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81e39a7a184d085a657e0c323fbcb1505dae7ea0d670e29f5940be76b3928844
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fluidPage( fluidRow( column( width = 6, style = "padding:0px;" ), column( width = 6, uiOutput("IwateValueBoxes") %>% withSpinner(proxy.height = "200px") ) ), fluidRow( box( width = 12, closable = F, title = tagList(icon("chart-line"), sprintf(i18n$t("%sの発生状況...
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--- title: "Length of stay in the ICU" author: "tom pollard" description: "Length of stay in the ICU for patients in MIMIC-III" output: pdf_document date: "10/10/2017" --- ```{r setup, include = FALSE} knitr::opts_chunk$set(echo = TRUE) # install.packages("ggplot2") # install.packages("bigrquery") library("ggplot2")...
1d117390b53aaa11411d3c1b6584b09dc0deca7f598f8f9e891496b5a907acfc
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#first script to launch before building your network with metacore #Code to preprocess data so that they are usable for metacore and also the Network pipeline #From the DEGs in all cell types you are getting a file which is ending woth "List.txt" and can be directly copy paste into metacore #You are also getting the ...
485154986ce39210c4e86b96eeeeabdc737d3b2dbc312e45c2c29987053e9ea3
R
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library("tidyverse") library("here") library("sessioninfo") halo_files <- list.files(here("raw-data", "HALO"), full.names = TRUE) names(halo_files) <- gsub(".csv", "", basename(halo_files)) halo_anno <- map2(halo_files, names(halo_files), function(fn, samp) { output <- read_csv(fn) %>% mutate( ...
3291511db72718defe4a8686bfd72e360d07fbc206cf56a75bf517587f4286ac
R
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# 20231125 ֮ǰʹÓõÄsnpEFF¶ÔUCRÉÏËùÓеÄSNP½øÐÐÁË×¢ÊÍ£¬ÏÖÔÚɸѡ³öHIGHµÄSNP£¬ÓÃÓÚÏÂÒ»²½ÔÚVEPµ±ÖÐÔ¤²âÄÄЩÓë¼²²¡Ïà¹Ø rm(list = ls()) setwd(dir = "D:/R_project/UCR_project") library(tidyverse) UCRSNPAnno <- read.table(file = "D:/A_projects/UCR/snpEFFAnno/UCRSNPAnno.vcf", sep = "\t", skip = 7) c...
98c7a2d20b25e4de06f2abf93ac8aa54549ca65f2f944bd9bf8fdd0cafafe672
R
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## 02_harmony_integration.R library(Seurat) library(harmony) library(scCustomize) set.seed(1234) # Process Li2023 dataset li_obj <- readRDS("li2023_merged.RDS") li_obj <- NormalizeData(li_obj) li_obj <- FindVariableFeatures(li_obj, nfeatures = 3000) li_obj <- CellCycleScoring(li_obj, s.features = cc.genes$s.genes, g2...
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R
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options(max.print = 1000) options(stringsAsFactors = FALSE) options(scipen = 999) library(data.table) args = commandArgs(trailingOnly=TRUE) # CpG positions ## CpG annotations # load a <- fread(args[1], select = c("V1", "V2", "V4")) names(a) <- c("chrom", "ref_position", "probe_id") # a # collapse by strand a2 <- ...
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R
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options(stringsAsFactors = FALSE) library(ggplot2) library(reshape2) library(dplyr) library(stringr) library(lme4) library(lmerTest) library(RColorBrewer) library(ggpubr) library(parallel) library(MutationalPatterns) ref_genome="BSgenome.Hsapiens.UCSC.hg19" chr_orders=c(paste("chr",1:22,sep=""),"chrX","chrY","chrM") li...
c2f74d968caeb2c0b1f69a8554d08160c07757f2e8c96feafc905ce9fe859cb3
R
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library(RColorBrewer) library(pheatmap) currPath <- dirname(rstudioapi::getSourceEditorContext()$path) setwd(currPath) setwd('..') setwd('..') setwd("Modeling_CRC/Plots") ############ Japanese diet ######################## #### Species correlations ##### taxonomy=read.csv("Taxonomy_JD_Species.csv",head...
6499b173bd7a52a8d0952900b0eb8b8767373e91de7e6c6da27b2d8c5ff91ecf
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region.members <- function(intervals, positions) { stopifnot(is.data.frame(intervals)) stopifnot(all(c("chr","start","end") %in% colnames(intervals))) stopifnot(is.data.frame(positions)) stopifnot(all(c("chr","pos") %in% colnames(positions))) events <- rbind(data.frame(chr=as.character(interval...
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#!/usr/bin/env R # # get the sce comprising just identified marker genes # # Notes: # # * ran on jhpce using the following, with "NMEM" as either 10G or 100G: # # request more mem with qrsh # qrsh -l mem_free=100G,h_vmem=100G # module load conda_R/4.1 # R # # # Troubleshooting: # # * PROBLEM: when using as.matr...
