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#### library(readxl) library(jaffelab) library(readr) library(pracma) ## read in reference data ref = read_csv("raw_data/square_MSN_exp_rnascope.csv") ref = as.data.frame(ref[,1:5]) ref_mat = as.matrix(ref[,2:5]) rownames(ref_mat) = ref$Population ref_mat = ref_mat[,c(1,2,4,3)] ## read in long data dat =read.csv("raw...
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library(CellChat) library(patchwork) options(stringsAsFactors = FALSE) s_qc.combined_sub <- subset(s_qc.combined,Doublets=='N') data.input =GetAssayData(object =s_qc.combined_sub,slot = 'data') # normalized data matrix meta = s_qc.combined_sub@meta.data # a dataframe with rownames containing cell mata data cell.use_CR...
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rm(list=ls()) set.seed(123) source('load_libraries.R') source('aux_functions.R') library(biomaRt) # Function to detect delimiter detect_delimiter = function(filepath) { # Read the first line of the file first_line = readLines(filepath, n = 1) # Check for common delimiters if (grepl("\t", first_line)) { ...
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# Siwei 09 Aug 2023 # Siwei 05 Jul 2023 # plot 1MB proximal region of rs1532278 (CLU) # chr8:27608798 # init ##### library(Gviz) library(rtracklayer) library(BSgenome) library(BSgenome.Hsapiens.UCSC.hg38) library(TxDb.Hsapiens.UCSC.hg38.knownGene) library(ensembldb) library(org.Hs.eg.db) library(grDevices) library(g...
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#!/usr/bin/env Rscript #### Fine cell type annotation of B cell subset for MBM_sc #### Author: Jana Biermann, PhD library(dplyr) library(Seurat) library(ggplot2) library(gplots) library(viridis) '%notin%' <- Negate('%in%') path.ct <- 'data/cell_type_DEG/MBM_sc/bcells/' filename <- 'MBM_sc_bcells' seu <- readRDS('da...
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################################################################################ ### LIBD 10x snRNA-seq [pilot] revision (n=10) ### STEP 01.batchJob: Read in SCEs and perform nuclei calling (`emptyDrops()`) ### Initiated: MNT 25Feb2021 ################################################################################ li...
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# FFERREIRA 12/26/2024 # Sanford Consortium - UCSD # Prepares table to export as SUP table # Loads LIBs library(tidyverse) # Sets WD wd <- getwd() setwd(wd) # OBJs to read INFILE g1 <- "10" # Treatment g2 <- "CTRL" # Control c1 <- "C136" # cell lineage #1 c2 <- "NOVA1-C15" # cell lineage #2 batch <- "batch2" # ...
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--- output: github_document --- <!-- README.md is generated from README.Rmd. Please edit that file --> ```{r, include = FALSE} knitr::opts_chunk$set( collapse = TRUE, comment = "#>", message = FALSE, fig.path = "man/figures/README-", out.width = "100%", warning = FALSE ) ``` ```{r library, echo=FALSE} li...
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setwd(dirname(rstudioapi::getActiveDocumentContext()$path)) list2env(rjson::fromJSON(file = "00_configs.json"), envir = .GlobalEnv) library(ClustAssess) library(Seurat) library(ggplot2) library(dplyr) library(reshape2) ca_folder <- file.path(project_folder, "objects", "R", "clustassess") ca_app_folder <- file.path(pro...
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## Principal Component Analysis (PCA) on FANS lifecourse DNAm dataset ## library(ggplot2) library(data.table) # for fread library(RColorBrewer) library(viridis) library(scales) # for rescale library(pscl) # for pR2 celltype_cols <- c(plasma(4)[2],viridis(4)[3]) # plasma(4)[2] = #9C179EFF = purple; viridis(4)[3] = #...
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setwd("Documents/ZS/NuevasImagenes/") # Upload ZS Revelen disease/pathway/annotation table diseaseExp <- read.csv("~/Documents/ZS/NuevasImagenes/exploratory_reshaped_disease.csv") data <- data.frame(diseaseExp) library(dplyr) library(ggplot2) library(stringr) library(tidyr) # Group by annotation and calculate the ...
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#' Translate gene ids of a given dgeMatrix of seismic specificity scores from a given organism (e.g., mouse) #' to another (e.g., human) based on orthology. #' #' Currently supported organisms: hsa, mmu (human, mouse, respectively) #' Currently supported IDs: symbol, ensembl, entrez #' #' Unmatched rows are dropped fro...
