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| language: | |
| - en | |
| pretty_name: "NTv3 annotation dataset - Genomes & reference gene annotations" | |
| tags: | |
| - 𧬠genomics | |
| - π·οΈ gene annotation | |
| - π― benchmark | |
| - β‘ fine-tuning | |
| - π¬ bioinformatics | |
| task_categories: | |
| - other | |
| size_categories: | |
| - n<1K | |
| license: cc-by-nc-4.0 | |
| # NTv3 Annotation Dataset | |
| Genomes and reference gene annotations behind the NTv3 gene-annotation models | |
| ([`NTv3_annot_mammals`](https://huggingface.co/InstaDeepAI/NTv3_annot_mammals), | |
| [`NTv3_annot_plants`](https://huggingface.co/InstaDeepAI/NTv3_annot_plants), | |
| [`NTv3_annot_insects`](https://huggingface.co/InstaDeepAI/NTv3_annot_insects)). It holds two | |
| things: | |
| - **The benchmark**: the 17 test species held out from training (2 mammals, 5 plants, | |
| 10 insects). Each species comes with its complete soft-masked genome, the reference | |
| annotation the benchmark scores against, and the reference's every annotated isoform. | |
| The models' own predictions on these genomes are in each model repository's | |
| `annot_results/` folder. | |
| - **The plant tutorial data**: training and validation genomes with per-base labels, used by | |
| the genome-annotation notebooks of the | |
| [`ntv3`](https://huggingface.co/spaces/InstaDeepAI/ntv3) Space | |
| (03a training, 03b decoding, 03c evaluation). | |
| ## Dataset organization | |
| ``` | |
| InstaDeepAI/NTv3_annot_dataset/ | |
| βββ <clade>/test_species/<assembly>/ # clade = mammals, plants or insects | |
| β βββ genome.fa.gz # soft-masked genome, bgzipped | |
| β βββ genome.fa.gz.fai # samtools faidx index | |
| β βββ genome.fa.gz.gzi # bgzip index | |
| β βββ reference_longest_isoform.gff3.gz | |
| β βββ reference_all_isoforms.gff3.gz | |
| β βββ pseudogenes.bed | |
| βββ plants/ | |
| βββ metadata.tsv # one row per tutorial species | |
| βββ class_counts.json | |
| βββ class_weights.json | |
| βββ train_species/<assembly>/ # genome.fa.gz(.fai, .gzi), labels.bed.gz | |
| βββ val_species/<assembly>/ # same as test species, plus labels.bed.gz, | |
| β # without reference_all_isoforms.gff3.gz | |
| βββ test_species/<assembly>/ # Medicago and Mimulus also carry labels.bed.gz; | |
| # Medicago carries demo_log_probs/ | |
| ``` | |
| Folders are named by assembly, as in the model repositories' `annot_results/` files. | |
| ## Files | |
| - **`genome.fa.gz`**: the soft-masked genome (repeats in lowercase), bgzipped, one record | |
| per sequence, record names cut to the sequence ID. For test species it is the complete | |
| assembly, every contig, with the sequence names of the reference annotation. Tutorial | |
| training and validation genomes keep only the contigs of at least 131,072 bp, the | |
| tutorial's training window. | |
| - **`reference_longest_isoform.gff3.gz`**: the reference the benchmark scores against. | |
| Protein-coding genes only, one transcript per gene (the one with the longest CDS), | |
| `gene`, `mRNA` and `CDS` rows, `ID` and `Parent` attributes only. 1-based closed | |
| coordinates (GFF3). | |
| - **`reference_all_isoforms.gff3.gz`** (test species): the same genes, with every | |
| transcript the source annotation release gives them, in the same format. A prediction | |
| that matches any transcript of a gene counts under the any-isoform rule. When the source | |
| release does not carry a reference transcript's exact CDS, that transcript is kept as | |
| well, so the file is a strict superset of the longest-isoform reference: 171 genes whose | |
| IDs were assigned when the longest isoform was extracted (`agat-gene-*`: 151 immune | |
| receptor gene segments in cow and whale, 15 chloroplast genes, 5 insect genes) and one | |
| transcript in cow (*PEG10*, ID suffixed `.longest_isoform`). | |
| - **`pseudogenes.bed`**: pseudogene regions of the source annotation that do not overlap a | |
