IdA stringlengths 6 21 | IdB stringlengths 6 21 | labels float64 0 2 | mechanism stringclasses 40
values | effect stringclasses 10
values | score float64 0.1 0.99 ⌀ | sentence stringlengths 10 1.63k ⌀ | signor_id stringlengths 12 14 |
|---|---|---|---|---|---|---|---|
P24385 | Q96PU4 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.294 | We found that NIRF directly ubiquitinated cyclins D1 and E1, as evidenced by the appearance of the tail (Fig. 4B). In summary, the above findings suggest that NIRF tightly cooperates with the core cell cycle machinery and induces G1 arrest, which is accompanied by ubiquitination of cyclins D1 and E1. | SIGNOR-271885 |
Q9ULW0 | P24941 | 0 | phosphorylation | down-regulates activity | 0.294 | In this study, we characterize the phosphorylation of threonine 72 (Thr(72)) in human TPX2, a residue highly conserved across species. We find that Cdk1/2 phosphorylate TPX2 in vitro and in vivo. |Endogenous TPX2 phosphorylated at Thr(72) does not associate with the mitotic spindle. Furthermore, ectopic GFP-TPX2 T72A p... | SIGNOR-265099 |
O14733 | O43379 | 0 | relocalization | up-regulates activity | 0.294 | In the WT brain, the WDR62 scaffold organizes a protein complex including MEKK3, MKK4/7, and JNK1 to control NPC development during corticogenesis | SIGNOR-271716 |
Q9Y6K1 | Q03112 | 0 | binding | up-regulates activity | 0.293 | The oncoprotein EVI1 and the DNA methyltransferase Dnmt3 co-operate in binding and de novo methylation of target DNA|Here we show that EVI1 physically interacts with DNA methyltransferases 3a and 3b (Dnmt3a/b), which are the only de novo DNA methyltransferases identified to date in mouse and man, and that it forms an e... | SIGNOR-273432 |
O43318 | P22607 | 0 | phosphorylation | up-regulates activity | 0.293 | Indeed, we found that TAK1 was tyrosine phosphorylated in HEK293 cells transiently expressing constitutively active FGFR3 (K650E), but not the kinase-dead receptor (K508M), indicating that activated FGFR3 can either directly or indirectly tyrosine phosphorylate TAK1 (XREF_FIG). | SIGNOR-279176 |
P63096 | P28335 | 0 | binding | up-regulates activity | 0.293 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256734 |
P04406 | Q13131 | 0 | phosphorylation | up-regulates activity | 0.293 | Under glucose starvation, but not amino acid starvation, cytoplasmic GAPDH is phosphorylated on Ser122 by activated AMPK. This causes GAPDH to redistribute into the nucleus. Inside the nucleus, GAPDH interacts directly with Sirt1, displacing Sirt1's repressor and causing Sirt1 to become activated. | SIGNOR-259857 |
O14983 | P16157 | 0 | binding | down-regulates activity | 0.293 | We recently reported that small ankyrin 1 (sAnk1) interacts with the sarco(endo)plasmic reticulum Ca2+-ATPase in skeletal muscle (SERCA1) to inhibit its activity. | SIGNOR-265927 |
P54762 | Q969H4 | 0 | binding | up-regulates activity | 0.293 | The phosphorylated CNK1 interacts with ephrinB1. The binding of ephrinB1 to CNK1 connects RhoA and p115RhoGEF with ephrinB1-associated MKK4, promoting JNK activation and cell migration. | SIGNOR-275921 |
P99999 | P31751 | 0 | phosphorylation | down-regulates activity | 0.293 | Finally, we propose that pro-survival kinase Akt (protein kinase B) is a likely mediator of the S47 phosphorylation of Cytc in the brain. | SIGNOR-277236 |
O60716 | P27361 | 0 | phosphorylation | down-regulates activity | 0.293 | Upon TGFβ treatment, activated extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylates T900 of p120-catenin to promote its interaction with Smurf1 and subsequent monoubiquitination. TGFβ promotes monoubiquitination of p120-catenin through Smurf1 to induce junction dissociation. | SIGNOR-277506 |
P22415 | P78527 | 0 | phosphorylation | up-regulates | 0.293 | Feeding induces the recruitment of dna-pk to usf-1 and its phosphorylation, which then allows recruitment of p/caf, resulting in usf-1 acetylation and fas promoter activation. | SIGNOR-184849 |
