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3a68b82c19eab0a7fe9aa2dbcc470e42513ebb937b7cec1ba816d3bc4c402f52
R
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# try again and track time of model fitting in R instead of julia # use || to keep it reasonable library(dplyr) #library(lme4) library(tictoc) data <- tar_read(data_eegnet) %>% filter(experiment=="ERN") %>% select(-c("experiment")) tic() model <- lme4::lmer(accuracy ~ ( ref + hpf + lpf + emc + mac + base + det + ar)...
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R
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#' Count Cases or Blood Counts #' #' Function to count number of individual cases and blood counts. #' #' @param x `data.table`, in the format described in [`sbcdata`]. #' @return `integer`, number of cases or blood counts #' @author Sebastian Gibb <mail@@sebastiangibb.de> #' @rdname counts #' @export #' @examples #' c...
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R
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58
# sociodem EDA demo <- tar_read(demographics) model <- tar_read(eegnet_HLM) # TODO, also branches orig_data <- tar_read(data_eegnet) # from rfx_vis function data <- ranef(model)$subject %>% mutate(subject = rownames(.)) %>% mutate(intercept = `(Intercept)`) %>% select(c(intercept, subject)) rownames(data) <- ...
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R
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#' Label CBCs #' #' Run the labeling as done in the AMPEL project. #' #' @param x `data.table`, in the format described in [`sbcdata`] #' @param time `numeric(1)`, label entries with `"SecToIcu"` #' below this time and `Diagnosis == "Sepsis"` as "Sepsis" or as "Control" #' otherwise. #' @return `data.table`, same as `x...
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R
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#### load packages #### targetPackages <- c('tidyverse','biomaRt') newPackages <- targetPackages[!(targetPackages %in% installed.packages()[,"Package"])] if(length(newPackages)) install.packages(newPackages, repos = "http://cran.us.r-project.org") for(package in targetPackages) library(package, character.only = T) ###...
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R
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library(Seurat) library(tidyverse) library(stringr) setwd("~/cortex/fig4/") gene_list <- c( "PDZD2", "GNAL", "GRIA4", "CALB1", "DCC", "TRHDE", "GRIN3A") chipIDs <- cortexMeta %$% ChipID t2 <- list() bins <- list.files("../GEF/bins/", "200.qs", full.names = T) # bin200 seurat obejct mcla...
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R
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library(tidyverse) setwd("~/cortex/SnRNA/4_abaundance_differential/") library(data.table) library(magrittr) # subclass neuron test # cluster test wholeMeta <- read.csv("../3_mergingDatasets/SnRNA_Meta.csv") region <- c("ACC", "AG", "DLPFC", "FPPFC", "ITG", "M1", "PoCG", "S1", "S1E", "SMG", "SPL", "STG",...
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R
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#' Function to reverse the edge direction of a direct acyclic graph (DAG) #' #' \code{oDAGreverse} is supposed to reverse the edge direction of a direct acyclic graph (DAG; an ontology). The return graph remains all attributes associated on nodes and edges. #' #' @param g an object of class "igraph" or "graphNEL" #' @...
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R
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library(Seurat) library(parallel) library(stringr) library(tidyverse) library(magrittr) library(hdf5r) library(qs) setwd("~/cortex/STEREO/GEM") h5adFiles <- list.files("~/cortex/STEREO/GEM/bin200", "\\.h5ad", full.names = T) file = h5adFiles[[1]] mclapply(h5adFiles, mc.cores = 5, function (file) { print(file) dat...
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R
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#!/usr/bin/env Rscript library(tidyverse) library(ggplot2) library(Seurat) library(MAST) cell_type = commandArgs(trailingOnly=TRUE)[1] cleaned_cell_types <- c("L5/6_NP" = "L5/6 NP", "L5_ET" = "L5 ET", "L6_IT_Car3" = "L6 IT Car3", "L6_CT" = "L6 CT", "L6_IT" = "L6 IT", "L2/3_IT" = "L2/3 IT", "L4_IT" = "L4 IT", "L5_IT" ...
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R
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suppressPackageStartupMessages({ library(SC3) library(SingleCellExperiment) library(scater) library(aricode) }) ############################## Run sC3 ################################### run_SC3 <- function(sce){ if(ncol(sce) > 5000) { idx <- sample(1:ncol(sce), 5000) k <- sc3_estimate_k(sce[,id...
