MicPie/unpredictable_studystack-com · Datasets at Hugging Face

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41571226_ards_about_Introduction_Baking__Answer	[Question] Used to make cakes; has less protein; is a safter flour [Answer]	Cake flour	[]	Flashcards about Introduction Baking	Answer	http://www.studystack.com/flashcard-270606	22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json
41571226_ards_about_Introduction_Baking__Answer	[Question] A protein processed from the bones, skin and connective tissue or animals [Answer]	Glatin	[]	Flashcards about Introduction Baking	Answer	http://www.studystack.com/flashcard-270606	22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json
41571226_ards_about_Introduction_Baking__Answer	[Question] Log stretchy strands that trap carbon dioxide given off by yeast. This developed by kneading the dough [Answer]	Guten	[]	Flashcards about Introduction Baking	Answer	http://www.studystack.com/flashcard-270606	22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json
41571226_ards_about_Introduction_Baking__Answer	[Question] Refined from sugar cane or sugar beets, white sugar [Answer]	Ganulated sugar	[]	Flashcards about Introduction Baking	Answer	http://www.studystack.com/flashcard-270606	22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json
41571226_ards_about_Introduction_Baking__Answer	[Question] grease-resistant, nonstick, heatproof paper; for lining pans [Answer]	Parchment paper	[]	Flashcards about Introduction Baking	Answer	http://www.studystack.com/flashcard-270606	22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json
41571226_ards_about_Introduction_Baking__Answer	[Question] Refrigerated cabinet used to control fermentation (growth of dough) [Answer]	Retarder	[]	Flashcards about Introduction Baking	Answer	http://www.studystack.com/flashcard-270606	22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json
41571226_ards_about_Introduction_Baking__Answer	[Question] Secret fact about Ms. Bevins [Answer]	Her favorite color is PINK!	[]	Flashcards about Introduction Baking	Answer	http://www.studystack.com/flashcard-270606	22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] tubehead [Question]	part of the xray unit that contains the xray tube, the high voltage and low voltage transformers and insulating oil	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] PID [Question]	lead lined position indicator device is used to aim the x-ray beam at the film/digital sensor in the patients mouth	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] Extension arm [Question]	wire between the tubehead and the control panel is enclosed in the hollow extension arm, the arm has an important function in positioning the tubehead	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] control panel [Question]	where you adjust the KVP/MA, kind of xray you are taking, area to be exposed	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] power switch/ master switch [Question]	turn machine on and off	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] exposure button [Question]	button to take the xray	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] milliamperage selector [Question]	controls the number of electrons that are produced	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] kilovoltage selector [Question]	used to control the penetrating power of the x-ray beam	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] lead apron [Question]	device used to protect the reproductive and blood forming tissues from scatter radiation	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] thyroid collar [Question]	a flexible led shield that is placed securely around the neck	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] traditional/digital selector [Question]	button to select what kind of xray your going to take: digital , traditional	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] anterior, posterior , bitewing radiograph to be taken [Question]	what area you are going to expose	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] digital sensor [Question]	sensor that is used to take digital radiographs	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] size # 2 film [Question]	x-ray film used to take traditional posterior/ anterior/bitewing xrays	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] size # 1 film [Question]	x-ray film used to take traditional anterior films	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] size # 4 film [Question]	x-ray film used to take traditional occlusal films	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] duplicate film size # 2 [Question]	x-ray film used to take traditional films that need a copy made at the same time	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] equipment used for taking/placement of traditional anterior films [Question]	blue ring, blue bite block,2 bends in rod	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] equipment used for taking / placement of traditional posterior films [Question]	yellow ring, yellow block,one bend in rod	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] equipment used for taking/ placement of traditional bitewing films [Question]	red ring, red block,straight rod	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] equipment used for bisecting when paralelling cant be done, or when traditional ring/rods cant be used [Question]	snap a ray	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] equipment used for taking/placment of digital sensor for posterior xrays [Question]	black ring double side rod holder, yellow block, angled rod	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] equipment used for taking/placment of digital sensor for anterior xrays [Question]	black ring double side rod holder, blue block, angled rod	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] equipment used for taking /placement of digital sensor for bitewing xrays [Question]	black ring single side rod holder, red block, straight rod	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