P04275 | P29122 | 0 | cleavage | up-regulates activity | 0.293 | Like PACE,PACE4 was able to process pro-vWF to its mature form, and efficient cleavage required both the P4 arginine and the P2 lysine | SIGNOR-260367 |
P00533 | Q32MZ4 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.293 | GC-binding factor 2 (GCF2) is a transcriptional repressor that decreases activity of the epidermal growth factor receptor (EGFR) and other genes. |Deletion mutants of GCF2 revealed that amino acids 429–528 are required for both DNA binding and repression of the EGFR promoter. | SIGNOR-266057 |
P30622 | P68400 | 0 | phosphorylation | up-regulates | 0.293 | Herein, we have identified polo-like kinase 1 (plk1) and casein kinase 2 (ck2) as two kinases of clip-170 and mapped s195 and s1318 as their respective phosphorylation sites.Plk1- and ck2-associated phosphorylations of clip-170 are involved in the timely formation of kinetochore-microtubule attachments in mitosis | SIGNOR-167168 |
Q96MT3 | Q8N4C8 | 0 | phosphorylation | up-regulates activity | 0.293 | We show that Mink1 phosphorylates Prickle on a conserved threonine residue and regulates its Rab5-dependent endosomal trafficking, a process required for the localized plasma membrane accumulation and function of Prickle. | SIGNOR-263095 |
O00213 | P49841 | 0 | phosphorylation | up-regulates quantity | 0.293 | In this regards, GSK3beta may promote amyloidogenic processing of APP by regulating the cellular level of monomeric FE65 for the formation of LRP1, FE65, and APP complex.|In this study, we showed that FE65 is phosphorylated at T579 by GSK3beta. | SIGNOR-278359 |
Q9HBW0 | Q8WWY8 | 0 | binding | up-regulates | 0.293 | When overexpressed in jurkat t cells, the edg4 protein mediated lpa-induced activation of a serum response element reporter gene with lpa concentration dependence (ec50 of 10 nm) and specificity. | SIGNOR-56093 |
Q5JVS0 | P05129 | 0 | phosphorylation | down-regulates activity | 0.293 | We found a strong phosphorylation of Ki-1/57 by PKCalphabeta, PKCdelta, PKClambda/zeta, and especially by PKCsigma, however not by PKCmi. These data show that Ki-1/57 can serve in principal as a substrate for a wide variety of different PKC isoforms but also that its phosphorylation is strongest with PKCsigma. | This s... | SIGNOR-249255 |
Q14524 | P06241 | 0 | phosphorylation | down-regulates | 0.293 | This study addresses the effects of the src family tyrosine kinase fyn on na(v)1.5 cardiac sodium channels. Sodium currents were acquired by whole cell recording on hek-293 cells transiently expressing na(v)1.5. Acute treatment of cells with insulin caused a depolarizing shift in steady-state inactivation, an effect el... | SIGNOR-135600 |
Q8IXJ6 | Q13627 | 0 | phosphorylation | up-regulates activity | 0.293 | This Minibrain/Dyrk1a kinase phosphorylates and activates the Silent information regulator 2/Sirtuin1 deacetylase, which in turn deacetylates and activates the FOXO transcription factor. | SIGNOR-279990 |
Q13887 | P04150 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.292 | We show that in addition, DEX-bound GR directly promotes the expression of adipogenic TFs, including C/EBPβ, Klf5, Klf9, and C/EBPα | SIGNOR-256118 |
Q13131 | P31749 | 0 | phosphorylation | down-regulates activity | 0.292 | It is proposed that the effect of insulin to antagonize AMP-activated protein kinase activation involves a hierarchical mechanism whereby Ser 485/Ser 491 phosphorylation by protein kinase B reduces subsequent phosphorylation of Thr 172 by LKB1 and the resulting activation of AMP-activated protein kinase. | SIGNOR-252739 |
P35226 | P31751 | 0 | phosphorylation | up-regulates activity | 0.292 | the polycomb group silencing protein Bmi1 can be phosphorylated by AKT, which enhances its oncogenic potential in PCa. Overexpression of Bmi1 can act in combination with PTEN haploinsufficiency to induce invasive carcinogenic formation in the prostate | SIGNOR-249582 |