0764c82a5e70b2997e5f7ef9548fe9852d8685700f69fea4346e9885307a03ac
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################################################################################ ### LIBD pilot 10x-Frankenstein (n=12) snRNA-seq samples ### ** PAN-BRAIN ANALYSIS ** ### - R-batch job for detxn of optimal PC space with ~'sce.pilot.n12'~ object ### MNT 07-08Feb2020 (now on 'sce.all.n12') ~29-30Jan202...
593abf0eb53b6a43ada5e6b58116f13f1c4ea9283f22313409ac7ce1dafef426
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# Siwei 29 Feb 2024 # Make line-dot and circus plots for Alena's PICALM paper # init #### library(readxl) library(ggplot2) library(RColorBrewer) ## make line-dot plot ##### df_raw <- read_excel("IPA_pathway_for_graphing.xlsx", sheet = 2) df_2_plot <- df_raw df_2_plot$Ingenuity_Canonical_Pathways <- ...
cc61260c8f78f91b0327431ba8f28d8b460343eed6a31ce24f1af02e3fd455d9
R
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output$renderCalendar <- renderUI({ if (input$linePlot == "confirmed") { if (is.null(input$regionPicker)) { tags$p("未選択です。地域を選択してください。") } else { tagList( echarts4rOutput("confirmedCalendar", height = "130px"), calendarDateRangePicker("confirmCalendarDateRange") ) } } e...
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# ============================================================================= # Seurat标准流程脚本 # ============================================================================= # 功能:合并正常与疾病样本,进行Seurat标准流程分析 print("Hello world!") rm(list = ls()) # 加载配置文件 source("../config.R") # 设置工作目录 setwd(ENV_DIR) lf <- list.files("....
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library(tidyverse) library(hyprcoloc) total_result <- data.frame() inputpath <- "/mnt/data/lijincheng/mGWAS/result/02MRBMA/hyprcoloc/MVinput_res/" inputfiles <- list.files(inputpath,pattern="_MVinput.RDS$",full.names = T) full_name <- gsub("_MVinput.RDS$", "", basename(inputfiles)) for(i in 1:length(inputfiles)){ ...
cf18ba6d6a0fe7d5649ad35bb73fe0d762be7d3ec5929596d9a8def7dc5694e3
R
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# ============================================================================= # 样本差异表达分析脚本 # ============================================================================= # 功能:按样本进行差异表达分析 print("Hello world!") rm(list = ls()) # 加载配置文件 source("../config.R") # 设置工作目录 setwd(ENV_DIR) lf <- list.files("./") for (file i...
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R
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freeze_features <- c("freeze_time_sec", "freeze_behavior_proportion", "freeze_num_bouts", "freeze_avg_bout_lens", "freeze_first_bout") #"freeze_bout_3sec", "freeze_bout_6sec", "freeze_bout_9sec" circle_features <- c("circle_num_bouts") of_features <- c("f2_distance_cm", "f2_center_distance_cm", "f2_periphery_distance_c...
ab1d5b4d8ade6e42a645e649ed36e7491211fd954d14066ec924108dc0f20e28
R
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fluidRow( Component.MainValueBox( mainValue = sum(mhlwSummary[日付 == max(日付)]$検査人数), mainValueSub = getFileUpdateTime(mhlwSummaryPath), sparklineName = "pcrSparkLine", diffNumber = (sum(mhlwSummary[日付 == max(日付)]$検査人数) - sum(mhlwSummary[日付 == max(日付) - 1]$検査人数, na.rm = T)), text = i18n$t("検査人数"), ...
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R
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data <- read.csv('E:\\path_to_repo\\statsmodels\\datasets\\grunfeld\\grunfeld.csv') #data <- read.csv('../../../statsmodels/datasets/grunfeld/grunfeld.csv') data <- data[data$firm %in% c('General Motors','Chrysler','General Electric','Westinghouse','US Steel'),] attach(data) library('plm') library('systemfit') panel <...
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# exonicµÄgo¸»¼¯·ÖÎö½á¹û setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(ggplot2) library(readxl) library(stringr) library(dbplyr) exonic_UCR <- read_xlsx(path = "01-data/10-GO_enrichment_analysis/exonic_GO_enrichment_analysis.xlsx", sheet = 2) exonic_UCR$...
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R
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suppressPackageStartupMessages(library(optparse)) option_list = list( make_option(c("-m", "--feature_map"), type='character', default = NULL, help="Create up/downstream introns by intron map of circRNA to intron mapping (tsv)"), make_option(c("-i", "--introns"), type='character', hel...
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R
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as_regulon_network = function(regulons){ regulators = regulons[['regulator']] %>% unique() regulons = sapply(regulators, function(regulator_oi){ X = regulons %>% filter(regulator %in% regulator_oi) X = list( tfmode = setNames(X[['tfmode']], X[['target']])...