cf7b7f74_Flashcards_about_xray_unit__Question	[Answer] equipment used for takning/ placement of digital sensor for endodontic xrays or when ring/rods can not be placed ( bisecting ) [Question]	green holders	[]	Flashcards about xray unit	Question	http://www.studystack.com/flashcard-773995	22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json
278e50f4_ards_about_Costofwinning_Vocab__Term	[Definition] strong persuasive influence [Term]	Pressure	[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]	Flashcards about Costofwinning Vocab	Term	http://www.studystack.com/flashcard-1485370	22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json
278e50f4_ards_about_Costofwinning_Vocab__Term	[Definition] a situation in which two people or teams are trying to do better than one another [Term]	rivalry	[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]	Flashcards about Costofwinning Vocab	Term	http://www.studystack.com/flashcard-1485370	22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json
278e50f4_ards_about_Costofwinning_Vocab__Term	[Definition] to do with the body [Term]	physical	[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]	Flashcards about Costofwinning Vocab	Term	http://www.studystack.com/flashcard-1485370	22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json
278e50f4_ards_about_Costofwinning_Vocab__Term	[Definition] strain or pressure [Term]	stress	[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]	Flashcards about Costofwinning Vocab	Term	http://www.studystack.com/flashcard-1485370	22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json
278e50f4_ards_about_Costofwinning_Vocab__Term	[Definition] feeling of worry, nervousness, or suspense [Term]	tension	[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]	Flashcards about Costofwinning Vocab	Term	http://www.studystack.com/flashcard-1485370	22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json
278e50f4_ards_about_Costofwinning_Vocab__Term	[Definition] to cause suffering [Term]	afflict	[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]	Flashcards about Costofwinning Vocab	Term	http://www.studystack.com/flashcard-1485370	22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json
278e50f4_ards_about_Costofwinning_Vocab__Term	[Definition] to want something that belongs to someone else [Term]	covet	[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]	Flashcards about Costofwinning Vocab	Term	http://www.studystack.com/flashcard-1485370	22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json
278e50f4_ards_about_Costofwinning_Vocab__Term	[Definition] something that you think about all the time [Term]	obsession	[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]	Flashcards about Costofwinning Vocab	Term	http://www.studystack.com/flashcard-1485370	22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] The process by which light is absorbed by a molecule and then re emitted at a longer wavelength, producing a particular color. [Question]	What is fluorescence?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] the chemical structure in a fluorescent molecule that absorbs and emits the photons of light. [Question]	What is a fluorophore?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] enhanced green fluorescent protein. has increased fluorescent light output. 35x more brightly. Ser64Thr [Question]	EGFP	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] enhanced cyan fluorescent protein. changed the color from green to cyan. [Question]	ECFP	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] enhanced yellow FP. changed color to yellow. [Question]	EYFP	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] is from the coral Discosoma. It is a tetramer formed of 4 identical subunits of 26kDa. [Question]	DsRed	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] GFP and DsRed [Question]	The 2 parent proteins	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] pRSET in the host bacterium E.Coli strain DH5alpha. [Question]	FP genes were cloned into the plasmid vector...	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] are circular double stranded DNA mol'cs capable of autonomous replication w/in host bacterium. they are not part of the bacterial chromosome. [Question]	plasmids	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] are a vehicle for maintaining and propagating cloned genes in a host organism, such as E.coli. foreign genes can by inserted into a plasmid vector and are rep'd along w/plasmid DNA inside bact. cell. [Question]	vectors	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] the production of multiple identical copies of an organism, cell or gene. if a gene is isolated from genetic material of an organism, and inserted into a vector where identical copies can be rep'd, it is referred to as cloned. [Question]	cloning	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] bacteriophage T7 promotor. This sequence is the binding site for T7 RNA polymerase