PMCID
string
Title
string
Sentences
string
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
8 Imatinib resistance analysis and drug sensitivity prediction. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
A-C) Boxplots depicting the expression levels of LARP7 among untreated, imatinib-sensitive, and imatinib-resistant GIST patients in GSE132542, GSE51697, and Proteomics (Sun et al.). (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
D-E) Box plot and point plot showing the relationship between the predicted IC50 values of UMI-77 and LARP7 expression in GSE225819. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Box plot displaying the top 10 drugs with predicted IC50 values significantly lower in the LARP7 high-expression group in GSE136755. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Point plot indicating the top 10 drugs whose predicted IC50 values were negatively correlated with LARP7 in GSE136755.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Subsequently, we aimed to predict drugs that target LARP7 based on the extensive data from GDSC_v2.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In GSE225819, the IC50 values of UMI-77, a Myeloid cell leukemia-1 (Mcl-1) inhibitor, were significantly lower in the LARP7 high-expression group (Fig. 8D) and exhibited a significant negative correlation with LARP7 expression (Fig. 8E).
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In GSE136755, we identified 74 drugs that demonstrated potential efficacy in LARP7 high-expression group (Fig. 8F, 8G and Table S7).
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Notably, Teniposide (a topoisomerase II inhibitor), Carmustine (a DNA alkylator/crosslinker), and GSK2578215A (an LRRK2 inhibitor) exhibited the most promising results in our predictive analysis.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
These findings suggest that LARP7 may be implicated in the imatinib resistance observed in GIST patients, and several drugs have the potential to address this challenge.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Through qRT-PCR and western blotting detection of four pairs of cancer and para-cancerous tissues from GIST patients, it was confirmed that LARP7 was up-regulated in GIST tumor tissues at the clinical level (Fig. 9A and B).
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
To validate the role of LARP7 in promoting GIST, knockdown experiments were conducted in vitro in GIST cancer cell lines.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Results showed that all three siRNAs demonstrated effective LARP7 knockdown at the mRNA (Fig. 9C) and protein levels (Fig. 9D) in GIST-T1 and GIST-882 cells.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The siLARP7–2 exhibited the best effect and was used in subsequent experiments.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In the CCK8 assay, the GIST-T1 and GIST-882 cells with inhibited LARP7 expression exhibited a reduced growth pattern compared to the scrambled vector group (Fig. 9E).
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The wound healing assay displayed a slower healing rate in the LARP7 knockdown groups (Fig. 9F).
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
These results confirmed that LARP7 down-regulation significantly reduced GIST-T1 and GIST-882 cell proliferation and migration.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Fig. 9The role of LARP7 in promoting cell proliferation and migration in GIST-T1 and GIST-882 cells. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
A-B) The expression of LARP7 in four para-cancerous tissues and paired GISTs evaluated using qRT-PCR and western blot analysis. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
GIST-T1 and GIST-882 cells with LARP7 knockdown via three siRNAs were assessed using qRT-PCR, with LARP7 mRNA expression normalized to GAPDH levels. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
GIST-T1 and GIST-882 cells subjected to either control or siLARP7 were harvested to evaluate LARP7 expression through western blot analysis. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
CCK8 assays were performed on GIST-T1 and GIST-882 cells transfected with either control or siLARP7–2 at 24, 48, 72, and 96 h. (F) Wound healing assays were conducted to compare cell migration between the LARP7-inhibited group and the control group, with evaluations conducted 48 h post-transfection in both GIST-T1 and GIST-882 cells. *:
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.Fig.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
9 The role of LARP7 in promoting cell proliferation and migration in GIST-T1 and GIST-882 cells. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
A-B) The expression of LARP7 in four para-cancerous tissues and paired GISTs evaluated using qRT-PCR and western blot analysis. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
GIST-T1 and GIST-882 cells with LARP7 knockdown via three siRNAs were assessed using qRT-PCR, with LARP7 mRNA expression normalized to GAPDH levels. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
GIST-T1 and GIST-882 cells subjected to either control or siLARP7 were harvested to evaluate LARP7 expression through western blot analysis. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
CCK8 assays were performed on GIST-T1 and GIST-882 cells transfected with either control or siLARP7–2 at 24, 48, 72, and 96 h. (F) Wound healing assays were conducted to compare cell migration between the LARP7-inhibited group and the control group, with evaluations conducted 48 h post-transfection in both GIST-T1 and GIST-882 cells. *:
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
To explore the unknown factors in GIST tumorigenesis, we combined transcriptional RNA-seq data and proteomic data to screen for potential key genes.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
After filtering based on their significance in relation to PFS, we identified 35 up-regulated DEGs/DEPs.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Notably, upon examining the background of these genes, we found that many were associated with epigenetic regulation, including ANP32E, CDH11, MSI2, and SYMD4.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