2a0c455867f51c43f4e4a66e5e14c9a172624ef20a444702f8464b9d298b8b20
R
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#' Similar to the base sweep function, but can be used for sparse matrices as well. #' #' Enables fast computations across columns or rows (margin=1 and 2 respectively) #' Only matrix, dgeMatrix, dgTMatrix, dgCMatrix, or dgRMatrix types are supported. #' adapted from https://stackoverflow.com/questions/55407656/r-sweep...
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#------------------------------------------------------------ Experiment UI ---- experiment_ui = function(id) { ns = shiny::NS(id) bs4Dash::tabsetPanel( type = "tabs", shiny::tabPanel( title = "Metadata", shiny::uiOutput( outputId = ns('up_metadata_ui') ) ), shiny::tabPane...
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suppressPackageStartupMessages(library(optparse)) option_list = list( make_option(c("-g", "--gene_set"), type='character', default = NULL, help="Features for gene set"), make_option(c("-f", "--format"), type = 'character', default = 'tsv', help = 'Format of gene_set. Either \'gff\' or...
2cff8d8305d7c3c9aa2233d13aaaac784bc827c5e385da1b3968994907f5ef62
R
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45
library(introdataviz) library(tidyr) library(dplyr) library(ggplot2) library(ggpubr) exp <- readRDS("~/Desktop/phd/pBrain/data/primateBrain_kznfTEexp.rds") # split gene and TE dataframe kznf_exp <- exp[,c(1:285, 1191, 1192)] kznf_exp_longer <- kznf_exp %>% mutate(sample = rownames(.)) %>% pivot_longer(cols = ...
6b03861ca12e1df787914735c9f91a4dda806af3b3ebbc953bbf7dcd8ffcf17f
R
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library(EnsDb.Hsapiens.v86) ensdb <- EnsDb.Hsapiens.v86::EnsDb.Hsapiens.v86 columns(ensdb) head(keys(ensdb)) head(keytypes(ensdb)) keytypes(ensdb) head(keys(ensdb,keytype = "GENEBIOTYPE")) library(TxDb.Hsapiens.UCSC.hg38.knownGene) txdb <- TxDb.Hsapiens.UCSC.hg38.knownGene::TxDb.Hsapiens.UCSC.hg38.knownGene keytypes...
77ef583f880736d38e88ccbd8b7e8c73aecca14bd2df9e5f757bc61aafaeda74
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library(data.table) source(file = "01_Settings/Path.R", local = T, encoding = "UTF-8") gMobility <- fread(paste0(DATA_PATH, "Google/Global_Mobility_Report.csv")) jpDt <- gMobility[country_region_code == "JP"] nameJa <- list( "Hokkaido" = "北海道", "Aomori" = "青森", "Iwate" = "岩手", "Miyagi" = "宮城", "Akita" = "秋田...
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library("SingleCellExperiment") library("rafalib") library("iSEE") library("lobstr") library("here") library("whisker") library("usethis") library("withr") library("rsconnect") library("sessioninfo") load(here("rdas", "revision", "regionSpecific_HPC-n3_cleaned-combined_SCE_MNT2021.rda"), verbose = TRUE) source(here("...
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shrink.candidates <- function(start.idx, end.idx, FUN, ...) { stopifnot(length(start.idx) == length(end.idx)) stopifnot(all(start.idx <= end.idx)) do.call(rbind, parallel::mclapply(1:length(start.idx), function(i) { shrink.candidate(start.idx[i], end.idx[i], FUN, ...) })) } shrink.candidate...
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R
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arg = commandArgs(trailingOnly=T) lst = read.table(arg[1],as.is=T)[,1] names = gsub(".wgt.RDat","",basename(lst)) N = length(lst) models = c("top1","blup","enet","bslmm","lasso") colnames = c( "id" , "nsnps" , "hsq" , "hsq.se" , "hsq.pv" , paste(models,"r2",sep='.') , paste(models,"pv",sep='.') ) mat.snps = matrix(n...
46e3646cb2670d020af7076c6e43af29f71117942c1fe8884182a0899d9bcdcd
R
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## Plot bulk fetal Age DMRs ## #-------------------------------------------------------------------------------------------------------------------------------- # To fix bug with cache associated with AnnotationHub: # From http://bioconductor.jp/packages/3.13/bioc/vignettes/AnnotationHub/inst/doc/TroubleshootingThe...
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library(tidyverse) library(ggstatsplot) deg <- "/Volumes/T9/drosophila_nanopore/DRS_compairison/results/Excel/DE/tau_ctrl_results-nanocount_with_TPMs.csv" polyA <- "/Volumes/T9/drosophila_nanopore/DRS_compairison/results/Excel/polyA_length/polya_diff_per_transcript.csv" m6a <- "/Volumes/T9/drosophila_nanopore/DRS_com...
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R
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library(tidyverse) # find paths to all kmer distrivution tables for different comparisons dbrn_paths <- list.files(path = "data/peka_papa/2023-12-15_papa_cryptics_kmer6_window_250_distal_window_500_relpos_0", pattern = "_6mer_distribution_genome.tsv$", recursive = T, ...
