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library(ggpubr) work_dir = "/Volumes/mahamid/Dorothy/Pipeline_after_CNN/20230516_new_analysis_for_paper/analysis_output/" setwd(work_dir) ctr_data_read <- na.omit(read.csv(file = "memb_norm_control_memb_actin_dist_5_nm_bin_noupper_averaged.csv")) ind_data_read <- na.omit(read.csv(file = "memb_norm_induced_memb_actin_d...
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replintegrate <- function(means, sds) { sig_p <- estimate_tau(means, sds) var_t <- sds^2 + sig_p^2 var_est <- 1/sum(1 / var_t) mean_est <- sum(means / var_t)*var_est sd_est <- sqrt(var_est) return(list(mean_est = mean_est, sd_est = sd_est, sig_p = sig_p)) } replintegrate_batch <- function(mean_mat, sd_mat)...
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LISP Interpreter Run [[[[[ A LISP expression that asserts that it itself is unprovable! Let g(x): x -> (is-unprovable (value-of (('x)('x)))) Then (is-unprovable (value-of (('g)('g)))) asserts that it itself is not a theorem! ]]]]] define (g x) let (L x y) cons x cons y nil [Makes x and y into list.] (L...
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```{r data} tt <- "F:/promec/Animesh/RawRead/MSMS.tab" vals<-read.table(tt,comment.char = "#",sep="\t") ``` ```{r plot} plot(vals$V8,vals$V5) hist(log2(vals$V8)) ``` ```{r dist} n=1000 valSam=sample(vals$V8,n) hist(log2(valSam)) valsdist=dist(valSam,valSam,method = "euclidean") valH=hist(abs(valsdist[...
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#source https://raw.githubusercontent.com/animesh/compbio_tutorials/main/scripts/06_scRNseq_two_lines_from_fastq_to_count_matrix.Rmd #mamba create -n kb-python python=3.7 #conda activate kb-python #pip install kb-python gget ffq #kb ref -i index.idx -g t2g.txt -f1 transcriptome.fa $(gget ref --ftp -w dna,gtf ho...
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#C:\Users\animeshs>R-4.4.0\bin\Rscript.exe "drive\My Drive\scripts\uniprotIDmap.r" L:\promec\Animesh\Motif\uniprot_sprot.motif.found.txt L:\promec\Animesh\Motif\GOeu.tsv #setup#### #https://www.uniprot.org/uniprotkb?query=%28reviewed%3Atrue%29+AND+%28taxonomy_id%3A2759%29 #download.file("https://rest.uniprot.org/unipr...
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library(ggpubr) work_dir = "/Volumes/mahamid/Dorothy/Pipeline_after_CNN/20230527_new_analysis/analysis_output/" setwd(work_dir) ctr_data_read <- na.omit(read.csv(file = "memb_norm_control_memb_actin_dist_20_nm_bin_noupper_averaged.csv")) ind_data_read <- na.omit(read.csv(file = "memb_norm_induced_memb_actin_dist_20_nm...
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plot_to_results <- function(n_bins, plot_df, region_range = 0:1) { cutoffs <- (0:n_bins) / n_bins results_df <- matrix(0, nrow=n_bins*length(region_range),6) for(region in region_range) { for(bin in 1:n_bins) { in_bin <- which(plot_df$berg_weight <= cutoffs[bin+1] & plot_df$berg_weight >= cutoffs[bin] &...
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--- title: "Visualization stable fold changes of IFN-related genes" author: "Raquel Garza" output: html_document: df_print: paged --- ### This markdown produces supplemental figure 5A, to validate the region-specific (SN and PUT) IFN response. - Load needed libraries - Load relevant clean data - Load the heat...
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library(Matrix) library(spacexr) library(doParallel) library(ggplot2) library(geometry) source('~/Documents/MIT/Research/Rafalab/Projects/slideseq/Cell Demixing/ContentStructure/CSIDE/analysis/personal_utils.R') #datadir <- '../RCTD/data/SpatialRNA/Puck_210605/24' datadir <- '../RCTD/data/SpatialRNA/Puck_210605/James_R...
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# "c:\Program Files\R\R-4.5.1\bin\Rscript.exe" combineCSV.r "F:\promec\HF\Lars\2025\251204_Maren_Gemma\251203_3kda_A9_dda.raw.centroid.MGF.csv" "F:\promec\HF\Lars\2025\251204_Maren_Gemma\251203_3kda_A3_dda.raw.centroid.MGF.csv" "F:\promec\HF\Lars\2025\251204_Maren_Gemma\251203_1kda_A7_dda.raw.centroid.MGF.csv" "F:\pro...