that will synthesize the mRNA. [Question]	PT7	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] ribosome binding site. Sequence that binds the mRNA to the ribosome. [Question]	RBS	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] vector start codon. This codon codes for methionine, and is the signal for the ribosome to initiate synthesis of the AA chain. [Question]	ATG	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] His-tag. This sequence encodes 6 histidine aa acids in tandem. These will bind to a nickel aff. column for fast and efficient purification of the protein encoded on the cloned gene. [Question]	6xHis	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] 8 aa sequence(DLYDDDDK). that is recognized by an antibody. This provides an alternative method for purifying(and detecting) the protein by aff. chromatography. [Question]	Xpress Epitope Tag	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] aa seq. that is cut by the protease enterokinase. it can be used to remove the preceding aa(met,6 his, DLYDDDDK) that are encoded by the vector. [Question]	EK	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] multiple cloning site. a series of tandem restr.enzy. cutting sites.(BAMHI-HindIII).this is where the gene encoding the protein of interest (DNA frag. containing pRSET and gene) is inserted into vector. [Question]	MCS	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] vector translation stop. The stop codon TAA terminates translation from the mRNA. [Question]	Stop	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] origin of rep. for f1 bacteriophage of e.coli. used in connection w/helper phage(which provides all necessary f1 gene products) to produce single str. copies of the plasmid w/cloned genes(useful for DNA seq.ing of the cloned gene) [Question]	f1 ori	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] the BLA gene that encodes beta-lactamase, the enzy. that degrades the antibiotic ampicillin. In the presence of ampicillin, only bact. containing a plasmid w/amp-resistance gene will live. [Question]	Ampicillin(BLA gene)	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] inhibits the enzymes that form peptide cross-links btwn carbohydrate chains and bacterial cell wall. This weakens the cell wall and lysis eventually occurs. [Question]	Ampicillin	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] an E.coli replication initiation site is inserted here. The DNA polymerases of the host bacterium bind to this site to replicate the vector DNA. [Question]	pUC	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] The MCS of pRSET-B. BAMHI is the site cut by the restriction enzyme BAMHI, where the 5' end of the FP gene was insterted.EcoR1 is the site where the 3' end of the gene was inserted. [Question]	Where were the genes for FP's cloned to?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] to increase the [solute] outside of the cell so when sol.II is added, osmotic pressure draws H2O out hopefully carrying the plasmid. [Question]	The role of glucose in Solution one of alkaline lysis mini plasmid prep?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] EDTA is a chelator. It's main function is to remove Mg2+ and inactivate DNA nucleases for which this ion is cofactor. [Question]	The role of EDTA in solution I of the alkaline lysis mini plasmid prep?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] It is a ribonuclease that will degrade contaminating bacterial RNA. [Question]	The role of RNase in solution I of the alkaline lysis mini plasmid prep?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] To raise the pH, which causes the DNA to denature and the separate strands will ppt from solution. [Question]	The role of NaOH in solution II?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] to lyse the cells by dissolving the cell membrane. [Question]	The role of 1% SDS Page in solution II?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] It makes the solution more neutral. Causes plasmid DNA strands to re-anneal. K+ ions comples w/dodecyl sulfate ions and ppt. [Question]	The role of Kacetate in solution III?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] It is too big and complex to re-anneal under those conditions. [Question]	Why doesn't the chromosomal DNA re-anneal in solution III?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] causes the plasmid DNA to ppt. [Question]	The role of Isopropanol?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] to remove remaining salts and detergents. [Question]	The role of ethanol wash?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] Is measured by the ratio of A260/A280. Greater than 1.6 is adequate. Greater than 1.8 is better. Protein contamination reduces the ratio. [Question]	Purity of Plasmid DNA	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] 260nm [Question]	Nucleotides absorb UV light with a maximum at?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] 1. concentrate DNA or RNA and/or 2. remove detergents, salts and other low MW contaminants. or to change buffer composition. [Question]	The purpose of an alcohol precipitation is to?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] there is a monovalent cation to neutralize the negative charges and permit DNA to aggregate. [Question]	nucleic acids will not precipitate unless...	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] free deoxynucleotides, as they co-precipitate less with NH4 than Na. [Question]	NH4acetate is preferred when one wants to remove..	