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library(tidyverse) #' Prepare Heatmap Dataframe #' #' Prepare a dataframe containing sample-wise PAS usage differences for plotting as a heatmap of samples (x) * events (y) #' #' @param df A dataframe containing sample-wise differences in PAS usage, (e.g. `ppau_delta_paired_cryp`). #' @param le_id_order A vector speci...
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######## #library(SCnorm) library(lmerTest) library(SingleCellExperiment) library(data.table) library(emmeans) library(dplyr) library(reshape2) args = commandArgs(trailingOnly = TRUE) path = args[1] norm.sce = args[2] meta.data = args[3] chrom.state = args[4] #####loading normalized data and cell meta infor norm.sce...
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# ±È½Ï²»Í¬Àà±ðµÄUCRÉϵÄGCº¬Á¿ setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(Biostrings) library(ggplot2) library(ggpubr) # get ucr type load("D:/R_project/UCR_project/02-analysis/12-karyoplote/UCR_type_gene.Rdata") # get ucr sequence ucr_sequence <- readDNAStringSet(filepath = ...
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# ¸ù¾ÝUCRµÄ»ùÒò×éλÖöÔUCR½øÐзÖÀ࣬²¢Ñ°ÕÒ×î½üµÄ»ùÒòÓÃÓÚÏÂÓεĸ»¼¯·ÖÎö rm(list = ls()) setwd(dir = "D:/R_project/UCR_project/") library(tidyverse) UCR_with_overlap_ratio <- read.table(file = "data/0A-EvolutionAnalysisData/UCR classification/UCR_with_overlap_ratio.bed", sep = "\t...
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# The original version should do some manually working # With more understandings about dbsnp (https://www.ncbi.nlm.nih.gov/snp/) and ensembl (http://rest.ensembl.org/), I re-wrote the function # The function provides two options for database, one for dbsnp, another for ensembl. I prefer dbsnp and add additional functi...
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#!/usr/bin/env R # Author: Sean Maden # # Summarize sce data by donor for ROSMAP sce object. # # #---------- # load data #---------- sce.fpath <- file.path("rosmap_snrnaseq", "sce_all_rosmap-original.rda") sce <- get(load(sce.fpath)) # get metadata cd <- colData(sce) #------------------------------------------------...
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context("compareRhythms_limma") load("test_data_ma.rda") exp_design_batch <- cbind(exp_design, batch = ifelse(seq(nrow(exp_design)) %% 2, "a", "b"), stringsAsFactors=TRUE) test_that("limma analysis works for default params", { results <- compareRhythms(expr, exp...
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# This is a copy of the original code from the standard version of the # sva package that can be found at # https://bioconductor.org/packages/release/bioc/html/sva.html # The original and present code is under the Artistic License 2.0. # If using this code, make sure you agree and accept this license. library(matrix...
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## Loading libraries library(dplyr) library(data.table) library(nlme) ## Loading data data_base.a <- readRDS(paste0(directory,"DLPFC_matched_cross_autosome.rds")) # cn: 480 rows 17945 columns # dlpfc: 622 rows, 17105 cols # pcc: 388 rows, 18402 cols # name corresponding tissue to whichever data loaded #tissue <- "D...
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#prepare files for scDRS/FUMA/MAGMA ##### 1. load packages and data####### ###load packages if (!require("here")) { install.packages("here") library("here") } if (!require("magrittr")) { install.packages("magrittr") library("magrittr") } if (!require("tidyverse")) { install.packages("tidyverse") library(...
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# library(gtools) # library(data.table) # # 統合部分 ===== # pcrByRegion <- fread(file = paste0(DATA_PATH, "MHLW/pcrByRegion.csv")) # # detailByRegion <- fread(paste0(DATA_PATH, "detailByRegion.csv")) # detailByRegion[, 都道府県名 := gsub("県|府", "", 都道府県名)] # detailByRegion[, 都道府県名 := gsub("東京都", "東京", 都道府県名)] # # detailByRe...
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setwd("osmFISH_AllenSSp/") library(liger) library(hdf5r) library(methods) # allen VISp allen <- read.table(file = "data/Allen_SSp/SSp_exons_matrix.csv", row.names = 1, sep = ',', stringsAsFactors = FALSE, header = TRUE) allen <- as.matrix(x = allen) Genes_count = rowSums(allen > 0) alle...
