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9d512e7c2f03cebbc654c7f18fe26c9b7fc90713406a76e826f6b9952d36f0c9
R
530
12
options(timeout = max(300, getOption("timeout"))) if (("Rsubread" %in% rownames(installed.packages()) == FALSE) || (!require("Rsubread", quietly = TRUE))) { options(repos = c(CRAN="https://cloud.r-project.org/")) if (!require("BiocManager", quietly = TRUE)) { install.packages("BiocManager") } B...
e3bf57cb0cdc86cb8810dc2e537e98cc3810cff22121a84c2218b1273e06b01b
R
540
17
# file for storing variables filepath <- '' conditions <- c('AS', 'brain_tumours', 'celiac', 'CHD', 'colon_cancer', 'COPD', 'IBD', 'lung_cancer', 'lyme', 'MS', 'ovarian_cancer', 'pancreatic_cancer', 'parkinsons', 'PCOS', 'RA', 'SBE', 'schizophrenia', 'TB') results_filepath <- '' analysis_dt <- '2025-01-01' # sql...
2d1da40a5188a29bf9a39d08fa23f2d8c097fedf90d154b8d55d96b3b523578c
R
548
16
context("generate function") test_that('generate runs and returns expected output', { skip_on_cran() suppressWarnings(RNGversion(vstr = "3.5.0")) set.seed(42) vst_out <- vst(pbmc, return_cell_attr = TRUE) generated_data <- generate(vst_out) expect_equal(c(1, 0, 0, 4, 1), generated_data['ERP29', 1:5]) g...
669a63fe395151b966c147ddcf1175e71a16b536073bf19384c2a9da9879690a
R
580
13
# Migration to Python 3 The tool `2to3` was used as a first step. The code relied extensively on features deprecated in python3. * `zip()` now returns an iterable: in most of the code, this got replaced to `list(zip())`. * `gzip.open` objects (and use in contexts) handled strings in IO. In most places, the calls wer...
cb55e103c750eff14432705b41c26dcd0d690630580efb53284547c4e594245d
R
591
14
PanglaoDB <- read.csv("/Documents/R documents/SeuratExtend_databases/2020-3-27 PanglaoDB/PanglaoDB_markers_27_Mar_2020.tsv", sep = "\t") PanglaoDB_data <- list() Hs <- grepl("Hs",PanglaoDB$species) PanglaoDB_data[["marker_list_human"]] <- split(PanglaoDB$official.gene.symbol[Hs], PanglaoDB$cell.type[Hs])...
b6dae330a527d97922e1d6d4f9d32467fb9d84cee18a8c22bdef6085a956b65d
R
598
18
### pd = read.csv("he_SraRunTable.txt",as.is=TRUE) ## number of unique variables? uniqueIndex = which(sapply(pd, function(x) length(unique(x))) > 1) apply(pd[,uniqueIndex], 2, table) table(pd$Sample_Name, pd$tissue) fqPath = "/dcs04/lieber/lcolladotor/with10x_LIBD001/HumanPilot/Analysis/he_layers/FASTQ/" man = data...
ddd328e03bf40deef33df142e4c4cd757edc6f6ee3eda45d557f76a46eba5eba
R
616
21
#create separate databases for PTMSigDB based on the main 4 categories: PATH, PERT, KINASE, and DISEASE library(cmapR) library(glue) #database directory db.dir <- "../db/ptmsigdb/v2.0.0/all" #get all files gmt.files <- list.files(path=db.dir,pattern=".gmt") #for each file, split and save into correct directory sub.c...
ac7b95828d5648bee94e5f99ce6c9b4e7c745dc7f1e57bdc665f5cdc0042ac00
R
620
21
#!/usr/bin/env Rscript library(bambu) library(dplyr) library(rtracklayer) library(arrow) library(readr) args = commandArgs(trailingOnly=TRUE) input_rds <- args[1] bambu_result <- readRDS(input_rds) do.call(cbind, lapply(bambu_result, function(df) assays(df)$counts)) %>% as.matrix() %>% write.csv("bambu_expre...
3fef937f12d9d1e1708b5390e8a91ba27530a93f234e66f5416d82c65cb87ac8
R
626
18
library(IsoformSwitchAnalyzeR) aSwitchList <- readRDS("data/katherine/isoformswitch_inProg.rds") aSwitchList_part2 <- analyzeIUPred2A( switchAnalyzeRlist = aSwitchList, pathToIUPred2AresultFile = "export/iupred2a_processed_result.txt", showProgress = T) aSwitchList <- analyzeSignalP(aSwitchList, pathToSi...
c1b8dbb0f7adffea9eea8037a39f898fccee54d5f683e8d6b0a49d786a81ef3b
R
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28
#!/usr/bin/env Rscript library(bambu) library(rtracklayer) library(arrow) library(readr) args = commandArgs(trailingOnly=TRUE) annotation_gtf <- args[1] aligned_bam <- args[2] ref_genome_fasta <- args[3] ncore <- args[4] output_rds <- args[5] bambuAnnotations <- prepareAnnotations(annotation_gtf) bam_files <- Sys.glo...
ad2484fca4316cdc9c0ab1bf95d85337ddfed4bff5aaeeb35a297e44c370501c
R
655
18
library(GenomicRanges) library(GenomicFeatures) library(rtracklayer) library(dplyr) library(tidyr) gencode_exons <- makeTxDbFromGFF(paste0(Sys.getenv("GENOMIC_DATA_DIR"), "/GENCODE/gencode.v47.annotation.gtf")) %>% exonsBy(by = "tx", use.names = TRUE) %>% unlist() %>% unique() SFARI_exons <- rtracklaye...