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df <- read.csv("Fig.4d.csv") # Blank layer to divide mapping from not mapping data2 <- data.frame(final_clusters_renamed = rep("MC0.0", 2000),supertype_name = rep("Sst_Gaba_0", 2000), final_clusters_palette = rep("gray", 2000)) data <- rbind(df, data2) data$final_clusters_renamed <- factor(data$final_clusters_rena...
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--- title: "rustSeqMap" author: "ani" date: "12/2/2020" output: html_document source: https://github.com/jperkel/gb_read --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` ```{r} library(tidyverse) ``` Given a path (no terminating separator, eg '~' or '~/tmp', NOT '~/tmp/'), file base name (eg '...
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#git checkout ebbdd3bf84fdd40e6abd7c353fc101fadcedc87c diffExprTestT.r #Rscript.exe diffExprTestT.r L:\promec\TIMSTOF\LARS\2024\240319_Nicola\combined\txt\proteinGroups.txt L:\promec\TIMSTOF\LARS\2024\240319_Nicola\combined\txt\GroupsRem.txt Bio Rem Intensity. WT 0.1 1 0.05 #Rscript codonUsageSelect.r "L:\promec\TIMSTO...
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# ..\R-4.5.0\bin\Rscript.exe .\combineTxt.r "L:\promec\TIMSTOF\LARS\2023\230217_Caroline\mRNA\Omego_T1_vs_Control_T1_mRNA_diff_expr_CPM1.txt" "L:\promec\TIMSTOF\LARS\2023\230217_Caroline\mRNA\Omego_T3_vs_Control_T3_mRNA_diff_expr_CPM1.txt" "L:\promec\TIMSTOF\LARS\2023\230217_Caroline\mRNA\Omego_T6_vs_Control_T6_mRNA_di...
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#Rscript motifSeqAlign.r L:\promec\Animesh\Motif\uniprot_sprot.motif.found.seq.txt "Enolase " #setup#### args = commandArgs(trailingOnly=TRUE) print(paste("supplied argument(s):", length(args))) inpF <- args[1] #perl motif.pl uniprot_sprot.fasta "[RK][FWY][ALVI][GALVI][RK]" | awk -F '\t' '$3!=""' > uniprot_sprot.motif...
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df <- read.csv("Fig.4b.csv") # Blank layer to divide mapping from not mapping data2 <- data.frame(final_clusters_renamed = rep("MC0.0", 2000),supertype_name = rep("Sst_Gaba_0", 2000), final_clusters_palette = rep("gray", 2000)) data <- rbind(df, data2) data$final_clusters_renamed <- factor(data$final_clusters_rena...
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#..\..\R-4.5.0\bin\Rscript proteinGroupsVSN.r "L:\promec\TIMSTOF\LARS\2026\260518_Sonali\DIANNv2P2.63.260612_140833.64.highacc\report.pg_matrix.tsv" "F..promec.TIMSTOF.LARS.2026.260518_Sonali.260518_Sonali_" #install.packages(c("BiocManager"),repos="http://cran.us.r-project.org",lib=.libPaths()) #BiocManager::install(c...
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test_that(".is_discrete() works", { expect_equal(.is_discrete(1:3), FALSE) expect_equal(.is_discrete(10:9, n_unique = 2), TRUE) expect_equal(.is_discrete(c(TRUE, FALSE), n_unique = 3L), TRUE) expect_equal(.is_discrete(c("a", "b"), n_unique = 3L), TRUE) expect_equal(.is_discrete(factor(c("a", "b")), n_unique =...
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# ------------------------------------------------------------------------------ # Title: Plotting TF Weights for Target Gene (Meis1) # Author: Yiran Song # Date: March 18, 2025 # Description: # This script loads gene regulatory network (GRN) output from Commot # and visualizes the weight distribution of transcription...
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#!/usr/bin/Rscript --vanilla ################################################################################## # # MIT License # # Copyright (c) 2025 Kevin Rockenbach, Agnieszka Golicz # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation file...
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#!/usr/bin/env Rscript ##import packages library(readxl) library(tidyverse) library(DESeq2) library(ggfortify) library(ggplot2) library(pheatmap) library(sva) library(biomaRt) library(DescTools) library(ComplexHeatmap) library(circlize) # set working dir to the folder containing this script setwd(dirname(rstudioap...
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#' @import dplyr #' @import ggplot2 #' @import scales #' @importFrom magrittr %>% #suppressPackageStartupMessages({ # library(dplyr) # library(ggplot2) #}) `%>%` <- magrittr::`%>%` args <- commandArgs(trailingOnly=TRUE) workingDir <- args[1] print(paste0("Plotting for ",workingDir)) name = rev(unlist(strsplit(wor...
