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b21d103ae73ec62db9b5c29693a6c885fc487433d8b826a86940e1548f6b6004
Rust
1,542
45
//! enclone // Copyright (c) 2021 10x Genomics, Inc. All rights reserved. #![deny(missing_docs)] use crate::core::barcode_fate::BarcodeFate; use crate::core::enclone_structs::BarcodeFates; use itertools::zip_eq; use vector_utils::erase_if_iter; pub(crate) mod allele; pub(crate) mod graph_filter; pub(crate) mod info; ...
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Rust
1,588
50
//! Utilities for finding groups of FASTQ files on disk. #![deny(missing_docs)] pub mod bcl2fastq; pub mod bcl_processor; pub mod fastq_dir; pub mod sbx; use serde::{Deserialize, Serialize}; use std::collections::HashSet; /// This enum stores the lane information associated with a fastq file: #[derive(Deserialize, S...
0c5a7c6df039b0036df3101488ae1f9a46ec6a1160d87b8928be070a134a2b5a
Rust
1,590
45
use anyhow::Result; use polars::prelude::*; use std::{fs::File, io::BufWriter}; use crate::enrich::EnrichmentResult; fn build_sgrna_dataframe( sgrna_names: &[String], gene_names: &[String], adj_var: &[f64], sgrna_results: &EnrichmentResult, ) -> Result<DataFrame, PolarsError> { df!( "sgrna...
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Rust
1,631
59
#![deny(missing_docs)] use crate::{FloatAsInt, Percent}; use anyhow::{Result, anyhow, bail}; use serde_json::{Number, Value}; pub trait TryFromValue { fn try_from_value(value: &Value) -> Result<Option<Self>> where Self: Sized; } impl TryFromValue for Number { fn try_from_value(value: &Value) -> R...
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Rust
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48
#![expect(missing_docs)] use crate::reference::probe_set_reference::TargetSetFile; use crate::types::TargetingMethod; use martian_derive::martian_filetype; use martian_filetypes::json_file::JsonFormat; use serde::{Deserialize, Serialize}; martian_filetype!(_TargetPanelSummaryFile, "tps"); pub type TargetPanelSummaryFo...
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Rust
1,660
44
#![expect(missing_docs)] use crate::IntoExitCode; use crate::utils::AllArgs; use anyhow::{Context, Result}; use std::process::{Command, ExitCode}; /// Currently we capture all the arguments and pass them through to /// the old shell wrapper code for mkfastq. /// (full Rust-ification is an early work in progress for no...
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Rust
1,717
58
#![expect(missing_docs)] use crate::types::GenomeName; use anyhow::Result; use barcode::{Barcode, barcode_string}; use itertools::Itertools; use martian_filetypes::LazyFileTypeIO; use martian_filetypes::tabular_file::CsvFileNoHeader; use metric::TxHashSet; use serde::{Deserialize, Serialize}; /// Filtered barcodes CSV...
1d79e015fc62fff8a7b7d1dd4ece95df3be6f9de4120c02e1fc5ba8edd8544d9
Rust
1,748
56
//! Martian stage MERGE_METRICS_MAPS //! Merge metrics JSON files. #![deny(missing_docs)] use anyhow::Result; use cr_types::MetricsFile; use itertools::Itertools; use martian::{MartianMain, MartianRover}; use martian_derive::{MartianStruct, make_mro}; use martian_filetypes::FileTypeRead; use metric::JsonReporter; use ...
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Rust
1,775
51
#![expect(missing_docs)] use crate::reference::feature_reference::FeatureReference; use anyhow::{Result, bail}; use std::collections::HashMap; use std::iter::zip; /// Given an array of integer counts, one per feature. /// Return the proportions, normalized within each feature type. pub fn compute_feature_dist(raw_coun...
89ef8dd0a632fbb164bff705f22c2050ca884d7ea9e5c64a5c231ffffc9d01b6
Rust
1,782
64
use anyhow::Result; use ndarray::s; use polars::frame::DataFrame; use crate::{ aggregation::compute_aggregation, cli::SgrnaColumns, enrich::EnrichmentResult, io::{get_string_column, to_ndarray, write_gene_frame, write_hit_list, Screenviz}, utils::{config::Configuration, logging::Logger}, }; pub fn...
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Rust
1,796
64
#![deny(missing_docs)] use anyhow::Result; use serde::de::Visitor; use serde::{Deserialize, Deserializer, de}; use std::convert::TryFrom; use std::fmt::Formatter; #[derive(Debug)] pub(super) enum NumberOrStr { Number(u64), Str(String), } impl TryFrom<NumberOrStr> for usize { type Error = de::value::Error;...
63933a893085f3405a6ed1f2bef17b950d54a24db0b30283c045fb66faceea47
Rust
1,798
60
use anyhow::Result; use crate::{ aggregation::compute_aggregation, cli::SgrnaColumns, enrich::EnrichmentResult, io::{GeneFrame, HitList, Screenviz, SimpleFrame}, utils::{config::Configuration, logging::Logger}, }; pub fn run_aggregation( frame: &SimpleFrame, columns: SgrnaColumns, conf...