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R
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53
library(dplyr) library(data.table) library(optparse) # Load arguments ---------------------------------------------------------- # Environment: R 3.6.1 parse <- OptionParser() option_list <- list( make_option('--plink', type='character', help="plink-format files", action='store') ) args = commandArgs(t...
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R
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library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/gtex/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/gtex/output/" dds <- readRDS(paste(OUT_DIR,"dds.Rds",sep="")) vst.counts <- DESeq2::vst(dds, ...
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R
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library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/tcga/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/tcga/output/" dds <- readRDS(paste(OUT_DIR,"dds.Rds",sep="")) vst.counts <- DESeq2::vst(dds, ...
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R
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## ggpairs with adj correlation p values ## ## # Install and load the necessary packages library(GGally) library(dplyr) # Sample data data(mtcars) # Custom function to calculate correlations with adjusted p-values # Custom function to calculate correlations with adjusted p-values cor_with_p_adjust <- function(dat...
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R
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60
# TODO # other model checks (HLM) # posterior predictive check performance::check_model(hlmexp[[1]]) # model checks plot(model) # non-normality might / not must obscure statistical tests qqnorm(resid(model)) qqline(resid(model)) qqplot.data(resid(model)) # Extract random effects at level 1 random_effects <- ra...
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R
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require(plyr) require(dplyr) require(tidyverse) require(tidyr) require(reshape2) require(data.table) options(scipen = 999) #### ## EXPAND PEAKS AND WRITE AS BED FILE #### # Load grouped CREs cres = readRDS('OUT_DATA/Evo_groups/CREs_SignTested_ConsAdded.RDS') # Top N CREs n = 20000 type = 'TOP20K_E...
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R
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# READ DDS OBJECT FROM RDS FILE: ----------------------------------------------- dds <- readRDS( file = rds_deseq2_results ) # EXTRACT TRANSFORMED VALUES: -------------------------------------------------- vsd <- vst(dds, blind=FALSE) # CUSTOMIZE A PCA PLOT: -------------------------------------------...
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R
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setwd("~/cortex/STEREO/2_Deconvolution_and_QC/") chipList <- read.delim("~/DATA/data/STEREO/AnalysisPlot/cortex") %>% {setNames(nm = .$chip,.$region)} chipList2 <- read.delim("~/DATA/data/STEREO2/sampleMeta") %>% filter(cortex_category == "neocortex") %>% {setNames(nm = .$chipID,.$abbr)} chipList2[str_detect(chipList2...
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R
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context('Test Poisson regression model') set.seed(1994) test_that("Poisson regression works", { data(mtcars) bst <- xgb.train( data = xgb.DMatrix(as.matrix(mtcars[, -11]), label = mtcars[, 11], nthread = 1), nrounds = 10, verbose = 0, params = xgb.params(objective = 'count:poisson', nthread = 2) ) ...
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R
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#!/usr/bin/env Rscript ############################### #I/O ############################### library(tidyverse) library(ggplot2) library(Seurat) library(MAST) cell_type = commandArgs(trailingOnly=TRUE)[1] cleaned_cell_types <- c("L5/6_NP" = "L5/6 NP", "L5_ET" = "L5 ET", "L6_IT_Car3" = "L6 IT Car3", "L6_CT" = "L6 CT", "...
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R
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run.DESeq2 <- function (counts, target, varInt, batch = NULL, locfunc = "median", fitType = "parametric", pAdjustMethod = "BH", cooksCutoff = TRUE, independentFiltering = TRUE, alpha = 0.05, ...) { dds <- DESeqDataSetFromMatrix(countData = counts, colData = target, ...
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R
1,560
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args <- commandArgs(TRUE) if(length(args)!=3) { print("Usage: \'R --slave --args <InputData> <output> <CopyNumberEstimatFile> < refine.R\'") q() } infile = args[[1]]; outfile = args[[2]]; cnfile = args[[3]] x_in = read.table(infile,header=TRUE); x_in$ratio = x_in$obs/x_in$expected cn = ...