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library(Matrix) library(ggplot2) library(STutility) library(shiny) library(imager) library(raster) library(rgdal) library(spacexr) ###CONSTANTS IM_DIR <- '../RCTD/data/Images/Plaque/210715/Processed/' ALIGN_DIR <- '../RCTD/data/Images/Plaque/210715/AlignmentResults/' ####START CREATE PUCK IMAGES datadir <- '../RCTD/dat...
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#!/usr/bin/env Rscript # Differential expression analysis using homoscedastic two-sample t-tests. # # Preprocessing matches run_limma.R for fair comparison: # 1. DGEList for integrated count + library-size bookkeeping # 2. Low-count filtering via edgeR::filterByExpr() # 3. TMM normalization via edgeR::calcNormFac...
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library(tidyverse) library(pheatmap) setwd("C:/Users/u0171728/Downloads") df <- read.csv("fe-wp-dataset-120.csv", check.names = FALSE) rownames(df) <- make.unique(df$gene_id) df_expr <- df %>% select(-gene_id, -id) aggregate_celltypes <- function(prefix, df) { cols <- grep(paste0("^", prefix), colnames(df), ...
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#' Extractor Functions #' #' Functions to extract SHAP values, feature values, the baseline, #' or SHAP interactions from a "(m)shapviz" object. #' #' @name extractors #' @param object Object to extract something. #' @param ... Currently unused. #' @returns #' - `get_shap_values()` returns the matrix of SHAP values, ...
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# # collect mappability info in a given chrom or range # process.map.values <- function(map.values, strand, step, read.len, read.left.edge, threshold.fraction) { do.shift = (strand == '-' && read.left.edge) || (strand == '+' && !read.left.edge) if (do.shift) map.values = c(rep(0, read.len - 1), map.values[1...
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library(data.table) library(mclust) input_path <- "PATH_TO_INPUT/samap_leiden_clusters.csv" output_path <- "PATH_TO_OUTPUT/samap_ARI.txt" df <- read.csv(input_path, header = TRUE) rownames(df) <- make.unique(as.character(df$X)) celltype_label <- "celltype" batch_label <- "batch" percent_extract <- 0.8 nbiters <- 20...
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#..\R-4.5.0\bin\Rscript.exe geneGroupsQC.r "Z:/Download/report.gg_matrix.tsv" 1 4 3 #setup#### args = commandArgs(trailingOnly=TRUE) print(paste("supplied argument(s):", length(args))) inpF <- args[1] #inpF<-"Z:/Download/report.gg_matrix.tsv" #inpD<- gsub("[^[:alnum:]]+", ".", dirname(inpF)) geneC <- args[2] #geneC<-"1...
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#' Correlation plots #' #' #' #' @param df_filtered the filtered protein dataset #' @return Distribution maps for top 3 most correlated proteins, and correlation values #' @export correlation_plot <- function(df, target_name, type = 'pearson', location, limit, upperL = 2, lowerL = -2, col...
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## By Anoushka Joglekar 2019. Edited 03/2020 #' @importFrom magrittr %>% #' @import dplyr `%>%` <- magrittr::`%>%` args <- commandArgs(trailingOnly=TRUE) print("Reading in file, this might take a while") all_info <- data.table::fread(args[1]) if(ncol(all_info) == 9){ all_info <- all_info[,c(1:4,6)] colnames(all_i...
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```{r data} #setwd('L:/Elite/LARS/2014/desember/christiano/serum/') #file='W2H.txt' setwd('L:/Elite/LARS/2014/desember/christiano/plantaris/') file='W2C.txt' data <- read.table(file, sep = '\t', header = T) ``` ```{r http://stackoverflow.com/questions/17924828/differences-in-heatmap-clustering-defaults-in-r-he...
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library(ijtiff) my_dir <- '/Users/dcable/Documents/MIT/Research/Rafalab/Projects/slideseq/Cell Demixing/ContentStructure/RCTD/data/Images' im_ind <- 5 mtif <- read_tif(file.path(my_dir,paste0('HCR/Composite',im_ind,'.tif'))) atif <- read_tif(file.path(my_dir,paste0('HCR/c2_i',im_ind,'.tif'))) if(im_ind == 1) { purk_t...
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###-- Linear Models, basic functionality -- weights included. ## From John Maindonald : roller <- data.frame( weight = c(1.9, 3.1, 3.3, 4.8, 5.3, 6.1, 6.4, 7.6, 9.8, 12.4), depression = c( 2, 1, 5, 5, 20, 20, 23, 10, 30, 25)) roller.lmu <- lm(weight~depression, data=roller) roller....
