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library(genio) library(data.table) library(magrittr) library(dplyr) library(devtools) library(R.utils) library(susieR) library(ggplot2) library(glue) ### Credit: Xiangyu Zhang ### args = commandArgs(trailingOnly=TRUE) gene <- args[1] gwas <- args[2] celltype <- args[3] weight <- as.numeric(args[4]) namelist <- rea...
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srun --nodes=1 --ntasks-per-node=1 --pty bash -i sif=/data/dcibioinformatics/sifs/dcibioinformaticsR-v2.1.sif wd=/data/dcibioinformatics/Anders/MelBrM_rerun singularity exec \ --containall \ --no-home \ --bind ${wd}:${wd}:rw \ ${sif} \ R # -----------------------------------------...
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# L2/3 IT neurons #################################################### # Cux2 Cre # Experiment ID: 294434161 # http://connectivity.brain-map.org/projection/experiment/294434161?imageId=156742363&initImage=TWO_PHOTON&x=19348&y=14762&z=4 # # # URL_projection_density = # "http://api.brain-map.org/grid_data/download...
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# UMAP plotting for samples, markers and clusters # adapted from SIMPLI plotting functions # Input data: # - clustered_cells.csv (SIMPLI output) ####### SETUP from SIMPLI ####### library(data.table) library(uwot) library(ggplot2) #install.packages("qs", type = "binary") library(qs) #source("scripts/Plot_Functions.R...
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#This is R code for a practice search in GEO #2026-06-22 #Megan Hagenauer ######################## if (!require(BiocManager, quietly = TRUE)) install.packages("BiocManager") BiocManager::install("GEOquery") library(GEOquery) #Come back to protocols.io and add quotes to "BiocManager" and "GEOquery" #protocols.io:...
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## Overlap between Tg2576, TauCortex, and human data across the main cell types ## rm(list = ls()) library(dplyr) # dplyr_1.1.4 library(ggplot2) # ggplot2_3.5.1 library(ggVennDiagram) # ggVennDiagram_1.5.2 options(stringsAsFactors = FALSE) options(width=120) set.seed(1) # Thy-Tau22 (17m) # load("./results/files/TauC...
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#!/usr/bin/env R # Author: Sean Maden # # Get RMSE calculations compatiable with test datasets. # # # labelsVectorFromFilter <- function(filterLabels, filterConditions, labels = c("sample.id", "condition.id", "replicate.id")){ # labelsVectorFromFilter # # Bind filter term labe...
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--- title: "Coeff vs DA corr" output: html_document editor_options: chunk_output_type: console --- # motus ```{r} library(ggplot2) library(dplyr) library(readxl) df.cross<-readRDS("/metaG//R_analyses/Taxonomy/RDS/df.DA.rds") df.pos.neg<-readRDS("/metaG//R_analyses/Taxonomy/RDS/Ridge_df.pos.neg.rds") # select only ...
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# R Corbett 2023 # # Generate alluvial plot for PBTA ancestry project # load libraries and set directories library(data.table) library(tidyverse) # library(ComplexHeatmap) # library(RColorBrewer) # library(circlize) library(colorblindr) library(ggpubr) library(ggalluvial) library(cowplot) root_dir <- rprojroot::find_...
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# creates final plots for the Simulations of the PRS GXE project # grab command line arguments args = commandArgs(trailingOnly = TRUE) print("Arguments received:") print(args) if(length(args) < 2) {stop("not enough Arguments received")} baseLoc = args[1] outputLoc= args[2] plotName= args[3] # Debug vars # baseLoc ...
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#Script to create Figure 2 library(Seurat) library(tidyverse) library(gdata) library(slingshot) library(RColorBrewer) library(sctransform) library(glmGamPoi) library(cowplot) set.seed(1000) #Huwe1 data ---- #Script to create Figure 1 - Huwe1 data #pseudotime construction using PCA for Huwe1 NSCs load(file = "1st_sub...