0de082060e9528b0cb39d64aee6424d52e84059aba92374379af9e850a11e6db
R
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#' @title mergeMatrix #' @description Merge multiple matrix files into one. At least two matrix files should be specified. #' @param x Matrix 1 or a list of Matrix. #' @param y Matrix 2. #' @param ... More matrix files. #' @export setGeneric( name = "mergeMatrix", def=function(x, y, ...) standardGeneric("mergeMatr...
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R
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39
library(Seurat) library(dplyr) library(ggplot2) ## CFSE Transcriptome: UMAP visulaization and cell state abundance ## ## load data and subset: load("Processed_Objects/Inhibitory_datasets.Rdata") Inhibitory_datasets <- SetIdent(Inhibitory_datasets, value = "Experiment" ) CFSE <- subset(Inhibitory_datasets, ident = c("...
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R
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48
library(Seurat) library(tidyverse) library(ranger) set("~/cortex/figS1-6/") seu <- readRDS('../SnRNA/SnRNA_seurat.RDS') seu <- seu[,seu$class == "excitatory"] train_data <- seu@reductions$pca@cell.embeddings %>% as.data.frame() train_data["label"] <- seu$region %>% factor train.forest <- ranger(label ~ ., ...
29216bfc147d8e64871b0226ccd6e83dcfa15b1e1627a24422ca301f18e466ee
R
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42
library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP061240/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP061240/output/" dds <- readRDS(paste(OUT_DIR,"dds.Rds",sep="")) vst.counts <- DESeq2::vst(dds, ...
4d3fcd7dfa16a2b45f77680403b135ecfea16325f0d5657a0f5da514791a340e
R
1,575
42
library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP050223/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP050223/output/" dds <- readRDS(paste(OUT_DIR,"dds.Rds",sep="")) vst.counts <- DESeq2::vst(dds, ...
56eae68d61f08d630fc449e58bed560d7df4ac20dd2fbf42ab01aa1ea094e6aa
R
1,575
42
library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP042228/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP042228/output/" dds <- readRDS(paste(OUT_DIR,"dds.Rds",sep="")) vst.counts <- DESeq2::vst(dds, ...
a324c35f53b6f8eadc2cc224b0e40e473bbe8ffd7fe014a5b583220a87304f69
R
1,575
42
library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP035988/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP035988/output/" dds <- readRDS(paste(OUT_DIR,"dds.Rds",sep="")) vst.counts <- DESeq2::vst(dds, ...
ab4df6c3dda6e2bb85f7a4f430beb1fe28787e7a2d3a878b0e825a52072a8dad
R
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42
library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP049593/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP049593/output/" dds <- readRDS(paste(OUT_DIR,"dds.Rds",sep="")) vst.counts <- DESeq2::vst(dds, ...
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R
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# ============================================================================== # run_all.R - MASTER SCRIPT: RUNS THE FULL ANALYSIS PIPELINE # ============================================================================== # # PURPOSE: # Convenience wrapper that runs all analysis scripts in the correct order, # wit...
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R
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args <- commandArgs(TRUE) if(length(args)!=3) { print("Usage: \'R --slave --args <InputData> <FragmentLength> <output> < normalize.R\'") q() } infile = args[[1]]; fragLen = as.numeric(args[[2]]); outfile = args[[3]]; load.success = library(mgcv,logical.return=TRUE) if(!load.success){ q(sav...
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R
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# extract average decoding accuracy after baseline in sliding window approaches # #data <- tar_read(data_sliding) %>% filter(experiment=="ERN") %>% filter(times <= -0.6) data <- tar_read(data_sliding) experiments <- c("ERN", "LRP", "MMN", "N170", "N2pc", "N400", "P3") baseline_end = c(-0.2, -0.4, 0., 0., 0., 0., ...
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R
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#' SNV data from individual A1 #' #' Exemplary SNV data from individual A1 of the study Körber et al., Detecting and quantifying clonal selection in somatic mosaicism. The dataset is a list object, containing variant information in vcf format. #' #' @format ## `snvs` #' A list containing a data frame with 447 rows and...
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R
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# similar RFX across experiments? model <- tar_read(eegnet_HLM_exp, branches=1)[[1]] # TODO: use pattern, and then combine the results orig_data <- tar_read(data_eegnet_exp, branches=1) %>% filter(tar_group==1) # to get the EXP information # start here data <- ranef(model)$subject %>% mutate(Subject = rownames(...