Q5JVS0 | Q05513 | 0 | phosphorylation | down-regulates activity | 0.292 | We found a strong phosphorylation of Ki-1/57 by PKCalphabeta, PKCdelta, PKClambda/zeta, and especially by PKCsigma, however not by PKCmi. These data show that Ki-1/57 can serve in principal as a substrate for a wide variety of different PKC isoforms but also that its phosphorylation is strongest with PKCsigma. | This s... | SIGNOR-249257 |
Q14332 | Q9ULT6 | 0 | ubiquitination | down-regulates | 0.292 | Znrf3 is associated with the wnt receptor complex, and inhibits wntby promoting the turnover of frizzled and lrp6. | SIGNOR-197417 |
P50549 | P17612 | 0 | phosphorylation | up-regulates activity | 0.292 | The camp-dependent protein kinase a (pka) phosphorylates er81 on ser(191)/ser(216)ser(191) and ser(216), were identified, whose mutation to alanine reduces er81 activity upon erk-mapk stimulation. | SIGNOR-92447 |
P51795 | Q96PU5 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.292 | The presence of albumin triggers the formation of an endocytic complex that includes ClC-5. (iii) Nedd4-2 is recruited to this complex and ubiquitinates ClC-5. This ubiquitination by Nedd4-2 shunts ClC-5 into the albumin uptake/degradative pathway. | SIGNOR-272665 |
Q13164 | P15056 | 0 | phosphorylation | up-regulates quantity | 0.292 | Our data indicate that oncogenic BRAF increases ERK5 protein level, phosphorylation at several residues and kinase activity.|Overexpression of oncogenic BRAF induced ERK5 phosphorylation at Thr218 and Tyr220, although to a lower level than that induced by MEK5DD. | SIGNOR-278353 |
Q9P0J1 | P11362 | 0 | phosphorylation | down-regulates activity | 0.292 | Here we report that phosphorylation at another tyrosine residue, Tyr-94, inhibits PDP1 by reducing the binding ability of PDP1 to lipoic acid, which is covalently attached to the L2 domain of dihydrolipoyl acetyltransferase (E2) to recruit PDP1 to PDC. We found that multiple oncogenic tyrosine kinases directly phosphor... | SIGNOR-276640 |
Q9UMX1 | Q9UKB1 | 0 | binding | up-regulates | 0.292 | We found that in vitro-translated 35s- labeled slimb indeed specifically bound to su(fu) in the gst pull-down assay. In our functional gli reporter assay, slimb alone did not alter gli-induced reporter expression;however, when cotransfected with hsu(fu), slimb significantly potentiated the inhibitory effect of su(fu) o... | SIGNOR-72240 |
P63096 | P29275 | 0 | binding | up-regulates activity | 0.292 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-257039 |
P38405 | P33032 | 0 | binding | up-regulates activity | 0.292 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256941 |
P35498 | Q96PU5 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.292 | The control of Nav density at the cell membrane is crucial to ensuring normal neuronal excitability. Navs are subject to posttranslational modifications that may influence their cell membrane availability. Ubiquitylation is a key process that orchestrates the internalization and subsequent degradation or recycling of ... | SIGNOR-253457 |
P04629 | P12931 | 0 | phosphorylation | up-regulates activity | 0.292 | Direct phosphorylation of TrkA by Src family kinases has been demonstrated in vitro, and our studies suggest that Src may lead to selective activation of TrkA. | SIGNOR-279291 |
P63092 | P24530 | 0 | binding | up-regulates activity | 0.292 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256781 |
P60709 | P12277 | 0 | relocalization | up-regulates quantity | 0.292 | In summary, data presented here strongly suggest that locally generated ATP is an important regulator for actin-based cytoskeletal dynamics involved in cell extension and motility and that CK-B is a controlling enzyme in the compartmentalization of ATP availability. CK-B co-localizes with cortical actin and facilitates... | SIGNOR-265791 |
P31277 | P09429 | 0 | binding | up-regulates activity | 0.292 | We show that HMG1 interacts with proteins encoded by the HOX gene family by establishing protein-protein contacts between the HMG box domains and the HOX homeodomain. HMG1 enhances, in a dose-dependent fashion, the sequence-specific DNA binding activity in vitro, and the transcriptional activation in a co-transfection ... | SIGNOR-240559 |