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Rust
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61
//! A thread-safe, fixed-size pool of T. //! This type can be used to provide reusable allocations that can be passed //! between threads, such as in-memory buffers. //! //! The pool will never allocate more than the specified number of members, //! and requesting a member from the pool will block until one is availabl...
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Rust
1,805
51
//! Martian stage EXTRACT_SINGLE_CHEMISTRY. //! This is a utility stage to extract a single pre-declared library type from //! the map of chemistries produced by DETECT_CHEMISTRY. If no library type is //! provided, the stage asserts that only a single chemistry is present and //! returns it. //! //! This is a shim to ...
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Rust
1,815
50
// Copyright (c) 2021 10X Genomics, Inc. All rights reserved. #![deny(missing_docs)] use crate::core::defs::ExactClonotype; // ▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓ pub(crate) fn delete_weaks( exacts: &[usize], exact_clonotypes: &[ExactClonotype], ...
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Rust
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51
//! Martian stage CREATE_MULTI_GRAPH //! Create the multiplexing sample graph. //! This stage requires information from both the multi config CSV and chemistry detection. #![deny(missing_docs)] use super::detect_chemistry::DetectedProbeBarcodePairingFile; use anyhow::Result; use cr_types::CrMultiGraph; use martian::pre...
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Rust
1,883
51
//! CreateClonotypeClustermap stage code #![expect(missing_docs)] use anyhow::Result; use cr_websummary::ChartWithHelp; use cr_websummary::multi::antigen::{AntigenSpecificityRow, clonotype_specificity_heatmap}; use martian::prelude::*; use martian_derive::{MartianStruct, make_mro}; use martian_filetypes::FileTypeWrite...
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Rust
1,907
63
//! kmer_lookup // Copyright (c) 2018 10X Genomics, Inc. All rights reserved. #![deny(missing_docs)] // Kmer lookup. use debruijn::dna_string::DnaString; use debruijn::kmer::{Kmer12, Kmer20}; use debruijn::{Kmer, Mer, Vmer}; use vector_utils::{lower_bound1_3, upper_bound1_3}; /// Given a vector of DnaStrings dv, cre...
1c6756b19aadbcf6d726d0a9278c4a98c6da191558097c20f5eada1a9a063a58
Rust
1,923
71
//! multi::cmo_set #![deny(missing_docs)] use anyhow::{Result, bail}; use cr_types::GenomeName; use cr_types::reference::feature_reference::{FeatureDef, FeatureType}; use cr_types::types::FeatureBarcodeType; use fastq_set::read_pair::WhichRead; use serde::{Deserialize, Serialize}; use std::collections::HashMap; use st...
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Rust
1,950
55
#![expect(missing_docs)] use anyhow::{Result, bail}; use clap::{ArgAction, Parser}; #[derive(Parser, Debug, Clone, PartialEq, Eq)] pub struct CreateBam { /// Enable or disable BAM file generation. /// Setting --create-bam=false reduces the total computation time and the size of /// the output directory (BA...
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Rust
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66
//! cr_aggr #![deny(missing_docs)] use anyhow::Result; use docopt::Docopt; use martian::prelude::*; use serde::Deserialize; const HEADER: &str = "# Copyright 2023 10x Genomics, Inc. All rights reserved."; const USAGE: &str = " Test Rust stage Usage: cr_aggr martian <adapter>... cr_aggr mro [--file=<filename>] [-...
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Rust
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73
// Copyright (c) 2021 10X Genomics, Inc. All rights reserved. #![deny(missing_docs)] // This file provides the tail end code for join.rs, plus a small function used there. use crate::core::defs::CloneInfo; use equiv::EquivRel; use vector_utils::next_diff1_2; pub(crate) struct JoinResult { pub(crate) i: usize, ...
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Rust
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72
//! This captures and analyzes data about vdj umis. #![deny(missing_docs)] use barcode::Barcode; use debruijn::dna_string::DnaString; use serde::{Deserialize, Serialize}; use umi::Umi; use vdj_types::VdjChain; /// Strongly-typed foreign key index into a vector of contigs #[derive(Clone, Debug, Serialize, Deserialize)...
001cf2071f73037f428b5a00b850e7dbcff609fc5a4de153543927d27a205caf
Rust
2,046
65
#![expect(missing_docs)] use anyhow::Result; use martian::prelude::*; use martian_derive::{MartianStruct, make_mro}; use serde::{Deserialize, Serialize}; use vdj_filter_barcodes::filter_log::FilterSwitch; #[derive(Debug, Clone, Serialize, Deserialize, MartianStruct)] pub struct StageInputs { pub disable_count: Opt...