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# generate T4b gallery and save to pdf ---------------------------------------- # choose nb via nb_ind # align to meridian by mapping ref points to med via np library(natverse) library(tidyverse) source("eyemap_func.R") load('data/T4_gallery.RData') load('data/hexnb_ind_dist.RData') # in 'eyemap_lens_med_lop.R' nb...
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############################################## # Giotto Benchmarking - Kidney 10x Visium # Authors: Chandrima Modak, Mauminah Raina ############################################## #Load required packages library(Matrix) library(Seurat) library(Giotto) library(GiottoData) library(dplyr) library(readxl) libra...
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#!/usr/bin/env Rscript options(stringsAsFactors=F) ## load R libraries #library(SAIGE, lib.loc="../../install_dir/0.38") #library(SAIGE, lib.loc="../../install_dir/0.36.6") #library(SAIGE, lib.loc="/net/hunt/zhowei/project/imbalancedCaseCtrlMixedModel/Rpackage_SPAGMMAT/installSAIGEFolder/0.44.6_Neff") #library(SAIGE,...
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--- title: "Figure 5" author: "Sang LIU" date: "`r Sys.Date()`" output: html_document: code_download: TRUE theme: united --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE, warning=FALSE, message=FALSE) ``` ## Load packages ```{r} library(circlize) library(nat) library(ggpubr) library(reshape2...
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#!/usr/bin/env R # Author: Sean Maden # # Deconvolution plots script. Sourced by notebook for Ybulk experiment with 4 identifier conditions in the experiment. # deconvo_plot_statistics <- function(dfp1){ # postprocess dfp1 dfp1$s.fraction.neuron.glial <- dfp1$s.neuron/dfp1$s.glial dfp1$s.fraction.neuron.glial.d...
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--- title: "6. FAQ" output: rmarkdown::html_vignette: toc: true vignette: > %\VignetteIndexEntry{FAQ} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE, eval = FALSE) ``` # FAQ Below is an FAQ ## Hardware recommendations Be...
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--- title: "EDCS access and merging of TSV files" author: - Petra Hermankova^[Aarhus University, petra.hermankova@cas.au.dk, https://orcid.org/0000-0002-6349-0540] date: "`r format(Sys.time(), '%d %B, %Y')`" output: html_document: theme: united toc: yes toc_float: true number_sections: true toc_de...
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--- title: "Plate reader growth curves of GAT201 targets in RPMI" author: "Liz Hughes & Edward Wallace" date: "16/05/2023" output: html_document: toc: yes toc_depth: 2 --- ```{r call_shared_functions_formatting, child = "shared_functions_formatting.Rmd"} ``` # Summary Plate reader growth curves of GAT201 t...
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--- title: "Compare Coefficient of variation between individuals" author: "Joyce Hsiao" date: 2015-10-16 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") library(knitr) opts_chunk$set(message = ...
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rm(list=ls()) args <- commandArgs(TRUE) DATATAG <- args[1] # "Chinese_AgeT_Global" Indexflag <- args[2] # TotalGM ## COMMON LIBRARIES AND FUNCTIONS source("100.common-variables.r") source("101.common-functions.r") source("300.variables.r") source("301.functions.r") ## SCRIPT SPECIFIC LIBRARIES ## SCRIPT SPECIFIC F...
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library(dplyr) library(readxl) library(ggplot2) library(ggrepel) library(DOSE) library(enrichplot) library(clusterProfiler) library(org.Mm.eg.db) setwd("") # Figure_5b, bar plots of identified and quantified proteins, respectively { rm(list=ls()) Obj.list = readRDS(file = "ct_dataset_01.rds") temp.df = Obj.list...
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library(tidyr) library(dplyr) library(data.table) library(parallel) library(ComplexHeatmap) library(circlize) library(viridis) library(ggplot2) workingDir <- '../2024_02_29_multiVelo/' dataDir <- '../2024_02_29_multiVelo/data/' print("Reading in state files") stateFile_case <- fread(file.path(dataDir,'PFC_vs_VIS_Maca...