P25815 | Q9UNE7 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.292 | S100 protein itself is ubiquitinated by CHIP in a Ca2+-dependent manner.Ubiquitylated S100 proteins are shown as (Ub)n-S100A2 and (Ub)n-S100P. The association of the S100 proteins with CHIP provides a Ca2+-dependent regulatory mechanism for the ubiquitination and degradation of intracellular proteins by the CHIP-protea... | SIGNOR-272919 |
P28358 | P09429 | 0 | binding | up-regulates activity | 0.292 | We show that HMG1 interacts with proteins encoded by the HOX gene family by establishing protein-protein contacts between the HMG box domains and the HOX homeodomain. HMG1 enhances, in a dose-dependent fashion, the sequence-specific DNA binding activity in vitro, and the transcriptional activation in a co-transfection ... | SIGNOR-240556 |
P36888 | P06241 | 0 | phosphorylation | up-regulates activity | 0.292 | FYN phosphorylates FLT3 on tyrosine residues (A\u2013B) COS1 cells were transfected with plasmids expressing FLT3 wild-type and FYN wild-type or mutants.|It was not completely unexpected that FYN associates wild-type FLT3 in the absence of ligand stimulation in COS-1 cells, as overexpression of wild-type FLT3 results i... | SIGNOR-279715 |
Q01094 | P27361 | 0 | phosphorylation | up-regulates | 0.292 | Erk also undergoes rapid translocation into the nucleus, where it phosphorylates and activates a variety of transcription factor targets, including sp1, e2f, elk-1, and ap1 | SIGNOR-121991 |
P63000 | Q38SD2 | 0 | phosphorylation | up-regulates activity | 0.292 | In vitro kinase assays confirmed that recombinant Lrrk1 phosphorylated RAC1-GST protein, and immunoprecipitation showed that the interaction of Lrrk1 with RAC1 occurred within 10 min after RANKL treatment.|Lrrk1 phosphorylates and activates RAC1 and Cdc42 small GTPase proteins in osteoclasts. | SIGNOR-279626 |
P24941 | P35813 | 0 | dephosphorylation | down-regulates activity | 0.292 | Moreover, purified recombinant PP2C alpha and PP2C beta 2 proteins efficiently dephosphorylated monomeric Cdk2/Cdk6 in vitro. | SIGNOR-277107 |
P29317 | Q15418 | 0 | phosphorylation | up-regulates activity | 0.292 | These comprehensive analyses indicate that high matrix stiffness activates ERK/RSK1-mediated EPHA2 non-canonical signalling to induce LYN activation, TWIST1 nuclear localization, and cell invasion (Fig. 6M).|We next knocked down the most abundant RSK family members and found that loss of RSK1, but not RSK2 or RSK3, dra... | SIGNOR-280113 |
P49715 | P35712 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.292 | We found that SOX6 regulates adipogenesis in vertebrate species by activating adipogenic regulators including PPARγ, C/EBPα and MEST | SIGNOR-255824 |
O75364 | Q9H334 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.292 | We identified FoxP1 as a novel marker for midbrain dopamine neurons. Enforced expression of FoxP1 in embryonic stem cells actuates the expression of Pitx3, a homeobox protein that is exclusively expressed in midbrain dopaminergic neurons and is required for their differentiation and survival during development and from... | SIGNOR-269049 |
P15172 | P26651 | 0 | post transcriptional regulation | down-regulates quantity by destabilization | 0.292 | The TTP binding site in the 3′ UTR of MyoD would permit TTP-mediated mRNA decay | SIGNOR-253597 |
O00141 | P51608 | 0 | transcriptional regulation | down-regulates quantity by repression | 0.292 | These results are compatible with the hypothesis that MeCP2 associates with the Sgk and Fkbp5 promoters and has a repressive effect that is over-ridden by elevated glucocorticoids in response to stress. | SIGNOR-264543 |
Q8IXJ6 | P12931 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.291 | In this study, we investigated the potential regulation of SIRT2 function by c-Src. We found that the protein levels of SIRT2 were decreased by c-Src, and subsequently rescued by the addition of a Src specific inhibitor, SU6656, or by siRNA-mediated knockdown of c-Src. The c-Src interacts with and phosphorylates SIRT2 ... | SIGNOR-263104 |
