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PMC1462997
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Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy.
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SB was responsible for deriving CD34 cells from the hESC and culturing macrophages.
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PMC1462997
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Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy.
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JA performed the phenotypic, functional and infection assays on the differentiated macrophages.
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PMC1462997
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Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy.
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DSK provided hES cell protocols and supplied lentiviral vector transduced cells.
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PMC1462997
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Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy.
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RA was responsible for the overall experimental design and implementation of the project.
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PMC1462997
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Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy.
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hES cell derived macrophages support productive HIV-1 infection: Macrophages derived from transduced and nontransduced hES CD34 and fetal liver CD34 cells were infected with macrophage R5-tropic HIV-1 BaL-1 strain at an m.o.i.
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PMC1462997
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Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy.
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of 0.01.
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PMC1462997
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Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy.
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Culture supernatants were collected on different days post infection and assayed for viral p24 antigen by ELISA.
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PMC1462997
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Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy.
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Data is representative of triplicate experiments.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Fibroblasts sculpt the architecture and cellular microenvironments of various tissues.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Here we constructed a spatially resolved atlas of human skin fibroblasts from healthy skin and 23 skin diseases, with comparison to 14 cross-tissue diseases.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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We define six major skin fibroblast subtypes in health and three that are disease-specific.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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We characterize two fibroblast subtypes further as they are conserved across tissues and are immune-related.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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The first, F3: fibroblastic reticular cell-like fibroblast (CCL19CD74HLA-DRA), is a fibroblastic reticular cell-like subtype that is predicted to maintain the superficial perivascular immune niche.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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The second, F6: inflammatory myofibroblasts (IL11MMP1CXCL8IL7R), characterizes early human skin wounds, inflammatory diseases with scarring risk and cancer.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F6: inflammatory myofibroblasts were predicted to recruit neutrophils, monocytes and B cells across multiple human tissues.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Our study provides a harmonized nomenclature for skin fibroblasts in health and disease, contextualized with cross-tissue findings and clinical skin disease profiles.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Fibroblasts are crucial cells for shaping tissue architecture and immune cell niches.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Studying the heterogeneity of fibroblast subtypes has been challenging due to the scarcity of unique surface markers and their tendency to adopt activated phenotypes during in vitro culture.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics technologies have overcome these challenges, enabling the dissection of fibroblast heterogeneity in human tissues.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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While recent studies have described fibroblast states in human skin, they have not spatially resolved their tissue microanatomical location.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Very few, if any, have interrogated fibroblasts in diverse disease conditions in the skin and across human tissues.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Consequently, the fibroblast composition and function in human skin; how it changes across a range of diseases (inflammatory, cancer and fibrosis/scarring); and how these populations relate to other human tissues is still unclear.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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In this study, we integrated published large-scale scRNA-seq datasets of healthy human skin and 23 skin diseases and generated spatial transcriptomics data from two different modalities to construct a high-resolution spatially resolved atlas of more than 350,000 adult human skin fibroblasts.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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We provide a consensus annotation of skin fibroblasts based on gene expression profiles and spatial locations, and contextualize these findings with fibroblast data from other healthy and diseased human tissues.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Our scRNA-seq and spatial datasets resources are freely available for download and interactive data exploration at https://cellatlas.io/studies/skin-fibroblast.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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We re-processed and integrated 2.1 million cells from scRNA-seq data of adult human skin, comprising 32 datasets and 251 donors (Fig. 1a and Supplementary Table 1) using single-cell variational inference (scVI) (Methods).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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After quality control, 357,276 high-quality fibroblasts were selected based on canonical marker gene expression (Fig. 1a and Extended Data Fig. 1a).Fig.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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1Identification of fibroblast subtypes in healthy skin.a, Overview of study methodology, including skin atlas integration to delineate fibroblasts, construction of a healthy/nonlesional reference, mapping of 23 diseases to the healthy reference atlas and downstream analysis for cross-tissue comparison.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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b, Uniform Manifold Approximation and Projection (UMAP) of healthy and nonlesional skin fibroblasts colored by fibroblast subtype.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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DS, dermal sheath; DP, dermal papilla.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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c, Dotplot of marker gene expression for healthy fibroblasts. ‘
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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All’ indicates a marker for a general population, but which contains subtypes.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Supplementary Data Fig. 1a provides additional differentially expressed genes for fibroblast subtypes.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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d, Summary of skin fibroblast subtypes in healthy steady-state tissue.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Illustrations in a and d were partly created using BioRender.com.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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a, Overview of study methodology, including skin atlas integration to delineate fibroblasts, construction of a healthy/nonlesional reference, mapping of 23 diseases to the healthy reference atlas and downstream analysis for cross-tissue comparison.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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b, Uniform Manifold Approximation and Projection (UMAP) of healthy and nonlesional skin fibroblasts colored by fibroblast subtype.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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DS, dermal sheath; DP, dermal papilla.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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c, Dotplot of marker gene expression for healthy fibroblasts. ‘
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
|
All’ indicates a marker for a general population, but which contains subtypes.
