Datasets:
Tasks:
Image Segmentation
Sub-tasks:
instance-segmentation
Languages:
English
Size:
1K - 10K
License:
minor changes
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by Juicstus - opened
README.md
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task_ids:
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- instance-segmentation
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size_categories:
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dataset_info:
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features:
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- name: id
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# SynthMT: A Synthetic Benchmark for Automated Microtubule Segmentation
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**Authors:** Mario Koddenbrock*, Justus Westerhoff*, Dominik Fachet, Simone Reber, Felix Gers, Erik Rodner
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*
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**Project Page:** https://datexis.github.io/SynthMT-project-page/
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**Code & Pipeline:** https://github.com/ml-lab-htw/SynthMT
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**Paper:** *Synthetic Data Enables Human-Grade Microtubule Analysis with Foundation Models for Segmentation*
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**Dataset:** https://huggingface.co/datasets/HTW-KI-Werkstatt/SynthMT
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_`*Equal contribution`_
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# 🧬 Overview
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**SynthMT** is a synthetic, expert-validated dataset designed to benchmark segmentation models on *in vitro* microtubule (MT) images visualized in interference reflection microscopy (IRM)–like conditions.
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It provides:
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---
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# 🖼 Example from the Dataset
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<div align="center">
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</tr>
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</table>
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**
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</div>
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---
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# 📦 Dataset Structure
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Each sample in SynthMT contains:
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| Field | Type | Description |
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|---|---|---|
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| `id` | `string` | Unique image identifier |
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| `image` | `Image` | Synthetic IRM-like image, decoded from PNG. Can be converted to a numpy array `(
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| `mask` | `Array3D` | **Stack of instance masks** with shape `(C, 512, 512)` and `uint16` dtype, where `C` = number of instances in the image. Background pixels = 0. Stored in the dataset as a `Sequence(Image())` but can be stacked in memory for in-memory pipelines. |
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---
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# 🧫 Biological Motivation
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Microtubules (MTs) are cytoskeletal filaments essential for intracellular transport, cell motility, and mitotic spindle formation. Measuring MT **count**, **length**, and **curvature** is critical for *in vitro* reconstitution experiments, drug discovery, and mechanistic cell biology.
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---
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# 📥 Installation & Loading
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Install the Hugging Face `datasets` library:
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sample = ds[0]
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# Image as numpy array (H, W, 3)
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img_array = np.array(sample["image"].convert("RGB"))
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# Masks as stacked numpy array (C, H, W)
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---
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# 🔗 Links
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* **Project Page:** [https://datexis.github.io/SynthMT-project-page/](https://datexis.github.io/SynthMT-project-page/)
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* **Dataset:** [https://huggingface.co/datasets/HTW-KI-Werkstatt/SynthMT](https://huggingface.co/datasets/HTW-KI-Werkstatt/SynthMT)
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* **Code & Generation Pipeline:** [https://github.com/ml-lab-htw/SynthMT](https://github.com/ml-lab-htw/SynthMT)
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* **Paper:** [https://www.biorxiv.org/content/10.64898/2026.01.09.698597v2](https://www.biorxiv.org/content/10.64898/2026.01.09.698597v2)
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---
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# 📄 Citation
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```bibtex
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@article{koddenbrock2026synthetic,
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---
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# 🏷 License
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**CC-BY-4.0** - See [LICENSE](LICENSE) for details.
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---
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# 🙏 Acknowledgements
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Our work is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) Project-ID 528483508 - FIP 12.
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We would like to thank Dominik Fachet and Gil Henkin from the Reber lab for providing data,
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and also thank the further study participants Moritz Becker, Nathaniel Boateng, and Miguel Aguilar.