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library(tidyverse) library(qs) library(parallel) library(magrittr) library(RANN) library(ggridges) library(dendextend) library(ggpubr) devtools::load_all("~/spacexr-master/") setwd("~/cortex/fig1/") spatialCellMeta <- read_csv("../STEREO/spatialCellMeta.csv") subclass_color <-c(AST = "#665C47", ENDO = "#604B47", ET =...
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# load packages library(MEAanalysis) # data processing source("hippocampal_data_processing.R") #################################################################################################################### # Single electrode analysis - Hippocampal Neuronal Cultures # well A6: 1 mM KCl agonist challenge, 45k cel...
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library(viridis) library(pheatmap) library(RColorBrewer) combined.df <- readRDS("~/combined_df.Rds") tissue.vec <- readRDS("~/tissue_vec.Rds") datasource.vec <- readRDS("~/datasource_vec.Rds") colFun <- colorRampPalette(RColorBrewer::brewer.pal(10,"Paired")) annotation_col.df <- data.frame(Datasource = datasource.ve...
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suppressPackageStartupMessages({ library(cidr) library(aricode) library(SingleCellExperiment) }) ################################ Drop out #################################### #drop out function dropout_sampling <- function(sce, drop_rate = 0, seed){ #set seed set.seed(seed) #dropout sampling gene_size ...
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--- title: "plots" output: html_document date: "2024-05-13" --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` ## decodubg time series ```{r} library(ggplot2) library(dplyr) library(targets) # import N170 sliding data data <- tar_read(data_sliding) %>% filter(experiment == "N170") # generate a ...
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R
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#' Discretize a real valued distribution #' @description This function performs minimum description length (MDL)-optimal histogram density estimation #' as described in Kontkanen and Myllymäki (2007) and returns the cutpoints found to give the best model #' according to the MDL principle. #' #' @references #' \itemiz...
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# https://github.com/amarinderthind/RNA-seq-tutorial-for-gene-differential-expression-analysis # get the first agrument as the filename filename <- commandArgs(trailingOnly = TRUE)[1] #filename <- 'daf-16_SVIP_daf-16.salmon.deseq2_results_padj0.05.csv' print(filename) # get the second argument as the comparison compa...
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# modified 2021/02/02 by CT changing size of text inside cells pairwiseScatterPlots <- function (counts, group, outfile = TRUE) { ncol <- ncol(counts) if (ncol <= 30) { if (outfile) png(filename = "figures/pairwiseScatter.png", width = cairoSizeWrapper(700 * ...
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library(sybil) library(writexl) library(stringr) library(data.table) ### configure path2model = "../data/IMB015.RDS" path2output = "../result/metabolic_fluxes.xlsx" ### define functions getMetaboliteProduction <- function(mod) { require(sybil) require(data.table) # MTF sol.mtf <- optimizeProb(mod, algorith...
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suppressPackageStartupMessages({ library(SingleCellExperiment) library(SINCERA) library(aricode) }) memory.limit(1e+10) ################################ Drop out #################################### #drop out function dropout_sampling <- function(sce, drop_rate = 0, seed){ #set seed set.seed(seed) #dropo...
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MAPlot <- function (complete, alpha = 0.05, outfile = TRUE,fc.cutoff=1) { ncol <- ifelse(length(complete) <= 4, ceiling(sqrt(length(complete))), 3) nrow <- ceiling(length(complete)/ncol) if (outfile) png(filename = "figures/MAPlot.png", width = cairoSizeWrapper(1800 * ...
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library(tidyverse) library(readxl) library(ggpubr) setwd("~/Desktop/R_scripts/") file_path <- "all_performance_metrics.xlsx" data <- read_excel(file_path) data <- data %>% rename(Score = F1) data_f1 <- data %>% select(Method, Score, HistoneMark) %>% mutate(Metric = "F1") data_precision <- data %>% select(Method, Pre...
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R
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############################################### ############################################### ############################################### ############################################### ############################################### ############################################### ################################...