4094f675cf13dff3a4b24f5aaca9592420c1ba52a4624c43a0a0028d5a970875
R
665
22
## f = list.files("Results", pattern = "gsa.out",full=TRUE) f = f[-5] # drop pgc3 names(f) = c("SZCD", "MDD", "ASD", "BPD") gsaList = lapply(f, read.table, header=TRUE, as.is=TRUE,row.names=1,comment="#") magmaTab = sapply(gsaList, "[[", "P") rownames(magmaTab) = rownames(gsaList[[1]]) magmaTab = magmaTab[!grepl("...
c20b83054ad452dd9c35ce450f1105fe6dc2f25bbcf11ce83045735cd46322ce
R
684
21
library(openxlsx) library(tidyr) library(dplyr) file <- "d:/Documents/R documents/SeuratExtend_databases/2021-5-21 preset colors/2021-8-12 preset colors I want hue.xlsx" col.space <- c("default","intense","pastel","all","all_hard") color_presets <- list() for (i in 1:5) { col_list <- read.xlsx(file, sheet = i, colNam...
0e24717f83dddca6a013820cb2bd51b03a839468a73369d88b1560c6f1eaf112
R
697
18
library(IsoformSwitchAnalyzeR) IsoseqsSwitchList <- readRDS("IsoseqsSwitchList.rds") IsoseqsSwitchList <- preFilter( switchAnalyzeRlist = IsoseqsSwitchList, geneExpressionCutoff = 1, # default isoformExpressionCutoff = 0, # default IFcutoff = 0.01, # default removeSingleIsoformGenes ...
d3eb5463f75ace2bf5ce19239f65131582c8c23336282bc20ddd0863e511c47b
R
715
22
library(IsoformSwitchAnalyzeR) library("BSgenome.Hsapiens.UCSC.hg38") bsg <- BSgenome.Hsapiens.UCSC.hg38 IsoseqsSwitchList <- readRDS("results/long_read/IsoseqsSwitchList.rds") IsoseqsSwitchList <- extractSequence( IsoseqsSwitchList, bsg, onlySwitchingGenes = FALSE, removeShortAAseq = FALSE, remov...
fb2ff187097f9f3a8bdf79c6fab3d632655decabd0dc1501c5d19d475791b732
R
736
38
--- title: "R Notebook" output: html_notebook --- ```{r} library(tidyverse) library(plotly) library(pROC) prl <- read.csv("socialBehaviorPrLdataPIindices.csv", stringsAsFactors = T) %>% mutate(ID = as.factor(ID), Savg = (S1 + S2)/2) ``` ```{r} # ROC analysis roc_obj <- roc(gt ~ Savg, da...
f02dadb65dbecbaea40a5621ddabac51918cfd2a303908596f9630142c497f9e
R
737
24
setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite/") # Import metadata ---- meta <- readRDS("Rdata/paper_metadata_v2.rds") # Sanity check overlap between sets (CV leakage) ---- meta[set %in% c("test", "validation", "training"), { # Import regions .c <- ....
b8ea9652641987e843a9fa40bf9c5ba515f96d0a948116da14049f0e39f17b8b
R
758
27
library(arrow) library(dplyr) library(ggplot2) library(ggpubr) peptide_mapping_for_plotting <- read_parquet("nextflow_results/V47/orfanage/peptide_mapping_for_plotting.parquet") peptide_mapping_for_plotting %>% ggplot( aes(x=GENCODE, y=mean_expression) ) + geom_boxplot() + theme_minimal() my...
10514a15cce0d8136640df1802e0b68f5debab37b8ee437f4aef0b84260041bc
R
764
32
# Function to compute AUC ---- vl_ROC_AUC <- function(label, predicted, plot.line= F, col= "red", ...) { if(!is.logical(label)) label <- as.logical(label) # Make data table ---- dat <- data.table(label= label, predicted= predicted) # Order ---- setorderv(dat, "predicted", -1) ...
a25cb6939f8f4d61c6179b44633b9fd03d49ccf874c43c5edcd7b03d52afa05c
R
775
30
context("lots-of-points") test_that("geom_text_repel works with 10,000 points and 32 labels", { cars <- mtcars cars$car <- rownames(mtcars) set.seed(42) dat3 <- rbind( data.frame( wt = rnorm(n = 10000, mean = 3), mpg = rnorm(n = 10000, mean = 19), car = "" ), cars[,c("wt", "mpg...