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tabPanel( title = tagList( "ワクチンの接種", boxLabel("New", status = "warning") ), icon = icon("syringe"), value = "vaccine", fluidRow( column( width = 8, tags$br(), echarts4rOutput("vaccine_line_plot") %>% withSpinner() ), column( width = 4, tags$br(), tags$p...
011822d97b75cab5f3baed5787a1abea342ca1f186a30985325d57cd5f3af3b4
R
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```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) library(WVPlots) library(clue) library(tidyverse) library(dplyr) library(tidyr) library(ggplot2) library(ggExtra) library(cowplot) theme_set(theme_cowplot()) library(corrplot) library(visreg) library(ggcorrplot) library(ggseg3d) library(ggseg) library(ggseg...
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library(STRINGdb) library(org.Hs.eg.db) library(dplyr) library(clusterProfiler) #library(tidyverse) combine.DE = readRDS("combine.aneuploid-vs-E.GLMM.DEG.result.rds") combine.DE = combine.DE[which(rowMax(as.matrix(combine.DE[,c("pct1","pct2")]))>0.1 & rowMax(as.matrix(combine.DE[,c("log.mean1","log.mean2")]))>0.1),] c...
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library(tidyverse) library(stringr) # load in pooled m6Anet dataset m6a_inf <- read_csv("results/methylation/m6anet/m6aNet_ctrl_te_inf//data.result.csv.gz") m6a_inf_pass <- m6a_inf %>% dplyr::filter(probability_modified > 0.9) # FBti to Transposon name index unique_table <- read_table("id_validation_table_20582.tx...
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library(R2nparray) x <- c(-50, 175, 149, 214, 247, 237, 225, 329, 729, 809, 530, 489, 540, 457, 195, 176, 337, 239, 128, 102, 232, 429, 3, 98, 43, -141, -77, -13, 125, 361, -45, 184) x <- ts(x, start=c(1951, 1), frequency=4) conv2 <- filter(x, c(.75, .25), method="convolution") conv1 <- filter(x, ...
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library(Matrix) plot_BC<-function(dna, rna, c1=1000, c2=1000, title=title){ layout(matrix(c(1,2,1,3),2,2,byrow=TRUE)) m<-merge(dna, rna, by="BC") plot(m[,2:3],pch=19, cex=0.1, log="xy", xlab="DNA reads", ylab="RNA reads", col="grey", xlim=c(1,100000), ylim=c(1,100000), main=title) lines(c(c1,c1),c(1,5000000), l...
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# scRNA-seq data processing and clustering library(Seurat) sce <- CreateSeuratObject(counts = counts, project = 'ICI', min.cells = 3, min.features = 200) sce[["percent.mt"]] <- PercentageFeatureSet(sce, pattern = "^MT-") sce <- subset(sce, subset = percent.mt < 25 & nFeature_RNA > 500 & nFeature_RNA < 7000) s_qc_list...
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# remove the expression values that have been imputed during integration with RPCA # take the positions (genes x cells) of the missing values of the counts layer of the RNA assay # remove the imputed values of the integrated assay at the same positions # takes a few minutes library(Seurat) dataPath = "data/" outfile...
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library(GenomicRanges) library(rtracklayer) library(VennDiagram) library(grid) library(tidyverse) ################################ # --- Load BED files as GRanges --- bed1 <- import("results/control_m6Anet_genomic_loci.bed", format = "BED") bed2 <- import("results/meclip_m6A_loci_sort.bed", format = "BED") bed3 <- i...
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--- title: "Length of stay in the ICU" author: "tom pollard" description: "Length of stay in the ICU for patients in MIMIC-III" output: pdf_document date: "10/10/2017" --- ```{r setup, include = FALSE} knitr::opts_chunk$set(echo = TRUE) library(ggplot2) library(RPostgreSQL) ``` ```{r dbconnect, include=FALSE} # Loa...
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library(tidyverse) library(broom) #' Perform fisher's exact test on 'summary count' df output by scripts/iclip_summarise_coverage.R counts_to_fisher <- function(data) { # Split data by region_type into a list of tibbles data_grouped <- data %>% group_by(region_type) region_groups <- data_grouped %>% ...
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#' Load BED file into memory #' #' @description Use Plink to convert BED to RAW then easily load it #' #' @return A data frame #' #' @author Luke Pilling #' #' @name load_bed #' #' @param in_bed A string. BED prefix #' @param verbose Logical. Be verbose (show individual steps), #' \code{default=FALSE} #' @param...
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args = commandArgs(trailingOnly=TRUE) source('Requirements/function.R') library(data.table) if (length(args)!=2){ print("Usage:file_path and norwayname must be provided") }else{ file_path <- args[1] norwayname <- args[2] rnaseq <- read.table(file_path,header = T,row.names = 1) selected_gene_lengt...