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Rust
2,054
63
// Copyright (c) 2018 10x Genomics, Inc. All rights reserved. //! Tools for working with groups FASTQ of files. //! Major functionality includes: //! * Find groups FASTQs (R1/R2/I1/I2) following Illumina filename conventions //! * Parsing flowcell information from Illumina FASTQ headers //! * High-speed FASTQ I/O (via ...
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Rust
2,058
60
//! Alarms in the websummary (aka alerts) #![expect(missing_docs)] use cr_types::LibraryType; use serde::{Deserialize, Serialize}; use std::collections::HashSet; #[derive(Debug, Clone, Serialize, Deserialize, PartialEq, Eq, PartialOrd, Ord)] #[serde(rename_all = "UPPERCASE")] pub enum AlertLevel { Error, Warn...
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Rust
2,062
49
#![expect(missing_docs)] use crate::reference::reference_info::ReferenceInfo; use crate::types::GenomeName; use anyhow::Result; use std::path::Path; /// Compute the genome for each chromosome. The output is a vector of genome names in the same /// order as the names of chromosomes in `star/chrName.txt`. If we have mor...
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Rust
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67
#![deny(missing_docs)] use anyhow::Result; use martian::{MartianFileType, MartianRover}; use std::path::Path; /// Attempts to create a hard link a file and creates a copy if hard linking fails /// /// # Arguments /// /// * `src` - The source file path /// * `dst` - The destination link path pub fn hard_link_file<P: As...
b2db7f93dacb429c7375c4f830567455aadb5bba70a0e6d1c20f7e33fca31b7e
Rust
2,155
66
// Copyright (c) 2021 10x Genomics, Inc. All rights reserved. #![deny(missing_docs)] // Populate features. use amino::nucleotide_to_aminoacid_sequence; use vdj_ann::refx::RefData; use vdj_ann::vdj_features::{cdr1_start, cdr2_start, fr1_start, fr2_start, fr3_start}; #[allow(clippy::too_many_arguments)] pub(crate) fn ...
36611b1bce3d4e00e7187d38a465c092935961375602a50cb57eb509ccbe3712
Rust
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61
#![deny(missing_docs)] use crate::barcode_counter::FilteredBarcodeFilter; use cr_h5::molecule_info::MoleculeInfoIterator; use cr_types::TargetingMethod; use cr_types::probe_set::ProbeSetReference; use cr_types::target_panel_summary::{TargetPanelSummary, TargetPanelSummaryFormat}; use cr_types::types::PROBE_IDX_SENTINEL...
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Rust
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69
#![deny(missing_docs)] use crate::websummary::{HeroMetric, PrettyMetric, Threshold}; use serde::{Deserialize, Serialize}; const NUMBER_OF_CELLS: &str = "Total Number of Cells"; const NUMBER_OF_CLONOTYPES: &str = "Total Number of Clonotypes"; const NUMBER_OF_PAIRED_CELLS: &str = "Number of Cells With Productive V-J Sp...
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Rust
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78
#![expect(missing_docs)] use anyhow::Result; use cr_types::chemistry::ChemistryDefs; use cr_types::rna_read::RnaRead; use itertools::Itertools; use martian_derive::{MartianStruct, martian_filetype}; use martian_filetypes::bin_file::BincodeFile; use martian_filetypes::json_file::JsonFile; use martian_filetypes::lz4_file...
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Rust
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72
//! Martian stage WRITE_BARCODE_INDEX //! Assign a distinct integer to each barcode sequence. #![deny(missing_docs)] use anyhow::Result; use cr_types::{ BarcodeIndexFormat, BarcodeIndexOutput, MetricsFile, PerLibrarySortedBarcodeCounts, }; use itertools::Itertools; use json_report_derive::JsonReport; use martian::...
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Rust
2,401
74
use super::logging::Logger; use ndarray::Array2; type FilterTuple = (Array2<f64>, Vec<String>, Vec<String>); pub fn filter_low_counts( norm_matrix: &Array2<f64>, sgrna_names: &[String], gene_names: &[String], min_base: f64, logger: &Logger, ) -> FilterTuple { logger.filtering(min_base); let...
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Rust
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74
//! Martian filetype for representing metrics JSON files. //! Use this type for any metrics JSON file whose contents is not represented //! by a static type. #![expect(missing_docs)] use anyhow::{Context, Result}; use martian::MartianRover; use martian_derive::martian_filetype; use martian_filetypes::FileTypeRead; use ...
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Rust
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63
#![expect(missing_docs)] use bitflags::bitflags; pub const READ_GROUP_TAG: &[u8] = b"RG"; pub const PROC_BC_SEQ_TAG: &[u8] = b"CB"; pub const SPATIAL_BC_TAG: &[u8] = b"sb"; pub const PROC_UMI_SEQ_TAG: &[u8] = b"UB"; pub const FEATURE_RAW_TAG: &[u8] = b"fr"; pub const FEATURE_QUAL_TAG: &[u8] = b"fq"; pub const FEATURE_...