877cd9b88ab3aefc63610ece007c21c37bc0962724a6f66359e6583bdf0e2389
R
779
17
context("correcting") test_that('correcting runs and returns expected output', { skip_on_cran() suppressWarnings(RNGversion(vstr = "3.5.0")) set.seed(42) vst_out <- vst(pbmc, return_cell_attr = TRUE, res_clip_range = c(-Inf, Inf)) y_smooth <- smooth_via_pca(vst_out$y, do_plot = FALSE) expect_equal(c(910, 2...
dbcc3dcb19d91fc7dc1232b847b4ba7a49d7f16c7da7cad0b2761dbb3bac713f
R
801
33
##### Create read file ##### require(GenomicSEM) library(devtools) args = commandArgs(trailingOnly=TRUE) tissue <- args[1] #Tissue setwd("./12_TWAS/5_all_combined/4_im") # List files files <- list(paste0("AD_", tissue, "_ALL.dat"), paste0("ASTH_", tissue, "_ALL.dat"), paste0("IBD_", tissue, "_ALL.dat"), paste0(...
121f173ecb968ddeea417a87d6b86106b93e751756d02b06b963fdd1397504cc
R
802
25
library(tidyverse) #Set correct working directory setwd("D:/RW-PCB 11 Astrocyte Imaging/Files for Cropping and Analysis/Analysis Files/Somatosensory/GFAP_Mask_Measurements") files <- list.files(pattern = "\\.csv$", full.names = TRUE) files <- files[file.info(files)$size > 0] summary_dataset <- map_dfr( fi...
627553cb044229d210e986533e31145f82456ec533d9785eff2887a068bf6906
R
805
18
setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite-dev/") # List observed files ---- dat <- data.table(TL= paste0("TL", c(12, 9, 13))) dat <- dat[, .(obs.file= list.files(paste0("db/observed/bulkATAC/", TL, "/"), recursive = T, full.names = T)), TL] dat[, tiss...
ecf5ddd8f9605947fe0f1babd67cc57b8a699ddcb7143193568ce8d42dc128d1
R
807
21
setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite/") # Import data ---- dat <- readRDS("db/folds/bulkATAC_folds.rds") dat <- dat[group=="vista"] # Add missing labels ---- add <- readxl::read_xlsx("/groups/stark/shenzhi.chen/db/VISTA_enhancer_dataset/VISTA202...
857b33d6835f4e9dcfed3072aca41604ec819d3d2798a344cc757a82c1258cbc
R
815
33
##### Create read file ##### require(GenomicSEM) library(devtools) args = commandArgs(trailingOnly=TRUE) tissue <- args[1] #Tissue setwd("./12_TWAS/5_all_combined/1_cvd") # List files files <- list(paste0("AF_", tissue, "_ALL.dat"), paste0("CAD_", tissue, "_ALL.dat"), paste0("HF_", tissue, "_ALL.dat"), paste0("...
9b47c45ed45e79c91bec179399168f3a99622940bf0a370f1fa117f9b4ff4077
R
818
26
library(ggplot2) library(readr) library(dplyr) library(tidyr) library(RColorBrewer) my_theme <- theme_classic() + theme( axis.title.x = element_text(size = 13), axis.title.y = element_text(size = 13), axis.text.x = element_text(size = 12), axis.text.y = element_text(size = 12) )...
bf9e09da3854e5f6fb2583e7662a6de2a98a833368dbeee7592c6dbad98c9f79
R
820
27
library(readr) library(dplyr) library(ggplot2) my_theme <- theme_classic() + theme( axis.title.x = element_text(size = 13), axis.title.y = element_text(size = 13), axis.text.x = element_text(size = 12), axis.text.y = element_text(size = 12), legend.position = "none" ) t...
5d143ffe91c581761af6155d0ac36bb7e2fe748ad5b4b129d4c7a714a8381f3b
R
821
18
# Open a connection to a log file logfile <- file("*PLACEHOLDERPATH*/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("DESeq2") count_data <- read.table("tests/test_files/big_counted.csv", header=TRUE, sep= ",", row.names = 1) des...
1037402bbd6a5314bb58903785b3e1de27a6d938392eab460b3f580652ea5ead
R
824
36
### library(jaffelab) library(Biostrings) x = read.csv("../10X/151675/tissue_positions_list.txt", as.is=TRUE, header=FALSE) x$barcode = ss(x$V1, "-") bcs = DNAStringSet(x$barcode) ## hamming dd_hamming = stringDist(bcs, method = "hamming") dd_mat_hamming = as.matrix(dd_hamming) dd_mat_hamming[upper.tri(dd_mat_ham...
db0c0f30520bc99d5b0d19e7a3023ae425dc2c7d362edf722850d7427b5138f3
R
827
23
# Open a connection to a log file logfile <- file("tests/test_files/featurecounts_tests/outdir/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("Rsubread") fc <- featureCounts(arg = TRUE, arg2 = NULL, arg3 = "string", arg4 = c("st...