As previously mentioned, a group of transcription factors and chromatin remodeling factors were found to be essential for sustaining GIST proliferation and KIT expression.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Inhibition of MOZ and Menin-MLL complexes resulted in decreased GIST cell proliferation by disrupting interactions with transcriptional and chromatin regulators, such as DOT1 L .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Furthermore, G protein-coupled receptor 20 (GPR20) has recently been identified as a non-tyrosine kinase target in GIST , which was also noted in our results.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
La ribonucleoprotein 7 (LARP7), a member of the La-related protein family, encoded a protein that binds to 7SK RNA.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
This protein was a key component of the 7SK small nuclear ribonucleoprotein (snRNP) complex, which critical in regulating RNA polymerase II pausing by negatively modulating the PTEF-b complex.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In breast cancer, BRCA1/BARD1 catalyzed the K48 polyubiquitination of LARP7, thereby promoting the G2/M transition and the DNA damage response following ionizing radiation .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
LARP7 has been identified as a potential tumor suppressor in gastric and follicular thyroid cancers .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Conversely, under the regulation of HOGA1, LARP7 facilitated the progression of pancreatic ductal adenocarcinoma .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In cooperating with TRIM28, LARP7 inhibited endothelial-to-mesenchymal transition and valvulogenesis .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Additionally, LARP7 can enhance SIRT1 deacetylase activity, which contributed to the slowing of cellular aging .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
LARP7 also involved in regulating mRNA stability and translation processes.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
A deficiency in LARP7 has been linked to impaired telomere maintenance in Alazami syndrome .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
However, the role of LARP7 in the pathogenesis of GIST remained to be fully elucidated.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Our study provided the first evidence that LARP7 functioned as an oncogene in GIST and was significantly associated with the GIST prognosis.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Functional enrichment analysis revealed a significant up-regulation of genes involved in interferon signaling, negative regulation of viral processes, expression of IFN-induced genes, antigen processing and presentation, T cell-mediated cytotoxicity, and other related pathways.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Notably, these functions have not been identified in any cancer types, and only the 7SK snRNP complex has been previously implicated in the process of HIV-1 latency .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Recently, GBP1, the sole DEGs/DEPs identified between high and low LARP7 expression groups in our results, has been reported to play a role in guarding against pathogen infection , promoting tumorigenesis , and contributing to drug resistance .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
These findings further suggest that LARP7, in conjunction with GBP1, may participate in gastrointestinal resistance to viral infection.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The interaction between LARP7 and GBP1 will be further explored in subsequent studies.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
A single-cell RNA-seq analysis of intra- and peri‑tumor tissues from GIST patients demonstrated that T cells constitute the largest proportion of immune cells in the microenvironment .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Our findings indicated that GISTs with high LARP7 expression exhibited increasing infiltration of CD8+ T cells.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Additionally, predictive analysis of cytokine pathway status revealed that the IFN1, IFNG, and IL27 signaling pathways were activated in high LARP7 expression groups.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Furthermore, we observed that the correlation between IFN-related pathway activation and LARP7 expression was particularly pronounced within a small subset of GIST patients, as illustrated in Fig. 6B.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The detection of LARP7 aided in further stratifying patients with GIST, and the characteristics of this small subset of patients warrant detailed investigation.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In our network analysis, we predicted that H19 or LINC00665 targeted hsa-miR-138–5p, which negatively regulated LARP7 expression in GIST.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Extensive research has shown that the lncRNA H19 promotes cancer progression, metastasis, and drug resistance , a function also attributed to the lncRNA LINC00665 .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The hsa-miR-138–5p was associated with cancer cell proliferation and drug resistance across various cancers, including prostate cancer , colorectal cancer , cervical cancer , and renal cell carcinoma .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Notably, the LINC00665/hsa-miR-138–5p/SIN3A axis has been shown to modulate the progression of colorectal cancer .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
While not directly related to cancer, the interaction between H19 and hsa-miR-138–5p has been reported in rat models of severe acute pancreatitis .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
These lncRNAs and miRNA played crucial roles in epigenetic regulation by targeting key transcription factors and chromatin regulators, and their relationship with LARP7 will be experimentally validated in future studies.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In the RBP-mRNA network, LARP7 was targeted by IGF2BP2 and YTHDC1, suggesting that LARP7 may be regulated through N(6)-methyladenosine (m6A) modification.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
SCAF4 and SCAF8, function as anti-terminators, suppressed early mRNA termination .