|
PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Supplementary Data Fig. 1a provides additional differentially expressed genes for fibroblast subtypes.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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d, Summary of skin fibroblast subtypes in healthy steady-state tissue.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Illustrations in a and d were partly created using BioRender.com.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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In healthy skin, we identified six major fibroblast subtypes based on differential gene expression (Supplementary Data Fig. 1a and Supplementary Table 2) and pathway enrichment analysis (Extended Data Fig. 2a and Methods).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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The six fibroblast subtypes were observed across different covariates (Extended Data Fig. 1b–g and Supplementary Note 1).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Complementary spatial transcriptomic methods validated the presence of each of the six fibroblast subtypes and revealed their distinct microanatomical locations (Fig. 2a–c, Extended Data Figs. 3 and 4 and Supplementary Fig. 2).Fig.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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2Skin fibroblasts occupy unique spatial and functional niches.a, Spatial location of fibroblast subtypes in microenvironments (cell2location abundance predictions (10x Genomics Visium)) in a single section of healthy human skin (left).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Histopathological annotation of tissue microenvironments (right).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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b, Spatial location of fibroblasts at single-cell resolution (10x Genomics Xenium 5000-gene panel) for skin sections from nonlesional skin of atopic dermatitis (noninflamed (left) and noninflamed post-treatment (right)), colored by cell type.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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c, Summary of fibroblast niches: Xenium cell types overlying H&E-stained image (manual approximations).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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a, Spatial location of fibroblast subtypes in microenvironments (cell2location abundance predictions (10x Genomics Visium)) in a single section of healthy human skin (left).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Histopathological annotation of tissue microenvironments (right).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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b, Spatial location of fibroblasts at single-cell resolution (10x Genomics Xenium 5000-gene panel) for skin sections from nonlesional skin of atopic dermatitis (noninflamed (left) and noninflamed post-treatment (right)), colored by cell type.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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c, Summary of fibroblast niches: Xenium cell types overlying H&E-stained image (manual approximations).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Two of the six fibroblast populations (F1: superficial (papillary) and F2: universal (reticular)) were uniformly present throughout skin at different tissue depths.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F1: superficial (papillary) fibroblasts localized adjacent to the skin epithelium in the papillary dermis (Fig. 2b,c) and expressed genes encoding superficial dermal collagens (COL13A1, COL18A1 and COL23A1) and Wnt signaling inhibitors (APCDD1, WIF1 and NKD2) (Fig. 1c).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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A Wnt-mediated synergistic interplay between superficial dermal fibroblasts and basal epithelial cells has been reported to reciprocally maintain cellular identity.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F2: universal (reticular) fibroblasts were located deeper in the skin, interspersed between large collagen fibers in the reticular dermis (Fig. 2b,c).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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This population was characterized by high expression of marker genes of universal PI16 fibroblasts (PI16, CD34 and MFAP5), a fibroblast subtype found in many human tissues and postulated to represent a precursor fibroblast cell state.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Transcription factor activity inference identified KLF5 in F2: universal fibroblasts (Extended Data Fig. 2b), which has been reported to drive the universal Pi16 state.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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As fascial fibroblasts (F_Fascia) are proposed as a potential progenitor cell in mouse skin, we included these cells in an additional integration, identifying that F_Fascia formed a subset of F2: universal (Extended Data Fig. 1i,j and Supplementary Note 2).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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The remaining fibroblast subsets were more focal in localization, being associated with vascular or adnexal structures.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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We thus used hematoxylin and eosin (H&E) staining to illustrate these microenvironments.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F3: fibroblastic reticular cell (FRC)-like fibroblasts were located predominantly in the superficial perivascular region in proximity to immune cells (Fig. 2b and Extended Data Figs. 3a,b and 4a,b).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F3: FRC-like fibroblasts transcriptomically resembled FRCs, which are specialized fibroblasts found in lymphoid organs/structures that maintain immune niches (Extended Data Fig. 1h), expressing genes that attract and compartmentalize immune cells (CCL19, CXCL12 and CH25H), maintain immune cell survival and function (IL33, IL15, TNFSF13B and VCAM1) and enable antigen presentation (CD74 and major histocompatibility complex (MHC)-II molecules).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F2/3: perivascular fibroblasts also localized with immune cells but, unlike F3: FRC-like fibroblasts, were additionally enriched in deep perivascular regions and other sites (Fig. 2a–c and Extended Data Fig. 4b,c).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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A fraction of F2/3: perivascular fibroblasts showed elevated expression of PPARG (Fig. 1c) and pathway analysis suggested a role in adipocyte differentiation (Extended Data Fig. 2a).