2ec63138d5b20eb9481c1a07fc3551686d693cccf71d40967961f40ac4c61200
R
830
22
setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite/") # Import metadata ---- meta <- readRDS("Rdata/paper_metadata_v3.rds") meta <- meta[ID=="model1_bulkATAC_tsx3Aug_2xBal_noW"] # Select VISTA tiles and only the center ATAC-Seq bins meta <- meta[(dataset=="acc...
d0302874177c08668016effe265b99ad39e20aa9e49cf18c95f6a417bb1b55f6
R
835
34
##### Create read file ##### require(GenomicSEM) library(devtools) args = commandArgs(trailingOnly=TRUE) tissue <- args[1] #Tissue setwd("./12_TWAS/5_all_combined/3_gastro") # List files files <- list(paste0("GORD_", tissue, "_ALL.dat"), paste0("GSD_", tissue, "_ALL.dat"), paste0("IBS_", tissue, "_ALL.dat"), pa...
e255b120bc02e43d5ae3297165634f5fcb4cef2a3c14374ccf1441f4faf6d32a
R
841
25
options(timeout = max(300, getOption("timeout"))) pgks <- list("statmod") for (pkg in pgks) { tryCatch( tryCatch( {install.packages(pkg) require(pkg)}, warning = function(e) { install.packages(pkg, type = "binary")}, error = function(e) { install.packages(pkg...
4c96fd0c66819e348040f05137b842595f166e25c33da6c4d1d952ba789b019b
R
842
22
localSRMD <- function(unconstrained, constrained, lhsvar, rhsvar){ print(paste0("calculating localSRMD across h = ",length(unconstrained)/length(lhsvar[[1]])," parameters in g = ",length(lhsvar[[1]])," groups (",length(unconstrained)," parameters total)")) #convert lists to data frames lhsframe <- t(data.fr...
7201b1634205b70413b9cbdb8bc5200973d126ed2b8c76191332de9a3f572d9e
R
842
26
library(readr) library(dplyr) library(ggplot2) my_theme <- theme_classic() + theme( axis.title.x = element_text(size = 13), axis.title.y = element_text(size = 13), axis.text.x = element_text(size = 12), axis.text.y = element_text(size = 12), legend.position = "none" ) t...
a34d9a1df922e2f3b60a99568d0d2a7f0a1f18e57b516249d3c41eb00e4579c5
R
861
25
library(tidyverse) #Set correct working directory setwd("D:/RW-PCB 11 Astrocyte Imaging/Files for Cropping and Analysis/Analysis Files/Somatosensory/GFAP Morphology") files <- list.files(pattern = "\\.csv$", full.names = TRUE) files <- files[file.info(files)$size > 0] summary_dataset <- map_dfr( files, ...
7bf36f4b1faff3118515c90769127ca06ca30203765a3f6b6437136198a97c2d
R
876
24
setwd("~/Documents/SeuratExtend-database/2024-4-5 color pro from i want hue") files <- list.files(pattern = ".csv") files <- files[!grepl("_original",files)] theme <- c("default","light","red","yellow","green","blue","purple","bright") lines <- readLines(files[1]) color_palettes <- strsplit(lines, ",") color_palettes...
71ae1b04bb02901d3058860f528526c4a13d205c4f89995742c695a0ad6946a8
R
877
20
#' Peripheral Blood Mononuclear Cells (PBMCs) #' #' UMI counts for a subset of cells freely available from 10X Genomics #' #' @format A sparse matrix (dgCMatrix, see Matrix package) of molecule counts. #' There are 914 rows (genes) and 283 columns (cells). This is a downsampled #' version of a 3K PBMC dataset available...
f35069831e14870553545bdb5ed8f5decf13f655c56a78d2cf4e241b5a5c2c02
R
877
33
##### Create read file ##### require(GenomicSEM) library(devtools) args = commandArgs(trailingOnly=TRUE) tissue <- args[1] #Tissue setwd(".12_TWAS/5_all_combined/2_metab/") # List files files <- list(paste0("GSD_", tissue, "_ALL.dat"), paste0("HDL_", tissue, "_ALL.dat"), paste0("MDD_", tissue, "_ALL.dat"), past...
313d2f57bcefec58dd97a335e04dddd53a0e6430b1d52012ba6829c95cb8c7d0
R
889
21
library(ppcor) args <- commandArgs(trailingOnly = T) inFile <- args[1] outFile <- args[2] # input expression data inputExpr <- read.table(inFile, sep=",", header = 1, row.names = 1) geneNames <- rownames(inputExpr) rownames(inputExpr) <- c(geneNames) # Run pcor using spearman's correlation as mentioned in the PNI pa...
d04a54ffc8e8f70c36ee757be3ea75f01d7401406fea11097b690ed520ffc4c0
R
891
36
--- title: "R Notebook" output: html_notebook --- ```{r} library(openxlsx) library(tidyverse) # Read in the winsorized data with the additional variables. dat <- read.csv("winsorizedOutliersRemovedAdditionalVars.csv") # Read in social behavior data. wb <- loadWorkbook(file = "PrL_behavior_ML_2025.xlsx") s1 <- read....
fc97b138b989b5e1860a25e98d019d66687e43fe47bd3e71fee1b287ce4d3769
R
900
16
context("differential expression") # test_that('compare expression runs and returns expected output', { # skip_on_cran() # options(mc.cores = 2) # set.seed(42) # vst_out <- vst(pbmc, return_cell_attr = TRUE) # # create fake clusters # clustering <- 1:ncol(pbmc) %/% 100 # res <- compare_expression(x = vst...