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#Example Code for Comparing Two Sets of Differential Expression Results #Megan Hagenauer #2025-06-11 ############### #Reading in the differential expression results for the two datasets: #Set your working directory to where your differential expression results are stored #This can be done using the GUI: "Session"->"...
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--- title: "RT-qPCR 2022-10-14 GAT201 complemented strains and targets" author: "Liz Hughes & Edward Wallace" date: "07/10/2022" output: html_document: toc: yes toc_depth: 2 --- ```{r call_shared_functions_formatting, child = "shared_functions_formatting.Rmd"} ``` # Experimental Design This experiment was ...
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#Determining which mouse genes align to which rat genes #Gene Ortholog Database: #Downloaded from Jackson Labs July 27, 2023 #https://www.informatics.jax.org/downloads/reports/HOM_AllOrganism.rpt #Another good source of ortholog information is HCOP: #https://www.genenames.org/tools/hcop/ #I should note: when deter...
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## Comparison with retro-seq data in vmPFC (Liqun LUO, Cell, 2020) ## 18 projection subtypes in PL/ILA/ORBm Label_Subtype_mPFC_PT = c(57, 60, 62, 64) Index_mPFC_PT = base::intersect(which(Label_Subtype_PFC %in% Label_Subtype_mPFC_PT == TRUE), which(PFC_projection$BrainRegion %in% c("PL...
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--- title: "Compare Coefficient of variation between individuals" author: "Joyce Hsiao" date: 2015-10-16 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") library(knitr) opts_chunk$set(message = ...
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--- title: "Explore distribituions" author: "Joyce Hsiao" date: 2016-02-01 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") library(knitr) opts_chunk$set(message = FALSE, warning = FALSE, eval...
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#Sample code for MetaData extraction for Gemma datasets, part2: Filtering #Megan Hagenauer, Jun 27 2024 ############ #This code has not been cleaned up, fully annotated, functionalized, and made easily generalizable ############### library(gemma.R) library(dplyr) ############### #After we've decided on our search...
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gen_histograms_spec_beh <- function(dir_in, hem, other_dat){ # generate histograms for key behavioral variables ids <- read.table(paste0(dir_in, '/', hem, '_final_ids.txt')) colnames(ids)[colnames(ids) == 'V1'] = 'id' other_dat <- inner_join(ids, other_dat, by = 'id', multiple = 'first') tps <- c('inf', 'tod', 'pre...
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# calculate time passed get_times <- function(data) { data_bl <- get_uniq_data(data) for (i in seq_len(nrow(data_bl))) { subj <- data_bl[i, "SubID"] bl_age <- data_bl[i, "chron_age"] is_subj <- (data$SubID == subj) data[is_subj, "time"] <- data[is_subj, "chron_age"] - bl_age } return(data) } #...
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Index_MOs_PT = base::intersect(which(PFC_projection$BrainRegion == "MOs"), Index_PT) NumNeuron_MOs_PT = length(Index_MOs_PT) ## main subtypes pie(table(Label_Subtype_PFC[Index_MOs_PT])) Index_MOs_PT_Main <- Index_MOs_PT[Label_Subtype_PFC[Index_MOs_PT] %in% c(56, 53, 58)] ## sear...
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#This is code that provides functions for aligning our differential expression results across datasets #Megan Hagenauer July 25 2024 #Goals: #Each dataset has differential expression results from a slightly different list of genes #Depending on the exact tissue dissected, the sensitivity of the transcriptional profili...
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#Fig 4b phee_count<-phee.analysis%>% dplyr::mutate(subject= factor(subject))%>% #dplyr::mutate(wks= factor(wks))%>% dplyr::mutate(timebin= factor(timebin, level = c("1min","2min", ...
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#' Proprocess a trial results tibble #' #' This function takes a trial results tibble as generated by `read_study_data` #' and adds a number of trial-level variables and summary statistics used for #' quality checks and analyses. Assumes that `threat_appear_time` has already #' been computed (as this can be study- and ...