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R
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#' Function to calculate the area under the curve #' #' \code{oAUCurve} is supposed to calculate the area under the curve. #' #' @param x numeric vectors giving the x-coordinates #' @param y numeric vectors giving the y-coordinates #' @param method the medthod used to calculate the area under curve. It can be 'trapezoi...
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# DEFINE FILES AND PATHS: ------------------------------------------------------ # Path to the gene raw counts matrix input_cts <- "data/processed_data/rnaseq_e17/raw_counts/rnaseq_e17_raw_counts.tsv" # Path to the sample sheet file input_coldata <- "data/meta_data/rnaseq_e17/coldata.csv" # Path to output directory f...
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R
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library(viridis) library(pheatmap) library(RColorBrewer) combined.df <- readRDS("~/combined_df.Rds") tissue.vec <- readRDS("~/tissue_vec.Rds") datasource.vec <- readRDS("~/datasource_vec.Rds") colFun <- colorRampPalette(RColorBrewer::brewer.pal(10,"Paired")) annotation_col.df <- data.frame(Datasource = datasource.ve...
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R
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# DEFINE FILES AND PATHS: ------------------------------------------------------ # Path to the gene raw counts matrix input_cts <- "data/processed_data/rnaseq_e16/raw_counts/rnaseq_e16_raw_counts.tsv" # Path to the sample sheet file input_coldata <- "data/meta_data/rnaseq_e16/coldata.csv" # Path to output directory f...
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library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() TCGA_IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/tcga/input/" TCGA_OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/tcga/output/" GTEX_IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/gtex/input/" GTEX_OUT_DI...
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# test interactions #DEBUG data = tar_read(data_eegnet_exp, branches=1) %>% filter(experiment == "ERN") interactions = TRUE # INFO: Julia should start its own process by each function call, so the ENV variables set should be individual julia_library("Parsers, DataFrames, CSV, Plots, MixedModels, RData, CategoricalAr...
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library(readxl) library(dplyr) library(ggplot2) library(tidyr) library(ggstatsplot) library(patchwork) file_path <- "~/Downloads/final_peak_analysis_summary_all.csv" if (!file.exists(file_path)) { stop("File not found: ", file_path) } data <- read.csv(file_path) colnames(data) <- tolower(colnames(data)) data$me...
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### Analysis of genes or gene-sets of interest from Visium 10X datasets library(spacexr) library(Seurat) library(Matrix) library(doParallel) library(ggplot2) library(plyr) library(data.table) library(ggpubr) ############################################################################################ #### Inputs #####...
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## ----setup, include=FALSE----------------------------------------------------- knitr::opts_chunk$set(echo = TRUE) ## ----library, include=TRUE---------------------------------------------------- library(TockyPrep) library(TockyRandomForest) library(gridExtra) ## ----files, include=TRUE------------------------------...
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library(data.table) library(tidyr) library(ggplot2) library(ggrepel) #' Compare two different metrics files #' useful when tweaking pipeline or metrics new_metrics <- fread('data/metrics_batches_isolabel-fix.csv') # most recent metrics output old_metrics <- fread('data/metrics_batches_master.csv') # previous metric...
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# DEFINE FILES AND PATHS: ------------------------------------------------------ # Path to the gene raw counts matrix input_cts <- "data/processed_data/rnaseq_ctrl_e13/raw_counts/ctrl_e13_e17_counts.tsv" # Path to the sample sheet file input_coldata <- "data/meta_data/rnaseq_ctrl_e13-vs-e17/SampleSheet_e13_e17.csv" #...
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args <- commandArgs(TRUE) if(length(args)!=3 && length(args)!=4 ) { print("Usage: \'R --slave --args <InputData> <output> <fragmentLen> <CopyNumberEstimatFile> < refineGAM.R\'") q(save="no",status = 1) } infile = args[[1]]; outfile = args[[2]]; fragLen = as.numeric(args[[3]]); if(length(arg...
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# JULIA tests # https://hwborchers.github.io library(dplyr) library(JuliaCall) #options(JULIA_HOME = "~/Programs/julia-1.8.5/bin/") options(JULIA_HOME = "/Users/roman/.julia/juliaup/julia-1.10.2+0.aarch64.apple.darwin14/bin/") julia_setup() julia_call("sqrt", 2.0) julia_command("a = sqrt(2.0)") ## [1] 1.41421356237...