08b1b9a7d21d853cc63b5c46305152cc425dc4ce33f8de92c67721326715b7ad
R
902
29
library(tidyverse) library(IsoformSwitchAnalyzeR) Isoseq_Expression <- read.csv("results/long_read/Isoseq_Expression.csv") sampleID <- colnames(Isoseq_Expression)[c(-1)] time_point <- str_split(sampleID, "_", 2) %>% map_chr(~ .x[1]) time_point <- factor(time_point, levels = c("iPSC", "NPC", "CN")) myDesign <- data.fr...
e81fda9f5082216ea3c2ff7bccfb059f88d2fcbc9b98c3fe0ece0420753b6134
R
903
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setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite/") cmd <- vlite::cmd_mergeBigwig( bw = c( "/groups/stark/shenzhi.chen/projects/transferLearningMammalianEnhancerDesign202408/clean_version/db/bw/forebrain_merged.CPM.bigwig", "/groups/stark/shenzhi.c...
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R
964
51
setwd("/Users/zhangyuan/Google Drive/2023_math_reading_neurotransmitter/GitHub") rm(list = ls()) # ------------------------------------------------------------ # Get R package versions for reporting summary # ------------------------------------------------------------ packages <- c( "CCA", "CCP", "ppcor", "B...
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R
979
21
# Open a connection to a log file logfile <- file("*PLACEHOLDERPATH*/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("DESeq2") count_data <- read.table("tests/test_files/big_counted.csv", header=TRUE, sep= ",", row.names = 1) des...
c60727366aa7c60b722bec74d093160de5bcec53a3ce3b8c6b48a749e5d2247c
R
980
36
#!/usr/bin/env Rscript library(Gviz) library(rtracklayer) library(GenomicFeatures) library(dplyr) options(ucscChromosomeNames=FALSE) args <- commandArgs(trailingOnly=TRUE) bam_file <- args[1] gtf_file <- args[2] gene_name <- args[3] gencode_gtf <- args[4] out_pdf <- args[5] gencode <- import(gencode_gtf)...
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R
982
38
##### T_SEM Script ###### require(GenomicSEM) library(devtools) args = commandArgs(trailingOnly=TRUE) tissue <- args[1] setwd("./12_TWAS/T_SEM") load("LDSCoutput_CVD.RData") genes <- readRDS(paste0("./6_read_fusion/1_cvd/CVD_", tissue, ".rds")) model1 <- " F_CVD =~ HF + CAD + STK + AF F_CVD_MDD =~ a*MDD + a*F...
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R
990
31
setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite/") # Import data ---- dat <- readRDS("db/folds/bulkATAC_folds.rds") dat <- dat[group=="vista"] # Add missing labels ---- add <- readxl::read_xlsx("/groups/stark/shenzhi.chen/db/VISTA_enhancer_dataset/VISTA202...
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R
997
32
setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite-dev/") # List peak files (reproducible from rep1 and rep2, see paper) ---- meta <- data.table( file= list.files( path = "/groups/stark/shenzhi.chen/projects/accessibility_model_enhancer_design_17112025/d...
cb4dd3cf4b102345bcb35eacc23a8109ec60c8f69f12431dac4619ae1d81e012
R
997
25
library(LEAP) args <- commandArgs(trailingOnly = T) inFile <- args[1] maxLag <- as.numeric(args[2]) outFile <- args[3] # input expression data inputExpr <- read.table(inFile, sep=",", header = 1, row.names = 1) geneNames <- rownames(inputExpr) rownames(inputExpr) <- c() # Run LEAP's compute Max. Absolute Correlation...
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##### T_SEM Script ###### require(GenomicSEM) library(devtools) args = commandArgs(trailingOnly=TRUE) tissue <- args[1] setwd("./12_TWAS/T_SEM") load("LDSCoutput_gastro.RData") genes <- readRDS(paste0("./6_read_fusion/3_gastro/Gastro_", tissue, ".rds")) model1 <- " F_gastro =~ GORD + GSD + IBS + PUD F_gastro_...
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R
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#!/usr/bin/env Rscript library(readxl) library(dplyr) library(rtracklayer) library(GenomicRanges) library(stringr) library(readr) args <- commandArgs(trailingOnly=TRUE) riboseq_file <- args[1] riboseq <- read_excel(riboseq_file, sheet = 2) %>% dplyr::rename( seqname = chrom, start = codon5, ...
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R
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# Open a connection to a log file logfile <- file("*PLACEHOLDERPATH*/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("DESeq2") count_data <- read.table("tests/test_files/big_counted.csv", header=TRUE, sep= ",", row.names = 1) des...
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R
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##### T_SEM Script ###### require(GenomicSEM) library(devtools) args = commandArgs(trailingOnly=TRUE) tissue <- args[1] setwd("./12_TWAS/T-SEM") load("LDSCoutput_IM.RData") genes <- readRDS(paste0("./6_read_fusion/4_im/IM_", tissue, ".rds")) model1 <- " F_IM =~ ASTH + AD + IBD + MS F_IM_MDD =~ a*F_IM + a*MDD...
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R
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########################################################## #This R script is to analysis the motif enrichment and cluster ########################################################## Args <- commandArgs() in_file = Args[6] out_file = Args[7] human_motif<-read.table(file=in_file, header = F, sep = "\t") head(human_mot...