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library(sscVis) library(data.table) library(grid) library(cowplot) library(ggrepel) library(readr) library(plyr) library(ggpubr) library(ggplot2) library(tidyverse) ############################################################################################ # Useful function do.tissueDist <- function(cell...
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#' Population priors #' #' This script estimate the template of population priors on DHCP data (2nd release) #' Should run in parallel by: rel2_estimate-template_term.py #' #' #' @author Diego Derman, FRG - IUB #' v1: 2021-08-12 #' v2: 2022-02-01 #' v3: 2022-02-16 #' duration: 7 minutes for 35 subjects, using 48 thre...
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library("slurmjobs") library("tidyverse") library("here") #### slurmjob setup #### hvg_prop <- as.character(seq(10,100, 10)) # hvg_files <- sprintf("../../processed-data/06_marker_genes/09_HVGs/HVG%d0.txt", seq(1,10)) # all(file.exists(hvg_files)) ## 1 01_deconvolution_Bisque # job_loop(loops = list(HVG=hvg_prop), ...
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pred.vizB <- function (cur_pat, act=F, labs=F){ # Design B: Plots scaled ystar density distributions on inside of ARAT recovery plot. # Arguments: # <cur_pat> an integer specifying the patient number # Requires: # <pred_xgb> data frame with bootstrap prediction results (output predict.XGB) # <dat_p...
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setwd(dirname(rstudioapi::getActiveDocumentContext()$path)) list2env(rjson::fromJSON(file = "00_configs.json"), envir = .GlobalEnv) library(Seurat) library(qs) library(future) library(foreach) library(dplyr) library(EnsDb.Hsapiens.v86) library(gprofiler2) objects_folder <- file.path(project_folder, "objects", "R") so_...
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library(DESeq2) library(ISoLDE) # Read Data --------------------------------------------------------------- # DDS object with allele-specific counts at the gene level dds <- readRDS("results/DESeqDataSet/dds_gene_allele.rds") # Prepare Data for ISolDE ------------------------------------------------- # ISoLDE has ...
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library("SingleCellExperiment") library("BisqueRNA") library("here") library("sessioninfo") ## get args args = commandArgs(trailingOnly=TRUE) marker_label <- args[1] marker_file <- NULL if(marker_label == "FULL"){ message("Using FULL gene-set") } else { marker_file <- args[2] stopifnot(file.exists(marker_file)...
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# Reference values for PyMARE's permutation test. # # Writes pymare/tests/data/metafor_permutest_reference.json, which # pymare/tests/test_metafor_permutest.py reads. Run it through the harness in # this directory rather than directly, so the R and metafor versions are the # pinned ones: # # validation/metafor/rege...
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######## #library(SCnorm) library(lmerTest) library(SingleCellExperiment) library(data.table) library(emmeans) library(dplyr) args = commandArgs(trailingOnly = TRUE) path = args[1] norm.sce = args[2] meta.data = args[3] chrom.state = args[4] #####loading normalized data and cell meta infor norm.sce = readRDS(norm.sc...
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library(DESeq2) library(ISoLDE) # Read Data --------------------------------------------------------------- # DDS object with allele-specific counts at the gene level dds <- readRDS("results/DESeqDataSet/dds_gene_allele.rds") # Prepare Data for ISolDE ------------------------------------------------- # ISoLDE has ...
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# R code to dump fair test dataset to # checking compatibility. # # Usage: # Rscript test_get_R_tweedie_var_weight.R > res_R_var_weight.py cat("# This file auto-generated by test_get_R_tweedie_var_weight.R\n") cat(sprintf("# Using: %s\n", R.Version()$version.string)) cat("import numpy as np\n") cat("import pandas as ...
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# ·ÖÎöһϻ®ºÛʵÑéºÍtranswellʵÑéµÄ½á¹û setwd(dir = "D:/R_project/UCR_project/") options(stringsAsFactors = FALSE) rm(list = ls()) library(tidyverse) library(readxl) library(ggplot2) library(reshape2) library(ggprism) library(ggview) library(ggsignif) # caki1 ---------------------------------------------------------...
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# Author: Lauren Rylaarsdam, PhD # 2024-2025 ############################################################################################################################ #' @title indexChr #' @description If the whole hdf5 file had to be searched for relevant reads #' every time gene-specific methylation information wa...
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# Load required libraries library(dplyr) library(ggplot2) library(gridExtra) library(ggdist) library(RColorBrewer) # Define color palette globally my_colors <- brewer.pal(12, "Set3") # ============================================ # Data Preparation # ============================================ # Load data data <- r...