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] RNA, b/c it is more soluble in ethanol than Na, and efficient ppt of RNA requires large amounts of ethanol than DNA. [Question]	LiCl is commonly used to ppt	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] K+ is used when you want the SDS to ppt as well. SDS is soluble, and KDS or potassium dodecyl sulfate is is insoluble. [Question]	When ppting nucleic acids in the presence of SDS, why use K+?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] Since the polar water molecules orient in concentric shells around the charges, forming hydration spheres. This shields there charge so the attractive forces btwn them is less and they don't come together to form a ppt. [Question]	Why is water an excellent solvent for oppositely charged molecules?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] separates charges so the attractive forces btwn them is less and they don't come together and form a ppt. [Question]	hydration sphere	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] 1.alcohols form Hbonds with water, which reduces the amt of water available to dissolve 2. the capacity of alcohols to shield opp charges and keep them apart is much less than water. [Question]	When alcohol is added to solution, solubility is decreased for two reasons:	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] are nucleases that are produced by bacteria to protect them from viruses that infect bacterial cells, called bacteriophage. They recognize a spec. seq of nucleotides in bacteriophage chrom. and produce a dbl str cut in DNA to prevent replication. [Question]	restriction enzymes	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] By methylating adenosine or cytidine nucleotide in the sequence, which prevents endonuclease from binding. [Question]	How does the bacterium protect its own DNA from being cut by restriction enzymes?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] with the first letter of the genus, followed by the first 2 letters of the species, followed by the strain or serotype if any, and a roman numeral if the bacterium contains more than 1type of res. enzy. [Question]	How are restriction enzymes named?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] This is due to the acidic phosphodiaster groups. [Question]	Why does DNA have a uniformly negative charge?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] it migrates faster b/c it is more compact. plasmid dna is normally supercoiled [Question]	Supercoiled DNA will migrate slower or faster than liner DNA on agarose gel electrophoresis?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] dna uncoils, but remains circular and usually migrates more slowly than linear dna of the same size [Question]	If DNA is nicked, how will this affect its migration down a gel?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] Tris-acetate EDTA (TAE) and Tris-borate-EDTA (TBE). [Question]	The 2 most common buffer systems for agarose gel electrophoresis are:	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] TAE gives a better resolution and is preferred when DNA is to be purified from the gel afterwards. TBE has a better buffering capacity. [Question]	What is the difference between using TAE and TBE as a buffer system?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] bromophenol blue. it will run at about the same position as 300bp [Question]	What dye was used to track the proteins in the electrophoresis gel?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] a fluorescent dye used in our electro gel to help visualize dna. it works by slipping btwn base pairs of dbl str. dna. it fluoresces at 500nm, and will detect down to 50ng of dna. [Question]	Syber safe	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] a powerful mutagen. a fluorescent dye. fluoresces intensely at 560nm when excited by UV light (260-360nm), permitting detection of as little as 20ng dna. [Question]	ethidium bromide	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] cells that have been treated so they are temporarily able to take up DNA. [Question]	competent cells	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] cold CaCl2 treatment of cells that are dividing rapidly, followed by heat shock. [Question]	Method for making competent cells	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] early-mid log phase. [Question]	What cell phase is transformation most successful in?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] it is proposed that dna cross through adhesion zones where there are pores formed by the fusion of the inner and outer mbrn. adhesion zns only form in growing cells. Ca2+ shields - charge on dna and pm, allowing close contact,this may be favored by low T [Question]	How is it believed that the CaCl2 allow dna molecules to cross through the plasma membrane?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] The heat shock could briefly destabilize the membrane so that somehow the dna is allowed to get across. [Question]	how is it believed the heat shock helps with dna crossing through the PM?	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json
1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question	[Answer] a strain of E.coli carrying the gene for T7 RNA polymerase. [Question]	JM109-DE3	[]	Flashcards about UCSD BIBC3 Exam 3	Question	http://www.studystack.com/flashcard-415050	22/1438042990177.43_20150728002310-00217-ip-10-236-191-2_727692401_3.json