| reference CDS. Predicted genes there are ignored when scoring. BED, 0-based half-open. | |
| - **`labels.bed.gz`** (tutorial species): the per-base training labels, one of 15 classes | |
| (`intergenic`, `intron_p0-2`, `CDS_p0-2`, `splice_donor_p0-2`, `splice_acceptor_p0-2`, | |
| `start_codon`, `stop_codon`), as BED runs per strand (`chrom`, `start`, `end`, `class`, | |
| `.`, `strand`), 0-based half-open. | |
| - **`plants/metadata.tsv`**: the tutorial species, their role (`train`, `validation`, | |
| `test`), tier (the tutorial's presets train on tiers 1-2), source, accession and the | |
| chromosome the tutorial evaluates on (`eval_chromosomes`). | |
| - **`plants/class_counts.json`**: per-species base counts of each class. Notebook 03a | |
| derives its loss weights from them, for the genomes it trains on. | |
| - **`plants/class_weights.json`**: those weights precomputed over all the training genomes | |
| (mean inverse class frequency). | |
| - **`demo_log_probs/`** (Medicago): precomputed per-base log-probabilities of | |
| `NTv3_annot_plants` over `NC_053042.1:11,010,048-12,582,912`, decoded in notebook 03b; | |
| `meta.json` records the model, code revision, windows and hardware. | |
| Scores are gene- and CDS-level F1: a predicted gene counts only if its whole CDS structure | |
| matches exactly, predicted genes with less than 201 nt of CDS are ignored, and so are those | |
| in `pseudogenes.bed`. Notebook 03c implements the evaluation. | |
| ## Test species | |
| | Clade | Folder | Species | Contigs | Genes | Transcripts | Genome | Reference annotation | | |
| |---|---|---|---:|---:|---:|---|---| | |
| | mammals | `GCF_002263795.3_ARS-UCD2.0` | *Bos taurus* (cow) | 1,958 | 21,577 | 49,639 | NCBI RefSeq | NCBI RefSeq GCF_002263795.3-RS_2024_12 | | |
| | mammals | `GCF_002288925.2_ASM228892v3` | *Delphinapterus leucas* (beluga whale) | 5,905 | 18,389 | 40,864 | NCBI RefSeq | NCBI RefSeq Annotation Release 101 | | |
| | plants | `GCF_000005505.3_Brachypodium_distachyon_v3.0` | *Brachypodium distachyon* | 11 | 25,477 | 32,269 | NCBI RefSeq | NCBI RefSeq Annotation Release 103 | | |
| | plants | `EcalifornicavarOrangeKingHAP1v1.1` | *Eschscholzia californica* 'Orange King' HAP1 | 6 | 29,562 | 42,970 | Phytozome 828 | Phytozome v1.1 | | |
| | plants | `FmultifloravarABG20040013HAP1v1.1` | *Freycinetia multiflora* ABG20040013 HAP1 | 30 | 28,264 | 38,805 | Phytozome 868 | Phytozome v1.1 | | |
| | plants | `GCF_003473485.1_MtrunA17r5.0-ANR` | *Medicago truncatula* | 42 | 31,794 | 37,660 | NCBI RefSeq | NCBI RefSeq GCF_003473485.1-RS_2025_04 | | |
| | plants | `MguttatusvarIM62v3.1` | *Mimulus guttatus* IM62 | 23 | 25,096 | 31,078 | Phytozome 797 | Phytozome v3.1 | | |
| | insects | `GCF_030269925.1_ASM3026992v2` | *Bombyx mori* | 30 | 13,251 | 22,941 | NCBI RefSeq | NCBI RefSeq GCF_030269925.1-RS_2024_01 | | |
| | insects | `GCF_021464435.1_ULB_Chis1_1.0` | *Cataglyphis hispanica* | 453 | 10,380 | 17,463 | NCBI RefSeq | NCBI RefSeq Annotation Release 100 | | |
| | insects | `GCF_905220415.1_ilColCroc2.1` | *Colias croceus* | 32 | 13,364 | 18,775 | NCBI RefSeq | NCBI RefSeq Annotation Release 100 | | |
| | insects | `GCF_018135715.1_MEX_DaPlex` | *Danaus plexippus* | 66 | 12,669 | 19,058 | NCBI RefSeq | NCBI RefSeq GCF_018135715.1-RS_2023_11 | | |
| | insects | `GCF_905404315.1_ilLepSina1.1` | *Leptidea sinapis* | 49 | 12,276 | 19,563 | NCBI RefSeq | NCBI RefSeq Annotation Release 100 | | |
| | insects | `GCF_905147045.1_ilAglIoxx1.1` | *Nymphalis io* (syn. *Aglais io*) | 41 | 12,024 | 18,680 | NCBI RefSeq | NCBI RefSeq Annotation Release 100 | | |
| | insects | `GCF_907164935.1_iOsmBic2.1` | *Osmia bicornis* | 441 | 11,262 | 18,822 | NCBI RefSeq | NCBI RefSeq Annotation Release 101 | | |
| | insects | `GCF_031307605.1_icTriCast1.1` | *Tribolium castaneum* | 149 | 11,953 | 19,088 | NCBI RefSeq | NCBI RefSeq GCF_031307605.1-RS_2024_04 | | |