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# Siwei 16 Jun 2023 ##### # plot a large PCA include MG, Ast, GA, and possibly NGN2 # ATAC-Seq data using the count matrix of Hauberg ME et al. (GSE143666) # need to add data from Kosoy R et al. (syn26207321) # init ##### library(readr) library(edgeR) library(Rfast) library(factoextra) library(Rtsne) library(irlba) ...
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## Load plink before starting R: # module load plink/1.90b6.6 ## Also load twas fusion code # module load fusion_twas/github library("SummarizedExperiment") library("sessioninfo") library("getopt") library("BiocParallel") library("data.table") ## Flags that are supplied with RScript spec <- matrix(c( "cores", "c"...
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library(ComplexHeatmap) library(ggplot2) library(ggpubr) # import brain data read_rds_files("../../paper_source_code/data/results_rdata/") c1_kznfs <- unique(HmPtC1$corrRef$geneName) #285 c1_kznfs_res <- HmPtC1$DEobject$gene_res %>% data.frame() %>% filter(log2FoldChange <= -1.5 & pvalue < 0.05) select_kznfs...
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library(DESeq2) library(ISoLDE) # Read Data --------------------------------------------------------------- # DDS object with allele-specific counts at the gene level dds <- readRDS("results/DESeqDataSet/dds_isoform_allele.rds") # Prepare Data for ISolDE ------------------------------------------------- # ISoLDE h...
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# FFERREIRA 03/24/2024 # Nina Project - Neanderthal # Merges the MAP file for PC-Genes only created in the previous R code with TXimport data # Sets WD wd <- getwd() setwd(wd) # General OBJs sep <- "\t" nas <- c("NA", "", NA) myComps <- c("ASC-10_vs_ASC-30", "ASC-10_vs_ASC-CTRL", "ASC-30_vs_ASC-CTRL", "...
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#this function takes the total rna tables produced by featureCounts, and gives a reasonable output data frame and #metadata frame make_deseq_dfs = function(total_table, grep_pattern = "", leave_out = "", base_grep = "", contrast_grep = ""){ if(grep_pattern == ""){ grep_pattern = glue::glue("{base_grep}|{contras...
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library("SummarizedExperiment") library("tidyverse") library("here") library("sessioninfo") #### Set up #### ## dirs plot_dir <- here("plots", "09_bulk_DE", "01_bulk_data_exploration") if(!dir.exists(plot_dir)) dir.create(plot_dir, recursive = TRUE) ## load colors load(here("processed-data", "00_data_prep", "bulk_c...
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library(tidyverse) library(tidytext) # read in kmer distribution tables for all experiments dbrn_paths <- list.files(path = "data/peka_qapa", pattern = "_6mer_distribution_genome.tsv$", recursive = T, full.names = T) %>% set_names(str_remove(...
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#### Figure S5C ##### #### Expression of genes associated with perisynaptic astrocytic processes #### library(matrixStats) library(gplots) library(stringr) library(here) # human astrocyte DEGs: h_c <- read.table(here("data/astrocytes", "Astro_human_vs_chimp_sig_genes.csv"), sep=",", header=TRUE) h_c <- h_c[h_c$padj<...
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#!/usr/bin/env Rscript #### Fine cell type annotation of CNS and stromal cell subset for MBM_sn #### Author: Jana Biermann, PhD library(dplyr) library(Seurat) library(ggplot2) library(gplots) library(viridis) path.ct <- 'data/cell_type_DEG/MBM_sn/cns/' filename <- 'MBM_sn_cns' seu <- readRDS('data/cell_type_DEG/MBM...
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#!/usr/bin/env Rscript # FFERREIRA 03/24/2024 # Nina Project - Neanderthal ## Aggregates gene-level COUNTS / get TPM values ################ # 0. SETS UP ENV ################ # Loads libraries cat(paste("Loading libraries...\n", sep = "")) library(tximport) library(readr) #library(icesTAF) # Only necessary to perfo...
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# fig 3D library(ComplexHeatmap) library(TEKRABber) library(twice) library(tidyverse) load("data/primateBrainData.RData") data("hmKZNFs337") data("hg19rmsk_info") # prepare datasets including raw counts of KRAB-ZNFs and TEs # convert to TPM # genes df_hm_gene <- hmGene[,c(-1)] rownames(df_hm_gene) <- hmGene$geneID # ...