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Rust
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79
//! //! This module implements `Metric` and `JsonReport` for `Option<T>` //! where T is a `Metric` or `JsonReport` //! #![deny(missing_docs)] use crate::{JsonReport, JsonReporter, Metric}; /// `JsonReport` for `Option<J>` where `J: JsonReport` is straightforward. /// We will report J if is it Some and not report it i...
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Rust
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82
use hashbrown::HashMap; use ndarray::{Array1, Axis}; use std::hash::Hash; /// recovers the indices of all unique values in a vector and returns a hashmap of the unique values and their indices /// # Arguments /// * `vec` - the vector to be searched and hashed /// ``` pub fn unique_indices<T: Eq + Hash + Clone>(vec: &[...
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Rust
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84
#![expect(missing_docs)] use crate::transcriptome::Transcriptome; use anyhow::Result; use martian_filetypes::LazyFileTypeIO; use martian_filetypes::tabular_file::TsvFile; use serde::{Deserialize, Serialize}; use std::collections::HashMap; #[derive(Clone, Debug, PartialEq, Eq)] pub struct TranscriptIndex { pub tran...
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Rust
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73
//! Martian stage GET_GDNA_METRICS #![deny(missing_docs)] use crate::gdna_utils::compute_gdna_metrics; use anyhow::Result; use cr_types::H5File; use cr_types::reference::probe_set_reference::TargetSetFile; use martian::{MartianMain, MartianRover}; use martian_derive::{MartianStruct, make_mro}; use martian_filetypes::F...
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Rust
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69
//! multiconfig_converter #![deny(missing_docs)] use anyhow::Result; use multi::config::MultiConfigCsv; use serde_json::to_vec; use std::slice::{from_raw_parts, from_raw_parts_mut}; fn safe_convert_multi_csv(input: &[u8]) -> Result<Vec<u8>> { let config = MultiConfigCsv::from_reader(input, "api")?; Ok(to_vec(&...
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Rust
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60
#![expect(missing_docs)] use crate::env::PkgEnv; use crate::utils::AllArgs; use anyhow::{Result, bail}; use serde_json; use std::env; use std::fs::read_to_string; use std::path::Path; use std::process::ExitCode; use url::Url; pub fn run_cloud(pkg_env: &PkgEnv, args: &AllArgs) -> Result<ExitCode> { // target the te...
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Rust
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//! Martian stage WRITE_MINIMAP_INDEX #![deny(missing_docs)] use anyhow::{Result, anyhow, bail}; use cr_types::{AlignerParam, MinimapPreset}; use martian::prelude::*; use martian_derive::{MartianStruct, make_mro, martian_filetype}; use martian_filetypes::{LazyFileTypeIO, LazyWrite}; use minimap2::{Aligner, Preset}; us...
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Rust
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73
//! HandleNoClonotyping stage code #![expect(missing_docs)] use anyhow::{Context, Result}; use martian::prelude::*; use martian_derive::{MartianStruct, make_mro}; use martian_filetypes::json_file::JsonFile; use serde::{Deserialize, Serialize}; use vdj_ann::annotate::ContigAnnotation; #[derive(Debug, Clone, Deserializ...
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Rust
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94
//! cr_lib #![deny(missing_docs)] mod align_and_count_metrics; mod align_metrics; pub(crate) mod io; /// Align homopolymer sequences. pub mod align_homopolymer; /// Align reads, annotate, deduplicate UMI and count genes for /// one group of reads from a single barcode mod aligner; /// Barcode correction stage (and ...
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Rust
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use anyhow::{bail, Result}; use ndarray::{Array1, Array2, ArrayView1, Axis}; use ndarray_stats::SummaryStatisticsExt; use std::ops::{Div, Mul}; /// Calculates the median of a provided ndarray pub fn median(array: &ArrayView1<f64>) -> f64 { let mut sorted = array.to_vec(); sorted.sort_by(|a, b| a.partial_cmp(b)...
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Rust
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71
//! Martian stage COPY_CHEMISTRY_SPEC //! This is a shim stage to adapt older pipelines that only accept a single //! chemistry spec from the user into the spec-per-library-type required by //! DETECT_CHEMISTRY. //! //! This stage can also handle expanding a ChemistrySet into the individual //! chemistries required for...
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Rust
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81
//! Detect accidental duplication of FASTQ files. #![deny(missing_docs)] use super::chemistry_filter::DetectChemistryUnit; use anyhow::{Result, bail}; use fastq_set::read_pair::{ReadPair, ReadPart, WhichRead}; use metric::{TxHashMap, TxHasher}; use std::collections::hash_map::Entry; use std::hash::Hasher; use std::pat...
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Rust
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95
//! Barcodes which are defined based segmented spatial cells #![expect(missing_docs)] use anyhow::ensure; use pyo3::{pyclass, pymethods}; use serde::{Deserialize, Serialize}; use std::fmt::{Display, Formatter}; use std::str::FromStr; pub(super) const CELL_ID_PREFIX: &str = "cellid"; #[derive(Serialize, Deserialize, C...