Q8WYL5 | Q9BZL6 | 0 | phosphorylation | down-regulates | 0.291 | Phosphorylation of ser 402 impedes phosphatase activity of slingshot 1. | SIGNOR-173441 |
Q05397 | Q13164 | 0 | phosphorylation | up-regulates activity | 0.291 | Remarkably, the reduction in ERK5 expression correlated with decreased p-FAK(Tyr397) staining, consistent with the requirement of ERK5 for mediating FAK activation in vivo (Fig. 6C).|We confirmed that JWG-045, a novel pharmacological inhibitor of ERK5 exhibiting significantly reduced affinity for BRD4 compared with XMD... | SIGNOR-279304 |
Q9NZH5 | P06493 | 0 | phosphorylation | up-regulates activity | 0.291 | HPTTG is phosphorylated by Cdc2 at Ser165. we show that hPTTG is phosphorylated during mitosis. The direct phosphorylation of hPTTG by Cdc2 is interesting in itself since the substrates of this master mitotic kinase are supposed to play important roles in the initiation and progression of mitosis. | SIGNOR-262700 |
P04150 | P24941 | 0 | phosphorylation | up-regulates activity | 0.291 | Cyclin-dependent kinase (CDK) and mitogen-activated protein kinase (MAPK) phosphorylate the rat glucocorticoid receptor in vitro at distinct sites that together correspond to the major phosphorylated receptor residues observed in vivo; MAPK phosphorylates receptor residues threonine 171 and serine 246, whereas multiple... | SIGNOR-249427 |
P36894 | O95476 | 0 | binding | up-regulates | 0.291 | We show that dullard promotes the ubiquitin-mediated proteosomal degradation of bmp receptors (bmprs). Dullard preferentially complexes with the bmp type ii receptor (bmprii) and partially colocalizes with the caveolin-1-positive compartment, suggesting that dullard promotes bmpr degradation via the lipid raft-caveolar... | SIGNOR-150998 |
P63092 | P25100 | 0 | binding | up-regulates activity | 0.291 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256808 |
P34897 | P01106 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.291 | Myc regulates the de novo purine and pyrimidine synthetic genes in multiple biological systems. Intriguingly, MYC was found to directly activate the expression of SHMT1, and SHMT2, which are enzymes involved in single carbon metabolism and are essential for dNTP synthesis | SIGNOR-267380 |
Q08999 | O43524 | 0 | transcriptional regulation | up-regulates quantity | 0.291 | Here we show that the Forkheads AFX (FOXO4) and FKHR-L1 (FOXO3a) also directly control transcription of the retinoblastoma-like p130 protein and cause upregulation of p130 protein expression. | SIGNOR-238606 |
Q969H0 | O00141 | 0 | phosphorylation | up-regulates | 0.291 | Here, we report that the serum- and glucocorticoid-inducible protein kinase sgk1 remarkably reduced the protein stability of the active form of notch1 through fbw7activated sgk1 phosphorylated fbw7 at serine 227 | SIGNOR-170404 |
P43405 | P46934 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.291 | The latent membrane protein (LMP) 2A of Epstein-Barr virus (EBV) is implicated in the maintenance of viral latency and appears to function in part by inhibiting B-cell receptor (BCR) signaling. LMP2A enhances Lyn and Syk ubiquitination in vivo in a fashion that depends on the activity of Nedd4 family members and correl... | SIGNOR-272581 |
Q9UGP8 | P68400 | 0 | phosphorylation | up-regulates activity | 0.291 | Sec63 was identified as a novel substrate and binding partner of protein kinase CK2. We identified serine 574, serine 576 and serine 748 as CK2 phosphorylation sites. Phosphorylation of Sec63 by CK2 enhanced its binding to Sec62. | SIGNOR-265267 |
P62380 | O96017 | 0 | phosphorylation | down-regulates activity | 0.291 | In vitro, TRF2 was phosphorylated by recombinant Chk2 ( Figure\u00a04 C) on residues located in the first 350 aa ( Figure\u00a0S11 ).|To evaluate whether Chk2 activity affected the binding of TRF2 for telomeric TTAGGG repeats in duplex DNA, electrophoretic mobility shift assays (EMSA) were performed in the presence of ... | SIGNOR-280227 |