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# ..\R-4.5.0\bin\Rscript.exe .\combineXlsx.r "L:\promec\TIMSTOF\LARS\2023\230217_Caroline\combined\txt\proteinGroups.txtLFQ.intensity.110Omego1Cntr1h00.050.5InfBiotTestBH.xlsx" "L:\promec\TIMSTOF\LARS\2023\230217_Caroline\combined\txt\proteinGroups.txtLFQ.intensity.110Omego3Cntr3h00.050.5InfBiotTestBH.xlsx" "L:\promec\...
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--- #title: "Expression GO" output: html_document runtime: shiny --- ```{r data,echo=F} lPep=100 nPep=10000 nTot=lPep*nPep data<-runif(nTot) #hist(sample(data,100000,replace=T),breaks=nPep) sPep=rnorm(lPep) n=1 i=1 while(n<=lPep){ print(n) print(i) sPep[c(seq(n,n+i-1))]=sample(data,i,replace=T) n=n+i i=i+1 }...
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# ------------------------------------------------------------------------------ # Title: Preparation of Cytospace Input Files from Seurat Objects # Author: Yiran Song # Date: March 18, 2025 # Description: # This script generates input files for Cytospace from Seurat objects. # It processes single-cell and spatial tra...
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#Rscript diffExprQC.r "L:\promec\TIMSTOF\LARS\2023\230310 ChunMei\combined\txt\proteinGroups.txtIntensity.0.110.05BioRemoveGroups.txtAMHCtTestBH.combined.xlsx" Log2MedianChange "L:\promec\TIMSTOF\LARS\2023\230310 ChunMei\combined\txt\Exosome and ion channel proteins.csv" "Exosome..1276." #setup#### args = commandArgs(t...
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```{r setup, include=FALSE} knitr::opts_chunk$set(include = FALSE) #plot par(mfrow=c(1,2)) #library/packages .libPaths( c( .libPaths(), "C:/Users/animeshs/GD/R-4.0.1/library") ) .libPaths() setwd("C:/Users/animeshs/GD/scripts") getwd() chkrVector<-c(22.39459,20.48316,21.87155,NA,20.34495) chkrDF<-as.data.frame(chkrVect...
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library(Matrix) library(spacexr) library(doParallel) library(ggplot2) #source('~/Documents/MIT/Research/Rafalab/Projects/slideseq/Cell Demixing/ContentStructure/RCTD/PersonalFiles/private_utils.R') #source('~/Documents/MIT/Research/Rafalab/Projects/slideseq/Cell Demixing/ContentStructure/RCTD/de.R') #source('~/Document...
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#!/usr/bin/Rscript --vanilla ################################################################################## # # MIT License # # Copyright (c) 2025 Kevin Rockenbach, Agnieszka Golicz # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation file...
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#!/usr/bin/Rscript --vanilla ################################################################################## # # MIT License # # Copyright (c) 2025 Kevin Rockenbach, Agnieszka Golicz # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation file...
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--- source: "https://towardsdatascience.com/ten-random-useful-things-in-r-that-you-might-not-know-about-54b2044a3868" title: "Check" author: "" date: "" output: html_document: code_folding: "hide" params: name: value: n1 choices: - D - C - R years_of_study: input: slider min:...
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# ========================================================== # 01_scan_adni_files.R # Purpose: Scan all ADNI CSV/XLS/XLSX files and find relevant variables # ========================================================== library(tidyverse) library(readxl) library(openxlsx) library(janitor) library(stringr) # -...
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```{r data} fileP<-"L:\\promec\\Animesh\\Lymphoma\\" fileN<-"Sel66.txt" lym<-read.table(paste0(fileP, fileN),row.names = 1, header=T) summary(lym) fileC<-"Code.txt" code<-read.table(paste0(fileP, fileC),row.names = 1, header=T) summary(code) #install.packages('quantable') library(quantable) y<-lym y=robustscale(y) y$da...
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#Rscript metaboGroups.r DR_MOHAN_300S_METABOLOMICS_ANALYSIS_NEGATIVE_MODE.xlsx DR_MOHAN_300S_METABOLOMICS_ANALYSIS_POSITIVE_MODE.xlsx #setup#### args = commandArgs(trailingOnly=TRUE) print(paste("supplied argument(s):", length(args))) print(args) options(nwarnings = 1000000) warnings() #data#### inpFN<-args[1] inpFP<-a...
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#' Computes cell type profiles in a scRNA-seq dataset #' #' Computes averaged normalized expression (summing to 1) for #' all cells within a cell type #' #' @param raw.data a Digital Gene Expression matrix, with gene names as rownames and #' single cells as columns (barcodes for colnames) #' @param cell_types a named l...