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R
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library(dplyr) library(purrr) library(ggplot2) source("src/utils.R") novel_splice_site_ClinVar <- read_vcf("export/variant/novel_splice_site_ClinVar.vcf", info=c("CLNVC", "CLNSIG", "MC", "GENEINFO")) novel_splice_site_ClinVar %>% mutate(MC = str_remove(MC, ",.*$")) %>% mutate(MC = str_extract(MC, "(?<=\\|)[...
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R
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##### T_SEM Script ###### require(GenomicSEM) library(devtools) args = commandArgs(trailingOnly=TRUE) tissue <- args[1] setwd("./12_TWAS/T_SEM") load("LDSCoutput_metab.RData") genes <- readRDS(paste0("./6_read_fusion/2_metab/Metab_", tissue, ".rds")) model1 <- " F_metab =~ MSYN + T2D + TG + HDL + GSD F_metab...
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R
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options(timeout = max(300, getOption("timeout"))) if (("DESeq2" %in% rownames(installed.packages()) == FALSE) || (!require("DESeq2", quietly = TRUE))) { options(repos = c(CRAN="https://cloud.r-project.org/")) install.packages("png") pgks <- list("XML", "vctrs", "RCurl") for (pkg in pgks) { tryC...
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R
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options(width=100) library(scater) library(SingleCellExperiment) ## load annotated sce objects load("SCE_singlet-spots_MNT.rda") load("SCE_neuropil-spots_MNT.rda") dim(sce.singlet) dim(sce.neuropil) table(sce.singlet$subject_position, sce.singlet$prelimCluster_MNT) table(sce.neuropil$subject_position, sc...
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R
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# ============================================================================= # run_all.R - master driver. Runs the full pipeline in order. # Each script can also be run independently (later scripts read the .rds # intermediates written by earlier ones into data/processed/). # Rscript run_all.R # ==================...
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R
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library(ggplot2) library(dplyr) my_theme <- theme_classic() + theme( axis.title.x = element_text(size = 12), axis.title.y = element_text(size = 12), axis.text.x = element_text(size = 10), axis.text.y = element_text(size = 10), legend.position = "none" ) theme_set(my_the...
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R
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suppressPackageStartupMessages({library(data.table);library(IlluminaHumanMethylation450kanno.ilmn12.hg19)}) b <- fread("__MS_GEO_ROOT__/Methylation_Data/New_Datasets/GSE88824_beta_raw.csv") p <- b[[1]]; B <- as.matrix(b[,-1]); rownames(B) <- p ann <- getAnnotation(IlluminaHumanMethylation450kanno.ilmn12.hg19) xp <- row...
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R
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library(dplyr) library(rtracklayer) library(GenomicFeatures) library(GenomicAlignments) annotation_gtf <- paste0(Sys.getenv("GENOMIC_DATA_DIR"), "GENCODE/gencode.v47.annotation.gtf") SFARI_exon <- makeTxDbFromGFF(predicted_cds_gtf) %>% cdsBy(by = "tx", use.names=TRUE) # Read in the final_transcript GTF file gtf...
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R
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#!/usr/bin/env Rscript args = commandArgs(trailingOnly=TRUE) #' This functions takes sythetic data to test DSTG's performance #' @return This function returns files saved in folders "Datadir" & "Infor_Data" #' @export: all files are saved in current path #' @examples: load data from folder "syntheic_data" source('R_...
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R
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suppressPackageStartupMessages(library(BTR)) suppressPackageStartupMessages(library(doParallel)) num_cores = 32 #specify the number of cores to be used. doParallel::registerDoParallel(cores=num_cores) args <- commandArgs(trailingOnly = T) inFile <- args[1] inMdl <- args[2] maxGenesPerRule <- as.numeric(args[3]) allo...
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R
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#' Run association test uisng longutudinal data #' #' @param pheno : data frame of the phenotype #' @param outcome : as.numeric , outcome to test #' @param covars_prs : covariates to adjust #' @param random_effect : e.g Subject ID or FamilyID #' #' @return association test results in data frame #' @export #' #' @exa...
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R
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# Open a connection to a log file logfile <- file("tests/test_files/featurecounts_tests/outdir/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("Rsubread") fc <- featureCounts(files = c("tests/test_files/featurecounts_tests/single...
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R
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packages <- c( "Cairo", "CalNetExploreR", "ComplexHeatmap", "ComplexUpset", "NatParksPalettes", "RColorBrewer", "UpSetR", "bigstatsr", "broom", "circlize", "cowplot", "data.table", "devtools", "dplyr", "factoextra", "forcats", "furrr", "ggVennDiagram", "ggbiplot", "ggdendro", "...
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R
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#' Title : Genearte AUC #' #' @param pheno : data frame of the phenotype #' @param outcome : as.numeric , outcome to test #' @param covars_prs : covariates to adjust #' @return auc #' @export #' #' @examples #' generate_auc<-function(pheno,outcome, covars_prs){ pheno_df<-pheno[,c(outcome,covars_prs )] ...
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R
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library(GenomicRanges) library(GenomicFeatures) library(rtracklayer) library(dplyr) library(VariantAnnotation) library(tidyr) library(parallel) source("src/utils.R") novel_exonic_regions <- readRDS("export/variant/novel_exonic_regions.rds") gencode_exons <- makeTxDbFromGFF(paste0(Sys.getenv("GENOMIC_DATA_DIR"), "/GE...