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# libraries --------------------------------------------------------------- library(Seurat) library(tidyverse) library(GGally) library(cowplot) library(ComplexHeatmap) library(scales) library(circlize) library(DESeq2) # read in the final object ------------------------------------------------ # scobj <- readRDS("../.....
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library(ggplot2) # Author: Sean Maden # # Smooths of DAPI signal by marker signal for each slide. Makes smooths (using # geom_smooth) of DAPI x Marker signal plots, grids each slide with facet_wrap(). # #---------- # load data #---------- dpath <- file.path("HALO", "Deconvolution_HALO_analysis") fnv <- list.files(dpa...
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library(SingleCellExperiment) exprMat <- as.matrix(GetAssayData(object = tmp, slot = "data")) cellInfo <- colnames(x = tmp) cellInfo <- data.frame(seuratCluster=Idents(tmp)) library(SCENIC) db='C:/Users/nelso/Downloads/Ristarget/' list.files(db) scenicOptions <- initializeScenic(org="mgi", dbDir= db , nCores=10) sav...
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# Siwei 02 Feb 2024 # Plot Jubao's Diseases bar plot # init #### library(readxl) library(reshape2) library(plyr) library(ggplot2) library(scales) library(RColorBrewer) # load data ##### df_raw <- read_excel("Jubao_Diseases_bar_plot_v2.xlsx") df_list_by_cell_type <- split(x = df_raw, f = df_raw$Cell_Ty...
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# Script for combing all risk gene TPM counts and performing PCA (Supplementary Figures). Written by: J Gleeson (2023) # Directory containing IsoLamp output files for each gene ending in '_TPM_values.csv' setwd("<path to directory here>/PCA_data/") library(gghalves) library(ggplot2) library(ggfortify) library(data.ta...
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#' Siwei rewrite 18 Oct 2023 #' CreateVCF #' Since the bams output from bowtie2 pipeline has been added for readgroups, #' sorted, and indexed, skip the first three steps #' #' #' Create the VCF (Variant Call Format) file #' @param Directory The Path of to the BAM Directory #' @param Genome_Fa Path for whole genome FAS...
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library("SingleCellExperiment") library("BisqueRNA") library("here") library("sessioninfo") ## get args args = commandArgs(trailingOnly=TRUE) marker_label <- args[1] marker_file <- NULL if(marker_label == "FULL"){ message("Using FULL gene-set") } else { marker_file <- args[2] stopifnot(file.exists(marker_file)...
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#!/usr/bin/env Rscript #### Integration analysis: LISI plots #### Author: Jana Biermann, PhD library(Seurat) library(dplyr) library(ggplot2) library(gplots) library(ggrastr) library(viridis) library(scales) colBP <- c('#A80D11', '#008DB8') colSCSN <- c('#E1AC24', '#288F56') ### Select one label label <- 'MBPM_scn' ...
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#' Plot influential genes for a given trait and cell type after running find_inf_genes(). #' #' @param inf_df A data.frame or data.table of influential gene scores output #' by seismic. Must contain columns that correspond to genes, seismic #' specificity scores, MAGMA trait z-scores, dfbeta values, and a Boolean #' co...
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#### =============================================================== #### monocle2 #### =============================================================== #### Load Packages library(monocle) library(dplyr) library(Seurat) library(patchwork) library(ggsci) library(harmony) library(ggplot2) library(ggsci) options(mc.cores...
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# Siwei 13 Sept 2023 # make trace plot for Hanwen # compensate for background quenching effect # init #### library(readxl) library(baseline) library(gWidgets2) library(tidyr) library(ggplot2) library(RColorBrewer) # load data #### Stimulation_trace_signal <- read_excel("Stimulation_traces-7hr-013123.xlsx", ...
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# ʹÓÃeasyTCGA¶ÔncRNAÏà¹ØµÄlncRNA½øÐзº°©·ÖÎö setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(easyTCGA) library(stringr) library(ggplot2) library(forcats) library(ggview) # °²×°easyTCGA -------------------------------------------------------------- BiocManager::install("TCGAbioli...
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# ====PCR検査数の推移図データセット==== pcrData <- reactive({ dt <- rbind( dailyReport[, .( 日付 = date, 国内 = pcr.d, チャーター便 = pcr.f, 空港検疫 = pcr.x, クルーズ船 = pcr.y )], cbind( mhlwSummary[日付 > "2020-05-08" & 分類 == 0][, .( 国内 = sum(検査人数), ...