41571226_ards_about_Introduction_Baking__Answer

[Question] Used to make cakes; has less protein; is a safter flour [Answer]

Cake flour

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Flashcards about Introduction Baking

Answer

http://www.studystack.com/flashcard-270606

22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json

41571226_ards_about_Introduction_Baking__Answer

[Question] A protein processed from the bones, skin and connective tissue or animals [Answer]

Glatin

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Flashcards about Introduction Baking

Answer

http://www.studystack.com/flashcard-270606

22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json

41571226_ards_about_Introduction_Baking__Answer

[Question] Log stretchy strands that trap carbon dioxide given off by yeast. This developed by kneading the dough [Answer]

Guten

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Flashcards about Introduction Baking

Answer

http://www.studystack.com/flashcard-270606

22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json

41571226_ards_about_Introduction_Baking__Answer

[Question] Refined from sugar cane or sugar beets, white sugar [Answer]

Ganulated sugar

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Flashcards about Introduction Baking

Answer

http://www.studystack.com/flashcard-270606

22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json

41571226_ards_about_Introduction_Baking__Answer

[Question] grease-resistant, nonstick, heatproof paper; for lining pans [Answer]

Parchment paper

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Flashcards about Introduction Baking

Answer

http://www.studystack.com/flashcard-270606

22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json

41571226_ards_about_Introduction_Baking__Answer

[Question] Refrigerated cabinet used to control fermentation (growth of dough) [Answer]

Retarder

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Flashcards about Introduction Baking

Answer

http://www.studystack.com/flashcard-270606

22/1438042990177.43_20150728002310-00274-ip-10-236-191-2_738375923_3.json

41571226_ards_about_Introduction_Baking__Answer

[Question] Secret fact about Ms. Bevins [Answer]

Her favorite color is PINK!

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Flashcards about Introduction Baking

Answer

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] tubehead [Question]

part of the xray unit that contains the xray tube, the high voltage and low voltage transformers and insulating oil

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] PID [Question]

lead lined position indicator device is used to aim the x-ray beam at the film/digital sensor in the patients mouth

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] Extension arm [Question]

wire between the tubehead and the control panel is enclosed in the hollow extension arm, the arm has an important function in positioning the tubehead

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] control panel [Question]

where you adjust the KVP/MA, kind of xray you are taking, area to be exposed

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] power switch/ master switch [Question]

turn machine on and off

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22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json

cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] exposure button [Question]

button to take the xray

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Flashcards about xray unit

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] milliamperage selector [Question]

controls the number of electrons that are produced

[]

Flashcards about xray unit

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] kilovoltage selector [Question]

used to control the penetrating power of the x-ray beam

[]

Flashcards about xray unit

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] lead apron [Question]

device used to protect the reproductive and blood forming tissues from scatter radiation

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] thyroid collar [Question]

a flexible led shield that is placed securely around the neck

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json

cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] traditional/digital selector [Question]

button to select what kind of xray your going to take: digital , traditional

[]

Flashcards about xray unit

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] anterior, posterior , bitewing radiograph to be taken [Question]

what area you are going to expose

[]

Flashcards about xray unit

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] digital sensor [Question]

sensor that is used to take digital radiographs

[]

Flashcards about xray unit

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] size # 2 film [Question]

x-ray film used to take traditional posterior/ anterior/bitewing xrays

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Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json

cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] size # 1 film [Question]

x-ray film used to take traditional anterior films

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] size # 4 film [Question]

x-ray film used to take traditional occlusal films

[]

Flashcards about xray unit

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] duplicate film size # 2 [Question]

x-ray film used to take traditional films that need a copy made at the same time

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json

cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] equipment used for taking/placement of traditional anterior films [Question]

blue ring, blue bite block,2 bends in rod

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json

cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] equipment used for taking / placement of traditional posterior films [Question]

yellow ring, yellow block,one bend in rod

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json

cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] equipment used for taking/ placement of traditional bitewing films [Question]

red ring, red block,straight rod

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] equipment used for bisecting when paralelling cant be done, or when traditional ring/rods cant be used [Question]

snap a ray

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] equipment used for taking/placment of digital sensor for posterior xrays [Question]

black ring double side rod holder, yellow block, angled rod

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

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cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] equipment used for taking/placment of digital sensor for anterior xrays [Question]

black ring double side rod holder, blue block, angled rod

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json

cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] equipment used for taking /placement of digital sensor for bitewing xrays [Question]

black ring single side rod holder, red block, straight rod

[]

Flashcards about xray unit

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http://www.studystack.com/flashcard-773995

22/1438042990177.43_20150728002310-00048-ip-10-236-191-2_735443754_3.json

cf7b7f74_Flashcards_about_xray_unit__Question

[Answer] equipment used for takning/ placement of digital sensor for endodontic xrays or when ring/rods can not be placed ( bisecting ) [Question]

green holders

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Flashcards about xray unit

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278e50f4_ards_about_Costofwinning_Vocab__Term