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R
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library(arrow) library(stringr) library(DESeq2) library(dplyr) my_theme <- theme_classic() + theme( axis.title.x = element_text(size = 13), axis.title.y = element_text(size = 13), axis.text.x = element_text(size = 12), axis.text.y = element_text(size = 12), ) theme_set(my_theme...
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R
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setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite/") # Import validated synthtetic enhancer sequences ---- heart <- readRDS("Rdata/final_designed_enhancer_sequences_heart.rds") heart <- heart[id %in% c(311, 726, 834, 890, 845) & label=="ledidi_12_14"] limb <-...
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R
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PEER_plotModel <- function(model){ par(mfrow=c(2,1)) bounds = PEER_getBounds(model) vars = PEER_getResidualVars(model) par(mar=c(5,4,4,5)+.1) plot(bounds, type="l", col="red", lwd=2, xlab="Iterations", ylab="Lower bound") par(new=TRUE) plot(vars,,type="l",col="blue",xaxt="n",yaxt="n",xlab="...
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R
1,227
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library(ggplot2) library(scales) colorVector <- c( "FSM" = "#009E73", "ISM" = "#0072B2", "NIC" = "#D55E00", "NNC" = "#E69F00", "Other" = "#000000" ) structural_category_labels <- c( "full-splice_match" = "FSM", "incomplete-splice_match" = "ISM", "novel_in_catalog" = "NI...
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R
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# Open a connection to a log file logfile <- file("*PLACEHOLDERPATH*/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("DESeq2") count_data <- read.table("counted.csv", header=TRUE, sep= ",", row.names = 1) design_matrix <- read.ta...
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R
1,247
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## module load conda_R/3.6.x ## ----Libraries ------------------ library(parallel) library(SummarizedExperiment) library(Matrix) library(RColorBrewer) library(jaffelab) library(edgeR) ## load rse list load("Human_DLPFC_Visium_processedData_rseList.rda") ## filter to expressed genes, lets be liberal exprsMat = sappl...
4697a2fc024b702fbdc500afe4e83c57ea9e1eac4b400945cbbeea63b8f549a3
R
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#' ggrepel #' #' This package contains extra geoms for \pkg{ggplot2}. #' #' Please see the help pages listed below: #' #' \itemize{ #' \item \code{\link{geom_text_repel}} #' \item \code{\link{geom_label_repel}} #' } #' #' Also see the vignette for more usage examples: #' #' \code{browseVignettes("ggrepel")} #' #' P...
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R
1,258
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setwd("/groups/stark/vloubiere/projects/DeepATAC_shenzhi/") devtools::load_all("/groups/stark/vloubiere/vlite/") # Import data ---- dat <- readRDS("db/folds/bulkATAC_folds.rds") dat <- dat[group=="vista"] # Overlap ---- cols <- c("heart", "limb", "forebrain", "midbrain", "hindbrain", "neuralTube") # Upset plot pdf("...
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R
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# Open a connection to a log file logfile <- file("*PLACEHOLDERPATH*/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("DESeq2") count_data <- read.table("tests/test_files/big_counted.csv", header=TRUE, sep= ",", row.names = 1) des...
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R
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# Creates 10x1001bp tiles per region (5 + strand, 5 - strand) augTile <- function(bed, width= 1001, shifts= c(-400, -200, 0, 200, 400)) { # Hard copy ---- bed <- data.table::copy(bed) # Augment regions using tiling ---- aug <- lapply(shifts, function(x) { .c <- data.table::copy(bed) .c[, start:= (sta...
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--- title: "Fig 4m - Arp3 KO line profile, NMIIb-focus variant" output: html_notebook params: genotype: "Arp3KO" final_plot_channel: "Ch2" pdf_name: "Actin_profile.pdf" write_csvs: FALSE --- <!-- ================================================================================ Wrapper for Fig 4m, Arp3 KO geno...
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--- title: "Fig 4o - brightfield neurite-xcorr (Arp3 KO genotype)" output: html_notebook params: genotype: "KO" pixel: 0.1081075 freq: 0.8 cell_range_start: 10 cell_range_end: 18 cell_prefix: "Cell" --- <!-- ================================================================================ WRAPPER for Fig ...
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# Open a connection to a log file logfile <- file("tests/test_files/featurecounts_tests/outdir/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("Rsubread") fc <- featureCounts(files = c("tests/test_files/featurecounts_tests/paired...
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R
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library(ggplot2) #synthetic <- read.csv("~/R/data/DDLS/adni_synthetic_bl_2430.csv", header = TRUE, stringsAsFactors = TRUE) synthetic <- read.csv("~/Python/WASP-DDLS/DS-synthetic-data/degree3_root_AGE/bn_eps_100.csv", header = TRUE, stringsAsFactors = TRUE) synthetic$DX[which(synthetic$DX == "")] <- NA real <- read.cs...
4c438b9096fed1e54574f9c844cd261767fc824ee9fbf09b494e26d426f72d21
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1,309
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PEER_plotModel <- function(model){ par(mfrow=c(2,1)) #bounds = PEER_getBounds(model) bounds = (1:10) + rnorm(10) #vars = PEER_getResidualVars(model) vars = -(1:10)/10. + rnorm(10,0,0.1) par(mar=c(5,4,4,5)+.1) plot(bounds, type="l", col="red", lwd=2, xlab="Iterations", ylab="Lower bound") ...