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Rust
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//! Martian stage COMBINE_CHEMISTRY_DEFS #![deny(missing_docs)] use anyhow::{Result, ensure}; use cr_types::MetricsFile; use cr_types::chemistry::{ChemistryDef, ChemistryDefs, ChemistryDefsExt}; use cr_types::types::{LibraryType, VdjChainType}; use itertools::{Itertools, zip_eq}; use martian::{MartianMain, MartianRove...
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Rust
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93
#![deny(missing_docs)] use itertools::Itertools; use metric::TxHashMap; use pyanyhow::Result; use pyo3::prelude::*; use pyo3::types::{PyBytes, PyDict}; use serde::{Deserialize, Serialize}; #[pyclass(module = "cellranger.fast_utils")] #[derive(Clone, Serialize, Deserialize)] pub struct MatrixBarcodeIndex(TxHashMap<Str...
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Rust
2,754
81
//! Module for handling bazel specific quirks such as path resolution #![deny(missing_docs)] use anyhow::{Result, bail}; use std::env; use std::path::{Path, PathBuf}; /// Canonicalize a path pub fn canonicalize(path: &Path) -> Result<PathBuf> { use std::path::Component::CurDir; let path = if path.is_absolute(...
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Rust
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81
//! Martian stage WRITE_H5_MATRIX. #![deny(missing_docs)] use crate::utils::estimate_mem::barcode_mem_gib; use anyhow::Result; use cr_h5::count_matrix::write_matrix_h5; use cr_types::chemistry::{ChemistryDefs, ChemistryDefsExt}; use cr_types::{BarcodeIndexOutput, CountShardFile, FeatureReferenceFormat, H5File}; use ma...
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Rust
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93
//! Secondary analysis pipeline for testing #![deny(missing_docs)] use crate::stage_testing::run_stage; use crate::stages::pca::{PcaStage, PcaStageInputs}; use crate::stages::umap::{UmapStage, UmapStageInputs}; use crate::types::{H5File, ReductionType}; use anyhow::Result; use martian::prelude::*; use martian_derive::...
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Rust
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72
// Copyright (c) 2018 10X Genomics, Inc. All rights reserved. //! This file contains some miscellaneous string utilities. #![deny(missing_docs)] // ▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓ // THINGS USED A LOT: SHORTHAND EXPRESSIONS FOR COMMON FUNCTIONALITY // ▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓...
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Rust
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79
//! Standard chemistry definitions imported from a json file #![deny(missing_docs)] use crate::chemistry::{ChemistryDef, ChemistryName}; use anyhow::{Context, Result}; use itertools::Itertools; use lazy_static::lazy_static; use std::collections::HashMap; const CHEMISTRY_DEFS_JSON_STR: &str = std::include_str!("chemis...
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Rust
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82
#![expect(missing_docs)] use crate::arc::count::CountMro; use crate::env; use crate::fastqs::FastqArgs; use crate::mrp_args::MrpArgs; use crate::utils::{CliPath, validate_id}; use anyhow::Result; use clap::{self, Parser}; use cr_types::LibraryType; use ordered_float::NotNan; use std::path::PathBuf; /// Executes the co...
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Rust
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75
#![expect(missing_docs)] use anyhow::{Result, bail, ensure}; use clap::{self, Parser}; use ordered_float::NotNan; use serde::Serialize; use std::collections::HashMap; #[derive(Parser, Debug, Clone)] pub struct ForceCellsArgs { /// Cell caller override: define the minimum number of ATAC transposition events ///...
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Rust
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//! cr_vdj #![deny(missing_docs)] use anyhow::Result; use docopt::Docopt; use martian::prelude::*; use serde::Deserialize; const HEADER: &str = "# Copyright 2023 10x Genomics, Inc. All rights reserved."; const USAGE: &str = " Test Rust stage Usage: cr_vdj martian <adapter>... cr_vdj mro [--file=<filename>] [--re...
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Rust
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72
//! barcode_extensions #![deny(missing_docs)] use barcode::corrector::Posterior; use barcode::{ BarcodeConstruct, BarcodeCorrector, BcSegSeq, CorrectSubNotIndel, Whitelist, WhitelistSpec, }; use cr_types::chemistry::BarcodeExtraction; use metric::SimpleHistogram; use std::ops::Range; #[cfg(feature = "tenx_interna...
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Rust
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90
#![deny(missing_docs)] use barcode::whitelist::ReqStrand; use minimap2::ffi::{MM_F_OUT_MD, MM_F_SPLICE_FOR, MM_F_SPLICE_REV}; use minimap2::{Aligner, Built, Preset}; use rust_htslib::bam::{Header, HeaderView, Record}; use std::path::Path; use std::sync::Arc; pub struct MinimapReference { pub aligner: MinimapAlign...