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#################################################################################################################### # R script to compare outputs between MEAanalysis results and Axis Navigator #################################################################################################################### #######...
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#' Function to find the root node of a direct acyclic graph (DAG) #' #' \code{oDAGroot} is supposed to find the root node of a direct acyclic graph (DAG; an ontology). It return the name (i.e Term ID) of the root node. #' #' @param g an object of class "igraph" or "graphNEL" #' @return #' \itemize{ #' \item{\code{ro...
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R
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# Load library if (!require("vioplot")) install.packages("vioplot") library(vioplot) # Trait columns trait_cols <- c( "gestation_length_d_x_brain_mass_g", "gestation_length_d_x_adult_mass_g", "litter_size_n_x_gestation_length_d" ) trait_labels <- c("GL X BrM", "GL X BM", "GL X LS") trait_colors <- c("#F9766E", "...
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library(CellChat) library(tidyverse) library(Seurat) library(qs) library(magrittr) options(stringsAsFactors = FALSE) setwd("~/DATA/BRAIN/STEREO/frequentGraph") geneIDtoSym <- read.csv("../../SnRNA/gene_kept.csv") %>% {setNames(.$gene_name,nm = .$gene_id)} scRNA <- qread("../../SnRNA/3_5_seu.rna.rmXY.qs") Idents(scR...
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R
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library(purrr) library(magrittr) library(tidyverse) library(Seurat) library(harmony) library(ape) library(uwot) library(ggtree) library(treeio) # library(future) setwd("~/cortex/SnRNA/3_mergingDatasets/") set.seed(123) qsFiles <- list.files(".","merge.qs",full.names = T) sampledCell <- function(x){ set.seed(1) x...
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# PREPARE DATA: ---------------------------------------------------------------- # Extract the numeric data for plotting starting at column 7 ctrl_data <- ctrl_matrix[, 7:ncol(ctrl_matrix), with = FALSE] nicd_data <- nicd_matrix[, 7:ncol(nicd_matrix), with = FALSE] # Calculate the mean across all regions for each posi...
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R
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library(tidyverse) setwd("~/cortex/SnRNA/3_mergingDatasets/") library(data.table) library(magrittr) resFiles <- list.files("./","merge.qs") x = resFiles[[1]] Meta <- parallel::mclapply(resFiles,mc.cores = length(resFiles),FUN = function(x){ seu <- qs::qread(x) seu@meta.data$sex[str_detect(seu@meta.data$donor,"^S[...
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R
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#' plot power curve #' #' @param resFile - the dataframe with proportions of probes with certain level of power output from running calcProps() where each column is a #' cell type and each row is a different condition, e.g. different sample size or mean difference #' @param calcType - character string of the type of p...
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library(Matrix) library(Seurat) library(stringr) library(magrittr) # library(SeuratDisk) library(tidyverse) library(Matrix) library(qs) library(parallel) devtools::load_all("~/spacexr-master/") setwd("~/cortex/STEREO/1_Deconvolution_and_QC/") qsFiles <- list.files("../cellbin/", ".qs$", full.names = T) snRNA = readR...
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library(dplyr) library(data.table) library(optparse) # Load arguments ---------------------------------------------------------- # Environment: R 3.6.1 parse <- OptionParser() option_list <- list( make_option('--bim', type='character', help="plink-format .bim file", action='store') ) args = commandArgs...
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suppressPackageStartupMessages({ library(SingleCellExperiment) library(SINCERA) library(aricode) }) memory.limit(1e+10) ################################ Drop out #################################### #drop out function dropout_sampling <- function(sce, drop_rate = 0, seed){ #set seed set.seed(seed) #dropo...
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library(magrittr) OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP035988/output/" labelled_edge_weights.df <- read.table(paste(OUT_DIR,"labelled_edge_weights.csv",sep=""), stringsAsFactors = FALSE,sep = ",",header = TRUE) rxn_ensembl_counts.nls <- readRDS(paste(OUT_DIR,"...
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# Run 'analysis/01_deseq2_e16.R' if you haven't already #source("analysis/01_deseq2_e16.R") # DEFINE FILES AND PATHS: ------------------------------------------------------ # input deseq2 results rds file rds_deseq2_results <- "data/processed_data/rnaseq_e17/r_objects/deseq2_dds_e17.rds" # output directory output_dir...