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--- title: "Fig 4m - WT line profile, actin-focus variant" output: html_notebook params: genotype: "WT" final_plot_channel: "Ch3" pdf_name: "Act_profile.pdf" write_csvs: TRUE --- <!-- ================================================================================ Wrapper for Fig 4m, WT genotype, "actin focu...
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--- title: "ED Fig 11d-f - EB3 length under CK-666 (DIV-3, deconvolved images)" author: "Lin et al., Nature 2026 (Bradke lab, DZNE)" output: html_notebook params: div: 3 image_pipeline: "decon" summary_variable: "growth_speed_median" pdf_name: "EB3_length.eps" d_drive_source: "D:\\DVElite\\CK666_LA_EB3\\24022...
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--- title: "ED Fig 11d-f - EB3 length under CK-666 (DIV-4, raw images)" author: "Lin et al., Nature 2026 (Bradke lab, DZNE)" output: html_notebook params: div: 4 image_pipeline: "raw" summary_variable: "growth_speed_median" pdf_name: "EB3_length.eps" d_drive_source: "D:\\DVElite\\CK666_LA_EB3\\240226_DIV4_EB3...
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R
1,332
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--- title: "Fig 4m - WT line profile, NMIIb-focus variant" output: html_notebook params: genotype: "WT" final_plot_channel: "Ch2" pdf_name: "Act_profile.pdf" write_csvs: FALSE --- <!-- ================================================================================ Wrapper for Fig 4m, WT genotype, "NMIIb foc...
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R
1,334
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data <- read.csv("temp.csv", header =FALSE) D<-data[2] #head(D) block_length<- ceiling(nrow(D)^(1/3)) blocks <- D[1:block_length, 1] for (i in 2:(length(D[, 1]) - (block_length-1))) { blocks <- rbind(blocks, D[i:(i + (block_length-1)), 1]) } mbb_size<-floor(nrow(D)/block_length) # MOVING BLOCK BOOTSTRAP xbar <- NULL fo...
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R
1,341
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library(GenomicRanges) library(dplyr) library(readr) library(rtracklayer) library(arrow) detected_peptides <- import("nextflow_results/V47/orfanage/UCSC_tracks/detected_peptides.gtf") tx_classification <- read_parquet("nextflow_results/V47/final_classification.parquet") protein_classification <- read_tsv("nextflow_res...
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R
1,342
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--- title: "Fig 4m - Arp3 KO line profile, actin-focus variant" output: html_notebook params: genotype: "Arp3KO" final_plot_channel: "Ch3" pdf_name: "Actin_profile.pdf" write_csvs: TRUE --- <!-- ================================================================================ Wrapper for Fig 4m, Arp3 KO genot...
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1,356
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--- title: "Fig 4o - brightfield neurite-xcorr (WT genotype)" output: html_notebook params: genotype: "WT" pixel: 0.3243227 freq: 0.2 cell_range_start: 11 cell_range_end: 13 cell_prefix: "Cell_" --- <!-- ================================================================================ WRAPPER for Fig 4o, ...
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1,357
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--- title: "ED Fig 1b - 2D-culture pooled-replicate neurite-xcorr" output: html_notebook --- <!-- ================================================================================ WRAPPER for ED Fig 1b (2D culture, pooled-replicate CCF). ================================================================================...
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R
1,362
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### library(jaffelab) dir.create("S3") ## mv loupe files path = "/dcs04/lieber/lcolladotor/with10x_LIBD001/HumanPilot/10X/" ## copy barcodes etc h5 = list.files(path, pattern = "feature_bc_matrix.h5", recur=TRUE,full=TRUE) file.copy(h5, "S3/") hi = list.files(path, pattern = "image.png", recur=TRUE,full=TRUE) newhi ...
8d3a1a84b640ee4c04a383abe71ee68d8c17abe468a03b6532c9dbdadc6ede45
R
1,364
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# Open a connection to a log file logfile <- file("*PLACEHOLDERPATH*/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("DESeq2") count_data <- read.table("tests/test_files/big_counted.csv", header=TRUE, sep= ",", row.names = 1) des...
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R
1,375
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# Open a connection to a log file logfile <- file("*PLACEHOLDERPATH*/logfile.log", open = "a") # Redirect both output and messages to the file and console sink(logfile, append = TRUE, split = TRUE) require("limma") design_matrix <- read.table("tests/test_files/test_design_matrix.csv", header=TRUE, sep= ",") design_ma...
871c84bae46569c1b60b27d0406e9e4f17d1c6c8ab23651c341bf8b98306a3c1
R
1,380
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### library(jaffelab) library(filesstrings) ## unpack system("tar xvf Lieber_Visium_Transfer_1.tar") system("tar xvf Lieber_Visium_Transfer_2.tar") ## move to common folder system("mv Lieber_Visium_Transfer_1/ 10X") system("mv Lieber_Visium_Transfer_2/ 10X") system("mv Lieber_Visium_Transfer_2/Lieber/ Analysis/") ##...