[Definition] strong persuasive influence [Term]

Pressure

[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]

Flashcards about Costofwinning Vocab

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http://www.studystack.com/flashcard-1485370

22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json

278e50f4_ards_about_Costofwinning_Vocab__Term

[Definition] a situation in which two people or teams are trying to do better than one another [Term]

rivalry

[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]

Flashcards about Costofwinning Vocab

Term

http://www.studystack.com/flashcard-1485370

22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json

278e50f4_ards_about_Costofwinning_Vocab__Term

[Definition] to do with the body [Term]

physical

[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]

Flashcards about Costofwinning Vocab

Term

http://www.studystack.com/flashcard-1485370

22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json

278e50f4_ards_about_Costofwinning_Vocab__Term

[Definition] strain or pressure [Term]

stress

[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]

Flashcards about Costofwinning Vocab

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http://www.studystack.com/flashcard-1485370

22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json

278e50f4_ards_about_Costofwinning_Vocab__Term

[Definition] feeling of worry, nervousness, or suspense [Term]

tension

[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]

Flashcards about Costofwinning Vocab

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http://www.studystack.com/flashcard-1485370

22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json

278e50f4_ards_about_Costofwinning_Vocab__Term

[Definition] to cause suffering [Term]

afflict

[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]

Flashcards about Costofwinning Vocab

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http://www.studystack.com/flashcard-1485370

22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json

278e50f4_ards_about_Costofwinning_Vocab__Term

[Definition] to want something that belongs to someone else [Term]

covet

[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]

Flashcards about Costofwinning Vocab

Term

http://www.studystack.com/flashcard-1485370

22/1438043062635.98_20150728002422-00068-ip-10-236-191-2_742328099_3.json

278e50f4_ards_about_Costofwinning_Vocab__Term

[Definition] something that you think about all the time [Term]

obsession

[ [ "P", "r", "e", "s", "s", "u", "r", "e" ], [ "r", "i", "v", "a", "l", "r", "y" ], [ "p", "h", "y", "s", "i", "c", "a", "l" ], [ "s", "t", "r", "e", "s", "s" ], [ "t", "e", "n", "s", "i", "o", "n" ], [ "a", "f", "f", "l", "i", "c", "t" ], [ "c", "o", "v", "e", "t" ], [ "o", "b", "s", "e", "s", "s", "i", "o", "n" ] ]

Flashcards about Costofwinning Vocab

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] The process by which light is absorbed by a molecule and then re emitted at a longer wavelength, producing a particular color. [Question]

What is fluorescence?

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] the chemical structure in a fluorescent molecule that absorbs and emits the photons of light. [Question]

What is a fluorophore?

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] enhanced green fluorescent protein. has increased fluorescent light output. 35x more brightly. Ser64Thr [Question]

EGFP

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] enhanced cyan fluorescent protein. changed the color from green to cyan. [Question]

ECFP

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] enhanced yellow FP. changed color to yellow. [Question]

EYFP

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] is from the coral Discosoma. It is a tetramer formed of 4 identical subunits of 26kDa. [Question]

DsRed

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] GFP and DsRed [Question]

The 2 parent proteins

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] pRSET in the host bacterium E.Coli strain DH5alpha. [Question]

FP genes were cloned into the plasmid vector...

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] are circular double stranded DNA mol'cs capable of autonomous replication w/in host bacterium. they are not part of the bacterial chromosome. [Question]

plasmids

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] are a vehicle for maintaining and propagating cloned genes in a host organism, such as E.coli. foreign genes can by inserted into a plasmid vector and are rep'd along w/plasmid DNA inside bact. cell. [Question]

vectors

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] the production of multiple identical copies of an organism, cell or gene. if a gene is isolated from genetic material of an organism, and inserted into a vector where identical copies can be rep'd, it is referred to as cloned. [Question]

cloning

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] bacteriophage T7 promotor. This sequence is the binding site for T7 RNA polymerase that will synthesize the mRNA. [Question]

PT7

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] ribosome binding site. Sequence that binds the mRNA to the ribosome. [Question]