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context("Gene Set Co-Regulation Analysis") test_that("GESECA: is reproducible", { data("exampleExpressionMatrix") data("examplePathways") set.seed(1) gr1 <- geseca(pathways=examplePathways, E=exampleExpressionMatrix) set.seed(1) gr2 <- geseca(pathways=examplePathways, E=exampleExpressionMatri...
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R
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#!/usr/bin/Rscript --vanilla ################################################################################## # # MIT License # # Copyright (c) 2025 Kevin Rockenbach, Agnieszka Golicz # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation file...
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#!/usr/bin/Rscript --vanilla ################################################################################## # # MIT License # # Copyright (c) 2025 Kevin Rockenbach, Agnieszka Golicz # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation file...
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--- output: pdf_document: default html_document: keep_md: true --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = T, warning = F, message = F, cache = T, results = 'hide') ``` # Platform Effect Prediction (between single-nucleus and single-cell RNA-seq) ```{r libraries} library(spacexr) library(M...
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library(spacexr) library(Matrix) library(ggplot2) library(devtools) source('~/Documents/MIT/Research/Rafalab/Projects/slideseq/Cell Demixing/ContentStructure/CSIDE/analysis/helper_functions/testes_helper.R') load_all() datadir <- '../RCTD/data/SpatialRNA/Testes' puck <- readRDS(file.path(datadir,'puck.rds')) ### END PR...
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library(knockoff) library(data.table) library(glmnet) `+` <- function(e1, e2) { if (is.character(e1) | is.character(e2)) { paste0(e1, e2) } else { base::`+`(e1, e2) } } source('/home/users/peymanhk/PROJECT_2/Simulated/GOI/KnockoffScreen_updated.R') prt_result = '/home/users/peymanhk/PROJE...
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#' Generate matrices for clustering analysis #' #' @description Converts the counts for isoforms #' into matrices clustered either by cell-type #' or by barcode. #' @seealso \code{\link{IsoQuant}} #' @param isoQuantOutDir output directory of IsoQuant #' function. Defaults to IsoQuantOutput #' @param groupBy String indi...
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#' Get single-cell barcodes from long read files #' #' @description This is an R-wrapper for a python function. It parallelizes the reading #' and processing of fastq.gz files, and uses a sliding window approach to #' identify cell barcodes, assign cluster labels and output useful statistics #' in one .csv file and one...
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#8/10/18: fixed a bug for the enr_se column options(echo=FALSE); param <- commandArgs(trailingOnly=T) annotfile = eval(paste(text=param[1])) resultfile = eval(paste(text=param[2])) outfile = eval(paste(text=param[3])) if (length(param)!=3) { cat("\nError: three arguments must be supplied.\n") cat("ARG1 : the ...
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#c:/Users/animeshs/R/bin/Rscript.exe proteinGroupsTtestCombine.r L:/promec/TIMSTOF/LARS/2021/November/SIGRID/combined/txtNoNQd/ BiotTestBH.csv args = commandArgs(trailingOnly=TRUE) print(paste("supplied argument(s):", length(args))) print(args) if (length(args) != 5) {stop("\n\nNeeds THREE arguments, the full path of t...
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#..\R-4.4.0\bin\Rscript.exe markVolcanoXlsx.r L:\promec\TIMSTOF\LARS\2024\240207_Deo\combined\txt\ proteinGroups.txtLFQ.intensity.16 0.110.05BioRemGroupsG.txttTestBH.csv MLH1 PMS1 PMS2 #Overall plot be smaller #Bigger fonts #Only top-30 interactors should be named of which, every graph should have the protein itself in...
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#' Collapse SHAP values #' #' This function sums up SHAP values (or SHAP interaction values) of feature groups. #' Typical application: SHAP values have been generated by a model with one or multiple #' one-hot encoded variables, but the explanations should be done using the #' original factor. #' #' @param S Either a ...
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#!/usr/bin/Rscript --vanilla ################################################################################## # # MIT License # # Copyright (c) 2025 Kevin Rockenbach, Agnieszka Golicz # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation file...
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source('gatc.r') summary(t(gi.id49175990.gatc)) t(gi.id49175990.gatc)[1,1] hist(t(gi.id49175990.gatc)) gd=read.table('gatcdist.txt') hist(gd) hist(gd$V1) (gd$V1) hist(gd$V1) gd=read.table('gatcdist.txt') hist(gd$V1) hist(gd$V1,breaks=100) hist(gd$V1,breaks=1000) hist(gd$V1,breaks=10000) summary(gd$V1) hist(gd$V1,breaks...