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# 查看NUCR-ncRNA在神经系统不同脑区的表达 setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(ggplot2) library(pheatmap) # get GTEx data load("D:/R_project/UCR_project/02-analysis/22-Tissue_specificity_across_different_organs/GTEx_exp_tau_normalized.Rdata") # get brain data brain_expr <- GTEx_exp_ta...
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library(ggplot2) library(dplyr) library(ggrepel) # Read data data <- read.table("D6_BMP.txt", header = TRUE, row.names = 1) neuron_associated_genes <- read.table("Dev_genes_associated_list.txt", header = TRUE) # Calculate the mean FPKM for DKO and WT data$mean_fpkm_DKO <- rowMeans(data[, c("DKO_F12_B_D6"...
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## Analysis of Age linear regression in bulk fetal cortex ## library(data.table) library(ggplot2) library(cowplot) library(gridExtra) library(viridis) library(plyr) # for ddply '%ni%' <- Negate('%in%') # not in source(paste0(scriptsPath, "fetal_plotFunctions.r")) #1. Load betas =================================...
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setwd("~/Documents/mixOmics/") ##### Load packages ##### library(mixOmics) library(readxl) library(readr) library(dplyr) library(foreach) library(doParallel) # Importing groups of samples groups <- as.data.frame(read_excel("~/Documents/mixOmics/Tablas/Groups_full.xlsx", sheet = ...
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# ¶Ô֮ǰµÄÎïÖֵıȶԽá¹ûÒ²½øÐÐеÄidentity¼ÆËã setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(stringr) UCR_location <- read.table(file = "01-data/UCR_raw/UCR_location_refseqid.txt", sep = "\t", header = TRUE) UCR_length <- UCR_location[, c(4:5)] # human -------------------------...
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# install.packages("hspe_0.1.tar.gz") library("hspe") library("SingleCellExperiment") library("jaffelab") library("spatialLIBD") library("here") library("sessioninfo") ## get args args = commandArgs(trailingOnly=TRUE) marker_label <- args[1] marker_file <- NULL ## not using txt list of marker genes, methods needs n...
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library("SingleCellExperiment") library("BisqueRNA") library("here") library("sessioninfo") ## get args args = commandArgs(trailingOnly=TRUE) marker_label <- args[1] marker_file <- NULL if(marker_label == "FULL"){ message("Using FULL gene-set") } else { marker_file <- args[2] stopifnot(file.exists(marker_file)...
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# Siwei 01 Feb 2024 # Plot Jubao's Diseases line graph # init #### library(readxl) library(reshape2) library(ggplot2) library(scales) library(plyr) library(RColorBrewer) # load data ##### df_raw <- read_excel("Jubao_Diseases_plot_line_graph.xlsx") df_melt <- reshape2::melt(data = df_raw, id ...
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--- title: "EIB_behavioural_ReactionTimes" author: "Barbara Cassone" date: "AUTOMATIC" output: html_document --- ```{r echo=FALSE, message=FALSE} if(!require("pacman")) install.packages("pacman") library(pacman) p_load("reshape2","ez","dplyr","lme4","lmerTest", "rmarkdown", "lattice", "ggplot2", "emmeans") ...
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#!/usr/bin/env R # # # # library(ggplot2) #------- # params #------- celltype.varname <- "cellType_broad_k" #---------- # set paths #---------- load.dpath <- save.dpath <- "Human_DLPFC_Deconvolution/processed-data/004_marker-gene-expr" # upset plot datasets # withdrop upsetdata.withdrop.fpath <- file.path(save.dpat...
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################################################################################ # Function to plot regional brain maps from .csv files ################################################################################ # Copyright (C) 2024 University of Seville # # Written by Natalia García San Martín (ngarcia1...
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# ---------------------------------------------------------------------------- # Libraries and setup ---- library("argparse") library("ggplot2") library("tidyverse") library("scales") library("Seurat") # Command line arguments ---- parser <- ArgumentParser(description = "Differential expression analyses") parser$add...
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setwd("osmFISH_Ziesel/") library(liger) library(hdf5r) library(methods) # Zeisel SMSC Zeisel <- read.delim(file = "data/Zeisel/expression_mRNA_17-Aug-2014.txt",header = FALSE) meta.data <- Zeisel[1:10,] rownames(meta.data) <- meta.data[,2] meta.data <- meta.data[,-c(1,2)] meta.data <- as.data.frame(t(meta.da...
