOR-277802