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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The capability to differentiate into adipocytes is characteristic of the reticular fibroblast (equivalent to F2: universal) lineage.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F2/3: perivascular fibroblasts shared select gene expression with both F2: universal and F3: FRC-like fibroblasts (Fig. 3c).Fig.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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3Prototype meta-learning to identify disease-adapted and disease-specific populations.a, Overview of reference-mapping approach used for integration, where lesional/diseased data were mapped using a pretrained model.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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b, UMAP of scPoli embeddings colored by predicted cell-type labels and relabeled populations after re-clustering.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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c, Dotplot of marker gene expression for disease-adapted and disease-specific fibroblast populations.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Supplementary Data Fig. 1c provides additional differentially expressed genes for disease-associated fibroblast subtypes.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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d, Density of cells in embedding by site status.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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e, Gene expression in F1 and F3 fibroblasts from health and disease, including differentially expressed genes in lesional/diseased states.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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f, Summary of disease-adapted and disease-specific populations.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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g, Feature maps for genes associated with myofibroblast subtypes.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Color bars indicate expression (log1P norm).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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h, PROGENy pathway scores for fibroblasts from lesional and healthy samples.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Illustrations in f were partly created using BioRender.com.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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a, Overview of reference-mapping approach used for integration, where lesional/diseased data were mapped using a pretrained model.
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PMC12479362
|
A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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b, UMAP of scPoli embeddings colored by predicted cell-type labels and relabeled populations after re-clustering.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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c, Dotplot of marker gene expression for disease-adapted and disease-specific fibroblast populations.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Supplementary Data Fig. 1c provides additional differentially expressed genes for disease-associated fibroblast subtypes.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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d, Density of cells in embedding by site status.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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e, Gene expression in F1 and F3 fibroblasts from health and disease, including differentially expressed genes in lesional/diseased states.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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f, Summary of disease-adapted and disease-specific populations.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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g, Feature maps for genes associated with myofibroblast subtypes.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Color bars indicate expression (log1P norm).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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h, PROGENy pathway scores for fibroblasts from lesional and healthy samples.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Illustrations in f were partly created using BioRender.com.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F4: hair follicle-associated fibroblasts (ASPNCOL11A1) encompassed three subclusters that were associated with specific regions of the hair follicle (Fig. 2b,c).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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The first is a well-characterized dermal sheath (DS) population (F4: DS_DPEP1) that wraps around the lower/mid hair follicle (Fig. 2b).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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The second is a novel F4: TNNCOCH subtype, expressing tendon-associated genes (MKX and TNMD) and observed at the isthmus (mid-hair shaft) (Fig. 2b and Extended Data Fig. 4d).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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The third F4: DP_HHIP subtype uniquely expressed dermal papilla marker genes (CORIN, HHIP, RSPO3 and LEF1).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F5: Schwann-like fibroblasts (SCN7A, FMO2, FGFBP2 and OLFML2A) contained two subclusters (F5: NGFR and F5: RAMP1) (Extended Data Fig. 1k).
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F5: RAMP1 fibroblasts were enriched near innervated eccrine glands and expressed genes encoding the receptor complex for the neuropeptide CGRP (Fig. 2b,c and Extended Data Figs. 1l, 3c,d and 4c), suggesting a possible interface with the nervous system.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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F5: NGFR colocalized with Schwann cells, suggesting that they are a nerve-associated population.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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Fibroblasts have been described in the endoneurium and perineurium of nerve fibers from imaging studies, and ‘Schwann-like fibroblasts’ have recently been reported in human skin scRNA-seq data.
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PMC12479362
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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues.
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We confirmed that our six fibroblast subtypes were distinct from Schwann cells and pericytes (Extended Data Fig. 1j,k and Supplementary Note 2).
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