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The JWT signature verification failed. Check the signing key and the algorithm.
Error code:   JWTInvalidSignature
Exception:    InvalidSignatureError
Message:      Signature verification failed
Traceback:    Traceback (most recent call last):
                File "/src/libs/libapi/src/libapi/jwt_token.py", line 286, in validate_jwt
                  decoded = jwt.decode(
                      jwt=token,
                  ...<2 lines>...
                      options=options,
                  )
                File "/usr/local/lib/python3.14/site-packages/jwt/api_jwt.py", line 368, in decode
                  decoded = self.decode_complete(
                      jwt,
                  ...<8 lines>...
                      leeway=leeway,
                  )
                File "/usr/local/lib/python3.14/site-packages/jwt/api_jwt.py", line 265, in decode_complete
                  decoded = self._jws.decode_complete(
                      jwt,
                  ...<3 lines>...
                      detached_payload=detached_payload,
                  )
                File "/usr/local/lib/python3.14/site-packages/jwt/api_jws.py", line 270, in decode_complete
                  self._verify_signature(
                  ~~~~~~~~~~~~~~~~~~~~~~^
                      signing_input,
                      ^^^^^^^^^^^^^^
                  ...<4 lines>...
                      options=merged_options,
                      ^^^^^^^^^^^^^^^^^^^^^^^
                  )
                  ^
                File "/usr/local/lib/python3.14/site-packages/jwt/api_jws.py", line 417, in _verify_signature
                  raise InvalidSignatureError("Signature verification failed")
              jwt.exceptions.InvalidSignatureError: Signature verification failed

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Prophage ACR Screening Pipelines

This directory contains two analysis pipelines for screening Anti-CRISPR (Acr) proteins from prophage genomes.

Directory Structure

Folder Description
double_diff/ Double-divergence screening pipeline: identifies novel Acr candidates that are significantly different from known Acr proteins at both sequence and structure levels
double_similar/ High-confidence Acr identification pipeline: identifies high-confidence Acr candidates that are highly similar to known Acr proteins at both sequence and structure levels

Quick Navigation

  • double_diff — For discovery of Acrs with novel mechanisms. Excludes proteins with sequence homology (qcovs≥30, pident≥30) or structural similarity to known Acrs, retaining "double-divergent" candidates.
  • double_similar — For expansion of known Acr families. Applies stringent filters for sequence (qcovs≥50, pident>50) and structural (TM-score>0.7, prob=1.0) similarity to obtain high-confidence candidates.

Detailed Documentation

Each folder contains a comprehensive README document describing:

  • Screening strategies and workflows
  • Input and output data formats
  • Software tools and parameter settings
  • Screening thresholds and criteria

Click the links above to view detailed documentation for each pipeline.

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