P56962 | Q9UHD2 | 0 | phosphorylation | up-regulates activity | 0.291 | Stx17 is phosphorylated by TBK1 whereby phospho-Stx17 controls the formation of the ATG13+FIP200+ mammalian pre-autophagosomal structure (mPAS) in response to induction of autophagy. TBK1 phosphorylates Stx17 at S202. | SIGNOR-273812 |
P13726 | Q16539 | 0 | phosphorylation | down-regulates | 0.291 | We previously showed that the phosphorylation of ser253 within the cytoplasmic domain of human tissue factor (tf) initiates the incorporation and release of this protein into cell-derived microparticles. Furthermore, subsequent phosphorylation of ser258 terminates this process. Our current study has identified p38_ as ... | SIGNOR-199868 |
P41594 | Q04759 | 0 | phosphorylation | up-regulates activity | 0.291 | Thus, we showed that it is phosphorylation of Ser-839, not Thr-840, that is absolutely required for the unique Ca2+ oscillations produced by mGluR5 activation. The Thr-840 residue is important only in that it is permissive for the PKC-dependent phosphorylation of Ser-839. | SIGNOR-249290 |
Q14524 | Q13555 | 0 | phosphorylation | up-regulates activity | 0.291 | Among the sites identified, only six were previously suggested to be the targets for specific kinases using in silico and/or in vitro analyses: S36 and S525 were attributed to the regulation by PKA; S484 and S664 were assigned to the serum- and glucocorticoid-inducible kinase 3 (SGK3); and S516 and S571 were ascribed t... | SIGNOR-275779 |
P12931 | P31943 | 0 | post transcriptional regulation | up-regulates quantity by expression | 0.291 | HnRNP H is a component of a splicing enhancer complex that activates a c-src alternative exon in neuronal cells. | SIGNOR-261273 |
P63092 | P35348 | 0 | binding | up-regulates activity | 0.291 | Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali... | SIGNOR-256812 |
Q01543 | Q09472 | 0 | binding | up-regulates | 0.291 | P300 promotes the interaction of fli1 with hdac1 and increases the dna binding ability of fli1 through deacetylation of lysine 380 | SIGNOR-202682 |
Q13188 | Q9H2K8 | 0 | phosphorylation | up-regulates | 0.291 | In addition, the thousand-and-one (tao) amino acids kinase or taok1 3 has been shown to directly phosphorylate and activate hpo or mst1/2 | SIGNOR-201333 |
P04150 | P62136 | 0 | dephosphorylation | up-regulates activity | 0.291 | The current study assessed whether PP1\u03b1 can stimulate GR function and tested two different hypotheses: First, that PP1\u03b1 regulates GR activity through suppression of MDM2 activity by dephosphorylating it at Ser166, thereby reducing the MDM2-mediated ubiquitination of GR and the subsequent proteasomal degradati... | SIGNOR-277161 |
P03372 | O00170 | 0 | transcriptional regulation | down-regulates activity | 0.291 | The immunophilin-like protein XAP2 is a negative regulator of estrogen signaling through interaction with estrogen receptor α. | SIGNOR-253644 |
Q9UPN4 | P49137 | 0 | phosphorylation | down-regulates quantity | 0.291 | We identify CEP131 as a major Centriolar satellites-associated substrate of p38-dependent, MK2-mediated phosphorylation on two defined residues and show that these modifications promote binding to 14-3-3 proteins, in turn leading to cytoplasmic sequestration of CEP131 and associated Centriolar satellites factors.|We th... | SIGNOR-278181 |
Q8N1W1 | P50148 | 0 | binding | up-regulates activity | 0.291 | Taken together, the results suggest that active G13 and Gq form a complex with Rgnef and that G13 and Gq are upstream activators of Rgnef. | SIGNOR-278065 |
P23771 | Q15797 | 0 | transcriptional regulation | up-regulates quantity | 0.291 | Chromatin immunoprecipitation (ChIP) revealed a subset of the BIG (BMP4 induced genes) signature, including Satb2, Smad6, Hand1, Gadd45γ and Gata3, that was bound by Smad1/5 in the developing mandible, revealing direct Smad-mediated regulation | SIGNOR-268938 |
P40763 | Q13233 | 0 | phosphorylation | up-regulates activity | 0.291 | Phosphorylation of s727 induces pin1 binding which increases transcription. Pin1 binding increases stat3 interaction with p300 and dna. | SIGNOR-236346 |