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libv <- c("snow", "dplyr", "parallel", "doParallel", "lute", "dplyr") sapply(libv, library, character.only = T) #------ # setup #------ # helper functions # get series of s cell size factors (THIS SCRIPT, AND A FEW OTHERS) dfs.series <- function(s.glial.series = seq(1, 10, 0.2)){ s.neuron.series <- rev(s.glial.seri...
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--- title: Accessibility, Methylation, and 5hmC Boxplots --- ```{r warning=FALSE, message=FALSE} library(ggplot2) library(gghighlight) library(RColorBrewer) library(tidyverse) library(DESeq2) library(egg) peak_assignment<- read_tsv("/Users/lindsey/Desktop/Hodges_Lab/ATAC-me\ NPCdiff/NPCDiff_ATACme/New_timepoints/data...
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#Determining which mouse genes align to which rat genes #This code was updated April 25, 2024 #Gene Ortholog Database: #Downloaded from Jackson Labs April 25, 2024 #https://www.informatics.jax.org/downloads/reports/HOM_AllOrganism.rpt #Another good source of ortholog information is HCOP: #https://www.genenames.org/to...
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rm(list = ls()) library(plotly) library(tidyr) library(ggrepel) library(gtools) library(data.table) library(gplots) library(useful) library(locfit) library(Matrix) library(dplyr) library(ggplot2) library(Seurat) library(Rmagic) library(readr) library(phateR) library(viridis) #library(DoubletFinder) library(harmony) lib...
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--- title: "Effect of sequencing depth on UMI counts from Islam et al., 2014" date: 2015-09-24 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") opts_chunk$set(cache = FALSE) ``` Does the sequenc...
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library(shiny) library(shinydashboard) library(shinyalert) library(dplyr) library(purrr) library(ggplot2) library(echarts4r) library(MetBrewer) library(forcats) library(DT) library(zip) library(reticulate) library(Rcpp) library(reshape2) library(colormap) library(patchwork) library(qgraph) library(devt...
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# Created on Wed May 12 00:03:40 2021 # Name: synaptic_gradient_3d.R # Purpose: Colors neurons skeletons based on the synaptic gradient # Author: Andrea Vaccari (avaccari@middlebury.edu) # # Generates 5 plots: # - the first two plots are gradients for single posts based on the number of # synapses with pre. The c...
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--- title: DESeq2 for Differential Expression --- ```{r warning=FALSE, echo=FALSE, message=FALSE} library("tidyverse") library("DESeq2") library("ggplot2") library( "genefilter" ) library("pheatmap") library("cluster") library("factoextra") library("RColorBrewer") library(viridis) ``` ```{r} setwd("/Users/lindsey/Desk...
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# -*- coding: utf-8 -*- #Author: Xiaoyan Wang #Copyright (c) 2024 __CarlosLab@CCMU/CIBR__. All rights reserved. FB_colors <- c("Neuron" = "palevioletred2", "Astrocyte" = "olivedrab2", "Microglia" = "mediumpurple1", "Oligo" = "skyblue", "OPC" = "chocolate1") ### cell type markers for forebrain, interbrain ...
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--- title: "Cell type proportion summaries" author: "Sean Maden" date: "2023-09-21" output: pdf_document: default html_document: default --- Cell type proportions from snRNAseq and RNAscope. ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) libv <- c("dplyr", "ggplot2", "GGally", "ggcorrplot") sapply...
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## Test some edge cases not covered in the example files ## # Test for error when file contains no "START" messages test_that("test_err_on_no_data", { expect_error(read.asc(I("hello\nthere"))) }) # Fully test raw colname generating code test_that("test_raw_colnames", { # Test monocular first info <- lis...
