PMCID stringclasses 30
values | sentence stringlengths 1 1.04k | entities listlengths 0 22 |
|---|---|---|
PMC6892818 | Antibody binding was detected using goat anti-rabbit IRDye® 800 (Tebu-bio, Cambs, UK) or goat anti-mouse Ig Alexa Fluor 680 (Thermo Fisher Scientific). | [] |
PMC6892818 | For lysates prepared from 3T3neo/3T3CD40L (effector) and epithelial (target) cell co-cultures, sample loading was corrected/adjusted for epithelial cells according to reactivity with human-specific anti-cytokeratin 18 (CK18) antibody (C8541) (Sigma) and not using antibodies detecting non-phosphorylated intracellular si... | [
{
"end": 32,
"label": "CellLine",
"start": 26,
"text": "3T3neo"
},
{
"end": 41,
"label": "CellLine",
"start": 33,
"text": "3T3CD40L"
}
] |
PMC6892818 | Immunolabelling was visualised using an Odyssey Infra-Red imaging system (LiCor, Cambs, UK). | [] |
PMC6892818 | Inhibitors for AP-1 (NDGA), NF-κB (PDTC), NADPH oxidase (Nox) (DPI) and the antioxidant N-acetyl l-cysteine (NAC) were from Sigma. | [] |
PMC6892818 | Inhibitors for JNK (SP600125), p38 (SB202190) and MEK/ERK (U0126) were from Enzo. | [] |
PMC6892818 | Inhibitors for caspase-8 (z-IETD-FMK), -9 (z-LEHD-FMK), -10 (z-AEVD-FMK) and pan-caspase inhibitor (z-VAD-FMK) were from R&D Systems (supplied by Bio-Techne, Abingdon, UK). | [] |
PMC6892818 | NAC was reconstituted in DR/5% medium and its pH adjusted to 7.0 using 1 M NaOH solution, and was filter-sterilised before use. | [] |
PMC6892818 | All other inhibitors were reconstituted in DMSO (Sigma). | [] |
PMC6892818 | RCC cells were co-cultured with 3T3Neo or 3T3CD40L in the presence of inhibitors for 48 h and apoptosis determined as above. | [
{
"end": 38,
"label": "CellLine",
"start": 32,
"text": "3T3Neo"
},
{
"end": 50,
"label": "CellLine",
"start": 42,
"text": "3T3CD40L"
}
] |
PMC6892818 | Cells treated with DMSO alone (vehicle controls) were included. | [] |
PMC6892818 | Epithelial cells were either (a) cultured in 24-well plates at 5 × 10 cells/well and treated with 10 µg/ml of cross-linked G28-5 mAb or (b) co-cultured at 5 × 10 cells/well with 6 × 10 cells/well growth-arrested 3T3CD40L and 3T3Neo in 24-well plates. | [
{
"end": 220,
"label": "CellLine",
"start": 212,
"text": "3T3CD40L"
},
{
"end": 231,
"label": "CellLine",
"start": 225,
"text": "3T3Neo"
}
] |
PMC6892818 | Culture supernatants were collected at specified time-points post-receptor ligation, and secretion of IL-6, IL-8 and GM-CSF was measured by cytokine-specific ELISA or membrane array-based detection (R&D Systems/Bio-Techne). | [] |
PMC6892818 | For ELISA assays measurements were made spectrophotometrically and for membrane arrays by fluorescence measurements on an Odyssey Infra-Red imaging system (LiCor), as recommended by the manufacturer. | [] |
PMC6892818 | For detection of CD40 expression, cells were cultured in flasks until approximately 80% confluent. | [] |
PMC6892818 | Alternatively, for treatment with cytokines, cells were seeded in 24-well plates at 5 × 10 cells/well and, after overnight incubation, they were treated with fresh medium containing 10 units/ml TNF-α or IFN-γ (R&D Systems/Bio-Techne) for 48 h. Cells were then harvested, washed and re-suspended in FACS buffer. | [] |
PMC6892818 | CD40 expression was detected using PE-conjugated mouse anti-human CD40 antibody and an isotype control IgG1-PE (BD Biosciences, Berks, UK). | [] |
PMC6892818 | In all, 10,000 events were acquired on a Guava EasyCyte flow cytometer and results analysed using EasyCyte software (Millipore, Watford, UK). | [] |
PMC6892818 | For detection of intracellular ROS levels, cells were treated with 1 μM H2DCFDA (Thermo Fisher Scientific) for 30 min, fluorescence measurements (relative fluorescence units, RFU) were taken spectrophotometrically and results expressed as fold increase in RFU, as described in detail previously. | [] |
PMC6892818 | It is noted that when co-cultures were performed, effector (3T3Neo/3T3CD40L) cells were not growth-arrested using MMC to minimise background 3T3 cell-associated fluorescence. | [
{
"end": 66,
"label": "CellLine",
"start": 60,
"text": "3T3Neo"
},
{
"end": 75,
"label": "CellLine",
"start": 67,
"text": "3T3CD40L"
},
{
"end": 144,
"label": "CellLine",
"start": 141,
"text": "3T3"
}
] |
PMC6892818 | Mean values and standard error of the mean (SEM) were used as descriptive statistics. | [] |
PMC6892818 | Two-tailed, paired or non-paired Student’s t-tests were used for evaluation of statistical significance. | [] |
PMC6892818 | For graphical purposes in the figure captions: *p < 0.05, **p < 0.01 and ***p < 0.001, while NS denotes non-significance (p > 0.05). | [] |
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