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The relationship between these RBPs and LARP7 also presented a compelling narrative regarding mRNA metabolism.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Although we found that LARP7 was significantly up-regulated in imatinib-resistant GISTs at the transcriptional level, further analysis and experiments are necessary to elucidate the role of LARP7 in drug resistance.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Predictive drugs such as UMI-77 (an established BH3-mimetic for MCL-1), Carmustine (an alkylating agent), and other agents were identified, which may assist in the treatment of GIST.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
However, our study was limited by a small sample size and a lack of survival data.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Future research utilizing advanced technologies will be essential to fully characterize the role of LARP7 in GIST pathophysiology.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Four paired cancer and para-cancerous tissues from GIST patients validated LARP7 expression was up-regulated in tumor.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Both the CCK8 and wound healing assays demonstrated that the knockdown of LARP7 inhibited the proliferation and migration of GIST-T1 and GIST-882 cells.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
We have demonstrated the cancer-promoting function of LARP7 in clinical samples and in vitro experiments, but our sample size was relatively small and further experiments were pending.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Samples containing prognostic information were still being collected.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In vivo experiments and specific experiments related to epigenetics were ongoing.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
LARP7 served as a link between risk factors such as viral infections and the dysfunction of epigenetic mechanisms in GIST.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
In summary, the detection of LARP7 expression in GIST holds promise for early-stage diagnosis, ongoing surveillance for potential recurrence, and the prediction of therapy response.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
GISTs characterized by high expression of LARP7 exhibited aggressive clinicopathological features, leading to an increased risk of relapse in patients following treatment.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
This study provided valuable insights for the further exploration of personalized therapeutic strategies for GIST.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
This study protocol was granted approval by the Ethics Committee of Sichuan Provincial People's Hospital (Approval Number: 2024–226), adhering to the principles of the Declaration of Helsinki.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Each patient provided written informed consent and approved the utilization of their tissues for research purposes.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Figure S1.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Functional enrichment results of down-regulated DEGs/DEPs. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Functional enrichment network of 281 commonly down-regulated DEGs/DEPs using ClueGO. (
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Point plot displaying the top 35 prognosis-related genes in the down-regulated group.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The point color indicates the absolute value of log2(fold change) for each gene/protein.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The point size reflects the value of -log10(p value).
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The bar plot in the right panel represents the relationship with PFS for each protein.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Heng Zheng: Writing – review & editing, Writing – original draft, Visualization, Validation, Software, Project administration, Methodology, Formal analysis, Data curation, Conceptualization.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
Yong Pan: Writing – review & editing, Writing – original draft, Validation, Software, Project administration, Methodology, Formal analysis, Data curation, Conceptualization.
PMC11867541
Transcriptome-proteome integration analysis identifies elevated expression of LARP7 promoting the tumorigenesis and development of gastrointestinal stromal tumors
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
Liposarcomas (LPSs) are common soft-tissue sarcoma subtypes.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
There is an unmet clinical need for targeting LPS-subtype-specific highly expressed genes.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
We investigated RNA sequence data from a large clinical LPS sample series and an in silico transcriptome database consisting of 201 tissue types.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
We discovered that the PDE3A gene is highly expressed in the myxoid LPS subtype.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
In addition, PDE3A served as a drug target for PDE3A modulators in LPS cell lines, warranting further studies toward its usage in clinical therapy.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
Liposarcomas (LPSs) are a heterogeneous group of malignancies that arise from adipose tissue.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
Although LPSs are among the most common soft-tissue sarcoma subtypes, precision medicine treatments are not currently available.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
To discover LPS-subtype-specific therapy targets, we investigated RNA sequenced transcriptomes of 131 clinical LPS tissue samples and compared the data with a transcriptome database that contained 20,218 samples from 95 healthy tissues and 106 cancerous tissue types.
PMC10669966
PDE3A Is a Highly Expressed Therapy Target in Myxoid Liposarcoma
The identified genes were referred to the NCATS BioPlanet library with Enrichr to analyze upregulated signaling pathways.