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sif=/mnt/data1/public/singularity/dcibioinformaticsR-v2.1.sif wd=/mnt/data1/workspace/Anders/MelBrM rtmp=/mnt/data1/workspace/Methods/Containers/MissingPackages/v2.1:/rtmp singularity exec --containall --no-home --bind ${wd}:${wd}:rw --bind ${rtmp} ${sif} R library(tidyverse) library(openxlsx) library(DESeq2) library(...
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--- title: "Load Data" output: html_document: default pdf_document: default --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE,message = FALSE) ``` ## Load multiomics package ```{r load package} library(multiomics) ``` ## Load TMT Nothobranchius data Use the function `LoadMultiExperiment` to org...
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getwd() setwd(directory) library(DESeq2) # cannot load pheatmap although package installed apparently, load heatmap instead # read what the fehler says, execute it (delete some folder from documents bla bla) and try to install again, works! install.packages("pheatmap") library(pheatmap) install.packages("ggplot...
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#This code document includes the code for a function that is designed to run a basic meta-analysis of Log2FC and sampling variance values using our previously generated objects MetaAnalysis_FoldChanges & MetaAnalysis_SV #Megan Hagenauer #Original version: July 25 2024 #In response to reviewers' comments, this function ...
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#!/usr/bin/env Rscript #### Adjust BP, other parameters using childsds package #Setup library(childsds) #Define a function for printing the date, time and what we are doing: print_now <- function(statement){ #Function that takes a statement and prints the date and time print(paste(statement, Sys.time(), sep = ': ...
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--- title: "Windowing Raw Sample Data" author: "Austin Hurst" date: "`r Sys.Date()`" output: rmarkdown::html_vignette vignette: > %\VignetteIndexEntry{Windowing Raw Sample Data} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} --- ```{r results="hide", message=FALSE} # Import libraries required for t...
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--- title: "3. Scripting & notebooks" output: rmarkdown::html_vignette: toc: true vignette: > %\VignetteIndexEntry{Scripting and scaling up} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} --- ```{r, include = FALSE} options(width = 300, rmarkdown.html_vignette.check_title = FALSE) knitr::opt...
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--- title: "Summary visualisation of cingulotomy cell densities" output: html_document: df_print: paged --- ```{r setup, include=FALSE} library(tidyverse) library(here) library(forcats) library(ggdark) options(scipen=999) set_here(path='..') theme_set(dark_theme_bw(base_size = 14) + theme(legend....
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--- title: "Data transformation" date: 2015-09-30 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") opts_chunk$set(fig.width = 8, fig.height = 8) ``` ```{r packages, message=FALSE} library("ggplo...
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--- title: "Tracking down bug in reads to molecules conversion - Attempt 02" author: "John Blischak" date: 2015-02-19 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") opts_chunk$set(cache = TRUE)...
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--- title: "Quality control plots" author: "PoYuan Tung" date: 2015-10-21 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") opts_chunk$set(cache = FALSE) ``` ## Input ```{r packages, message=FAL...
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#code for sup figure 7 #import libraries library(ggplot2) library(REdaS) library(patchwork) library(nlme) #Panels B-D #load data nonz_data <- read.csv("C:/Users/mbasn/Dropbox/Manuscript-Basnak/Nat Neurosci Aug 2024 revision/zenodo/Ext_data_fig_7_analysis/all_movement_data_bar_trial_including_rest_10_sec...
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#' Create Excel header style. #' #' Create Excel header style. Depends on the \code{openxlsx} package. All arguments have default values and are optional to allow parameters to be modified. #' #' @param fill Header foreground fill. #' @param color Header font color. #' @param font Header font name. #' @param siz...
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#Dealing with a lack of Summarized Experiment object in Gemma #Megan Hagenauer, July 18, 2023 #Dataset: GSE85136 - for Christabel #This is an RNA-Seq dataset #It isn't playing well with the summarized experiment or expression set dataset objects rm(SummarizedExperiment) SummarizedExperiment<-gemma.R::get_dataset_obje...