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The Reber lab thanks staff at the Advanced Medical Bioimaging Core Facility (Charité, Berlin) for imaging support
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and the Max Planck Society for funding. Furthermore, we thank Kristian Hildebrand and Chaitanya A. Athale (IISER Pune, India)
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and his lab for helpful discussions
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task_ids:
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- instance-segmentation
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size_categories:
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- 1K<n<10K
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dataset_info:
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features:
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- name: id
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# SynthMT: A Synthetic Benchmark for Automated Microtubule Segmentation
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- **Authors:** Mario Koddenbrock*, Justus Westerhoff*, Dominik Fachet, Simone Reber, Felix Gers, Erik Rodner
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- *Equal contribution
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- **Affiliations:** HTW Berlin, BHT Berlin, MPI for Infection Biology
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- **Project Page:** https://datexis.github.io/SynthMT-project-page/
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- **Code & Pipeline:** https://github.com/ml-lab-htw/SynthMT
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- **Paper:** [*Synthetic Data Enables Human-Grade Microtubule Analysis with Foundation Models for Segmentation*](https://www.biorxiv.org/content/10.64898/2026.01.09.698597v2)
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## 🧬 Overview
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**SynthMT** is a synthetic, expert-validated dataset designed to benchmark segmentation models on *in vitro* microtubule (MT) images visualized in interference reflection microscopy (IRM)–like conditions.
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It provides:
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---
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## 🖼 Example from the Dataset
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<div align="center">
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</tr>
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</table>
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**Overview of SynthMT and example predictions.**
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</div>
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---
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## 📦 Dataset Structure
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Each sample in SynthMT contains:
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| Field | Type | Description |
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|---|---|---|
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| `id` | `string` | Unique image identifier |
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| `image` | `Image` | Synthetic IRM-like image, decoded from PNG. Can be converted to a numpy array `(512, 512, 3)` for in-memory processing. |
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| `mask` | `Array3D` | **Stack of instance masks** with shape `(C, 512, 512)` and `uint16` dtype, where `C` = number of instances in the image. Background pixels = 0. Stored in the dataset as a `Sequence(Image())` but can be stacked in memory for in-memory pipelines. |
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---
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## 🧫 Biological Motivation
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Microtubules (MTs) are cytoskeletal filaments essential for intracellular transport, cell motility, and mitotic spindle formation. Measuring MT **count**, **length**, and **curvature** is critical for *in vitro* reconstitution experiments, drug discovery, and mechanistic cell biology.
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---
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## 📥 Installation & Loading
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Install the Hugging Face `datasets` library:
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sample = ds[0]
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# Image as numpy array (H, W, 3) = (512, 512, 3)
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img_array = np.array(sample["image"].convert("RGB"))
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# Masks as stacked numpy array (C, H, W)
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---
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## 🔗 Links
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* **Project Page:** [https://datexis.github.io/SynthMT-project-page/](https://datexis.github.io/SynthMT-project-page/)
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* **This Dataset:** [https://huggingface.co/datasets/HTW-KI-Werkstatt/SynthMT](https://huggingface.co/datasets/HTW-KI-Werkstatt/SynthMT)
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* **Real IRM in-vitro MT dataset (also used in our paper):** [https://huggingface.co/datasets/HTW-KI-Werkstatt/IRM-in-vitro-microtubules](https://huggingface.co/datasets/HTW-KI-Werkstatt/IRM-in-vitro-microtubules)
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* **Code & Generation Pipeline:** [https://github.com/ml-lab-htw/SynthMT](https://github.com/ml-lab-htw/SynthMT)
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* **Paper:** [https://www.biorxiv.org/content/10.64898/2026.01.09.698597v2](https://www.biorxiv.org/content/10.64898/2026.01.09.698597v2)
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---
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## 📄 Citation
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If you use this dataset, please cite:
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```bibtex
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@article{koddenbrock2026synthetic,
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---
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## 🏷 License
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**CC-BY-4.0** - See [LICENSE](LICENSE) for details.
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---
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## 🙏 Acknowledgements
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Our work is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) Project-ID 528483508 - FIP 12.
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We would like to thank Dominik Fachet and Gil Henkin from the Reber lab for providing data,
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and also thank the further study participants Moritz Becker, Nathaniel Boateng, and Miguel Aguilar.
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The Reber lab thanks staff at the Advanced Medical Bioimaging Core Facility (Charité, Berlin) for imaging support
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and the Max Planck Society for funding. Furthermore, we thank Kristian Hildebrand and Chaitanya A. Athale (IISER Pune, India)
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and his lab for helpful discussions
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