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R
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library(Seurat) library(tidyverse) library(stringr) setwd("~/cortex/fig2/") domainColor <- c( ARACHNOID = "#8a3b35", L1 = "#8ba28e", L2 = "#9ec87e", L3 = "#669c68", L4 = "#67b8bb", L5 = "#5687ac", L6 = "#5d5d8d", WM = "#b5b3bb" ) gene_l...
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# Run 'analysis/01_deseq2_e16.R' if you haven't already #source("analysis/01_deseq2_e16.R") # Run 'tables/scripts/tableS1.R' if you haven't already # source("tables/scripts/tableS1.R") # DEFINE FILES AND OUTPUT DIRS: ------------------------------------------------ # input rds file containing deseq2 results rds_deseq...
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library(magrittr) OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/gtex/output/" IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/gtex/input/" X <- readRDS(paste(OUT_DIR, "rxn_pca_nls.Rds", sep = "")) Y <- readRDS(paste(OUT_DIR,"gtex_tissue_detail_vec_train.Rds",sep="")) E <- read.table(paste(IN_DIR,"React...
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#code to generate Figure14 figure supplement 2 of the Platynereis 3d connectome paper #Gaspar Jekely Feb-Dec 2022 #load natverse and other packages, some custom natverse functions and catmaid connectivity info source("code/Natverse_functions_and_conn.R") imgTEM <- readPNG("pictures/Girdle_TEM_VNC_40um.png") panelTE...
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# Run 'analysis/04_deseq2_e13-vs-e17.R' if you haven't already #source("analysis/04_deseq2_e13-vs-e17.R") # DEFINE FILES AND PATHS: ------------------------------------------------------ # input deseq2 results rds file rds_deseq2_results <- "data/processed_data/rnaseq_ctrl_e13/r_objects/deseq2_dds_e13-vs-e17.rds" # o...
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#' Function to create a bipartitle graph #' #' \code{oBicreate} is supposed to create a bipartitle graph. #' #' @param data a data frame/matrix to create a bipartitle graph #' @param verbose logical to indicate whether the messages will be displayed in the screen. By default, it sets to true for display #' @return It r...
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############################################################################## # Figure description: Dotplot displaying combinatorial binding # Stage specific ATAC-seq peaks were used for performing tfcomb analysis # (https://tf-comb.readthedocs.io/en/latest/examples/TFBS_from_motifs.html) library(ggplot2) library(d...
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# code to generate the cell type connectivity matrix of the 3 day old Platynereis larva # Gaspar Jekely 2022-2023 source("code/libraries_functions_and_CATMAID_conn.R") # load the cell type graph generated in the Figure4 code syn_tb <- readRDS("source_data/Figure4_source_data1.rds") names <- syn_tb %>% activate(nod...
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library(qs) library(magrittr) library(MASS) library(tidyverse) library(sf) library(Seurat) library(patchwork) setwd("/home/luomeng/data/STEREO/AnalysisPlot//") seu <- qs::qread("merge_slides_seu.qs") # fig7a --------- SpatialDimPlot(seu,group.by = "domaine.fine") %>% ggsave("fig7a.pdf",height = 10,width = 20,limitsi...
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# ======================================================================= # FIG 4 - Sleepiness across Stages Plotting Script # ----------------------------------------------------------------------- # Variables: # time = 1:T # timepoints # sleep_onset = sf; # time index of sle...
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# setup_environment.R # Load renv for package management if (!requireNamespace("renv", quietly = TRUE)) { install.packages("renv") } library(renv) # Restore the environment from the renv.lock file if (file.exists("renv.lock")) { cat("\nRestoring environment from renv.lock...\n") renv::restore() } else { stop(...
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library(Seurat) library(data.table) library(ggplot2) library(plotly) library(ggforce) library(rtracklayer) library(ggtree) library(tidyverse) library(magrittr) library(patchwork) library(qs) library(parallel) library(ggtern) library(sf) setwd("~/data/STEREO/AnalysisPlot/") chipID = chipIDs[[1]] gtf <- import("../Homo...