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#Example pipeline for aligning our results across datasets: #Megan Hagenauer #July 25 2024 ############ #Goals: #Each dataset has differential expression results from a slightly different list of genes #Depending on the exact tissue dissected, the sensitivity of the transcriptional profiling platform, the representat...
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--- title: "Ordering effect of the capture sites, all genes CPM normalized" author: "Joyce Hsiao" date: 2015-10-02 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") ``` ## Goal We investigated p...
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--- title: "BASiCS - 40,000 iterations" date: 2015-08-10 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") ``` We analyzed our single cell data with [BASiCS][] developed by [Vallejos et al., 2015...
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--- title: "Extended Data Figure 4" author: "Sang LIU" date: "`r Sys.Date()`" output: html_document: code_download: TRUE theme: united --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE, warning=FALSE, message=FALSE) ``` ## Load packages ```{r} library(nat) library(reshape2) library(ggplot2) l...
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--- title: "KO DA" author: "SRomano" output: html_document: toc: yes editor_options: chunk_output_type: console --- # Load packages ```{r setup} list.of.packages <- c("tidyverse", "ggplot2", "RColorBrewer", "meta", "genodds") new.packages <- list.of.packages[!(list.of.packages %in% installed.packages()[,...
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--- title: "Pseudobulk k2 with and without cell size rescaling, cohort1" author: "Sean Maden" date: "2023-09-17" output: pdf_document: default html_document: default --- ```{r setup, include=FALSE} libv <- c("here", "nlme", "lute", "ggplot2", "gridExtra", "dplyr", "ggforce", "MultiAssayExperiment", "Sin...
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# MD subdivision library(stringi) library(matrixStats) PFC_projection = read.csv(file = "/home/admin/Documents/YanLab/Task_Local/PFC_single_neuron_projectome/data_preparation/PFC_neuron_projection_v3.csv", header = TRUE, sep = ",") Index_PT_CT = which(PFC_projection$Type %in% c("PT", "CT") ...
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##### imports ##### devtools::document() devtools::load_all() box::use( eplp = escottpricelabpipelines[ simulate_y, read_config, binary_from_liability, upsample_individuals_independently, simulate_e2_e4, downsample_individuals ], jntr = janitor[clean_names, row_to_names], dplyr = dplyr[filte...
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brain_region_ggseg <- function(meas, hem, dir_in, indx, fdr_cor){ # Plots FreeSurfer-labeled brain regions using the ggseg package # For questions: theodore_turesky@gse.harvard.edu if (hem == 'lh'){ hemi = 'left' } else { hemi = 'right' } # Specify color(s) to use labels_colors <- graph_labels_colors(meas)...
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################################################### ################################################### #### EMBL-EBI Dementia Course #### #### Biomarkers and PRS #### #### Emily Simmonds & Valentina Escott-Price #### #### UKDRI at Cardiff University #### #### 10th April 2024 #### ##...
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--- title: "BASiCS analysis of Mouse ESC dataset" date: 2015-09-29 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") opts_chunk$set(cache = FALSE) ``` The first half of the analysis is the data c...
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library(tidyr) library(dplyr) library(data.table) library(parallel) library(ComplexHeatmap) library(circlize) library(viridis) library(RColorBrewer) library(patchwork) library(ggplot2) workingDir <- '../2024_02_29_multiVelo/' dataDir <- '../2024_02_29_multiVelo/data/' print("Reading in state files") stateFile_case <-...
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# see Fig_4.R for ED Fig.7A, ED Fig.7B, ED Fig.7E, ED Fig.7F # ED Fig.7D, size, T4d on eye -------------------------------------------------- LL <- 4 # choose type # - eye com_xyz <- as.matrix(lens_type[[LL]][, c("comx","comy","comz")]) v0 <- as.matrix(lens_type[[LL]][, c("x0","y0","z0")]) v1 <- as.matrix(lens_type...
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--- title: "Filter genes and low-quality single cells" date: 2016-01-18 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") opts_chunk$set(fig.width = 8, fig.height = 8) ``` ```{r packages, message...