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source("M:\\josef_new\\eclipse_ws\\statsmodels\\statsmodels_py34_pr\\tools\\R2nparray\\R\\R2nparray.R") library('mgcv') library('gamair') d = data(mpg) #gam_a = gam(city.mpg ~ fuel + drive + s(weight,bs="cc",k=7) + s(hp,bs="cc",k=6), data = mpg, # sp = c(6.46225497484073, 0.81532465890585)) #gam_a = gam(...
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# »ñµÃËùÓÐpatternΪdownºÍupµÄdevCE£¬²é¿´exonÉÏÊÇ·ñ¸»¼¯SRSF1µÄ½áºÏmotif£¨hexamer£© # human_brain_down_devCE # human_brain_up_devCE setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) human_devAS <- read.table( file = "01-data/19-Development_alternative_splicing/human.devAS", sep = ",", ...
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# load packages require(Seurat) # load Seurat objects (list) sdata.batch <- readRDS('sdata_snRNA-seq_batch_SCT.rds') # output from 'SCTransform.R' # select integration features and prepare object features <- SelectIntegrationFeatures(sdata.batch) # default, nfeatures = 2000 sdata.batch <- PrepSCTIntegration(sdata....
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R
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library(tidyverse) library(vroom) library(slider) library(valr) # import variants variants <- vroom("data/external/strains/B6xCAST.snps_and_indels.merged.bed.gz") # get gene annotation - last GRCm38 version mart <- useEnsembl(biomart = "genes", dataset = "mmusculus_gene_ensembl", host="nov2020.arch...
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library(rtracklayer) library(GenomicRanges) setwd("/project2/xinhe/xsun/neuron_simulation/2.torus") df_snp <- readxl::read_xlsx("data/Bulk_NGN2_GABA_ASoC_for Torus.xlsx",sheet = 1) gr_hg38 <- GRanges(seqnames = paste0("chr",df_snp$CHR), ranges = IRanges(names = df_snp$ID, start = as.numeric(df_sn...
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fluidRow( box( width = 12, closable = F, title = tagList(icon("globe"), "World Map"), fluidRow( column( width = 8, fluidRow( column( width = 9, radioGroupButtons( inputId = "switchWorldMap", label = NULL, ...
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setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(ggplot2) library(ggsci) LCOR_pathogeicity <- read.table(file = "01-data/snp_pathogenicity_scatter_plot/LCOR_pathogenicity.bed") LCOR_pathogeicity <- LCOR_pathogeicity[, c(5, 13:14)] LCOR_pathogeicity <- LCOR_pathogeicity[!duplicated(L...
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library(KFAS) options(digits=10) # should run this from the statsmodels/statsmodels directory dta <- read.csv('datasets/macrodata/macrodata.csv') obs <- diff(data.matrix(dta[c('realgdp','realcons','realinv')])) obs[1:50,1] <- NaN obs[20:70,2] <- NaN obs[40:90,3] <- NaN obs[120:130,1] <- NaN obs[120:130,3] <- NaN mod...
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library(twice) library(dplyr) library(ComplexHeatmap) data("hg19rmsk_info") load("data/mayoTEKRABber_balance.RData") # load mayo RNA seq result c1_confirm <- read.csv("tables/hmc1_sig_forNetwork.csv") c2_confirm <- read.csv("tables/hmc2_sig_forNetwork.csv") confirm_mix <- c(c1_confirm$pair, c2_confirm$pair) confirm_m...
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# ============================================================================= # 配置文件:路径设置 # ============================================================================= # 注意:请根据您的实际环境修改以下路径 # 项目根目录 PROJECT_ROOT <- "." # 环境文件目录 ENV_DIR <- file.path(PROJECT_ROOT, "envs") # 数据目录 DATA_DIR <- file.path(PROJECT_ROOT, "...
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# �鿴other UCR��other RF��gwRVIS��û���������� setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(ggplot2) library(ggview) library(ggpubr) library(ggsci) library(ggbeeswarm) library(Hmisc) library(car) # other UCR gwRVIS -------------------------------------------------------- other_U...
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R
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# read in individual raw Seurat objects LV_5FBS <- readRDS(here::here("objects", "raw_seurat_objects", "lv_5FBS.rds")) LV_10FBS <- readRDS(here::here("objects", "raw_seurat_objects", "lv_10FBS.rds")) LV_15FBS <- readRDS(here::here("objects", "raw_seurat_objects", "lv_15FBS.rds")) Lv_2hpf <- readRDS(here::here("objec...
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library(feather) # RNA-seq data path data_loc = '/data/rnaseqanalysis/shiny/facs_seq/Mm_VISp_14236_20180912' # Annotation data anno_feather_path <- file.path(data_loc,'anno.feather') anno_feather_data <- read_feather(anno_feather_path) anno_feather_cols <- names(anno_feather_data) select_subclass_labels <- c("Vip", ...
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# Siwei 11 Mar 2019 # init library(Gviz) # data("cpgIslands") library(rtracklayer) library(BSgenome) library(BSgenome.Hsapiens.UCSC.hg38) library(TxDb.Hsapiens.UCSC.hg38.knownGene) library(ensembldb) library(org.Hs.eg.db) library(grDevices) library(gridExtra) ########## options(ucscChromosomeNames = F) plot_AsoC_fi...