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#' Merge lab entries #' #' Merge laboratory measurements. #' #' @param x `data.table`. #' @param f `factor`, splitting factor #' @param columns `character`, column names of laboratory measurements in `x`. #' @return `data.table` depending on `x` with reduced number of rows. #' @author Sebastian Gibb <mail@@sebastiangib...
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library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP035988/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP035988/output/" SRP035988_DATA_FIL <- "rse_gene.Rdata" ensembl2rxns.df <- read.table(paste(IN_DIR...
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#' @title Selection of ions with potential labeling signals #' @description Compare the average ion intensities of M2 and M3 isotopologue #' between labeled and unlabeled groups by ratio analysis. #' #' @param df The updated table generated after running Scanint. #' @param Intensity The minimum intensity ratio indi...
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R
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s <- data.table( Id = c(rep(1, 3), 2), Diagnosis = c(rep("Sepsis", 3), "SIRS"), Center = rep("G", 4), SecToIcu = c(1, -1, -1, NA), Sender = c("ED", "CIMC", "SICU", "MICU"), TargetIcu = c("CIMC", "CIMC", "SICU", "MICU"), CRP = c(NA, NA, 100, NA), HGB = seq(6, 8, length.out = 4) ) test_th...
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library(DESeq2) library(magrittr) library(SummarizedExperiment) start_time <- Sys.time() IN_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP061240/input/" OUT_DIR <- "/home/jgburk/PycharmProjects/reticula/data/SRP061240/output/" SRP061240_DATA_FIL <- "rse_gene.Rdata" ensembl2rxns.df <- read.table(paste(IN_DIR...
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#### load packages #### targetPackages <- c('tidyverse','arrow') newPackages <- targetPackages[!(targetPackages %in% installed.packages()[,"Package"])] if(length(newPackages)) install.packages(newPackages, repos = "http://cran.us.r-project.org") for(package in targetPackages) library(package, character.only = T) #### ...
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library(Seurat) library(parallel) library(stringr) library(tidyverse) library(magrittr) library(hdf5r) library(dplyr) library(rjson) library(Seurat) library(ggplot2) library(argparser) library(SeuratDisk) setwd("~/cortex/STEREO/1_cellbin/") h5adFiles <- list.files("./batch_gem/", ".h5ad$", full.names = T) mclapply(h...
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# Title : Install all R integration methods # Created by: mumichae # Created on: 6/4/21 suppressPackageStartupMessages({ library(optparse) library(data.table) }) optparse_list <- list( make_option( c("-d", "--dependencies"), type = "character", help = "Dependency TSV file with R packages & versi...
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#!/usr/bin/env Rscript ############################### #I/O ############################### library(tidyverse) library(ggplot2) library(Seurat) library(MAST) #add in info about tle status seurat_obj <- readRDS("/n/groups/walsh/indData/Maya/FCD_project/analysis/2_Analyze_Full_Object/Output/Seurat_Objects/8_processed_se...
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rm(list = ls(all.names = TRUE)) #will clear all objects includes hidden objects. gc() #free up memory and report the memory usage. library(tidyverse) library(tidygraph) library(stats) segmental_colors <- brewer.pal(6, 'Paired') #read graph conn.tb <- readRDS("supplements/connectome_graph_tibble.rds") conn.tb conn....
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library(tidyverse) setwd("~/cortex/fig4/") library(data.table) library(magrittr) library(Seurat) subclass_color <-c(AST = "#665C47", ENDO = "#604B47", ET = "#CEC823", CHANDELIER = "#E25691", `L2-L3 IT LINC00507` = "#07D8D8", `L3-L4 IT RORB` = "#09B2B2", `L4-L5 IT RORB` = "#69B19...
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#!/usr/bin/env Rscript ############################### #I/O ############################### library(tidyverse) library(ggplot2) library(Seurat) library(MAST) #add in info about tle status seurat_obj <- readRDS("/n/groups/walsh/indData/Maya/FCD_project/analysis/2_Analyze_Full_Object/Output/Seurat_Objects/8_processed_se...
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######read files suppressPackageStartupMessages({ library(SC3) library(SingleCellExperiment) library(scater) library(aricode) }) ################################ Drop out #################################### #drop out function dropout_sampling <- function(sce, drop_rate = 0, seed){ #set seed set.seed(seed...