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Rust
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101
//! Measure fraction of local wall time consumed by an action. use std::io::{Write, stderr}; use std::time::{Duration, Instant}; /// Quick one-liner function to time a single action. /// /// Writes the elapsed time to stderr. pub fn timed<T>(name: &str, action: impl FnOnce() -> T) -> T { let start = Instant::now(...
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Rust
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//! //! ALign reads to a contig #![deny(missing_docs)] use bio::alignment::Alignment; use bio::alignment::pairwise::{self, MatchParams, banded}; use bio::alignment::sparse::{HashMapFx, find_kmer_matches_seq2_hashed, hash_kmers}; pub type Scoring = pairwise::Scoring<MatchParams>; pub struct ContigAligner<'a> { con...
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Rust
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//! websummary_derive #![deny(missing_docs)] use proc_macro::TokenStream; use quote::quote; use syn::{Fields, ItemStruct}; const ALERT_NOT_ON_NAMED_STRUCT_ERROR: &str = r"#[derive(Alert)] can only be used on structs with named fields."; /// Implement #[derive(Alert)] macro #[proc_macro_derive(Alert)] pub fn webs...
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Rust
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#![deny(missing_docs)] use cr_types::rna_read::RnaRead; use fastq_set::WhichEnd::{FivePrime, ThreePrime}; use fastq_set::adapter_trimmer::{Adapter, AdapterLoc, ReadAdapterCatalog}; use fastq_set::adapters::{ ILLUMINA_P5_RC, ILLUMINA_P7_RC, ILLUMINA_R1_RC, ILLUMINA_R2_RC, POLY_A, POLY_T, RT_PRIMER, RT_PRIMER_RC,...
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Rust
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use super::inverse; use ndarray::{Array1, Array2, Axis}; /// An implementation of [Weighted Least Squares](https://en.wikipedia.org/wiki/Weighted_least_squares) using a Matrix/Vector Formulation #[derive(Debug)] pub struct Wols { alpha: f64, beta: f64, } impl Wols { /// Fits a Weighted Least Squares Linear...
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Rust
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99
use hashbrown::HashSet; /// Sets the number of threads globally pub fn set_threads(threads: usize) { rayon::ThreadPoolBuilder::new() .num_threads(threads) .build_global() .unwrap(); } /// Generates default sample names pub fn generate_sample_names(input_paths: &[String]) -> Vec<String> { ...
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Rust
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108
use crate::{ aggregation::GeneAggregation, enrich::TestStrategy, model::ModelChoice, norm::Normalization, }; use adjustp::Procedure; use bon::{builder, Builder}; use getset::Getters; #[derive(Debug, Getters, Builder)] #[getset(get = "pub")] pub struct Configuration<'a> { #[builder(default)] normalization: ...
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Rust
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118
mod compute_aggregation; mod results; mod utils; use clap::ValueEnum; pub use compute_aggregation::compute_aggregation; pub use results::AggregationResult; /// Enum describing aggregation procedure selection #[derive(ValueEnum, Clone, Debug, PartialEq)] #[allow(clippy::upper_case_acronyms)] pub enum GeneAggregationSe...
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Rust
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93
use crate::{ aggregation::compute_aggregation, enrich::enrichment_testing, io::{GeneFrame, HitList, Screenviz, SgrnaFrame, SimpleFrame}, model::model_mean_variance, norm::normalize_counts, utils::{config::Configuration, filter::filter_low_counts, logging::Logger}, }; use anyhow::Result; use rege...
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Rust
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106
use super::inverse; use ndarray::{Array1, Array2, Axis}; /// An implementation of [Ordinary Least Squares](https://en.wikipedia.org/wiki/Ordinary_least_squares#Matrix/vector_formulation) using a Matrix/Vector Formulation #[derive(Debug)] pub struct Ols { alpha: f64, beta: f64, } impl Ols { /// Fits an Ordi...
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Rust
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100
#![deny(missing_docs)] use rand::rngs::SmallRng; use rand::{RngExt, SeedableRng}; use std::iter::IntoIterator; /// Randomly choose a sample of k items from a list containing n items, /// where n is often a very large or unknown number /// See https://en.wikipedia.org/wiki/Reservoir_sampling pub struct ReservoirSampler...
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Rust
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106
#![deny(missing_docs)] use anyhow::Result; use hdf5::Group; use hdf5::types::FixedAscii; use itertools::Itertools; use serde::{Deserialize, Serialize}; use std::collections::HashMap; use std::path::Path; martian_derive::martian_filetype! {H5File, "h5"} pub fn open_matrix_group(matrix_path: &Path) -> Result<Group> { ...
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Rust
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100
#![expect(missing_docs)] #[allow(clippy::enum_glob_use)] use AutoChemistryName::*; #[allow(clippy::enum_glob_use)] use AutoOrRefinedChemistry::*; #[allow(clippy::enum_glob_use)] use ChemistryName::*; #[allow(clippy::enum_glob_use)] use ChemistryParam::*; #[allow(clippy::enum_glob_use)] use ChemistrySet::*; use anyhow::...