Q13131 | Q8IWQ3 | 0 | phosphorylation | up-regulates | 0.291 | Ampka1 activators increased phosphorylation level and cytoplasmic localization (reduced nuclear/cytoplasmic ratio). Ampka1 activators reduced rna synthesis in the nucleoli. | SIGNOR-176594 |
P56178 | Q16539 | 0 | phosphorylation | up-regulates activity | 0.291 | We show that Dlx5 is a novel substrate for p38 MAPK in vitro and in vivo and that Ser-34 and Ser-217 are the sites phosphorylated by p38 | SIGNOR-255792 |
Q92974 | Q16512 | 0 | phosphorylation | down-regulates | 0.291 | Here we identify a region in the carboxyl terminus of gef-h1 that is important for suppression of its guanine nucleotide exchange activity by microtubules. This portion of the protein includes a coiled-coil motif, a proline-rich motif that may interact with src homology 3 domain-containing proteins, and a potential bin... | SIGNOR-122191 |
Q15375 | P31271 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.291 | Analysis of normalized luciferase expression confirmed that wt HOXA13 regulates gene expression through the EphA7 cis-regulatory DNA element | SIGNOR-261631 |
P49840 | Q13554 | 0 | phosphorylation | down-regulates | 0.291 | Inhibitory phosphorylation of gsk-3 by camkii couples depolarization to neuronal survival. | SIGNOR-167962 |
Q6IE81 | Q8IUQ4 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.291 | Siah-1 decreases Jade-1 abundance and enhances Jade-1 ubiquitination | SIGNOR-272915 |
Q70YC5 | P04637 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.29 | Here, analysis of p53-regulated genes activated in the setting of telomere dysfunction identified Zfp365 (ZNF365 in humans) as a direct p53 target that promotes genome stability| Our study identified ZNF365 as a necessary target whose activation by p53 in the presence of critically short telomeres contributes to genomi... | SIGNOR-272476 |
Q5JVS0 | P17252 | 0 | phosphorylation | down-regulates activity | 0.29 | We found a strong phosphorylation of Ki-1/57 by PKCalphabeta, PKCdelta, PKClambda/zeta, and especially by PKCsigma, however not by PKCmi. These data show that Ki-1/57 can serve in principal as a substrate for a wide variety of different PKC isoforms but also that its phosphorylation is strongest with PKCsigma. | This s... | SIGNOR-249246 |
P43699 | P84022 | 0 | binding | down-regulates activity | 0.29 | TGF-beta represses transcription of pulmonary surfactant protein-B gene in lung epithelial cells. Repression is mediated by SMAD3 through interactions with NKX2.1 and FOXA1, two key transcription factors that are positive regulators of SpB transcription. In this study, we found that SMAD3 interacts through its MAD doma... | SIGNOR-254169 |
P46531 | Q6UY11 | 0 | binding | down-regulates activity | 0.29 | Moreover, the interaction of DLK1 with NOTCH1 caused an inhibition of basal NOTCH signaling in preadipocytes and mesenchymal multipotent cells. In this work, we demonstrate, for the first time, that DLK2 interacts with itself, with DLK1, and with the same NOTCH1 receptor region as DLK1 does. We demonstrate also that th... | SIGNOR-219377 |
O75116 | P55040 | 0 | binding | down-regulates activity | 0.29 | Two functions for Gem have been demonstrated, including inhibition of voltage-gated calcium channel activity and inhibition of Rho kinase-mediated cytoskeletal reorganization, such as stress fiber formation and neurite retraction. These functions for Gem have been ascribed to its interaction with the calcium channel Β ... | SIGNOR-261711 |
O95630 | O00238 | 0 | phosphorylation | up-regulates activity | 0.29 | BMP type I receptor activation stimulates AMSH phosphorylation | The exact position of phosphoserine residues in four phosphopeptides was identified by Edman degradation analysis; spot a for Ser243, Ser245 and Ser247, spot b for Ser2, and spots c and d for Ser48. To confirm the position of the phosphoserine residues, t... | SIGNOR-250600 |