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R
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library(tidyverse) ## Script to output selected target gene lists to file (supplementary data) load("processed/ferguson_hela/2023-11-29_hela_ko_tf_activity_gsea.Rdata") names(elk1_target_list) # All ELK1, unique ELk1, All ELK4, unique ELK4 lists_to_keep <- c("chipseq_hela_both_elk1", "chipseq_hela_unique_elk1", "ch...
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R
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# Load pre-calculated Seurat object #### library(Seurat) load("../data/stoeckius/CBMC.seurat.RData") # Load DCA denoised data #### dca <- read.csv("../data/stoeckius/stoeckius_dca.csv", row.names = 1) # Add imputed data to Seurat object #### tmp <- dca rownames(tmp) <- gsub("HUMAN", "IMPUTED", rownames(tmp))...
59c41ff93966b008a30ce6fc31c787109e1f6ca7cc55deb79c3bda4a4f877e48
R
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#function for analysis sweep_sparse = function(x, margin, stats, fun = "*") { #handle error if (!class(x)[1] %in% c("dgTMatrix","dgCMatrix","dgRMatrix","dgeMatrix","matrix")) { stop("Only several saprse matrix types are supported: dgTMatrix,dgCMatrix,dgRMatrix,dgeMatrix,matrix") } if((length(stats)!=1) &...
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library(mice) library(vegan) library(VIM) library(reshape) library(ggplot2) library("GUniFrac", lib="/usr/local/lib/R/site-library") library(rptR) mri <- read.csv("data/BIBAGO_MRI_dataset.csv") head(mri) # remove NA's mri <- mri[!is.na(mri$Subject),] mri <- mri[!is.na(mri$Pen),] pMiss <- function(x){sum(is.na(x))/l...
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R
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# load packages require(tidyverse) require(Seurat) require(monocle3) # load data sdata.align <- readRDS('sdata_align_pseudotime_minmin.rds') downsampled_matrix <- readRDS('downsampled_counts_matrix.rds') # load gene names table gene.names <- read_csv('gene_names.csv') # add downsampled counts, set default assay sdat...
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R
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--- title: "SpliceFlow" output: rmarkdown::html_vignette vignette: > %\VignetteIndexEntry{SpliceFlow} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} --- ```{r, include = FALSE} knitr::opts_chunk$set( collapse = TRUE, comment = "#>" ) require(dplyr) ``` ```{r setup} library(SpliceFlow) library(...
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R
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#-------- Download to local directory OR directly source PLACO+ from github # source("PLACO_v0.2.0.R") require(devtools) source_url("https://github.com/RayDebashree/PLACO/blob/master/PLACO_v0.2.0.R?raw=TRUE") #-------- set.seed(1) ## For an example, let's first simulate a toy set of GWAS summary ## statistics on 2 co...
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# setwd("/Users/yueliu/temp/temp1/Project_Diversity_pQTL/finemapping/MESuSIE/ENSG00000172803") args <- commandArgs(trailingOnly = T) library(tidyverse) library(MESuSiE) # args[1]="plink.race.list" race <- read.table(args[1], as.is = T)$V1 if (length(race) < 2) { cat("fewer than two races, quit\n") q() } if (lengt...
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################################################################################ ### LIBD pilot 10x snRNA-seq: Nucleus accumbens samples - 8 samples (as of revision) ### **Region-specific analyses** ### - R-batch job for detxn of optimal PC space with 'sce.nac' object ### -> see '10x_NAc-n8_step02_clust-ann...
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# load packages require(Seurat) # load Seurat objects (list) sdata.batch <- readRDS('sdata_batch_SCT.rds') # output from 'SCTransform.R' # select integration features and prepare object features <- SelectIntegrationFeatures(sdata.batch) # default, nfeatures = 2000 sdata.batch <- PrepSCTIntegration(sdata.batch, anc...
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R
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library(kronos) library(ggplot2) library(gridExtra) library(tidyverse) library(corrplot) library(gprofiler2) library(outliers) library(dplyr) library(readxl) setwd("/Users/mariareinacampos/Documents/00_MASTER/TFM/Resultats/Results_PD35_hpc") bigdata_pd35_hpc <- read_excel("/Users/mariareinacampos/Documents/00_MASTER/T...
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# This script was run to produce a lightweight SCE object, # subset to the top-25 mean-ratio markers and using a dense counts assay. The # goal is to make randomly subsetting and pseudobulking as fast as possible in # the following hspe and bisque scripts, which perform these operations up # to 1000 times li...
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# ²é¿´ncRNA UCRÔÚlncRNAÉϵÄλÖã¬ÊÇexon»¹ÊÇintron setwd(dir = "D:/R_project/UCR_project/") options(stringsAsFactors = FALSE) rm(list = ls()) library(tidyverse) library(ggprism) # 66¸öncRNA UCR£¬65¸öncRNA UCRºÍ56¸ölncRNAÖØµþ£¬uc.15ºÍmiRNA ENSG00000224592ÖØµþ ncRNA_UCR <- read.table(file = "02-analysis/16-New_classifi...