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Rust
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//! Martian stage SUMMARIZE_MATRIX_DIMS //! Summarize the dimensions of an H5 matrix. #![deny(missing_docs)] use anyhow::Result; use cr_h5::count_matrix::CountMatrixFile; use cr_types::MetricsFile; use cr_types::probe_set::ProbeType; use cr_types::reference::feature_reference::FeatureType; use cr_types::reference::pro...
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Rust
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81
//! FilterExactClonotypes stage code #![expect(missing_docs)] use anyhow::Result; use martian::prelude::*; use martian_derive::{MartianStruct, make_mro}; use martian_filetypes::json_file::JsonFile; use martian_filetypes::{LazyFileTypeIO, LazyWrite}; use serde::{Deserialize, Serialize}; use std::collections::HashSet; u...
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Rust
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118
//! amino // Copyright (c) 2019 10x Genomics, Inc. All rights reserved. #![deny(missing_docs)] use debruijn::Mer; use debruijn::dna_string::DnaString; /// Test to see if a given DnaString has a start or stop codon at a given position. pub fn have_start(b: &DnaString, j: usize) -> bool { let (a, g, t) = (0u8, 2u8,...
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Rust
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102
use crate::{ aggregation::compute_aggregation, enrich::enrichment_testing, io::{ get_string_column, match_headers_from_regex_set, to_ndarray, validate_ntc, write_gene_frame, write_hit_list, write_sgrna_dataframe, Screenviz, }, model::model_mean_variance, norm::normalize_counts, ...
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Rust
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115
//! cr_types #![deny(missing_docs)] use anyhow::{Context, Result, ensure}; use csv_parser::CsvParser; use std::fs::{copy, hard_link}; use std::path::{Path, PathBuf}; pub mod action_timer; pub mod aggr; pub mod barcode_index; pub mod cell_annotation; pub mod chemistry; pub mod clonotype; pub mod constants; pub mod csv...
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Rust
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106
use std::time::Duration; use indicatif::{MultiProgress, ProgressBar, ProgressStyle}; /// Initializes a Multiprogress bar for parallel logging #[must_use] pub fn initialize_multi_progress( sample_names: &[String], ) -> (Option<MultiProgress>, Option<Vec<ProgressBar>>) { let mp = MultiProgress::new(); let p...
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Rust
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//! enclone_process // Copyright (c) 2021 10x Genomics, Inc. All rights reserved. #![deny(missing_docs)] mod core; mod disintegrate; mod doublets; mod enclone; mod filter_umi; mod flag_defective; mod merge_onesies; mod populate_features; mod process; mod read_json; mod some_filters; mod split_candidate_clonotypes; mod...
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Rust
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103
use anyhow::Result; use ndarray::{Array1, Array2, Axis}; use rand::Rng; use rand_distr::{Binomial, Distribution}; use std::ops::{Div, Sub}; /// Z-Score Transforms the Provided Array /// # Arguments /// * `array` - the array to be transformed pub fn zscore_transform(array: &Array1<f64>) -> Array1<f64> { let mu = ar...
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Rust
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#![expect(missing_docs)] use serde::{Deserialize, Serialize}; /// Different reasons why a barcode which have productive contig(s) /// are not called as cells by enclone /// /// For more explanation, see <https://10xgenomics.github.io/enclone/pages/auto/default_filters.html> /// and <https://10xgenomics.github.io/enclo...
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Rust
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105
//! Align homopolymer sequences. #![deny(missing_docs)] use std::cmp::max; use std::ops::Range; /// Construct the score vector. fn construct_score_vector(nt: u8, seq: &[u8]) -> Vec<i64> { const MATCH_SCORE: i64 = 1; const MISMATCH_SCORE: i64 = -9; let scoring_function = |x| { if x == nt { MATCH_SC...
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Rust
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mod compute_aggregation; mod results; mod utils; use clap::ValueEnum; pub use compute_aggregation::compute_aggregation; use geopagg::WeightConfig; pub use results::AggregationResult; /// Enum describing aggregation procedure selection #[derive(ValueEnum, Clone, Debug, PartialEq)] #[allow(clippy::upper_case_acronyms)]...
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Rust
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//! Martian stage GENERATE_CAS_METRICS //! Generate cell annotation metrics json #![deny(missing_docs)] use crate::cell_annotation_ws_parameters::{CellAnnotationMetrics, CellTypeInfo, MetadataStruct}; use anyhow::{Ok, Result}; use martian::prelude::*; use martian_derive::{MartianStruct, make_mro}; use martian_filetypes...
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Rust
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109
use crate::aggregation::AggregationResult; use anyhow::Result; use ndarray::Array1; use std::{ fs::File, io::{BufWriter, Write}, }; pub struct GeneFrame<'a> { gene: &'a [String], gene_fc: &'a Array1<f64>, gene_log2_fc: &'a Array1<f64>, score_low: &'a Array1<f64>, pvalue_low: &'a Array1<f64>...