RBS

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] vector start codon. This codon codes for methionine, and is the signal for the ribosome to initiate synthesis of the AA chain. [Question]

ATG

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] His-tag. This sequence encodes 6 histidine aa acids in tandem. These will bind to a nickel aff. column for fast and efficient purification of the protein encoded on the cloned gene. [Question]

6xHis

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] 8 aa sequence(DLYDDDDK). that is recognized by an antibody. This provides an alternative method for purifying(and detecting) the protein by aff. chromatography. [Question]

Xpress Epitope Tag

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] aa seq. that is cut by the protease enterokinase. it can be used to remove the preceding aa(met,6 his, DLYDDDDK) that are encoded by the vector. [Question]

EK

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] multiple cloning site. a series of tandem restr.enzy. cutting sites.(BAMHI-HindIII).this is where the gene encoding the protein of interest (DNA frag. containing pRSET and gene) is inserted into vector. [Question]

MCS

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] vector translation stop. The stop codon TAA terminates translation from the mRNA. [Question]

Stop

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] origin of rep. for f1 bacteriophage of e.coli. used in connection w/helper phage(which provides all necessary f1 gene products) to produce single str. copies of the plasmid w/cloned genes(useful for DNA seq.ing of the cloned gene) [Question]

f1 ori

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] the BLA gene that encodes beta-lactamase, the enzy. that degrades the antibiotic ampicillin. In the presence of ampicillin, only bact. containing a plasmid w/amp-resistance gene will live. [Question]

Ampicillin(BLA gene)

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] inhibits the enzymes that form peptide cross-links btwn carbohydrate chains and bacterial cell wall. This weakens the cell wall and lysis eventually occurs. [Question]

Ampicillin

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1b7c5003_hcards_about_UCSD_BIBC3_Exam_3__Question

[Answer] an E.coli replication initiation site is inserted here. The DNA polymerases of the host bacterium bind to this site to replicate the vector DNA. [Question]

pUC

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[Answer] The MCS of pRSET-B. BAMHI is the site cut by the restriction enzyme BAMHI, where the 5' end of the FP gene was insterted.EcoR1 is the site where the 3' end of the gene was inserted. [Question]

Where were the genes for FP's cloned to?

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[Answer] to increase the [solute] outside of the cell so when sol.II is added, osmotic pressure draws H2O out hopefully carrying the plasmid. [Question]

The role of glucose in Solution one of alkaline lysis mini plasmid prep?

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[Answer] EDTA is a chelator. It's main function is to remove Mg2+ and inactivate DNA nucleases for which this ion is cofactor. [Question]

The role of EDTA in solution I of the alkaline lysis mini plasmid prep?

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[Answer] It is a ribonuclease that will degrade contaminating bacterial RNA. [Question]

The role of RNase in solution I of the alkaline lysis mini plasmid prep?

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[Answer] To raise the pH, which causes the DNA to denature and the separate strands will ppt from solution. [Question]

The role of NaOH in solution II?

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[Answer] to lyse the cells by dissolving the cell membrane. [Question]

The role of 1% SDS Page in solution II?

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[Answer] It makes the solution more neutral. Causes plasmid DNA strands to re-anneal. K+ ions comples w/dodecyl sulfate ions and ppt. [Question]

The role of Kacetate in solution III?

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[Answer] It is too big and complex to re-anneal under those conditions. [Question]

Why doesn't the chromosomal DNA re-anneal in solution III?

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[Answer] causes the plasmid DNA to ppt. [Question]

The role of Isopropanol?

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[Answer] to remove remaining salts and detergents. [Question]

The role of ethanol wash?

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[Answer] Is measured by the ratio of A260/A280. Greater than 1.6 is adequate. Greater than 1.8 is better. Protein contamination reduces the ratio. [Question]

Purity of Plasmid DNA

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[Answer] 260nm [Question]

Nucleotides absorb UV light with a maximum at?

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[Answer] 1. concentrate DNA or RNA and/or 2. remove detergents, salts and other low MW contaminants. or to change buffer composition. [Question]

The purpose of an alcohol precipitation is to?

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[Answer] there is a monovalent cation to neutralize the negative charges and permit DNA to aggregate. [Question]

nucleic acids will not precipitate unless...