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R
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library(rtracklayer) library(GenomicRanges) setwd("/project2/xinhe/xsun/neuron_simulation/2.torus") df_snp <- readxl::read_xlsx("data/SNPs_for_Torus.xlsx",sheet = 1) gr_hg38 <- GRanges(seqnames = df_snp$CHROM, ranges = IRanges(names = df_snp$ID, start = as.numeric(df_snp$POS), end = as.numeric(df...
a85ada6de72bf8fc91a4e7e045fe9bf3f19fb8727fb01a09306c2d5db99ab880
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# Source: View(autoEstCont) PlotContaminationFraction <- function(object, contaminationRange = c(0.01, 0.8), title, verbose = TRUE) { dd <- object$fit$dd priorRho <- object$fit$priorRho priorRhoStdDev <- object$fit$priorRhoStdDev p.L = function(x, alpha) { if (x == 0) { 0 } else { qgamma...
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#!/usr/bin/env Rscript suppressPackageStartupMessages({ library(GenomicAlignments) library(dplyr, warn.conflicts = FALSE) library(tibble) library(tidyr) library(data.table) library(dplyr) library(optparse) }) # importing option_list = list( make_option(c("-i", "--input"), type="character", default=NUL...
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R
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#!/usr/bin/env Rscript suppressWarnings({ suppressPackageStartupMessages({ library(optparse) library(dplyr) library(bambu) }) options(dplyr.summarise.inform = FALSE) option_list = list( make_option(c("-b", "--bam"), type="character", default=NULL, help="merged bam file"), ...
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R
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# This script defines the create_binaryMtx function for gene-region analysis. # Function: create_binaryMtx # This function processes a data frame containing gene and region information # and generates a binary matrix where each row represents a gene and each column # represents a region. The matrix indicates the pre...
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R
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# this script checks VIFs for the linear terms in the models library(car) # version 3.1-3 # prepare df to store VIF results vifs <- data.frame(matrix(nrow = 5*7, ncol = 3)) # nrow = n_imputations * n_outcomes colnames(vifs) <- c("imp", "outcome", "max_VIF") vifs$imp <- rep(1:5, each = 7, length.out = 5*7) vifs$outcom...
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R
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setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(ggplot2) PBX3_exon_pathogenicity <- read.table(file = "01-data/snp_pathogenicity_scatter_plot/PBX3_pathogenicity.bed") PBX3_exon_pathogenicity <- PBX3_exon_pathogenicity[, c(8, 15:16)] PBX3_exon_pathogenicity <- PBX3_exon_pathogenicit...
dd24eaf9276ac8dbd2e9000fab545445c44ae381b8ac3d07cdccb0346d41d277
R
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context("compareRhythms_dodr") load("test_data_ma.rda") test_that("DODR analysis works for default params", { results <- compareRhythms(expr, exp_design, method = "dodr") expect_s3_class(results, "data.frame") expect_named(results, c("id", "category", "rhythmic_in_CC", "rhythmic_in_KD", "diff_rh...
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R
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options(stringsAsFactors = FALSE) library(ggplot2) library(reshape2) library(dplyr) library(stringr) library(lme4) library(lmerTest) library(RColorBrewer) library(ggpubr) #### SNV burden gyri vs. sulci #### df <- read.table("data/TableS3_PTA_burden.tsv", header=T, sep="\t") df$Case_ID <- as.character(df$Case_ID) sele...
aca0dd6c25a7b1866275e380b218650151a060454bb42428cd4b13d9d0203594
R
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install.packages("ggseqlogo") library(ggseqlogo) library(ggplot2) #A C G T data(ggseqlogo_sample) pfm <- matrix(c( 0.01 , 0.01 , 0.97 , 0.01 , 0.01 , 0.01 , 0.97 , 0.01 , 0.01 , 0.97 , 0.01 , 0.01 , 0.01 , 0.48 , 0.48 , 0.01 , 0.01 , 0.48 , 0.48 , 0.01 , 0.25 , 0.25 , 0.25 , 0.25 ), ncol = 4...
f4f9b0e4bacb1efee57e638eeb1b629300444d6a86dd6080809bd39cc32ce47e
R
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################################################################################ ### LIBD pilot 10x snRNA-seq: HPC samples ### **Region-specific analyses** ### - R-batch job for detxn of optimal PC space with 'sce.hpc' object ### -> see '10x_HPC-n3_step02_clust-annot_MNT.R' ### for setup of the S...