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Rust
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106
use crate::enrich::EnrichmentResult; use anyhow::Result; use ndarray::Array1; use std::{ fs::File, io::{BufWriter, Write}, }; pub struct SgrnaFrame<'a> { sgrna_names: &'a [String], gene_names: &'a [String], adj_var: &'a Array1<f64>, base: &'a Array1<f64>, control: &'a Array1<f64>, treat...
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Rust
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105
// Copyright (c) 2021 10X Genomics, Inc. All rights reserved. #![deny(missing_docs)] // // If NWEAK_ONESIES is not specified, disintegrate certain onesie clonotypes into single cell // clonotypes. This requires editing of exact_clonotypes, info, eq, join_info and raw_joins. use crate::core::defs::{CloneInfo, ExactClo...
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Rust
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//! AsmMetrics stage code #![expect(missing_docs)] use crate::assembly::{BarcodeDataFile, VdjPrimers}; use anyhow::{Context, Result}; use cr_types::MetricsFile; use cr_types::chemistry::ChemistryDef; use martian::prelude::*; use martian_derive::{MartianStruct, make_mro, martian_filetype}; use martian_filetypes::json_f...
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Rust
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106
// Copyright (c) 2018 10x Genomics, Inc. All rights reserved. #![expect(missing_docs)] //! Sized, stack-allocated container for a short quality string. use crate::array::{ArrayContent, ByteArray}; use crate::metric_utils::ILLUMINA_QUAL_OFFSET; use std::iter::Iterator; use std::str; #[derive(Clone, Copy, PartialOrd, ...
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Rust
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#![expect(missing_docs)] use anyhow::{Context, Result}; const CLONOTYPE_PREFIX: &str = "clonotype"; const CONSENSUS_PREFIX: &str = "consensus"; const CONCAT_REF: &str = "concat_ref"; #[derive(Debug, Clone, PartialEq, Eq, PartialOrd, Ord)] pub struct ClonotypeId<'a> { pub id: usize, pub sample_id: Option<&'a s...
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Rust
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146
// TODO: fix these. #![expect(missing_docs)] #![allow(clippy::needless_range_loop)] use lz4; use rust_htslib::bam::Record; use rust_htslib::bam::record::Aux; use std::fs::File; use std::io::{BufWriter, Write}; use string_utils::TextUtils; pub fn write_sam_record_simple( x: &Record, u: &str, // corrected umi ...
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Rust
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use cr_h5::count_matrix::{ BarcodeCountOffset, Count, CountMatrixFile, CountMatrixStreaming, FeatureIdx, MatrixDimensions, }; use cr_types::reference::feature_reference::FeatureType; use numpy::{IntoPyArray, PyArray1}; use pyo3::exceptions::PyTypeError; use pyo3::prelude::*; use std::iter; use std::path::Path; use ...
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Rust
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// Copyright (c) 10x Genomics, Inc. All rights reserved. //! Iterator adapter for post-hoc trimming of read pair lengths. //! //! This is the preferred mechanism for applying Illumina read-length trimming //! because it operates as a final step after all read-source adapters have been //! composed (e.g. the long-read ...
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Rust
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113
use anyhow::Result; use polars::prelude::*; use std::{fs::File, io::BufWriter}; use crate::{ aggregation::{AggregationResult, GeneAggregation}, utils::{config::Configuration, logging::Logger}, }; fn build_gene_frame(results: &AggregationResult) -> Result<DataFrame, PolarsError> { df!( "gene" => re...
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Rust
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122
#![deny(missing_docs)] use crate::transcriptome::Exon; use anyhow::Result; use bio::io::fasta::IndexedReader; use bio_types::strand::ReqStrand; use itertools::Itertools; use std::io::{Read, Seek}; /// Construct a transcript sequence from a FASTA file, a strand, and a set of intervals /// The intervals must be ordered ...
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Rust
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100
//! vdj_ann_ref // Copyright (c) 2018 10X Genomics, Inc. All rights reserved. #![deny(missing_docs)] // This file contains code to make reference data. // // ◼ Experiment with building a reference from scratch. This would be a better // ◼ solution than ad hoc editing of a flawed reference. // // ◼ Document reference ...
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Rust
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//! Martian stage RUN_PCA2 //! Replacement for RUN_FBPCA. Could be absorbed into RUN_PCA_NG stage after some refactoring #![expect(missing_docs)] use crate::io::h5; use crate::types::H5File; use anyhow::Result; use hdf5_io::matrix::read_adaptive_csr_matrix; use martian::MartianVoid; use martian::prelude::*; use martia...
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Rust
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101
//! Martian stage SETUP_VDJ_DEMUX #![deny(missing_docs)] use crate::{GexMatrices, SampleMatrices}; use anyhow::{Context, Result}; use cr_types::{BarcodeMultiplexingType, CrMultiGraph, FingerprintFile}; use martian::prelude::*; use martian_derive::{MartianStruct, make_mro}; use martian_filetypes::json_file::JsonFile; u...