| | insects | `GCF_905220365.1_ilVanCard2.1` | *Vanessa cardui* | 36 | 13,070 | 17,975 | NCBI RefSeq | NCBI RefSeq Annotation Release 100 | | |
| | insects | `GCF_012273895.1_Zerene_cesonia_1.1` | *Zerene cesonia* | 275 | 11,791 | 15,989 | NCBI RefSeq | NCBI RefSeq Annotation Release 100 | | |
| *Genes* is the number of reference genes; *Transcripts*, the coding transcripts in | |
| `reference_all_isoforms.gff3.gz`. NCBI genomes are the `_genomic.fna.gz` of the assembly; | |
| the Phytozome genomes are its `softmasked` FASTA (Freycinetia: the 30 scaffolds of | |
| Phytozome 868 concatenated). | |
| ## Plant tutorial species | |
| | Role | Folder | Species | Source | | |
| |---|---|---|---| | |
| | train (tier 1) | `TAIR10` | *Arabidopsis thaliana* | Ensembl Plants (Araport11 genes) | | |
| | train (tier 1) | `GCF_000004075.3_Cucumber_9930_V3` | *Cucumis sativus* | NCBI RefSeq | | |
| | train (tier 1) | `GCF_022201045.2_DVS_A1.0` | *Citrus sinensis* | NCBI RefSeq | | |
| | train (tier 1) | `GCF_019578655.1_ASM1957865v1` | *Ricinus communis* | NCBI RefSeq | | |
| | train (tier 1) | `GCF_020921035.1_ASM2092103v1` | *Rhodamnia argentea* | NCBI RefSeq | | |
| | train (tier 1) | `GCF_005286985.2_Setaria_viridis_v4.0` | *Setaria viridis* | NCBI RefSeq | | |
| | train (tier 1) | `GCF_016808095.1_ASM1680809v1` | *Vigna angularis* | NCBI RefSeq | | |
| | train (tier 1) | `GCF_030704535.1_ASM3070453v1` | *Vitis vinifera* | NCBI RefSeq | | |
| | train (tier 2) | `GCF_031755915.1_ASM3175591v1` | *Ziziphus jujuba* | NCBI RefSeq | | |
| | train (tier 2) | `GCF_018687715.1_C.illinoinensisPawnee_v1` | *Carya illinoinensis* | NCBI RefSeq | | |
| | train (tier 2) | `GCF_036884655.1_Cavendish_Baxijiao_AAA` | *Musa acuminata* | NCBI RefSeq | | |
| | train (tier 2) | `GCF_958298935.1_lpPhrAust1.1` | *Phragmites australis* | NCBI RefSeq | | |
| | train (tier 2) | `IRGSP-1.0` | *Oryza sativa* | Ensembl Plants genome, NCBI RefSeq genes | | |
| | validation | `GCF_000002775.5_P.trichocarpa_v4.1` | *Populus trichocarpa* | NCBI RefSeq | | |
| | validation | `Tlatifoliav1.1` | *Typha latifolia* | JGI (GenBank copy, GCA_059706395.1) | | |
| | test | `GCF_003473485.1_MtrunA17r5.0-ANR` | *Medicago truncatula* | see the test species table | | |
| | test | `MguttatusvarIM62v3.1` | *Mimulus guttatus* IM62 | see the test species table | | |
| `plants/metadata.tsv` also lists tier 3-4 species; their files are not in this dataset. | |
| ## Download | |
| ```python | |
| from huggingface_hub import hf_hub_download, snapshot_download | |
| # One file | |
| path = hf_hub_download( | |
| "InstaDeepAI/NTv3_annot_dataset", | |
| "mammals/test_species/GCF_002288925.2_ASM228892v3/reference_longest_isoform.gff3.gz", | |
| repo_type="dataset", | |
| ) | |
| # One species | |
| local_dir = snapshot_download( | |
| "InstaDeepAI/NTv3_annot_dataset", | |
| repo_type="dataset", | |
| allow_patterns="plants/test_species/GCF_003473485.1_MtrunA17r5.0-ANR/*", | |
| ) | |
| ``` | |
| ## Sources and licence | |
| The files we derived (labels, class statistics, log-probabilities) are | |
| released under CC BY-NC 4.0. Genomes and reference annotations are redistributed from NCBI | |
| RefSeq, Ensembl Plants and JGI Phytozome under their own terms; please cite the original | |
| assemblies and annotations. The three Phytozome genomes are listed as unrestricted: | |
| - *Mimulus guttatus* IM62 (assembly v3.0, annotation v3.1): Lovell et al., *Molecular | |
| Ecology Resources* (2025), https://doi.org/10.1111/1755-0998.70012 | |
| - *Eschscholzia californica* 'Orange King' HAP1 v1.1: RΓΆssner et al., *The Plant Cell* | |
| (2026), https://doi.org/10.1093/plcell/koag039 | |
| - *Freycinetia multiflora* ABG20040013 HAP1 (assembly v1.0, annotation v1.1): JGI Open | |
| Green Genomes, https://doi.org/10.25585/1488189 | |
| ## Citation | |
| Citation to be added on publication. | |