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################################################### ### chunk number 1: ################################################### library(grDevices) library(grid) ps.options(pointsize=12) options(width=60) ################################################### ### chunk number 2: fig1 ###########################...
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#!/usr/bin/Rscript --vanilla ################################################################################## # # MIT License # # Copyright (c) 2025 Kevin Rockenbach, Agnieszka Golicz # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation file...
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--- output: pdf_document: default html_document: keep_md: true --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = T, warning = F, message = F, cache = T, results = 'hide') ``` # Platform Effect Prediction (between single-nucleus and single-cell RNA-seq) ```{r libraries} library(RCTD) library(Matr...
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--- output: github_document --- <!-- README.md is generated from README.Rmd. Please edit that file --> ```{r, include = FALSE} knitr::opts_chunk$set( collapse = TRUE, comment = "#>", fig.path = "man/figures/README-", out.width = "100%" ) ``` ## scisorseqr - a comparative analysis of alternative splicing patt...
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R
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#' Combine all available information per read to a flat file #' @aliases LongReadInfo #' @description Function to concatenate all the information per read, i.e #' gene-name, cellular barcode, UMI, cell-type information, and isoform #' information into one file. Isoform information includes a string separated list of #...
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# Load f1 <- readRDS("Extend Fig.6f_Conserved_clusters_MN.RDS") f2 <- readRDS("Extend Fig.6f_Differences_MeanExpression_of_finalClustersMarkers.RDS") f3 <- readRDS("Extend Fig.6f_HausdorffDistance_q50.RDS") f4 <- readRDS("Extend Fig.6f_Number_DEGs_finalClusters_Atlas.RDS") f5 <- readRDS("Extend Fig.6f_Number_DEGs_...
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#!/usr/bin/Rscript --vanilla ################################################################################## # # MIT License # # Copyright (c) 2025 Kevin Rockenbach, Agnieszka Golicz # # Permission is hereby granted, free of charge, to any person obtaining a copy # of this software and associated documentation file...
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#' @importFrom magrittr %>% #' @import parallel #' @import dplyr #' @export `%>%` <- magrittr::`%>%` args <- commandArgs(trailingOnly=TRUE) ## arguments : AllInfo file, groupingFactor, nThreads, threshold allInfo <- data.table::fread(args[1],sep="\t") if(ncol(allInfo) == 11){ allInfo <- allInfo[,c(1:4,9)] } ...
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cphyll <- read.table("chlorophyll.txt") nodule <- read.table("nodule.txt") wsf <- read.table("weight_sf.txt") wrf <- read.table("weight_rf.txt") boxplot(nodule) boxplot(nodule) boxplot(wsf) boxplot(wrf) wilcox.test(cphyll$Uninoculated,cphyll$GN25) wilcox.test(nodule$Uninoculated,nodule$GN25) wilcox.test(w...
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#https://github.com/Noble-Lab/casanovo/releases/tag/v4.0.0 #casanovo sequence -o 230508_IRD_37TWTL.mztab desk/IRD/230508_IRD_37TWTL.mgf #wget https://github.com/Noble-Lab/casanovo/releases/download/v4.0.0/casanovo_nontryptic.ckpt #casanovo sequence -o 230508_IRD_37GWTL.mztab desk/IRD/230508_IRD_37GWTL.mgf --model cas...
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# ========================================================== # 03_select_adnimerge2_core_candidates.R # Purpose: Find ADNIMERGE2 tables useful for the clinical core dataset # ========================================================== library(tidyverse) library(readxl) library(openxlsx) library(janitor) librar...
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--- output: pdf_document: default html_document: keep_md: true --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = T, warning = F, message = F, cache = T, results = 'hide') ``` # CSIDE on the Slide-seq KP tumor (immune cell-dependent DE) ### Load in CSIDE Results and calculate significant genes `...
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# Load necessary library for string manipulation library(dplyr) # Define the function normalize_and_aggregate <- function(dataframe) { # Rename columns to correct issues colnames(dataframe) <- colnames(dataframe) %>% gsub("X1.cLISI", "cLISI", .) %>% gsub("kBET.Accept.Rate", "kBET", .) %>% gsub("True...
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# Requires: dplyr, ggplot2, ggrepel # install.packages(c("dplyr","ggplot2","ggrepel")) aseManhattan_top20 <- function(input, statvalue = c("pvalue", "qvalue"), annotation, gws = 0.05, filename = ...
