with the classical initial rate method can measure enzyme initial rates with wide linear ranges, favourable analysis efficiency and practical levels of substrates; it can be applicable to enzyme-coupled reaction curve or enzyme reaction suffering product inhibition.
Taken together, kinetic analysis of enzyme reaction curves under optimized conditions can screen common reversible inhibitors and enzyme mutants; the integration strategy for measuring enzyme activities can quantify serum enzymes and enzyme labels in enzyme-immunoassays to expand the quantifiable ranges, and can be applied to quantify irreversible inhibitors as environmental pollutants; the integration strategy to quantify enzyme substrate can be the second-generation approaches and potentially find wide applications in clinical laboratory medicine. Therefore, these new methodologies for enzymatic analyses based on chemometrics can potentially find their important applications in biomedical sciences.
4. Acknowledgment
This work is supported by the program for New Century Excellent Talent in University (NCET-09), high-technology-program "863" of China (2011AA02A108), National Natural Science Foundation of China (nos. 30200266, 30672009, 81071427), Chongqing Municipal Commission of Sciences and Technology (CQ CSTC2011BA5039), and Chongqing Education Commission (KJ100313).
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