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#----02_variables_and_exp_info_v01---------------------------------------------- #------------------------------------------------------------------------------- # Locomotor activity analysis for Reinhard et al. 2025 (10.1073/pnas.2506164122) # # Requirements: # 1)scripts: # 01_setup_v01 # 2)variables: ...
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# # file paths ################################################ # ukbrapr_paths = data.frame( object=c( "hesin", "hesin_diag", "hesin_oper", "gp_clinical", "gp_scripts", "death", "death_cause", "selfrep_illness", "cancer_registry", "baseline_dates"), path=c( "ukbrapr_data/hesin.tsv", "ukbrap...
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################################################################################ ### LIBD 10x snRNA-seq pilot (n=14) re-processing (Bioc v3.12) ### STEP 01.batchJob: Read in SCEs and perform nuclei calling (`emptyDrops()`) ### Initiated: MNT 03Mar2021 ####################################################################...
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#' Siwei rewrite 18 Oct 2023 #' SplitNCigarBam #' Since the bams output from bowtie2 pipeline has been added for readgroups, #' sorted, and indexed, skip the first three steps #' #' #' Create the VCF (Variant Call Format) file #' @param Directory The Path of to the BAM Directory #' @param Genome_Fa Path for whole genom...
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# load packages require(tidyverse) require(Seurat) require(phateR) # version 1.0.7 require(princurve) require(scales) # load Seurat object sdata.align <- readRDS('sdata_align_RefF_label.rds') # get PCA cells.use <- filter(sdata.align@meta.data, compute_phate) %>% rownames() dims.use <- 1:50 pca.embedding <- Embeddin...
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# AIM --------------------------------------------------------------------- # this is the initial step for the counting of senescent cells. In this step I generate the signatures score. # libraries --------------------------------------------------------------- library(tidyverse) library(ggrepel) library(lemon) # rea...
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--- title: "R Notebook of rV2 manuscript figure 2C" output: html_notebook --- ```{r Packages, echo=FALSE} library(tidyverse) library(Seurat) library(Signac) library(qs) library(rtracklayer) library(gUtils) source("AuxFunctions.R") ``` ```{r Set parameters} cores <- 6 ``` ```{r Load qs object} rV2.data <- qread("../s...
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suppressMessages(library(Seurat, quietly = T)) suppressMessages(library(ggplot2, quietly = T)) suppressMessages(library(Rtsne, quietly = T)) normalize <- function(x) { sf <- rowSums(x) sf <- sf / median(sf) x <- x / sf x <- log(x+1) scale(x, center = T, scale = T) } `%+%` <- paste0 args <- commandArgs(trai...
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--- title: "R Notebook of rV2 manuscript figure S3A" output: html_notebook --- ```{r Packages, echo=FALSE} library(tidyverse) library(Seurat) library(Signac) library(qs) library(rtracklayer) library(gUtils) source("AuxFunctions.R") ``` ```{r Set parameters} cores <- 6 ``` ```{r Load qs object} rV2.data <- qread("../...
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--- title: "R Notebook of rV2 manuscript figure S3A" output: html_notebook --- ```{r Packages, echo=FALSE} library(tidyverse) library(Seurat) library(Signac) library(qs) library(rtracklayer) library(gUtils) source("AuxFunctions.R") ``` ```{r Set parameters} cores <- 6 ``` ```{r Load qs object} rV2.data <- qread("../...
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# ¿´human intergenic ucrÔÚmouseºÍratÀïÊDz»ÊÇÒ²ÊÇintergenic£¬closest protein coding geneÊDz»ÊÇÒ²¸»¼¯ÔÚbrain development setwd(dir = "D:/R_project/UCR_project/") rm(list = ls()) library(tidyverse) library(ggplot2) # get intergenic ucr ucr_mouse <- read.table( file = "01-data/31-Intergenic_is_intergenic_in_mouse_rat/...
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setwd(dirname(rstudioapi::getActiveDocumentContext()$path)) list2env(rjson::fromJSON(file = "00_configs.json"), envir = .GlobalEnv) library(Seurat) library(dplyr) library(ggplot2) library(ClustAssess) library(qs) objects_folder <- file.path(project_folder, "objects", "R") ca_folder <- file.path(objects_folder, "clusta...
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pred.viz.mm <- function (cur_pat){ # Plots mm recovery pathway for grid plotting # Arguments: # <cur_pat> an integer specifying the patient number # Requires: # <M_mm> a model object containing the trained mixed effects model # <eval_mm> a dataframe with the available measurement at the m...