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# Generated by using Rcpp::compileAttributes() -> do not edit by hand # Generator token: 10BE3573-1514-4C36-9D1C-5A225CD40393 calcGseaStatCumulativeBatch <- function(stats, gseaParam, pathwayScores, pathwaysSizes, iterations, seed, scoreType) { .Call('_fgsea_calcGseaStatCumulativeBatch', PACKAGE = 'fgsea', stats, ...
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#https://github.com/animesh/love-actually-network library(dplyr) library(stringr) library(tidyr) raw <- readLines("matrix_97_draft.txt") lines <- data_frame(raw = raw) %>% filter(raw != "", !str_detect(raw, "(song)")) %>% mutate(is_scene = str_detect(raw, " Scene "), scene = cumsum(is_scene)) %>% filter(...
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#simulates doublets for testing on scRNA and snRNA cerebellum reference. library(RCTD) library(Matrix) DropViz <- T if(DropViz) { test_reference <- readRDS("Data/Reference/DropVizCerAnnotated/scRefSubsampled1000.RDS") common_cell_types = c("Astrocytes", "Bergmann", "Endothelial", "Fibroblast", "Golgi", "Granule", "...
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S <- cbind( x = c(0.1, 0.1, 0.1), `age low` = c(0.2, -0.1, 0.1), `age mid` = c(0, 0.2, -0.2), `age high` = c(1, -1, 0) ) collapse <- list(age = c("age low", "age mid", "age high")) out <- collapse_shap(S, collapse) test_that("collapse_shap work", { expect_equal(colnames(out), c("x", "age")) expect_equal(ou...
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--- title: "Farmand et al 2026 - NeuroFit RCT Blood-Based Biomarker Analysis Examples" output: html_document --- ```{r setup, message=FALSE, warning=FALSE} library(readxl) library(dplyr) library(tidyr) library(nlme) library(emmeans) library(ggplot2) ``` ## 1. Import dataset ```{r import-data} df <- read_excel("Farma...
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#!/usr/bin/env Rscript ## kernlab regression using nifti files ## James Cole 24/09/2019 rm(list = ls()) args <- commandArgs(trailingOnly = TRUE) ## test if there are two argument: if not, return an error if (length(args) < 6) { stop("Six arguments must be supplied (brainageR directory full path; list of GM nifti file...
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```{r setup} inpD <-"L:/OneDrive - NTNU/PROMEC01/IDH1/" .libPaths(c(paste0(inpD,"/R/")),.libPaths()) .libPaths() #data thr<-20 selThr=0.3 selThrFC=0.5 hdr<-gsub("[^[:alnum:] ]", "",inpD) hdr<-gsub(" ", "",hdr) setwd(inpD) getwd() ``` ```{r library} install.packages("BiocManager") install.packages("scal...
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suppressPackageStartupMessages({ library(Seurat) library(conos) library(ggplot2) }) unintegrated <- readRDS("PATH_TO_INPUT/unintegrated.rds") species_levels <- sort(unique(as.character(unintegrated$species))) letters_map <- setNames(letters[seq_along(species_levels)], species_levels) unintegrated$species <- unn...
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library(Matrix) library(ggplot2) library(STutility) library(shiny) library(imager) library(raster) library(rgdal) library(spacexr) ###CONSTANTS IM_DIR <- '../RCTD/data/Images/Plaque/210715/Processed/' ALIGN_DIR <- '../RCTD/data/Images/Plaque/210715/AlignmentResults/' ####START CREATE PUCK IMAGES datadir <- '../RCTD/dat...
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#!/usr/bin/env Rscript suppressPackageStartupMessages({ library(data.table) }) setwd('/Users/doq/RCode') load('preprocessed_data.RData') # Match core preprocessing used in analysisCode.R alldata[, binary_group := fifelse(grepl('ASD', group, ignore.case = TRUE), 'ASD', 'TD')] alldata[, group_verbal := fcase( is.n...
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#..\R\bin\Rscript.exe proteinSequenceProperties.r #setup#### #https://github.com/dosorio/Peptides/ #install.packages("Peptides", dependencies=TRUE) #https://github.com/jedick/canprot #install.packages("canprot", dependencies=TRUE) #http://chnosz.net/canprot/doc/canprot.html #proteins <- c("LYSC_CHICK", "RNAS1_BOVIN", "...
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#!/usr/bin/env Rscript library(optparse) ref_reselectpc <- function(pcadir, ref) { pcafiles <- unlist(strsplit(list.files(pcadir,pattern=".pc.txt"),".pc.txt")) for(j in 1:length(pcafiles)) { pcadf <- read.table(paste0(pcadir,"/",pcafiles[j],".pc.txt"), h=T, as.is=T) rownames(pcadf) <- paste0(pcadf$chr,"_",pcad...