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--- bibliography: refs.bib --- ```{r chunk-options, include=FALSE} source("chunk-options-paper.R") ``` ## Supplementary Figures ![**Supplementary Figure `r fig_supp_qc`. Removal of low quality samples.** Violin plots of the total read-counts of ERCC spike-in controls in (A) and the total molecule-counts in (B) in si...
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--- title: "04_simulations" author: "Sean Maden" date: "2023-11-22" output: pdf_document: default html_document: default --- Results from pseudobulk simulations using Plasmablast and Non-plasmablast cell proportions from Monaco et al 2019. ```{r setup, include=FALSE} libv <- c("lute", "ggplot2", "gridExtra", "re...
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#!/usr/bin/env Rscript # Aaron M. Allen, 2019.06.06 library(Seurat) library(dplyr) library(future) plan("multiprocess", workers = 8) plan() NumberOfCCAs <- 45 nGeneLowCutOff <- 200 nGeneHighCutOff <- Inf nUMILowCutOff <- 1200 nUMIHighCutOff <- 10000 ClusterResolution <- 12 MitoLowCutOff <- -Inf...
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################################################################################ #################### EMA Microglia GSEA Bubbleplot ############################# ################################################################################ #March 12, 2024 # load libraries library('pheatmap') library(ggplot2) li...
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--- title: "Figure 6" author: "Sang LIU" date: "`r Sys.Date()`" output: html_document: code_download: TRUE theme: united --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE, warning=FALSE, message=FALSE) ``` ## Load packages ```{r} library(circlize) library(nat) library(ComplexHeatmap) library(...
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#' @title #' Training #' @description #' Tranining broad class classifier #' @param stTrain a dataframe that matches the samples with category #' @param expTrain the expression matrix #' @param dLevel the name of the column that contains categories #' @param colName_samp the name of the column that contains sample name...
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--- title: "Batch effects of conversion efficiency" date: 2015-10-23 author: Joyce Hsiao & PoYuan Tung --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") # opts_chunk$set(cache = TRUE) ``` ## Obje...
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outputDiagGRM = function( GRMdiagfile = "", GRMdiagSamplefile = "", plinkFile = "", phenoFile = "", phenoCol = "", traitType = "binary", invNormalize = FALSE, covarColList = NULL, qCovarCol = NULL, sam...
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--- title: "Revision PRM" output: html_document date: "2024-07-02" --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) dir <- "/wins/ori/Antonio/SpectroDive/PRM_Revision_Science_Paper_WholeBrain/SecondTry_Good" #Create all the colors col_code <- c("Transcriptome"="#377EB8", "Proteome"="#E4...
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# Spatial distirbution between clusters # input data: # - all_cells-clusters.csv (SIMPLI output) # - UMAPs.rds (generated in 2b. UMAPs of clusters) # - clusters_labels.csv (metadata) ##### 0. Load libraries ###### #install.packages("tidyverse") library(tidyverse) library(reshape) library(data.table) library(dplyr) l...
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--- title: "Setup for using ngsplot" date: 2015-03-07 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") opts_chunk$set(cache = FALSE) ``` [ngsplot][] calculates coverage of genomic regions. It pr...
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--- title: "metaG_Figs" author: "SRomano" output: html_document editor_options: chunk_output_type: console --- # load packages ```{r} library(ggplot2) library(dplyr) ``` # Load the data ```{r} # clr s5<-readRDS("/metaG/R_analyses/Taxonomy/RDS/Single_5per_log.rds") s10<-readRDS("/metaG/R_analyses/Taxonomy/RDS/Singl...
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#!/usr/bin/env R ### Author: Sean Maden ### ### Get diagram of deconvolution classes from lute. ### ### 1. A. deconvolutionParam ### 2. B. referencebasedParam ### 3. C. independentbulkParam ### 4. 1. nnlsParam ### 5. 2. musicParam ### 6. 3. epicParam ### 7. 4. deconrnaseqParam ### 8. 5. music2Param ### 9. 6. music2Par...