Q96AQ6 | Q9UHD2 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.29 | Phosphorylation of HPIP on serine 147 by TBK1 promotes E2-mediated GREB1 expression. Accordingly, we identified the microtubule-associated HPIP, a positive regulator of oncogenic AKT signaling, as a novel MDM2 substrate. MDM2-dependent HPIP degradation occurs in breast cancer cells on its phosphorylation by the estroge... | SIGNOR-273643 |
Q96AQ6 | Q00987 | 0 | polyubiquitination | down-regulates quantity by destabilization | 0.29 | . Accordingly, we identified the microtubule-associated HPIP, a positive regulator of oncogenic AKT signaling, as a novel MDM2 substrate. MDM2-dependent HPIP degradation occurs in breast cancer cells on its phosphorylation by the estrogen-activated kinase TBK1. | SIGNOR-272850 |
Q86UR1 | P17252 | 0 | phosphorylation | down-regulates | 0.29 | Phosphorylation of nadph oxidase activator 1 (noxa1) on serine 282 by map kinases and on serine 172 by protein kinase c and protein kinase a prevents nox1 hyperactivation. | SIGNOR-163667 |
P14618 | P36956 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.29 | Well-described targets of srebp-1 and the carbohydrate response element binding protein (chrebp), which include the following: fatty acid synthase (fas), acetyl coa carboxylase (acc1), and liver pyruvate kinase (l-pk) | SIGNOR-166381 |
Q5JVS0 | Q05655 | 0 | phosphorylation | down-regulates activity | 0.29 | We found a strong phosphorylation of Ki-1/57 by PKCalphabeta, PKCdelta, PKClambda/zeta, and especially by PKCsigma, however not by PKCmi. These data show that Ki-1/57 can serve in principal as a substrate for a wide variety of different PKC isoforms but also that its phosphorylation is strongest with PKCsigma. | This s... | SIGNOR-249254 |
Q13422 | P68400 | 0 | phosphorylation | down-regulates | 0.29 | We identified four novelikarosphosphorylation sites that are phosphorylated by ck2 kinase. / ck2-mediated phosphorylation inhibits ikaros' localization to pc-hc / hyperphosphorylation of ikaros promotes its degradation by the ubiquitin/proteasome pathway / these results suggest that ck2 kinase directly phosphorylates a... | SIGNOR-174832 |
P51168 | P28482 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.29 | Using a number of different approaches it was demonstrated that the protein kinase acting on betaThr-613 and gammaThr-623 is the extracellular regulated kinase (ERK). It is suggested that an ERK-mediated phosphorylation of betaThr-613 and gammaThr-623 down-regulates the channel by facilitating its interaction with Nedd... | SIGNOR-249446 |
Q16875 | O14920 | 0 | phosphorylation | down-regulates activity | 0.29 | IKKβ promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3.We demonstrate that IKKβ directly interacts with and phosphorylates 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoform 3 (PFKFB3), a major driver of aerobic glycolysis, at Ser269 upon glutamine deprivation ... | SIGNOR-277278 |
P51149 | Q15904 | 0 | binding | up-regulates activity | 0.29 | We found that Ac45 colocalized with Rab7 in resorbing osteoclasts cultured on bone slices (Fig. 6A). In addition, a co-immunoprecipitation assay revealed that Ac45 directly interacted with Rab7| Therefore, Ac45’s role in extracellular acidification, lysosomal trafficking, and cathepsin K exocytosis may be through the R... | SIGNOR-261484 |
O00401 | P17706 | 0 | dephosphorylation | down-regulates | 0.29 | Similarly, the t cell phosphatase has a 30-fold lower kcat/km toward autoinhibited p-n-wasp than toward the isolated p-gbd, and again this effect is largely reversed by that cdc42 | SIGNOR-141652 |
P53350 | P00519 | 0 | phosphorylation | up-regulates activity | 0.29 | C-ABL can directly phosphorylate PLK1 and activate PLK1. | The above results indicate that c-ABL–mediated PLK1 Y425 phosphorylation regulates PLK1 ubiquitination and stability. | SIGNOR-260935 |
P04114 | Q9UKV5 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.29 | In physiological condition, the unnecessary apoB is usually ubiquitinated by E3 ligase AMFR, and subsequently degraded by ubiquitination proteasomes. | SIGNOR-278685 |
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