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#' Perform differential splicing analysis #' @aliases DiffIsoTest #' @description This function allows you to test alternative #' splicing differences between any two groups #' based on the full splice-form, exons, #' the transcription start site (TSS) or end site (polyA). #' @param configFile File in a .tsv format wit...
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Synergy check ======================================================== ```{r load file} #syn=read.csv('L:/Elite/kamila/FA-UDC-IM-M-6.csv') #syn=read.csv('L:/Elite/kamila/flufenamic acid for Animesh.csv') #syn=read.csv('L:/Elite/kamila/UDCIMrawLR5.csv') #syn=read.csv('L:/Elite/kamila/UDCIMraw.csv') #syn=read.cs...
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# Set environment language Sys.setenv(LANG = "en") # Set working directory setwd("D:/project/ndreamer_plot/") # Load necessary libraries library(ggplot2) library(dplyr) library(tidyr) library(scales) # Define a function to create the plot generate_plot <- function(input_df) { # Rename columns to correct issues c...
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library(Seurat) library(SeuratObject) library(Matrix) library(data.table) library(lisi) source('.../benchmark_script/evaluation/ARI_LISI/evaluation_ARI_LISI_R_F.R') pre_dir <- "PATH_TO_PRE_DIR/" save_dir <- "PATH_TO_SAVE_DIR/" temp_dir <- paste0(save_dir, "/temp_files/") dir.create(temp_dir, showWarnings = FALSE,...
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gene_list1<-c('FTH1', 'PCDH9-AS2', 'FTL', 'P2RY14', 'IPO9-AS1', 'ANGPT2', 'FN1', 'SHOC1', 'PDE8A', 'HHIP', 'LINC02899', 'TIMP3', 'B3GNT5', 'ENO4', 'PPP1R9A-AS1', 'SLC7A14-AS1', 'PDE7B-AS1', 'TMEM165', 'RFX4', 'CFTR', 'CTNNA3', 'BACH1', 'LINC02930', 'ABCB1', 'FGF1', 'CPB2', 'IL18', 'TGFB1', 'MARCKSL1', 'CCK', 'HIF1A-AS3...
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#Everything Is Correlated -> https://www.gwern.net/Everything #Common statistical tests are linear models (or: how to teach stats) -> https://lindeloev.github.io/tests-as-linear/ #books: #rmarkdown->https://bookdown.org/yihui/rmarkdown/ #plot->https://plotly-r.com/introduction.html#data-visualization-best-practices , h...
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solveOLS<-function(S,B, solution, constrain = T){ D<-t(S)%*%S d<-t(S)%*%B norm_factor <- norm(D,"2") D <- D / norm_factor d <- d / norm_factor epsilon <- 1e-7; D <- D + epsilon * diag(length(d)) A<-cbind(diag(dim(S)[2])) bzero<-c(rep(0,dim(S)[2])) if(constrain) { A_const = t(rbind(1,A)) b_con...
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df <- read.csv("Extend Fig.9a,b,c,d.csv") df <- df %>% mutate(Category = ifelse(subclass_bootstrapping_probability >= 0.9, ">=0.9", "<0.9")) %>% mutate(SubclassClean_Other = ifelse(subclass_name %in% c("052 Pvalb Gaba","053 Sst Gaba"), subclass_name, "Other")) percentage_data <- df %>% group_by(Major, Category) %>% s...
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# ============================================================ # Helper functions for Perturb-seq module-score analysis # (minimal subset extracted from original functions.R) # ============================================================ #' Count cells expressing a gene above a threshold #' @param object Seurat object...
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#' Extract isoform information from aligned reads #' #' @description This function follows the alignment function. #' It is a wrapper for several scripts that impose checks and #' balances on the mapping quality, consensus nature of splice #' sites, and annotated start and end sites per transcript. #' @seealso \code{\l...
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#"c:\Program Files\R\R-4.5.2\bin\Rscript.exe" diffExprTestCor.r "L:\promec\TIMSTOF\LARS\2026\260518_Sonali\DIANNv2P2.63.260612_140833.64.highacc\report.pg_matrix.tsv" "L:\promec\TIMSTOF\LARS\2026\260518_Sonali\DIANNv2P2.63.260612_140833.64.highacc\Groups.txt" "F..promec.TIMSTOF.LARS.2026.260518_Sonali.260518_Sonali_" "...
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#' Returns a list of Reactome pathways for given Entrez gene IDs #' @param genes Entrez IDs of query genes. #' @return A list of vectors with gene sets. #' @export #' @examples #' data(exampleRanks) #' pathways <- reactomePathways(names(exampleRanks)) reactomePathways <- function(genes) { stopifnot(requireNamespace...