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library(ggplot2) library(data.table) library(Seurat) library(patchwork) library(scater) # QC replicates from each pool and merge for(i in 17:17) { print(i) data <- Read10X(data.dir = paste0("cellranger-count/Pool-", i, "-1/")) seurat_object_1 <- CreateSeuratObject(counts = data, project = paste0("Pool...
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loadMicrobiomeDataForPipeline <- function(fluxAll) { #' This function loads, processes, and filters microbiome data for use in a pipeline, #' ensuring compatibility with other datasets such as fluxes and metadata. The #' microbiome data includes species abundance, taxonomic classifications (Phylum, Class, #' Order, ...
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# snpEFF¶ÔUCRÉϵÄSNPµÄ×¢Êͽá¹ûÖ»ÓÐ138¸öHIGHÌ«µÍÁË£¬ÏÖÔÚÓÃAlphaMissenseÀ´ÊÔÊÔ£¬¿´¿´»á²»»á¶àһЩ rm(list = ls()) setwd(dir = "D:/R_project/UCR_project/") library(tidyverse) library(data.table) # ×¼±¸ÓÃÓÚVEP×¢Ê͵ÄvcfÎļþ UCRSNPPathogenic <- read.table(file = "02-analysis/06-UCR_Pathogenic_SNP/UCRSNPPathogenic.bed", ...
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options(stringsAsFactors = FALSE) library(ggplot2) library(reshape2) library(dplyr) library(stringr) library(lme4) library(lmerTest) library(RColorBrewer) library(ggpubr) library(parallel) library(MutationalPatterns) ref_genome="BSgenome.Hsapiens.UCSC.hg19" chr_orders=c(paste("chr",1:22,sep=""),"chrX","chrY","chrM") li...
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#!/usr/bin/env Rscript #### Fine cell type annotation of T cell subset for MBM_sc #### Author: Jana Biermann, PhD library(dplyr) library(Seurat) library(ggplot2) library(gplots) library(viridis) path.ct <- 'data/cell_type_DEG/MBM_sc/tcells/' filename <- 'MBM_sc_tcells' seu <- readRDS('data/cell_type_DEG/MBM_sc/main...
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output$genderBar <- renderEcharts4r({ if (!is.null(input$ageGenderOptionRegion) & !is.null(input$ageGenderOptionDateRange)) { dt <- GLOBAL_VALUE$signateDetail.ageGenderData updateDay <- max(dt$公表日, na.rm = T) dt[年代 == "", 年代 := "不明"] # TODO データ作成時処理すべき # 性別・年代マスター作成 genderAgeMaster <- data.table( ...
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tarbase.prepare <- function(TarBaseFile = NULL){ tarbase <- read.csv(Tar.dir, sep = "\t") mir.sets <- tarbase %>% filter(.,species == "Homo sapiens", positive_negative == "POSITIVE" ) mir.sets <- mir.sets %>% dplyr::select(.,mirna,geneId) %>% group_split(.,mirna...
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# Siwei 03 Jul 2023 # plot Xiaotong's data in dot plot format # init library(ggplot2) library(ggnewscale) library(scales) library(readxl) library(RColorBrewer) library(stringr) # plot the 3 cell types ##### Xiaotong_3_cell_types <- read_excel("plot_Xiaotong/Xiaotong_all_cell_types.xlsx") df_to_plot <- Xiaotong_3_...
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#' fastcluster #' #' Performs a fast two-step clustering: first clusters using k-means with a very #' large k, then uses louvain clustering of the k cluster averages and reports #' back the cluster labels. #' #' @param x An object of class SCE #' @param k The number of k-means clusters to use in the primary step (shoul...
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library(Rtsne) library(dbscan) library(ggplot2) library(DescTools) cleanMatrixForClusterW <- function(mtx, f_row = 0.5, f_col = 0.5) { cat(sprintf("Filter rows with >%1.2f missingness and columns with >%1.2f missingness.\n", f_row, f_col)) cat("Before: ", nrow(mtx), "rows and ", ncol(mtx),"columns.\n...
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## Functions to identify and plot DMRs from EWAS results ## # run.dmrff() - applies dmrff() to EWAS results # plotDMR() - calls miniman() to plot DMR results from run.dmrff() # plotDMR_wrap() - a wrapper for plotDMR() library(dmrff) library(data.table) source("DMR_miniman.r") # contains miniman function epicMan...