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R
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## estimate effect sizes from association tests of features ## se standard errors of the coefficients ## mat input data matrix (features x samples) ivwfe.stats <- function(estimate, se, mat=NULL, rho=NULL) { ## http://onlinelibrary.wiley.com/doi/10.1002/sim.6835/full ## calculate rho if (is.null(rho)) { ...
ae84360586003022c0d739574bde289b71c8665040f4675ac3ad6f723476e07a
R
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library(stringi) library(tidyverse) ### annoate flair diff module 5 output deGeneRes <- read_tsv("/Volumes/LaCie/DRS_compairison/results/flair/5_diff_3/isoforms_deseq2_tau_v_ctrl.tsv") deGeneRes <- read_tsv("/Volumes/LaCie/DRS_compairison/results/flair/5_diff_3/workdir/isoforms_deseq2_tau_v_ctrl_results.tsv") #split...
83d7fdac0c5c6c1f0829241781fb48a66c1976b9eb7c75c59113f71a7f978efc
R
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# rfÏà¹ØµÄ·ÖÀà setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(dbplyr) library(tidyverse) coding_rf <- read.table(file = "02-analysis/16-New_classification/01-rf_form_protein_coding_gene.bed", sep = "\t") ncRNA_rf <- read.table(file = "02-analysis/16-New_classification/02-rf...
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R
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#' Tophat #' #' Align reads to genome using Tophat2. The Script creates new directory called BAM in the same path were the FASTQ dir is located. #' @param Directory The Path of the directory were the FASTQ files are #' @param Library_Type "Paired" or "Single" #' @param Threads Number of threads used for the alignment #...
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R
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#' Add gene_id and gene_name #' #' @param annotation ENSEMBL annotation in granges #' #' @return trimmed exon #' @export #' @import dplyr #' @importFrom magrittr %>% #' @importFrom dplyr left_join #' @examples addGeneName <- function(annotation, intron_database) { gene_to_gene_name_map <- annotation[annotation$type =...
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R
1,833
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#' Dataset Metric #' @description #' Class to provide access to functions for computing metrics on an aif360 compatible dataset #' #' @param data A aif360 compatible dataset #' @param privileged_groups Privileged groups. List containing privileged protected attribute name and value of the privileged protected attribut...
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R
1,833
41
#Make plots ############################################################################################### #Load libraries library(Seurat) library(tidyverse) #Create directory to store plots dir.create("4_plots_mixed_1.5_2M", showWarnings=T) #Load mapped Seurat data with reductions df = LoadSeuratRds("3_integrated_...
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#' @title coxvolcano #' perform volcanoplot between beta coefficient and p-values from Cox #' @param df results coxbycol function #' @param font.size size of font in the graph #' @usage library(loopcolcox) #' @usage data(cancer) #' @usage library(dplyr) #' @usage cancer%>%select(3:7)->data #' @usage df<-coxby...
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R
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setwd(dirname(rstudioapi::getActiveDocumentContext()$path)) list2env(rjson::fromJSON(file = "00_configs.json"), envir = .GlobalEnv) library(Seurat) library(qs) library(ShinyCell) objects_folder <- file.path(project_folder, "objects", "R", "seurat") ca_folder <- file.path(project_folder, "objects", "R", "clustassess") ...
a0a9d64d0401269c211bbded9adf1e04a1d511c57850ef00f8344ae50c6d6879
R
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library(plotly) # Author: Sean Maden # # Make 3d density plots from HALO coordinates, DAPI signal intensities from an # example slide. # load data dpath <- file.path("HALO", "Deconvolution_HALO_analysis") fnv <- list.files(dpath, recursive = T) fnv <- fnv[grepl("\\.csv$", fnv)] csvi <- read.csv(file.path(dpath, fnv[1...
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R
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#Once you build your network with metacore see "Build Network using Metacore pdf" (building algorithm: direct interaction) you can run this script #get the xls file from Metacore for mapepd and interaction convert them to csv file and proceed with the script library(tidyverse) cells=c("Astrocyte","Neurons")#First...
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R
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library(scrattch.vis) library(feather) library(tidyverse) options(stringsAsFactors = F) # Setting working directory ----------------------------------------------- this.dir <- dirname(parent.frame(2)$ofile) setwd(this.dir) # Data paths -------------------------------------------------------------- project_path <- ...
d1ef9be335bb7f1a83391e2c865577cc4b05f2a3899213d572c8c5afabf1a0e5
R
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## 1. WGCNA - soft thresholding ## library(WGCNA) library(data.table) library(cluster) library(data.table) #1. Load data used in GPMethylation ============================================================================================= load(paste0(dataPath, "FetalBrain_Betas_noHiLowConst.RData")) betas <- betas.d...
89ef2fdcac13f715fa03bf4d9d6dfdaa3210c9a5c87b9f67052cc36ece5bc0ce
R
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setwd(dirname(rstudioapi::getActiveDocumentContext()$path)) list2env(rjson::fromJSON(file = "00_configs.json"), envir = .GlobalEnv) library(Seurat) library(Signac) library(ClustAssess) library(qs) library(rhdf5) library(dplyr) library(future) library(doParallel) # 50 GB options(future.globals.maxSize = 50 * 1024^3) pl...