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#t-SNE plot(Fig.1D, Fig.S1C-D, Fig.2E) library(Seurat) library(monocle) library(ggplot2) DimPlot(sce_10X_integrated, reduction = "tsne.harmony",group.by = "harmony_clusters",pt.size = 0.2)+ scale_color_manual(values=palette(c("#98F7E4","#5F72E2","#985AB1", "#E6A0EA","#FDC471","...
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#### This code generates the middle section of figure 2 #panels A and B were generated separately in Matlab #panels H and I were generated separately in Python #load relevant packages library('broom') library('lme4') library('pracma') #panel C: HD encoding accuracy #load data offset_precision_data <- r...
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# sif=/mnt/data1/public/singularity/dcibioinformaticsR-v2.1.sif # wd=/mnt/data1/workspace/Anders/MelBrM # rtmp=/mnt/data1/workspace/Methods/Containers/MissingPackages/v2.1:/rtmp # singularity exec \ # --containall \ # --no-home \ # --bind ${wd}:${wd}:rw \ # --bind ${rtmp} \ # ${sif} \ # R set.s...
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--- title: "CDV LOSO" author: "SRomano" output: html_document editor_options: chunk_output_type: console --- # Load packages ```{r setup} library(phyloseq) library(dplyr) library(mlr3) library(SIAMCAT) require(mlr3extralearners) library(ggplot2) source("/Scripts/Cross_study_validation.r") source("/Scripts/Siamcat...
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#Script to create Figure 1 library(Seurat) library(tidyverse) library(gdata) library(slingshot) library(RColorBrewer) library(sctransform) library(glmGamPoi) library(cowplot) set.seed(1000) #Ascl1 data ---- #pseudotime construction using PCA for Ascl1 NSCs ---- load(file = "1st_submission/seurat_objects/Ascl1_qNSCs....
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--- title: "Normalize coefficients of variation" author: "Joyce Hsiao" date: 2015-10-15 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") library(knitr) opts_chunk$set(message = FALSE, warning = ...
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#Example pipeline for aligning our results across datasets: #Megan Hagenauer #July 27 2026 ############ #Goals: #Each dataset has differential expression results from a slightly different list of genes #Depending on the exact tissue dissected, the sensitivity of the transcriptional profiling platform, the representat...
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#'@export #'@title collapseMlist #'@description Takes in input a list of multiexperiment. In this case the complete #'mlist containing all the datasets, and a vector containing the names of the #'dataset you want to use, plus some other parameter coming from the shiny app #'control panel. It collapses the results in a...
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--- title: "Bortezomib Old" output: html_document date: "2024-06-25" --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` ## Load Settings ```{r} col_code_aging <- c(rev(RColorBrewer::brewer.pal(n = 4,"PiYG"))[c(3,1)],"#86b8e1","#e1868b", rev(RColorBrewer::brewer.pal(n = 4,"...
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--- title: "Quantify similarity between CVs" author: "Joyce Hsiao" date: 2015-10-31 --- **Last updated:** `r Sys.Date()` **Code version:** `r system("git log -1 --format='%H'", intern = TRUE)` ```{r chunk-options, include=FALSE} source("chunk-options.R") library(knitr) opts_chunk$set(message = FALSE, warning = FALS...
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--- title: "Shared functions and formatting" author: "Edward Wallace" date: '2023-05-16' output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE , warning=FALSE, message=FALSE) ``` ```{r load_packages, echo=FALSE} library(tidyverse) # data wrangling, ggplot, etc. library(reshape2) # ...
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#Code for generating simulations for Figure S1 of Warecki et al., 2024 ("Wolbachia infection reduces the abundance of the glutamate receptor mGluR and promotes female promiscuity and bacterial spread") #These simulations are based on experimental data generated in Figure 1 and provided in the file "grouped.mating.assay...