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[Answer] free deoxynucleotides, as they co-precipitate less with NH4 than Na. [Question]

NH4acetate is preferred when one wants to remove..

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[Answer] RNA, b/c it is more soluble in ethanol than Na, and efficient ppt of RNA requires large amounts of ethanol than DNA. [Question]

LiCl is commonly used to ppt

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[Answer] K+ is used when you want the SDS to ppt as well. SDS is soluble, and KDS or potassium dodecyl sulfate is is insoluble. [Question]

When ppting nucleic acids in the presence of SDS, why use K+?

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[Answer] Since the polar water molecules orient in concentric shells around the charges, forming hydration spheres. This shields there charge so the attractive forces btwn them is less and they don't come together to form a ppt. [Question]

Why is water an excellent solvent for oppositely charged molecules?

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[Answer] separates charges so the attractive forces btwn them is less and they don't come together and form a ppt. [Question]

hydration sphere

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[Answer] 1.alcohols form Hbonds with water, which reduces the amt of water available to dissolve 2. the capacity of alcohols to shield opp charges and keep them apart is much less than water. [Question]

When alcohol is added to solution, solubility is decreased for two reasons:

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[Answer] are nucleases that are produced by bacteria to protect them from viruses that infect bacterial cells, called bacteriophage. They recognize a spec. seq of nucleotides in bacteriophage chrom. and produce a dbl str cut in DNA to prevent replication. [Question]

restriction enzymes

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[Answer] By methylating adenosine or cytidine nucleotide in the sequence, which prevents endonuclease from binding. [Question]

How does the bacterium protect its own DNA from being cut by restriction enzymes?

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[Answer] with the first letter of the genus, followed by the first 2 letters of the species, followed by the strain or serotype if any, and a roman numeral if the bacterium contains more than 1type of res. enzy. [Question]

How are restriction enzymes named?

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[Answer] This is due to the acidic phosphodiaster groups. [Question]

Why does DNA have a uniformly negative charge?

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[Answer] it migrates faster b/c it is more compact. plasmid dna is normally supercoiled [Question]

Supercoiled DNA will migrate slower or faster than liner DNA on agarose gel electrophoresis?

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[Answer] dna uncoils, but remains circular and usually migrates more slowly than linear dna of the same size [Question]

If DNA is nicked, how will this affect its migration down a gel?

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[Answer] Tris-acetate EDTA (TAE) and Tris-borate-EDTA (TBE). [Question]

The 2 most common buffer systems for agarose gel electrophoresis are:

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[Answer] TAE gives a better resolution and is preferred when DNA is to be purified from the gel afterwards. TBE has a better buffering capacity. [Question]

What is the difference between using TAE and TBE as a buffer system?

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[Answer] bromophenol blue. it will run at about the same position as 300bp [Question]

What dye was used to track the proteins in the electrophoresis gel?

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[Answer] a fluorescent dye used in our electro gel to help visualize dna. it works by slipping btwn base pairs of dbl str. dna. it fluoresces at 500nm, and will detect down to 50ng of dna. [Question]

Syber safe

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[Answer] a powerful mutagen. a fluorescent dye. fluoresces intensely at 560nm when excited by UV light (260-360nm), permitting detection of as little as 20ng dna. [Question]

ethidium bromide

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[Answer] cells that have been treated so they are temporarily able to take up DNA. [Question]

competent cells

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[Answer] cold CaCl2 treatment of cells that are dividing rapidly, followed by heat shock. [Question]

Method for making competent cells

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[Answer] early-mid log phase. [Question]

What cell phase is transformation most successful in?

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[Answer] it is proposed that dna cross through adhesion zones where there are pores formed by the fusion of the inner and outer mbrn. adhesion zns only form in growing cells. Ca2+ shields - charge on dna and pm, allowing close contact,this may be favored by low T [Question]

How is it believed that the CaCl2 allow dna molecules to cross through the plasma membrane?

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[Answer] The heat shock could briefly destabilize the membrane so that somehow the dna is allowed to get across. [Question]

how is it believed the heat shock helps with dna crossing through the PM?

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[Answer] a strain of E.coli carrying the gene for T7 RNA polymerase. [Question]

JM109-DE3

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